MD15G1421600.v1.1

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003186
Physical Location & Seq
Forward (+)
52329900 .. 52338393
8494 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1421600.v1.1.491

Sequence Viewer

Length: 2484 bp
ATGCTGTCGAAAATCTCAGTTACCCAGTTCAAAACATCACCTGCTGCACCCAACCCTGGATTTTCATGGCGTGGGAATCGTCCTGTGCTTGGATATTGCGGCAGGAGTTCTTCAGAGCGTAACAAGTTGCGGTTCAAGATTGTGGCGCAGTCTCCGGGGGATGGGTGGAAGATCAACGACGTTGATGCAAATGCAGTGCAAGAAAAGTTGAATTCATGGCTAGTAAAGACGCAAAGCTTTATAAATGAAGTGGCATCTCCATTGACAAGAACTGGCCAGACTAAAAAGCCTGTCACTAGAGATGCTTTAGAGACACAAGATATGGAGGACATATTTATGGCAGAACAGACTATTAATAGCAGAACACCAAATGGAATTCTCTCTTTAGCTGCTGTTGTATCTATAGAGCAATTTAGCAGGATGAATGGATTGACGGGGAAGAAAATGCAGAATATATTTAAAGCACTTGTCTCTGAGTCTACATATAATGATGCCCGCAATTTGGTGGAATACTGCTGCTTCAGGTTTCTGTCAAGGGATAATTCTGATATTCATCCTTCCCTCAAGGAACCTCCATTCCAAAGACTCATATTTATAACTATGCTTGCATGGGAGAACCCTTATCGGAAAGATCTTGCTAATGGTTCGGAGAAAGCTTCTTTTCAGAGAAAGCTTGTTAGAGAAGAGGCTTTTGTTCGTATGGCTCCTGCTATTTCTGGTGTGGCTGATAGGTCAACTGTGCATAATCTATTTAAGGCTCTTGCCGGTGATGAACAGGGCATCTCTCTGAGCACGTGGTTGACTTATGTTGATGAACTTCTCAAGATTCACGAAGGAAGAAAATCATACCAGACTCGACCAAGTTCCAACCTTTCTGAGGAGAGAATTTTATGCATTGGTACCAGCAGGAAGCGGCCTGTCCTAAAATGGGAGAATAATATGGCATGGCCAGGAACAGTTACGTTAACTGATAAAGCAATTTATTTTGAGGCAGTTGGCCTTGTTGGGCAAAAGGATTCTATAAGATTGGATCTTACGAAACAAGAATTACAAGTTAAGAAAGCAAAAGTTGGACCTTTTGGTTCGGTTCTCTTCGACTCTGCAGTCTCTGTATCATATGGTCCCAAATCAGAAACATGGGTGCTTGAATTTGTTGACTTAGGTGGAGAAATGAGGCGAGACGTGTGGCATGCGTTTATTAGTGAAATTAATGCTGTACACAAGTTTATCCATAATTATGGACCGGAGGAAGATGATGAAGCCAAGTTTCATGTTTATGGCGCTCACAAAGGGAAGGAAAGAGCTATGACCAGTGCCATTAATAGTATAGCTAGACTACAGGCTCTTCAATTTATGAGGAAGTTACTTGATGATCCCACCAAACTTGTTCAGTTTACATATTTACAATATGTACCCTTTGGTGATGTAGTTTCTCAGACTCTAGCAGTTAATTATTGGGGTGGACCACTGATAACAAAGTATTTAGAGGCAGACAACCCACTAGCTCAAGGAACGAGTGCTTCCAATGAAATGATTGAAAGCAGCAGCAATCATGTGTTTGACATAGATGGCAGTGTTTACTTGCACAAATGGAAGAGATCTCCATGTTGGGCTTCAAGTTCTTCAGTTAGCTTTTGGAAGAATACTTCAACAAGACTGGGGCTAGTATTGAGTAAGAATCTTGTCGTTGCGGATGTGGCTCTTGTTGAAAGGGCAACAAGAACTTGCAAACAAAAATGGCAGGAAGCTGAAACAACTCAAGCCACAATTGATGCTGCAACCCTTAAGGGAATACCCAGTAACATTGATCTATTTAAGGAACTTCTGCTTCCTCTGGCCATGACTGCAACAAACTTTGAAAAACTTAGGCGCTGGGAGGAGCCCCACTTGACAATTTCTTTTCTTTCCCTTGCGTATACAGTCATTTTCAGGAATTTGCTGTCATATGTATTCCCAACAGCATTGATTATTTTGGCAACTGTCATGCTGACGTTGAAGGGGCTAAAGGAGCAAGGCCGCCTTGGACGATCATTTGGAAAAATTACTATCCGTGATCAGCCTCCTTCAAATACCATTGAAAAAATTATAGCTGTAAAAGACGCTATGCATGATGTGGAAAGTTATTTACAGAATCTGAACGTCATGCTTCTGAAAATACATACAATTTTCCTTTCGGGTCAGCCTCAGATAACGACTGAGGTTGCTCTGGTGTTGTTATCTTCTGCAACCATTCTCCTCATTTTCCCCCTCAAATTTGTTCTTGCCTTTTTTATCTTCGATCTGTTCACACGAGAGCTTGAGTTCAGGAGGGAAATGGTTAAAAGGTTTATGAAATTCTTGAAGGAGCGTTGGGACACAGTGCCTGCAGCCCCTGTAGTTGTATTACCTTTTGGAAGTGATGAGTCCGTACCAAAACCTAATGGAAAGGAAAGCAAGGATCCAGACGAGTCAGAAAGAAGCCTTGGCAGCAGCAGTTCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

828

Amino Acids

93.08

Weight (kDa)

9.06

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 566 - 797 1.9e-83 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 242, 596
AarI CACCTGC 1 cut(s) 49
AasI GACNNNNNNGTC 1 cut(s) 1103
Acc36I ACCTGC 1 cut(s) 49
Acc65I GGTACC 1 cut(s) 899
AccB1I GGYRCC 1 cut(s) 899
AccI GTMKAC 2 cut(s) 479, 1916
AciI CCGC 6 cut(s) 99, 130, 496, 913, 1691, 2017
AclWI GGATC 4 cut(s) 1038, 1367, 2432, 2445
AcoI YGGCCR 3 cut(s) 274, 947, 1836
AcsI RAATTY 7 cut(s) 211, 375, 885, 1148, 1933, 2252, 2333
AcuI CTGAAG 3 cut(s) 96, 505, 1608
AcvI CACGTG 1 cut(s) 795
AfaI GTAC 4 cut(s) 901, 1218, 1413, 2409
AfiI CCNNNNNNNGG 6 cut(s) 56, 89, 161, 502, 877, 928
AflII CTTAAG 1 cut(s) 1784
AflIII ACRYGT 1 cut(s) 1182
AjiI CACGTC 1 cut(s) 1183
AjnI CCWGG 2 cut(s) 55, 949
AjuI GAANNNNNNNTTGG 2 cut(s) 2445, 2477
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
Alw21I GWGCWC 1 cut(s) 794
Alw26I GTCTC 5 cut(s) 156, 305, 475, 1111, 1173
AlwI GGATC 4 cut(s) 1038, 1367, 2432, 2445
AlwNI CAGNNNCTG 4 cut(s) 1109, 2134, 2363, 2372
AoxI GGCC 6 cut(s) 274, 914, 947, 997, 1836, 2014
ApeKI GCWGC 9 cut(s) 44, 389, 516, 1542, 1545, 1775, 2366, 2466, 2469
ApoI RAATTY 7 cut(s) 211, 375, 885, 1148, 1933, 2252, 2333
AseI ATTAAT 3 cut(s) 354, 1209, 1320
Asp718I GGTACC 1 cut(s) 899
AspLEI GCGC 3 cut(s) 148, 1283, 1872
AspS9I GGNCC 4 cut(s) 1073, 1121, 1241, 1463
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 3 cut(s) 30, 779, 1433
AvaII GGWCC 4 cut(s) 1073, 1121, 1241, 1463
BalI TGGCCA 3 cut(s) 276, 949, 1838
BamHI GGATCC 1 cut(s) 2437
BanI GGYRCC 1 cut(s) 899
BanII GRGCYC 1 cut(s) 1884
BauI CACGAG 1 cut(s) 2289
BbrPI CACGTG 1 cut(s) 795
Bbv12I GWGCWC 1 cut(s) 794
BbvI GCAGC 8 cut(s) 31, 376, 503, 1554, 1557, 1762, 2378, 2478
BccI CCATC 2 cut(s) 155, 1562
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BciT130I CCWGG 2 cut(s) 57, 951
BclI TGATCA 1 cut(s) 2053
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 5 cut(s) 156, 305, 475, 1111, 1173
BfaI CTAG 6 cut(s) 221, 297, 1332, 1442, 1502, 1664
BfmI CTRYAG 5 cut(s) 402, 1101, 1337, 2364, 2373
BfoI RGCGCY 2 cut(s) 1284, 1873
BfrI CTTAAG 1 cut(s) 1784
BfuAI ACCTGC 1 cut(s) 49
BglII AGATCT 2 cut(s) 631, 1598
Bme1390I CCNGG 3 cut(s) 57, 156, 951
Bme18I GGWCC 4 cut(s) 1073, 1121, 1241, 1463
BmgBI CACGTC 1 cut(s) 1183
BmgT120I GGNCC 4 cut(s) 1073, 1121, 1241, 1463
BmiI GGNNCC 6 cut(s) 570, 705, 901, 1123, 1881, 2439
BmrFI CCNGG 3 cut(s) 57, 156, 951
BmrI ACTGGG 3 cut(s) 19, 1667, 1791
BmsI GCATC 6 cut(s) 175, 263, 292, 481, 789, 1762
BmuI ACTGGG 3 cut(s) 19, 1667, 1791
BpuEI CTTGAG 5 cut(s) 548, 806, 1491, 1743, 2318
BpuMI CCSGG 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 795
BsaBI GATNNNNATC 1 cut(s) 552
BsaJI CCNNGG 4 cut(s) 55, 155, 2020, 2461
BsaWI WCCGGW 1 cut(s) 1243
Bsc4I CCNNNNNNNGG 6 cut(s) 56, 89, 161, 502, 877, 928
Bse118I RCCGGY 1 cut(s) 764
Bse1I ACTGG 5 cut(s) 25, 277, 1311, 1662, 1797
Bse8I GATNNNNATC 1 cut(s) 552
BseBI CCWGG 2 cut(s) 57, 951
BseDI CCNNGG 4 cut(s) 55, 155, 2020, 2461
BseGI GGATG 4 cut(s) 166, 426, 553, 1699
BseJI GATNNNNATC 1 cut(s) 552
BseLI CCNNNNNNNGG 6 cut(s) 56, 89, 161, 502, 877, 928
BseMII CTCAG 7 cut(s) 30, 465, 779, 867, 1448, 2187, 2198
BseNI ACTGG 5 cut(s) 25, 277, 1311, 1662, 1797
BseRI GAGGAG 3 cut(s) 893, 1892, 2225
BseXI GCAGC 8 cut(s) 31, 376, 503, 1554, 1557, 1762, 2378, 2478
BseYI CCCAGC 1 cut(s) 1872
BsgI GTGCAG 1 cut(s) 30
BshFI GGCC 6 cut(s) 276, 916, 949, 999, 1838, 2016
BshNI GGYRCC 1 cut(s) 899
BsiHKAI GWGCWC 1 cut(s) 794
BsiSI CCGG 3 cut(s) 155, 765, 1244
BslFI GGGAC 2 cut(s) 1107, 2366
BslI CCNNNNNNNGG 6 cut(s) 56, 89, 161, 502, 877, 928
BsmAI GTCTC 5 cut(s) 156, 305, 475, 1111, 1173
BsmBI CGTCTC 1 cut(s) 1173
BsmFI GGGAC 2 cut(s) 1107, 2366
BsnI GGCC 6 cut(s) 276, 916, 949, 999, 1838, 2016
Bsp1286I GDGCHC 2 cut(s) 794, 1884
Bsp1407I TGTACA 1 cut(s) 1216
BspACI CCGC 6 cut(s) 99, 130, 496, 913, 1691, 2017
BspANI GGCC 6 cut(s) 276, 916, 949, 999, 1838, 2016
BspCNI CTCAG 7 cut(s) 29, 466, 780, 868, 1447, 2188, 2197
BspLI GGNNCC 6 cut(s) 570, 705, 901, 1123, 1881, 2439
BspMAI CTGCAG 2 cut(s) 1105, 2368
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 4 cut(s) 1038, 1367, 2432, 2445
BspQI GCTCTTC 1 cut(s) 1350
BspT107I GGYRCC 1 cut(s) 899
BspTI CTTAAG 1 cut(s) 1784
BsrFI RCCGGY 1 cut(s) 764
BsrGI TGTACA 1 cut(s) 1216
BsrI ACTGG 5 cut(s) 25, 277, 1311, 1662, 1797
BssAI RCCGGY 1 cut(s) 764
BssECI CCNNGG 4 cut(s) 55, 155, 2020, 2461
BssNAI GTATAC 1 cut(s) 1917
BssSI CACGAG 1 cut(s) 2289
BssT1I CCWWGG 2 cut(s) 2020, 2461
Bst1107I GTATAC 1 cut(s) 1917
Bst2BI CACGAG 1 cut(s) 2289
Bst2UI CCWGG 2 cut(s) 57, 951
Bst4CI ACNGT 5 cut(s) 739, 958, 1921, 1981, 2359
Bst6I CTCTTC 4 cut(s) 678, 1097, 1350, 1589
BstAFI CTTAAG 1 cut(s) 1784
BstAUI TGTACA 1 cut(s) 1216
BstBAI YACGTR 1 cut(s) 795
BstC8I GCNNGC 4 cut(s) 496, 606, 1191, 2364
BstDEI CTNAG 9 cut(s) 16, 474, 788, 876, 1159, 1434, 1865, 2184, 2196
BstENI CCTNNNNNAGG 1 cut(s) 875
BstF5I GGATG 4 cut(s) 166, 426, 553, 1699
BstH2I RGCGCY 2 cut(s) 1284, 1873
BstHHI GCGC 3 cut(s) 148, 1283, 1872
BstMAI GTCTC 5 cut(s) 156, 305, 475, 1111, 1173
BstMWI GCNNNNNNNGC 4 cut(s) 1697, 1844, 2008, 2466
BstNI CCWGG 2 cut(s) 57, 951
BstNSI RCATGY 1 cut(s) 1193
BstSCI CCNGG 3 cut(s) 55, 154, 949
BstSFI CTRYAG 5 cut(s) 402, 1101, 1337, 2364, 2373
BstV1I GCAGC 8 cut(s) 31, 376, 503, 1554, 1557, 1762, 2378, 2478
BstX2I RGATCY 4 cut(s) 631, 1030, 1598, 2437
BstXI CCANNNNNNTGG 1 cut(s) 1238
BstYI RGATCY 4 cut(s) 631, 1030, 1598, 2437
BstZ17I GTATAC 1 cut(s) 1917
BsuRI GGCC 6 cut(s) 276, 916, 949, 999, 1838, 2016
BtrI CACGTC 1 cut(s) 1183
BtsCI GGATG 4 cut(s) 166, 426, 553, 1699
BtsI GCAGTG 2 cut(s) 201, 1579
BtsIMutI CAGTG 5 cut(s) 201, 1318, 1466, 1579, 2364
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 4 cut(s) 496, 606, 1191, 2364
CaiI CAGNNNCTG 4 cut(s) 1109, 2134, 2363, 2372
CfoI GCGC 3 cut(s) 148, 1283, 1872
Cfr10I RCCGGY 1 cut(s) 764
Cfr13I GGNCC 4 cut(s) 1073, 1121, 1241, 1463
CseI GACGC 2 cut(s) 238, 2108
Csp6I GTAC 4 cut(s) 900, 1217, 1412, 2408
CspCI CAANNNNNGTGG 2 cut(s) 1366, 1401
CviQI GTAC 4 cut(s) 900, 1217, 1412, 2408
DdeI CTNAG 9 cut(s) 16, 474, 788, 876, 1159, 1434, 1865, 2184, 2196
DraI TTTAAA 1 cut(s) 460
DrdI GACNNNNNNGTC 1 cut(s) 1103
DseDI GACNNNNNNGTC 1 cut(s) 1103
EaeI YGGCCR 3 cut(s) 274, 947, 1836
Eam1104I CTCTTC 4 cut(s) 678, 1097, 1350, 1589
EarI CTCTTC 4 cut(s) 678, 1097, 1350, 1589
Eco130I CCWWGG 2 cut(s) 2020, 2461
Eco24I GRGCYC 1 cut(s) 1884
Eco47I GGWCC 4 cut(s) 1073, 1121, 1241, 1463
Eco57I CTGAAG 3 cut(s) 96, 505, 1608
Eco72I CACGTG 1 cut(s) 795
EcoNI CCTNNNNNAGG 1 cut(s) 875
EcoRI GAATTC 2 cut(s) 211, 375
EcoRII CCWGG 2 cut(s) 55, 949
EcoT14I CCWWGG 2 cut(s) 2020, 2461
EcoT22I ATGCAT 2 cut(s) 896, 2109
EcoT38I GRGCYC 1 cut(s) 1884
ErhI CCWWGG 2 cut(s) 2020, 2461
Esp3I CGTCTC 1 cut(s) 1173
FalI AAGNNNNNCTT 2 cut(s) 2004, 2036
FaqI GGGAC 2 cut(s) 1107, 2366
FauI CCCGC 1 cut(s) 503
FauNDI CATATG 2 cut(s) 1117, 1945
FbaI TGATCA 1 cut(s) 2053
FblI GTMKAC 2 cut(s) 479, 1916
FokI GGATG 4 cut(s) 173, 433, 540, 1706
FriOI GRGCYC 1 cut(s) 1884
FspBI CTAG 6 cut(s) 221, 297, 1332, 1442, 1502, 1664
GlaI GCGC 3 cut(s) 147, 1282, 1871
GsaI CCCAGC 1 cut(s) 1876
HaeII RGCGCY 2 cut(s) 1284, 1873
HaeIII GGCC 6 cut(s) 276, 916, 949, 999, 1838, 2016
HapII CCGG 3 cut(s) 155, 765, 1244
HgaI GACGC 2 cut(s) 238, 2108
HhaI GCGC 3 cut(s) 148, 1283, 1872
Hin6I GCGC 3 cut(s) 146, 1281, 1870
HinP1I GCGC 3 cut(s) 146, 1281, 1870
HincII GTYRAC 4 cut(s) 735, 801, 966, 1156
HindII GTYRAC 4 cut(s) 735, 801, 966, 1156
HindIII AAGCTT 3 cut(s) 235, 654, 671
HpaI GTTAAC 1 cut(s) 966
HpaII CCGG 3 cut(s) 155, 765, 1244
HphI GGTGA 3 cut(s) 30, 779, 1433
Hpy188III TCNNGA 7 cut(s) 136, 823, 830, 1930, 2305, 2338, 2441
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 6 cut(s) 567, 827, 1288, 1990, 2073, 2335
HpyCH4III ACNGT 5 cut(s) 739, 958, 1921, 1981, 2359
HpyCH4IV ACGT 6 cut(s) 180, 794, 962, 1182, 1991, 2139
HpyF10VI GCNNNNNNNGC 4 cut(s) 1697, 1844, 2008, 2466
HpyF3I CTNAG 9 cut(s) 16, 474, 788, 876, 1159, 1434, 1865, 2184, 2196
HpySE526I ACGT 6 cut(s) 180, 794, 962, 1182, 1991, 2139
HspAI GCGC 3 cut(s) 146, 1281, 1870
KpnI GGTACC 1 cut(s) 903
Ksp22I TGATCA 1 cut(s) 2053
KspAI GTTAAC 1 cut(s) 966
LguI GCTCTTC 1 cut(s) 1350
LmnI GCTCC 4 cut(s) 709, 1879, 2008, 2344
Lsp1109I GCAGC 8 cut(s) 31, 376, 503, 1554, 1557, 1762, 2378, 2478
LweI GCATC 6 cut(s) 175, 263, 292, 481, 789, 1762
MaeI CTAG 6 cut(s) 221, 297, 1332, 1442, 1502, 1664
MaeII ACGT 6 cut(s) 180, 794, 962, 1182, 1991, 2139
MaeIII GTNAC 6 cut(s) 19, 119, 292, 958, 1362, 1799
MfeI CAATTG 1 cut(s) 1767
MflI RGATCY 4 cut(s) 631, 1030, 1598, 2437
MhlI GDGCHC 2 cut(s) 794, 1884
MlsI TGGCCA 3 cut(s) 276, 949, 1838
MluNI TGGCCA 3 cut(s) 276, 949, 1838
MlyI GAGTC 7 cut(s) 485, 579, 847, 1091, 1432, 2411, 2456
MmeI TCCRAC 2 cut(s) 891, 1051
Mox20I TGGCCA 3 cut(s) 276, 949, 1838
Mph1103I ATGCAT 2 cut(s) 896, 2109
MscI TGGCCA 3 cut(s) 276, 949, 1838
MslI CAYNNNNRTG 3 cut(s) 335, 1236, 1275
Msp20I TGGCCA 3 cut(s) 276, 949, 1838
MspCI CTTAAG 1 cut(s) 1784
MspI CCGG 3 cut(s) 155, 765, 1244
MspR9I CCNGG 3 cut(s) 57, 156, 951
MunI CAATTG 1 cut(s) 1767
MvaI CCWGG 2 cut(s) 57, 951
MwoI GCNNNNNNNGC 4 cut(s) 1697, 1844, 2008, 2466
NciI CCSGG 1 cut(s) 156
NdeI CATATG 2 cut(s) 1117, 1945
NlaIV GGNNCC 6 cut(s) 570, 705, 901, 1123, 1881, 2439
NmuCI GTSAC 1 cut(s) 292
NsiI ATGCAT 2 cut(s) 896, 2109
NspI RCATGY 1 cut(s) 1193
PaeI GCATGC 1 cut(s) 1193
PaqCI CACCTGC 1 cut(s) 49
PciSI GCTCTTC 1 cut(s) 1350
PfeI GAWTC 5 cut(s) 76, 826, 1016, 1678, 2131
PleI GAGTC 7 cut(s) 484, 579, 847, 1091, 1432, 2410, 2455
PmaCI CACGTG 1 cut(s) 795
PmlI CACGTG 1 cut(s) 795
PpsI GAGTC 7 cut(s) 484, 579, 847, 1091, 1432, 2410, 2455
Ppu21I YACGTR 1 cut(s) 795
PshBI ATTAAT 3 cut(s) 354, 1209, 1320
PsiI TTATAA 2 cut(s) 242, 596
Psp6I CCWGG 2 cut(s) 55, 949
PspCI CACGTG 1 cut(s) 795
PspFI CCCAGC 1 cut(s) 1872
PspGI CCWGG 2 cut(s) 55, 949
PspN4I GGNNCC 6 cut(s) 570, 705, 901, 1123, 1881, 2439
PspPI GGNCC 4 cut(s) 1073, 1121, 1241, 1463
PstI CTGCAG 2 cut(s) 1105, 2368
PstNI CAGNNNCTG 4 cut(s) 1109, 2134, 2363, 2372
PsuI RGATCY 4 cut(s) 631, 1030, 1598, 2437
RsaI GTAC 4 cut(s) 901, 1218, 1413, 2409
RsaNI GTAC 4 cut(s) 900, 1217, 1412, 2408
RseI CAYNNNNRTG 3 cut(s) 335, 1236, 1275
SapI GCTCTTC 1 cut(s) 1350
Sau96I GGNCC 4 cut(s) 1073, 1121, 1241, 1463
SchI GAGTC 7 cut(s) 485, 579, 847, 1091, 1432, 2411, 2456
ScrFI CCNGG 3 cut(s) 57, 156, 951
SduI GDGCHC 2 cut(s) 794, 1884
SfaNI GCATC 6 cut(s) 175, 263, 292, 481, 789, 1762
SfcI CTRYAG 5 cut(s) 402, 1101, 1337, 2364, 2373
SinI GGWCC 4 cut(s) 1073, 1121, 1241, 1463
SmiMI CAYNNNNRTG 3 cut(s) 335, 1236, 1275
SmlI CTYRAG 6 cut(s) 563, 821, 1506, 1758, 1784, 2297
SmoI CTYRAG 6 cut(s) 563, 821, 1506, 1758, 1784, 2297
SphI GCATGC 1 cut(s) 1193
SsiI CCGC 6 cut(s) 99, 130, 496, 913, 1691, 2017
SspMI CTAG 6 cut(s) 221, 297, 1332, 1442, 1502, 1664
StyD4I CCNGG 3 cut(s) 55, 154, 949
StyI CCWWGG 2 cut(s) 2020, 2461
TaaI ACNGT 5 cut(s) 739, 958, 1921, 1981, 2359
TaiI ACGT 6 cut(s) 183, 797, 965, 1185, 1994, 2142
TaqI TCGA 4 cut(s) 8, 856, 1095, 2277
TatI WGTACW 1 cut(s) 1216
TauI GCSGC 3 cut(s) 102, 916, 2019
TfiI GAWTC 5 cut(s) 76, 826, 1016, 1678, 2131
TscAI CASTG 5 cut(s) 201, 1318, 1473, 1579, 2364
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 9 cut(s) 44, 389, 516, 1542, 1545, 1775, 2366, 2466, 2469
Tsp45I GTSAC 1 cut(s) 292
TspGWI ACGGA 2 cut(s) 2039, 2395
TspRI CASTG 5 cut(s) 201, 1318, 1473, 1579, 2364
Vha464I CTTAAG 1 cut(s) 1784
VpaK11BI GGWCC 4 cut(s) 1073, 1121, 1241, 1463
VspI ATTAAT 3 cut(s) 354, 1209, 1320
XagI CCTNNNNNAGG 1 cut(s) 875
XapI RAATTY 7 cut(s) 211, 375, 885, 1148, 1933, 2252, 2333
XceI RCATGY 1 cut(s) 1193
XmiI GTMKAC 2 cut(s) 479, 1916
XspI CTAG 6 cut(s) 221, 297, 1332, 1442, 1502, 1664
Zsp2I ATGCAT 2 cut(s) 896, 2109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.