Rroxscaffold_3G00220830

Endoplasmic reticulum-Golgi intermediate compartment protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011866
Physical Location & Seq
Reverse (-)
3336717 .. 3342541
5825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00220830.1

Sequence Viewer

Length: 1161 bp
ATGGAGAATATGATGAGCCGCCTCCGCAATTTGGACGCGTACCCCAAAATCAACGAAGATTTCTACAGCCGTACCCTCTCCGGCGGCGTCATCACCCTCGTCTCCTCCATCGTTATGCTCCTGCTCTTCCTCTCCGAGCTCCAGTTATATCTACATGCAGTGACGGAAACGAAGCTTGTGGTAGATACTTCGAGAGGAGAAACCCTGCGTATCAATTTCGATGTAACTTTTGCTGCGCTTCCTTGTTCTATACTCAGTCTTGATGCCATGGACATTAGTGGAGAGCAGCACCTTGATGTAAAACATGACATTATCAAAAAAAGAATAGACTCCAATGGAAATGTTATAGAATCAAGGCCGGATGGAATTGGTGCCCCCAAGATTGACAAGCCTTTACAGAGACATGGTGGCAGACTTGAGCACAATGAGACATACTGTGGTTCTTGTTACGGTGCTGAAGCGAATGATGAGGATTGTTGTAATAACTGTGAAGAGGTCCGTGAAGCTTACCGAAAGAAAGGTTGGGCCATTTCAAATCCAGATTTGATTGACCAGTGCAAAAGAGAAGGGTTTTTGCAAAAGATTAAAGATGAAGAAGGTGAAGGATGCAATATATATGGGTTCTTGGAAGTCAATAAGGTGGCCGGGAACTTTCATTTTGCACCTGGGAAAAGCTTTCAACAATCAAATGTTCATGTACATGATTTGCTGGCATTTCAAAAGGATAGTTTTAATATAAGTCACACAATCAACAGATTGTCTTTTGGAGACTATTTCCCTGGTGTTGTCAATCCTCTTGATGGGGTGCACTGGACACAGGACAACCCGAGTGGGATGTATCAATATTTTATCAAGGTTGTTCCTACTGTGTACACAGATGTCAGGGGGCACACTATCCAGTCAAATCAGTTCTCTGTCACTGAACATTATAGGGGAACAGAAATAGGCAACCTTCAGTTTCTTCCTGGAGTGTTTTTCTTCTATGACCTTTCCCCGATAAAGGTAACTTTCACGGAGGAGCATATTTCTTTCTTACACTTCCTGACAAATGTGTGTGCCATTGTTGGAGGCGTTTTTACCGTGTCTGGAATATTGGATTCGTTTATATACCATGGTCAGAAGGCAATTAAAAAGAAGATGGAAATTGGCAAATTCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N

Protein Analysis

386

Amino Acids

43.52

Weight (kDa)

5.75

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 8 - 97 9.1e-32 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
COPIIcoated_ERV PF07970 149 - 366 1.3e-88 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011866)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G36050 AT1G36050
fragaria_vesca FvH4_5g37810 FvH4_5g37810 FvH4_5g37810
malus_domestica MD08G1180300.v1.1 MD15G1365600.v1.1
prunus_persica Prupe.1G513700_v2.0.a1
pyrus_communis pycom08g15430 pycom15g32800
rosa_chinensis RchiOBHm_Chr7g0239691
rosa_laevigata RLG00000000741
rosa_multiflora Rmu_sc0000063.1_g000019
rosa_roxburghii Rroxscaffold_3G00220830
rosa_samantha Rh7AG482900 Rh7BG455600 Rh7CG500300 Rh7DG468500
rosa_wichuraiana Rw7G040420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 371
AccII CGCG 1 cut(s) 38
AciI CCGC 3 cut(s) 19, 25, 84
AcoI YGGCCR 1 cut(s) 642
AcsI RAATTY 1 cut(s) 1151
AcuI CTGAAG 2 cut(s) 477, 938
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 4 cut(s) 41, 73, 699, 872
AfiI CCNNNNNNNGG 3 cut(s) 31, 800, 1000
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 3 cut(s) 534, 680, 719
AjnI CCWGG 3 cut(s) 664, 778, 964
AjuI GAANNNNNNNTTGG 2 cut(s) 505, 537
AluBI AGCT 4 cut(s) 139, 175, 506, 675
AluI AGCT 4 cut(s) 139, 175, 506, 675
Alw21I GWGCWC 3 cut(s) 141, 423, 810
Alw26I GTCTC 4 cut(s) 106, 394, 422, 762
Alw44I GTGCAC 1 cut(s) 806
Ama87I CYCGRG 1 cut(s) 826
AoxI GGCC 3 cut(s) 356, 525, 642
ApaLI GTGCAC 1 cut(s) 806
ApeKI GCWGC 2 cut(s) 233, 286
ApoI RAATTY 1 cut(s) 1151
AspLEI GCGC 1 cut(s) 238
AspS9I GGNCC 2 cut(s) 496, 525
AsuC2I CCSGG 1 cut(s) 646
AsuHPI GGTGA 2 cut(s) 85, 611
AvaI CYCGRG 1 cut(s) 826
AvaII GGWCC 1 cut(s) 496
BaeGI GKGCMC 3 cut(s) 376, 810, 891
BaeI ACNNNNGTAYC 2 cut(s) 55, 88
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 141
Bbv12I GWGCWC 3 cut(s) 141, 423, 810
BbvI GCAGC 2 cut(s) 220, 298
BccI CCATC 4 cut(s) 116, 356, 794, 1132
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 3 cut(s) 666, 780, 966
BcnI CCSGG 1 cut(s) 646
BcoDI GTCTC 4 cut(s) 106, 394, 422, 762
BfmI CTRYAG 1 cut(s) 64
BisI GCNGC 4 cut(s) 19, 85, 234, 287
BlsI GCNGC 4 cut(s) 20, 86, 235, 288
Bme1390I CCNGG 4 cut(s) 646, 666, 780, 966
Bme18I GGWCC 1 cut(s) 496
BmeT110I CYCGRG 1 cut(s) 826
BmgT120I GGNCC 2 cut(s) 496, 525
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 4 cut(s) 646, 666, 780, 966
BmsI GCATC 2 cut(s) 253, 596
BpmI CTGGAG 2 cut(s) 125, 987
BpuEI CTTGAG 1 cut(s) 437
BpuMI CCSGG 1 cut(s) 646
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 4 cut(s) 267, 665, 778, 1111
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 800, 1000
Bse1I ACTGG 4 cut(s) 142, 553, 815, 898
BseBI CCWGG 3 cut(s) 666, 780, 966
BseDI CCNNGG 4 cut(s) 267, 665, 778, 1111
BseGI GGATG 3 cut(s) 367, 611, 840
BseLI CCNNNNNNNGG 3 cut(s) 31, 800, 1000
BseMII CTCAG 1 cut(s) 268
BseNI ACTGG 4 cut(s) 142, 553, 815, 898
BseRI GAGGAG 3 cut(s) 94, 210, 1031
BseSI GKGCMC 3 cut(s) 376, 810, 891
BseXI GCAGC 2 cut(s) 220, 298
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 3 cut(s) 358, 527, 644
BshNI GGYRCC 1 cut(s) 371
BsiHKAI GWGCWC 3 cut(s) 141, 423, 810
BsiHKCI CYCGRG 1 cut(s) 826
BsiSI CCGG 3 cut(s) 81, 359, 645
BslI CCNNNNNNNGG 3 cut(s) 31, 800, 1000
BsmAI GTCTC 4 cut(s) 106, 394, 422, 762
BsmBI CGTCTC 1 cut(s) 106
BsnI GGCC 3 cut(s) 358, 527, 644
BsoBI CYCGRG 1 cut(s) 826
Bsp1286I GDGCHC 5 cut(s) 141, 376, 423, 810, 891
Bsp1407I TGTACA 2 cut(s) 697, 870
Bsp19I CCATGG 2 cut(s) 267, 1111
BspACI CCGC 3 cut(s) 19, 25, 84
BspANI GGCC 3 cut(s) 358, 527, 644
BspCNI CTCAG 1 cut(s) 267
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 1 cut(s) 373
BspQI GCTCTTC 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 371
BsrGI TGTACA 2 cut(s) 697, 870
BsrI ACTGG 4 cut(s) 142, 553, 815, 898
BssECI CCNNGG 4 cut(s) 267, 665, 778, 1111
BssNI GRCGYC 1 cut(s) 87
BssT1I CCWWGG 2 cut(s) 267, 1111
Bst2UI CCWGG 3 cut(s) 666, 780, 966
Bst4CI ACNGT 5 cut(s) 437, 452, 488, 868, 1081
Bst6I CTCTTC 2 cut(s) 131, 486
BstACI GRCGYC 1 cut(s) 87
BstAUI TGTACA 2 cut(s) 697, 870
BstC8I GCNNGC 1 cut(s) 711
BstDEI CTNAG 1 cut(s) 254
BstDSI CCRYGG 2 cut(s) 267, 1111
BstF5I GGATG 3 cut(s) 367, 611, 840
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 1 cut(s) 238
BstMAI GTCTC 4 cut(s) 106, 394, 422, 762
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstNI CCWGG 3 cut(s) 666, 780, 966
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 4 cut(s) 644, 664, 778, 964
BstSFI CTRYAG 1 cut(s) 64
BstSLI GKGCMC 3 cut(s) 376, 810, 891
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 2 cut(s) 220, 298
BsuRI GGCC 3 cut(s) 358, 527, 644
BtgI CCRYGG 2 cut(s) 267, 1111
BtsCI GGATG 3 cut(s) 367, 611, 840
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 4 cut(s) 165, 560, 808, 918
Cac8I GCNNGC 1 cut(s) 711
CfoI GCGC 1 cut(s) 238
Cfr13I GGNCC 2 cut(s) 496, 525
CseI GACGC 2 cut(s) 44, 76
Csp6I GTAC 4 cut(s) 40, 72, 698, 871
CspCI CAANNNNNGTGG 2 cut(s) 811, 846
CviAII CATG 7 cut(s) 155, 268, 305, 404, 695, 701, 1112
CviQI GTAC 4 cut(s) 40, 72, 698, 871
DdeI CTNAG 1 cut(s) 254
EaeI YGGCCR 1 cut(s) 642
Eam1104I CTCTTC 2 cut(s) 131, 486
EarI CTCTTC 2 cut(s) 131, 486
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 2 cut(s) 267, 1111
Eco24I GRGCYC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 496
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 2 cut(s) 477, 938
Eco88I CYCGRG 1 cut(s) 826
EcoICRI GAGCTC 1 cut(s) 139
EcoRII CCWGG 3 cut(s) 664, 778, 964
EcoT14I CCWWGG 2 cut(s) 267, 1111
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 267, 1111
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 7 cut(s) 158, 271, 308, 407, 698, 704, 1115
FatI CATG 7 cut(s) 154, 267, 304, 403, 694, 700, 1111
Fnu4HI GCNGC 4 cut(s) 19, 85, 234, 287
FokI GGATG 3 cut(s) 374, 618, 847
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 4 cut(s) 19, 85, 234, 287
GlaI GCGC 1 cut(s) 237
GluI GCNGC 4 cut(s) 19, 85, 234, 287
GsuI CTGGAG 2 cut(s) 125, 987
HaeIII GGCC 3 cut(s) 358, 527, 644
HapII CCGG 3 cut(s) 81, 359, 645
HgaI GACGC 2 cut(s) 44, 76
HhaI GCGC 1 cut(s) 238
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 7 cut(s) 158, 271, 308, 407, 698, 704, 1115
Hin6I GCGC 1 cut(s) 236
HinP1I GCGC 1 cut(s) 236
HindIII AAGCTT 3 cut(s) 173, 504, 673
HinfI GANTC 3 cut(s) 329, 350, 1097
HpaII CCGG 3 cut(s) 81, 359, 645
HphI GGTGA 2 cut(s) 85, 611
Hpy166II GTNNAC 3 cut(s) 808, 871, 873
Hpy188I TCNGA 2 cut(s) 136, 1119
Hpy188III TCNNGA 6 cut(s) 192, 260, 539, 797, 1042, 1086
Hpy8I GTNNAC 3 cut(s) 808, 871, 873
HpyAV CCTTC 5 cut(s) 560, 590, 596, 962, 1114
HpyCH4III ACNGT 5 cut(s) 437, 452, 488, 868, 1081
HpyCH4V TGCA 6 cut(s) 158, 558, 577, 609, 662, 808
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 1 cut(s) 254
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 7 cut(s) 158, 271, 308, 407, 698, 704, 1115
HspAI GCGC 1 cut(s) 236
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 3 cut(s) 123, 144, 1018
Lsp1109I GCAGC 2 cut(s) 220, 298
LweI GCATC 2 cut(s) 253, 596
MaeIII GTNAC 6 cut(s) 160, 223, 446, 740, 916, 1003
MboII GAAGA 7 cut(s) 68, 118, 503, 605, 953, 970, 1147
MhlI GDGCHC 5 cut(s) 141, 376, 423, 810, 891
MluCI AATT 6 cut(s) 28, 214, 366, 1125, 1143, 1151
MluI ACGCGT 1 cut(s) 36
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 1 cut(s) 1045
MseI TTAA 3 cut(s) 585, 732, 1128
MslI CAYNNNNRTG 3 cut(s) 113, 294, 699
MspI CCGG 3 cut(s) 81, 359, 645
MspR9I CCNGG 4 cut(s) 646, 666, 780, 966
MvaI CCWGG 3 cut(s) 666, 780, 966
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 1 cut(s) 24
NciI CCSGG 1 cut(s) 646
NcoI CCATGG 2 cut(s) 267, 1111
NlaIII CATG 7 cut(s) 158, 271, 308, 407, 698, 704, 1115
NlaIV GGNNCC 1 cut(s) 373
NmuCI GTSAC 3 cut(s) 160, 740, 916
NspI RCATGY 1 cut(s) 158
PciSI GCTCTTC 1 cut(s) 131
PfeI GAWTC 2 cut(s) 350, 1097
PfoI TCCNGGA 1 cut(s) 964
PkrI GCNGC 4 cut(s) 20, 86, 235, 288
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
Psp124BI GAGCTC 1 cut(s) 141
Psp6I CCWGG 3 cut(s) 664, 778, 964
PspGI CCWGG 3 cut(s) 664, 778, 964
PspN4I GGNNCC 1 cut(s) 373
PspPI GGNCC 2 cut(s) 496, 525
RsaI GTAC 4 cut(s) 41, 73, 699, 872
RsaNI GTAC 4 cut(s) 40, 72, 698, 871
RseI CAYNNNNRTG 3 cut(s) 113, 294, 699
SacI GAGCTC 1 cut(s) 141
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 3 cut(s) 585, 732, 1128
SatI GCNGC 4 cut(s) 19, 85, 234, 287
Sau96I GGNCC 2 cut(s) 496, 525
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 4 cut(s) 646, 666, 780, 966
SduI GDGCHC 5 cut(s) 141, 376, 423, 810, 891
SfaNI GCATC 2 cut(s) 253, 596
SfcI CTRYAG 1 cut(s) 64
SinI GGWCC 1 cut(s) 496
SmiMI CAYNNNNRTG 3 cut(s) 113, 294, 699
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 6 cut(s) 28, 214, 366, 1125, 1143, 1151
SsiI CCGC 3 cut(s) 19, 25, 84
SspI AATATT 2 cut(s) 845, 1092
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 4 cut(s) 644, 664, 778, 964
StyI CCWWGG 2 cut(s) 267, 1111
TaaI ACNGT 5 cut(s) 437, 452, 488, 868, 1081
TaqI TCGA 2 cut(s) 191, 219
TasI AATT 6 cut(s) 28, 214, 366, 1125, 1143, 1151
TatI WGTACW 2 cut(s) 697, 870
TauI GCSGC 2 cut(s) 21, 87
TfiI GAWTC 2 cut(s) 350, 1097
Tru1I TTAA 3 cut(s) 585, 732, 1128
Tru9I TTAA 3 cut(s) 585, 732, 1128
TscAI CASTG 4 cut(s) 165, 560, 815, 925
TseFI GTSAC 3 cut(s) 160, 740, 916
TseI GCWGC 2 cut(s) 233, 286
Tsp45I GTSAC 3 cut(s) 160, 740, 916
TspDTI ATGAA 3 cut(s) 606, 644, 683
TspGWI ACGGA 3 cut(s) 179, 488, 1028
TspRI CASTG 4 cut(s) 165, 560, 815, 925
VneI GTGCAC 1 cut(s) 806
VpaK11BI GGWCC 1 cut(s) 496
XapI RAATTY 1 cut(s) 1151
XceI RCATGY 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.