Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002669

Physical Location & Seq

Genomic Coordinates Forward (+)

23633956 .. 23638027

Gene Structure 4072 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1187900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1572 bp

ATGGGTTTGGGCGGGACAACCTTGCAAACCCTGAAGCTCGCCACTATTCCTCCACCCTCATTTTCTTCTTCTTTCCGCTCCGTTTTCAAACCCTTTTCCCAAACCTTCCTCTGCCACCGTAGCCGCAGACAACGATTTCCATTTCCTCTGCCCTTCTTCGTCAGAGGCCTTTCGGCTCCGGCCGCACAGGCAACTGCTACCGCAACCCCAAAAGAAACAAGCCCGGATGAAAAGGGTGTGAAGCAGCAATGGAAAGCAGCGATAGACTTCAAGTGGATAAGAGACAACAAGGACGCGGTTGCGGCCAACATAAAGAAGAGGAACTCCAATGCCAATTTGGAACTTGTGCTTGAGCTCTACCACAAAATGCTGAGTCTTCAAAAGGAAGTTGAACGGCTTCGTGGGGAAAGGAATGTGGTGGCAAACAAGATGAAAGGAAAGTTGGAGCCATCCGAGCGTCAAAAACTTATAGATGAAGGAAAGAATCTGAAGGAAGGGCTTCTTTCTTTGGAAGAAGACCTGCTTAGACTTACTGATGAGCTTCAGCAGGAAGCACAATGTATACCAAATATGACTCATCCTGATGTCCCAATAGGCGGAGAGGATTTTTCAACCTTAAGAAATATGGTAGGCAGCCCACGTGAGTTTAGCTTTCCTGTCAAGGATCACCTTCAACTTGGAAAGGAACTAGATCTTTTTGATTTTGATGCTGCTTCAGGGGTCAGTGGCTCAAAGTTTTACTATCTGAAGAATGAAGCGGTACTCCTAGAGATGGCCCTTATTAACTGGACGCTCTTGGAAGTCATGAAAAGGGGCTTCACACCTTTAACAACCCCTGAAATTGTAAGATCTTCTGTTGTTGAAAAATGTGGTTTCCAACCCCGAGGAGCAAATACCCAGGTGTATTCTATTGAGGATAGTGATCAGTGCCTCATCGGCACAGCAGAGATTCCTGTTGGGGGAATTCATATGGATTCTATACTTGCTGCATCATTGTTACCTCTGAAATATGTGGCAGTTTCCCATTGCTTCCGTACTGAGGCAGGCGCTGCAGGTACAGCATCAAGGGGTCTGTATCGCGTCCACCAATTCAGCAAGCTGGAGATGTTTATATTATGCCGACCCGAGGATAGTGATTTGTACCATGAAGAGCTCATAAGAATTGAAGAAGACCTGTTTTCATCGCTAGGATTACACTACAAAACTTTGGACATGGCATCTGGGGATTTAGGTGCGCCTGCTTATCGTAAATTTGATATTGAAGCATGGATGCCTGGTTTAGCAAGATATGGAGAGATATCAAGCGCATCGAACTGTACAGACTATCAAAGTCGTCGTCTTGGGATCCGGTTTCGTTCTGCAGAACCAGCGTTAACAAATCCTAAGAAGGGCAAAAGCAGCCTCGCTCCACCAGAGTTTGTTCACACACTGAATGCAACGGCCTGTGCAGTACCACGAATGATCGTATGCATACTTGAGAACAACCAGCAAGAAGATGGCTCGGTCATTATCCCTGAGCCATTGAGGCCCTTTATGGGCGGACTTGAGTCCATATATCCCAAACCTAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004828 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006434 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006996 GO:0007005 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0035670 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0071704 GO:0071840 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Seryl_tRNA_N tRNA-synt_2b

Protein Analysis

524

Amino Acids

58.33

Weight (kDa)

7.13

Isoelectric Point (pI)

54.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Seryl_tRNA_N	PF02403	88 - 191	3.8e-20	Seryl-tRNA synthetase N-terminal domain
tRNA-synt_2b	PF00587	306 - 495	2.5e-30	tRNA synthetase class II core domain (G, H, P, S and T)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002669)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11870 AT1G11870 AT1G11870 AT1G11870 AT1G11870 AT1G11870
fragaria_vesca	FvH4_5g36670 FvH4_5g36670 FvH4_5g36670 FvH4_5g36670 FvH4_5g36670 FvH4_5g36670 FvH4_5g36670 FvH4_5g36670 FvH4_5g36670 FvH4_5g36670 FvH4_5g36670 FvH4_5g36670 FvH4_5g36670
malus_domestica	MD08G1187900.v1.1 MD15G1373800.v1.1
prunus_persica	Prupe.1G522400_v2.0.a1 Prupe.1G522400_v2.0.a1
pyrus_communis	pycom08g16120 pycom15g33480 pycom15g33490
rosa_chinensis	RchiOBHm_Chr7g0242571
rosa_laevigata	RLG00000000891
rosa_multiflora	Rmu_sc0000429.1_g000097
rosa_roxburghii	Rroxscaffold_3G00222620
rosa_rugosa	Rorug07G0315500 Rorug07G0315600 Rorug07G0315700 Rorug07G0315800
rosa_samantha	Rh7AG470900 Rh7BG441200 Rh7CG487800 Rh7DG456400
rosa_wichuraiana	Rw0G005480 Rw7G039270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	528, 1043
AccBSI	CCGCTC	1 cut(s)	78
AccI	GTMKAC	1 cut(s)	562
AccII	CGCG	2 cut(s)	296, 1080
AclWI	GGATC	3 cut(s)	672, 1339, 1352
AcoI	YGGCCR	2 cut(s)	180, 303
AcsI	RAATTY	2 cut(s)	963, 1250
AcuI	CTGAAG	5 cut(s)	53, 509, 527, 699, 767
AcvI	CACGTG	1 cut(s)	641
AfaI	GTAC	6 cut(s)	762, 1036, 1057, 1142, 1318, 1452
AfiI	CCNNNNNNNGG	8 cut(s)	596, 772, 959, 1039, 1126, 1388, 1535, 1566
AflII	CTTAAG	1 cut(s)	616
AgsI	TTSAA	9 cut(s)	88, 271, 380, 392, 612, 674, 863, 1166, 1262
AjnI	CCWGG	2 cut(s)	897, 1273
AjuI	GAANNNNNNNTTGG	2 cut(s)	425, 457
AluBI	AGCT	6 cut(s)	37, 355, 541, 651, 1099, 1153
AluI	AGCT	6 cut(s)	37, 355, 541, 651, 1099, 1153
Alw21I	GWGCWC	2 cut(s)	357, 1155
Alw26I	GTCTC	1 cut(s)	276
AlwI	GGATC	3 cut(s)	672, 1339, 1352
AlwNI	CAGNNNCTG	1 cut(s)	1049
Ama87I	CYCGRG	2 cut(s)	882, 1124
AoxI	GGCC	6 cut(s)	166, 180, 303, 774, 1440, 1526
ApeKI	GCWGC	7 cut(s)	244, 257, 633, 710, 986, 1049, 1398
ApoI	RAATTY	2 cut(s)	963, 1250
ArsI	GACNNNNNNTTYG	2 cut(s)	1321, 1353
Asp700I	GAANNNNTTC	2 cut(s)	396, 498
AspA2I	CCTAGG	1 cut(s)	1565
AspLEI	GCGC	3 cut(s)	1049, 1237, 1307
AspS9I	GGNCC	2 cut(s)	775, 1527
AsuC2I	CCSGG	1 cut(s)	224
AsuHPI	GGTGA	1 cut(s)	659
AvaI	CYCGRG	2 cut(s)	882, 1124
AvrII	CCTAGG	1 cut(s)	1565
BamHI	GGATCC	1 cut(s)	1344
BanII	GRGCYC	2 cut(s)	357, 1155
BbrPI	CACGTG	1 cut(s)	641
BbsI	GAAGAC	3 cut(s)	368, 522, 1176
Bbv12I	GWGCWC	2 cut(s)	357, 1155
BbvI	GCAGC	7 cut(s)	256, 269, 645, 697, 973, 1036, 1410
BccI	CCATC	3 cut(s)	457, 766, 1490
BceAI	ACGGC	2 cut(s)	410, 1455
BciT130I	CCWGG	2 cut(s)	899, 1275
BclI	TGATCA	1 cut(s)	922
BcnI	CCSGG	1 cut(s)	224
BcoDI	GTCTC	1 cut(s)	276
BfaI	CTAG	4 cut(s)	689, 767, 1187, 1566
BfmI	CTRYAG	2 cut(s)	1050, 1359
BfoI	RGCGCY	1 cut(s)	1050
BfrI	CTTAAG	1 cut(s)	616
BfuAI	ACCTGC	2 cut(s)	528, 1043
BglI	GCCNNNNNGGC	3 cut(s)	188, 936, 1525
BglII	AGATCT	2 cut(s)	691, 848
BlnI	CCTAGG	1 cut(s)	1565
Bme1390I	CCNGG	3 cut(s)	224, 899, 1275
BmeT110I	CYCGRG	2 cut(s)	882, 1124
BmgT120I	GGNCC	2 cut(s)	775, 1527
BmiI	GGNNCC	3 cut(s)	177, 447, 1346
BmrFI	CCNGG	3 cut(s)	224, 899, 1275
BmsI	GCATC	6 cut(s)	697, 998, 1070, 1226, 1260, 1316
BoxI	GACNNNNGTC	1 cut(s)	1546
BpiI	GAAGAC	3 cut(s)	368, 522, 1176
BpmI	CTGGAG	1 cut(s)	1121
Bpu10I	CCTNAGC	1 cut(s)	1515
BpuEI	CTTGAG	3 cut(s)	371, 1496, 1565
BpuMI	CCSGG	1 cut(s)	224
BsaAI	YACGTR	1 cut(s)	641
BsaBI	GATNNNNATC	1 cut(s)	921
BsaJI	CCNNGG	4 cut(s)	883, 897, 1125, 1565
BsaWI	WCCGGW	1 cut(s)	1347
BsaXI	ACNNNNNCTCC	2 cut(s)	34, 64
Bsc4I	CCNNNNNNNGG	8 cut(s)	596, 772, 959, 1039, 1126, 1388, 1535, 1566
Bse1I	ACTGG	1 cut(s)	791
Bse3DI	GCAATG	2 cut(s)	254, 1024
Bse8I	GATNNNNATC	1 cut(s)	921
BseBI	CCWGG	2 cut(s)	899, 1275
BseDI	CCNNGG	4 cut(s)	883, 897, 1125, 1565
BseGI	GGATG	4 cut(s)	232, 449, 577, 1275
BseJI	GATNNNNATC	1 cut(s)	921
BseLI	CCNNNNNNNGG	8 cut(s)	596, 772, 959, 1039, 1126, 1388, 1535, 1566
BseMI	GCAATG	2 cut(s)	254, 1024
BseMII	CTCAG	3 cut(s)	362, 1029, 1506
BseNI	ACTGG	1 cut(s)	791
BseRI	GAGGAG	1 cut(s)	900
BseX3I	CGGCCG	1 cut(s)	180
BseXI	GCAGC	7 cut(s)	256, 269, 645, 697, 973, 1036, 1410
BsgI	GTGCAG	1 cut(s)	1467
Bsh1236I	CGCG	2 cut(s)	296, 1080
Bsh1285I	CGRYCG	1 cut(s)	183
BshFI	GGCC	6 cut(s)	168, 182, 305, 776, 1442, 1528
BsiEI	CGRYCG	1 cut(s)	183
BsiHKAI	GWGCWC	2 cut(s)	357, 1155
BsiHKCI	CYCGRG	2 cut(s)	882, 1124
BsiSI	CCGG	3 cut(s)	179, 224, 1348
BslFI	GGGAC	2 cut(s)	28, 572
BslI	CCNNNNNNNGG	8 cut(s)	596, 772, 959, 1039, 1126, 1388, 1535, 1566
BsmAI	GTCTC	1 cut(s)	276
BsmFI	GGGAC	2 cut(s)	28, 572
BsmI	GAATGC	1 cut(s)	1438
BsnI	GGCC	6 cut(s)	168, 182, 305, 776, 1442, 1528
BsoBI	CYCGRG	2 cut(s)	882, 1124
Bsp1286I	GDGCHC	2 cut(s)	357, 1155
Bsp1407I	TGTACA	1 cut(s)	1316
Bsp143I	GATC	6 cut(s)	664, 691, 848, 922, 1344, 1461
BspANI	GGCC	6 cut(s)	168, 182, 305, 776, 1442, 1528
BspCNI	CTCAG	3 cut(s)	363, 1030, 1507
BspFNI	CGCG	2 cut(s)	296, 1080
BspHI	TCATGA	1 cut(s)	804
BspLI	GGNNCC	3 cut(s)	177, 447, 1346
BspMAI	CTGCAG	2 cut(s)	1054, 1363
BspMI	ACCTGC	2 cut(s)	528, 1043
BspPI	GGATC	3 cut(s)	672, 1339, 1352
BspQI	GCTCTTC	1 cut(s)	1143
BspTI	CTTAAG	1 cut(s)	616
BsrBI	CCGCTC	1 cut(s)	78
BsrDI	GCAATG	2 cut(s)	254, 1024
BsrGI	TGTACA	1 cut(s)	1316
BsrI	ACTGG	1 cut(s)	791
BssECI	CCNNGG	4 cut(s)	883, 897, 1125, 1565
BssMI	GATC	6 cut(s)	664, 691, 848, 922, 1344, 1461
BssNAI	GTATAC	1 cut(s)	563
BssT1I	CCWWGG	1 cut(s)	1565
Bst1107I	GTATAC	1 cut(s)	563
Bst2UI	CCWGG	2 cut(s)	899, 1275
Bst4CI	ACNGT	2 cut(s)	119, 1316
Bst6I	CTCTTC	2 cut(s)	311, 1143
BstAFI	CTTAAG	1 cut(s)	616
BstAPI	GCANNNNNTGC	1 cut(s)	1049
BstAUI	TGTACA	1 cut(s)	1316
BstBAI	YACGTR	1 cut(s)	641
BstC8I	GCNNGC	4 cut(s)	39, 1045, 1097, 1239
BstDEI	CTNAG	5 cut(s)	371, 524, 1038, 1383, 1515
BstF5I	GGATG	4 cut(s)	232, 449, 577, 1275
BstFNI	CGCG	2 cut(s)	296, 1080
BstH2I	RGCGCY	1 cut(s)	1050
BstHHI	GCGC	3 cut(s)	1049, 1237, 1307
BstKTI	GATC	6 cut(s)	667, 694, 851, 925, 1347, 1464
BstMAI	GTCTC	1 cut(s)	276
BstMBI	GATC	6 cut(s)	664, 691, 848, 922, 1344, 1461
BstMCI	CGRYCG	1 cut(s)	183
BstNI	CCWGG	2 cut(s)	899, 1275
BstPAI	GACNNNNGTC	1 cut(s)	1546
BstSCI	CCNGG	3 cut(s)	222, 897, 1273
BstSFI	CTRYAG	2 cut(s)	1050, 1359
BstUI	CGCG	2 cut(s)	296, 1080
BstV1I	GCAGC	7 cut(s)	256, 269, 645, 697, 973, 1036, 1410
BstV2I	GAAGAC	3 cut(s)	368, 522, 1176
BstX2I	RGATCY	3 cut(s)	691, 848, 1344
BstYI	RGATCY	3 cut(s)	691, 848, 1344
BstZ17I	GTATAC	1 cut(s)	563
BstZI	CGGCCG	1 cut(s)	180
BsuRI	GGCC	6 cut(s)	168, 182, 305, 776, 1442, 1528
BtgZI	GCGATG	1 cut(s)	1167
BtsCI	GGATG	4 cut(s)	232, 449, 577, 1275
BtsIMutI	CAGTG	3 cut(s)	730, 932, 1427
BveI	ACCTGC	2 cut(s)	528, 1043
Cac8I	GCNNGC	4 cut(s)	39, 1045, 1097, 1239
CaiI	CAGNNNCTG	1 cut(s)	1049
CciI	TCATGA	1 cut(s)	804
CfoI	GCGC	3 cut(s)	1049, 1237, 1307
Cfr13I	GGNCC	2 cut(s)	775, 1527
CseI	GACGC	4 cut(s)	302, 446, 799, 1069
Csp6I	GTAC	6 cut(s)	761, 1035, 1056, 1141, 1317, 1451
CviAII	CATG	4 cut(s)	805, 1145, 1213, 1266
CviQI	GTAC	6 cut(s)	761, 1035, 1056, 1141, 1317, 1451
DdeI	CTNAG	5 cut(s)	371, 524, 1038, 1383, 1515
DpnI	GATC	6 cut(s)	666, 693, 850, 924, 1346, 1463
DpnII	GATC	6 cut(s)	664, 691, 848, 922, 1344, 1461
EaeI	YGGCCR	2 cut(s)	180, 303
EagI	CGGCCG	1 cut(s)	180
Eam1104I	CTCTTC	2 cut(s)	311, 1143
EarI	CTCTTC	2 cut(s)	311, 1143
EciI	GGCGGA	2 cut(s)	612, 1554
Ecl136II	GAGCTC	2 cut(s)	355, 1153
EclXI	CGGCCG	1 cut(s)	180
Eco130I	CCWWGG	1 cut(s)	1565
Eco147I	AGGCCT	1 cut(s)	168
Eco24I	GRGCYC	2 cut(s)	357, 1155
Eco32I	GATATC	1 cut(s)	1299
Eco52I	CGGCCG	1 cut(s)	180
Eco53kI	GAGCTC	2 cut(s)	355, 1153
Eco57I	CTGAAG	5 cut(s)	53, 509, 527, 699, 767
Eco72I	CACGTG	1 cut(s)	641
Eco88I	CYCGRG	2 cut(s)	882, 1124
EcoICRI	GAGCTC	2 cut(s)	355, 1153
EcoO109I	RGGNCCY	1 cut(s)	1527
EcoRI	GAATTC	1 cut(s)	963
EcoRII	CCWGG	2 cut(s)	897, 1273
EcoRV	GATATC	1 cut(s)	1299
EcoT14I	CCWWGG	1 cut(s)	1565
EcoT22I	ATGCAT	1 cut(s)	1472
EcoT38I	GRGCYC	2 cut(s)	357, 1155
ErhI	CCWWGG	1 cut(s)	1565
FaeI	CATG	4 cut(s)	808, 1148, 1216, 1269
FalI	AAGNNNNNCTT	4 cut(s)	486, 518, 507, 539
FaqI	GGGAC	2 cut(s)	28, 572
FatI	CATG	4 cut(s)	804, 1144, 1212, 1265
FauI	CCCGC	1 cut(s)	5
FauNDI	CATATG	1 cut(s)	969
FbaI	TGATCA	1 cut(s)	922
FblI	GTMKAC	1 cut(s)	562
FokI	GGATG	4 cut(s)	239, 436, 564, 1282
FriOI	GRGCYC	2 cut(s)	357, 1155
FspBI	CTAG	4 cut(s)	689, 767, 1187, 1566
GlaI	GCGC	3 cut(s)	1048, 1236, 1306
GsuI	CTGGAG	1 cut(s)	1121
HaeII	RGCGCY	1 cut(s)	1050
HaeIII	GGCC	6 cut(s)	168, 182, 305, 776, 1442, 1528
HapII	CCGG	3 cut(s)	179, 224, 1348
HgaI	GACGC	4 cut(s)	302, 446, 799, 1069
HhaI	GCGC	3 cut(s)	1049, 1237, 1307
Hin1II	CATG	4 cut(s)	808, 1148, 1216, 1269
Hin6I	GCGC	3 cut(s)	1047, 1235, 1305
HinP1I	GCGC	3 cut(s)	1047, 1235, 1305
HincII	GTYRAC	1 cut(s)	1374
HindII	GTYRAC	1 cut(s)	1374
HinfI	GANTC	6 cut(s)	373, 484, 574, 949, 974, 1547
HpaI	GTTAAC	1 cut(s)	1374
HpaII	CCGG	3 cut(s)	179, 224, 1348
HphI	GGTGA	1 cut(s)	659
Hpy166II	GTNNAC	4 cut(s)	563, 1084, 1374, 1423
Hpy188I	TCNGA	5 cut(s)	164, 454, 489, 747, 1005
Hpy188III	TCNNGA	2 cut(s)	581, 805
Hpy8I	GTNNAC	4 cut(s)	563, 1084, 1374, 1423
Hpy99I	CGWCG	1 cut(s)	1338
HpyAV	CCTTC	7 cut(s)	115, 163, 470, 484, 488, 680, 1381
HpyCH4III	ACNGT	2 cut(s)	119, 1316
HpyCH4IV	ACGT	1 cut(s)	640
HpyCH4V	TGCA	7 cut(s)	25, 989, 1052, 1361, 1436, 1448, 1470
HpyF3I	CTNAG	5 cut(s)	371, 524, 1038, 1383, 1515
HpySE526I	ACGT	1 cut(s)	640
Hsp92II	CATG	4 cut(s)	808, 1148, 1216, 1269
HspAI	GCGC	3 cut(s)	1047, 1235, 1305
Ksp22I	TGATCA	1 cut(s)	922
KspAI	GTTAAC	1 cut(s)	1374
Kzo9I	GATC	6 cut(s)	664, 691, 848, 922, 1344, 1461
LguI	GCTCTTC	1 cut(s)	1143
LmnI	GCTCC	5 cut(s)	83, 181, 445, 887, 1411
Lsp1109I	GCAGC	7 cut(s)	256, 269, 645, 697, 973, 1036, 1410
LweI	GCATC	6 cut(s)	697, 998, 1070, 1226, 1260, 1316
MaeI	CTAG	4 cut(s)	689, 767, 1187, 1566
MaeII	ACGT	1 cut(s)	640
MaeIII	GTNAC	1 cut(s)	996
MalI	GATC	6 cut(s)	666, 693, 850, 924, 1346, 1463
MbiI	CCGCTC	1 cut(s)	78
MboI	GATC	6 cut(s)	664, 691, 848, 922, 1344, 1461
MflI	RGATCY	3 cut(s)	691, 848, 1344
MhlI	GDGCHC	2 cut(s)	357, 1155
MluCI	AATT	6 cut(s)	334, 840, 963, 1088, 1161, 1250
MlyI	GAGTC	3 cut(s)	382, 568, 1556
MmeI	TCCRAC	2 cut(s)	423, 901
Mph1103I	ATGCAT	1 cut(s)	1472
MroXI	GAANNNNTTC	2 cut(s)	396, 498
MseI	TTAA	4 cut(s)	617, 783, 827, 1373
MslI	CAYNNNNRTG	1 cut(s)	582
MspCI	CTTAAG	1 cut(s)	616
MspI	CCGG	3 cut(s)	179, 224, 1348
MspR9I	CCNGG	3 cut(s)	224, 899, 1275
Mva1269I	GAATGC	1 cut(s)	1438
MvaI	CCWGG	2 cut(s)	899, 1275
MvnI	CGCG	2 cut(s)	296, 1080
NciI	CCSGG	1 cut(s)	224
NdeI	CATATG	1 cut(s)	969
NdeII	GATC	6 cut(s)	664, 691, 848, 922, 1344, 1461
NlaIII	CATG	4 cut(s)	808, 1148, 1216, 1269
NlaIV	GGNNCC	3 cut(s)	177, 447, 1346
NsiI	ATGCAT	1 cut(s)	1472
PagI	TCATGA	1 cut(s)	804
PceI	AGGCCT	1 cut(s)	168
PciSI	GCTCTTC	1 cut(s)	1143
PctI	GAATGC	1 cut(s)	1438
PdmI	GAANNNNTTC	2 cut(s)	396, 498
PfeI	GAWTC	3 cut(s)	484, 949, 974
PleI	GAGTC	3 cut(s)	381, 568, 1555
PmaCI	CACGTG	1 cut(s)	641
PmlI	CACGTG	1 cut(s)	641
PpsI	GAGTC	3 cut(s)	381, 568, 1555
Ppu21I	YACGTR	1 cut(s)	641
PshAI	GACNNNNGTC	1 cut(s)	1546
Psp124BI	GAGCTC	2 cut(s)	357, 1155
Psp6I	CCWGG	2 cut(s)	897, 1273
PspCI	CACGTG	1 cut(s)	641
PspGI	CCWGG	2 cut(s)	897, 1273
PspN4I	GGNNCC	3 cut(s)	177, 447, 1346
PspPI	GGNCC	2 cut(s)	775, 1527
PstI	CTGCAG	2 cut(s)	1054, 1363
PstNI	CAGNNNCTG	1 cut(s)	1049
PsuI	RGATCY	3 cut(s)	691, 848, 1344
RsaI	GTAC	6 cut(s)	762, 1036, 1057, 1142, 1318, 1452
RsaNI	GTAC	6 cut(s)	761, 1035, 1056, 1141, 1317, 1451
RseI	CAYNNNNRTG	1 cut(s)	582
SacI	GAGCTC	2 cut(s)	357, 1155
SapI	GCTCTTC	1 cut(s)	1143
SaqAI	TTAA	4 cut(s)	617, 783, 827, 1373
Sau3AI	GATC	6 cut(s)	664, 691, 848, 922, 1344, 1461
Sau96I	GGNCC	2 cut(s)	775, 1527
SchI	GAGTC	3 cut(s)	382, 568, 1556
ScrFI	CCNGG	3 cut(s)	224, 899, 1275
SduI	GDGCHC	2 cut(s)	357, 1155
SfaNI	GCATC	6 cut(s)	697, 998, 1070, 1226, 1260, 1316
SfcI	CTRYAG	2 cut(s)	1050, 1359
SmiMI	CAYNNNNRTG	1 cut(s)	582
SmlI	CTYRAG	4 cut(s)	350, 616, 1475, 1544
SmoI	CTYRAG	4 cut(s)	350, 616, 1475, 1544
Sse9I	AATT	6 cut(s)	334, 840, 963, 1088, 1161, 1250
SseBI	AGGCCT	1 cut(s)	168
SspMI	CTAG	4 cut(s)	689, 767, 1187, 1566
SstI	GAGCTC	2 cut(s)	357, 1155
StuI	AGGCCT	1 cut(s)	168
StyD4I	CCNGG	3 cut(s)	222, 897, 1273
StyI	CCWWGG	1 cut(s)	1565
TaaI	ACNGT	2 cut(s)	119, 1316
TaiI	ACGT	1 cut(s)	643
TaqI	TCGA	1 cut(s)	1310
TaqII	GACCGA	1 cut(s)	1492
TasI	AATT	6 cut(s)	334, 840, 963, 1088, 1161, 1250
TatI	WGTACW	1 cut(s)	1316
TauI	GCSGC	3 cut(s)	126, 185, 305
TfiI	GAWTC	3 cut(s)	484, 949, 974
Tru1I	TTAA	4 cut(s)	617, 783, 827, 1373
Tru9I	TTAA	4 cut(s)	617, 783, 827, 1373
TscAI	CASTG	3 cut(s)	730, 932, 1434
TseI	GCWGC	7 cut(s)	244, 257, 633, 710, 986, 1049, 1398
TspDTI	ATGAA	8 cut(s)	243, 446, 489, 768, 821, 956, 1161, 1170
TspGWI	ACGGA	2 cut(s)	70, 1022
TspRI	CASTG	3 cut(s)	730, 932, 1434
Vha464I	CTTAAG	1 cut(s)	616
XapI	RAATTY	2 cut(s)	963, 1250
XcmI	CCANNNNNNNNNTGG	2 cut(s)	334, 1493
XmaJI	CCTAGG	1 cut(s)	1565
XmiI	GTMKAC	1 cut(s)	562
XmnI	GAANNNNTTC	2 cut(s)	396, 498
XspI	CTAG	4 cut(s)	689, 767, 1187, 1566
Zsp2I	ATGCAT	1 cut(s)	1472

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.