MD08G1227800.v1.1

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003186
Physical Location & Seq
Forward (+)
29406791 .. 29416525
9735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1227800.v1.1.491

Sequence Viewer

Length: 2490 bp
ATGCTGTCCAAAATCTCAGTTACCCAGTTGAAAACATCACCTGCTGTACCCGTTTCTGGATTTTCATGGCGTGGGAATCGTCCTAGGCTTGGATATTGTGCCGGGAATTCTTCAGAGCGTAACAAGTTGCGGTTCAGGATTGTGGGGCAGTCTCTGGGGGATAGGTGGAAGCTCAACGACGTTGATGCAAATGCCGTACAAGAAAAGTTGAATTCATGGCTAGTAAAGACGCAAAGCTTTTTAAATGAAGTGGCGTCCACGTCTCCATTGGCAAGAACTGGTCAGACTAAAAAGCCTGTCGCTACAGATGCTTCAGAGACACAAGATATGGAGGACATATTTATGGCAGAACAGACATTTAATAGCAGAACACCAAATGGAATTCTCTCTTTAGCTGCCGTTGTATCTATAGAGCAATTTAGCAGGATGAATGGATTGACGGGGCAGAAGATGCAGAAAATATTTAAAGCACTTGTTCCTGAATCTACATACAATGATGCTCGCAATTTGGTAGAATATTGCTGCTTCAGGTTTCTGTCAAGGGATAATTCTGATATTCATCCTTCCCTCAAGGAACCTTCATTCCAAAGACTTATATTTATAACTATGCTTGCATGGGAGAATCCTTATCGAGAAGATCTTGCTAATGGTTCCGAGAAAGCTTCCTTTCAGAGAAAGCTTGTCAGAGAAGAGGCTTTTGTTCGAATGGCTCCTGCTATTTCTGGTGTGGCTGATAGGTCAACAGCGCATAATCTATTTAAGGCTCTTGCCGGTGATGAACAGGGCATCTCTCTGAGCACGTGGTTGACTTATGTTGATGAACTTCTCAAGATTCACGAAGGAAGAAAATCATACCAGACTAGACAAAGTTCCAACCTTCCTGAGGAGAGAATTTTATGCATTGGTTCCAGCAGGAAGCGGCCTGTCCTAAAATGGGAGAACAACATGGCATGGCCAGGAAAAGTAACATTAACTGATAAAGCAATTTATTTTGAGGCCGTTGGCCTTGCTGGGCAAAAGGATTCTATAAGATTGGATCTTACAAAACAAGGATTACAGATTAAGAAAGCAAAAGTTGGACCTTTTGGTTCTGGTCTCTTCGACTCTGCCGTCTCTATATCGTATGGTCCCGTATCAGAAACATGGGTGCTTGAATTTGTGGACTTAGGTGGAGAAATGAGGCGAGACGTGTGGCATGCATTTATTAGAGAAATTAATGCTGTTCACAAGTTTATCCGTGATTATGGACCGGAGGAATTTGATGAATCCATATTTCATGTTTATGGTGCTCACAAAGGGAAGGAAAGAGCTATGGCCACTGCCATTAATAGTATAGCTAGACTACAGGCTCTTCAATTTATGGGGAAGTTGCTGGATGATCCCACCAAACATGTTCAGTTTACATATTTACAGTATGCACCATTTGGTGATGTAGTTTCACAGACTCTAGCAGTAAATTATTGGGGTGGACCACTGATATCAAAGTATTTAGAGATGGACAACCAGCCAGCTCAAGGAGCGAGGGCTTCTAATGAAATGATTGAAAGCAGCAGCAATCATGTGTTTGACATAGATGGCAGTGTTTACTTGCACAAATGGAAGAGATCTCCATGTTGGGCTTCAAGTGCTTCTGTTGCCTTTTGGAAGAACACTTCAACAAGACAGGGGCTAGTTCTAAGTAAAAATCTGGTTGTTGCTGATGTGTCCCTTGTTGAAAGGGCAACAAGAACATGCAAACAAAAATGGCAGGAAGCTGAAACAACTCAAGCCACAATTGATGCTGCAACGCTTAAGGGAATACCAAGTAACATTGATCTATTCAAGGAACTTCTGTTTCCCCTGACCATAACTGTGAGAAATTTTGAAAAACTTAGGCGCTGGGAGGAGCCATATTTGACCATTTCTTTTCTTGCCTTTGCATATACAGTCATTTTCAGGAATTTGCTGTCATATGTATTCCCAGCGGCATTGATAATTTTGGCAGCTGTCATGCTGACATTGAAGGGGCTAAAGGAGCAAGGCCGCCTTGGACGATCATTTGGAAAAATTACCATCCGTGACCAGCCTCCTTCAAATACCATTGAGAAAATTATAGCCGTAAAAGATGCCATGCGCGATGTGGAAAGTTATTTACAGAATCTGAATGTCACGCTTCTGAAAATACATACAGTTTTCCTTTCTGGTCAGCCTCAGATAACGACTGAGGTTGCGCTGGTGTTGTTATCTTCTGCAACCATTCTCCTCATTTTCCCCTTCAAATATGTTCTTGCCTTTTTTATCTTCGATCTGTTCACACGGGAGCTTGAGTTCAGGAGGGAAATGGTTAAAAGGTTTATGAAACTCTTGAAGGAGCGTTGGGACAGAGTGCCTGCAGCCCCTGTAGTTGTTTTGCCTTTTGGAAGTGAAGAGCCAACACCAAAACCGAATACAAAGGCAAGCAAGGATCCAGAAAGGTCAGAAAGAAGCCTTGGCAGCAGCAATTCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

830

Amino Acids

93.5

Weight (kDa)

9.32

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 568 - 799 1e-84 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 602
AarI CACCTGC 1 cut(s) 49
AasI GACNNNNNNGTC 1 cut(s) 1109
Acc36I ACCTGC 1 cut(s) 49
AccII CGCG 1 cut(s) 2115
AciI CCGC 4 cut(s) 130, 919, 1964, 2023
AclWI GGATC 4 cut(s) 1044, 1373, 2438, 2451
AcoI YGGCCR 2 cut(s) 953, 1314
AcsI RAATTY 8 cut(s) 106, 211, 381, 891, 1154, 1256, 1858, 1939
AcuI CTGAAG 3 cut(s) 96, 297, 511
AcvI CACGTG 1 cut(s) 801
AcyI GRCGYC 1 cut(s) 254
AfaI GTAC 2 cut(s) 48, 198
AfiI CCNNNNNNNGG 5 cut(s) 56, 89, 883, 934, 1514
AflII CTTAAG 1 cut(s) 1790
AflIII ACRYGT 2 cut(s) 1188, 1390
AjiI CACGTC 2 cut(s) 261, 1189
AjnI CCWGG 1 cut(s) 955
AjuI GAANNNNNNNTTGG 2 cut(s) 2451, 2483
AloI GAACNNNNNNTCC 4 cut(s) 567, 599, 2291, 2323
Alw21I GWGCWC 2 cut(s) 800, 1291
Alw26I GTCTC 6 cut(s) 156, 267, 311, 1100, 1117, 1179
AlwI GGATC 4 cut(s) 1044, 1373, 2438, 2451
AlwNI CAGNNNCTG 3 cut(s) 154, 2140, 2378
AoxI GGCC 6 cut(s) 920, 953, 996, 1003, 1314, 2020
ApeKI GCWGC 9 cut(s) 395, 522, 1548, 1551, 1781, 1982, 2372, 2472, 2475
ApoI RAATTY 8 cut(s) 106, 211, 381, 891, 1154, 1256, 1858, 1939
AseI ATTAAT 2 cut(s) 1215, 1326
AspA2I CCTAGG 1 cut(s) 83
AspLEI GCGC 4 cut(s) 748, 1878, 2115, 2212
AspS9I GGNCC 4 cut(s) 1079, 1127, 1247, 1469
AsuC2I CCSGG 1 cut(s) 103
AsuHPI GGTGA 3 cut(s) 30, 785, 1439
AsuII TTCGAA 1 cut(s) 703
AvaII GGWCC 4 cut(s) 1079, 1127, 1247, 1469
AvrII CCTAGG 1 cut(s) 83
AxyI CCTNAGG 1 cut(s) 882
BalI TGGCCA 2 cut(s) 955, 1316
BamHI GGATCC 1 cut(s) 2443
BbrPI CACGTG 1 cut(s) 801
Bbv12I GWGCWC 2 cut(s) 800, 1291
BbvI GCAGC 8 cut(s) 382, 509, 1560, 1563, 1768, 1994, 2384, 2484
BccI CCATC 3 cut(s) 1489, 1568, 2060
BceAI ACGGC 5 cut(s) 179, 383, 983, 1094, 2081
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BciT130I CCWGG 1 cut(s) 957
BcnI CCSGG 1 cut(s) 103
BcoDI GTCTC 6 cut(s) 156, 267, 311, 1100, 1117, 1179
BfaI CTAG 6 cut(s) 84, 221, 861, 1338, 1448, 1670
BfmI CTRYAG 5 cut(s) 303, 408, 1343, 2370, 2379
BfoI RGCGCY 1 cut(s) 1879
BfrI CTTAAG 1 cut(s) 1790
BfuAI ACCTGC 1 cut(s) 49
BglII AGATCT 2 cut(s) 637, 1604
BlnI CCTAGG 1 cut(s) 83
Bme1390I CCNGG 2 cut(s) 103, 957
Bme18I GGWCC 4 cut(s) 1079, 1127, 1247, 1469
BmgBI CACGTC 2 cut(s) 261, 1189
BmgT120I GGNCC 4 cut(s) 1079, 1127, 1247, 1469
BmiI GGNNCC 7 cut(s) 576, 652, 711, 907, 1129, 1887, 2445
BmrFI CCNGG 2 cut(s) 103, 957
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 7 cut(s) 175, 298, 441, 487, 795, 1768, 2095
BmuI ACTGGG 1 cut(s) 19
Bpu14I TTCGAA 1 cut(s) 703
BpuEI CTTGAG 5 cut(s) 554, 812, 1497, 1749, 2324
BpuMI CCSGG 1 cut(s) 103
BsaAI YACGTR 1 cut(s) 801
BsaBI GATNNNNATC 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 254
BsaI GGTCTC 1 cut(s) 1100
BsaJI CCNNGG 3 cut(s) 83, 2026, 2467
BsaWI WCCGGW 1 cut(s) 1249
BsaXI ACNNNNNCTCC 2 cut(s) 2291, 2321
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 89, 883, 934, 1514
Bse118I RCCGGY 1 cut(s) 770
Bse1I ACTGG 2 cut(s) 25, 283
Bse21I CCTNAGG 1 cut(s) 882
Bse8I GATNNNNATC 1 cut(s) 558
BseBI CCWGG 1 cut(s) 957
BseDI CCNNGG 3 cut(s) 83, 2026, 2467
BseGI GGATG 4 cut(s) 432, 559, 1381, 2052
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 5 cut(s) 56, 89, 883, 934, 1514
BseMII CTCAG 5 cut(s) 30, 785, 873, 2193, 2204
BseNI ACTGG 2 cut(s) 25, 283
BseRI GAGGAG 3 cut(s) 899, 1898, 2231
BseXI GCAGC 8 cut(s) 382, 509, 1560, 1563, 1768, 1994, 2384, 2484
BseYI CCCAGC 3 cut(s) 1010, 1878, 1960
Bsh1236I CGCG 1 cut(s) 2115
BshFI GGCC 6 cut(s) 922, 955, 998, 1005, 1316, 2022
BsiHKAI GWGCWC 2 cut(s) 800, 1291
BsiSI CCGG 3 cut(s) 102, 771, 1250
BslFI GGGAC 3 cut(s) 1113, 1690, 2372
BslI CCNNNNNNNGG 5 cut(s) 56, 89, 883, 934, 1514
BsmAI GTCTC 6 cut(s) 156, 267, 311, 1100, 1117, 1179
BsmBI CGTCTC 3 cut(s) 267, 1117, 1179
BsmFI GGGAC 3 cut(s) 1113, 1690, 2372
BsnI GGCC 6 cut(s) 922, 955, 998, 1005, 1316, 2022
Bso31I GGTCTC 1 cut(s) 1100
Bsp119I TTCGAA 1 cut(s) 703
Bsp1286I GDGCHC 2 cut(s) 800, 1291
Bsp143I GATC 8 cut(s) 637, 1036, 1378, 1604, 1813, 2033, 2284, 2443
BspACI CCGC 4 cut(s) 130, 919, 1964, 2023
BspANI GGCC 6 cut(s) 922, 955, 998, 1005, 1316, 2022
BspCNI CTCAG 5 cut(s) 29, 786, 874, 2194, 2203
BspFNI CGCG 1 cut(s) 2115
BspLI GGNNCC 7 cut(s) 576, 652, 711, 907, 1129, 1887, 2445
BspMAI CTGCAG 1 cut(s) 2374
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 4 cut(s) 1044, 1373, 2438, 2451
BspQI GCTCTTC 2 cut(s) 1356, 2400
BspT104I TTCGAA 1 cut(s) 703
BspTI CTTAAG 1 cut(s) 1790
BspTNI GGTCTC 1 cut(s) 1100
BsrFI RCCGGY 1 cut(s) 770
BsrI ACTGG 2 cut(s) 25, 283
BssAI RCCGGY 1 cut(s) 770
BssECI CCNNGG 3 cut(s) 83, 2026, 2467
BssMI GATC 8 cut(s) 637, 1036, 1378, 1604, 1813, 2033, 2284, 2443
BssNI GRCGYC 1 cut(s) 254
BssT1I CCWWGG 3 cut(s) 83, 2026, 2467
Bst2UI CCWGG 1 cut(s) 957
Bst4CI ACNGT 4 cut(s) 1413, 1852, 1927, 2170
Bst6I CTCTTC 5 cut(s) 684, 1103, 1356, 1595, 2400
BstACI GRCGYC 1 cut(s) 254
BstAFI CTTAAG 1 cut(s) 1790
BstAPI GCANNNNNTGC 1 cut(s) 451
BstBAI YACGTR 1 cut(s) 801
BstBI TTCGAA 1 cut(s) 703
BstC8I GCNNGC 6 cut(s) 502, 612, 1197, 1509, 2370, 2437
BstDEI CTNAG 8 cut(s) 16, 794, 882, 1165, 1676, 1871, 2190, 2202
BstENI CCTNNNNNAGG 1 cut(s) 881
BstF5I GGATG 4 cut(s) 432, 559, 1381, 2052
BstFNI CGCG 1 cut(s) 2115
BstH2I RGCGCY 1 cut(s) 1879
BstHHI GCGC 4 cut(s) 748, 1878, 2115, 2212
BstKTI GATC 8 cut(s) 640, 1039, 1381, 1607, 1816, 2036, 2287, 2446
BstMAI GTCTC 6 cut(s) 156, 267, 311, 1100, 1117, 1179
BstMBI GATC 8 cut(s) 637, 1036, 1378, 1604, 1813, 2033, 2284, 2443
BstMWI GCNNNNNNNGC 7 cut(s) 308, 451, 1517, 1625, 1634, 2014, 2472
BstNI CCWGG 1 cut(s) 957
BstNSI RCATGY 3 cut(s) 1199, 1394, 1734
BstSCI CCNGG 2 cut(s) 101, 955
BstSFI CTRYAG 5 cut(s) 303, 408, 1343, 2370, 2379
BstUI CGCG 1 cut(s) 2115
BstV1I GCAGC 8 cut(s) 382, 509, 1560, 1563, 1768, 1994, 2384, 2484
BstX2I RGATCY 4 cut(s) 637, 1036, 1604, 2443
BstYI RGATCY 4 cut(s) 637, 1036, 1604, 2443
Bsu36I CCTNAGG 1 cut(s) 882
BsuRI GGCC 6 cut(s) 922, 955, 998, 1005, 1316, 2022
BtgZI GCGATG 1 cut(s) 2130
BtrI CACGTC 2 cut(s) 261, 1189
BtsCI GGATG 4 cut(s) 432, 559, 1381, 2052
BtsI GCAGTG 2 cut(s) 1317, 1585
BtsIMutI CAGTG 3 cut(s) 1317, 1472, 1585
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 6 cut(s) 502, 612, 1197, 1509, 2370, 2437
CaiI CAGNNNCTG 3 cut(s) 154, 2140, 2378
CfoI GCGC 4 cut(s) 748, 1878, 2115, 2212
Cfr10I RCCGGY 1 cut(s) 770
Cfr13I GGNCC 4 cut(s) 1079, 1127, 1247, 1469
CseI GACGC 2 cut(s) 238, 243
Csp6I GTAC 2 cut(s) 47, 197
CviQI GTAC 2 cut(s) 47, 197
DdeI CTNAG 8 cut(s) 16, 794, 882, 1165, 1676, 1871, 2190, 2202
DpnI GATC 8 cut(s) 639, 1038, 1380, 1606, 1815, 2035, 2286, 2445
DpnII GATC 8 cut(s) 637, 1036, 1378, 1604, 1813, 2033, 2284, 2443
DraI TTTAAA 2 cut(s) 243, 466
DrdI GACNNNNNNGTC 1 cut(s) 1109
DseDI GACNNNNNNGTC 1 cut(s) 1109
EaeI YGGCCR 2 cut(s) 953, 1314
Eam1104I CTCTTC 5 cut(s) 684, 1103, 1356, 1595, 2400
EarI CTCTTC 5 cut(s) 684, 1103, 1356, 1595, 2400
Eco130I CCWWGG 3 cut(s) 83, 2026, 2467
Eco31I GGTCTC 1 cut(s) 1100
Eco32I GATATC 1 cut(s) 1479
Eco47I GGWCC 4 cut(s) 1079, 1127, 1247, 1469
Eco57I CTGAAG 3 cut(s) 96, 297, 511
Eco72I CACGTG 1 cut(s) 801
Eco81I CCTNAGG 1 cut(s) 882
EcoNI CCTNNNNNAGG 1 cut(s) 881
EcoRI GAATTC 3 cut(s) 106, 211, 381
EcoRII CCWGG 1 cut(s) 955
EcoRV GATATC 1 cut(s) 1479
EcoT14I CCWWGG 3 cut(s) 83, 2026, 2467
EcoT22I ATGCAT 2 cut(s) 902, 1201
ErhI CCWWGG 3 cut(s) 83, 2026, 2467
Esp3I CGTCTC 3 cut(s) 267, 1117, 1179
FalI AAGNNNNNCTT 2 cut(s) 2010, 2042
FaqI GGGAC 3 cut(s) 1113, 1690, 2372
FauNDI CATATG 1 cut(s) 1951
FokI GGATG 4 cut(s) 439, 546, 1388, 2039
FspBI CTAG 6 cut(s) 84, 221, 861, 1338, 1448, 1670
GlaI GCGC 4 cut(s) 747, 1877, 2114, 2211
GsaI CCCAGC 3 cut(s) 1014, 1882, 1964
HaeII RGCGCY 1 cut(s) 1879
HaeIII GGCC 6 cut(s) 922, 955, 998, 1005, 1316, 2022
HapII CCGG 3 cut(s) 102, 771, 1250
HgaI GACGC 2 cut(s) 238, 243
HhaI GCGC 4 cut(s) 748, 1878, 2115, 2212
Hin1I GRCGYC 1 cut(s) 254
Hin6I GCGC 4 cut(s) 746, 1876, 2113, 2210
HinP1I GCGC 4 cut(s) 746, 1876, 2113, 2210
HincII GTYRAC 2 cut(s) 741, 807
HindII GTYRAC 2 cut(s) 741, 807
HindIII AAGCTT 3 cut(s) 235, 660, 677
HinfI GANTC 9 cut(s) 76, 482, 622, 832, 1022, 1103, 1265, 1444, 2137
HpaII CCGG 3 cut(s) 102, 771, 1250
HphI GGTGA 3 cut(s) 30, 785, 1439
Hpy166II GTNNAC 9 cut(s) 258, 741, 807, 1162, 1225, 1401, 1469, 1585, 2292
Hpy8I GTNNAC 9 cut(s) 258, 741, 807, 1162, 1225, 1401, 1469, 1585, 2292
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 9 cut(s) 573, 588, 833, 887, 1294, 1996, 2079, 2263, 2341
HpyCH4III ACNGT 4 cut(s) 1413, 1852, 1927, 2170
HpyCH4IV ACGT 4 cut(s) 180, 260, 800, 1188
HpyF10VI GCNNNNNNNGC 7 cut(s) 308, 451, 1517, 1625, 1634, 2014, 2472
HpyF3I CTNAG 8 cut(s) 16, 794, 882, 1165, 1676, 1871, 2190, 2202
HpySE526I ACGT 4 cut(s) 180, 260, 800, 1188
Hsp92I GRCGYC 1 cut(s) 254
HspAI GCGC 4 cut(s) 746, 1876, 2113, 2210
Kzo9I GATC 8 cut(s) 637, 1036, 1378, 1604, 1813, 2033, 2284, 2443
LguI GCTCTTC 2 cut(s) 1356, 2400
LmnI GCTCC 6 cut(s) 715, 1517, 1885, 2014, 2299, 2350
Lsp1109I GCAGC 8 cut(s) 382, 509, 1560, 1563, 1768, 1994, 2384, 2484
LweI GCATC 7 cut(s) 175, 298, 441, 487, 795, 1768, 2095
MaeI CTAG 6 cut(s) 84, 221, 861, 1338, 1448, 1670
MaeII ACGT 4 cut(s) 180, 260, 800, 1188
MaeIII GTNAC 6 cut(s) 19, 119, 964, 1805, 2057, 2146
MalI GATC 8 cut(s) 639, 1038, 1380, 1606, 1815, 2035, 2286, 2445
MboI GATC 8 cut(s) 637, 1036, 1378, 1604, 1813, 2033, 2284, 2443
MfeI CAATTG 1 cut(s) 1773
MflI RGATCY 4 cut(s) 637, 1036, 1604, 2443
MhlI GDGCHC 2 cut(s) 800, 1291
MlsI TGGCCA 2 cut(s) 955, 1316
MluNI TGGCCA 2 cut(s) 955, 1316
MlyI GAGTC 2 cut(s) 1097, 1438
MmeI TCCRAC 2 cut(s) 897, 1057
Mox20I TGGCCA 2 cut(s) 955, 1316
Mph1103I ATGCAT 2 cut(s) 902, 1201
MscI TGGCCA 2 cut(s) 955, 1316
MslI CAYNNNNRTG 3 cut(s) 341, 1281, 1850
Msp20I TGGCCA 2 cut(s) 955, 1316
MspA1I CMGCKG 2 cut(s) 1964, 1985
MspCI CTTAAG 1 cut(s) 1790
MspI CCGG 3 cut(s) 102, 771, 1250
MspR9I CCNGG 2 cut(s) 103, 957
MunI CAATTG 1 cut(s) 1773
MvaI CCWGG 1 cut(s) 957
MvnI CGCG 1 cut(s) 2115
MwoI GCNNNNNNNGC 7 cut(s) 308, 451, 1517, 1625, 1634, 2014, 2472
NciI CCSGG 1 cut(s) 103
NdeI CATATG 1 cut(s) 1951
NdeII GATC 8 cut(s) 637, 1036, 1378, 1604, 1813, 2033, 2284, 2443
NlaIV GGNNCC 7 cut(s) 576, 652, 711, 907, 1129, 1887, 2445
NmuCI GTSAC 2 cut(s) 2057, 2146
NsiI ATGCAT 2 cut(s) 902, 1201
NspI RCATGY 3 cut(s) 1199, 1394, 1734
NspV TTCGAA 1 cut(s) 703
PaeI GCATGC 1 cut(s) 1199
PaqCI CACCTGC 1 cut(s) 49
PciI ACATGT 1 cut(s) 1390
PciSI GCTCTTC 2 cut(s) 1356, 2400
PcsI WCGNNNNNNNCGW 1 cut(s) 1107
PfeI GAWTC 7 cut(s) 76, 482, 622, 832, 1022, 1265, 2137
PleI GAGTC 2 cut(s) 1097, 1438
PmaCI CACGTG 1 cut(s) 801
PmlI CACGTG 1 cut(s) 801
PpsI GAGTC 2 cut(s) 1097, 1438
Ppu21I YACGTR 1 cut(s) 801
PscI ACATGT 1 cut(s) 1390
PshBI ATTAAT 2 cut(s) 1215, 1326
PsiI TTATAA 1 cut(s) 602
Psp6I CCWGG 1 cut(s) 955
PspCI CACGTG 1 cut(s) 801
PspFI CCCAGC 3 cut(s) 1010, 1878, 1960
PspGI CCWGG 1 cut(s) 955
PspN4I GGNNCC 7 cut(s) 576, 652, 711, 907, 1129, 1887, 2445
PspPI GGNCC 4 cut(s) 1079, 1127, 1247, 1469
PstI CTGCAG 1 cut(s) 2374
PstNI CAGNNNCTG 3 cut(s) 154, 2140, 2378
PsuI RGATCY 4 cut(s) 637, 1036, 1604, 2443
PvuII CAGCTG 1 cut(s) 1985
RsaI GTAC 2 cut(s) 48, 198
RsaNI GTAC 2 cut(s) 47, 197
RseI CAYNNNNRTG 3 cut(s) 341, 1281, 1850
SapI GCTCTTC 2 cut(s) 1356, 2400
Sau3AI GATC 8 cut(s) 637, 1036, 1378, 1604, 1813, 2033, 2284, 2443
Sau96I GGNCC 4 cut(s) 1079, 1127, 1247, 1469
SchI GAGTC 2 cut(s) 1097, 1438
ScrFI CCNGG 2 cut(s) 103, 957
SduI GDGCHC 2 cut(s) 800, 1291
SfaNI GCATC 7 cut(s) 175, 298, 441, 487, 795, 1768, 2095
SfcI CTRYAG 5 cut(s) 303, 408, 1343, 2370, 2379
SfuI TTCGAA 1 cut(s) 703
SinI GGWCC 4 cut(s) 1079, 1127, 1247, 1469
SmiMI CAYNNNNRTG 3 cut(s) 341, 1281, 1850
SmlI CTYRAG 6 cut(s) 569, 827, 1512, 1764, 1790, 2303
SmoI CTYRAG 6 cut(s) 569, 827, 1512, 1764, 1790, 2303
SphI GCATGC 1 cut(s) 1199
SsiI CCGC 4 cut(s) 130, 919, 1964, 2023
SspI AATATT 2 cut(s) 462, 518
SspMI CTAG 6 cut(s) 84, 221, 861, 1338, 1448, 1670
StyD4I CCNGG 2 cut(s) 101, 955
StyI CCWWGG 3 cut(s) 83, 2026, 2467
TaaI ACNGT 4 cut(s) 1413, 1852, 1927, 2170
TaiI ACGT 4 cut(s) 183, 263, 803, 1191
TaqI TCGA 4 cut(s) 631, 703, 1101, 2283
TauI GCSGC 3 cut(s) 922, 1967, 2025
TfiI GAWTC 7 cut(s) 76, 482, 622, 832, 1022, 1265, 2137
TscAI CASTG 3 cut(s) 1324, 1479, 1585
TseFI GTSAC 2 cut(s) 2057, 2146
TseI GCWGC 9 cut(s) 395, 522, 1548, 1551, 1781, 1982, 2372, 2472, 2475
Tsp45I GTSAC 2 cut(s) 2057, 2146
TspGWI ACGGA 2 cut(s) 1226, 2045
TspRI CASTG 3 cut(s) 1324, 1479, 1585
Vha464I CTTAAG 1 cut(s) 1790
VpaK11BI GGWCC 4 cut(s) 1079, 1127, 1247, 1469
VspI ATTAAT 2 cut(s) 1215, 1326
XagI CCTNNNNNAGG 1 cut(s) 881
XapI RAATTY 8 cut(s) 106, 211, 381, 891, 1154, 1256, 1858, 1939
XceI RCATGY 3 cut(s) 1199, 1394, 1734
XcmI CCANNNNNNNNNTGG 2 cut(s) 265, 2116
XmaJI CCTAGG 1 cut(s) 83
XspI CTAG 6 cut(s) 84, 221, 861, 1338, 1448, 1670
Zsp2I ATGCAT 2 cut(s) 902, 1201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.