Rh7AG356200

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0003186
Physical Location & Seq
Reverse (-)
45619135 .. 45632089
12955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG356200.1

Sequence Viewer

Length: 1806 bp
ATGAAACAAATGTCAAAACAGTCTCATTTTTTTTTTCCCCTGAACTCTTGTTTCATGATAGATGCGGTGCAAGAAAAGTTGAATTCATGGCTACTGAAGACCCAAAGCTTTTTAAGTGAAGTTACTTCTCCACTAGTAAGAACTAGTCAGACTGGAAAACCTGTCACCAGAGATGCTCTTGAGACACAAGATATGGACGACATATTTATGGCTGAACAGACTATTAATAGCAGAACACCAAATGGAATTCTCTCTTTGGCTGCTATTGTATCTGTAGAGCAGTTTAGCAGGATGAATGGATTGACGGGGCAGAAAATGCAGAAAATATTCAAAGCGCTTGTTCCTGAATCTACATATAATGATGCTCGTAATTTGGTTGAATATTGCTGCTTTAGGTTTTTGTCAAGGGATGCTTCTGATGTTCATCCTTCCCTGAAGGAACCTGCATTCCAAAGACTGATATTCATAACAATGCTTGCATGGGAGAATCCTTATCAAGAACATCTTGCCAGTGGTTCGGAGAAAGCTTCTTTTCAGAGAAAACTTGTGAGAGAAGATGCCTTTGTACGTTTGGCTCCTGCAATTTCTGGTGTGGCTGATAGGTCCACAGTGCATAATCTATTTAAGGCTCTAGCTGGTGATGAACAAGGCATCTCTTTGAGCTTGTGGTTGACTTATGTTGATGAACTTCTCAAGGTTCACGAAGGAAGAAAATCATATCAGATACGAGAGTCCCCGAACCTTTCTGAGGAGAGAATCTTATGCATTGGTTCCAGCAGGAAACGTCCTGTCTTAAAATGGGAGAAAAATATGGCCTGGCCTGGAAAAGTTACTCTAACTGATAAAGCTATTTATTTTGAGGCAGCTGGCTTATTTGGACAAAATGATTCTATGAAGTTGGATTTGACAAGACCTGGATTGCGAGTAGAAAAAGCAAAAGTTGGACCTTTTGGTTCTGTTCTCTTTGATTCTGCGGTCTCTATATCATATGGTCCCGAGTCAAAAGACACGTGGGTGCTGGAATTTGTTGACTTGGGTGGAGAAATGAGGCGAGATGTCTGGCATGCGTTTATTAGTGAGATTATTGTGTTACACAAGTTTATCAGGGAGTATGGTCCAAAGGAAGATGATGAATCGTTATTTCATGTTTATGGAGCTCACAAAGGAAAGGAAAGAGCTATCACCAGTGCCATTAATAGTATAGCTAGACTACAGGCTCTTCAGTTTATGAGGAAGTTGCTAGATGATCCGACCAAACTAGCTCAGTTCACATATCTACAATATGCACCATATGGTGATATTGTTTCTCAGGCTCTAGCAGTAAACTATTGGGGTGGACCACTGATATCTAAGTTTATAGAGGAACACAACCCACCAGCTCAAGGAGCAAGGCCTTCTAGTGAAATGATTGAAAGCAGTAATCATGTGTTTGACATTGATGGCAGTGTCTACCTACACAAGTGGAAGACCTCTCCATCTTGGGCTTCAAGTGCTTCTGTGAGCTTTTGGAAGAATGCATCAATAAGGCAGGGGGTGGTATTAAGTAAAAATATTGTTGTTGCTGATTCAGCTCTAGTGGAAAGAGCCACAGGAATATGCAAACAAAAATCGCAGGCAGCTGAAAAAACTCAAGCCACTATTGATGCTGCAATGCTTAAAGGGATACCCAGTAACATCGATCTATTCAAGGAACTTCTGCTTCCTCTGACCATAATTGCCACAAAATTTGAAAAACTTAGGCGCTGGGAGGAGCCATACTTGACTGTTTCTTTTCTTGCATTTTCCTATACAATCATTTTCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

601

Amino Acids

67.82

Weight (kDa)

8.52

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 524 - 601 6.7e-24 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 451
AccI GTMKAC 1 cut(s) 1449
AciI CCGC 2 cut(s) 65, 974
AclWI GGATC 1 cut(s) 1241
AcsI RAATTY 4 cut(s) 82, 246, 1022, 1724
AcuI CTGAAG 3 cut(s) 116, 455, 1205
AcvI CACGTG 1 cut(s) 1011
AfaI GTAC 1 cut(s) 567
AfeI AGCGCT 1 cut(s) 336
AfiI CCNNNNNNNGG 2 cut(s) 748, 1382
AflIII ACRYGT 1 cut(s) 1008
AgsI TTSAA 7 cut(s) 82, 331, 380, 1412, 1488, 1687, 1730
AhlI ACTAGT 2 cut(s) 133, 143
AjnI CCWGG 3 cut(s) 815, 820, 913
AleI CACNNNNGTG 1 cut(s) 1013
AloI GAACNNNNNNTCC 2 cut(s) 432, 464
Alw21I GWGCWC 1 cut(s) 1159
Alw26I GTCTC 3 cut(s) 27, 176, 982
AlwI GGATC 1 cut(s) 1241
Ama87I CYCGRG 1 cut(s) 995
Aor51HI AGCGCT 1 cut(s) 336
AoxI GGCC 3 cut(s) 813, 818, 1391
ApeKI GCWGC 5 cut(s) 260, 387, 863, 1616, 1646
ApoI RAATTY 4 cut(s) 82, 246, 1022, 1724
AseI ATTAAT 2 cut(s) 225, 1194
Asp700I GAANNNNTTC 1 cut(s) 326
AspLEI GCGC 2 cut(s) 337, 1743
AspS9I GGNCC 5 cut(s) 603, 944, 992, 1115, 1337
AsuHPI GGTGA 4 cut(s) 157, 650, 1174, 1307
AvaI CYCGRG 1 cut(s) 995
AvaII GGWCC 5 cut(s) 603, 944, 992, 1115, 1337
BaeI ACNNNNGTAYC 2 cut(s) 716, 749
BanII GRGCYC 1 cut(s) 1159
BbrPI CACGTG 1 cut(s) 1011
BbsI GAAGAC 2 cut(s) 104, 1472
Bbv12I GWGCWC 1 cut(s) 1159
BbvI GCAGC 5 cut(s) 247, 374, 875, 1628, 1633
BccI CCATC 2 cut(s) 1433, 1483
BciT130I CCWGG 3 cut(s) 817, 822, 915
BciVI GTATCC 1 cut(s) 1656
BcoDI GTCTC 3 cut(s) 27, 176, 982
BcuI ACTAGT 2 cut(s) 133, 143
BfaI CTAG 9 cut(s) 134, 144, 632, 1206, 1241, 1259, 1316, 1398, 1574
BfmI CTRYAG 2 cut(s) 273, 1211
BfoI RGCGCY 2 cut(s) 338, 1744
BfuAI ACCTGC 1 cut(s) 451
BfuI GTATCC 1 cut(s) 1656
BisI GCNGC 5 cut(s) 261, 388, 864, 1617, 1647
BlsI GCNGC 5 cut(s) 262, 389, 865, 1618, 1648
Bme1390I CCNGG 3 cut(s) 817, 822, 915
Bme18I GGWCC 5 cut(s) 603, 944, 992, 1115, 1337
BmeT110I CYCGRG 1 cut(s) 995
BmgT120I GGNCC 5 cut(s) 603, 944, 992, 1115, 1337
BmiI GGNNCC 5 cut(s) 441, 576, 772, 994, 1752
BmrFI CCNGG 3 cut(s) 817, 822, 915
BmrI ACTGGG 1 cut(s) 1662
BmsI GCATC 8 cut(s) 52, 163, 352, 400, 547, 660, 1526, 1633
BmuI ACTGGG 1 cut(s) 1662
BpiI GAAGAC 2 cut(s) 104, 1472
BpuEI CTTGAG 4 cut(s) 200, 677, 1365, 1614
Bsa29I ATCGAT 1 cut(s) 1677
BsaAI YACGTR 1 cut(s) 1011
BsaBI GATNNNNATC 1 cut(s) 423
BsaI GGTCTC 1 cut(s) 982
Bsc4I CCNNNNNNNGG 2 cut(s) 748, 1382
Bse1I ACTGG 4 cut(s) 157, 510, 1185, 1668
Bse3DI GCAATG 1 cut(s) 1656
Bse8I GATNNNNATC 1 cut(s) 423
BseBI CCWGG 3 cut(s) 817, 822, 915
BseCI ATCGAT 1 cut(s) 1677
BseGI GGATG 3 cut(s) 297, 415, 424
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 2 cut(s) 748, 1382
BseMI GCAATG 1 cut(s) 1656
BseMII CTCAG 3 cut(s) 738, 1277, 1322
BseNI ACTGG 4 cut(s) 157, 510, 1185, 1668
BseRI GAGGAG 2 cut(s) 764, 1763
BseXI GCAGC 5 cut(s) 247, 374, 875, 1628, 1633
BseYI CCCAGC 1 cut(s) 1743
BshFI GGCC 3 cut(s) 815, 820, 1393
BshVI ATCGAT 1 cut(s) 1677
BsiHKAI GWGCWC 1 cut(s) 1159
BsiHKCI CYCGRG 1 cut(s) 995
BslFI GGGAC 2 cut(s) 718, 978
BslI CCNNNNNNNGG 2 cut(s) 748, 1382
BsmAI GTCTC 3 cut(s) 27, 176, 982
BsmFI GGGAC 2 cut(s) 718, 978
BsmI GAATGC 2 cut(s) 446, 1519
BsnI GGCC 3 cut(s) 815, 820, 1393
Bso31I GGTCTC 1 cut(s) 982
BsoBI CYCGRG 1 cut(s) 995
Bsp1286I GDGCHC 1 cut(s) 1159
Bsp143I GATC 2 cut(s) 1246, 1678
BspACI CCGC 2 cut(s) 65, 974
BspANI GGCC 3 cut(s) 815, 820, 1393
BspCNI CTCAG 3 cut(s) 739, 1276, 1321
BspDI ATCGAT 1 cut(s) 1677
BspHI TCATGA 1 cut(s) 54
BspLI GGNNCC 5 cut(s) 441, 576, 772, 994, 1752
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 1 cut(s) 1241
BspQI GCTCTTC 1 cut(s) 1224
BspTNI GGTCTC 1 cut(s) 982
BsrDI GCAATG 1 cut(s) 1656
BsrI ACTGG 4 cut(s) 157, 510, 1185, 1668
BssMI GATC 2 cut(s) 1246, 1678
Bst2UI CCWGG 3 cut(s) 817, 822, 915
Bst4CI ACNGT 3 cut(s) 21, 610, 1765
Bst6I CTCTTC 1 cut(s) 1224
BstAPI GCANNNNNTGC 1 cut(s) 316
BstBAI YACGTR 1 cut(s) 1011
BstC8I GCNNGC 4 cut(s) 477, 868, 1065, 1614
BstDEI CTNAG 5 cut(s) 747, 1263, 1308, 1350, 1736
BstENI CCTNNNNNAGG 1 cut(s) 746
BstF5I GGATG 3 cut(s) 297, 415, 424
BstH2I RGCGCY 2 cut(s) 338, 1744
BstHHI GCGC 2 cut(s) 337, 1743
BstKTI GATC 2 cut(s) 1249, 1681
BstMAI GTCTC 3 cut(s) 27, 176, 982
BstMBI GATC 2 cut(s) 1246, 1678
BstMWI GCNNNNNNNGC 4 cut(s) 316, 1385, 1490, 1568
BstNI CCWGG 3 cut(s) 817, 822, 915
BstNSI RCATGY 1 cut(s) 1067
BstSCI CCNGG 3 cut(s) 815, 820, 913
BstSFI CTRYAG 2 cut(s) 273, 1211
BstV1I GCAGC 5 cut(s) 247, 374, 875, 1628, 1633
BstV2I GAAGAC 2 cut(s) 104, 1472
Bsu15I ATCGAT 1 cut(s) 1677
BsuI GTATCC 1 cut(s) 1656
BsuRI GGCC 3 cut(s) 815, 820, 1393
BsuTUI ATCGAT 1 cut(s) 1677
BtsCI GGATG 3 cut(s) 297, 415, 424
BtsI GCAGTG 1 cut(s) 1450
BtsIMutI CAGTG 5 cut(s) 517, 615, 1192, 1340, 1450
BveI ACCTGC 1 cut(s) 451
Cac8I GCNNGC 4 cut(s) 477, 868, 1065, 1614
CciI TCATGA 1 cut(s) 54
CfoI GCGC 2 cut(s) 337, 1743
Cfr13I GGNCC 5 cut(s) 603, 944, 992, 1115, 1337
ClaI ATCGAT 1 cut(s) 1677
Csp6I GTAC 1 cut(s) 566
CspCI CAANNNNNGTGG 2 cut(s) 1708, 1743
CviAII CATG 6 cut(s) 55, 87, 480, 1064, 1145, 1424
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 5 cut(s) 747, 1263, 1308, 1350, 1736
DpnI GATC 2 cut(s) 1248, 1680
DpnII GATC 2 cut(s) 1246, 1678
Eam1104I CTCTTC 1 cut(s) 1224
EarI CTCTTC 1 cut(s) 1224
Ecl136II GAGCTC 1 cut(s) 1157
Eco147I AGGCCT 1 cut(s) 1393
Eco24I GRGCYC 1 cut(s) 1159
Eco31I GGTCTC 1 cut(s) 982
Eco32I GATATC 1 cut(s) 1347
Eco47I GGWCC 5 cut(s) 603, 944, 992, 1115, 1337
Eco47III AGCGCT 1 cut(s) 336
Eco53kI GAGCTC 1 cut(s) 1157
Eco57I CTGAAG 3 cut(s) 116, 455, 1205
Eco72I CACGTG 1 cut(s) 1011
Eco88I CYCGRG 1 cut(s) 995
EcoICRI GAGCTC 1 cut(s) 1157
EcoNI CCTNNNNNAGG 1 cut(s) 746
EcoRI GAATTC 2 cut(s) 82, 246
EcoRII CCWGG 3 cut(s) 815, 820, 913
EcoRV GATATC 1 cut(s) 1347
EcoT22I ATGCAT 2 cut(s) 767, 1519
EcoT38I GRGCYC 1 cut(s) 1159
FaeI CATG 6 cut(s) 58, 90, 483, 1067, 1148, 1427
FalI AAGNNNNNCTT 4 cut(s) 397, 429, 489, 521
FaqI GGGAC 2 cut(s) 718, 978
FatI CATG 6 cut(s) 54, 86, 479, 1063, 1144, 1423
FauNDI CATATG 2 cut(s) 988, 1291
FblI GTMKAC 1 cut(s) 1449
Fnu4HI GCNGC 5 cut(s) 261, 388, 864, 1617, 1647
FokI GGATG 3 cut(s) 304, 411, 422
FriOI GRGCYC 1 cut(s) 1159
Fsp4HI GCNGC 5 cut(s) 261, 388, 864, 1617, 1647
FspBI CTAG 9 cut(s) 134, 144, 632, 1206, 1241, 1259, 1316, 1398, 1574
GlaI GCGC 2 cut(s) 336, 1742
GluI GCNGC 5 cut(s) 261, 388, 864, 1617, 1647
GsaI CCCAGC 1 cut(s) 1747
HaeII RGCGCY 2 cut(s) 338, 1744
HaeIII GGCC 3 cut(s) 815, 820, 1393
HhaI GCGC 2 cut(s) 337, 1743
Hin1II CATG 6 cut(s) 58, 90, 483, 1067, 1148, 1427
Hin6I GCGC 2 cut(s) 335, 1741
HinP1I GCGC 2 cut(s) 335, 1741
HincII GTYRAC 2 cut(s) 672, 1030
HindII GTYRAC 2 cut(s) 672, 1030
HindIII AAGCTT 2 cut(s) 106, 525
HinfI GANTC 9 cut(s) 347, 487, 731, 756, 887, 968, 998, 1133, 1565
HphI GGTGA 4 cut(s) 157, 650, 1174, 1307
Hpy166II GTNNAC 8 cut(s) 606, 672, 700, 1030, 1269, 1324, 1337, 1450
Hpy188I TCNGA 8 cut(s) 150, 418, 520, 537, 723, 748, 1251, 1707
Hpy188III TCNNGA 6 cut(s) 55, 179, 344, 497, 701, 995
Hpy8I GTNNAC 8 cut(s) 606, 672, 700, 1030, 1269, 1324, 1337, 1450
HpyAV CCTTC 4 cut(s) 430, 438, 698, 1404
HpyCH4III ACNGT 3 cut(s) 21, 610, 1765
HpyCH4IV ACGT 3 cut(s) 568, 784, 1010
HpyF10VI GCNNNNNNNGC 4 cut(s) 316, 1385, 1490, 1568
HpyF3I CTNAG 5 cut(s) 747, 1263, 1308, 1350, 1736
HpySE526I ACGT 3 cut(s) 568, 784, 1010
Hsp92II CATG 6 cut(s) 58, 90, 483, 1067, 1148, 1427
HspAI GCGC 2 cut(s) 335, 1741
Kzo9I GATC 2 cut(s) 1246, 1678
LguI GCTCTTC 1 cut(s) 1224
LmnI GCTCC 4 cut(s) 580, 1154, 1385, 1750
Lsp1109I GCAGC 5 cut(s) 247, 374, 875, 1628, 1633
LweI GCATC 8 cut(s) 52, 163, 352, 400, 547, 660, 1526, 1633
MaeI CTAG 9 cut(s) 134, 144, 632, 1206, 1241, 1259, 1316, 1398, 1574
MaeII ACGT 3 cut(s) 568, 784, 1010
MaeIII GTNAC 5 cut(s) 121, 163, 829, 1089, 1670
MalI GATC 2 cut(s) 1248, 1680
MboI GATC 2 cut(s) 1246, 1678
MboII GAAGA 7 cut(s) 109, 566, 720, 1136, 1211, 1477, 1522
MhlI GDGCHC 1 cut(s) 1159
MluCI AATT 7 cut(s) 82, 246, 370, 582, 1022, 1713, 1724
MlyI GAGTC 2 cut(s) 740, 1007
MmeI TCCRAC 3 cut(s) 879, 922, 1274
MnlI CCTC 8 cut(s) 742, 853, 1041, 1224, 1354, 1480, 1713, 1741
Mph1103I ATGCAT 2 cut(s) 767, 1519
MroXI GAANNNNTTC 1 cut(s) 326
MseI TTAA 7 cut(s) 113, 225, 624, 794, 1194, 1541, 1656
MslI CAYNNNNRTG 4 cut(s) 206, 470, 1013, 1149
MspA1I CMGCKG 2 cut(s) 866, 1619
MspR9I CCNGG 3 cut(s) 817, 822, 915
Mva1269I GAATGC 2 cut(s) 446, 1519
MvaI CCWGG 3 cut(s) 817, 822, 915
MwoI GCNNNNNNNGC 4 cut(s) 316, 1385, 1490, 1568
NdeI CATATG 2 cut(s) 988, 1291
NdeII GATC 2 cut(s) 1246, 1678
NlaIII CATG 6 cut(s) 58, 90, 483, 1067, 1148, 1427
NlaIV GGNNCC 5 cut(s) 441, 576, 772, 994, 1752
NmuCI GTSAC 1 cut(s) 163
NsiI ATGCAT 2 cut(s) 767, 1519
NspI RCATGY 1 cut(s) 1067
OliI CACNNNNGTG 1 cut(s) 1013
PaeI GCATGC 1 cut(s) 1067
PagI TCATGA 1 cut(s) 54
PceI AGGCCT 1 cut(s) 1393
PciSI GCTCTTC 1 cut(s) 1224
PctI GAATGC 2 cut(s) 446, 1519
PdmI GAANNNNTTC 1 cut(s) 326
PfeI GAWTC 7 cut(s) 347, 487, 756, 887, 968, 1133, 1565
PkrI GCNGC 5 cut(s) 262, 389, 865, 1618, 1648
PleI GAGTC 2 cut(s) 739, 1006
PmaCI CACGTG 1 cut(s) 1011
PmlI CACGTG 1 cut(s) 1011
PpsI GAGTC 2 cut(s) 739, 1006
Ppu21I YACGTR 1 cut(s) 1011
PshBI ATTAAT 2 cut(s) 225, 1194
Psp124BI GAGCTC 1 cut(s) 1159
Psp6I CCWGG 3 cut(s) 815, 820, 913
PspCI CACGTG 1 cut(s) 1011
PspFI CCCAGC 1 cut(s) 1743
PspGI CCWGG 3 cut(s) 815, 820, 913
PspN4I GGNNCC 5 cut(s) 441, 576, 772, 994, 1752
PspPI GGNCC 5 cut(s) 603, 944, 992, 1115, 1337
PvuII CAGCTG 2 cut(s) 866, 1619
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
RseI CAYNNNNRTG 4 cut(s) 206, 470, 1013, 1149
SacI GAGCTC 1 cut(s) 1159
SapI GCTCTTC 1 cut(s) 1224
SaqAI TTAA 7 cut(s) 113, 225, 624, 794, 1194, 1541, 1656
SatI GCNGC 5 cut(s) 261, 388, 864, 1617, 1647
Sau3AI GATC 2 cut(s) 1246, 1678
Sau96I GGNCC 5 cut(s) 603, 944, 992, 1115, 1337
SchI GAGTC 2 cut(s) 740, 1007
ScrFI CCNGG 3 cut(s) 817, 822, 915
SduI GDGCHC 1 cut(s) 1159
SfaNI GCATC 8 cut(s) 52, 163, 352, 400, 547, 660, 1526, 1633
SfcI CTRYAG 2 cut(s) 273, 1211
SinI GGWCC 5 cut(s) 603, 944, 992, 1115, 1337
SmiMI CAYNNNNRTG 4 cut(s) 206, 470, 1013, 1149
SmlI CTYRAG 4 cut(s) 179, 692, 1380, 1629
SmoI CTYRAG 4 cut(s) 179, 692, 1380, 1629
SpeI ACTAGT 2 cut(s) 133, 143
SphI GCATGC 1 cut(s) 1067
Sse9I AATT 7 cut(s) 82, 246, 370, 582, 1022, 1713, 1724
SseBI AGGCCT 1 cut(s) 1393
SsiI CCGC 2 cut(s) 65, 974
SspI AATATT 3 cut(s) 327, 383, 1552
SspMI CTAG 9 cut(s) 134, 144, 632, 1206, 1241, 1259, 1316, 1398, 1574
SstI GAGCTC 1 cut(s) 1159
StuI AGGCCT 1 cut(s) 1393
StyD4I CCNGG 3 cut(s) 815, 820, 913
TaaI ACNGT 3 cut(s) 21, 610, 1765
TaiI ACGT 3 cut(s) 571, 787, 1013
TaqI TCGA 1 cut(s) 1677
TasI AATT 7 cut(s) 82, 246, 370, 582, 1022, 1713, 1724
TfiI GAWTC 7 cut(s) 347, 487, 756, 887, 968, 1133, 1565
Tru1I TTAA 7 cut(s) 113, 225, 624, 794, 1194, 1541, 1656
Tru9I TTAA 7 cut(s) 113, 225, 624, 794, 1194, 1541, 1656
TscAI CASTG 5 cut(s) 517, 615, 1192, 1347, 1450
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 5 cut(s) 260, 387, 863, 1616, 1646
Tsp45I GTSAC 1 cut(s) 163
TspRI CASTG 5 cut(s) 517, 615, 1192, 1347, 1450
VpaK11BI GGWCC 5 cut(s) 603, 944, 992, 1115, 1337
VspI ATTAAT 2 cut(s) 225, 1194
XagI CCTNNNNNAGG 1 cut(s) 746
XapI RAATTY 4 cut(s) 82, 246, 1022, 1724
XceI RCATGY 1 cut(s) 1067
XmiI GTMKAC 1 cut(s) 1449
XmnI GAANNNNTTC 1 cut(s) 326
XspI CTAG 9 cut(s) 134, 144, 632, 1206, 1241, 1259, 1316, 1398, 1574
Zsp2I ATGCAT 2 cut(s) 767, 1519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.