pycom15g33260

Leucine-rich repeats, typical (most populated) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008810
Physical Location & Seq
Forward (+)
32568552 .. 32570851
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g33260.1

Sequence Viewer

Length: 1989 bp
ATGGCAAAATTCAGTTGCTTTTCTTTAATTGGTAGGAGGAAGAAGAAGGTTAAGGGAGATGATGAATCTACAAGAAGTGGTGAAGTGGACAAGGCAATTAGAACCCTGCAAGTCAGGCTTCAACAGCAGCCTCTGAAACCGTTGGAGATTGATGGGAAATCGAAGCCGGCAACTTTCGGAGTCCTAATACCTTATGGTGTTGAGAAGAAGTACTCTTCCCCTCCTAGTGGCAATACAAGGAGCCCGAAAAGTCCTATCAGGTGCGTTGAAGCAGCGGCTTGTGAAGGGGAATATGAGCACAAGGAGAGCCCCTTAGCCAAGGGAAGCTCCTCTAATGATCACTCTGATCTGCAACCCAATGAAGCCAATTCAGGTGACGTGACTCCATCAAGGAAGTTGGATTTATCCGAACCTGATTCGAGCTCTCGAGGGGTTCAAAGCGGGCATTTTAGTGATCCCGGGGTTGGTAAGGCCGAGTTTTGGGCATCACCAAAGCTTACCAGATCATGCTCTAATTTGGAGACTCATAAGAAAGTTGTTAAAAAGATGACCTCTAAGATGCCTGCTTCGAAGTCTCTGTCTCTCGAAGAACGGCAGGAAATGGCTGATAGTGTGAGGAAGGATGTTGATCCAGGCAGCCCTAGTTCTGTGTCAAGCCACTACAGTGCTGATAGAGTGATGCTGACGAAGCATTCTTCAAGCCAAGTTCTGCCTTCTCGAAGTCGAAAATTGTGGTGGAAGTTGTTCCTTTGGAGCCACAGGAACCTGCACAGAACACGGGATGCAAAACCAAATACACTTGGTCCCATTTCAGCGAACAAGCAAGGAGGGTACTCTTCAGACACACTCGAACCAAATCTAGCTATGCAGTTTGGCAAGACGGAATCACCACTATCATTTACTGGAGAATTGTTGGACAAGGGGAAGAACATTGTAGATGAAAACAAGAGCTGGGATGGTTTTCACATTGGTGCGTCGGCCTTATGGCCTCAGAACCAATGGGTTGCTTTCTCGACAGAAGCCTCATCATCTTCAAGAGTGCAAGACTGGGTGAAAGATCTCAGAATACAAACCTCCGTTGAAAAGAATGAAGATGACAATGAGGGAATCGTCTCCCCACGGACACCCCCCACTCCTGAGACTCCAAAGTCTCCTGTGACTCCTGCGACTGCTAGATCGAAAAGCGCCACTTACTTCAGCCGAGGCCTTGAAGAGGATACCTTACATGCTAATACTGTGATCCAGTCTCTAAATTCTTGCTCAACAGTGGCTCACATATCTGGTATGGGCATGAAAGCCATCCCCAACATTTCATGCTTCTGCAGTCTTCGATCTGTTAACTTGTCGAACAACTTCATAGCCCACATTACTCCGGGGTCGCTGCCGAAGAGTCTTCACACGCTCAACTTGTCCAAAAACAAGCTCAGCGCCATTGAAGGACTAAGAGACTTAACCCGATTGCGAGTACTTGATCTCAGTTACAACCGAATATCTAGAATCGGACGTGGATTATCGAGCTGTAATGTATTGAAGGAGCTCTATCTTTCCGGGAACAAGATTAGCGATGTAGAGGGTCTGCATAGGCTACTGAAGCTGACAGTTCTGGACTTGAGCTTCAACAAGATTACAACAACAAGGGCACTGGGCCAGCTGGTGGCTAACTACAACTCACTGCAGGCTCTAAACCTATTAGGGAATACAATCCAGACCAACATCGGCGATGAGCAGCTACGCAAGACAGTTTGTAGTCTCCTCCCAAAGCTAGTGTACCTGAACAAGCAGTCCATCAAGCCACAAAGAGCAAGGGAGGTACTCACCGACAGCGTAGCCAAAGCTGCACTTGGAAACAAAGGGTGGAACCCTAGAAGAAAAGCCAGCAAGAAGCTTAGCCAAAGAAGTGCATCGTTTAAAAAAACTCATGCCTTGCCATCGAAGAAAAGCTCATCATTTCCTACCCACGGGCACAAGAGAGGTGCAAGTGTTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0001578 GO:0001889 GO:0001947 GO:0002009 GO:0003002 GO:0003007 GO:0003143 GO:0003341 GO:0003352 GO:0003356 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0005856 GO:0005886 GO:0005929 GO:0005930 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007275 GO:0007368 GO:0007389 GO:0007507 GO:0008150 GO:0009653 GO:0009790 GO:0009799 GO:0009855 GO:0009887 GO:0009888 GO:0009987 GO:0016020 GO:0016043 GO:0016604 GO:0016607 GO:0022607 GO:0030030 GO:0030031 GO:0030323 GO:0030324 GO:0031016 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032838 GO:0032879 GO:0032886 GO:0034622 GO:0035050 GO:0035082 GO:0035239 GO:0035295 GO:0035469 GO:0036158 GO:0036159 GO:0042995 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044451 GO:0044458 GO:0044463 GO:0044464 GO:0044782 GO:0044877 GO:0048513 GO:0048562 GO:0048565 GO:0048568 GO:0048598 GO:0048729 GO:0048731 GO:0048732 GO:0048856 GO:0050789 GO:0050794 GO:0051270 GO:0055123 GO:0060271 GO:0060429 GO:0060541 GO:0060562 GO:0060632 GO:0060972 GO:0061008 GO:0061371 GO:0065003 GO:0065007 GO:0070013 GO:0070286 GO:0070840 GO:0070925 GO:0071840 GO:0071907 GO:0071910 GO:0071944 GO:0072359 GO:0097014 GO:0099568 GO:0120025 GO:0120031 GO:0120036 GO:0120038
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_6 LRR_8

Protein Analysis

663

Amino Acids

72.69

Weight (kDa)

9.81

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 442 - 497 6.7e-10 Leucine rich repeat
LRR_9 PF14580 462 - 547 2.4e-07 Leucine-rich repeat
LRR_4 PF12799 463 - 500 9.7e-08 Leucine Rich repeats (2 copies)
LRR_8 PF13855 482 - 520 5.3e-07 Leucine rich repeat
LRR_4 PF12799 510 - 546 1e-07 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1147
Acc36I ACCTGC 1 cut(s) 774
AccB7I CCANNNNNTGG 1 cut(s) 1654
AciI CCGC 2 cut(s) 275, 441
AclWI GGATC 3 cut(s) 449, 623, 1234
AcsI RAATTY 2 cut(s) 8, 1252
AcuI CTGAAG 3 cut(s) 822, 1180, 1610
AfaI GTAC 5 cut(s) 212, 833, 1467, 1769, 1812
AfiI CCNNNNNNNGG 6 cut(s) 227, 464, 480, 1213, 1654, 1958
AjiI CACGTC 2 cut(s) 379, 1505
AjnI CCWGG 1 cut(s) 631
AleI CACNNNNGTG 2 cut(s) 663, 969
AloI GAACNNNNNNTCC 2 cut(s) 1766, 1798
Alw21I GWGCWC 3 cut(s) 300, 425, 1539
Alw26I GTCTC 9 cut(s) 515, 579, 585, 1117, 1133, 1155, 1251, 1440, 1754
AlwI GGATC 3 cut(s) 449, 623, 1234
AlwNI CAGNNNCTG 1 cut(s) 133
Ama87I CYCGRG 2 cut(s) 426, 458
AoxI GGCC 5 cut(s) 471, 978, 986, 1204, 1645
ApeKI GCWGC 6 cut(s) 127, 272, 636, 1381, 1726, 1835
ApoI RAATTY 2 cut(s) 8, 1252
ArsI GACNNNNNNTTYG 2 cut(s) 563, 595
Asp700I GAANNNNTTC 2 cut(s) 743, 1352
AspLEI GCGC 2 cut(s) 1187, 1430
AspS9I GGNCC 2 cut(s) 803, 1645
AsuC2I CCSGG 4 cut(s) 459, 460, 1374, 1549
AsuHPI GGTGA 6 cut(s) 92, 386, 480, 879, 1063, 1807
AsuII TTCGAA 1 cut(s) 569
AvaI CYCGRG 2 cut(s) 426, 458
AvaII GGWCC 1 cut(s) 803
BaeGI GKGCMC 2 cut(s) 1642, 1965
BanII GRGCYC 4 cut(s) 245, 311, 425, 1539
BbsI GAAGAC 2 cut(s) 1319, 1385
Bbv12I GWGCWC 3 cut(s) 300, 425, 1539
BbvI GCAGC 6 cut(s) 139, 284, 648, 1368, 1738, 1822
BccI CCATC 6 cut(s) 146, 394, 950, 1307, 1793, 1936
BceAI ACGGC 1 cut(s) 608
BciT130I CCWGG 1 cut(s) 633
BciVI GTATCC 1 cut(s) 1210
BclI TGATCA 1 cut(s) 337
BcnI CCSGG 4 cut(s) 459, 460, 1374, 1549
BcoDI GTCTC 9 cut(s) 515, 579, 585, 1117, 1133, 1155, 1251, 1440, 1754
BfaI CTAG 7 cut(s) 225, 642, 860, 1173, 1494, 1763, 1863
BfmI CTRYAG 3 cut(s) 661, 1321, 1673
BfoI RGCGCY 2 cut(s) 1188, 1431
BfuAI ACCTGC 1 cut(s) 774
BfuI GTATCC 1 cut(s) 1210
BglII AGATCT 1 cut(s) 1057
BisI GCNGC 7 cut(s) 128, 273, 276, 637, 1382, 1727, 1836
BlpI GCTNAGC 2 cut(s) 1424, 1886
BlsI GCNGC 7 cut(s) 129, 274, 277, 638, 1383, 1728, 1837
BmcAI AGTACT 2 cut(s) 212, 1467
Bme1390I CCNGG 5 cut(s) 459, 460, 633, 1374, 1549
Bme18I GGWCC 1 cut(s) 803
BmeT110I CYCGRG 2 cut(s) 426, 458
BmgBI CACGTC 2 cut(s) 379, 1505
BmgT120I GGNCC 2 cut(s) 803, 1645
BmiI GGNNCC 5 cut(s) 242, 755, 764, 805, 1859
BmrFI CCNGG 5 cut(s) 459, 460, 633, 1374, 1549
BmrI ACTGGG 2 cut(s) 1057, 1652
BmsI GCATC 5 cut(s) 494, 549, 669, 772, 1910
BmuI ACTGGG 2 cut(s) 1057, 1652
BpiI GAAGAC 2 cut(s) 1319, 1385
BpmI CTGGAG 1 cut(s) 924
Bpu10I CCTNAGC 1 cut(s) 313
Bpu1102I GCTNAGC 2 cut(s) 1424, 1886
Bpu14I TTCGAA 1 cut(s) 569
BpuEI CTTGAG 1 cut(s) 1630
BpuMI CCSGG 4 cut(s) 459, 460, 1374, 1549
BsaBI GATNNNNATC 1 cut(s) 627
BsaJI CCNNGG 7 cut(s) 318, 458, 459, 1118, 1201, 1373, 1957
Bsc4I CCNNNNNNNGG 6 cut(s) 227, 464, 480, 1213, 1654, 1958
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 4 cut(s) 907, 1052, 1243, 1647
Bse8I GATNNNNATC 1 cut(s) 627
BseBI CCWGG 1 cut(s) 633
BseDI CCNNGG 7 cut(s) 318, 458, 459, 1118, 1201, 1373, 1957
BseGI GGATG 4 cut(s) 628, 787, 961, 1299
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 6 cut(s) 227, 464, 480, 1213, 1654, 1958
BseMII CTCAG 5 cut(s) 1004, 1075, 1128, 1438, 1489
BseNI ACTGG 4 cut(s) 907, 1052, 1243, 1647
BseRI GAGGAG 2 cut(s) 319, 1742
BseSI GKGCMC 2 cut(s) 1642, 1965
BseXI GCAGC 6 cut(s) 139, 284, 648, 1368, 1738, 1822
BseYI CCCAGC 1 cut(s) 951
BsgI GTGCAG 2 cut(s) 752, 1821
BshFI GGCC 5 cut(s) 473, 980, 988, 1206, 1647
BsiHKAI GWGCWC 3 cut(s) 300, 425, 1539
BsiHKCI CYCGRG 2 cut(s) 426, 458
BsiSI CCGG 4 cut(s) 167, 459, 1373, 1548
BslFI GGGAC 1 cut(s) 789
BslI CCNNNNNNNGG 6 cut(s) 227, 464, 480, 1213, 1654, 1958
BsmAI GTCTC 9 cut(s) 515, 579, 585, 1117, 1133, 1155, 1251, 1440, 1754
BsmBI CGTCTC 1 cut(s) 1117
BsmFI GGGAC 1 cut(s) 789
BsmI GAATGC 1 cut(s) 691
BsnI GGCC 5 cut(s) 473, 980, 988, 1206, 1647
BsoBI CYCGRG 2 cut(s) 426, 458
Bsp119I TTCGAA 1 cut(s) 569
Bsp1286I GDGCHC 7 cut(s) 245, 300, 311, 425, 1539, 1642, 1965
Bsp1720I GCTNAGC 2 cut(s) 1424, 1886
BspACI CCGC 2 cut(s) 275, 441
BspANI GGCC 5 cut(s) 473, 980, 988, 1206, 1647
BspCNI CTCAG 5 cut(s) 1003, 1074, 1129, 1437, 1488
BspLI GGNNCC 5 cut(s) 242, 755, 764, 805, 1859
BspMAI CTGCAG 2 cut(s) 1325, 1677
BspMI ACCTGC 1 cut(s) 774
BspPI GGATC 3 cut(s) 449, 623, 1234
BspT104I TTCGAA 1 cut(s) 569
BsrFI RCCGGY 1 cut(s) 166
BsrI ACTGG 4 cut(s) 907, 1052, 1243, 1647
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 7 cut(s) 318, 458, 459, 1118, 1201, 1373, 1957
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 6 cut(s) 141, 665, 1237, 1267, 1600, 1741
Bst6I CTCTTC 4 cut(s) 220, 841, 1206, 1382
BstBI TTCGAA 1 cut(s) 569
BstC8I GCNNGC 6 cut(s) 168, 443, 564, 1649, 1677, 1876
BstDEI CTNAG 9 cut(s) 313, 555, 990, 1061, 1137, 1424, 1442, 1475, 1886
BstDSI CCRYGG 2 cut(s) 1118, 1957
BstENI CCTNNNNNAGG 1 cut(s) 1211
BstF5I GGATG 4 cut(s) 628, 787, 961, 1299
BstH2I RGCGCY 2 cut(s) 1188, 1431
BstHHI GCGC 2 cut(s) 1187, 1430
BstMAI GTCTC 9 cut(s) 515, 579, 585, 1117, 1133, 1155, 1251, 1440, 1754
BstMWI GCNNNNNNNGC 5 cut(s) 115, 124, 688, 1591, 1835
BstNI CCWGG 1 cut(s) 633
BstNSI RCATGY 1 cut(s) 1229
BstSCI CCNGG 5 cut(s) 457, 458, 631, 1372, 1547
BstSFI CTRYAG 3 cut(s) 661, 1321, 1673
BstSLI GKGCMC 2 cut(s) 1642, 1965
BstV1I GCAGC 6 cut(s) 139, 284, 648, 1368, 1738, 1822
BstV2I GAAGAC 2 cut(s) 1319, 1385
BstX2I RGATCY 1 cut(s) 1057
BstYI RGATCY 1 cut(s) 1057
BsuI GTATCC 1 cut(s) 1210
BsuRI GGCC 5 cut(s) 473, 980, 988, 1206, 1647
BtgI CCRYGG 2 cut(s) 1118, 1957
BtgZI GCGATG 2 cut(s) 1578, 1734
BtrI CACGTC 2 cut(s) 379, 1505
BtsCI GGATG 4 cut(s) 628, 787, 961, 1299
BtsI GCAGTG 1 cut(s) 1670
BtsIMutI CAGTG 4 cut(s) 670, 1272, 1640, 1670
BveI ACCTGC 1 cut(s) 774
Cac8I GCNNGC 6 cut(s) 168, 443, 564, 1649, 1677, 1876
CaiI CAGNNNCTG 1 cut(s) 133
CfoI GCGC 2 cut(s) 1187, 1430
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 2 cut(s) 803, 1645
Cfr9I CCCGGG 1 cut(s) 458
CseI GACGC 1 cut(s) 963
Csp6I GTAC 5 cut(s) 211, 832, 1466, 1768, 1811
CviAII CATG 5 cut(s) 507, 1226, 1291, 1314, 1919
CviQI GTAC 5 cut(s) 211, 832, 1466, 1768, 1811
DdeI CTNAG 9 cut(s) 313, 555, 990, 1061, 1137, 1424, 1442, 1475, 1886
DraI TTTAAA 1 cut(s) 1909
DrdI GACNNNNNNGTC 1 cut(s) 1147
DseDI GACNNNNNNGTC 1 cut(s) 1147
Eam1104I CTCTTC 4 cut(s) 220, 841, 1206, 1382
EarI CTCTTC 4 cut(s) 220, 841, 1206, 1382
Ecl136II GAGCTC 2 cut(s) 423, 1537
Eco130I CCWWGG 1 cut(s) 318
Eco147I AGGCCT 1 cut(s) 1206
Eco24I GRGCYC 4 cut(s) 245, 311, 425, 1539
Eco47I GGWCC 1 cut(s) 803
Eco53kI GAGCTC 2 cut(s) 423, 1537
Eco57I CTGAAG 3 cut(s) 822, 1180, 1610
Eco88I CYCGRG 2 cut(s) 426, 458
EcoICRI GAGCTC 2 cut(s) 423, 1537
EcoNI CCTNNNNNAGG 1 cut(s) 1211
EcoRII CCWGG 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 318
EcoT38I GRGCYC 4 cut(s) 245, 311, 425, 1539
ErhI CCWWGG 1 cut(s) 318
Esp3I CGTCTC 1 cut(s) 1117
FaeI CATG 5 cut(s) 510, 1229, 1294, 1317, 1922
FalI AAGNNNNNCTT 6 cut(s) 102, 134, 1174, 1206, 1824, 1856
FaqI GGGAC 1 cut(s) 789
FatI CATG 5 cut(s) 506, 1225, 1290, 1313, 1918
FauI CCCGC 1 cut(s) 434
FbaI TGATCA 1 cut(s) 337
Fnu4HI GCNGC 7 cut(s) 128, 273, 276, 637, 1382, 1727, 1836
FokI GGATG 4 cut(s) 635, 794, 968, 1286
FriOI GRGCYC 4 cut(s) 245, 311, 425, 1539
Fsp4HI GCNGC 7 cut(s) 128, 273, 276, 637, 1382, 1727, 1836
FspBI CTAG 7 cut(s) 225, 642, 860, 1173, 1494, 1763, 1863
GlaI GCGC 2 cut(s) 1186, 1429
GluI GCNGC 7 cut(s) 128, 273, 276, 637, 1382, 1727, 1836
GsaI CCCAGC 1 cut(s) 955
GsuI CTGGAG 1 cut(s) 924
HaeII RGCGCY 2 cut(s) 1188, 1431
HaeIII GGCC 5 cut(s) 473, 980, 988, 1206, 1647
HapII CCGG 4 cut(s) 167, 459, 1373, 1548
HgaI GACGC 1 cut(s) 963
HhaI GCGC 2 cut(s) 1187, 1430
Hin1II CATG 5 cut(s) 510, 1229, 1294, 1317, 1922
Hin6I GCGC 2 cut(s) 1185, 1428
HinP1I GCGC 2 cut(s) 1185, 1428
HincII GTYRAC 1 cut(s) 1339
HindII GTYRAC 1 cut(s) 1339
HindIII AAGCTT 2 cut(s) 494, 1883
HpaI GTTAAC 1 cut(s) 1339
HpaII CCGG 4 cut(s) 167, 459, 1373, 1548
HphI GGTGA 6 cut(s) 92, 386, 480, 879, 1063, 1807
Hpy166II GTNNAC 3 cut(s) 88, 1339, 1768
Hpy188I TCNGA 8 cut(s) 135, 179, 346, 409, 841, 993, 1064, 1502
Hpy188III TCNNGA 9 cut(s) 426, 584, 717, 1012, 1035, 1136, 1494, 1604, 1705
Hpy8I GTNNAC 3 cut(s) 88, 1339, 1768
Hpy99I CGWCG 1 cut(s) 979
HpyAV CCTTC 6 cut(s) 40, 278, 613, 723, 1430, 1525
HpyCH4III ACNGT 6 cut(s) 141, 665, 1237, 1267, 1600, 1741
HpyCH4IV ACGT 2 cut(s) 378, 1504
HpyF10VI GCNNNNNNNGC 5 cut(s) 115, 124, 688, 1591, 1835
HpyF3I CTNAG 9 cut(s) 313, 555, 990, 1061, 1137, 1424, 1442, 1475, 1886
HpySE526I ACGT 2 cut(s) 378, 1504
Hsp92II CATG 5 cut(s) 510, 1229, 1294, 1317, 1922
HspAI GCGC 2 cut(s) 1185, 1428
KroI GCCGGC 1 cut(s) 166
KroNI GCCGGC 1 cut(s) 168
Ksp22I TGATCA 1 cut(s) 337
KspAI GTTAAC 1 cut(s) 1339
LmnI GCTCC 4 cut(s) 240, 332, 753, 1534
Lsp1109I GCAGC 6 cut(s) 139, 284, 648, 1368, 1738, 1822
LweI GCATC 5 cut(s) 494, 549, 669, 772, 1910
MaeI CTAG 7 cut(s) 225, 642, 860, 1173, 1494, 1763, 1863
MaeII ACGT 2 cut(s) 378, 1504
MaeIII GTNAC 4 cut(s) 374, 379, 1156, 1478
MflI RGATCY 1 cut(s) 1057
MhlI GDGCHC 7 cut(s) 245, 300, 311, 425, 1539, 1642, 1965
MluCI AATT 8 cut(s) 8, 27, 96, 367, 514, 728, 908, 1252
MlyI GAGTC 6 cut(s) 189, 376, 517, 1135, 1153, 1399
MmeI TCCRAC 3 cut(s) 123, 378, 894
MroNI GCCGGC 1 cut(s) 166
MroXI GAANNNNTTC 2 cut(s) 743, 1352
MseI TTAA 6 cut(s) 26, 51, 540, 1338, 1451, 1908
MslI CAYNNNNRTG 3 cut(s) 450, 663, 969
MspA1I CMGCKG 2 cut(s) 275, 1651
MspI CCGG 4 cut(s) 167, 459, 1373, 1548
MspR9I CCNGG 5 cut(s) 459, 460, 633, 1374, 1549
Mva1269I GAATGC 1 cut(s) 691
MvaI CCWGG 1 cut(s) 633
MwoI GCNNNNNNNGC 5 cut(s) 115, 124, 688, 1591, 1835
NaeI GCCGGC 1 cut(s) 168
NciI CCSGG 4 cut(s) 459, 460, 1374, 1549
NgoMIV GCCGGC 1 cut(s) 166
NlaIII CATG 5 cut(s) 510, 1229, 1294, 1317, 1922
NlaIV GGNNCC 5 cut(s) 242, 755, 764, 805, 1859
NmeAIII GCCGAG 2 cut(s) 499, 1226
NmuCI GTSAC 3 cut(s) 374, 379, 1156
NspI RCATGY 1 cut(s) 1229
NspV TTCGAA 1 cut(s) 569
OliI CACNNNNGTG 2 cut(s) 663, 969
PaeR7I CTCGAG 1 cut(s) 426
PceI AGGCCT 1 cut(s) 1206
PctI GAATGC 1 cut(s) 691
PdiI GCCGGC 1 cut(s) 168
PdmI GAANNNNTTC 2 cut(s) 743, 1352
PfeI GAWTC 5 cut(s) 65, 416, 884, 1107, 1497
PflMI CCANNNNNTGG 1 cut(s) 1654
PfoI TCCNGGA 1 cut(s) 1547
PkrI GCNGC 7 cut(s) 129, 274, 277, 638, 1383, 1728, 1837
PleI GAGTC 6 cut(s) 188, 376, 517, 1135, 1153, 1398
PpsI GAGTC 6 cut(s) 188, 376, 517, 1135, 1153, 1398
Psp124BI GAGCTC 2 cut(s) 425, 1539
Psp6I CCWGG 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 951
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 5 cut(s) 242, 755, 764, 805, 1859
PspPI GGNCC 2 cut(s) 803, 1645
PstI CTGCAG 2 cut(s) 1325, 1677
PstNI CAGNNNCTG 1 cut(s) 133
PsuI RGATCY 1 cut(s) 1057
PvuII CAGCTG 1 cut(s) 1651
RsaI GTAC 5 cut(s) 212, 833, 1467, 1769, 1812
RsaNI GTAC 5 cut(s) 211, 832, 1466, 1768, 1811
RseI CAYNNNNRTG 3 cut(s) 450, 663, 969
SacI GAGCTC 2 cut(s) 425, 1539
SaqAI TTAA 6 cut(s) 26, 51, 540, 1338, 1451, 1908
SatI GCNGC 7 cut(s) 128, 273, 276, 637, 1382, 1727, 1836
Sau96I GGNCC 2 cut(s) 803, 1645
ScaI AGTACT 2 cut(s) 212, 1467
SchI GAGTC 6 cut(s) 189, 376, 517, 1135, 1153, 1399
ScrFI CCNGG 5 cut(s) 459, 460, 633, 1374, 1549
SduI GDGCHC 7 cut(s) 245, 300, 311, 425, 1539, 1642, 1965
SfaNI GCATC 5 cut(s) 494, 549, 669, 772, 1910
SfcI CTRYAG 3 cut(s) 661, 1321, 1673
Sfr274I CTCGAG 1 cut(s) 426
SfuI TTCGAA 1 cut(s) 569
SinI GGWCC 1 cut(s) 803
SlaI CTCGAG 1 cut(s) 426
SmaI CCCGGG 1 cut(s) 460
SmiMI CAYNNNNRTG 3 cut(s) 450, 663, 969
SmlI CTYRAG 2 cut(s) 426, 1609
SmoI CTYRAG 2 cut(s) 426, 1609
Sse9I AATT 8 cut(s) 8, 27, 96, 367, 514, 728, 908, 1252
SseBI AGGCCT 1 cut(s) 1206
SsiI CCGC 2 cut(s) 275, 441
SspMI CTAG 7 cut(s) 225, 642, 860, 1173, 1494, 1763, 1863
SstI GAGCTC 2 cut(s) 425, 1539
StuI AGGCCT 1 cut(s) 1206
StyD4I CCNGG 5 cut(s) 457, 458, 631, 1372, 1547
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 6 cut(s) 141, 665, 1237, 1267, 1600, 1741
TaiI ACGT 2 cut(s) 381, 1507
TasI AATT 8 cut(s) 8, 27, 96, 367, 514, 728, 908, 1252
TatI WGTACW 2 cut(s) 210, 1465
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 5 cut(s) 65, 416, 884, 1107, 1497
Tru1I TTAA 6 cut(s) 26, 51, 540, 1338, 1451, 1908
Tru9I TTAA 6 cut(s) 26, 51, 540, 1338, 1451, 1908
TscAI CASTG 4 cut(s) 670, 1272, 1647, 1677
TseFI GTSAC 3 cut(s) 374, 379, 1156
TseI GCWGC 6 cut(s) 127, 272, 636, 1381, 1726, 1835
Tsp45I GTSAC 3 cut(s) 374, 379, 1156
TspDTI ATGAA 7 cut(s) 78, 375, 954, 1104, 1302, 1307, 1345
TspGWI ACGGA 3 cut(s) 896, 1066, 1135
TspMI CCCGGG 1 cut(s) 458
TspRI CASTG 4 cut(s) 670, 1272, 1647, 1677
Van91I CCANNNNNTGG 1 cut(s) 1654
VpaK11BI GGWCC 1 cut(s) 803
XagI CCTNNNNNAGG 1 cut(s) 1211
XapI RAATTY 2 cut(s) 8, 1252
XbaI TCTAGA 1 cut(s) 1493
XceI RCATGY 1 cut(s) 1229
XhoI CTCGAG 1 cut(s) 426
XmaI CCCGGG 1 cut(s) 458
XmnI GAANNNNTTC 2 cut(s) 743, 1352
XspI CTAG 7 cut(s) 225, 642, 860, 1173, 1494, 1763, 1863
ZrmI AGTACT 2 cut(s) 212, 1467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.