MD08G1184400.v1.1

Leucine-rich repeats, typical (most populated) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008810
Physical Location & Seq
Forward (+)
23003218 .. 23006846
3629 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1184400.v1.1.491

Sequence Viewer

Length: 2070 bp
ATGGCAAAATTGAGTTGCTTTTCTGCAATTGTTAGGGGGAAGAAGAAGGTTAAGGGAGATGAATCTACAAGAAGTGGTGAACTGCGCAAGGCAATTGAAACCCTGCACGTCAGGCTTCAACAGCAGCCTGTGAAATCGTTGGAGAGGGATGGGAAATCGAAGCCGGAAACTTTAGGAGTCCTAATACCTTATGGTGTTGAGAAGAAGTACTCTTCACCTTCTAGTGGCAATACAAGGAGCCCGAAAAGTCCTATCGGGTGTGTTGAAGCAGTAGAAGCAGCGGCTTATGAAGGGGAATACGAGCACGAAGAGAGCCCCATAGCCAGGGGAAACTCTTTTAATGATCACTCTGATCTGCAACTAGATGAAGCCAATTCAGGTGAAGTGACTCCATCAAGAAAGTTGGACTTATCCGAACCTGATTCGAGCACTCGAAGTGTTGATAGGGTTCAAAGTGGGCATCTTAGCGATCCCGGGGTTGGTAAGGCGGAGTTTTGGGCATCACCAAAGCTTACCAGATCATGCTCTAATTTGGAGACTCATAAGCAAGTGGTTAAAGAGATGTCCTCCAAGATGCCTGCTTTGAAGTCTCCGTCTTTCGAAGAACTGCAGGAAATGGCTGATAGTGTGAGGAAGGATGTCGATCCAGGCAGCCCTGGTTCTGTGTTAAGCCACTACAGTGCTGATAGAGTGATGCTGAAGAAGCATTCTTCAAGCCAAGTTCTGCCTTCGCGAAGTCGAAAATTGTGGTGGAAGCTGTTCCTTTGGAGCCACAGGAATCTGCACAGAACACGGGATGCCAAACCAAAGACACTTAGTCCCATTTCTGTGAACAAGCAAGGAGGGTACTCTTCAGATACACTCGAACCAAATCGAGCTATGCAGTTTGGCAAGACAGAATCACCACTATCATATGGTGGACAATCCTTGGACAAGGGGAAGAACATTGTAGATGACAACAAGAGCTGGGGTGGTTTTCACATTGGTGCATTGGACTTATGGCCTCAGAACCAGTGGGTTGCCTTCTCGACAGAACCCTCATCATCTTCAAGGGTGCAAGAGTGGGTGAAAGATCTCAGAATACAAACCTCCATTGAAAATAATGAAGATGACCACGAGGGAATTGTCTCCCCACAAACACCACCCACTCCTAAGACTCCAAAGTCTCCCATGACTCCTGAGACCGCTAGATCGAAAAGCGCCACTTACTTTAGCCGAGGTCTTGAAGAGGATACCTTACATGCTAATACTGTGATCCAGTCTCTAAATTCTTCCTCAACAGTGGCTCACATATCTGGTATGGGCATGAAAGCTATCCCCAACATTTCATGCTTCTGCAGCCTTCGGTCTGTCAACTTGTCGAACAACTTCATAGCCAACATTACTCCAGGGTTGCTGCCGAAGAGTCTTCACACACTCGACTTGTCCAAAAACAGGCTCAGCACCACTGAAGGACTAAGAGACTTAACCCGATTGCGAGTACTTGATCTCAGTTACAACCGAATTTCTAGAATTGGACGTGGGTTATCAAGCTGTAGTGTATTGAAGGAGTTATATCTTACCGGGAACAAGATCAGTGATGTTGAGGGCCTGCATAGGCTACTGAAGCTAACAGTTCTGGACTTGAGCTTCAACAAGATCACAACAACAAAGGCGCTGGGCCAGCTGGTGGCTAACTACAACTCACTGCAGGCTATGAATCTATTGGGGAATCCAATCCAGAACAACATCGGCGATGAGCAACTACGCAAGACAGTTTGCAGTCTCCTCCCAAAGCTAGTGTACCTGAACAAGCAGTCCATCAAGCCACAGAGGGCAAGGGGGGTAGTCACAGACAGTGTTGCCAAAGCAGCACTTGGGAACAAAGGATGGAACCCTAGAAGAAAATCCAGCAAGAAGCTTAGCCAGAGAAGCGCATCGTTTAAAAATACTCATGCCTTGCCATCGAAGAAAAGCTCATCATTTCCTACCCACGGGCACAACACCGGGGCAAGTGTTGTACAGCAAAACCTAAGCAAGACGAAGAGCATGGTCAGAGTGTCATCTGAACTCCCCTCTTCATCGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0001578 GO:0001889 GO:0001947 GO:0002009 GO:0003002 GO:0003007 GO:0003143 GO:0003341 GO:0003352 GO:0003356 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0005856 GO:0005886 GO:0005929 GO:0005930 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007275 GO:0007368 GO:0007389 GO:0007507 GO:0008150 GO:0009653 GO:0009790 GO:0009799 GO:0009855 GO:0009887 GO:0009888 GO:0009987 GO:0016020 GO:0016043 GO:0016604 GO:0016607 GO:0022607 GO:0030030 GO:0030031 GO:0030323 GO:0030324 GO:0031016 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032838 GO:0032879 GO:0032886 GO:0034622 GO:0035050 GO:0035082 GO:0035239 GO:0035295 GO:0035469 GO:0036158 GO:0036159 GO:0042995 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044451 GO:0044458 GO:0044463 GO:0044464 GO:0044782 GO:0044877 GO:0048513 GO:0048562 GO:0048565 GO:0048568 GO:0048598 GO:0048729 GO:0048731 GO:0048732 GO:0048856 GO:0050789 GO:0050794 GO:0051270 GO:0055123 GO:0060271 GO:0060429 GO:0060541 GO:0060562 GO:0060632 GO:0060972 GO:0061008 GO:0061371 GO:0065003 GO:0065007 GO:0070013 GO:0070286 GO:0070840 GO:0070925 GO:0071840 GO:0071907 GO:0071910 GO:0071944 GO:0072359 GO:0097014 GO:0099568 GO:0120025 GO:0120031 GO:0120036 GO:0120038
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_6 LRR_8

Protein Analysis

690

Amino Acids

75.77

Weight (kDa)

9.67

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 447 - 502 2.6e-08 Leucine rich repeat
LRR_4 PF12799 468 - 505 3.1e-07 Leucine Rich repeats (2 copies)
LRR_8 PF13855 469 - 525 1.6e-08 Leucine rich repeat
LRR_9 PF14580 469 - 552 2.8e-08 Leucine-rich repeat
LRR_8 PF13855 491 - 547 5.2e-09 Leucine rich repeat
LRR_4 PF12799 515 - 552 6.6e-07 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1162
Acc16I TGCGCA 1 cut(s) 86
AccB7I CCANNNNNTGG 1 cut(s) 1669
AccII CGCG 1 cut(s) 733
AciI CCGC 3 cut(s) 281, 488, 1185
AclWI GGATC 3 cut(s) 464, 638, 1249
AcsI RAATTY 2 cut(s) 1267, 1503
AcuI CTGAAG 4 cut(s) 719, 837, 1470, 1625
AfaI GTAC 5 cut(s) 209, 848, 1482, 1784, 2001
AfiI CCNNNNNNNGG 6 cut(s) 224, 324, 479, 1434, 1669, 1973
AhdI GACNNNNNGTC 1 cut(s) 816
AjiI CACGTC 2 cut(s) 109, 1520
AjnI CCWGG 4 cut(s) 323, 646, 655, 1387
AleI CACNNNNGTG 2 cut(s) 678, 984
AloI GAACNNNNNNTCC 2 cut(s) 1781, 1813
Alw21I GWGCWC 2 cut(s) 306, 431
Alw26I GTCTC 8 cut(s) 530, 594, 1132, 1170, 1175, 1266, 1455, 1769
AlwI GGATC 3 cut(s) 464, 638, 1249
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 3 cut(s) 1001, 1588, 1660
ApeKI GCWGC 6 cut(s) 124, 278, 651, 1338, 1396, 1850
ApoI RAATTY 2 cut(s) 1267, 1503
Asp700I GAANNNNTTC 2 cut(s) 758, 1367
AspLEI GCGC 4 cut(s) 87, 1202, 1657, 1916
AspS9I GGNCC 2 cut(s) 1588, 1660
AsuC2I CCSGG 4 cut(s) 474, 475, 1564, 1987
AsuHPI GGTGA 6 cut(s) 89, 207, 392, 495, 894, 1078
AsuII TTCGAA 1 cut(s) 600
AvaI CYCGRG 1 cut(s) 473
BaeGI GKGCMC 1 cut(s) 1980
BanII GRGCYC 2 cut(s) 242, 317
BauI CACGAG 1 cut(s) 1115
BbsI GAAGAC 1 cut(s) 1400
Bbv12I GWGCWC 2 cut(s) 306, 431
BbvI GCAGC 6 cut(s) 136, 290, 663, 1350, 1383, 1862
BccI CCATC 5 cut(s) 143, 400, 1808, 1863, 1951
BciT130I CCWGG 4 cut(s) 325, 648, 657, 1389
BciVI GTATCC 1 cut(s) 1225
BclI TGATCA 1 cut(s) 343
BcnI CCSGG 4 cut(s) 474, 475, 1564, 1987
BcoDI GTCTC 8 cut(s) 530, 594, 1132, 1170, 1175, 1266, 1455, 1769
BfaI CTAG 6 cut(s) 222, 362, 1188, 1509, 1778, 1878
BfmI CTRYAG 5 cut(s) 608, 676, 1336, 1534, 1688
BfoI RGCGCY 2 cut(s) 1203, 1658
BfuI GTATCC 1 cut(s) 1225
BglII AGATCT 1 cut(s) 1072
BisI GCNGC 7 cut(s) 125, 279, 282, 652, 1339, 1397, 1851
BlpI GCTNAGC 2 cut(s) 1439, 1901
BlsI GCNGC 7 cut(s) 126, 280, 283, 653, 1340, 1398, 1852
BmcAI AGTACT 2 cut(s) 209, 1482
Bme1390I CCNGG 8 cut(s) 325, 474, 475, 648, 657, 1389, 1564, 1987
BmeRI GACNNNNNGTC 1 cut(s) 816
BmeT110I CYCGRG 1 cut(s) 473
BmgBI CACGTC 2 cut(s) 109, 1520
BmgT120I GGNCC 2 cut(s) 1588, 1660
BmiI GGNNCC 3 cut(s) 239, 770, 1874
BmrFI CCNGG 8 cut(s) 325, 474, 475, 648, 657, 1389, 1564, 1987
BmsI GCATC 6 cut(s) 469, 509, 564, 684, 787, 1925
BpiI GAAGAC 1 cut(s) 1400
BplI GAGNNNNNCTC 2 cut(s) 551, 583
BpmI CTGGAG 1 cut(s) 1371
Bpu10I CCTNAGC 1 cut(s) 2012
Bpu1102I GCTNAGC 2 cut(s) 1439, 1901
Bpu14I TTCGAA 1 cut(s) 600
BpuEI CTTGAG 1 cut(s) 1645
BpuMI CCSGG 4 cut(s) 474, 475, 1564, 1987
BsaBI GATNNNNATC 1 cut(s) 642
BsaI GGTCTC 1 cut(s) 1175
BsaJI CCNNGG 9 cut(s) 324, 473, 474, 655, 927, 1216, 1388, 1972, 1986
Bsc4I CCNNNNNNNGG 6 cut(s) 224, 324, 479, 1434, 1669, 1973
Bse1I ACTGG 2 cut(s) 1012, 1258
Bse8I GATNNNNATC 1 cut(s) 642
BseBI CCWGG 4 cut(s) 325, 648, 657, 1389
BseDI CCNNGG 9 cut(s) 324, 473, 474, 655, 927, 1216, 1388, 1972, 1986
BseGI GGATG 4 cut(s) 154, 643, 802, 1874
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 6 cut(s) 224, 324, 479, 1434, 1669, 1973
BseMII CTCAG 5 cut(s) 1019, 1090, 1170, 1453, 1504
BseNI ACTGG 2 cut(s) 1012, 1258
BseRI GAGGAG 1 cut(s) 1757
BseSI GKGCMC 1 cut(s) 1980
BseXI GCAGC 6 cut(s) 136, 290, 663, 1350, 1383, 1862
BseYI CCCAGC 2 cut(s) 966, 1657
BsgI GTGCAG 2 cut(s) 89, 767
Bsh1236I CGCG 1 cut(s) 733
BshFI GGCC 3 cut(s) 1003, 1590, 1662
BsiHKAI GWGCWC 2 cut(s) 306, 431
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 4 cut(s) 164, 474, 1563, 1986
BslFI GGGAC 1 cut(s) 804
BslI CCNNNNNNNGG 6 cut(s) 224, 324, 479, 1434, 1669, 1973
BsmAI GTCTC 8 cut(s) 530, 594, 1132, 1170, 1175, 1266, 1455, 1769
BsmFI GGGAC 1 cut(s) 804
BsmI GAATGC 1 cut(s) 706
BsnI GGCC 3 cut(s) 1003, 1590, 1662
Bso31I GGTCTC 1 cut(s) 1175
BsoBI CYCGRG 1 cut(s) 473
Bsp119I TTCGAA 1 cut(s) 600
Bsp1286I GDGCHC 5 cut(s) 242, 306, 317, 431, 1980
Bsp1407I TGTACA 1 cut(s) 1999
Bsp1720I GCTNAGC 2 cut(s) 1439, 1901
Bsp68I TCGCGA 1 cut(s) 733
BspACI CCGC 3 cut(s) 281, 488, 1185
BspANI GGCC 3 cut(s) 1003, 1590, 1662
BspCNI CTCAG 5 cut(s) 1018, 1089, 1171, 1452, 1503
BspFNI CGCG 1 cut(s) 733
BspLI GGNNCC 3 cut(s) 239, 770, 1874
BspMAI CTGCAG 3 cut(s) 612, 1340, 1692
BspPI GGATC 3 cut(s) 464, 638, 1249
BspQI GCTCTTC 1 cut(s) 2018
BspT104I TTCGAA 1 cut(s) 600
BspTNI GGTCTC 1 cut(s) 1175
BsrGI TGTACA 1 cut(s) 1999
BsrI ACTGG 2 cut(s) 1012, 1258
BssECI CCNNGG 9 cut(s) 324, 473, 474, 655, 927, 1216, 1388, 1972, 1986
BssSI CACGAG 1 cut(s) 1115
BssT1I CCWWGG 1 cut(s) 927
Bst2BI CACGAG 1 cut(s) 1115
Bst2UI CCWGG 4 cut(s) 325, 648, 657, 1389
Bst4CI ACNGT 6 cut(s) 680, 1252, 1282, 1615, 1756, 1838
Bst6I CTCTTC 7 cut(s) 217, 303, 856, 1221, 1397, 2018, 2062
BstAUI TGTACA 1 cut(s) 1999
BstBI TTCGAA 1 cut(s) 600
BstC8I GCNNGC 4 cut(s) 579, 1592, 1664, 1692
BstDSI CCRYGG 1 cut(s) 1972
BstF5I GGATG 4 cut(s) 154, 643, 802, 1874
BstFNI CGCG 1 cut(s) 733
BstH2I RGCGCY 2 cut(s) 1203, 1658
BstHHI GCGC 4 cut(s) 87, 1202, 1657, 1916
BstMAI GTCTC 8 cut(s) 530, 594, 1132, 1170, 1175, 1266, 1455, 1769
BstMWI GCNNNNNNNGC 9 cut(s) 112, 121, 275, 703, 1338, 1606, 1663, 1850, 1911
BstNI CCWGG 4 cut(s) 325, 648, 657, 1389
BstNSI RCATGY 1 cut(s) 1244
BstSCI CCNGG 8 cut(s) 323, 472, 473, 646, 655, 1387, 1562, 1985
BstSFI CTRYAG 5 cut(s) 608, 676, 1336, 1534, 1688
BstSLI GKGCMC 1 cut(s) 1980
BstUI CGCG 1 cut(s) 733
BstV1I GCAGC 6 cut(s) 136, 290, 663, 1350, 1383, 1862
BstV2I GAAGAC 1 cut(s) 1400
BstX2I RGATCY 1 cut(s) 1072
BstYI RGATCY 1 cut(s) 1072
BsuI GTATCC 1 cut(s) 1225
BsuRI GGCC 3 cut(s) 1003, 1590, 1662
BtgI CCRYGG 1 cut(s) 1972
BtgZI GCGATG 2 cut(s) 1749, 2046
BtrI CACGTC 2 cut(s) 109, 1520
BtsCI GGATG 4 cut(s) 154, 643, 802, 1874
BtsI GCAGTG 1 cut(s) 1685
BtsIMutI CAGTG 7 cut(s) 685, 1019, 1287, 1446, 1582, 1685, 1843
BtuMI TCGCGA 1 cut(s) 733
Cac8I GCNNGC 4 cut(s) 579, 1592, 1664, 1692
CfoI GCGC 4 cut(s) 87, 1202, 1657, 1916
Cfr13I GGNCC 2 cut(s) 1588, 1660
Cfr9I CCCGGG 1 cut(s) 473
Csp6I GTAC 5 cut(s) 208, 847, 1481, 1783, 2000
CviAII CATG 7 cut(s) 522, 1171, 1241, 1306, 1329, 1934, 2029
CviQI GTAC 5 cut(s) 208, 847, 1481, 1783, 2000
DraI TTTAAA 1 cut(s) 1924
DrdI GACNNNNNNGTC 1 cut(s) 1162
DriI GACNNNNNGTC 1 cut(s) 816
DseDI GACNNNNNNGTC 1 cut(s) 1162
Eam1104I CTCTTC 7 cut(s) 217, 303, 856, 1221, 1397, 2018, 2062
Eam1105I GACNNNNNGTC 1 cut(s) 816
EarI CTCTTC 7 cut(s) 217, 303, 856, 1221, 1397, 2018, 2062
EciI GGCGGA 1 cut(s) 503
Eco130I CCWWGG 1 cut(s) 927
Eco24I GRGCYC 2 cut(s) 242, 317
Eco31I GGTCTC 1 cut(s) 1175
Eco57I CTGAAG 4 cut(s) 719, 837, 1470, 1625
Eco88I CYCGRG 1 cut(s) 473
EcoO109I RGGNCCY 1 cut(s) 1588
EcoRII CCWGG 4 cut(s) 323, 646, 655, 1387
EcoT14I CCWWGG 1 cut(s) 927
EcoT38I GRGCYC 2 cut(s) 242, 317
ErhI CCWWGG 1 cut(s) 927
FaeI CATG 7 cut(s) 525, 1174, 1244, 1309, 1332, 1937, 2032
FalI AAGNNNNNCTT 6 cut(s) 392, 424, 1189, 1221, 1839, 1871
FaqI GGGAC 1 cut(s) 804
FatI CATG 7 cut(s) 521, 1170, 1240, 1305, 1328, 1933, 2028
FauNDI CATATG 1 cut(s) 913
FbaI TGATCA 1 cut(s) 343
Fnu4HI GCNGC 7 cut(s) 125, 279, 282, 652, 1339, 1397, 1851
FokI GGATG 4 cut(s) 161, 650, 809, 1881
FriOI GRGCYC 2 cut(s) 242, 317
Fsp4HI GCNGC 7 cut(s) 125, 279, 282, 652, 1339, 1397, 1851
FspBI CTAG 6 cut(s) 222, 362, 1188, 1509, 1778, 1878
FspI TGCGCA 1 cut(s) 86
GlaI GCGC 4 cut(s) 86, 1201, 1656, 1915
GluI GCNGC 7 cut(s) 125, 279, 282, 652, 1339, 1397, 1851
GsaI CCCAGC 2 cut(s) 970, 1661
GsuI CTGGAG 1 cut(s) 1371
HaeII RGCGCY 2 cut(s) 1203, 1658
HaeIII GGCC 3 cut(s) 1003, 1590, 1662
HapII CCGG 4 cut(s) 164, 474, 1563, 1986
HhaI GCGC 4 cut(s) 87, 1202, 1657, 1916
Hin1II CATG 7 cut(s) 525, 1174, 1244, 1309, 1332, 1937, 2032
Hin6I GCGC 4 cut(s) 85, 1200, 1655, 1914
HinP1I GCGC 4 cut(s) 85, 1200, 1655, 1914
HincII GTYRAC 1 cut(s) 1354
HindII GTYRAC 1 cut(s) 1354
HindIII AAGCTT 2 cut(s) 509, 1898
HpaII CCGG 4 cut(s) 164, 474, 1563, 1986
HphI GGTGA 6 cut(s) 89, 207, 392, 495, 894, 1078
Hpy166II GTNNAC 5 cut(s) 80, 832, 920, 1354, 1783
Hpy188I TCNGA 7 cut(s) 352, 415, 856, 1008, 1079, 2036, 2047
Hpy188III TCNNGA 8 cut(s) 396, 732, 1027, 1178, 1223, 1509, 1619, 1720
Hpy8I GTNNAC 5 cut(s) 80, 832, 920, 1354, 1783
HpyAV CCTTC 9 cut(s) 40, 228, 284, 628, 738, 1033, 1352, 1445, 1540
HpyCH4III ACNGT 6 cut(s) 680, 1252, 1282, 1615, 1756, 1838
HpyCH4IV ACGT 2 cut(s) 108, 1519
HpyF10VI GCNNNNNNNGC 9 cut(s) 112, 121, 275, 703, 1338, 1606, 1663, 1850, 1911
HpySE526I ACGT 2 cut(s) 108, 1519
Hsp92II CATG 7 cut(s) 525, 1174, 1244, 1309, 1332, 1937, 2032
HspAI GCGC 4 cut(s) 85, 1200, 1655, 1914
Ksp22I TGATCA 1 cut(s) 343
LguI GCTCTTC 1 cut(s) 2018
LmnI GCTCC 2 cut(s) 237, 768
Lsp1109I GCAGC 6 cut(s) 136, 290, 663, 1350, 1383, 1862
LweI GCATC 6 cut(s) 469, 509, 564, 684, 787, 1925
MaeI CTAG 6 cut(s) 222, 362, 1188, 1509, 1778, 1878
MaeII ACGT 2 cut(s) 108, 1519
MaeIII GTNAC 3 cut(s) 385, 1493, 1828
MfeI CAATTG 2 cut(s) 27, 93
MflI RGATCY 1 cut(s) 1072
MhlI GDGCHC 5 cut(s) 242, 306, 317, 431, 1980
MlyI GAGTC 6 cut(s) 186, 382, 532, 1150, 1168, 1414
MmeI TCCRAC 2 cut(s) 120, 384
MroXI GAANNNNTTC 2 cut(s) 758, 1367
MseI TTAA 6 cut(s) 51, 339, 555, 668, 1466, 1923
MslI CAYNNNNRTG 3 cut(s) 678, 827, 984
MspA1I CMGCKG 2 cut(s) 281, 1666
MspI CCGG 4 cut(s) 164, 474, 1563, 1986
MspR9I CCNGG 8 cut(s) 325, 474, 475, 648, 657, 1389, 1564, 1987
MunI CAATTG 2 cut(s) 27, 93
Mva1269I GAATGC 1 cut(s) 706
MvaI CCWGG 4 cut(s) 325, 648, 657, 1389
MvnI CGCG 1 cut(s) 733
MwoI GCNNNNNNNGC 9 cut(s) 112, 121, 275, 703, 1338, 1606, 1663, 1850, 1911
NciI CCSGG 4 cut(s) 474, 475, 1564, 1987
NdeI CATATG 1 cut(s) 913
NlaIII CATG 7 cut(s) 525, 1174, 1244, 1309, 1332, 1937, 2032
NlaIV GGNNCC 3 cut(s) 239, 770, 1874
NmeAIII GCCGAG 1 cut(s) 1241
NmuCI GTSAC 2 cut(s) 385, 1828
NruI TCGCGA 1 cut(s) 733
NsbI TGCGCA 1 cut(s) 86
NspI RCATGY 1 cut(s) 1244
NspV TTCGAA 1 cut(s) 600
OliI CACNNNNGTG 2 cut(s) 678, 984
PciSI GCTCTTC 1 cut(s) 2018
PctI GAATGC 1 cut(s) 706
PdmI GAANNNNTTC 2 cut(s) 758, 1367
PfeI GAWTC 6 cut(s) 62, 422, 778, 899, 1699, 1711
PflMI CCANNNNNTGG 1 cut(s) 1669
PkrI GCNGC 7 cut(s) 126, 280, 283, 653, 1340, 1398, 1852
PleI GAGTC 6 cut(s) 185, 382, 532, 1150, 1168, 1413
PpsI GAGTC 6 cut(s) 185, 382, 532, 1150, 1168, 1413
Psp6I CCWGG 4 cut(s) 323, 646, 655, 1387
PspFI CCCAGC 2 cut(s) 966, 1657
PspGI CCWGG 4 cut(s) 323, 646, 655, 1387
PspN4I GGNNCC 3 cut(s) 239, 770, 1874
PspPI GGNCC 2 cut(s) 1588, 1660
PstI CTGCAG 3 cut(s) 612, 1340, 1692
PsuI RGATCY 1 cut(s) 1072
PvuII CAGCTG 1 cut(s) 1666
RruI TCGCGA 1 cut(s) 733
RsaI GTAC 5 cut(s) 209, 848, 1482, 1784, 2001
RsaNI GTAC 5 cut(s) 208, 847, 1481, 1783, 2000
RseI CAYNNNNRTG 3 cut(s) 678, 827, 984
SapI GCTCTTC 1 cut(s) 2018
SaqAI TTAA 6 cut(s) 51, 339, 555, 668, 1466, 1923
SatI GCNGC 7 cut(s) 125, 279, 282, 652, 1339, 1397, 1851
Sau96I GGNCC 2 cut(s) 1588, 1660
ScaI AGTACT 2 cut(s) 209, 1482
SchI GAGTC 6 cut(s) 186, 382, 532, 1150, 1168, 1414
ScrFI CCNGG 8 cut(s) 325, 474, 475, 648, 657, 1389, 1564, 1987
SduI GDGCHC 5 cut(s) 242, 306, 317, 431, 1980
SfaNI GCATC 6 cut(s) 469, 509, 564, 684, 787, 1925
SfcI CTRYAG 5 cut(s) 608, 676, 1336, 1534, 1688
SfuI TTCGAA 1 cut(s) 600
SmaI CCCGGG 1 cut(s) 475
SmiMI CAYNNNNRTG 3 cut(s) 678, 827, 984
SmlI CTYRAG 1 cut(s) 1624
SmoI CTYRAG 1 cut(s) 1624
SsiI CCGC 3 cut(s) 281, 488, 1185
SspMI CTAG 6 cut(s) 222, 362, 1188, 1509, 1778, 1878
StyD4I CCNGG 8 cut(s) 323, 472, 473, 646, 655, 1387, 1562, 1985
StyI CCWWGG 1 cut(s) 927
TaaI ACNGT 6 cut(s) 680, 1252, 1282, 1615, 1756, 1838
TaiI ACGT 2 cut(s) 111, 1522
TaqII GACCGA 1 cut(s) 1335
TatI WGTACW 3 cut(s) 207, 1480, 1999
TauI GCSGC 1 cut(s) 284
TfiI GAWTC 6 cut(s) 62, 422, 778, 899, 1699, 1711
Tru1I TTAA 6 cut(s) 51, 339, 555, 668, 1466, 1923
Tru9I TTAA 6 cut(s) 51, 339, 555, 668, 1466, 1923
TscAI CASTG 7 cut(s) 685, 1019, 1287, 1453, 1582, 1692, 1843
TseFI GTSAC 2 cut(s) 385, 1828
TseI GCWGC 6 cut(s) 124, 278, 651, 1338, 1396, 1850
Tsp45I GTSAC 2 cut(s) 385, 1828
TspDTI ATGAA 9 cut(s) 75, 303, 381, 1119, 1317, 1322, 1360, 1712, 2049
TspGWI ACGGA 1 cut(s) 582
TspMI CCCGGG 1 cut(s) 473
TspRI CASTG 7 cut(s) 685, 1019, 1287, 1453, 1582, 1692, 1843
Van91I CCANNNNNTGG 1 cut(s) 1669
XapI RAATTY 2 cut(s) 1267, 1503
XbaI TCTAGA 1 cut(s) 1508
XceI RCATGY 1 cut(s) 1244
XmaI CCCGGG 1 cut(s) 473
XmnI GAANNNNTTC 2 cut(s) 758, 1367
XspI CTAG 6 cut(s) 222, 362, 1188, 1509, 1778, 1878
ZrmI AGTACT 2 cut(s) 209, 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.