Rh7AG438100

Polysaccharide lyase family 4, domain III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014889
Physical Location & Seq
Forward (+)
59080716 .. 59086615
5900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG438100.1

Sequence Viewer

Length: 1431 bp
ATGGGCATCCCCGTAATTTCAGAACCCCACCTCTCTCTCTCTCTCTCTCAAAGATTCAAATCCAGAGTTGTTTCGATAGGTTTCAATAGACAACAAGAGTCTCAGACCATGTCAGGAGCAGGAGTGCGGTTACATAATCTGGGTCCCCATGTGGTTATGGATAATGGCATAGTCCAAGTCACCTTATCAAACCCAGATGGTATTGTCACCGGAATACGCTATAATGGGATTGACAATTTGCTTGAACCTCTCAATCCTGAACATAATAGAGGGTATTGGGACCTTGTCTGGAGTGGACCAGGAGTTAAGGGAACATTTGATGTGATAAAAGGTACTACTTTTAGGGTTATTGTGGAGAATGAGGAACAGGTCGAGCTCTCATTTACAAGAACGTGGGATCCCTCTCTTGAGGGGAAATATGTTCCCTTAAACATAGACAAAAGGTTTGTAATGCTTCGTGGTTCCTCGGGCTTCTACTCCTATGCAATTTATGAGCACCTGAAGGATTGGCCTGATTTTGAGATTGGTGAAACCAGAATAACTTTCAAACTGAGAAAAGACAAGTTCCAATACATGGCTATATCAGACAACAGGCAAAGATACATGCCCCTACCTGATGACCGGATGCCAGGAAGATGCCAGCCCCTGGCTTACCCAGAAGCTGTCCTACTTCTGAATCCCAAGATGCAAGAGCTTGCCGGAGAGGTAGATGACAAGTACCAGTACTCATGTGTGAACAAAGACAATCAAGTTCATGGTTGGATATGCACCAACCCACCAGTTGGCTTTTGGCAGATCACACCAAGTGATGAATTCCGGTCTGGTGGACCTCTGAAACAGAATCTAACCTCACACGTTGGCCCCACCACTCTTGCAATGTTTCATAGTTCTCATTATGCGGGACAAGATCTGGTGCCAAAATTTAGAGGTGGTGAACCATGGAAGAAGGTTTTTGGGCCTGTTTTTATCTATCTCAATTCTGCGGGAATTAGAGATGATCCATTCTTTTTGTGGGAGGACGCTAAAATTAAGATGATGGCAGAAGTCCAAAGCTGGCCATATAGTTTTCCAGCTTCTGAGGATTTCCAAAAGTCAGATCAACGGGGTAATGTGAGTGGTAGACTACTTGTCTTGGACAGGTATGTTAGTGAAGAATATATACCAGCAAATGATGCATATGTTGGTTTGGCTCCACCAGGAGATGTAGGATCATGGCAACGAGAATGCAAGGACTATCAATTCTGGTCGAGGGCAGATAACAGAGGTTACTTTTGCATTAACAACATATGTACTGGCACGTACAATCTTTATGCATGGGTCCCTGGTTTTATTGGAGATTACCGGTATGAAGCAGTCATTAACATAACCTCAGGTGTTGTTCTCAACTTTCTAATTCCAAGTCAATTGTTTCCTTTCTTTCATTTGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

476

Amino Acids

54.32

Weight (kDa)

5.86

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 43 - 236 1.4e-93 Rhamnogalacturonate lyase family
fn3_3 PF14686 390 - 458 7.5e-25 Polysaccharide lyase family 4, domain II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014889)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09910 AT1G09910 AT1G09910
fragaria_vesca FvH4_5g34110 FvH4_5g34110
malus_domestica MD08G1205900.v1.1
prunus_persica Prupe.1G538400_v2.0.a1 Prupe.1G538400_v2.0.a1
pyrus_communis pycom08g17810 pycom15g34900
rosa_chinensis RchiOBHm_Chr7g0233611
rosa_laevigata RLG00000001249
rosa_rugosa Rorug07G0281500 Rorug07G0281600
rosa_samantha Rh7AG438100 Rh7BG410800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 913
AccB7I CCANNNNNTGG 3 cut(s) 574, 646, 782
AccI GTMKAC 1 cut(s) 1120
AciI CCGC 3 cut(s) 127, 899, 983
AclWI GGATC 4 cut(s) 392, 405, 992, 1216
AcoI YGGCCR 1 cut(s) 1055
AcsI RAATTY 2 cut(s) 812, 920
AcuI CTGAAG 1 cut(s) 521
AdeI CACNNNGTG 1 cut(s) 806
AfaI GTAC 5 cut(s) 334, 719, 725, 1291, 1301
AfiI CCNNNNNNNGG 3 cut(s) 574, 646, 782
AflIII ACRYGT 1 cut(s) 853
AgeI ACCGGT 1 cut(s) 1341
AgsI TTSAA 4 cut(s) 58, 85, 245, 547
AhdI GACNNNNNGTC 1 cut(s) 1127
AjnI CCWGG 5 cut(s) 298, 628, 645, 1195, 1321
AloI GAACNNNNNNTCC 2 cut(s) 382, 414
AluBI AGCT 5 cut(s) 376, 662, 694, 1053, 1073
AluI AGCT 5 cut(s) 376, 662, 694, 1053, 1073
Alw21I GWGCWC 2 cut(s) 378, 498
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 4 cut(s) 392, 405, 992, 1216
AlwNI CAGNNNCTG 3 cut(s) 646, 662, 1076
Ama87I CYCGRG 1 cut(s) 466
AoxI GGCC 4 cut(s) 509, 859, 956, 1055
ApoI RAATTY 2 cut(s) 812, 920
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AsiGI ACCGGT 1 cut(s) 1341
AspS9I GGNCC 7 cut(s) 143, 280, 296, 827, 860, 956, 1318
AsuHPI GGTGA 4 cut(s) 172, 199, 539, 944
AvaI CYCGRG 1 cut(s) 466
AvaII GGWCC 5 cut(s) 143, 280, 296, 827, 1318
AxyI CCTNAGG 1 cut(s) 1369
BalI TGGCCA 1 cut(s) 1057
BamHI GGATCC 1 cut(s) 397
BanI GGYRCC 1 cut(s) 913
BanII GRGCYC 1 cut(s) 378
BarI GAAGNNNNNNTAC 4 cut(s) 651, 683, 1143, 1175
Bbv12I GWGCWC 2 cut(s) 378, 498
BccI CCATC 2 cut(s) 191, 1030
BciT130I CCWGG 5 cut(s) 300, 630, 647, 1197, 1323
BcoDI GTCTC 1 cut(s) 105
BglII AGATCT 1 cut(s) 907
BmcAI AGTACT 1 cut(s) 725
Bme1390I CCNGG 5 cut(s) 300, 630, 647, 1197, 1323
Bme18I GGWCC 5 cut(s) 143, 280, 296, 827, 1318
BmeRI GACNNNNNGTC 1 cut(s) 1127
BmeT110I CYCGRG 1 cut(s) 466
BmgT120I GGNCC 7 cut(s) 143, 280, 296, 827, 860, 956, 1318
BmrFI CCNGG 5 cut(s) 300, 630, 647, 1197, 1323
BmsI GCATC 5 cut(s) 15, 615, 626, 675, 1162
BpmI CTGGAG 1 cut(s) 310
BpuEI CTTGAG 1 cut(s) 428
BsaAI YACGTR 1 cut(s) 1299
BsaBI GATNNNNATC 1 cut(s) 58
BsaJI CCNNGG 4 cut(s) 465, 645, 938, 1321
BsaWI WCCGGW 4 cut(s) 209, 621, 816, 1341
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 3 cut(s) 574, 646, 782
Bse118I RCCGGY 1 cut(s) 1341
Bse1I ACTGG 3 cut(s) 721, 779, 1297
Bse21I CCTNAGG 1 cut(s) 1369
Bse3DI GCAATG 1 cut(s) 882
Bse8I GATNNNNATC 1 cut(s) 58
BseBI CCWGG 5 cut(s) 300, 630, 647, 1197, 1323
BseDI CCNNGG 4 cut(s) 465, 645, 938, 1321
BseGI GGATG 2 cut(s) 6, 630
BseJI GATNNNNATC 1 cut(s) 58
BseLI CCNNNNNNNGG 3 cut(s) 574, 646, 782
BseMI GCAATG 1 cut(s) 882
BseMII CTCAG 4 cut(s) 116, 542, 1068, 1383
BseNI ACTGG 3 cut(s) 721, 779, 1297
BshFI GGCC 4 cut(s) 511, 861, 958, 1057
BshNI GGYRCC 1 cut(s) 913
BshTI ACCGGT 1 cut(s) 1341
BsiHKAI GWGCWC 2 cut(s) 378, 498
BsiHKCI CYCGRG 1 cut(s) 466
BsiSI CCGG 5 cut(s) 210, 622, 699, 817, 1342
BslFI GGGAC 4 cut(s) 129, 293, 915, 1304
BslI CCNNNNNNNGG 3 cut(s) 574, 646, 782
BsmAI GTCTC 1 cut(s) 105
BsmFI GGGAC 4 cut(s) 129, 293, 915, 1304
BsmI GAATGC 1 cut(s) 1229
BsnI GGCC 4 cut(s) 511, 861, 958, 1057
BsoBI CYCGRG 1 cut(s) 466
Bsp1286I GDGCHC 2 cut(s) 378, 498
Bsp143I GATC 6 cut(s) 397, 795, 907, 997, 1096, 1208
Bsp19I CCATGG 1 cut(s) 938
BspACI CCGC 3 cut(s) 127, 899, 983
BspANI GGCC 4 cut(s) 511, 861, 958, 1057
BspCNI CTCAG 4 cut(s) 115, 543, 1069, 1382
BspPI GGATC 4 cut(s) 392, 405, 992, 1216
BspT107I GGYRCC 1 cut(s) 913
BsrDI GCAATG 1 cut(s) 882
BsrFI RCCGGY 1 cut(s) 1341
BsrI ACTGG 3 cut(s) 721, 779, 1297
BssAI RCCGGY 1 cut(s) 1341
BssECI CCNNGG 4 cut(s) 465, 645, 938, 1321
BssMI GATC 6 cut(s) 397, 795, 907, 997, 1096, 1208
BssT1I CCWWGG 1 cut(s) 938
Bst2UI CCWGG 5 cut(s) 300, 630, 647, 1197, 1323
BstAPI GCANNNNNTGC 1 cut(s) 1172
BstBAI YACGTR 1 cut(s) 1299
BstC8I GCNNGC 3 cut(s) 641, 696, 1055
BstDEI CTNAG 4 cut(s) 102, 551, 1077, 1369
BstDSI CCRYGG 1 cut(s) 938
BstF5I GGATG 2 cut(s) 6, 630
BstKTI GATC 6 cut(s) 400, 798, 910, 1000, 1099, 1211
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 6 cut(s) 397, 795, 907, 997, 1096, 1208
BstMWI GCNNNNNNNGC 1 cut(s) 1172
BstNI CCWGG 5 cut(s) 300, 630, 647, 1197, 1323
BstNSI RCATGY 1 cut(s) 607
BstSCI CCNGG 5 cut(s) 298, 628, 645, 1195, 1321
BstX2I RGATCY 2 cut(s) 397, 907
BstYI RGATCY 2 cut(s) 397, 907
Bsu36I CCTNAGG 1 cut(s) 1369
BsuRI GGCC 4 cut(s) 511, 861, 958, 1057
BtgI CCRYGG 1 cut(s) 938
BtsCI GGATG 2 cut(s) 6, 630
Cac8I GCNNGC 3 cut(s) 641, 696, 1055
CaiI CAGNNNCTG 3 cut(s) 646, 662, 1076
Cfr10I RCCGGY 1 cut(s) 1341
Cfr13I GGNCC 7 cut(s) 143, 280, 296, 827, 860, 956, 1318
CseI GACGC 1 cut(s) 1028
Csp6I GTAC 5 cut(s) 333, 718, 724, 1290, 1300
CspAI ACCGGT 1 cut(s) 1341
CspCI CAANNNNNGTGG 2 cut(s) 853, 888
CviAII CATG 9 cut(s) 109, 149, 574, 604, 729, 755, 939, 1212, 1314
CviQI GTAC 5 cut(s) 333, 718, 724, 1290, 1300
DdeI CTNAG 4 cut(s) 102, 551, 1077, 1369
DpnI GATC 6 cut(s) 399, 797, 909, 999, 1098, 1210
DpnII GATC 6 cut(s) 397, 795, 907, 997, 1096, 1208
DraIII CACNNNGTG 1 cut(s) 806
DriI GACNNNNNGTC 1 cut(s) 1127
EaeI YGGCCR 1 cut(s) 1055
Eam1105I GACNNNNNGTC 1 cut(s) 1127
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 938
Eco24I GRGCYC 1 cut(s) 378
Eco47I GGWCC 5 cut(s) 143, 280, 296, 827, 1318
Eco53kI GAGCTC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 521
Eco81I CCTNAGG 1 cut(s) 1369
Eco88I CYCGRG 1 cut(s) 466
EcoICRI GAGCTC 1 cut(s) 376
EcoO109I RGGNCCY 3 cut(s) 143, 280, 1318
EcoRI GAATTC 1 cut(s) 812
EcoRII CCWGG 5 cut(s) 298, 628, 645, 1195, 1321
EcoT14I CCWWGG 1 cut(s) 938
EcoT22I ATGCAT 2 cut(s) 1177, 1315
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 938
FaeI CATG 9 cut(s) 112, 152, 577, 607, 732, 758, 942, 1215, 1317
FaqI GGGAC 4 cut(s) 129, 293, 915, 1304
FatI CATG 9 cut(s) 108, 148, 573, 603, 728, 754, 938, 1211, 1313
FauI CCCGC 2 cut(s) 892, 976
FauNDI CATATG 2 cut(s) 1177, 1286
FblI GTMKAC 1 cut(s) 1120
FokI GGATG 1 cut(s) 637
FriOI GRGCYC 1 cut(s) 378
GsuI CTGGAG 1 cut(s) 310
HaeIII GGCC 4 cut(s) 511, 861, 958, 1057
HapII CCGG 5 cut(s) 210, 622, 699, 817, 1342
HgaI GACGC 1 cut(s) 1028
Hin1II CATG 9 cut(s) 112, 152, 577, 607, 732, 758, 942, 1215, 1317
HinfI GANTC 4 cut(s) 54, 98, 676, 841
HpaII CCGG 5 cut(s) 210, 622, 699, 817, 1342
HphI GGTGA 4 cut(s) 172, 199, 539, 944
Hpy166II GTNNAC 5 cut(s) 296, 736, 827, 935, 1121
Hpy188I TCNGA 7 cut(s) 22, 105, 586, 675, 834, 1078, 1096
Hpy188III TCNNGA 5 cut(s) 63, 114, 257, 289, 407
Hpy8I GTNNAC 5 cut(s) 296, 736, 827, 935, 1121
HpyAV CCTTC 2 cut(s) 496, 940
HpyCH4IV ACGT 3 cut(s) 392, 855, 1298
HpyCH4V TGCA 8 cut(s) 485, 688, 768, 875, 1175, 1227, 1275, 1313
HpyF10VI GCNNNNNNNGC 1 cut(s) 1172
HpyF3I CTNAG 4 cut(s) 102, 551, 1077, 1369
HpySE526I ACGT 3 cut(s) 392, 855, 1298
Hsp92II CATG 9 cut(s) 112, 152, 577, 607, 732, 758, 942, 1215, 1317
KflI GGGWCCC 2 cut(s) 143, 1318
Kzo9I GATC 6 cut(s) 397, 795, 907, 997, 1096, 1208
LmnI GCTCC 2 cut(s) 116, 1195
LweI GCATC 5 cut(s) 15, 615, 626, 675, 1162
MaeII ACGT 3 cut(s) 392, 855, 1298
MaeIII GTNAC 4 cut(s) 129, 178, 205, 1265
MalI GATC 6 cut(s) 399, 797, 909, 999, 1098, 1210
MboI GATC 6 cut(s) 397, 795, 907, 997, 1096, 1208
MboII GAAGA 3 cut(s) 645, 955, 1163
MfeI CAATTG 1 cut(s) 1403
MflI RGATCY 2 cut(s) 397, 907
MhlI GDGCHC 2 cut(s) 378, 498
MlsI TGGCCA 1 cut(s) 1057
MluNI TGGCCA 1 cut(s) 1057
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 1 cut(s) 740
Mox20I TGGCCA 1 cut(s) 1057
Mph1103I ATGCAT 2 cut(s) 1177, 1315
MscI TGGCCA 1 cut(s) 1057
MseI TTAA 6 cut(s) 306, 428, 1029, 1278, 1359, 1429
Msp20I TGGCCA 1 cut(s) 1057
MspI CCGG 5 cut(s) 210, 622, 699, 817, 1342
MspR9I CCNGG 5 cut(s) 300, 630, 647, 1197, 1323
MunI CAATTG 1 cut(s) 1403
Mva1269I GAATGC 1 cut(s) 1229
MvaI CCWGG 5 cut(s) 300, 630, 647, 1197, 1323
MwoI GCNNNNNNNGC 1 cut(s) 1172
NcoI CCATGG 1 cut(s) 938
NdeI CATATG 2 cut(s) 1177, 1286
NdeII GATC 6 cut(s) 397, 795, 907, 997, 1096, 1208
NlaIII CATG 9 cut(s) 112, 152, 577, 607, 732, 758, 942, 1215, 1317
NmuCI GTSAC 2 cut(s) 178, 205
NsiI ATGCAT 2 cut(s) 1177, 1315
NspI RCATGY 1 cut(s) 607
PctI GAATGC 1 cut(s) 1229
PfeI GAWTC 3 cut(s) 54, 676, 841
PflFI GACNNNGTC 2 cut(s) 109, 284
PflMI CCANNNNNTGG 3 cut(s) 574, 646, 782
PinAI ACCGGT 1 cut(s) 1341
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
Ppu21I YACGTR 1 cut(s) 1299
PpuMI RGGWCCY 3 cut(s) 143, 280, 1318
Psp124BI GAGCTC 1 cut(s) 378
Psp5II RGGWCCY 3 cut(s) 143, 280, 1318
Psp6I CCWGG 5 cut(s) 298, 628, 645, 1195, 1321
PspGI CCWGG 5 cut(s) 298, 628, 645, 1195, 1321
PspPI GGNCC 7 cut(s) 143, 280, 296, 827, 860, 956, 1318
PspPPI RGGWCCY 3 cut(s) 143, 280, 1318
PstNI CAGNNNCTG 3 cut(s) 646, 662, 1076
PsuI RGATCY 2 cut(s) 397, 907
PsyI GACNNNGTC 2 cut(s) 109, 284
RsaI GTAC 5 cut(s) 334, 719, 725, 1291, 1301
RsaNI GTAC 5 cut(s) 333, 718, 724, 1290, 1300
SacI GAGCTC 1 cut(s) 378
SaqAI TTAA 6 cut(s) 306, 428, 1029, 1278, 1359, 1429
Sau3AI GATC 6 cut(s) 397, 795, 907, 997, 1096, 1208
Sau96I GGNCC 7 cut(s) 143, 280, 296, 827, 860, 956, 1318
ScaI AGTACT 1 cut(s) 725
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 5 cut(s) 300, 630, 647, 1197, 1323
SduI GDGCHC 2 cut(s) 378, 498
SfaNI GCATC 5 cut(s) 15, 615, 626, 675, 1162
SinI GGWCC 5 cut(s) 143, 280, 296, 827, 1318
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
SsiI CCGC 3 cut(s) 127, 899, 983
SstI GAGCTC 1 cut(s) 378
StyD4I CCNGG 5 cut(s) 298, 628, 645, 1195, 1321
StyI CCWWGG 1 cut(s) 938
TaiI ACGT 3 cut(s) 395, 858, 1301
TaqI TCGA 3 cut(s) 74, 372, 1247
TatI WGTACW 2 cut(s) 723, 1289
TfiI GAWTC 3 cut(s) 54, 676, 841
Tru1I TTAA 6 cut(s) 306, 428, 1029, 1278, 1359, 1429
Tru9I TTAA 6 cut(s) 306, 428, 1029, 1278, 1359, 1429
TseFI GTSAC 2 cut(s) 178, 205
Tsp45I GTSAC 2 cut(s) 178, 205
TspDTI ATGAA 5 cut(s) 743, 825, 872, 1362, 1409
Tth111I GACNNNGTC 2 cut(s) 109, 284
Van91I CCANNNNNTGG 3 cut(s) 574, 646, 782
VpaK11BI GGWCC 5 cut(s) 143, 280, 296, 827, 1318
XapI RAATTY 2 cut(s) 812, 920
XceI RCATGY 1 cut(s) 607
XcmI CCANNNNNNNNNTGG 2 cut(s) 786, 1008
XmiI GTMKAC 1 cut(s) 1120
ZrmI AGTACT 1 cut(s) 725
Zsp2I ATGCAT 2 cut(s) 1177, 1315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.