MD17G1019900.v1.1

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013295
Physical Location & Seq
Reverse (-)
1511403 .. 1512234
832 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1019900.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGCTAACTTCAACTGCAACATGGTTTATCTGTTTTATCTGATGATGGTTTTCTTCTGGTTTGGTCATTTCTCGGCCTCCGCCTTCGAGTTCCAGGTTGGTGGAAACAAAGACTGGGTTGTCCCTCCTGCCAACGACACCAAAATCTACAACGACTGGGCCTCCGAAAACCGTTTCCAAGTCGGCGACACCATCCGCTTTCACTACAAGAAGGATTCGGTGATGGAGGTGACGGAGGCGGAGTACAAGAAGTGCAACTCGACTCGCCCCAACTTCTTCTCCAACACTGGAAACACCGTTTACACGTTGGACCACTCGGGCTCCTTCTACTTCATCAGTGGAGCCGCTGGACACTGCGAGCGGGGACAGCGCATGATAGTTAAGGTGATGGCGGCGGAGGAGGAGGACTTTCCGTCTGGTGGCGACGCCAAAAGATCTTCCGATGCTGCAGTTTCCTCAGTTGGAGTCTTCAAGCATGTTCTTGTTCCGTTTGTTATGTCATATGCTGTTGCTTCTGTTATGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

176

Amino Acids

19.76

Weight (kDa)

5.92

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 48 - 123 2.8e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14345
fragaria_vesca FvH4_6g52240
malus_domestica MD09G1019300.v1.1 MD17G1019900.v1.1
prunus_persica Prupe.3G297100_v2.0.a1 Prupe.3G297700_v2.0.a1
pyrus_communis pycom111g01600 pycom17g01650
rosa_chinensis RchiOBHm_Chr2g0173791
rosa_laevigata RLG00000022222
rosa_multiflora Rmu_sc0033569.1_g000002
rosa_roxburghii Rroxscaffold_2G00078620
rosa_rugosa Rorug02G0572800
rosa_samantha Rh2AG651800 Rh2BG662500 Rh2CG627200 Rh2DG677600
rosa_wichuraiana Rw2G053510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 361
AciI CCGC 7 cut(s) 81, 196, 239, 345, 361, 392, 395
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 1 cut(s) 419
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 2 cut(s) 13, 472
AhdI GACNNNNNGTC 1 cut(s) 412
AjnI CCWGG 1 cut(s) 93
Ama87I CYCGRG 1 cut(s) 316
AoxI GGCC 2 cut(s) 75, 159
ApeKI GCWGC 1 cut(s) 446
AspLEI GCGC 1 cut(s) 372
AspS9I GGNCC 2 cut(s) 159, 310
AsuHPI GGTGA 3 cut(s) 232, 241, 397
AvaI CYCGRG 1 cut(s) 316
AvaII GGWCC 1 cut(s) 310
BanII GRGCYC 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 460
BbvI GCAGC 1 cut(s) 433
BccI CCATC 4 cut(s) 40, 200, 217, 382
BciT130I CCWGG 1 cut(s) 95
BfaI CTAG 1 cut(s) 526
BfmI CTRYAG 1 cut(s) 447
BglII AGATCT 1 cut(s) 434
BisI GCNGC 3 cut(s) 345, 393, 447
BlsI GCNGC 3 cut(s) 346, 394, 448
Bme1390I CCNGG 1 cut(s) 95
Bme18I GGWCC 1 cut(s) 310
BmeRI GACNNNNNGTC 1 cut(s) 412
BmeT110I CYCGRG 1 cut(s) 316
BmgT120I GGNCC 2 cut(s) 159, 310
BmiI GGNNCC 2 cut(s) 322, 343
BmrFI CCNGG 1 cut(s) 95
BmrI ACTGGG 2 cut(s) 124, 166
BmsI GCATC 1 cut(s) 433
BmuI ACTGGG 2 cut(s) 124, 166
BpiI GAAGAC 1 cut(s) 460
BsaHI GRCGYC 1 cut(s) 426
BsaXI ACNNNNNCTCC 8 cut(s) 146, 176, 227, 257, 263, 293, 305, 335
Bsc4I CCNNNNNNNGG 1 cut(s) 419
Bse1I ACTGG 3 cut(s) 119, 161, 292
BseBI CCWGG 1 cut(s) 95
BseGI GGATG 1 cut(s) 192
BseLI CCNNNNNNNGG 1 cut(s) 419
BseMII CTCAG 1 cut(s) 471
BseNI ACTGG 3 cut(s) 119, 161, 292
BseRI GAGGAG 2 cut(s) 413, 416
BseXI GCAGC 1 cut(s) 433
BshFI GGCC 2 cut(s) 77, 161
BsiHKCI CYCGRG 1 cut(s) 316
BslFI GGGAC 2 cut(s) 107, 378
BslI CCNNNNNNNGG 1 cut(s) 419
BsmFI GGGAC 2 cut(s) 107, 378
BsnI GGCC 2 cut(s) 77, 161
BsoBI CYCGRG 1 cut(s) 316
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 1 cut(s) 434
BspACI CCGC 7 cut(s) 81, 196, 239, 345, 361, 392, 395
BspANI GGCC 2 cut(s) 77, 161
BspCNI CTCAG 1 cut(s) 470
BspLI GGNNCC 2 cut(s) 322, 343
BspMAI CTGCAG 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 361
BsrI ACTGG 3 cut(s) 119, 161, 292
BssMI GATC 1 cut(s) 434
BssNI GRCGYC 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 2 cut(s) 173, 298
BstACI GRCGYC 1 cut(s) 426
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 1 cut(s) 457
BstF5I GGATG 1 cut(s) 192
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 1 cut(s) 437
BstMBI GATC 1 cut(s) 434
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNI CCWGG 1 cut(s) 95
BstNSI RCATGY 1 cut(s) 479
BstSCI CCNGG 1 cut(s) 93
BstSFI CTRYAG 1 cut(s) 447
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 1 cut(s) 460
BstX2I RGATCY 1 cut(s) 434
BstXI CCANNNNNNTGG 1 cut(s) 101
BstYI RGATCY 1 cut(s) 434
BsuRI GGCC 2 cut(s) 77, 161
BtsCI GGATG 1 cut(s) 192
BtsI GCAGTG 1 cut(s) 352
BtsIMutI CAGTG 3 cut(s) 285, 343, 352
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 372
Cfr13I GGNCC 2 cut(s) 159, 310
CseI GACGC 1 cut(s) 434
Csp6I GTAC 1 cut(s) 244
CviAII CATG 3 cut(s) 22, 373, 476
CviJI RGCY 5 cut(s) 5, 77, 161, 321, 344
CviKI_1 RGCY 5 cut(s) 5, 77, 161, 321, 344
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 1 cut(s) 457
DpnI GATC 1 cut(s) 436
DpnII GATC 1 cut(s) 434
DrdI GACNNNNNNGTC 1 cut(s) 119
DriI GACNNNNNGTC 1 cut(s) 412
DseDI GACNNNNNNGTC 1 cut(s) 119
Eam1105I GACNNNNNGTC 1 cut(s) 412
EciI GGCGGA 3 cut(s) 70, 254, 410
Eco24I GRGCYC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 323
FaeI CATG 3 cut(s) 25, 376, 479
FaiI YATR 7 cut(s) 23, 374, 477, 497, 502, 504, 521
FaqI GGGAC 2 cut(s) 107, 378
FatI CATG 3 cut(s) 21, 372, 475
FauI CCCGC 1 cut(s) 354
FauNDI CATATG 1 cut(s) 502
Fnu4HI GCNGC 3 cut(s) 345, 393, 447
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 3 cut(s) 345, 393, 447
FspBI CTAG 1 cut(s) 526
GlaI GCGC 1 cut(s) 371
GluI GCNGC 3 cut(s) 345, 393, 447
HaeIII GGCC 2 cut(s) 77, 161
HgaI GACGC 1 cut(s) 434
HhaI GCGC 1 cut(s) 372
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 3 cut(s) 25, 376, 479
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HinfI GANTC 3 cut(s) 215, 262, 465
HphI GGTGA 3 cut(s) 232, 241, 397
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 3 cut(s) 42, 166, 442
Hpy8I GTNNAC 1 cut(s) 301
Hpy99I CGWCG 1 cut(s) 428
HpyAV CCTTC 3 cut(s) 94, 205, 334
HpyCH4III ACNGT 2 cut(s) 173, 298
HpyCH4IV ACGT 1 cut(s) 305
HpyCH4V TGCA 3 cut(s) 18, 255, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpyF3I CTNAG 1 cut(s) 457
HpySE526I ACGT 1 cut(s) 305
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 3 cut(s) 25, 376, 479
HspAI GCGC 1 cut(s) 370
Kzo9I GATC 1 cut(s) 434
LmnI GCTCC 2 cut(s) 326, 341
LpnPI CCDG 9 cut(s) 43, 80, 100, 107, 141, 142, 273, 333, 402
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 1 cut(s) 433
MaeI CTAG 1 cut(s) 526
MaeII ACGT 1 cut(s) 305
MaeIII GTNAC 1 cut(s) 229
MalI GATC 1 cut(s) 436
MbiI CCGCTC 1 cut(s) 361
MboI GATC 1 cut(s) 434
MboII GAAGA 4 cut(s) 46, 268, 429, 460
MflI RGATCY 1 cut(s) 434
MhlI GDGCHC 1 cut(s) 323
MlyI GAGTC 2 cut(s) 256, 474
MmeI TCCRAC 3 cut(s) 288, 306, 442
MnlI CCTC 9 cut(s) 88, 135, 172, 220, 229, 391, 394, 397, 466
MseI TTAA 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 347
MspR9I CCNGG 1 cut(s) 95
MvaI CCWGG 1 cut(s) 95
MwoI GCNNNNNNNGC 1 cut(s) 367
NdeI CATATG 1 cut(s) 502
NdeII GATC 1 cut(s) 434
NlaIII CATG 3 cut(s) 25, 376, 479
NlaIV GGNNCC 2 cut(s) 322, 343
NmeAIII GCCGAG 1 cut(s) 53
NmuCI GTSAC 1 cut(s) 229
NspI RCATGY 1 cut(s) 479
PfeI GAWTC 1 cut(s) 215
PkrI GCNGC 3 cut(s) 346, 394, 448
PleI GAGTC 2 cut(s) 256, 473
PpsI GAGTC 2 cut(s) 256, 473
Psp6I CCWGG 1 cut(s) 93
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 2 cut(s) 322, 343
PspPI GGNCC 2 cut(s) 159, 310
PstI CTGCAG 1 cut(s) 451
PsuI RGATCY 1 cut(s) 434
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 3 cut(s) 345, 393, 447
Sau3AI GATC 1 cut(s) 434
Sau96I GGNCC 2 cut(s) 159, 310
SchI GAGTC 2 cut(s) 256, 474
ScrFI CCNGG 1 cut(s) 95
SduI GDGCHC 1 cut(s) 323
SetI ASST 4 cut(s) 99, 231, 308, 387
SfaNI GCATC 1 cut(s) 433
SfcI CTRYAG 1 cut(s) 447
SinI GGWCC 1 cut(s) 310
SsiI CCGC 7 cut(s) 81, 196, 239, 345, 361, 392, 395
SspMI CTAG 1 cut(s) 526
StyD4I CCNGG 1 cut(s) 93
TaaI ACNGT 2 cut(s) 173, 298
TaiI ACGT 1 cut(s) 308
TaqI TCGA 2 cut(s) 87, 260
TatI WGTACW 1 cut(s) 243
TauI GCSGC 2 cut(s) 347, 395
TfiI GAWTC 1 cut(s) 215
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 3 cut(s) 292, 343, 359
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 322
TspGWI ACGGA 3 cut(s) 248, 402, 477
TspRI CASTG 3 cut(s) 292, 343, 359
VpaK11BI GGWCC 1 cut(s) 310
XceI RCATGY 1 cut(s) 479
XspI CTAG 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.