Rorug07G0212000

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003186
Physical Location & Seq
Reverse (-)
18714072 .. 18717056
2985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0212000.1

Sequence Viewer

Length: 1437 bp
ATGAGGGTTGTAATGGGGTTGCAGCAGTTGGAATCATCGAGAACAATTGAACAGCTAAGCGGTAGCCATTCTGAGGAAAGATTGGGAAGATTGTCCTTGGAAAATGGGCCCGAGTATAAGAGACCCAGAGTCTCTGCTATTCGTGACTTCCCCCCTGGGTGTGGACGGTATGCAACTTCAGTTGGTACTTCTAATGGCATTGGTATGGACATGACTGTGGCTAAAAAATATCCTCCTCAAAGAATAGTAGAATATGTTCGGGATTTCCCACCTCAATGTGGAATAAATATATGTTTCGAGGCCAAGGCTTTTGATGATGTGAAAAAATCAGAAGTGGATGTTCAAGATGAAGAGCTTCACAACAGTGAATTGAAAGCAGATGTTTCTCAAGTCAATGGTGGCAACTTTCTACCTAGTAATATGATGGAACGGAGGATGGAAGGTCCAAAGGAGAAGTCTTTTGATATATCTAATTCTCATAACCATCTGCTTGAAGAAGATGTTGAGAGTTCAGAACTGAAATTGAATAAAGTAATTCCGAACTTGAAGCCTGCATCACATCCTTCCTGGTGGAAGGGAAAAGTGGCCCACAAGTCTAAACCAGGAGGAGGTATAAGTGAAAGACAGGGAGAGAAACCTGATTCAAAGCTTCAGCTAGAAAGATCTAAAGATGCTTGGAGGACAAAAGTTGGCCCAGAGATTGGAGGACACTCTAAAAAGAATATTCATTCAATAGCAGGGAAGACTACTGGTTTTGGTCAGGCTAGTTCTAGCAGCAGCCTTCATGATAATAACACGGATGCCATCCGAAAAAAGGTGACAGAGACGTTGGGTCGTTTCAAAGCTCTTTGTAGACAACTGGAATATGACAAGTCAAAGGAAGGAGGAACTCCACTCAGAAGGGTCGATTTAAAAGCTGCAAAGATTCTCAAGAAGGAAGGAAAACATATAAATACTGGCAAACAAATCTTGGGACATTTCCCCGGTGTTGAAGTTGGCGATGAATTTCATTACAGGATTGAACTTAATATTGTTGGCTTACATTACCCAACTCAAGGTGGTATAGATTATGCAACTTTTGGTGGGAAGATGCTTGCAACTAGTATTGTTGCATCTGGGGGATATGCTGATGATTTGCATAATTTGAATTCCTTGACTTACACGGGCCAAGGAGGAAATGTGATGCATCCGGATAAGGAACCTGAAGATCAAAAGCTTCAACGGGGAAACCTTGCTTTGAAGAATAGCATGCATGCAAAGACTCCTGTTAGAATGATCCGTGGTTCTGAATCATCAGATGGAAGAACTAAATATTATATATATGATGGACTATATTTGGTAGTGAAGTGTTGGAAGGAAATGGGGCCTCATGGTAAGCTTGTTTTCAAGTTTCAACTAGACAGAATTGAGGGTCAGAGCTTGCTACGAAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

478

Amino Acids

53.21

Weight (kDa)

9.24

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 324 - 472 1.1e-51 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 701
AccI GTMKAC 1 cut(s) 853
AccIII TCCGGA 1 cut(s) 1189
AciI CCGC 1 cut(s) 60
AclWI GGATC 1 cut(s) 1270
AcsI RAATTY 2 cut(s) 1004, 1147
AcuI CTGAAG 3 cut(s) 162, 635, 1224
AfaI GTAC 1 cut(s) 187
AfiI CCNNNNNNNGG 7 cut(s) 73, 161, 278, 608, 701, 814, 1055
AhdI GACNNNNNGTC 2 cut(s) 128, 831
AhlI ACTAGT 1 cut(s) 1100
AjnI CCWGG 3 cut(s) 154, 566, 601
AleI CACNNNNGTG 1 cut(s) 363
AluBI AGCT 9 cut(s) 55, 355, 649, 655, 845, 917, 1216, 1378, 1419
AluI AGCT 9 cut(s) 55, 355, 649, 655, 845, 917, 1216, 1378, 1419
Alw26I GTCTC 3 cut(s) 115, 136, 818
AlwI GGATC 1 cut(s) 1270
Ama87I CYCGRG 1 cut(s) 110
Aor13HI TCCGGA 1 cut(s) 1189
AoxI GGCC 6 cut(s) 107, 300, 585, 691, 1165, 1364
ApaI GGGCCC 1 cut(s) 111
ApeKI GCWGC 4 cut(s) 22, 774, 777, 917
ApoI RAATTY 2 cut(s) 1004, 1147
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
Asp700I GAANNNNTTC 2 cut(s) 255, 354
AspS9I GGNCC 7 cut(s) 107, 108, 443, 586, 692, 1165, 1364
AsuC2I CCSGG 1 cut(s) 984
AsuHPI GGTGA 1 cut(s) 829
AvaI CYCGRG 1 cut(s) 110
AvaII GGWCC 1 cut(s) 443
BaeGI GKGCMC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 749
BbvI GCAGC 4 cut(s) 34, 786, 789, 904
BccI CCATC 6 cut(s) 418, 430, 492, 812, 1292, 1319
BciT130I CCWGG 3 cut(s) 156, 568, 603
BcnI CCSGG 1 cut(s) 984
BcoDI GTCTC 3 cut(s) 115, 136, 818
BcuI ACTAGT 1 cut(s) 1100
BfaI CTAG 6 cut(s) 414, 656, 765, 771, 1101, 1397
BglII AGATCT 1 cut(s) 662
BisI GCNGC 4 cut(s) 23, 775, 778, 918
BlpI GCTNAGC 1 cut(s) 56
BlsI GCNGC 4 cut(s) 24, 776, 779, 919
Bme1390I CCNGG 4 cut(s) 156, 568, 603, 984
Bme18I GGWCC 1 cut(s) 443
BmeRI GACNNNNNGTC 2 cut(s) 128, 831
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 7 cut(s) 107, 108, 443, 586, 692, 1165, 1364
BmiI GGNNCC 3 cut(s) 109, 1200, 1365
BmrFI CCNGG 4 cut(s) 156, 568, 603, 984
BmsI GCATC 7 cut(s) 563, 661, 790, 1080, 1121, 1173, 1195
BpiI GAAGAC 1 cut(s) 749
Bpu1102I GCTNAGC 1 cut(s) 56
BpuEI CTTGAG 3 cut(s) 372, 914, 1038
BpuMI CCSGG 1 cut(s) 984
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 7 cut(s) 96, 154, 155, 303, 982, 1168, 1279
BsaWI WCCGGW 1 cut(s) 1189
BsaXI ACNNNNNCTCC 2 cut(s) 1164, 1194
Bsc4I CCNNNNNNNGG 7 cut(s) 73, 161, 278, 608, 701, 814, 1055
Bse1I ACTGG 3 cut(s) 754, 864, 961
BseAI TCCGGA 1 cut(s) 1189
BseBI CCWGG 3 cut(s) 156, 568, 603
BseDI CCNNGG 7 cut(s) 96, 154, 155, 303, 982, 1168, 1279
BseGI GGATG 6 cut(s) 343, 441, 559, 804, 805, 1186
BseLI CCNNNNNNNGG 7 cut(s) 73, 161, 278, 608, 701, 814, 1055
BseMII CTCAG 2 cut(s) 63, 910
BseNI ACTGG 3 cut(s) 754, 864, 961
BseRI GAGGAG 2 cut(s) 225, 621
BseSI GKGCMC 1 cut(s) 111
BseXI GCAGC 4 cut(s) 34, 786, 789, 904
BshFI GGCC 6 cut(s) 109, 302, 587, 693, 1167, 1366
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 2 cut(s) 984, 1190
BslFI GGGAC 1 cut(s) 987
BslI CCNNNNNNNGG 7 cut(s) 73, 161, 278, 608, 701, 814, 1055
BsmAI GTCTC 3 cut(s) 115, 136, 818
BsmBI CGTCTC 1 cut(s) 818
BsmFI GGGAC 1 cut(s) 987
BsnI GGCC 6 cut(s) 109, 302, 587, 693, 1167, 1366
Bso31I GGTCTC 1 cut(s) 115
BsoBI CYCGRG 1 cut(s) 110
Bsp120I GGGCCC 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 111
Bsp13I TCCGGA 1 cut(s) 1189
Bsp143I GATC 3 cut(s) 662, 1207, 1275
Bsp1720I GCTNAGC 1 cut(s) 56
BspACI CCGC 1 cut(s) 60
BspANI GGCC 6 cut(s) 109, 302, 587, 693, 1167, 1366
BspCNI CTCAG 2 cut(s) 64, 909
BspEI TCCGGA 1 cut(s) 1189
BspHI TCATGA 1 cut(s) 784
BspLI GGNNCC 3 cut(s) 109, 1200, 1365
BspPI GGATC 1 cut(s) 1270
BspQI GCTCTTC 1 cut(s) 345
BspTNI GGTCTC 1 cut(s) 115
BsrI ACTGG 3 cut(s) 754, 864, 961
BssECI CCNNGG 7 cut(s) 96, 154, 155, 303, 982, 1168, 1279
BssMI GATC 3 cut(s) 662, 1207, 1275
BssT1I CCWWGG 3 cut(s) 96, 303, 1168
Bst2UI CCWGG 3 cut(s) 156, 568, 603
Bst4CI ACNGT 3 cut(s) 168, 217, 365
Bst6I CTCTTC 1 cut(s) 345
BstC8I GCNNGC 5 cut(s) 552, 1095, 1250, 1254, 1421
BstDEI CTNAG 3 cut(s) 56, 72, 896
BstDSI CCRYGG 1 cut(s) 1279
BstF5I GGATG 6 cut(s) 343, 441, 559, 804, 805, 1186
BstKTI GATC 3 cut(s) 665, 1210, 1278
BstMAI GTCTC 3 cut(s) 115, 136, 818
BstMBI GATC 3 cut(s) 662, 1207, 1275
BstNI CCWGG 3 cut(s) 156, 568, 603
BstNSI RCATGY 2 cut(s) 1252, 1256
BstSCI CCNGG 4 cut(s) 154, 566, 601, 982
BstSLI GKGCMC 1 cut(s) 111
BstV1I GCAGC 4 cut(s) 34, 786, 789, 904
BstV2I GAAGAC 1 cut(s) 749
BstX2I RGATCY 1 cut(s) 662
BstYI RGATCY 1 cut(s) 662
BsuRI GGCC 6 cut(s) 109, 302, 587, 693, 1167, 1366
BtgI CCRYGG 1 cut(s) 1279
BtgZI GCGATG 1 cut(s) 1014
BtsCI GGATG 6 cut(s) 343, 441, 559, 804, 805, 1186
BtsIMutI CAGTG 1 cut(s) 370
Cac8I GCNNGC 5 cut(s) 552, 1095, 1250, 1254, 1421
CciI TCATGA 1 cut(s) 784
Cfr13I GGNCC 7 cut(s) 107, 108, 443, 586, 692, 1165, 1364
Csp6I GTAC 1 cut(s) 186
CviAII CATG 5 cut(s) 211, 785, 1249, 1253, 1370
CviQI GTAC 1 cut(s) 186
DdeI CTNAG 3 cut(s) 56, 72, 896
DpnI GATC 3 cut(s) 664, 1209, 1277
DpnII GATC 3 cut(s) 662, 1207, 1275
DraI TTTAAA 1 cut(s) 912
DriI GACNNNNNGTC 2 cut(s) 128, 831
Eam1104I CTCTTC 1 cut(s) 345
Eam1105I GACNNNNNGTC 2 cut(s) 128, 831
EarI CTCTTC 1 cut(s) 345
Eco130I CCWWGG 3 cut(s) 96, 303, 1168
Eco24I GRGCYC 1 cut(s) 111
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 443
Eco57I CTGAAG 3 cut(s) 162, 635, 1224
Eco88I CYCGRG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 1364
EcoRI GAATTC 1 cut(s) 1147
EcoRII CCWGG 3 cut(s) 154, 566, 601
EcoT14I CCWWGG 3 cut(s) 96, 303, 1168
EcoT22I ATGCAT 2 cut(s) 1188, 1254
EcoT38I GRGCYC 1 cut(s) 111
ErhI CCWWGG 3 cut(s) 96, 303, 1168
Esp3I CGTCTC 1 cut(s) 818
FaeI CATG 5 cut(s) 214, 788, 1252, 1256, 1373
FaqI GGGAC 1 cut(s) 987
FatI CATG 5 cut(s) 210, 784, 1248, 1252, 1369
FblI GTMKAC 1 cut(s) 853
Fnu4HI GCNGC 4 cut(s) 23, 775, 778, 918
FokI GGATG 6 cut(s) 350, 448, 546, 791, 812, 1173
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 4 cut(s) 23, 775, 778, 918
FspBI CTAG 6 cut(s) 414, 656, 765, 771, 1101, 1397
GluI GCNGC 4 cut(s) 23, 775, 778, 918
HaeIII GGCC 6 cut(s) 109, 302, 587, 693, 1167, 1366
HapII CCGG 2 cut(s) 984, 1190
Hin1II CATG 5 cut(s) 214, 788, 1252, 1256, 1373
HindIII AAGCTT 3 cut(s) 647, 1214, 1376
HinfI GANTC 6 cut(s) 32, 129, 641, 925, 1261, 1289
HpaII CCGG 2 cut(s) 984, 1190
HphI GGTGA 1 cut(s) 829
Hpy166II GTNNAC 2 cut(s) 164, 854
Hpy188I TCNGA 9 cut(s) 73, 331, 514, 540, 809, 899, 1288, 1297, 1416
Hpy188III TCNNGA 7 cut(s) 39, 143, 260, 344, 785, 931, 1190
Hpy8I GTNNAC 2 cut(s) 164, 854
HpyAV CCTTC 9 cut(s) 434, 568, 573, 791, 875, 894, 928, 932, 1348
HpyCH4III ACNGT 3 cut(s) 168, 217, 365
HpyCH4IV ACGT 1 cut(s) 827
HpyF3I CTNAG 3 cut(s) 56, 72, 896
HpySE526I ACGT 1 cut(s) 827
Hsp92II CATG 5 cut(s) 214, 788, 1252, 1256, 1373
Kpn2I TCCGGA 1 cut(s) 1189
Kzo9I GATC 3 cut(s) 662, 1207, 1275
LguI GCTCTTC 1 cut(s) 345
Lsp1109I GCAGC 4 cut(s) 34, 786, 789, 904
LweI GCATC 7 cut(s) 563, 661, 790, 1080, 1121, 1173, 1195
MaeI CTAG 6 cut(s) 414, 656, 765, 771, 1101, 1397
MaeII ACGT 1 cut(s) 827
MaeIII GTNAC 2 cut(s) 143, 817
MalI GATC 3 cut(s) 664, 1209, 1277
MboI GATC 3 cut(s) 662, 1207, 1275
MboII GAAGA 9 cut(s) 99, 362, 506, 509, 754, 1099, 1217, 1252, 1314
MfeI CAATTG 1 cut(s) 45
MflI RGATCY 1 cut(s) 662
MhlI GDGCHC 1 cut(s) 111
MluCI AATT 9 cut(s) 45, 368, 472, 521, 534, 1004, 1141, 1147, 1404
MlyI GAGTC 2 cut(s) 138, 1255
MmeI TCCRAC 2 cut(s) 9, 1331
Mph1103I ATGCAT 2 cut(s) 1188, 1254
MroI TCCGGA 1 cut(s) 1189
MroXI GAANNNNTTC 2 cut(s) 255, 354
MseI TTAA 2 cut(s) 911, 1026
MslI CAYNNNNRTG 4 cut(s) 203, 215, 274, 363
MspI CCGG 2 cut(s) 984, 1190
MspR9I CCNGG 4 cut(s) 156, 568, 603, 984
MunI CAATTG 1 cut(s) 45
MvaI CCWGG 3 cut(s) 156, 568, 603
NciI CCSGG 1 cut(s) 984
NdeII GATC 3 cut(s) 662, 1207, 1275
NlaIII CATG 5 cut(s) 214, 788, 1252, 1256, 1373
NlaIV GGNNCC 3 cut(s) 109, 1200, 1365
NmuCI GTSAC 2 cut(s) 143, 817
NsiI ATGCAT 2 cut(s) 1188, 1254
NspI RCATGY 2 cut(s) 1252, 1256
OliI CACNNNNGTG 1 cut(s) 363
PaeI GCATGC 2 cut(s) 1252, 1256
PagI TCATGA 1 cut(s) 784
PasI CCCWGGG 1 cut(s) 155
PciSI GCTCTTC 1 cut(s) 345
PdmI GAANNNNTTC 2 cut(s) 255, 354
PfeI GAWTC 4 cut(s) 32, 641, 925, 1289
PflMI CCANNNNNTGG 1 cut(s) 701
PkrI GCNGC 4 cut(s) 24, 776, 779, 919
PleI GAGTC 2 cut(s) 137, 1255
PpsI GAGTC 2 cut(s) 137, 1255
Psp6I CCWGG 3 cut(s) 154, 566, 601
PspGI CCWGG 3 cut(s) 154, 566, 601
PspN4I GGNNCC 3 cut(s) 109, 1200, 1365
PspOMI GGGCCC 1 cut(s) 107
PspPI GGNCC 7 cut(s) 107, 108, 443, 586, 692, 1165, 1364
PsrI GAACNNNNNNTAC 2 cut(s) 240, 272
PsuI RGATCY 1 cut(s) 662
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
RseI CAYNNNNRTG 4 cut(s) 203, 215, 274, 363
SapI GCTCTTC 1 cut(s) 345
SaqAI TTAA 2 cut(s) 911, 1026
SatI GCNGC 4 cut(s) 23, 775, 778, 918
Sau3AI GATC 3 cut(s) 662, 1207, 1275
Sau96I GGNCC 7 cut(s) 107, 108, 443, 586, 692, 1165, 1364
SchI GAGTC 2 cut(s) 138, 1255
ScrFI CCNGG 4 cut(s) 156, 568, 603, 984
SduI GDGCHC 1 cut(s) 111
SfaNI GCATC 7 cut(s) 563, 661, 790, 1080, 1121, 1173, 1195
SinI GGWCC 1 cut(s) 443
SmiMI CAYNNNNRTG 4 cut(s) 203, 215, 274, 363
SmlI CTYRAG 3 cut(s) 387, 929, 1053
SmoI CTYRAG 3 cut(s) 387, 929, 1053
SpeI ACTAGT 1 cut(s) 1100
SphI GCATGC 2 cut(s) 1252, 1256
Sse9I AATT 9 cut(s) 45, 368, 472, 521, 534, 1004, 1141, 1147, 1404
SsiI CCGC 1 cut(s) 60
SspI AATATT 3 cut(s) 724, 1030, 1313
SspMI CTAG 6 cut(s) 414, 656, 765, 771, 1101, 1397
StyD4I CCNGG 4 cut(s) 154, 566, 601, 982
StyI CCWWGG 3 cut(s) 96, 303, 1168
TaaI ACNGT 3 cut(s) 168, 217, 365
TaiI ACGT 1 cut(s) 830
TaqI TCGA 3 cut(s) 38, 297, 906
TasI AATT 9 cut(s) 45, 368, 472, 521, 534, 1004, 1141, 1147, 1404
TfiI GAWTC 4 cut(s) 32, 641, 925, 1289
Tru1I TTAA 2 cut(s) 911, 1026
Tru9I TTAA 2 cut(s) 911, 1026
TscAI CASTG 1 cut(s) 370
TseFI GTSAC 2 cut(s) 143, 817
TseI GCWGC 4 cut(s) 22, 774, 777, 917
Tsp45I GTSAC 2 cut(s) 143, 817
TspDTI ATGAA 5 cut(s) 363, 716, 773, 998, 1017
TspGWI ACGGA 3 cut(s) 445, 812, 1268
TspRI CASTG 1 cut(s) 370
Van91I CCANNNNNTGG 1 cut(s) 701
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 2 cut(s) 1004, 1147
XceI RCATGY 2 cut(s) 1252, 1256
XmiI GTMKAC 1 cut(s) 853
XmnI GAANNNNTTC 2 cut(s) 255, 354
XspI CTAG 6 cut(s) 414, 656, 765, 771, 1101, 1397
Zsp2I ATGCAT 2 cut(s) 1188, 1254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.