Rorug07G0281600

Polysaccharide lyase family 4, domain III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0014889
Physical Location & Seq
Forward (+)
27334815 .. 27348509
13695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0281600.1

Sequence Viewer

Length: 402 bp
ATGCCGCCGGCGCTCTCGCTGCTGCGTTCCGGTCCGGGAGAGGATTTGGTTCTGGGATGGCAGCGTTTGTCGTTGGCGGAAGCGAGTTCCATGGATGGAGTTAGGAGGGTCGAGCTCGGGTCTGGCAGATCGTTTTCGATCTGTCTTCTCCGAATCGTGGCCGATGGCAGACGAGGGGTGGCGCTTGATTGCGGCTTGCCGGAAGCGATATCTGGTGTCTATGCTGCATCGGAGATCTGTGCGGCTACTGGATGCTCGATCCCATGCCAGAGGCTCTATGATGTACGACTTCTTTATGAGAAATTGGCTGCTGTTGTGACGAAAAAAAAGGGGTCAGAGGAGCCACTTGGACTTTTCTTCACACTCTTGCTGCTCAGGAGGCTTGCAACAACACTAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

133

Amino Acids

14.27

Weight (kDa)

8.34

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014889)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09910 AT1G09910 AT1G09910
fragaria_vesca FvH4_5g34110 FvH4_5g34110
malus_domestica MD08G1205900.v1.1
prunus_persica Prupe.1G538400_v2.0.a1 Prupe.1G538400_v2.0.a1
pyrus_communis pycom08g17810 pycom15g34900
rosa_chinensis RchiOBHm_Chr7g0233611
rosa_laevigata RLG00000001249
rosa_rugosa Rorug07G0281500 Rorug07G0281600
rosa_samantha Rh7AG438100 Rh7BG410800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 5, 77, 192, 242
AclWI GGATC 1 cut(s) 253
AcoI YGGCCR 1 cut(s) 159
AfaI GTAC 1 cut(s) 285
AfiI CCNNNNNNNGG 1 cut(s) 157
AluBI AGCT 1 cut(s) 115
AluI AGCT 1 cut(s) 115
Alw21I GWGCWC 1 cut(s) 117
AlwI GGATC 1 cut(s) 253
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 1 cut(s) 159
ApeKI GCWGC 6 cut(s) 19, 22, 61, 224, 308, 370
AspLEI GCGC 2 cut(s) 13, 184
AspS9I GGNCC 1 cut(s) 32
AsuC2I CCSGG 1 cut(s) 36
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 1 cut(s) 32
BanII GRGCYC 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 137
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 6 cut(s) 6, 9, 73, 211, 295, 357
BccI CCATC 3 cut(s) 51, 89, 158
BcnI CCSGG 1 cut(s) 36
BfaI CTAG 1 cut(s) 395
BfoI RGCGCY 2 cut(s) 14, 185
BglII AGATCT 1 cut(s) 234
BisI GCNGC 9 cut(s) 5, 20, 23, 62, 193, 225, 243, 309, 371
BlsI GCNGC 9 cut(s) 6, 21, 24, 63, 194, 226, 244, 310, 372
Bme1390I CCNGG 1 cut(s) 36
Bme18I GGWCC 1 cut(s) 32
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 32
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 1 cut(s) 36
BmsI GCATC 2 cut(s) 236, 242
BpiI GAAGAC 1 cut(s) 137
Bpu10I CCTNAGC 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 90
BsaWI WCCGGW 1 cut(s) 29
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 1 cut(s) 253
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 3 cut(s) 62, 100, 257
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 388
BseNI ACTGG 1 cut(s) 253
BseRI GAGGAG 1 cut(s) 353
BseXI GCAGC 6 cut(s) 6, 9, 73, 211, 295, 357
BshFI GGCC 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 4 cut(s) 8, 30, 35, 200
BslI CCNNNNNNNGG 1 cut(s) 157
BsnI GGCC 1 cut(s) 161
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 4 cut(s) 128, 138, 234, 258
Bsp19I CCATGG 1 cut(s) 90
BspACI CCGC 4 cut(s) 5, 77, 192, 242
BspANI GGCC 1 cut(s) 161
BspCNI CTCAG 1 cut(s) 387
BspLI GGNNCC 1 cut(s) 342
BspPI GGATC 1 cut(s) 253
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 1 cut(s) 253
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 4 cut(s) 128, 138, 234, 258
BssT1I CCWWGG 1 cut(s) 90
BstC8I GCNNGC 3 cut(s) 9, 197, 384
BstDEI CTNAG 1 cut(s) 374
BstDSI CCRYGG 1 cut(s) 90
BstF5I GGATG 3 cut(s) 62, 100, 257
BstH2I RGCGCY 2 cut(s) 14, 185
BstHHI GCGC 2 cut(s) 13, 184
BstKTI GATC 4 cut(s) 131, 141, 237, 261
BstMBI GATC 4 cut(s) 128, 138, 234, 258
BstMWI GCNNNNNNNGC 3 cut(s) 10, 19, 379
BstSCI CCNGG 1 cut(s) 34
BstV1I GCAGC 6 cut(s) 6, 9, 73, 211, 295, 357
BstV2I GAAGAC 1 cut(s) 137
BstX2I RGATCY 1 cut(s) 234
BstYI RGATCY 1 cut(s) 234
BsuRI GGCC 1 cut(s) 161
BtgI CCRYGG 1 cut(s) 90
BtsCI GGATG 3 cut(s) 62, 100, 257
Cac8I GCNNGC 3 cut(s) 9, 197, 384
CfoI GCGC 2 cut(s) 13, 184
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 32
CpoI CGGWCCG 1 cut(s) 32
Csp6I GTAC 1 cut(s) 284
CspI CGGWCCG 1 cut(s) 32
CviAII CATG 2 cut(s) 91, 264
CviJI RGCY 8 cut(s) 115, 161, 195, 245, 274, 308, 343, 382
CviKI_1 RGCY 8 cut(s) 115, 161, 195, 245, 274, 308, 343, 382
CviQI GTAC 1 cut(s) 284
DdeI CTNAG 1 cut(s) 374
DpnI GATC 4 cut(s) 130, 140, 236, 260
DpnII GATC 4 cut(s) 128, 138, 234, 258
EaeI YGGCCR 1 cut(s) 159
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 117
Eco32I GATATC 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 32
Eco53kI GAGCTC 1 cut(s) 115
Eco88I CYCGRG 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 115
EcoRV GATATC 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 2 cut(s) 94, 267
FaiI YATR 5 cut(s) 92, 222, 265, 279, 297
FatI CATG 2 cut(s) 90, 263
Fnu4HI GCNGC 9 cut(s) 5, 20, 23, 62, 193, 225, 243, 309, 371
FokI GGATG 3 cut(s) 69, 107, 264
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 9 cut(s) 5, 20, 23, 62, 193, 225, 243, 309, 371
FspBI CTAG 1 cut(s) 395
GlaI GCGC 2 cut(s) 12, 183
GluI GCNGC 9 cut(s) 5, 20, 23, 62, 193, 225, 243, 309, 371
HaeII RGCGCY 2 cut(s) 14, 185
HaeIII GGCC 1 cut(s) 161
HapII CCGG 4 cut(s) 8, 30, 35, 200
HhaI GCGC 2 cut(s) 13, 184
Hin1II CATG 2 cut(s) 94, 267
Hin6I GCGC 2 cut(s) 11, 182
HinP1I GCGC 2 cut(s) 11, 182
HinfI GANTC 1 cut(s) 153
HpaII CCGG 4 cut(s) 8, 30, 35, 200
Hpy188I TCNGA 3 cut(s) 152, 232, 337
Hpy188III TCNNGA 1 cut(s) 376
HpyCH4V TGCA 2 cut(s) 227, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 19, 379
HpyF3I CTNAG 1 cut(s) 374
Hsp92II CATG 2 cut(s) 94, 267
HspAI GCGC 2 cut(s) 11, 182
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
Kzo9I GATC 4 cut(s) 128, 138, 234, 258
LmnI GCTCC 1 cut(s) 340
Lsp1109I GCAGC 6 cut(s) 6, 9, 73, 211, 295, 357
LweI GCATC 2 cut(s) 236, 242
MaeI CTAG 1 cut(s) 395
MaeIII GTNAC 1 cut(s) 316
MalI GATC 4 cut(s) 130, 140, 236, 260
MboI GATC 4 cut(s) 128, 138, 234, 258
MboII GAAGA 2 cut(s) 137, 349
MflI RGATCY 1 cut(s) 234
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 1 cut(s) 302
MnlI CCTC 6 cut(s) 34, 99, 167, 264, 331, 372
MreI CGCCGGCG 1 cut(s) 7
MroNI GCCGGC 1 cut(s) 7
MspI CCGG 4 cut(s) 8, 30, 35, 200
MspR9I CCNGG 1 cut(s) 36
MwoI GCNNNNNNNGC 3 cut(s) 10, 19, 379
NaeI GCCGGC 1 cut(s) 9
NciI CCSGG 1 cut(s) 36
NcoI CCATGG 1 cut(s) 90
NdeII GATC 4 cut(s) 128, 138, 234, 258
NgoMIV GCCGGC 1 cut(s) 7
NlaIII CATG 2 cut(s) 94, 267
NlaIV GGNNCC 1 cut(s) 342
NmuCI GTSAC 1 cut(s) 316
PdiI GCCGGC 1 cut(s) 9
PfeI GAWTC 1 cut(s) 153
PfoI TCCNGGA 1 cut(s) 34
PkrI GCNGC 9 cut(s) 6, 21, 24, 63, 194, 226, 244, 310, 372
Psp124BI GAGCTC 1 cut(s) 117
PspN4I GGNNCC 1 cut(s) 342
PspPI GGNCC 1 cut(s) 32
PsuI RGATCY 1 cut(s) 234
RsaI GTAC 1 cut(s) 285
RsaNI GTAC 1 cut(s) 284
Rsr2I CGGWCCG 1 cut(s) 32
RsrII CGGWCCG 1 cut(s) 32
SacI GAGCTC 1 cut(s) 117
SatI GCNGC 9 cut(s) 5, 20, 23, 62, 193, 225, 243, 309, 371
Sau3AI GATC 4 cut(s) 128, 138, 234, 258
Sau96I GGNCC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 1 cut(s) 117
SetI ASST 1 cut(s) 117
SfaNI GCATC 2 cut(s) 236, 242
SgrAI CRCCGGYG 1 cut(s) 7
SinI GGWCC 1 cut(s) 32
Sse9I AATT 1 cut(s) 302
SsiI CCGC 4 cut(s) 5, 77, 192, 242
SspMI CTAG 1 cut(s) 395
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 34
StyI CCWWGG 1 cut(s) 90
TaqI TCGA 3 cut(s) 111, 137, 257
TasI AATT 1 cut(s) 302
TauI GCSGC 3 cut(s) 7, 195, 245
TfiI GAWTC 1 cut(s) 153
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 6 cut(s) 19, 22, 61, 224, 308, 370
Tsp45I GTSAC 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 32
XspI CTAG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.