pycom15g34900

Polysaccharide lyase family 4, domain III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014889
Physical Location & Seq
Reverse (-)
34723326 .. 34726048
2723 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g34900.7

Sequence Viewer

Length: 1173 bp
ATGTCGGCCCTACCACTCTTGCTATGTTTCTTAGTAGTCATTATGCGGGACAAGATCTGGTGCCAAAATTTAGGGGTGGCGAACCATGGAAGAAGGTTTTCGGCCCTGTTTTTATCTATCTCAATTCTTCCACAATTAAAGATCGATCCGTTTTGGCTATGGGAGGATGCTAAAATACAGATGATGACAGAAGTACAAAGCTGGCCATATAGTTTCCCCGCATCTGAGGATTTCCAAAAGTCTGGTCAACGGGGTAATGTGAGTGGTAGACTACTTGTCTTGGACAGGTACATAAGTGAAGAATACACACCTGCAAATGGTGCGTATGTTGGTCTTGCCCCACCAGGAGATGCAGGATCATGGCAAAGAGAATGCAAGGACTATCAATTCTGGACAAGGGCAGATGACCGTGGTTATTTTTCTATTAACAATGTGCGTACTGGCACTTACAATCTTTATGCATGGGTACCTGGTTTTATTGGAGATTACCGGTATGATGCTGTCATTAACATAACCTCAGGTTCTTTTATCGAAATGGGTGATCTTGTGTATGAACCTCCAAGACATGGGCCAACATTGTGGGAAATAGGCATTCCTGATCGTTCTGCTGCAGAGTTTTATGTTCCTGATCCTGATCCAAAGTATATCAACAAACTTTTCATAGATCATCCTGACAGGTTCAGGCAATATGGGCTCTGGAACAGATATGCTGAATTATATCCCGATGAAGACTTGGTTTACATTGTCGGTGTTAGTGATTATGCTAAAGATTGGTTCTTTGCTCAGGTTCCCAGGAAAACAGACAACGGACATGAAGGAACAACATGGCAGATCAAGTTTGAACTGAAAAATGTGGATCGGAACGGTAGCTATAAATTGCGAGTTGCAATTGCATCTGCAACTCTTGCTGAATTGCAGGTTCGGATTAACGATCCAGATTCAAGGCGACCTCTATTTACTAGTGGATTGATAGGGAGGGATAACTCGGTTGCAAGACATGGAATCCATGGGATTTACTGGCTGTACAACATTAACGTACCAGGCGCTCAGCTTGTTGAGGGGAATAATACTTTCTTCTTTAGACAACCAAGATGCACCAGTCCTTTCCAAGGGATTATGTACGATTATATACGGTTTGAAGGTCCCCCACCTTGTAGTTTCAGTTCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

391

Amino Acids

44.83

Weight (kDa)

5.76

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014889)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09910 AT1G09910 AT1G09910
fragaria_vesca FvH4_5g34110 FvH4_5g34110
malus_domestica MD08G1205900.v1.1
prunus_persica Prupe.1G538400_v2.0.a1 Prupe.1G538400_v2.0.a1
pyrus_communis pycom08g17810 pycom15g34900
rosa_chinensis RchiOBHm_Chr7g0233611
rosa_laevigata RLG00000001249
rosa_rugosa Rorug07G0281500 Rorug07G0281600
rosa_samantha Rh7AG438100 Rh7BG410800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 319
Acc36I ACCTGC 2 cut(s) 319, 907
Acc65I GGTACC 1 cut(s) 466
AccB1I GGYRCC 2 cut(s) 60, 466
AccB7I CCANNNNNTGG 1 cut(s) 566
AccI GTMKAC 1 cut(s) 268
AciI CCGC 2 cut(s) 46, 219
AclWI GGATC 6 cut(s) 140, 364, 623, 629, 864, 926
AcoI YGGCCR 1 cut(s) 203
AcsI RAATTY 1 cut(s) 67
AfaI GTAC 7 cut(s) 195, 290, 439, 468, 1025, 1038, 1121
AfiI CCNNNNNNNGG 3 cut(s) 317, 566, 1109
AgeI ACCGGT 1 cut(s) 489
AgsI TTSAA 3 cut(s) 842, 942, 1139
AhdI GACNNNNNGTC 1 cut(s) 275
AhlI ACTAGT 1 cut(s) 959
AjnI CCWGG 4 cut(s) 343, 469, 791, 1039
AluBI AGCT 3 cut(s) 201, 870, 1051
AluI AGCT 3 cut(s) 201, 870, 1051
AlwI GGATC 6 cut(s) 140, 364, 623, 629, 864, 926
AoxI GGCC 4 cut(s) 6, 102, 203, 569
ApeKI GCWGC 1 cut(s) 608
ApoI RAATTY 1 cut(s) 67
ArsI GACNNNNNNTTYG 2 cut(s) 229, 261
AsiGI ACCGGT 1 cut(s) 489
Asp700I GAANNNNTTC 1 cut(s) 97
Asp718I GGTACC 1 cut(s) 466
AspLEI GCGC 1 cut(s) 1046
AspS9I GGNCC 4 cut(s) 7, 103, 569, 1142
AsuHPI GGTGA 1 cut(s) 551
AvaII GGWCC 1 cut(s) 1142
AxyI CCTNAGG 1 cut(s) 517
BaeI ACNNNNGTAYC 2 cut(s) 280, 313
BalI TGGCCA 1 cut(s) 205
BanI GGYRCC 2 cut(s) 60, 466
BanII GRGCYC 1 cut(s) 696
BbsI GAAGAC 1 cut(s) 735
BbvI GCAGC 1 cut(s) 595
BciT130I CCWGG 4 cut(s) 345, 471, 793, 1041
BcuI ACTAGT 1 cut(s) 959
BfaI CTAG 1 cut(s) 960
BfmI CTRYAG 1 cut(s) 609
BfoI RGCGCY 1 cut(s) 1047
BfuAI ACCTGC 2 cut(s) 319, 907
BglII AGATCT 1 cut(s) 54
BisI GCNGC 1 cut(s) 609
BlpI GCTNAGC 1 cut(s) 1047
BlsI GCNGC 1 cut(s) 610
Bme1390I CCNGG 4 cut(s) 345, 471, 793, 1041
Bme18I GGWCC 1 cut(s) 1142
BmeRI GACNNNNNGTC 1 cut(s) 275
BmgT120I GGNCC 4 cut(s) 7, 103, 569, 1142
BmiI GGNNCC 4 cut(s) 62, 468, 789, 1144
BmrFI CCNGG 4 cut(s) 345, 471, 793, 1041
BmsI GCATC 6 cut(s) 157, 230, 340, 487, 902, 1082
BpiI GAAGAC 1 cut(s) 735
Bpu10I CCTNAGC 1 cut(s) 783
Bpu1102I GCTNAGC 1 cut(s) 1047
Bsa29I ATCGAT 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 633
BsaJI CCNNGG 5 cut(s) 85, 409, 791, 1006, 1108
BsaWI WCCGGW 1 cut(s) 489
Bsc4I CCNNNNNNNGG 3 cut(s) 317, 566, 1109
Bse118I RCCGGY 1 cut(s) 489
Bse1I ACTGG 3 cut(s) 445, 1022, 1098
Bse21I CCTNAGG 1 cut(s) 517
Bse8I GATNNNNATC 1 cut(s) 633
BseBI CCWGG 4 cut(s) 345, 471, 793, 1041
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 5 cut(s) 85, 409, 791, 1006, 1108
BseGI GGATG 2 cut(s) 172, 667
BseJI GATNNNNATC 1 cut(s) 633
BseLI CCNNNNNNNGG 3 cut(s) 317, 566, 1109
BseMII CTCAG 4 cut(s) 216, 531, 797, 1061
BseNI ACTGG 3 cut(s) 445, 1022, 1098
BseXI GCAGC 1 cut(s) 595
BshFI GGCC 4 cut(s) 8, 104, 205, 571
BshNI GGYRCC 2 cut(s) 60, 466
BshTI ACCGGT 1 cut(s) 489
BshVI ATCGAT 1 cut(s) 144
BsiSI CCGG 1 cut(s) 490
BslFI GGGAC 2 cut(s) 62, 1128
BslI CCNNNNNNNGG 3 cut(s) 317, 566, 1109
BsmFI GGGAC 2 cut(s) 62, 1128
BsmI GAATGC 2 cut(s) 377, 591
BsnI GGCC 4 cut(s) 8, 104, 205, 571
Bsp1286I GDGCHC 1 cut(s) 696
Bsp1407I TGTACA 1 cut(s) 1023
Bsp1720I GCTNAGC 1 cut(s) 1047
Bsp19I CCATGG 2 cut(s) 85, 1006
BspACI CCGC 2 cut(s) 46, 219
BspANI GGCC 4 cut(s) 8, 104, 205, 571
BspCNI CTCAG 4 cut(s) 217, 530, 796, 1060
BspDI ATCGAT 1 cut(s) 144
BspLI GGNNCC 4 cut(s) 62, 468, 789, 1144
BspMAI CTGCAG 1 cut(s) 613
BspMI ACCTGC 2 cut(s) 319, 907
BspPI GGATC 6 cut(s) 140, 364, 623, 629, 864, 926
BspT107I GGYRCC 2 cut(s) 60, 466
BsrFI RCCGGY 1 cut(s) 489
BsrGI TGTACA 1 cut(s) 1023
BsrI ACTGG 3 cut(s) 445, 1022, 1098
BssAI RCCGGY 1 cut(s) 489
BssECI CCNNGG 5 cut(s) 85, 409, 791, 1006, 1108
BssT1I CCWWGG 3 cut(s) 85, 1006, 1108
Bst2UI CCWGG 4 cut(s) 345, 471, 793, 1041
Bst4CI ACNGT 3 cut(s) 410, 866, 1134
BstAPI GCANNNNNTGC 2 cut(s) 320, 905
BstAUI TGTACA 1 cut(s) 1023
BstC8I GCNNGC 1 cut(s) 203
BstDEI CTNAG 6 cut(s) 31, 225, 517, 783, 1047, 1170
BstDSI CCRYGG 3 cut(s) 85, 409, 1006
BstENI CCTNNNNNAGG 1 cut(s) 1107
BstF5I GGATG 2 cut(s) 172, 667
BstH2I RGCGCY 1 cut(s) 1047
BstHHI GCGC 1 cut(s) 1046
BstMWI GCNNNNNNNGC 3 cut(s) 320, 691, 905
BstNI CCWGG 4 cut(s) 345, 471, 793, 1041
BstSCI CCNGG 4 cut(s) 343, 469, 791, 1039
BstSFI CTRYAG 1 cut(s) 609
BstV1I GCAGC 1 cut(s) 595
BstV2I GAAGAC 1 cut(s) 735
BstX2I RGATCY 1 cut(s) 54
BstXI CCANNNNNNTGG 2 cut(s) 242, 579
BstYI RGATCY 1 cut(s) 54
Bsu15I ATCGAT 1 cut(s) 144
Bsu36I CCTNAGG 1 cut(s) 517
BsuRI GGCC 4 cut(s) 8, 104, 205, 571
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 3 cut(s) 85, 409, 1006
BtsCI GGATG 2 cut(s) 172, 667
BveI ACCTGC 2 cut(s) 319, 907
Cac8I GCNNGC 1 cut(s) 203
CfoI GCGC 1 cut(s) 1046
Cfr10I RCCGGY 1 cut(s) 489
Cfr13I GGNCC 4 cut(s) 7, 103, 569, 1142
ClaI ATCGAT 1 cut(s) 144
CsiI ACCWGGT 1 cut(s) 469
Csp6I GTAC 7 cut(s) 194, 289, 438, 467, 1024, 1037, 1120
CspAI ACCGGT 1 cut(s) 489
CviAII CATG 8 cut(s) 86, 360, 462, 566, 812, 825, 998, 1007
CviQI GTAC 7 cut(s) 194, 289, 438, 467, 1024, 1037, 1120
DdeI CTNAG 6 cut(s) 31, 225, 517, 783, 1047, 1170
DriI GACNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 203
Eam1105I GACNNNNNGTC 1 cut(s) 275
Eco130I CCWWGG 3 cut(s) 85, 1006, 1108
Eco24I GRGCYC 1 cut(s) 696
Eco47I GGWCC 1 cut(s) 1142
Eco81I CCTNAGG 1 cut(s) 517
EcoNI CCTNNNNNAGG 1 cut(s) 1107
EcoO109I RGGNCCY 1 cut(s) 1142
EcoRII CCWGG 4 cut(s) 343, 469, 791, 1039
EcoT14I CCWWGG 3 cut(s) 85, 1006, 1108
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 1 cut(s) 696
ErhI CCWWGG 3 cut(s) 85, 1006, 1108
FaeI CATG 8 cut(s) 89, 363, 465, 569, 815, 828, 1001, 1010
FaqI GGGAC 2 cut(s) 62, 1128
FatI CATG 8 cut(s) 85, 359, 461, 565, 811, 824, 997, 1006
FauI CCCGC 2 cut(s) 39, 226
FblI GTMKAC 1 cut(s) 268
Fnu4HI GCNGC 1 cut(s) 609
FokI GGATG 2 cut(s) 179, 654
FriOI GRGCYC 1 cut(s) 696
Fsp4HI GCNGC 1 cut(s) 609
FspBI CTAG 1 cut(s) 960
GlaI GCGC 1 cut(s) 1045
GluI GCNGC 1 cut(s) 609
HaeII RGCGCY 1 cut(s) 1047
HaeIII GGCC 4 cut(s) 8, 104, 205, 571
HapII CCGG 1 cut(s) 490
HhaI GCGC 1 cut(s) 1046
Hin1II CATG 8 cut(s) 89, 363, 465, 569, 815, 828, 1001, 1010
Hin6I GCGC 1 cut(s) 1044
HinP1I GCGC 1 cut(s) 1044
HincII GTYRAC 1 cut(s) 248
HindII GTYRAC 1 cut(s) 248
HinfI GANTC 2 cut(s) 938, 1002
HpaII CCGG 1 cut(s) 490
HphI GGTGA 1 cut(s) 551
Hpy166II GTNNAC 3 cut(s) 248, 269, 739
Hpy188I TCNGA 3 cut(s) 226, 861, 924
Hpy188III TCNNGA 8 cut(s) 391, 596, 626, 632, 671, 697, 722, 935
Hpy8I GTNNAC 3 cut(s) 248, 269, 739
HpyAV CCTTC 3 cut(s) 87, 809, 1133
HpyCH4III ACNGT 3 cut(s) 410, 866, 1134
HpyCH4IV ACGT 1 cut(s) 1035
HpyF10VI GCNNNNNNNGC 3 cut(s) 320, 691, 905
HpyF3I CTNAG 6 cut(s) 31, 225, 517, 783, 1047, 1170
HpySE526I ACGT 1 cut(s) 1035
Hsp92II CATG 8 cut(s) 89, 363, 465, 569, 815, 828, 1001, 1010
HspAI GCGC 1 cut(s) 1044
KpnI GGTACC 1 cut(s) 470
Lsp1109I GCAGC 1 cut(s) 595
LweI GCATC 6 cut(s) 157, 230, 340, 487, 902, 1082
MabI ACCWGGT 1 cut(s) 469
MaeI CTAG 1 cut(s) 960
MaeII ACGT 1 cut(s) 1035
MboII GAAGA 5 cut(s) 102, 119, 311, 740, 1066
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 1 cut(s) 54
MhlI GDGCHC 1 cut(s) 696
MlsI TGGCCA 1 cut(s) 205
MluCI AATT 8 cut(s) 67, 123, 134, 386, 713, 875, 888, 911
MluNI TGGCCA 1 cut(s) 205
MnlI CCTC 7 cut(s) 157, 220, 526, 567, 960, 969, 1051
Mox20I TGGCCA 1 cut(s) 205
Mph1103I ATGCAT 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 97
MscI TGGCCA 1 cut(s) 205
MseI TTAA 5 cut(s) 137, 426, 507, 927, 1032
Msp20I TGGCCA 1 cut(s) 205
MspI CCGG 1 cut(s) 490
MspR9I CCNGG 4 cut(s) 345, 471, 793, 1041
MunI CAATTG 1 cut(s) 888
Mva1269I GAATGC 2 cut(s) 377, 591
MvaI CCWGG 4 cut(s) 345, 471, 793, 1041
MwoI GCNNNNNNNGC 3 cut(s) 320, 691, 905
NcoI CCATGG 2 cut(s) 85, 1006
NlaIII CATG 8 cut(s) 89, 363, 465, 569, 815, 828, 1001, 1010
NlaIV GGNNCC 4 cut(s) 62, 468, 789, 1144
NsiI ATGCAT 1 cut(s) 463
PaqCI CACCTGC 1 cut(s) 319
PctI GAATGC 2 cut(s) 377, 591
PdmI GAANNNNTTC 1 cut(s) 97
PfeI GAWTC 2 cut(s) 938, 1002
PflMI CCANNNNNTGG 1 cut(s) 566
PinAI ACCGGT 1 cut(s) 489
PkrI GCNGC 1 cut(s) 610
PpuMI RGGWCCY 1 cut(s) 1142
Psp5II RGGWCCY 1 cut(s) 1142
Psp6I CCWGG 4 cut(s) 343, 469, 791, 1039
PspGI CCWGG 4 cut(s) 343, 469, 791, 1039
PspN4I GGNNCC 4 cut(s) 62, 468, 789, 1144
PspPI GGNCC 4 cut(s) 7, 103, 569, 1142
PspPPI RGGWCCY 1 cut(s) 1142
PsrI GAACNNNNNNTAC 1 cut(s) 1147
PstI CTGCAG 1 cut(s) 613
PsuI RGATCY 1 cut(s) 54
RsaI GTAC 7 cut(s) 195, 290, 439, 468, 1025, 1038, 1121
RsaNI GTAC 7 cut(s) 194, 289, 438, 467, 1024, 1037, 1120
SaqAI TTAA 5 cut(s) 137, 426, 507, 927, 1032
SatI GCNGC 1 cut(s) 609
Sau96I GGNCC 4 cut(s) 7, 103, 569, 1142
ScrFI CCNGG 4 cut(s) 345, 471, 793, 1041
SduI GDGCHC 1 cut(s) 696
SexAI ACCWGGT 1 cut(s) 469
SfaNI GCATC 6 cut(s) 157, 230, 340, 487, 902, 1082
SfcI CTRYAG 1 cut(s) 609
SinI GGWCC 1 cut(s) 1142
SpeI ACTAGT 1 cut(s) 959
Sse9I AATT 8 cut(s) 67, 123, 134, 386, 713, 875, 888, 911
SsiI CCGC 2 cut(s) 46, 219
SspMI CTAG 1 cut(s) 960
StyD4I CCNGG 4 cut(s) 343, 469, 791, 1039
StyI CCWWGG 3 cut(s) 85, 1006, 1108
TaaI ACNGT 3 cut(s) 410, 866, 1134
TaiI ACGT 1 cut(s) 1038
TaqI TCGA 2 cut(s) 144, 531
TasI AATT 8 cut(s) 67, 123, 134, 386, 713, 875, 888, 911
TatI WGTACW 2 cut(s) 193, 1023
TfiI GAWTC 2 cut(s) 938, 1002
Tru1I TTAA 5 cut(s) 137, 426, 507, 927, 1032
Tru9I TTAA 5 cut(s) 137, 426, 507, 927, 1032
TseI GCWGC 1 cut(s) 608
TspDTI ATGAA 5 cut(s) 567, 649, 741, 828, 1155
TspGWI ACGGA 2 cut(s) 138, 822
Van91I CCANNNNNTGG 1 cut(s) 566
VpaK11BI GGWCC 1 cut(s) 1142
XagI CCTNNNNNAGG 1 cut(s) 1107
XapI RAATTY 1 cut(s) 67
XmiI GTMKAC 1 cut(s) 268
XmnI GAANNNNTTC 1 cut(s) 97
XspI CTAG 1 cut(s) 960
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.