pycom08g17810

Polysaccharide lyase family 4, domain III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014889
Physical Location & Seq
Reverse (-)
17930986 .. 17933955
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g17810.6

Sequence Viewer

Length: 1161 bp
ATGTCGGCCCTACCACTCTTGCTATGTTTCTTAGTGGTCATTATGCGGGACAAGATCTGGTGCCAAAATTTAGAGGTGGTGAACCATGGAAGAAGGTTTTTGGCCCTGTGTTTATCTATCTCAATTCTTCCACAATTATCCAAGATCGATCCGTTTTGGCTATGGGAGGATGCTAAAATACAGATGATGACAGAAGTACAAAGCTGGCCATATAGTTTCCCAGCATCTGAGGATTTTCAAAAGTCGGATCAACGGGGTAATGTGAGTGGTAGACTACTTGTCTTGGACAGGTACATATGTGAAGAATACACACCAGCAGGTGGTGCGTACGTTGGTCTTGCCCCACCAGGAGATGTGGGATCATGGCAGAGAGAATGCAAGGACTATCAATTCTGGACAAGAGCAGATGACCGTGGCTATTTTGCTATTAACAACGTGCGTACTGGCAATTATAATCTTTATGCATGGGTGCCTGGGTTTATTGGTGATTACCGGTATGATGCTGCCATTGACATAACCTCAGGTTCTTTTATCGAAATGGGTGATCTTGTGTATGAGCCCCCAAGAGATGGCCCAACATTGTGGGAAATAGGCATTCCTGATCGTTCTGCTGCGGAGTTTTATGTTCCTGATCCTGATCCAAAATATATCAACAAACTTTTCATAAATCATCCTGACAGATTCAGGCAATATGGGCTGTGGAACAGATATGCTGAAATATATCCTGATGAAGACTTAGTTTACACTATCGGTGTTAGTGATTATGCTAAAGATTGGTTCTATGCTCAGGTTCCCAGGAAAACGGACAACGGACATGAAGGAACAACATGGCAGATTAAGTTTGAACTGAAAAATGTGGATCGGAACAGTACCTATAAATTGCGAGTGGCAATTGCATCTGCAACTCTTGCCGAATTGCAGGTTCGGATTAATGATCCAAATTCAAGGCGACCTCTATTTACTAGTGGATTGATAGGGAGGGACAATTCGGTTGCGAGACTTGGAATTCATGGGATTTACTGGCTGTACAACATTAATGTACCAGGCACTCAGCTTGTTGAGGGGAATAATACTTTCTTCTTCAGACAACCAAGATGCACCAGCCCTTTCCAAGCGATTATGTACGATTATATACGATTTGAAGGTCCCCCCGCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

387

Amino Acids

44.56

Weight (kDa)

5.3

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014889)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09910 AT1G09910 AT1G09910
fragaria_vesca FvH4_5g34110 FvH4_5g34110
malus_domestica MD08G1205900.v1.1
prunus_persica Prupe.1G538400_v2.0.a1 Prupe.1G538400_v2.0.a1
pyrus_communis pycom08g17810 pycom15g34900
rosa_chinensis RchiOBHm_Chr7g0233611
rosa_laevigata RLG00000001249
rosa_rugosa Rorug07G0281500 Rorug07G0281600
rosa_samantha Rh7AG438100 Rh7BG410800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 453
AarI CACCTGC 1 cut(s) 308
Acc36I ACCTGC 2 cut(s) 308, 910
AccB1I GGYRCC 2 cut(s) 60, 469
AccB7I CCANNNNNTGG 2 cut(s) 320, 569
AccI GTMKAC 1 cut(s) 271
AciI CCGC 3 cut(s) 46, 614, 1152
AclWI GGATC 7 cut(s) 143, 255, 367, 626, 632, 867, 929
AcoI YGGCCR 1 cut(s) 206
AcsI RAATTY 3 cut(s) 67, 940, 1005
AcuI CTGAAG 1 cut(s) 1066
AfaI GTAC 8 cut(s) 198, 293, 329, 442, 871, 1028, 1041, 1124
AfiI CCNNNNNNNGG 2 cut(s) 320, 569
AgeI ACCGGT 1 cut(s) 492
AgsI TTSAA 4 cut(s) 239, 845, 945, 1142
AhdI GACNNNNNGTC 1 cut(s) 278
AhlI ACTAGT 1 cut(s) 962
AjnI CCWGG 4 cut(s) 346, 472, 794, 1042
AluBI AGCT 2 cut(s) 204, 1054
AluI AGCT 2 cut(s) 204, 1054
Alw26I GTCTC 1 cut(s) 991
AlwI GGATC 7 cut(s) 143, 255, 367, 626, 632, 867, 929
AlwNI CAGNNNCTG 1 cut(s) 227
AoxI GGCC 4 cut(s) 6, 102, 206, 571
ApeKI GCWGC 2 cut(s) 503, 611
ApoI RAATTY 3 cut(s) 67, 940, 1005
AseI ATTAAT 2 cut(s) 930, 1035
AsiGI ACCGGT 1 cut(s) 492
AspS9I GGNCC 4 cut(s) 7, 103, 572, 1145
AsuHPI GGTGA 3 cut(s) 91, 497, 554
AvaII GGWCC 1 cut(s) 1145
AxyI CCTNAGG 1 cut(s) 520
BaeI ACNNNNGTAYC 2 cut(s) 283, 316
BalI TGGCCA 1 cut(s) 208
BanI GGYRCC 2 cut(s) 60, 469
BanII GRGCYC 1 cut(s) 561
BbsI GAAGAC 1 cut(s) 738
BbvI GCAGC 2 cut(s) 490, 598
BccI CCATC 1 cut(s) 563
BciT130I CCWGG 4 cut(s) 348, 474, 796, 1044
BcoDI GTCTC 1 cut(s) 991
BcuI ACTAGT 1 cut(s) 962
BfaI CTAG 1 cut(s) 963
BfuAI ACCTGC 2 cut(s) 308, 910
BglII AGATCT 1 cut(s) 54
BisI GCNGC 2 cut(s) 504, 612
BlsI GCNGC 2 cut(s) 505, 613
Bme1390I CCNGG 4 cut(s) 348, 474, 796, 1044
Bme18I GGWCC 1 cut(s) 1145
BmeRI GACNNNNNGTC 1 cut(s) 278
BmgT120I GGNCC 4 cut(s) 7, 103, 572, 1145
BmiI GGNNCC 4 cut(s) 62, 471, 792, 1147
BmrFI CCNGG 4 cut(s) 348, 474, 796, 1044
BmsI GCATC 5 cut(s) 160, 233, 490, 905, 1085
BpiI GAAGAC 1 cut(s) 738
Bpu10I CCTNAGC 1 cut(s) 786
Bsa29I ATCGAT 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 636
BsaJI CCNNGG 4 cut(s) 85, 412, 473, 794
BsaWI WCCGGW 1 cut(s) 492
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 569
Bse118I RCCGGY 1 cut(s) 492
Bse1I ACTGG 2 cut(s) 448, 1025
Bse21I CCTNAGG 1 cut(s) 520
Bse8I GATNNNNATC 1 cut(s) 636
BseBI CCWGG 4 cut(s) 348, 474, 796, 1044
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 4 cut(s) 85, 412, 473, 794
BseGI GGATG 2 cut(s) 175, 670
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 2 cut(s) 320, 569
BseMII CTCAG 4 cut(s) 219, 534, 800, 1064
BseNI ACTGG 2 cut(s) 448, 1025
BseXI GCAGC 2 cut(s) 490, 598
BseYI CCCAGC 1 cut(s) 220
BshFI GGCC 4 cut(s) 8, 104, 208, 573
BshNI GGYRCC 2 cut(s) 60, 469
BshTI ACCGGT 1 cut(s) 492
BshVI ATCGAT 1 cut(s) 147
BsiSI CCGG 1 cut(s) 493
BsiWI CGTACG 1 cut(s) 327
BslFI GGGAC 3 cut(s) 62, 995, 1131
BslI CCNNNNNNNGG 2 cut(s) 320, 569
BsmAI GTCTC 1 cut(s) 991
BsmFI GGGAC 3 cut(s) 62, 995, 1131
BsmI GAATGC 2 cut(s) 380, 594
BsnI GGCC 4 cut(s) 8, 104, 208, 573
Bsp1286I GDGCHC 1 cut(s) 561
Bsp1407I TGTACA 1 cut(s) 1026
Bsp19I CCATGG 1 cut(s) 85
BspACI CCGC 3 cut(s) 46, 614, 1152
BspANI GGCC 4 cut(s) 8, 104, 208, 573
BspCNI CTCAG 4 cut(s) 220, 533, 799, 1063
BspDI ATCGAT 1 cut(s) 147
BspLI GGNNCC 4 cut(s) 62, 471, 792, 1147
BspMI ACCTGC 2 cut(s) 308, 910
BspPI GGATC 7 cut(s) 143, 255, 367, 626, 632, 867, 929
BspT107I GGYRCC 2 cut(s) 60, 469
BsrFI RCCGGY 1 cut(s) 492
BsrGI TGTACA 1 cut(s) 1026
BsrI ACTGG 2 cut(s) 448, 1025
BssAI RCCGGY 1 cut(s) 492
BssECI CCNNGG 4 cut(s) 85, 412, 473, 794
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 4 cut(s) 348, 474, 796, 1044
Bst4CI ACNGT 2 cut(s) 413, 869
BstAPI GCANNNNNTGC 2 cut(s) 323, 908
BstAUI TGTACA 1 cut(s) 1026
BstC8I GCNNGC 1 cut(s) 206
BstDEI CTNAG 6 cut(s) 31, 228, 520, 736, 786, 1050
BstDSI CCRYGG 2 cut(s) 85, 412
BstF5I GGATG 2 cut(s) 175, 670
BstMAI GTCTC 1 cut(s) 991
BstMWI GCNNNNNNNGC 3 cut(s) 323, 694, 908
BstNI CCWGG 4 cut(s) 348, 474, 796, 1044
BstSCI CCNGG 4 cut(s) 346, 472, 794, 1042
BstV1I GCAGC 2 cut(s) 490, 598
BstV2I GAAGAC 1 cut(s) 738
BstX2I RGATCY 1 cut(s) 54
BstXI CCANNNNNNTGG 1 cut(s) 582
BstYI RGATCY 1 cut(s) 54
Bsu15I ATCGAT 1 cut(s) 147
Bsu36I CCTNAGG 1 cut(s) 520
BsuRI GGCC 4 cut(s) 8, 104, 208, 573
BsuTUI ATCGAT 1 cut(s) 147
BtgI CCRYGG 2 cut(s) 85, 412
BtsCI GGATG 2 cut(s) 175, 670
BveI ACCTGC 2 cut(s) 308, 910
Cac8I GCNNGC 1 cut(s) 206
CaiI CAGNNNCTG 1 cut(s) 227
Cfr10I RCCGGY 1 cut(s) 492
Cfr13I GGNCC 4 cut(s) 7, 103, 572, 1145
ClaI ATCGAT 1 cut(s) 147
Csp6I GTAC 8 cut(s) 197, 292, 328, 441, 870, 1027, 1040, 1123
CspAI ACCGGT 1 cut(s) 492
CviAII CATG 6 cut(s) 86, 363, 465, 815, 828, 1010
CviQI GTAC 8 cut(s) 197, 292, 328, 441, 870, 1027, 1040, 1123
DdeI CTNAG 6 cut(s) 31, 228, 520, 736, 786, 1050
DriI GACNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 1 cut(s) 206
Eam1105I GACNNNNNGTC 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 1145
Eco57I CTGAAG 1 cut(s) 1066
Eco81I CCTNAGG 1 cut(s) 520
EcoO109I RGGNCCY 1 cut(s) 1145
EcoRI GAATTC 1 cut(s) 1005
EcoRII CCWGG 4 cut(s) 346, 472, 794, 1042
EcoT14I CCWWGG 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 6 cut(s) 89, 366, 468, 818, 831, 1013
FaqI GGGAC 3 cut(s) 62, 995, 1131
FatI CATG 6 cut(s) 85, 362, 464, 814, 827, 1009
FauI CCCGC 2 cut(s) 39, 1159
FauNDI CATATG 1 cut(s) 296
FblI GTMKAC 1 cut(s) 271
Fnu4HI GCNGC 2 cut(s) 504, 612
FokI GGATG 2 cut(s) 182, 657
FriOI GRGCYC 1 cut(s) 561
Fsp4HI GCNGC 2 cut(s) 504, 612
FspBI CTAG 1 cut(s) 963
GluI GCNGC 2 cut(s) 504, 612
GsaI CCCAGC 1 cut(s) 224
HaeIII GGCC 4 cut(s) 8, 104, 208, 573
HapII CCGG 1 cut(s) 493
Hin1II CATG 6 cut(s) 89, 366, 468, 818, 831, 1013
HinfI GANTC 1 cut(s) 681
HpaII CCGG 1 cut(s) 493
HphI GGTGA 3 cut(s) 91, 497, 554
Hpy166II GTNNAC 3 cut(s) 82, 272, 742
Hpy188I TCNGA 5 cut(s) 229, 247, 864, 927, 1085
Hpy188III TCNNGA 6 cut(s) 394, 599, 629, 635, 674, 725
Hpy8I GTNNAC 3 cut(s) 82, 272, 742
HpyAV CCTTC 3 cut(s) 87, 812, 1136
HpyCH4III ACNGT 2 cut(s) 413, 869
HpyCH4IV ACGT 2 cut(s) 330, 435
HpyCH4V TGCA 6 cut(s) 378, 464, 896, 902, 919, 1098
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 694, 908
HpyF3I CTNAG 6 cut(s) 31, 228, 520, 736, 786, 1050
HpySE526I ACGT 2 cut(s) 330, 435
Hsp92II CATG 6 cut(s) 89, 366, 468, 818, 831, 1013
Lsp1109I GCAGC 2 cut(s) 490, 598
LweI GCATC 5 cut(s) 160, 233, 490, 905, 1085
MaeI CTAG 1 cut(s) 963
MaeII ACGT 2 cut(s) 330, 435
MboII GAAGA 6 cut(s) 102, 119, 314, 743, 1069, 1072
MfeI CAATTG 1 cut(s) 891
MflI RGATCY 1 cut(s) 54
MhlI GDGCHC 1 cut(s) 561
MlsI TGGCCA 1 cut(s) 208
MluNI TGGCCA 1 cut(s) 208
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 7 cut(s) 67, 160, 223, 529, 963, 972, 1054
Mox20I TGGCCA 1 cut(s) 208
Mph1103I ATGCAT 1 cut(s) 466
MscI TGGCCA 1 cut(s) 208
MseI TTAA 4 cut(s) 429, 837, 930, 1035
Msp20I TGGCCA 1 cut(s) 208
MspI CCGG 1 cut(s) 493
MspR9I CCNGG 4 cut(s) 348, 474, 796, 1044
MunI CAATTG 1 cut(s) 891
Mva1269I GAATGC 2 cut(s) 380, 594
MvaI CCWGG 4 cut(s) 348, 474, 796, 1044
MwoI GCNNNNNNNGC 3 cut(s) 323, 694, 908
NcoI CCATGG 1 cut(s) 85
NdeI CATATG 1 cut(s) 296
NlaIII CATG 6 cut(s) 89, 366, 468, 818, 831, 1013
NlaIV GGNNCC 4 cut(s) 62, 471, 792, 1147
NsiI ATGCAT 1 cut(s) 466
PaqCI CACCTGC 1 cut(s) 308
PctI GAATGC 2 cut(s) 380, 594
PfeI GAWTC 1 cut(s) 681
Pfl23II CGTACG 1 cut(s) 327
PflMI CCANNNNNTGG 2 cut(s) 320, 569
PinAI ACCGGT 1 cut(s) 492
PkrI GCNGC 2 cut(s) 505, 613
PpuMI RGGWCCY 1 cut(s) 1145
PshBI ATTAAT 2 cut(s) 930, 1035
PsiI TTATAA 1 cut(s) 453
Psp5II RGGWCCY 1 cut(s) 1145
Psp6I CCWGG 4 cut(s) 346, 472, 794, 1042
PspFI CCCAGC 1 cut(s) 220
PspGI CCWGG 4 cut(s) 346, 472, 794, 1042
PspLI CGTACG 1 cut(s) 327
PspN4I GGNNCC 4 cut(s) 62, 471, 792, 1147
PspPI GGNCC 4 cut(s) 7, 103, 572, 1145
PspPPI RGGWCCY 1 cut(s) 1145
PstNI CAGNNNCTG 1 cut(s) 227
PsuI RGATCY 1 cut(s) 54
RsaI GTAC 8 cut(s) 198, 293, 329, 442, 871, 1028, 1041, 1124
RsaNI GTAC 8 cut(s) 197, 292, 328, 441, 870, 1027, 1040, 1123
SaqAI TTAA 4 cut(s) 429, 837, 930, 1035
SatI GCNGC 2 cut(s) 504, 612
Sau96I GGNCC 4 cut(s) 7, 103, 572, 1145
ScrFI CCNGG 4 cut(s) 348, 474, 796, 1044
SduI GDGCHC 1 cut(s) 561
SfaNI GCATC 5 cut(s) 160, 233, 490, 905, 1085
SinI GGWCC 1 cut(s) 1145
SpeI ACTAGT 1 cut(s) 962
SsiI CCGC 3 cut(s) 46, 614, 1152
SspMI CTAG 1 cut(s) 963
StyD4I CCNGG 4 cut(s) 346, 472, 794, 1042
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 413, 869
TaiI ACGT 2 cut(s) 333, 438
TaqI TCGA 2 cut(s) 147, 534
TatI WGTACW 2 cut(s) 196, 1026
TfiI GAWTC 1 cut(s) 681
Tru1I TTAA 4 cut(s) 429, 837, 930, 1035
Tru9I TTAA 4 cut(s) 429, 837, 930, 1035
TseI GCWGC 2 cut(s) 503, 611
TspDTI ATGAA 4 cut(s) 652, 744, 831, 998
TspGWI ACGGA 3 cut(s) 141, 818, 825
Van91I CCANNNNNTGG 2 cut(s) 320, 569
VpaK11BI GGWCC 1 cut(s) 1145
VspI ATTAAT 2 cut(s) 930, 1035
XapI RAATTY 3 cut(s) 67, 940, 1005
XmiI GTMKAC 1 cut(s) 271
XspI CTAG 1 cut(s) 963
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.