RLG00000022335

Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. The transcription regulatory histone acetylation (HAT) complex SAGA is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex that specifically deubiquitinates histones. The SAGA complex is recruited to specific gene promoters by activators, where it is required for transcription. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015292
Physical Location & Seq
Forward (+)
82665349 .. 82668303
2955 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022335

Sequence Viewer

Length: 372 bp
ATGTGGGACCGGGCAGTCCCCAATAGACTAAAAACCATGAGGAAATCGGTGAATCGTCAGTCGACGCCGGATGTTGAGGAGAATCAAGACAAGGAGCCCAGTTTCCAAGAGCTCATCAACATCGAGTTGATTGAGAGCGGTGAAAAGGAGCGGTTAATGGAGCTACTGAGGGAGAGGCTAATTGAGTGTGGGTGGAAGGATGAAATGAAAGCTCTTTGCAGGTCATTCATAAAGAAAAAAGGAAGAAACAATGTTACTGTGGATGACCTTGTACATGTAATCACCCCAAAGGGCAGAGCCTCCATTCCTGATTCCGTAAAGGCAGAGCTTTTGCAAAGGATTCGTACGTTCCTGATGTCAGCAGCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EnY2

Protein Analysis

124

Amino Acids

14.31

Weight (kDa)

9.0

Isoelectric Point (pI)

51.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EnY2 PF10163 38 - 118 9.1e-32 Transcription factor e(y)2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015292)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27100 AT3G27100
fragaria_vesca FvH4_6g53480 FvH4_6g53480
malus_domestica MD00G1139400.v1.1 MD09G1011100.v1.1
prunus_persica Prupe.3G305900_v2.0.a1 Prupe.3G305900_v2.0.a1
pyrus_communis pycom111g00940
rosa_chinensis RchiOBHm_Chr2g0175521
rosa_laevigata RLG00000022335
rosa_multiflora Rmu_sc0021955.1_g000007
rosa_roxburghii Rroxscaffold_2G00077240
rosa_rugosa Rorug02G0585800
rosa_samantha Rh2CG639000
rosa_wichuraiana Rw2G054510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 210
AccBSI CCGCTC 2 cut(s) 138, 151
AccI GTMKAC 1 cut(s) 62
AciI CCGC 2 cut(s) 138, 151
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 2 cut(s) 273, 346
AflIII ACRYGT 1 cut(s) 274
AluBI AGCT 5 cut(s) 112, 163, 212, 328, 365
AluI AGCT 5 cut(s) 112, 163, 212, 328, 365
Alw21I GWGCWC 1 cut(s) 114
ApeKI GCWGC 1 cut(s) 362
AspS9I GGNCC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 3 cut(s) 61, 152, 274
AvaII GGWCC 1 cut(s) 7
BanII GRGCYC 2 cut(s) 99, 114
Bbv12I GWGCWC 1 cut(s) 114
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BcnI CCSGG 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 210
BisI GCNGC 1 cut(s) 363
BlsI GCNGC 1 cut(s) 364
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 2 cut(s) 8, 96
BmrFI CCNGG 1 cut(s) 11
BmrI ACTGGG 1 cut(s) 93
BmuI ACTGGG 1 cut(s) 93
BpuMI CCSGG 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 65
Bse1I ACTGG 1 cut(s) 99
BseGI GGATG 3 cut(s) 76, 205, 268
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 99
BseRI GAGGAG 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 114
BsiSI CCGG 2 cut(s) 10, 68
BsiWI CGTACG 1 cut(s) 344
BslFI GGGAC 2 cut(s) 2, 20
BsmFI GGGAC 2 cut(s) 2, 20
Bsp1286I GDGCHC 2 cut(s) 99, 114
Bsp1407I TGTACA 1 cut(s) 271
BspACI CCGC 2 cut(s) 138, 151
BspCNI CTCAG 1 cut(s) 159
BspLI GGNNCC 2 cut(s) 8, 96
BspMI ACCTGC 1 cut(s) 210
BsrBI CCGCTC 2 cut(s) 138, 151
BsrGI TGTACA 1 cut(s) 271
BsrI ACTGG 1 cut(s) 99
BssNI GRCGYC 1 cut(s) 65
Bst4CI ACNGT 1 cut(s) 259
BstACI GRCGYC 1 cut(s) 65
BstAUI TGTACA 1 cut(s) 271
BstDEI CTNAG 1 cut(s) 167
BstF5I GGATG 3 cut(s) 76, 205, 268
BstNSI RCATGY 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 9
BtsCI GGATG 3 cut(s) 76, 205, 268
BveI ACCTGC 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 7
CseI GACGC 1 cut(s) 73
Csp6I GTAC 2 cut(s) 272, 345
CviAII CATG 2 cut(s) 37, 275
CviJI RGCY 8 cut(s) 97, 112, 163, 178, 212, 299, 328, 365
CviKI_1 RGCY 8 cut(s) 97, 112, 163, 178, 212, 299, 328, 365
CviQI GTAC 2 cut(s) 272, 345
DdeI CTNAG 1 cut(s) 167
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
Ecl136II GAGCTC 1 cut(s) 112
Eco24I GRGCYC 2 cut(s) 99, 114
Eco47I GGWCC 1 cut(s) 7
Eco53kI GAGCTC 1 cut(s) 112
EcoICRI GAGCTC 1 cut(s) 112
EcoT38I GRGCYC 2 cut(s) 99, 114
FaeI CATG 2 cut(s) 40, 278
FaiI YATR 3 cut(s) 38, 230, 276
FaqI GGGAC 2 cut(s) 2, 20
FatI CATG 2 cut(s) 36, 274
FblI GTMKAC 1 cut(s) 62
Fnu4HI GCNGC 1 cut(s) 363
FokI GGATG 3 cut(s) 83, 212, 275
FriOI GRGCYC 2 cut(s) 99, 114
Fsp4HI GCNGC 1 cut(s) 363
GluI GCNGC 1 cut(s) 363
HapII CCGG 2 cut(s) 10, 68
HgaI GACGC 1 cut(s) 73
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 2 cut(s) 40, 278
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HinfI GANTC 4 cut(s) 52, 82, 311, 340
HpaII CCGG 2 cut(s) 10, 68
HphI GGTGA 3 cut(s) 61, 152, 274
Hpy166II GTNNAC 1 cut(s) 63
Hpy188III TCNNGA 3 cut(s) 86, 308, 352
Hpy8I GTNNAC 1 cut(s) 63
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4IV ACGT 1 cut(s) 347
HpyCH4V TGCA 2 cut(s) 219, 334
HpyF3I CTNAG 1 cut(s) 167
HpySE526I ACGT 1 cut(s) 347
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 2 cut(s) 40, 278
LmnI GCTCC 3 cut(s) 94, 148, 160
LpnPI CCDG 6 cut(s) 23, 81, 112, 205, 321, 365
MaeII ACGT 1 cut(s) 347
MaeIII GTNAC 1 cut(s) 253
MbiI CCGCTC 2 cut(s) 138, 151
MboII GAAGA 1 cut(s) 255
MhlI GDGCHC 2 cut(s) 99, 114
MluCI AATT 1 cut(s) 180
MnlI CCTC 5 cut(s) 33, 70, 162, 168, 310
MseI TTAA 2 cut(s) 155, 370
MspI CCGG 2 cut(s) 10, 68
MspR9I CCNGG 1 cut(s) 11
NciI CCSGG 1 cut(s) 11
NlaIII CATG 2 cut(s) 40, 278
NlaIV GGNNCC 2 cut(s) 8, 96
NspI RCATGY 1 cut(s) 278
PciI ACATGT 1 cut(s) 274
PfeI GAWTC 4 cut(s) 52, 82, 311, 340
Pfl23II CGTACG 1 cut(s) 344
PkrI GCNGC 1 cut(s) 364
PscI ACATGT 1 cut(s) 274
Psp124BI GAGCTC 1 cut(s) 114
PspLI CGTACG 1 cut(s) 344
PspN4I GGNNCC 2 cut(s) 8, 96
PspPI GGNCC 1 cut(s) 7
RsaI GTAC 2 cut(s) 273, 346
RsaNI GTAC 2 cut(s) 272, 345
SacI GAGCTC 1 cut(s) 114
SalI GTCGAC 1 cut(s) 61
SaqAI TTAA 2 cut(s) 155, 370
SatI GCNGC 1 cut(s) 363
Sau96I GGNCC 1 cut(s) 7
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 2 cut(s) 99, 114
SetI ASST 8 cut(s) 114, 165, 214, 224, 270, 330, 350, 367
SinI GGWCC 1 cut(s) 7
Sse9I AATT 1 cut(s) 180
SsiI CCGC 2 cut(s) 138, 151
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 259
TaiI ACGT 1 cut(s) 350
TaqI TCGA 2 cut(s) 62, 123
TasI AATT 1 cut(s) 180
TatI WGTACW 1 cut(s) 271
TfiI GAWTC 4 cut(s) 52, 82, 311, 340
Tru1I TTAA 2 cut(s) 155, 370
Tru9I TTAA 2 cut(s) 155, 370
TseI GCWGC 1 cut(s) 362
TspDTI ATGAA 3 cut(s) 216, 217, 221
TspGWI ACGGA 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 7
XceI RCATGY 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.