Rorug07G0281500

Polysaccharide lyase family 4, domain III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0014889
Physical Location & Seq
Reverse (-)
27311803 .. 27318461
6659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0281500.1

Sequence Viewer

Length: 1962 bp
ATGTACAAGAGAGTGTGGCAAAAAGATTTTGTTATGAACTTGCTCAGATGTGTCACATTTCTGGCATGGCGTTCAATGCAGAACCTGTACGTGCTTCCACCATTCAGTGCTCGACCTAATCAGGTGGAGAAGGTCTTAAAAACTCAGTATCATGATGTGATGACTAAGATTCCGGGCAAGAAGCTTGACTTACTTGTTGTCATCTTGCCAAATAATAATGGCTCTCTTTATGGTGATTTAAAAAGGATTTGTGAGACAGAGCTTGGCCTGGTATCCCAGTGCTGTTTAACTCAACATGTGTTTAAAATGAATAAACAGTATTTAGCAAACATTGTCTTGAAGATAAATGTGAAGGTTGGGGGACGGAACACAGTACTTGTTGATGCAATCTCCAGACGTATTCCCTTGGTTAGCAACCATCCTACAATAATTTTCGGTGCTGGTATGACGCATCCTCATCCAGGGGAGAATTCAAGCCCATCAATTGCAGCTGTTGTGGCTTCTCAAGACTGGCCTGAAATTACCAAGTATGCTGGTTTGGTTTGTGCTCAAGCCCATCGTCAGGAGCTCATCCAAGATTTGTTCAAACAGTGGCAGGATCCAGTAGAAGGGACAATGTCTGGTGGAATGATAAAGGAACTGCTTATTTCATTTTATGGAGCGACAGGGCAGAAGCCTCAACGCATCACTACAGGCATTTTCTACCTTCTTTGGCATTTTCTCATCCTACGTACTCCTACTGCTTATCCGGCGTCGCCACCGTCGAGCACGTCCCGCTCAGCCGACGGTAGGGTCGAGTTTGCCTTTGGTTGTGCTCCTCCAATCCCTCAGTACCTCCCACGCGCCGCCCAGCGTCGGCTACGTCCTTACCAGTTCATCCTCTGCCTCGGCTCCGTCGACCGCGCCACCGACTCCACTCTAGTTGCCGACCTCGGTTGTCAATCTTTGACGCATTTCGACGGCCAAATTTCCGGTGTTACGGCTACGAAGGTGGTTTTGGTACGAGAGGATTTCTTTGCGAGGACGTTGACATTGAACAGGCCTCGGCAACTGAATGCCCTTAACTTTGAAATGTCTCTGCGTCACCTCCCTAGATGGAGCTCGGAAACTGTATCCACTAATGTCAATTGGGTTGTTGTGGGCACAACGTTTCAAGTCTCTGTACACAGTGGCAGTGCCAGAATGCCATTGACAATTGCACATAAAATTGCTAGCTTGGAGTCTCTACAAGAGATCCTTATGAGGCATTATACTCGCAACTTCGTCATGAGATGTTTAAGGGACTCAGAGGTCTTCTTCAACCACCGATCAATGGAGTTGAGAATCATGGTCTTCCTGTTGTGCTCTAAGTATCTATCGTGCAGGCGTAGCTTTAGATATTACAGGAAAAGTGTCAGCGAGGGGCAGTTCTCCCAAGTTCTACTGTACGAACTTGATGCAATTCGTAAGGCCTGCGCTTCACTGGAGCCAAATTATCAGCCTCCTGTGACTTTTGTTGTTGTTCAAAAGAGTCATCGTACAAGACTGTTTGCCAACAACCATGGTGATCGCCGTGCAGTTGACGGGAGTGGGAACATATTACCTGGTACGGTTGTGGACTCCAAAATCTGTCACCCAACTGAGTTTGACTTCTACCTCTGTAGCCATGCAGGAATTCAGGGTACAAGCCGCCCAGCTCATTACCATGTGTTGTGGGATGAAAATAAGTTCACCGCAGACGCACTGCAGTCCCTTACCAATAATCTTTGCTATACATATGCAAGGTGTACACGCTCAGTTTCAATCGTGCCTCCTGCTTATTATGCTCATCTCGCCGCGACCCGAGCTCGGTTCTATCTGGAGCCCGTGACTTCAGACAGTGGTTCAATGTCAAGTGGTATGGGTGGCTCTGGTGGAGGACGGAACTCTCGAGCACCAGGTCTAAAAGCTTCTGTGAGGCCTAAAGGCCTTTGCCTGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

653

Amino Acids

73.31

Weight (kDa)

9.63

Isoelectric Point (pI)

51.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Piwi PF02171 65 - 230 5.6e-38 Piwi domain
Piwi PF02171 460 - 612 8.6e-62 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014889)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09910 AT1G09910 AT1G09910
fragaria_vesca FvH4_5g34110 FvH4_5g34110
malus_domestica MD08G1205900.v1.1
prunus_persica Prupe.1G538400_v2.0.a1 Prupe.1G538400_v2.0.a1
pyrus_communis pycom08g17810 pycom15g34900
rosa_chinensis RchiOBHm_Chr7g0233611
rosa_laevigata RLG00000001249
rosa_rugosa Rorug07G0281500 Rorug07G0281600
rosa_samantha Rh7AG438100 Rh7BG410800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 791, 1289
AccBSI CCGCTC 1 cut(s) 777
AccI GTMKAC 1 cut(s) 897
AccII CGCG 3 cut(s) 843, 903, 1817
AciI CCGC 6 cut(s) 775, 846, 901, 1669, 1713, 1815
AclI AACGTT 1 cut(s) 1148
AclWI GGATC 3 cut(s) 593, 606, 1228
AcoI YGGCCR 1 cut(s) 961
AcsI RAATTY 3 cut(s) 469, 966, 1653
AcuI CTGAAG 1 cut(s) 1836
AcyI GRCGYC 1 cut(s) 752
AfiI CCNNNNNNNGG 7 cut(s) 461, 562, 608, 789, 855, 1312, 1827
AflIII ACRYGT 1 cut(s) 295
AjiI CACGTC 1 cut(s) 771
AjnI CCWGG 4 cut(s) 267, 460, 1582, 1915
AjuI GAANNNNNNNTTGG 2 cut(s) 980, 1012
Alw21I GWGCWC 9 cut(s) 112, 550, 570, 770, 817, 1103, 1346, 1828, 1915
Alw26I GTCTC 4 cut(s) 248, 1080, 1162, 1227
AlwI GGATC 3 cut(s) 593, 606, 1228
AlwNI CAGNNNCTG 1 cut(s) 85
Ama87I CYCGRG 2 cut(s) 1821, 1908
AoxI GGCC 7 cut(s) 265, 512, 961, 1040, 1449, 1937, 1945
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 3 cut(s) 469, 966, 1653
AspLEI GCGC 3 cut(s) 845, 905, 1457
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 5 cut(s) 245, 1076, 1556, 1604, 1702
AsuNHI GCTAGC 1 cut(s) 1211
AvaI CYCGRG 2 cut(s) 1821, 1908
BaeGI GKGCMC 1 cut(s) 1145
BaeI ACNNNNGTAYC 2 cut(s) 1578, 1611
BamHI GGATCC 1 cut(s) 598
BanII GRGCYC 4 cut(s) 570, 1103, 1828, 1845
BbsI GAAGAC 2 cut(s) 1285, 1324
Bbv12I GWGCWC 9 cut(s) 112, 550, 570, 770, 817, 1103, 1346, 1828, 1915
BbvI GCAGC 1 cut(s) 500
BccI CCATC 4 cut(s) 426, 487, 564, 1089
BceAI ACGGC 3 cut(s) 976, 996, 1536
BcgI CGANNNNNNTGC 4 cut(s) 1235, 1269, 1418, 1452
BciT130I CCWGG 4 cut(s) 269, 462, 1584, 1917
BciVI GTATCC 2 cut(s) 283, 1123
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 4 cut(s) 248, 1080, 1162, 1227
BfaI CTAG 3 cut(s) 920, 1092, 1212
BfmI CTRYAG 3 cut(s) 690, 1639, 1724
BfuI GTATCC 2 cut(s) 283, 1123
BisI GCNGC 4 cut(s) 489, 846, 1669, 1815
BlpI GCTNAGC 1 cut(s) 778
BlsI GCNGC 4 cut(s) 490, 847, 1670, 1816
BmcAI AGTACT 1 cut(s) 375
Bme1390I CCNGG 5 cut(s) 174, 269, 462, 1584, 1917
BmeT110I CYCGRG 2 cut(s) 1821, 1908
BmgBI CACGTC 1 cut(s) 771
BmiI GGNNCC 4 cut(s) 600, 892, 1467, 1842
BmrFI CCNGG 5 cut(s) 174, 269, 462, 1584, 1917
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 4 cut(s) 373, 460, 693, 1426
BmtI GCTAGC 1 cut(s) 1215
BmuI ACTGGG 1 cut(s) 271
BpiI GAAGAC 2 cut(s) 1285, 1324
BpmI CTGGAG 3 cut(s) 376, 1484, 1859
Bpu1102I GCTNAGC 1 cut(s) 778
BpuEI CTTGAG 2 cut(s) 489, 534
BpuMI CCSGG 1 cut(s) 174
BsaAI YACGTR 2 cut(s) 91, 731
BsaHI GRCGYC 1 cut(s) 752
BsaJI CCNNGG 6 cut(s) 405, 461, 886, 931, 1043, 1540
BsaWI WCCGGW 1 cut(s) 971
Bsc4I CCNNNNNNNGG 7 cut(s) 461, 562, 608, 789, 855, 1312, 1827
Bse1I ACTGG 5 cut(s) 277, 515, 602, 871, 1467
BseBI CCWGG 4 cut(s) 269, 462, 1584, 1917
BseDI CCNNGG 6 cut(s) 405, 461, 886, 931, 1043, 1540
BseGI GGATG 7 cut(s) 418, 451, 457, 570, 723, 876, 1702
BseLI CCNNNNNNNGG 7 cut(s) 461, 562, 608, 789, 855, 1312, 1827
BseMII CTCAG 7 cut(s) 58, 158, 792, 842, 1299, 1611, 1788
BseNI ACTGG 5 cut(s) 277, 515, 602, 871, 1467
BseRI GAGGAG 1 cut(s) 807
BseSI GKGCMC 1 cut(s) 1145
BseXI GCAGC 1 cut(s) 500
BseYI CCCAGC 2 cut(s) 849, 1672
BsgI GTGCAG 2 cut(s) 1381, 1575
Bsh1236I CGCG 3 cut(s) 843, 903, 1817
Bsh1285I CGRYCG 1 cut(s) 901
BshFI GGCC 7 cut(s) 267, 514, 963, 1042, 1451, 1939, 1947
BsiEI CGRYCG 1 cut(s) 901
BsiHKAI GWGCWC 9 cut(s) 112, 550, 570, 770, 817, 1103, 1346, 1828, 1915
BsiHKCI CYCGRG 2 cut(s) 1821, 1908
BsiSI CCGG 3 cut(s) 173, 749, 972
BslFI GGGAC 5 cut(s) 375, 625, 757, 1295, 1714
BslI CCNNNNNNNGG 7 cut(s) 461, 562, 608, 789, 855, 1312, 1827
BsmAI GTCTC 4 cut(s) 248, 1080, 1162, 1227
BsmFI GGGAC 5 cut(s) 375, 625, 757, 1295, 1714
BsmI GAATGC 2 cut(s) 1060, 1188
BsnI GGCC 7 cut(s) 267, 514, 963, 1042, 1451, 1939, 1947
BsoBI CYCGRG 2 cut(s) 1821, 1908
Bsp1407I TGTACA 3 cut(s) 3, 1162, 1766
Bsp143I GATC 4 cut(s) 598, 1233, 1307, 1546
Bsp1720I GCTNAGC 1 cut(s) 778
Bsp19I CCATGG 1 cut(s) 1540
BspACI CCGC 6 cut(s) 775, 846, 901, 1669, 1713, 1815
BspANI GGCC 7 cut(s) 267, 514, 963, 1042, 1451, 1939, 1947
BspCNI CTCAG 7 cut(s) 57, 157, 791, 841, 1298, 1612, 1787
BspFNI CGCG 3 cut(s) 843, 903, 1817
BspHI TCATGA 2 cut(s) 151, 1266
BspLI GGNNCC 4 cut(s) 600, 892, 1467, 1842
BspMAI CTGCAG 1 cut(s) 1728
BspOI GCTAGC 1 cut(s) 1215
BspPI GGATC 3 cut(s) 593, 606, 1228
BsrBI CCGCTC 1 cut(s) 777
BsrGI TGTACA 3 cut(s) 3, 1162, 1766
BsrI ACTGG 5 cut(s) 277, 515, 602, 871, 1467
BssECI CCNNGG 6 cut(s) 405, 461, 886, 931, 1043, 1540
BssMI GATC 4 cut(s) 598, 1233, 1307, 1546
BssNI GRCGYC 1 cut(s) 752
BssT1I CCWWGG 2 cut(s) 405, 1540
Bst2UI CCWGG 4 cut(s) 269, 462, 1584, 1917
BstACI GRCGYC 1 cut(s) 752
BstAUI TGTACA 3 cut(s) 3, 1162, 1766
BstBAI YACGTR 2 cut(s) 91, 731
BstC8I GCNNGC 4 cut(s) 1213, 1364, 1453, 1955
BstDEI CTNAG 9 cut(s) 44, 144, 165, 778, 828, 1285, 1347, 1620, 1774
BstDSI CCRYGG 1 cut(s) 1540
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 7 cut(s) 418, 451, 457, 570, 723, 876, 1702
BstFNI CGCG 3 cut(s) 843, 903, 1817
BstHHI GCGC 3 cut(s) 845, 905, 1457
BstKTI GATC 4 cut(s) 601, 1236, 1310, 1549
BstMAI GTCTC 4 cut(s) 248, 1080, 1162, 1227
BstMBI GATC 4 cut(s) 598, 1233, 1307, 1546
BstMCI CGRYCG 1 cut(s) 901
BstMWI GCNNNNNNNGC 8 cut(s) 76, 497, 749, 774, 1368, 1802, 1811, 1823
BstNI CCWGG 4 cut(s) 269, 462, 1584, 1917
BstNSI RCATGY 1 cut(s) 299
BstSCI CCNGG 5 cut(s) 172, 267, 460, 1582, 1915
BstSFI CTRYAG 3 cut(s) 690, 1639, 1724
BstSLI GKGCMC 1 cut(s) 1145
BstSNI TACGTA 1 cut(s) 731
BstUI CGCG 3 cut(s) 843, 903, 1817
BstV1I GCAGC 1 cut(s) 500
BstV2I GAAGAC 2 cut(s) 1285, 1324
BstX2I RGATCY 2 cut(s) 598, 1233
BstYI RGATCY 2 cut(s) 598, 1233
BsuI GTATCC 2 cut(s) 283, 1123
BsuRI GGCC 7 cut(s) 267, 514, 963, 1042, 1451, 1939, 1947
BtgI CCRYGG 1 cut(s) 1540
BtrI CACGTC 1 cut(s) 771
BtsCI GGATG 7 cut(s) 418, 451, 457, 570, 723, 876, 1702
BtsI GCAGTG 2 cut(s) 1180, 1721
BtsIMutI CAGTG 8 cut(s) 112, 284, 596, 1174, 1180, 1460, 1721, 1864
Cac8I GCNNGC 4 cut(s) 1213, 1364, 1453, 1955
CaiI CAGNNNCTG 1 cut(s) 85
CciI TCATGA 2 cut(s) 151, 1266
CfoI GCGC 3 cut(s) 845, 905, 1457
CseI GACGC 6 cut(s) 457, 741, 842, 958, 1070, 1727
CsiI ACCWGGT 2 cut(s) 1582, 1915
CspCI CAANNNNNGTGG 2 cut(s) 904, 939
CviAII CATG 8 cut(s) 66, 152, 296, 1267, 1327, 1541, 1646, 1685
DdeI CTNAG 9 cut(s) 44, 144, 165, 778, 828, 1285, 1347, 1620, 1774
DpnI GATC 4 cut(s) 600, 1235, 1309, 1548
DpnII GATC 4 cut(s) 598, 1233, 1307, 1546
DraI TTTAAA 2 cut(s) 240, 304
DrdI GACNNNNNNGTC 2 cut(s) 791, 1289
DseDI GACNNNNNNGTC 2 cut(s) 791, 1289
EaeI YGGCCR 1 cut(s) 961
Ecl136II GAGCTC 3 cut(s) 568, 1101, 1826
Eco105I TACGTA 1 cut(s) 731
Eco130I CCWWGG 2 cut(s) 405, 1540
Eco147I AGGCCT 4 cut(s) 1042, 1451, 1939, 1947
Eco24I GRGCYC 4 cut(s) 570, 1103, 1828, 1845
Eco53kI GAGCTC 3 cut(s) 568, 1101, 1826
Eco57I CTGAAG 1 cut(s) 1836
Eco88I CYCGRG 2 cut(s) 1821, 1908
EcoICRI GAGCTC 3 cut(s) 568, 1101, 1826
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoRI GAATTC 2 cut(s) 469, 1653
EcoRII CCWGG 4 cut(s) 267, 460, 1582, 1915
EcoT14I CCWWGG 2 cut(s) 405, 1540
EcoT38I GRGCYC 4 cut(s) 570, 1103, 1828, 1845
ErhI CCWWGG 2 cut(s) 405, 1540
FaeI CATG 8 cut(s) 69, 155, 299, 1270, 1330, 1544, 1649, 1688
FalI AAGNNNNNCTT 4 cut(s) 173, 205, 1221, 1253
FaqI GGGAC 5 cut(s) 375, 625, 757, 1295, 1714
FatI CATG 8 cut(s) 65, 151, 295, 1266, 1326, 1540, 1645, 1684
FauI CCCGC 1 cut(s) 782
FauNDI CATATG 1 cut(s) 1756
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 4 cut(s) 489, 846, 1669, 1815
FokI GGATG 7 cut(s) 405, 438, 444, 557, 710, 863, 1709
FriOI GRGCYC 4 cut(s) 570, 1103, 1828, 1845
Fsp4HI GCNGC 4 cut(s) 489, 846, 1669, 1815
FspBI CTAG 3 cut(s) 920, 1092, 1212
GlaI GCGC 3 cut(s) 844, 904, 1456
GluI GCNGC 4 cut(s) 489, 846, 1669, 1815
GsaI CCCAGC 2 cut(s) 853, 1676
GsuI CTGGAG 3 cut(s) 376, 1484, 1859
HaeIII GGCC 7 cut(s) 267, 514, 963, 1042, 1451, 1939, 1947
HapII CCGG 3 cut(s) 173, 749, 972
HgaI GACGC 6 cut(s) 457, 741, 842, 958, 1070, 1727
HhaI GCGC 3 cut(s) 845, 905, 1457
Hin1I GRCGYC 1 cut(s) 752
Hin1II CATG 8 cut(s) 69, 155, 299, 1270, 1330, 1544, 1649, 1688
Hin6I GCGC 3 cut(s) 843, 903, 1455
HinP1I GCGC 3 cut(s) 843, 903, 1455
HincII GTYRAC 3 cut(s) 898, 1029, 1561
HindII GTYRAC 3 cut(s) 898, 1029, 1561
HindIII AAGCTT 2 cut(s) 182, 1926
HinfI GANTC 7 cut(s) 169, 911, 1220, 1283, 1323, 1510, 1598
HpaII CCGG 3 cut(s) 173, 749, 972
HphI GGTGA 5 cut(s) 245, 1076, 1556, 1604, 1702
Hpy166II GTNNAC 8 cut(s) 898, 1029, 1165, 1561, 1597, 1710, 1767, 1769
Hpy188I TCNGA 4 cut(s) 47, 1105, 1288, 1855
Hpy188III TCNNGA 8 cut(s) 152, 337, 393, 506, 563, 1267, 1838, 1908
Hpy8I GTNNAC 8 cut(s) 898, 1029, 1165, 1561, 1597, 1710, 1767, 1769
Hpy99I CGWCG 6 cut(s) 757, 766, 788, 858, 899, 962
HpyAV CCTTC 5 cut(s) 124, 346, 602, 716, 982
HpyCH4IV ACGT 7 cut(s) 90, 397, 730, 770, 862, 1025, 1148
HpyF10VI GCNNNNNNNGC 8 cut(s) 76, 497, 749, 774, 1368, 1802, 1811, 1823
HpyF3I CTNAG 9 cut(s) 44, 144, 165, 778, 828, 1285, 1347, 1620, 1774
HpySE526I ACGT 7 cut(s) 90, 397, 730, 770, 862, 1025, 1148
Hsp92I GRCGYC 1 cut(s) 752
Hsp92II CATG 8 cut(s) 69, 155, 299, 1270, 1330, 1544, 1649, 1688
HspAI GCGC 3 cut(s) 843, 903, 1455
Kzo9I GATC 4 cut(s) 598, 1233, 1307, 1546
LmnI GCTCC 7 cut(s) 565, 659, 820, 896, 1098, 1465, 1840
Lsp1109I GCAGC 1 cut(s) 500
LweI GCATC 4 cut(s) 373, 460, 693, 1426
MabI ACCWGGT 2 cut(s) 1582, 1915
MaeI CTAG 3 cut(s) 920, 1092, 1212
MaeII ACGT 7 cut(s) 90, 397, 730, 770, 862, 1025, 1148
MaeIII GTNAC 6 cut(s) 52, 976, 1082, 1486, 1610, 1846
MalI GATC 4 cut(s) 600, 1235, 1309, 1548
MbiI CCGCTC 1 cut(s) 777
MboI GATC 4 cut(s) 598, 1233, 1307, 1546
MboII GAAGA 4 cut(s) 352, 1285, 1288, 1324
MfeI CAATTG 3 cut(s) 483, 1126, 1194
MflI RGATCY 2 cut(s) 598, 1233
MlyI GAGTC 5 cut(s) 905, 1229, 1277, 1519, 1592
MseI TTAA 6 cut(s) 137, 239, 287, 303, 1062, 1277
MslI CAYNNNNRTG 1 cut(s) 1683
MspA1I CMGCKG 1 cut(s) 491
MspI CCGG 3 cut(s) 173, 749, 972
MspR9I CCNGG 5 cut(s) 174, 269, 462, 1584, 1917
MunI CAATTG 3 cut(s) 483, 1126, 1194
Mva1269I GAATGC 2 cut(s) 1060, 1188
MvaI CCWGG 4 cut(s) 269, 462, 1584, 1917
MvnI CGCG 3 cut(s) 843, 903, 1817
MwoI GCNNNNNNNGC 8 cut(s) 76, 497, 749, 774, 1368, 1802, 1811, 1823
NciI CCSGG 1 cut(s) 174
NcoI CCATGG 1 cut(s) 1540
NdeI CATATG 1 cut(s) 1756
NdeII GATC 4 cut(s) 598, 1233, 1307, 1546
NheI GCTAGC 1 cut(s) 1211
NlaIII CATG 8 cut(s) 69, 155, 299, 1270, 1330, 1544, 1649, 1688
NlaIV GGNNCC 4 cut(s) 600, 892, 1467, 1842
NmeAIII GCCGAG 2 cut(s) 867, 1024
NmuCI GTSAC 5 cut(s) 52, 1082, 1486, 1610, 1846
NspI RCATGY 1 cut(s) 299
PaeR7I CTCGAG 1 cut(s) 1908
PagI TCATGA 2 cut(s) 151, 1266
PceI AGGCCT 4 cut(s) 1042, 1451, 1939, 1947
PciI ACATGT 1 cut(s) 295
PcsI WCGNNNNNNNCGW 4 cut(s) 761, 792, 894, 1906
PctI GAATGC 2 cut(s) 1060, 1188
PfeI GAWTC 2 cut(s) 169, 1323
PflFI GACNNNGTC 1 cut(s) 616
PkrI GCNGC 4 cut(s) 490, 847, 1670, 1816
PleI GAGTC 5 cut(s) 905, 1228, 1277, 1518, 1592
PpsI GAGTC 5 cut(s) 905, 1228, 1277, 1518, 1592
Ppu21I YACGTR 2 cut(s) 91, 731
PscI ACATGT 1 cut(s) 295
Psp124BI GAGCTC 3 cut(s) 570, 1103, 1828
Psp1406I AACGTT 1 cut(s) 1148
Psp6I CCWGG 4 cut(s) 267, 460, 1582, 1915
PspFI CCCAGC 2 cut(s) 849, 1672
PspGI CCWGG 4 cut(s) 267, 460, 1582, 1915
PspN4I GGNNCC 4 cut(s) 600, 892, 1467, 1842
PstI CTGCAG 1 cut(s) 1728
PstNI CAGNNNCTG 1 cut(s) 85
PsuI RGATCY 2 cut(s) 598, 1233
PsyI GACNNNGTC 1 cut(s) 616
PvuII CAGCTG 1 cut(s) 491
RseI CAYNNNNRTG 1 cut(s) 1683
SacI GAGCTC 3 cut(s) 570, 1103, 1828
SalI GTCGAC 1 cut(s) 896
SaqAI TTAA 6 cut(s) 137, 239, 287, 303, 1062, 1277
SatI GCNGC 4 cut(s) 489, 846, 1669, 1815
Sau3AI GATC 4 cut(s) 598, 1233, 1307, 1546
ScaI AGTACT 1 cut(s) 375
SchI GAGTC 5 cut(s) 905, 1229, 1277, 1519, 1592
ScrFI CCNGG 5 cut(s) 174, 269, 462, 1584, 1917
SexAI ACCWGGT 2 cut(s) 1582, 1915
SfaNI GCATC 4 cut(s) 373, 460, 693, 1426
SfcI CTRYAG 3 cut(s) 690, 1639, 1724
Sfr274I CTCGAG 1 cut(s) 1908
SlaI CTCGAG 1 cut(s) 1908
SmiMI CAYNNNNRTG 1 cut(s) 1683
SmlI CTYRAG 3 cut(s) 504, 549, 1908
SmoI CTYRAG 3 cut(s) 504, 549, 1908
SnaBI TACGTA 1 cut(s) 731
SseBI AGGCCT 4 cut(s) 1042, 1451, 1939, 1947
SsiI CCGC 6 cut(s) 775, 846, 901, 1669, 1713, 1815
SspMI CTAG 3 cut(s) 920, 1092, 1212
SstI GAGCTC 3 cut(s) 570, 1103, 1828
StuI AGGCCT 4 cut(s) 1042, 1451, 1939, 1947
StyD4I CCNGG 5 cut(s) 172, 267, 460, 1582, 1915
StyI CCWWGG 2 cut(s) 405, 1540
TaiI ACGT 7 cut(s) 93, 400, 733, 773, 865, 1028, 1151
TaqI TCGA 6 cut(s) 112, 764, 795, 897, 957, 1909
TatI WGTACW 4 cut(s) 3, 373, 1162, 1766
TauI GCSGC 3 cut(s) 848, 1671, 1817
TfiI GAWTC 2 cut(s) 169, 1323
Tru1I TTAA 6 cut(s) 137, 239, 287, 303, 1062, 1277
Tru9I TTAA 6 cut(s) 137, 239, 287, 303, 1062, 1277
TscAI CASTG 8 cut(s) 112, 284, 596, 1174, 1180, 1467, 1728, 1864
TseFI GTSAC 5 cut(s) 52, 1082, 1486, 1610, 1846
TseI GCWGC 1 cut(s) 488
Tsp45I GTSAC 5 cut(s) 52, 1082, 1486, 1610, 1846
TspDTI ATGAA 5 cut(s) 50, 323, 639, 865, 1713
TspGWI ACGGA 3 cut(s) 379, 883, 1915
TspRI CASTG 8 cut(s) 112, 284, 596, 1174, 1180, 1467, 1728, 1864
Tth111I GACNNNGTC 1 cut(s) 616
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 3 cut(s) 469, 966, 1653
XceI RCATGY 1 cut(s) 299
XhoI CTCGAG 1 cut(s) 1908
XmiI GTMKAC 1 cut(s) 897
XspI CTAG 3 cut(s) 920, 1092, 1212
ZrmI AGTACT 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.