MD08G1205900.v1.1

Polysaccharide lyase family 4, domain III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014889
Physical Location & Seq
Reverse (-)
26646923 .. 26652521
5599 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1205900.v1.1.491

Sequence Viewer

Length: 1917 bp
ATGTCATCTGCCGCGGTGCGATTGCATATTCAAGGTCCCCATGTGGTGATGGATAATGGCATACTCCAAGTCACCTTATCGAACCCAGATGGCATTGTCACTGGAATACGATATAATGGCATTGACAATTTGCTTGAGCCTCTTAATAAGGAAACTAACAGAGGTTATTGGGACCTTGTTTGGAGCGCACCAGGAAGTAAGGGAATATTTGATGTGATAAAAGGTACAACTTTTAAGGTTATAGTGGAGAATGAAGAACAGGTTGAGCTCTCGTTTTCAAAAATGTGGGATCACTCTCTTGAGGGGAAATACGTTCCCCTAAATATAGACAAAAGGTTTGTAATGCTTCGTGGTTCCTCGGGCTTCTACTCCTATGCCATTTATGAGCACCTGAAGGATTGGCCTGATTTCGACATTGGTGAAACCAGAATAACCTTCAAACTGAGAAAAGACAAGTTCCAATACATGGCTGTATCAGACAACAGGCAGAGATACATGCCCCTACCTGATGACCGGTTACCAGGACGATGCCAAACCCTTGCCTACCAAGAAGCTGTCCTACTTGTCAATCCCAAGATGAAAGACCTCGCAGGAGAGGTGGATGACAAGTACCAGTATTCTTGTGACAACAAAGATAATAAGGTTCATGGGTGGATATGCACCAACCCACCAGTTGGGTTTTGGCAGATCACGCCCAGTGATGAATTTCGATCTGGTGGACCTCTGAAACAGAACCTAACCTCACATGTCGGCCCTACCACTCTTGCTATGTTTCTTAGTGGTCATTATGCGGGACAAGATCTGGTGCCAAAATTTAGAGGTGGTGAACCATGGAAGAAGGTTTTTGGCCCTGTTTTTATCTATCTCAATTCTTCCACAATCAAAGATGATCCGTTTTGGCTATGGGAGGATGCTAAAATACAGATGATGACAGAAGTACAAAGCTGGCCATATAGTTTCCCAGCATCTGAGGATTTTCAAAAGTCGGATCAACGGGGTAATGTGAGTGGTAGACTACTTGTCTTGGACAGGTACATATGTGAACAATACACACCAGCAGGTGGTGCATATGTTGGTCTTGCCCCACCAGGAGATGTGGGATCATGGCAGAGAGAATGCAAGGACTATCAATTCTGGACAACAGCAGATGACCGTGGCTATTTTGCTATTAACAACGTGCGTACTGGTAATTATAATCTTTATGCATGGGTGCCTGGGTTTATTGGTGATTACCGGTATGATGCTGTCATTGACATAACCTCAGGTTCTTTTATCGAAATGGGTGATCTTGTGTATGAGCCCCCAAGAGATGGGCCAACATTGTGGGAAATAGGCATTCCTGATCGTTCTGCTGCGGAGTTTTATGTTCCTGATCCTGATCCAAAATATGTCAACAAACTTTTCATAAATCATCCTGACAGATTCAGGCAATATGGGCTGTGGAGCAGATATGCTGAAATATATCCTGATGAAGACTTAGTTTACACTATCGGTGTTAGTGATTATGCTAAAGATTGGTTCTTTGCTCAGGTTCCCAGGAAAACAGACAACGGACATGAAGGAACAACATGGCAGATTAAGTTTGAACTGAAAAATGTGGATCGGAACACTACCTATAAATTGCGAGTGGCTATTGCATCTGCAACTCTTGCTGAATTGCAGGTTCGGATTAATGATCCAAATTCAAGGCGACCTCTATTTACTAGTGGATTGATAGGGAGGGATAATTCGGTTGCGAGACTTGGAATTCATGGGATTTACTGGCTGTACAACATGAATGTACCAAGCAATCAGCTTGTTGAGGGGAATAACACTTTCTTCTTCAGACAACCAAGATGCACCAGTCCTTTCCAAGGGATTATGTACGATTATATACGATTTGAAGGTCCCCCGCCTTGTAATTTGAGTTCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

639

Amino Acids

73.08

Weight (kDa)

5.54

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 4 - 200 1e-93 Rhamnogalacturonate lyase family
fn3_3 PF14686 355 - 423 1e-26 Polysaccharide lyase family 4, domain II
CBM-like PF14683 440 - 627 1.8e-51 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014889)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09910 AT1G09910 AT1G09910
fragaria_vesca FvH4_5g34110 FvH4_5g34110
malus_domestica MD08G1205900.v1.1
prunus_persica Prupe.1G538400_v2.0.a1 Prupe.1G538400_v2.0.a1
pyrus_communis pycom08g17810 pycom15g34900
rosa_chinensis RchiOBHm_Chr7g0233611
rosa_laevigata RLG00000001249
rosa_rugosa Rorug07G0281500 Rorug07G0281600
rosa_samantha Rh7AG438100 Rh7BG410800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1194
AarI CACCTGC 1 cut(s) 1049
Acc36I ACCTGC 2 cut(s) 1049, 1651
AccB1I GGYRCC 2 cut(s) 805, 1210
AccB7I CCANNNNNTGG 4 cut(s) 466, 674, 1061, 1310
AccI GTMKAC 1 cut(s) 1012
AccII CGCG 1 cut(s) 14
AciI CCGC 5 cut(s) 12, 14, 791, 1355, 1892
AclWI GGATC 8 cut(s) 297, 884, 996, 1108, 1367, 1373, 1608, 1670
AcoI YGGCCR 1 cut(s) 947
AcsI RAATTY 4 cut(s) 704, 812, 1681, 1746
AcuI CTGAAG 2 cut(s) 413, 1807
AfaI GTAC 8 cut(s) 226, 611, 939, 1034, 1183, 1769, 1782, 1865
AfiI CCNNNNNNNGG 5 cut(s) 466, 674, 1061, 1310, 1853
AflIII ACRYGT 1 cut(s) 745
AgeI ACCGGT 2 cut(s) 513, 1233
AgsI TTSAA 7 cut(s) 32, 279, 439, 980, 1586, 1686, 1883
AhdI GACNNNNNGTC 1 cut(s) 1019
AhlI ACTAGT 1 cut(s) 1703
AjnI CCWGG 5 cut(s) 190, 520, 1087, 1213, 1535
AluBI AGCT 4 cut(s) 268, 554, 945, 1795
AluI AGCT 4 cut(s) 268, 554, 945, 1795
Alw21I GWGCWC 2 cut(s) 270, 390
Alw26I GTCTC 1 cut(s) 1732
AlwI GGATC 8 cut(s) 297, 884, 996, 1108, 1367, 1373, 1608, 1670
AlwNI CAGNNNCTG 1 cut(s) 968
Ama87I CYCGRG 1 cut(s) 358
AoxI GGCC 5 cut(s) 401, 751, 847, 947, 1313
ApeKI GCWGC 1 cut(s) 1352
ApoI RAATTY 4 cut(s) 704, 812, 1681, 1746
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
AseI ATTAAT 1 cut(s) 1671
AsiGI ACCGGT 2 cut(s) 513, 1233
AspLEI GCGC 1 cut(s) 188
AspS9I GGNCC 7 cut(s) 35, 172, 719, 752, 848, 1313, 1886
AsuHPI GGTGA 6 cut(s) 58, 64, 431, 836, 1238, 1295
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 4 cut(s) 35, 172, 719, 1886
AxyI CCTNAGG 1 cut(s) 1261
BaeI ACNNNNGTAYC 2 cut(s) 1024, 1057
BalI TGGCCA 1 cut(s) 949
BanI GGYRCC 2 cut(s) 805, 1210
BanII GRGCYC 2 cut(s) 270, 1302
BarI GAAGNNNNNNTAC 2 cut(s) 543, 575
BbsI GAAGAC 1 cut(s) 1479
Bbv12I GWGCWC 2 cut(s) 270, 390
BbvI GCAGC 1 cut(s) 1339
BccI CCATC 3 cut(s) 43, 83, 1304
BciT130I CCWGG 5 cut(s) 192, 522, 1089, 1215, 1537
BcoDI GTCTC 1 cut(s) 1732
BcuI ACTAGT 1 cut(s) 1703
BfaI CTAG 1 cut(s) 1704
BfuAI ACCTGC 2 cut(s) 1049, 1651
BglII AGATCT 1 cut(s) 799
BisI GCNGC 2 cut(s) 12, 1353
BlsI GCNGC 2 cut(s) 13, 1354
Bme1390I CCNGG 5 cut(s) 192, 522, 1089, 1215, 1537
Bme18I GGWCC 4 cut(s) 35, 172, 719, 1886
BmeRI GACNNNNNGTC 1 cut(s) 1019
BmeT110I CYCGRG 1 cut(s) 358
BmgT120I GGNCC 7 cut(s) 35, 172, 719, 752, 848, 1313, 1886
BmiI GGNNCC 7 cut(s) 37, 173, 355, 807, 1212, 1533, 1888
BmrFI CCNGG 5 cut(s) 192, 522, 1089, 1215, 1537
BmrI ACTGGG 1 cut(s) 690
BmsI GCATC 6 cut(s) 518, 901, 974, 1231, 1646, 1826
BmuI ACTGGG 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 1479
Bpu10I CCTNAGC 1 cut(s) 1527
BpuEI CTTGAG 2 cut(s) 155, 320
BsaBI GATNNNNATC 1 cut(s) 1377
BsaJI CCNNGG 7 cut(s) 12, 357, 830, 1153, 1214, 1535, 1852
BsaWI WCCGGW 2 cut(s) 513, 1233
Bsc4I CCNNNNNNNGG 5 cut(s) 466, 674, 1061, 1310, 1853
Bse118I RCCGGY 2 cut(s) 513, 1233
Bse1I ACTGG 7 cut(s) 106, 613, 671, 696, 1189, 1766, 1842
Bse21I CCTNAGG 1 cut(s) 1261
Bse8I GATNNNNATC 1 cut(s) 1377
BseBI CCWGG 5 cut(s) 192, 522, 1089, 1215, 1537
BseDI CCNNGG 7 cut(s) 12, 357, 830, 1153, 1214, 1535, 1852
BseGI GGATG 3 cut(s) 607, 916, 1411
BseJI GATNNNNATC 1 cut(s) 1377
BseLI CCNNNNNNNGG 5 cut(s) 466, 674, 1061, 1310, 1853
BseMII CTCAG 4 cut(s) 434, 960, 1275, 1541
BseNI ACTGG 7 cut(s) 106, 613, 671, 696, 1189, 1766, 1842
BseXI GCAGC 1 cut(s) 1339
BseYI CCCAGC 1 cut(s) 961
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 5 cut(s) 403, 753, 849, 949, 1315
BshNI GGYRCC 2 cut(s) 805, 1210
BshTI ACCGGT 2 cut(s) 513, 1233
BsiHKAI GWGCWC 2 cut(s) 270, 390
BsiHKCI CYCGRG 1 cut(s) 358
BsiSI CCGG 2 cut(s) 514, 1234
BslFI GGGAC 4 cut(s) 21, 185, 807, 1872
BslI CCNNNNNNNGG 5 cut(s) 466, 674, 1061, 1310, 1853
BsmAI GTCTC 1 cut(s) 1732
BsmFI GGGAC 4 cut(s) 21, 185, 807, 1872
BsmI GAATGC 2 cut(s) 1121, 1335
BsnI GGCC 5 cut(s) 403, 753, 849, 949, 1315
BsoBI CYCGRG 1 cut(s) 358
Bsp1286I GDGCHC 3 cut(s) 270, 390, 1302
Bsp1407I TGTACA 1 cut(s) 1767
Bsp19I CCATGG 1 cut(s) 830
BspACI CCGC 5 cut(s) 12, 14, 791, 1355, 1892
BspANI GGCC 5 cut(s) 403, 753, 849, 949, 1315
BspCNI CTCAG 4 cut(s) 435, 961, 1274, 1540
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 7 cut(s) 37, 173, 355, 807, 1212, 1533, 1888
BspMI ACCTGC 2 cut(s) 1049, 1651
BspPI GGATC 8 cut(s) 297, 884, 996, 1108, 1367, 1373, 1608, 1670
BspT107I GGYRCC 2 cut(s) 805, 1210
BsrFI RCCGGY 2 cut(s) 513, 1233
BsrGI TGTACA 1 cut(s) 1767
BsrI ACTGG 7 cut(s) 106, 613, 671, 696, 1189, 1766, 1842
BssAI RCCGGY 2 cut(s) 513, 1233
BssECI CCNNGG 7 cut(s) 12, 357, 830, 1153, 1214, 1535, 1852
BssT1I CCWWGG 2 cut(s) 830, 1852
Bst2UI CCWGG 5 cut(s) 192, 522, 1089, 1215, 1537
Bst4CI ACNGT 1 cut(s) 1154
BstAPI GCANNNNNTGC 2 cut(s) 1064, 1649
BstAUI TGTACA 1 cut(s) 1767
BstC8I GCNNGC 1 cut(s) 947
BstDEI CTNAG 7 cut(s) 443, 776, 969, 1261, 1477, 1527, 1914
BstDSI CCRYGG 3 cut(s) 12, 830, 1153
BstEII GGTNACC 1 cut(s) 516
BstENI CCTNNNNNAGG 1 cut(s) 1851
BstF5I GGATG 3 cut(s) 607, 916, 1411
BstFNI CGCG 1 cut(s) 14
BstHHI GCGC 1 cut(s) 188
BstMAI GTCTC 1 cut(s) 1732
BstMWI GCNNNNNNNGC 4 cut(s) 691, 1064, 1435, 1649
BstNI CCWGG 5 cut(s) 192, 522, 1089, 1215, 1537
BstNSI RCATGY 2 cut(s) 499, 749
BstPI GGTNACC 1 cut(s) 516
BstSCI CCNGG 5 cut(s) 190, 520, 1087, 1213, 1535
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 1339
BstV2I GAAGAC 1 cut(s) 1479
BstX2I RGATCY 1 cut(s) 799
BstXI CCANNNNNNTGG 1 cut(s) 1323
BstYI RGATCY 1 cut(s) 799
Bsu36I CCTNAGG 1 cut(s) 1261
BsuRI GGCC 5 cut(s) 403, 753, 849, 949, 1315
BtgI CCRYGG 3 cut(s) 12, 830, 1153
BtsCI GGATG 3 cut(s) 607, 916, 1411
BtsIMutI CAGTG 2 cut(s) 99, 703
BveI ACCTGC 2 cut(s) 1049, 1651
Cac8I GCNNGC 1 cut(s) 947
CaiI CAGNNNCTG 1 cut(s) 968
CfoI GCGC 1 cut(s) 188
Cfr10I RCCGGY 2 cut(s) 513, 1233
Cfr13I GGNCC 7 cut(s) 35, 172, 719, 752, 848, 1313, 1886
Cfr42I CCGCGG 1 cut(s) 15
Csp6I GTAC 8 cut(s) 225, 610, 938, 1033, 1182, 1768, 1781, 1864
CspAI ACCGGT 2 cut(s) 513, 1233
CviQI GTAC 8 cut(s) 225, 610, 938, 1033, 1182, 1768, 1781, 1864
DdeI CTNAG 7 cut(s) 443, 776, 969, 1261, 1477, 1527, 1914
DriI GACNNNNNGTC 1 cut(s) 1019
EaeI YGGCCR 1 cut(s) 947
Eam1105I GACNNNNNGTC 1 cut(s) 1019
Ecl136II GAGCTC 1 cut(s) 268
Eco130I CCWWGG 2 cut(s) 830, 1852
Eco24I GRGCYC 2 cut(s) 270, 1302
Eco47I GGWCC 4 cut(s) 35, 172, 719, 1886
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 2 cut(s) 413, 1807
Eco81I CCTNAGG 1 cut(s) 1261
Eco88I CYCGRG 1 cut(s) 358
Eco91I GGTNACC 1 cut(s) 516
EcoICRI GAGCTC 1 cut(s) 268
EcoNI CCTNNNNNAGG 1 cut(s) 1851
EcoO109I RGGNCCY 3 cut(s) 35, 172, 1886
EcoO65I GGTNACC 1 cut(s) 516
EcoRI GAATTC 1 cut(s) 1746
EcoRII CCWGG 5 cut(s) 190, 520, 1087, 1213, 1535
EcoT14I CCWWGG 2 cut(s) 830, 1852
EcoT22I ATGCAT 1 cut(s) 1207
EcoT38I GRGCYC 2 cut(s) 270, 1302
ErhI CCWWGG 2 cut(s) 830, 1852
FaqI GGGAC 4 cut(s) 21, 185, 807, 1872
FauI CCCGC 2 cut(s) 784, 1899
FauNDI CATATG 2 cut(s) 1037, 1069
FblI GTMKAC 1 cut(s) 1012
Fnu4HI GCNGC 2 cut(s) 12, 1353
FokI GGATG 3 cut(s) 614, 923, 1398
FriOI GRGCYC 2 cut(s) 270, 1302
Fsp4HI GCNGC 2 cut(s) 12, 1353
FspBI CTAG 1 cut(s) 1704
GlaI GCGC 1 cut(s) 187
GluI GCNGC 2 cut(s) 12, 1353
GsaI CCCAGC 1 cut(s) 965
HaeIII GGCC 5 cut(s) 403, 753, 849, 949, 1315
HapII CCGG 2 cut(s) 514, 1234
HhaI GCGC 1 cut(s) 188
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HincII GTYRAC 1 cut(s) 1393
HindII GTYRAC 1 cut(s) 1393
HinfI GANTC 1 cut(s) 1422
HpaII CCGG 2 cut(s) 514, 1234
HphI GGTGA 6 cut(s) 58, 64, 431, 836, 1238, 1295
Hpy166II GTNNAC 6 cut(s) 719, 827, 1013, 1043, 1393, 1483
Hpy188I TCNGA 7 cut(s) 478, 726, 970, 988, 1605, 1668, 1826
Hpy188III TCNNGA 7 cut(s) 299, 1135, 1340, 1370, 1376, 1415, 1466
Hpy8I GTNNAC 6 cut(s) 719, 827, 1013, 1043, 1393, 1483
HpyAV CCTTC 5 cut(s) 388, 445, 832, 1553, 1877
HpyCH4III ACNGT 1 cut(s) 1154
HpyCH4IV ACGT 2 cut(s) 312, 1176
HpyCH4V TGCA 9 cut(s) 25, 660, 1067, 1119, 1205, 1637, 1643, 1660, 1839
HpyF10VI GCNNNNNNNGC 4 cut(s) 691, 1064, 1435, 1649
HpyF3I CTNAG 7 cut(s) 443, 776, 969, 1261, 1477, 1527, 1914
HpySE526I ACGT 2 cut(s) 312, 1176
HspAI GCGC 1 cut(s) 186
KspI CCGCGG 1 cut(s) 15
LmnI GCTCC 2 cut(s) 183, 1443
Lsp1109I GCAGC 1 cut(s) 1339
LweI GCATC 6 cut(s) 518, 901, 974, 1231, 1646, 1826
MaeI CTAG 1 cut(s) 1704
MaeII ACGT 2 cut(s) 312, 1176
MaeIII GTNAC 4 cut(s) 70, 97, 516, 623
MboII GAAGA 6 cut(s) 266, 847, 864, 1484, 1810, 1813
MflI RGATCY 1 cut(s) 799
MhlI GDGCHC 3 cut(s) 270, 390, 1302
MlsI TGGCCA 1 cut(s) 949
MluNI TGGCCA 1 cut(s) 949
MmeI TCCRAC 1 cut(s) 966
Mox20I TGGCCA 1 cut(s) 949
Mph1103I ATGCAT 1 cut(s) 1207
MscI TGGCCA 1 cut(s) 949
MseI TTAA 5 cut(s) 144, 234, 1170, 1578, 1671
Msp20I TGGCCA 1 cut(s) 949
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 2 cut(s) 514, 1234
MspR9I CCNGG 5 cut(s) 192, 522, 1089, 1215, 1537
Mva1269I GAATGC 2 cut(s) 1121, 1335
MvaI CCWGG 5 cut(s) 192, 522, 1089, 1215, 1537
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 4 cut(s) 691, 1064, 1435, 1649
NcoI CCATGG 1 cut(s) 830
NdeI CATATG 2 cut(s) 1037, 1069
NlaIV GGNNCC 7 cut(s) 37, 173, 355, 807, 1212, 1533, 1888
NmuCI GTSAC 3 cut(s) 70, 97, 623
NsiI ATGCAT 1 cut(s) 1207
NspI RCATGY 2 cut(s) 499, 749
PaqCI CACCTGC 1 cut(s) 1049
PciI ACATGT 1 cut(s) 745
PctI GAATGC 2 cut(s) 1121, 1335
PfeI GAWTC 1 cut(s) 1422
PflMI CCANNNNNTGG 4 cut(s) 466, 674, 1061, 1310
PinAI ACCGGT 2 cut(s) 513, 1233
PkrI GCNGC 2 cut(s) 13, 1354
PpuMI RGGWCCY 3 cut(s) 35, 172, 1886
PscI ACATGT 1 cut(s) 745
PshBI ATTAAT 1 cut(s) 1671
PsiI TTATAA 1 cut(s) 1194
Psp124BI GAGCTC 1 cut(s) 270
Psp5II RGGWCCY 3 cut(s) 35, 172, 1886
Psp6I CCWGG 5 cut(s) 190, 520, 1087, 1213, 1535
PspEI GGTNACC 1 cut(s) 516
PspFI CCCAGC 1 cut(s) 961
PspGI CCWGG 5 cut(s) 190, 520, 1087, 1213, 1535
PspN4I GGNNCC 7 cut(s) 37, 173, 355, 807, 1212, 1533, 1888
PspPI GGNCC 7 cut(s) 35, 172, 719, 752, 848, 1313, 1886
PspPPI RGGWCCY 3 cut(s) 35, 172, 1886
PsrI GAACNNNNNNTAC 1 cut(s) 1891
PstNI CAGNNNCTG 1 cut(s) 968
PsuI RGATCY 1 cut(s) 799
RsaI GTAC 8 cut(s) 226, 611, 939, 1034, 1183, 1769, 1782, 1865
RsaNI GTAC 8 cut(s) 225, 610, 938, 1033, 1182, 1768, 1781, 1864
SacI GAGCTC 1 cut(s) 270
SacII CCGCGG 1 cut(s) 15
SaqAI TTAA 5 cut(s) 144, 234, 1170, 1578, 1671
SatI GCNGC 2 cut(s) 12, 1353
Sau96I GGNCC 7 cut(s) 35, 172, 719, 752, 848, 1313, 1886
ScrFI CCNGG 5 cut(s) 192, 522, 1089, 1215, 1537
SduI GDGCHC 3 cut(s) 270, 390, 1302
SfaNI GCATC 6 cut(s) 518, 901, 974, 1231, 1646, 1826
Sfr303I CCGCGG 1 cut(s) 15
SgrBI CCGCGG 1 cut(s) 15
SinI GGWCC 4 cut(s) 35, 172, 719, 1886
SmlI CTYRAG 2 cut(s) 134, 299
SmoI CTYRAG 2 cut(s) 134, 299
SpeI ACTAGT 1 cut(s) 1703
SsiI CCGC 5 cut(s) 12, 14, 791, 1355, 1892
SspI AATATT 1 cut(s) 207
SspMI CTAG 1 cut(s) 1704
SstI GAGCTC 1 cut(s) 270
StyD4I CCNGG 5 cut(s) 190, 520, 1087, 1213, 1535
StyI CCWWGG 2 cut(s) 830, 1852
TaaI ACNGT 1 cut(s) 1154
TaiI ACGT 2 cut(s) 315, 1179
TaqI TCGA 4 cut(s) 80, 411, 709, 1275
TatI WGTACW 2 cut(s) 937, 1767
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 1 cut(s) 1422
Tru1I TTAA 5 cut(s) 144, 234, 1170, 1578, 1671
Tru9I TTAA 5 cut(s) 144, 234, 1170, 1578, 1671
TscAI CASTG 2 cut(s) 106, 703
TseFI GTSAC 3 cut(s) 70, 97, 623
TseI GCWGC 1 cut(s) 1352
Tsp45I GTSAC 3 cut(s) 70, 97, 623
TspGWI ACGGA 2 cut(s) 882, 1566
TspRI CASTG 2 cut(s) 106, 703
Van91I CCANNNNNTGG 4 cut(s) 466, 674, 1061, 1310
VpaK11BI GGWCC 4 cut(s) 35, 172, 719, 1886
VspI ATTAAT 1 cut(s) 1671
XagI CCTNNNNNAGG 1 cut(s) 1851
XapI RAATTY 4 cut(s) 704, 812, 1681, 1746
XceI RCATGY 2 cut(s) 499, 749
XcmI CCANNNNNNNNNTGG 1 cut(s) 678
XmiI GTMKAC 1 cut(s) 1012
XspI CTAG 1 cut(s) 1704
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.