MD09G1011100.v1.1

Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. The transcription regulatory histone acetylation (HAT) complex SAGA is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex that specifically deubiquitinates histones. The SAGA complex is recruited to specific gene promoters by activators, where it is required for transcription. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015292
Physical Location & Seq
Reverse (-)
732621 .. 735330
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1011100.v1.1.491

Sequence Viewer

Length: 453 bp
ATGTTCGTTCATTCACCATCATCAATAATCCTGATCAATCAATCAATCTACCTTCCTCCTTCAAGTCGCAGAGTGTTCGACGGCCGAGATTTACTTGGTCTCCGATTCCTAAGACCATGTCAGAAATCTGTGAATCGTCCACCGACGCCGGATGTTGCAGAAAATCAAGAAAAAGAGCCCACTCGTCAAGAGCTTATAAACATCGAGTTGATTGAGAGCGGGGAAAAGGAGAGGTTAATGGAGCTTCTGAGGGAAAGGCTGATAGAGTGCGGGTGGAAGGATGAAATGAAAGCTCTTTGCAGGGCGTTTATAAAGAAAAAGGGGAGGAACAATGTTACAGTTGATGATCTTGTACATGTAATGACCCCAAAGGGCAGAGCCTCCGTCCCCGATTCCGTGAAGGCGGAGCTTTTGCAAAGAATTCGTACATTTCTCGTGTCAGCAGCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EnY2

Protein Analysis

151

Amino Acids

17.18

Weight (kDa)

9.33

Isoelectric Point (pI)

59.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EnY2 PF10163 65 - 145 3e-31 Transcription factor e(y)2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015292)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27100 AT3G27100
fragaria_vesca FvH4_6g53480 FvH4_6g53480
malus_domestica MD00G1139400.v1.1 MD09G1011100.v1.1
prunus_persica Prupe.3G305900_v2.0.a1 Prupe.3G305900_v2.0.a1
pyrus_communis pycom111g00940
rosa_chinensis RchiOBHm_Chr2g0175521
rosa_laevigata RLG00000022335
rosa_multiflora Rmu_sc0021955.1_g000007
rosa_roxburghii Rroxscaffold_2G00077240
rosa_rugosa Rorug02G0585800
rosa_samantha Rh2CG639000
rosa_wichuraiana Rw2G054510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 197, 311
AccBSI CCGCTC 1 cut(s) 219
AciI CCGC 3 cut(s) 219, 270, 404
AcoI YGGCCR 1 cut(s) 82
AcsI RAATTY 1 cut(s) 420
AcyI GRCGYC 1 cut(s) 146
AfaI GTAC 2 cut(s) 354, 427
AflIII ACRYGT 1 cut(s) 355
AgsI TTSAA 1 cut(s) 63
AluBI AGCT 5 cut(s) 193, 244, 293, 409, 446
AluI AGCT 5 cut(s) 193, 244, 293, 409, 446
Alw26I GTCTC 1 cut(s) 104
AoxI GGCC 1 cut(s) 82
ApeKI GCWGC 1 cut(s) 443
ApoI RAATTY 1 cut(s) 420
AsuHPI GGTGA 1 cut(s) 6
BanII GRGCYC 1 cut(s) 180
BauI CACGAG 1 cut(s) 434
BccI CCATC 1 cut(s) 25
BceAI ACGGC 1 cut(s) 97
BcgI CGANNNNNNTGC 4 cut(s) 58, 92, 404, 438
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 104
BisI GCNGC 1 cut(s) 444
BlsI GCNGC 1 cut(s) 445
BsaHI GRCGYC 1 cut(s) 146
BsaI GGTCTC 1 cut(s) 104
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
BseGI GGATG 2 cut(s) 157, 286
BseMII CTCAG 1 cut(s) 239
BseX3I CGGCCG 1 cut(s) 82
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 1 cut(s) 84
BsiEI CGRYCG 1 cut(s) 85
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 371
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 1 cut(s) 84
Bso31I GGTCTC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 180
Bsp1407I TGTACA 1 cut(s) 352
Bsp143I GATC 2 cut(s) 33, 346
BspACI CCGC 3 cut(s) 219, 270, 404
BspANI GGCC 1 cut(s) 84
BspCNI CTCAG 1 cut(s) 240
BspTNI GGTCTC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 219
BsrGI TGTACA 1 cut(s) 352
BssMI GATC 2 cut(s) 33, 346
BssNI GRCGYC 1 cut(s) 146
BssSI CACGAG 1 cut(s) 434
Bst2BI CACGAG 1 cut(s) 434
Bst4CI ACNGT 1 cut(s) 340
BstACI GRCGYC 1 cut(s) 146
BstAUI TGTACA 1 cut(s) 352
BstDEI CTNAG 2 cut(s) 110, 248
BstF5I GGATG 2 cut(s) 157, 286
BstKTI GATC 2 cut(s) 36, 349
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 2 cut(s) 33, 346
BstMCI CGRYCG 1 cut(s) 85
BstNSI RCATGY 1 cut(s) 359
BstZI CGGCCG 1 cut(s) 82
BsuRI GGCC 1 cut(s) 84
BtsCI GGATG 2 cut(s) 157, 286
CseI GACGC 1 cut(s) 154
Csp6I GTAC 2 cut(s) 353, 426
CviAII CATG 2 cut(s) 117, 356
CviJI RGCY 9 cut(s) 84, 178, 193, 244, 259, 293, 380, 409, 446
CviKI_1 RGCY 9 cut(s) 84, 178, 193, 244, 259, 293, 380, 409, 446
CviQI GTAC 2 cut(s) 353, 426
DdeI CTNAG 2 cut(s) 110, 248
DpnI GATC 2 cut(s) 35, 348
DpnII GATC 2 cut(s) 33, 346
EaeI YGGCCR 1 cut(s) 82
EagI CGGCCG 1 cut(s) 82
EciI GGCGGA 1 cut(s) 419
EclXI CGGCCG 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 180
Eco31I GGTCTC 1 cut(s) 104
Eco52I CGGCCG 1 cut(s) 82
EcoRI GAATTC 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 180
FaeI CATG 2 cut(s) 120, 359
FaiI YATR 4 cut(s) 118, 197, 311, 357
FaqI GGGAC 1 cut(s) 371
FatI CATG 2 cut(s) 116, 355
FauI CCCGC 2 cut(s) 212, 263
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 1 cut(s) 444
FokI GGATG 2 cut(s) 164, 293
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 1 cut(s) 444
GluI GCNGC 1 cut(s) 444
HaeIII GGCC 1 cut(s) 84
HapII CCGG 1 cut(s) 149
HgaI GACGC 1 cut(s) 154
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 2 cut(s) 120, 359
HinfI GANTC 3 cut(s) 105, 133, 392
HpaII CCGG 1 cut(s) 149
HphI GGTGA 1 cut(s) 6
Hpy166II GTNNAC 1 cut(s) 140
Hpy188I TCNGA 3 cut(s) 104, 123, 249
Hpy188III TCNNGA 3 cut(s) 31, 167, 188
Hpy8I GTNNAC 1 cut(s) 140
Hpy99I CGWCG 2 cut(s) 83, 148
HpyAV CCTTC 4 cut(s) 62, 69, 271, 394
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4V TGCA 3 cut(s) 158, 300, 415
HpyF3I CTNAG 2 cut(s) 110, 248
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 2 cut(s) 120, 359
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 2 cut(s) 33, 346
LmnI GCTCC 2 cut(s) 241, 406
LpnPI CCDG 3 cut(s) 44, 162, 286
MaeIII GTNAC 1 cut(s) 334
MalI GATC 2 cut(s) 35, 348
MbiI CCGCTC 1 cut(s) 219
MboI GATC 2 cut(s) 33, 346
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 1 cut(s) 420
MnlI CCTC 5 cut(s) 66, 225, 243, 318, 391
MseI TTAA 1 cut(s) 236
MspI CCGG 1 cut(s) 149
NdeII GATC 2 cut(s) 33, 346
NlaIII CATG 2 cut(s) 120, 359
NmeAIII GCCGAG 1 cut(s) 110
NspI RCATGY 1 cut(s) 359
PciI ACATGT 1 cut(s) 355
PfeI GAWTC 3 cut(s) 105, 133, 392
PflFI GACNNNGTC 1 cut(s) 117
PkrI GCNGC 1 cut(s) 445
PscI ACATGT 1 cut(s) 355
PsiI TTATAA 2 cut(s) 197, 311
PsyI GACNNNGTC 1 cut(s) 117
RsaI GTAC 2 cut(s) 354, 427
RsaNI GTAC 2 cut(s) 353, 426
SaqAI TTAA 1 cut(s) 236
SatI GCNGC 1 cut(s) 444
Sau3AI GATC 2 cut(s) 33, 346
SduI GDGCHC 1 cut(s) 180
SetI ASST 7 cut(s) 54, 195, 236, 246, 295, 411, 448
Sse9I AATT 1 cut(s) 420
SsiI CCGC 3 cut(s) 219, 270, 404
TaaI ACNGT 1 cut(s) 340
TaqI TCGA 2 cut(s) 78, 204
TasI AATT 1 cut(s) 420
TatI WGTACW 1 cut(s) 352
TfiI GAWTC 3 cut(s) 105, 133, 392
Tru1I TTAA 1 cut(s) 236
Tru9I TTAA 1 cut(s) 236
TseI GCWGC 1 cut(s) 443
TspDTI ATGAA 2 cut(s) 297, 302
TspGWI ACGGA 2 cut(s) 373, 385
Tth111I GACNNNGTC 1 cut(s) 117
XapI RAATTY 1 cut(s) 420
XceI RCATGY 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.