RLG00000001249

Polysaccharide lyase family 4, domain III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014889
Physical Location & Seq
Reverse (-)
12815952 .. 12820859
4908 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001249

Sequence Viewer

Length: 1917 bp
ATGTCAGGTGCAGGAGTGCGGTTACATAATCTGGGTCCCCATGTGGTTATGGATAATGGCATAGTCCAAGTCACCTTATCAAACCCAGATGGTATTGTCACCGGAATACGATATAATGGGATTGACAATTTGCTTGAACCTCTCAATCCTGAACATAATAGAGGTTATTGGGACCTTGTCTGGAGTGGACCGGGAGTTAAGGGAACATTTGATGTGATAAAAGGTACTACTTTTAGGGTTATTGTGGAGAATGAGGAACAGGTCGAGCTCTCGTTTACAAGAACGTGGAATCCCTCTCTTGAGGGGAAATATGTTCCCTTAAACATAGACAAAAGGTTTGTAATGCTTCGTGGTTCCTCGGGCTTCTACTCCTATGCAATTTATGAGCACCTGAAGGATTGGCCTGATTTTGAGATTGGTGAAACCAGAATAACTTTCAAACTGAGAAAAGACAAGTTCCAATACATGGCTATATCAGACAACAGGCAAAGATACATGCCCCTACCTGATGACCGGATGCCAGGAAGATGCCAGCCCCTGGCTTACCCAGAAGCTGTCCTACTTCTGAATCCCAAGATGCAAGAGCTTGCCGGAGAGGTAGATGACAAGTACCAGTACTCATGTGTGAACAAAGACAATCAAGTTCATGGTTGGATATGCACCAACCCACCAGTCGGCTTTTGGCAGATCACACCAAGTGATGAATTCCGGTCTGGTGGACCTCTGAAACAGAATCTAACCTCACATGTTGGCCCCACCACTCTTGCAATGTTTCATAGTTCTCATTATGCGGGACAAAATCTGGTGCCAAAATTTAGAGGTGGTGAACCATGGAAGAAGGTTTTTGGCCCTGTTTTTATCTATCTCAATTCTGCGGGAATTAGAGATGATCCATTCTTTTTATGGGAGGATGCTAAAATTAAGATGATGGCAGAAGTCCAAAGCTGGCCGTATAGTTTTCCAGCTTCTGAGGATTTCCAAAAGTCGGATCAACGGGGTAATGTGAGTGGTAGACTACTTGTCTTGGACAGGTATGTTAGTGAAGAATATATACCAGCAAATGATGCATATGTTGGTTTGGCTCCACCAGGAGATGTAGGATCATGGCAACGAGAATGCAAGGACTATCAATTCTGGTCGAGGGCAGATAACAGAGGTTACTTTTGCATTAACAACATACGTACTGGCACGTACAATCTTTATGCCTGGGTCCCTGGTTTTATTGGAGATTACCGGTATGAAGCAGTCATTAACATAACCTCAGGTTCTTTTACTGAAATGGGTGATCTTGTGTATGAACCTCCAAGAGATGGGCCTACATTGTGGGAAATAGGCATTCCTGATCGTTCCGCTGCAGAGTTTTATGTTCCTGATCCCGATCCGAAGCATATTAATAAGCTTTTTGTTAATCATCCTGACAGGTTCAGGCAGTATGGTCTGTGGAACAGATATACCGAATTATATCCTCATGAAGATTTGGTTTATACTGTTGGTGTTAGTGAGTATAGTAAAGATTGGTTCTTTGCTCAGATTCCCAGGCAAAAGGACAACACACATGTAGGAACAACATGGCAAATTAAGTTCAAACTTAACAATGTGGATCGAAACGGTACCTATAAACTGCGAGTGGCAATTGCATCTGCAACTCTAGCTGAAATGCAGGTTCGTATCAATGATCCAAATGCAAGACGACCTCTATTTACGACTGGACTGATAGGGAGAGATAACTCAGTTGCGAGACATGGAGTTCATGGGATCTACTGGCTGTTCAACATCAATGTACCGGGTGCTCAGCTTGTTGAGGGTGATAATACTATCTTCCTGACACAACCAAGATGCACCAGTCCTTTCCAAGGGCTTATGTATGATTACTTACGACTTGAAGGCCCCCCATCTTGTGGTTCGAGTTCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

639

Amino Acids

72.88

Weight (kDa)

6.01

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 13 - 200 1.5e-92 Rhamnogalacturonate lyase family
fn3_3 PF14686 354 - 425 3.9e-26 Polysaccharide lyase family 4, domain II
CBM-like PF14683 440 - 627 1.9e-52 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014889)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09910 AT1G09910 AT1G09910
fragaria_vesca FvH4_5g34110 FvH4_5g34110
malus_domestica MD08G1205900.v1.1
prunus_persica Prupe.1G538400_v2.0.a1 Prupe.1G538400_v2.0.a1
pyrus_communis pycom08g17810 pycom15g34900
rosa_chinensis RchiOBHm_Chr7g0233611
rosa_laevigata RLG00000001249
rosa_rugosa Rorug07G0281500 Rorug07G0281600
rosa_samantha Rh7AG438100 Rh7BG410800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1651
Acc65I GGTACC 1 cut(s) 1610
AccB1I GGYRCC 2 cut(s) 805, 1610
AccB7I CCANNNNNTGG 4 cut(s) 466, 538, 1310, 1898
AccI GTMKAC 1 cut(s) 1012
AciI CCGC 4 cut(s) 19, 791, 875, 1350
AclWI GGATC 8 cut(s) 884, 996, 1108, 1367, 1373, 1608, 1670, 1763
AcoI YGGCCR 1 cut(s) 947
AcsI RAATTY 2 cut(s) 704, 812
AcuI CTGAAG 1 cut(s) 413
AdeI CACNNNGTG 1 cut(s) 698
AfaI GTAC 7 cut(s) 226, 611, 617, 1183, 1193, 1612, 1782
AfiI CCNNNNNNNGG 7 cut(s) 466, 538, 674, 985, 1310, 1853, 1898
AflIII ACRYGT 2 cut(s) 745, 1555
AgeI ACCGGT 1 cut(s) 1233
AgsI TTSAA 5 cut(s) 137, 439, 1585, 1771, 1883
AhdI GACNNNNNGTC 1 cut(s) 1019
AjnI CCWGG 6 cut(s) 520, 537, 1087, 1205, 1213, 1535
AloI GAACNNNNNNTCC 2 cut(s) 274, 306
AluBI AGCT 8 cut(s) 268, 554, 586, 945, 965, 1399, 1652, 1795
AluI AGCT 8 cut(s) 268, 554, 586, 945, 965, 1399, 1652, 1795
Alw21I GWGCWC 3 cut(s) 270, 390, 1792
Alw26I GTCTC 1 cut(s) 1732
AlwI GGATC 8 cut(s) 884, 996, 1108, 1367, 1373, 1608, 1670, 1763
AlwNI CAGNNNCTG 3 cut(s) 538, 554, 968
Ama87I CYCGRG 1 cut(s) 358
AoxI GGCC 6 cut(s) 401, 751, 847, 947, 1313, 1885
ApeKI GCWGC 1 cut(s) 1352
ApoI RAATTY 2 cut(s) 704, 812
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
AseI ATTAAT 1 cut(s) 1392
AsiGI ACCGGT 1 cut(s) 1233
Asp718I GGTACC 1 cut(s) 1610
AspS9I GGNCC 9 cut(s) 35, 172, 188, 719, 752, 848, 1210, 1313, 1886
AsuC2I CCSGG 2 cut(s) 192, 1785
AsuHPI GGTGA 6 cut(s) 64, 91, 431, 836, 1295, 1817
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 5 cut(s) 35, 172, 188, 719, 1210
AxyI CCTNAGG 1 cut(s) 1261
BanI GGYRCC 2 cut(s) 805, 1610
BanII GRGCYC 1 cut(s) 270
BarI GAAGNNNNNNTAC 4 cut(s) 543, 575, 1035, 1067
Bbv12I GWGCWC 3 cut(s) 270, 390, 1792
BbvI GCAGC 1 cut(s) 1339
BccI CCATC 4 cut(s) 83, 922, 1304, 1900
BceAI ACGGC 1 cut(s) 934
BciT130I CCWGG 6 cut(s) 522, 539, 1089, 1207, 1215, 1537
BcnI CCSGG 2 cut(s) 192, 1785
BcoDI GTCTC 1 cut(s) 1732
BfaI CTAG 1 cut(s) 1649
BfmI CTRYAG 1 cut(s) 1353
BfuAI ACCTGC 1 cut(s) 1651
BisI GCNGC 1 cut(s) 1353
BlpI GCTNAGC 1 cut(s) 1791
BlsI GCNGC 1 cut(s) 1354
BmcAI AGTACT 1 cut(s) 617
Bme1390I CCNGG 8 cut(s) 192, 522, 539, 1089, 1207, 1215, 1537, 1785
Bme18I GGWCC 5 cut(s) 35, 172, 188, 719, 1210
BmeRI GACNNNNNGTC 1 cut(s) 1019
BmeT110I CYCGRG 1 cut(s) 358
BmgT120I GGNCC 9 cut(s) 35, 172, 188, 719, 752, 848, 1210, 1313, 1886
BmrFI CCNGG 8 cut(s) 192, 522, 539, 1089, 1207, 1215, 1537, 1785
BmsI GCATC 7 cut(s) 507, 518, 567, 901, 1054, 1646, 1826
BpmI CTGGAG 1 cut(s) 202
Bpu1102I GCTNAGC 1 cut(s) 1791
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 2 cut(s) 192, 1785
BsaAI YACGTR 2 cut(s) 1181, 1191
BsaBI GATNNNNATC 1 cut(s) 1377
BsaJI CCNNGG 7 cut(s) 357, 537, 830, 1206, 1213, 1535, 1852
BsaWI WCCGGW 4 cut(s) 101, 513, 708, 1233
Bsc4I CCNNNNNNNGG 7 cut(s) 466, 538, 674, 985, 1310, 1853, 1898
Bse118I RCCGGY 1 cut(s) 1233
Bse1I ACTGG 6 cut(s) 613, 671, 1189, 1711, 1766, 1842
Bse21I CCTNAGG 1 cut(s) 1261
Bse3DI GCAATG 1 cut(s) 774
Bse8I GATNNNNATC 1 cut(s) 1377
BseBI CCWGG 6 cut(s) 522, 539, 1089, 1207, 1215, 1537
BseDI CCNNGG 7 cut(s) 357, 537, 830, 1206, 1213, 1535, 1852
BseGI GGATG 3 cut(s) 522, 916, 1411
BseJI GATNNNNATC 1 cut(s) 1377
BseLI CCNNNNNNNGG 7 cut(s) 466, 538, 674, 985, 1310, 1853, 1898
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 6 cut(s) 434, 960, 1275, 1541, 1743, 1805
BseNI ACTGG 6 cut(s) 613, 671, 1189, 1711, 1766, 1842
BseXI GCAGC 1 cut(s) 1339
BsgI GTGCAG 1 cut(s) 30
BshFI GGCC 6 cut(s) 403, 753, 849, 949, 1315, 1887
BshNI GGYRCC 2 cut(s) 805, 1610
BshTI ACCGGT 1 cut(s) 1233
BsiHKAI GWGCWC 3 cut(s) 270, 390, 1792
BsiHKCI CYCGRG 1 cut(s) 358
BsiSI CCGG 7 cut(s) 102, 191, 514, 591, 709, 1234, 1784
BslFI GGGAC 4 cut(s) 21, 185, 807, 1196
BslI CCNNNNNNNGG 7 cut(s) 466, 538, 674, 985, 1310, 1853, 1898
BsmAI GTCTC 1 cut(s) 1732
BsmFI GGGAC 4 cut(s) 21, 185, 807, 1196
BsmI GAATGC 2 cut(s) 1121, 1335
BsnI GGCC 6 cut(s) 403, 753, 849, 949, 1315, 1887
BsoBI CYCGRG 1 cut(s) 358
Bsp1286I GDGCHC 3 cut(s) 270, 390, 1792
Bsp1720I GCTNAGC 1 cut(s) 1791
Bsp19I CCATGG 1 cut(s) 830
BspACI CCGC 4 cut(s) 19, 791, 875, 1350
BspANI GGCC 6 cut(s) 403, 753, 849, 949, 1315, 1887
BspCNI CTCAG 6 cut(s) 435, 961, 1274, 1540, 1742, 1804
BspHI TCATGA 1 cut(s) 1468
BspMAI CTGCAG 1 cut(s) 1357
BspMI ACCTGC 1 cut(s) 1651
BspPI GGATC 8 cut(s) 884, 996, 1108, 1367, 1373, 1608, 1670, 1763
BspT107I GGYRCC 2 cut(s) 805, 1610
BsrDI GCAATG 1 cut(s) 774
BsrFI RCCGGY 1 cut(s) 1233
BsrI ACTGG 6 cut(s) 613, 671, 1189, 1711, 1766, 1842
BssAI RCCGGY 1 cut(s) 1233
BssECI CCNNGG 7 cut(s) 357, 537, 830, 1206, 1213, 1535, 1852
BssT1I CCWWGG 2 cut(s) 830, 1852
Bst2UI CCWGG 6 cut(s) 522, 539, 1089, 1207, 1215, 1537
Bst4CI ACNGT 2 cut(s) 1489, 1610
BstAPI GCANNNNNTGC 1 cut(s) 1064
BstBAI YACGTR 2 cut(s) 1181, 1191
BstC8I GCNNGC 3 cut(s) 533, 588, 947
BstDEI CTNAG 6 cut(s) 443, 969, 1261, 1527, 1729, 1791
BstDSI CCRYGG 1 cut(s) 830
BstENI CCTNNNNNAGG 1 cut(s) 1851
BstF5I GGATG 3 cut(s) 522, 916, 1411
BstMAI GTCTC 1 cut(s) 1732
BstMWI GCNNNNNNNGC 2 cut(s) 1064, 1649
BstNI CCWGG 6 cut(s) 522, 539, 1089, 1207, 1215, 1537
BstNSI RCATGY 3 cut(s) 499, 749, 1559
BstSCI CCNGG 8 cut(s) 190, 520, 537, 1087, 1205, 1213, 1535, 1783
BstSFI CTRYAG 1 cut(s) 1353
BstSNI TACGTA 1 cut(s) 1181
BstV1I GCAGC 1 cut(s) 1339
BstX2I RGATCY 1 cut(s) 1755
BstYI RGATCY 1 cut(s) 1755
Bsu36I CCTNAGG 1 cut(s) 1261
BsuRI GGCC 6 cut(s) 403, 753, 849, 949, 1315, 1887
BtgI CCRYGG 1 cut(s) 830
BtsCI GGATG 3 cut(s) 522, 916, 1411
BveI ACCTGC 1 cut(s) 1651
Cac8I GCNNGC 3 cut(s) 533, 588, 947
CaiI CAGNNNCTG 3 cut(s) 538, 554, 968
CciI TCATGA 1 cut(s) 1468
Cfr10I RCCGGY 1 cut(s) 1233
Cfr13I GGNCC 9 cut(s) 35, 172, 188, 719, 752, 848, 1210, 1313, 1886
Csp6I GTAC 7 cut(s) 225, 610, 616, 1182, 1192, 1611, 1781
CspAI ACCGGT 1 cut(s) 1233
CspCI CAANNNNNGTGG 2 cut(s) 745, 780
CviQI GTAC 7 cut(s) 225, 610, 616, 1182, 1192, 1611, 1781
DdeI CTNAG 6 cut(s) 443, 969, 1261, 1527, 1729, 1791
DraIII CACNNNGTG 1 cut(s) 698
DriI GACNNNNNGTC 1 cut(s) 1019
EaeI YGGCCR 1 cut(s) 947
Eam1105I GACNNNNNGTC 1 cut(s) 1019
Ecl136II GAGCTC 1 cut(s) 268
Eco105I TACGTA 1 cut(s) 1181
Eco130I CCWWGG 2 cut(s) 830, 1852
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 5 cut(s) 35, 172, 188, 719, 1210
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 413
Eco81I CCTNAGG 1 cut(s) 1261
Eco88I CYCGRG 1 cut(s) 358
EcoICRI GAGCTC 1 cut(s) 268
EcoNI CCTNNNNNAGG 1 cut(s) 1851
EcoO109I RGGNCCY 4 cut(s) 35, 172, 1210, 1886
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 6 cut(s) 520, 537, 1087, 1205, 1213, 1535
EcoT14I CCWWGG 2 cut(s) 830, 1852
EcoT22I ATGCAT 1 cut(s) 1069
EcoT38I GRGCYC 1 cut(s) 270
ErhI CCWWGG 2 cut(s) 830, 1852
FaqI GGGAC 4 cut(s) 21, 185, 807, 1196
FauI CCCGC 2 cut(s) 784, 868
FauNDI CATATG 1 cut(s) 1069
FblI GTMKAC 1 cut(s) 1012
Fnu4HI GCNGC 1 cut(s) 1353
FokI GGATG 3 cut(s) 529, 923, 1398
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 1 cut(s) 1353
FspBI CTAG 1 cut(s) 1649
GluI GCNGC 1 cut(s) 1353
GsuI CTGGAG 1 cut(s) 202
HaeIII GGCC 6 cut(s) 403, 753, 849, 949, 1315, 1887
HapII CCGG 7 cut(s) 102, 191, 514, 591, 709, 1234, 1784
HindIII AAGCTT 1 cut(s) 1397
HinfI GANTC 4 cut(s) 289, 568, 733, 1531
HpaII CCGG 7 cut(s) 102, 191, 514, 591, 709, 1234, 1784
HphI GGTGA 6 cut(s) 64, 91, 431, 836, 1295, 1817
Hpy166II GTNNAC 6 cut(s) 188, 276, 628, 719, 827, 1013
Hpy188I TCNGA 7 cut(s) 478, 567, 726, 970, 988, 1383, 1530
Hpy188III TCNNGA 9 cut(s) 149, 181, 299, 1340, 1370, 1376, 1415, 1469, 1822
Hpy8I GTNNAC 6 cut(s) 188, 276, 628, 719, 827, 1013
HpyAV CCTTC 3 cut(s) 388, 832, 1877
HpyCH4III ACNGT 2 cut(s) 1489, 1610
HpyCH4IV ACGT 3 cut(s) 284, 1180, 1190
HpyF10VI GCNNNNNNNGC 2 cut(s) 1064, 1649
HpyF3I CTNAG 6 cut(s) 443, 969, 1261, 1527, 1729, 1791
HpySE526I ACGT 3 cut(s) 284, 1180, 1190
KflI GGGWCCC 2 cut(s) 35, 1210
KpnI GGTACC 1 cut(s) 1614
LmnI GCTCC 1 cut(s) 1087
Lsp1109I GCAGC 1 cut(s) 1339
LweI GCATC 7 cut(s) 507, 518, 567, 901, 1054, 1646, 1826
MaeI CTAG 1 cut(s) 1649
MaeII ACGT 3 cut(s) 284, 1180, 1190
MaeIII GTNAC 4 cut(s) 21, 70, 97, 1157
MboII GAAGA 5 cut(s) 537, 847, 1055, 1484, 1810
MfeI CAATTG 1 cut(s) 1632
MflI RGATCY 1 cut(s) 1755
MhlI GDGCHC 3 cut(s) 270, 390, 1792
MmeI TCCRAC 2 cut(s) 632, 966
Mph1103I ATGCAT 1 cut(s) 1069
MspA1I CMGCKG 1 cut(s) 1352
MspI CCGG 7 cut(s) 102, 191, 514, 591, 709, 1234, 1784
MspR9I CCNGG 8 cut(s) 192, 522, 539, 1089, 1207, 1215, 1537, 1785
MunI CAATTG 1 cut(s) 1632
Mva1269I GAATGC 2 cut(s) 1121, 1335
MvaI CCWGG 6 cut(s) 522, 539, 1089, 1207, 1215, 1537
MwoI GCNNNNNNNGC 2 cut(s) 1064, 1649
NciI CCSGG 2 cut(s) 192, 1785
NcoI CCATGG 1 cut(s) 830
NdeI CATATG 1 cut(s) 1069
NmuCI GTSAC 2 cut(s) 70, 97
NsiI ATGCAT 1 cut(s) 1069
NspI RCATGY 3 cut(s) 499, 749, 1559
PagI TCATGA 1 cut(s) 1468
PciI ACATGT 2 cut(s) 745, 1555
PctI GAATGC 2 cut(s) 1121, 1335
PfeI GAWTC 4 cut(s) 289, 568, 733, 1531
PflFI GACNNNGTC 1 cut(s) 176
PflMI CCANNNNNTGG 4 cut(s) 466, 538, 1310, 1898
PinAI ACCGGT 1 cut(s) 1233
PkrI GCNGC 1 cut(s) 1354
Ppu21I YACGTR 2 cut(s) 1181, 1191
PpuMI RGGWCCY 3 cut(s) 35, 172, 1210
PscI ACATGT 2 cut(s) 745, 1555
PshBI ATTAAT 1 cut(s) 1392
Psp124BI GAGCTC 1 cut(s) 270
Psp5II RGGWCCY 3 cut(s) 35, 172, 1210
Psp6I CCWGG 6 cut(s) 520, 537, 1087, 1205, 1213, 1535
PspGI CCWGG 6 cut(s) 520, 537, 1087, 1205, 1213, 1535
PspPI GGNCC 9 cut(s) 35, 172, 188, 719, 752, 848, 1210, 1313, 1886
PspPPI RGGWCCY 3 cut(s) 35, 172, 1210
PstI CTGCAG 1 cut(s) 1357
PstNI CAGNNNCTG 3 cut(s) 538, 554, 968
PsuI RGATCY 1 cut(s) 1755
PsyI GACNNNGTC 1 cut(s) 176
RsaI GTAC 7 cut(s) 226, 611, 617, 1183, 1193, 1612, 1782
RsaNI GTAC 7 cut(s) 225, 610, 616, 1182, 1192, 1611, 1781
SacI GAGCTC 1 cut(s) 270
SatI GCNGC 1 cut(s) 1353
Sau96I GGNCC 9 cut(s) 35, 172, 188, 719, 752, 848, 1210, 1313, 1886
ScaI AGTACT 1 cut(s) 617
ScrFI CCNGG 8 cut(s) 192, 522, 539, 1089, 1207, 1215, 1537, 1785
SduI GDGCHC 3 cut(s) 270, 390, 1792
SfaNI GCATC 7 cut(s) 507, 518, 567, 901, 1054, 1646, 1826
SfcI CTRYAG 1 cut(s) 1353
SinI GGWCC 5 cut(s) 35, 172, 188, 719, 1210
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
SnaBI TACGTA 1 cut(s) 1181
SsiI CCGC 4 cut(s) 19, 791, 875, 1350
SspMI CTAG 1 cut(s) 1649
SstI GAGCTC 1 cut(s) 270
StyD4I CCNGG 8 cut(s) 190, 520, 537, 1087, 1205, 1213, 1535, 1783
StyI CCWWGG 2 cut(s) 830, 1852
TaaI ACNGT 2 cut(s) 1489, 1610
TaiI ACGT 3 cut(s) 287, 1183, 1193
TaqI TCGA 4 cut(s) 264, 1139, 1603, 1904
TatI WGTACW 1 cut(s) 615
TfiI GAWTC 4 cut(s) 289, 568, 733, 1531
TseFI GTSAC 2 cut(s) 70, 97
TseI GCWGC 1 cut(s) 1352
Tsp45I GTSAC 2 cut(s) 70, 97
TspDTI ATGAA 8 cut(s) 635, 717, 764, 1254, 1311, 1485, 1739, 1899
Tth111I GACNNNGTC 1 cut(s) 176
Van91I CCANNNNNTGG 4 cut(s) 466, 538, 1310, 1898
VpaK11BI GGWCC 5 cut(s) 35, 172, 188, 719, 1210
VspI ATTAAT 1 cut(s) 1392
XagI CCTNNNNNAGG 1 cut(s) 1851
XapI RAATTY 2 cut(s) 704, 812
XceI RCATGY 3 cut(s) 499, 749, 1559
XcmI CCANNNNNNNNNTGG 2 cut(s) 678, 900
XmiI GTMKAC 1 cut(s) 1012
XspI CTAG 1 cut(s) 1649
ZrmI AGTACT 1 cut(s) 617
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.