MD09G1019300.v1.1

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013295
Physical Location & Seq
Reverse (-)
1171447 .. 1172528
1082 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1019300.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGCTAACTTCAACTGCAACACGATCTTTCTGTTTTCTCTGGTGATGTTTTTCTTCTGCTATGGTCATTTCGTCTCCGCCTTCGAGTTCCAAGTTGGTGGAAACAAAGACTGGGTTGTCCCTCCCGCCAACGACACCAAAATCTACAACGATTGGGCCTCTGAAAACCGGTTCCAAGTCGGCGACACCCTCCGCTTTCACTACAAGAAGGACTCGGTGATGGAGGTGACAGAGGCGGAGTACAAAAAGTGCAACTCGACTCGCCCCAACTTCTTCTCGAACACTGGAAACACCGTTTACACGTTCGACCACTCGGGCTCCTTCTACTTCATCAGTGGAGCCGCTGGACACTGCGAGAGGGGACAGCGCATGATAGTTAAGGTGATGGCGGCGGAGGATGAGGACTCTCCGTCGGGTGGTGACGCCATAAAATCTTCCGGTGCTGCGGTTTCCTCAGTCGGAGTCTCCAAACTTGTCCTTGTTCAGTTTGTTATGTCGGTTGTTAATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

171

Amino Acids

18.94

Weight (kDa)

5.76

Isoelectric Point (pI)

22.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 47 - 122 7.3e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14345
fragaria_vesca FvH4_6g52240
malus_domestica MD09G1019300.v1.1 MD17G1019900.v1.1
prunus_persica Prupe.3G297100_v2.0.a1 Prupe.3G297700_v2.0.a1
pyrus_communis pycom111g01600 pycom17g01650
rosa_chinensis RchiOBHm_Chr2g0173791
rosa_laevigata RLG00000022222
rosa_multiflora Rmu_sc0033569.1_g000002
rosa_roxburghii Rroxscaffold_2G00078620
rosa_rugosa Rorug02G0572800
rosa_samantha Rh2AG651800 Rh2BG662500 Rh2CG627200 Rh2DG677600
rosa_wichuraiana Rw2G053510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AciI CCGC 8 cut(s) 78, 126, 193, 236, 342, 389, 392, 446
AcyI GRCGYC 1 cut(s) 423
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 1 cut(s) 416
AflIII ACRYGT 1 cut(s) 300
AgeI ACCGGT 1 cut(s) 168
AgsI TTSAA 1 cut(s) 13
AhdI GACNNNNNGTC 1 cut(s) 409
Alw26I GTCTC 2 cut(s) 79, 469
Ama87I CYCGRG 1 cut(s) 313
AoxI GGCC 1 cut(s) 156
ApeKI GCWGC 1 cut(s) 443
AsiGI ACCGGT 1 cut(s) 168
AspLEI GCGC 1 cut(s) 369
AspS9I GGNCC 1 cut(s) 156
AsuHPI GGTGA 5 cut(s) 55, 229, 238, 394, 431
AvaI CYCGRG 1 cut(s) 313
BanII GRGCYC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 430
BccI CCATC 2 cut(s) 214, 379
BcoDI GTCTC 2 cut(s) 79, 469
BfaI CTAG 1 cut(s) 511
BisI GCNGC 3 cut(s) 342, 390, 444
BlsI GCNGC 3 cut(s) 343, 391, 445
BmeRI GACNNNNNGTC 1 cut(s) 409
BmeT110I CYCGRG 1 cut(s) 313
BmgT120I GGNCC 1 cut(s) 156
BmiI GGNNCC 3 cut(s) 173, 319, 340
BmrI ACTGGG 1 cut(s) 121
BmuI ACTGGG 1 cut(s) 121
BsaHI GRCGYC 1 cut(s) 423
BsaWI WCCGGW 2 cut(s) 168, 437
BsaXI ACNNNNNCTCC 2 cut(s) 302, 332
Bsc4I CCNNNNNNNGG 1 cut(s) 416
Bse118I RCCGGY 1 cut(s) 168
Bse1I ACTGG 2 cut(s) 116, 289
BseGI GGATG 1 cut(s) 403
BseLI CCNNNNNNNGG 1 cut(s) 416
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 2 cut(s) 116, 289
BseXI GCAGC 1 cut(s) 430
BshFI GGCC 1 cut(s) 158
BshTI ACCGGT 1 cut(s) 168
BsiHKCI CYCGRG 1 cut(s) 313
BsiSI CCGG 2 cut(s) 169, 438
BslFI GGGAC 2 cut(s) 104, 375
BslI CCNNNNNNNGG 1 cut(s) 416
BsmAI GTCTC 2 cut(s) 79, 469
BsmBI CGTCTC 1 cut(s) 79
BsmFI GGGAC 2 cut(s) 104, 375
BsnI GGCC 1 cut(s) 158
BsoBI CYCGRG 1 cut(s) 313
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 1 cut(s) 24
BspACI CCGC 8 cut(s) 78, 126, 193, 236, 342, 389, 392, 446
BspANI GGCC 1 cut(s) 158
BspCNI CTCAG 1 cut(s) 467
BspLI GGNNCC 3 cut(s) 173, 319, 340
BsrFI RCCGGY 1 cut(s) 168
BsrI ACTGG 2 cut(s) 116, 289
BssAI RCCGGY 1 cut(s) 168
BssMI GATC 1 cut(s) 24
BssNI GRCGYC 1 cut(s) 423
Bst4CI ACNGT 1 cut(s) 295
BstACI GRCGYC 1 cut(s) 423
BstDEI CTNAG 1 cut(s) 454
BstF5I GGATG 1 cut(s) 403
BstHHI GCGC 1 cut(s) 369
BstKTI GATC 1 cut(s) 27
BstMAI GTCTC 2 cut(s) 79, 469
BstMBI GATC 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 430
BstXI CCANNNNNNTGG 1 cut(s) 98
BsuRI GGCC 1 cut(s) 158
BtsCI GGATG 1 cut(s) 403
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 3 cut(s) 282, 340, 349
CfoI GCGC 1 cut(s) 369
Cfr10I RCCGGY 1 cut(s) 168
Cfr13I GGNCC 1 cut(s) 156
CseI GACGC 1 cut(s) 431
Csp6I GTAC 1 cut(s) 241
CspAI ACCGGT 1 cut(s) 168
CviAII CATG 1 cut(s) 370
CviJI RGCY 4 cut(s) 5, 158, 318, 341
CviKI_1 RGCY 4 cut(s) 5, 158, 318, 341
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 1 cut(s) 454
DpnI GATC 1 cut(s) 26
DpnII GATC 1 cut(s) 24
DrdI GACNNNNNNGTC 1 cut(s) 116
DriI GACNNNNNGTC 1 cut(s) 409
DseDI GACNNNNNNGTC 1 cut(s) 116
Eam1105I GACNNNNNGTC 1 cut(s) 409
EciI GGCGGA 3 cut(s) 67, 251, 407
Eco24I GRGCYC 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 313
EcoT38I GRGCYC 1 cut(s) 320
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 1 cut(s) 373
FaiI YATR 4 cut(s) 63, 371, 428, 494
FaqI GGGAC 2 cut(s) 104, 375
FatI CATG 1 cut(s) 369
FauI CCCGC 1 cut(s) 133
Fnu4HI GCNGC 3 cut(s) 342, 390, 444
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 1 cut(s) 320
Fsp4HI GCNGC 3 cut(s) 342, 390, 444
FspBI CTAG 1 cut(s) 511
GlaI GCGC 1 cut(s) 368
GluI GCNGC 3 cut(s) 342, 390, 444
HaeIII GGCC 1 cut(s) 158
HapII CCGG 2 cut(s) 169, 438
HgaI GACGC 1 cut(s) 431
HhaI GCGC 1 cut(s) 369
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 1 cut(s) 373
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HinfI GANTC 4 cut(s) 212, 259, 404, 462
HpaII CCGG 2 cut(s) 169, 438
HphI GGTGA 5 cut(s) 55, 229, 238, 394, 431
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 2 cut(s) 163, 461
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 1 cut(s) 298
Hpy99I CGWCG 1 cut(s) 415
HpyAV CCTTC 3 cut(s) 91, 202, 331
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 2 cut(s) 18, 252
HpyF3I CTNAG 1 cut(s) 454
HpySE526I ACGT 1 cut(s) 302
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 1 cut(s) 373
HspAI GCGC 1 cut(s) 367
Kzo9I GATC 1 cut(s) 24
LmnI GCTCC 2 cut(s) 323, 338
LpnPI CCDG 6 cut(s) 26, 97, 182, 270, 330, 451
Lsp1109I GCAGC 1 cut(s) 430
MaeI CTAG 1 cut(s) 511
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 2 cut(s) 226, 419
MalI GATC 1 cut(s) 26
MboI GATC 1 cut(s) 24
MboII GAAGA 3 cut(s) 46, 265, 426
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 1 cut(s) 505
MlyI GAGTC 4 cut(s) 206, 253, 398, 471
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 9 cut(s) 132, 169, 200, 217, 226, 351, 388, 394, 463
MseI TTAA 2 cut(s) 378, 504
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 2 cut(s) 169, 438
NdeII GATC 1 cut(s) 24
NlaIII CATG 1 cut(s) 373
NlaIV GGNNCC 3 cut(s) 173, 319, 340
NmuCI GTSAC 2 cut(s) 226, 419
PinAI ACCGGT 1 cut(s) 168
PkrI GCNGC 3 cut(s) 343, 391, 445
PleI GAGTC 4 cut(s) 206, 253, 398, 470
PpsI GAGTC 4 cut(s) 206, 253, 398, 470
PspN4I GGNNCC 3 cut(s) 173, 319, 340
PspPI GGNCC 1 cut(s) 156
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
SaqAI TTAA 2 cut(s) 378, 504
SatI GCNGC 3 cut(s) 342, 390, 444
Sau3AI GATC 1 cut(s) 24
Sau96I GGNCC 1 cut(s) 156
SchI GAGTC 4 cut(s) 206, 253, 398, 471
SduI GDGCHC 1 cut(s) 320
SetI ASST 3 cut(s) 228, 305, 384
Sse9I AATT 1 cut(s) 505
SsiI CCGC 8 cut(s) 78, 126, 193, 236, 342, 389, 392, 446
SspMI CTAG 1 cut(s) 511
TaaI ACNGT 1 cut(s) 295
TaiI ACGT 1 cut(s) 305
TaqI TCGA 4 cut(s) 84, 257, 278, 306
TasI AATT 1 cut(s) 505
TatI WGTACW 1 cut(s) 240
TauI GCSGC 2 cut(s) 344, 392
Tru1I TTAA 2 cut(s) 378, 504
Tru9I TTAA 2 cut(s) 378, 504
TscAI CASTG 3 cut(s) 289, 340, 356
TseFI GTSAC 2 cut(s) 226, 419
TseI GCWGC 1 cut(s) 443
Tsp45I GTSAC 2 cut(s) 226, 419
TspDTI ATGAA 1 cut(s) 319
TspGWI ACGGA 1 cut(s) 399
TspRI CASTG 3 cut(s) 289, 340, 356
XspI CTAG 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.