Rh2CG627200

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0013295
Physical Location & Seq
Forward (+)
80116697 .. 80117496
800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG627200.1

Sequence Viewer

Length: 702 bp
ATGGGTCACGTCGCCTCCCGAGTCCCCACTATCTCAGTTAGTGTAAGTAACTACCTCTTCCACAACTTCAACCCCTCTCTATATATTAACCCTCGTAAACACTCCCATAATTACATTTCACCTAGTCTTCTTCTAGCTTTGCATCATTCTGAGATTGAGAGAGAGCTAACTCTGAGACTGAGAGACCCTCCTATGGCTGCTTTTAAAACAAGTTTGTTCTGGCTCGTCATTTTCATCTCTCTTGGGAACTCGTTGGTTTTCTCAGCTTTTGAGTTCCAGGTTGGTGGAACTCAAGGTTGGGTCGTCCCTCCAACCAATGACAGCAAGGTCTACAACGACTGGGCTTCAGAAAACAGGTTCCAAATCGGCAACGCCATCCGTTTTAAGTACAAGAAGGACTCGGTGATGGAGGTGACGGAAGAGGACTACAAGAAGTGCAATTCAAGTCATCCCAACTTCTTCTCCAACAATGGAAACACTGTCTACAGGTTTGACCATTCGGGGTCGTTTTACTTCATCAGTGGGGCAGCCGGACACTGCGAAAAGGGGCAGAGGATGATCGTTAAGGTCATGTCGACCGATGAGGAGTCTCCGTTGGGCAAGAAATCTGCTGGCTATCGTGCTGCGGTTTACGAGCTTGGAGTGTCCAAACTTGTGTTTGTTCAGTTTGTTTTGTTCTGTGTTGCTTCTTTAGTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

233

Amino Acids

26.34

Weight (kDa)

8.9

Isoelectric Point (pI)

25.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 108 - 184 5.2e-19 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14345
fragaria_vesca FvH4_6g52240
malus_domestica MD09G1019300.v1.1 MD17G1019900.v1.1
prunus_persica Prupe.3G297100_v2.0.a1 Prupe.3G297700_v2.0.a1
pyrus_communis pycom111g01600 pycom17g01650
rosa_chinensis RchiOBHm_Chr2g0173791
rosa_laevigata RLG00000022222
rosa_multiflora Rmu_sc0033569.1_g000002
rosa_roxburghii Rroxscaffold_2G00078620
rosa_rugosa Rorug02G0572800
rosa_samantha Rh2AG651800 Rh2BG662500 Rh2CG627200 Rh2DG677600
rosa_wichuraiana Rw2G053510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 326
AccI GTMKAC 3 cut(s) 330, 483, 575
AciI CCGC 1 cut(s) 626
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 193
AgsI TTSAA 2 cut(s) 70, 444
AjiI CACGTC 1 cut(s) 10
AjnI CCWGG 1 cut(s) 276
AjuI GAANNNNNNNTTGG 2 cut(s) 280, 312
AluBI AGCT 4 cut(s) 137, 166, 266, 637
AluI AGCT 4 cut(s) 137, 166, 266, 637
Alw26I GTCTC 3 cut(s) 169, 177, 594
Ama87I CYCGRG 1 cut(s) 18
ApeKI GCWGC 3 cut(s) 197, 527, 623
AsuHPI GGTGA 3 cut(s) 111, 415, 424
AvaI CYCGRG 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 119
BbvI GCAGC 3 cut(s) 184, 539, 610
BccI CCATC 2 cut(s) 383, 400
BciT130I CCWGG 1 cut(s) 278
BcoDI GTCTC 3 cut(s) 169, 177, 594
BfaI CTAG 3 cut(s) 123, 134, 700
BfmI CTRYAG 1 cut(s) 484
BisI GCNGC 3 cut(s) 198, 528, 624
BlsI GCNGC 3 cut(s) 199, 529, 625
Bme1390I CCNGG 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 18
BmgBI CACGTC 1 cut(s) 10
BmiI GGNNCC 1 cut(s) 359
BmrFI CCNGG 1 cut(s) 278
BmrI ACTGGG 1 cut(s) 349
BmsI GCATC 1 cut(s) 151
BmuI ACTGGG 1 cut(s) 349
BpiI GAAGAC 1 cut(s) 119
BplI GAGNNNNNCTC 2 cut(s) 172, 204
BpuEI CTTGAG 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 177
BsaXI ACNNNNNCTCC 5 cut(s) 29, 446, 476, 578, 608
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 344
BseBI CCWGG 1 cut(s) 278
BseGI GGATG 3 cut(s) 375, 448, 561
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMII CTCAG 5 cut(s) 48, 141, 164, 170, 276
BseNI ACTGG 1 cut(s) 344
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 3 cut(s) 184, 539, 610
Bsh1285I CGRYCG 1 cut(s) 579
BsiEI CGRYCG 1 cut(s) 579
BsiHKCI CYCGRG 1 cut(s) 18
BsiSI CCGG 1 cut(s) 531
BslFI GGGAC 2 cut(s) 8, 290
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 3 cut(s) 169, 177, 594
BsmFI GGGAC 2 cut(s) 8, 290
Bso31I GGTCTC 1 cut(s) 177
BsoBI CYCGRG 1 cut(s) 18
Bsp143I GATC 1 cut(s) 558
BspACI CCGC 1 cut(s) 626
BspCNI CTCAG 5 cut(s) 47, 142, 165, 171, 275
BspLI GGNNCC 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 177
BsrI ACTGG 1 cut(s) 344
BssMI GATC 1 cut(s) 558
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 1 cut(s) 481
Bst6I CTCTTC 2 cut(s) 62, 414
BstC8I GCNNGC 1 cut(s) 613
BstDEI CTNAG 5 cut(s) 34, 150, 173, 179, 262
BstF5I GGATG 3 cut(s) 375, 448, 561
BstKTI GATC 1 cut(s) 561
BstMAI GTCTC 3 cut(s) 169, 177, 594
BstMBI GATC 1 cut(s) 558
BstMCI CGRYCG 1 cut(s) 579
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 276
BstSFI CTRYAG 1 cut(s) 484
BstV1I GCAGC 3 cut(s) 184, 539, 610
BstV2I GAAGAC 1 cut(s) 119
BstXI CCANNNNNNTGG 1 cut(s) 284
BtrI CACGTC 1 cut(s) 10
BtsCI GGATG 3 cut(s) 375, 448, 561
BtsI GCAGTG 1 cut(s) 535
BtsIMutI CAGTG 3 cut(s) 477, 526, 535
Cac8I GCNNGC 1 cut(s) 613
Csp6I GTAC 1 cut(s) 388
CviAII CATG 1 cut(s) 571
CviJI RGCY 9 cut(s) 137, 166, 197, 223, 266, 344, 530, 615, 637
CviKI_1 RGCY 9 cut(s) 137, 166, 197, 223, 266, 344, 530, 615, 637
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 5 cut(s) 34, 150, 173, 179, 262
DpnI GATC 1 cut(s) 560
DpnII GATC 1 cut(s) 558
DraI TTTAAA 1 cut(s) 205
DrdI GACNNNNNNGTC 1 cut(s) 326
DseDI GACNNNNNNGTC 1 cut(s) 326
Eam1104I CTCTTC 2 cut(s) 62, 414
EarI CTCTTC 2 cut(s) 62, 414
Eco31I GGTCTC 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 330
Eco88I CYCGRG 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 276
FaeI CATG 1 cut(s) 574
FaiI YATR 5 cut(s) 82, 84, 108, 194, 572
FaqI GGGAC 2 cut(s) 8, 290
FatI CATG 1 cut(s) 570
FblI GTMKAC 3 cut(s) 330, 483, 575
Fnu4HI GCNGC 3 cut(s) 198, 528, 624
FokI GGATG 3 cut(s) 362, 435, 568
Fsp4HI GCNGC 3 cut(s) 198, 528, 624
FspBI CTAG 3 cut(s) 123, 134, 700
GluI GCNGC 3 cut(s) 198, 528, 624
HapII CCGG 1 cut(s) 531
Hin1II CATG 1 cut(s) 574
HincII GTYRAC 1 cut(s) 576
HindII GTYRAC 1 cut(s) 576
HinfI GANTC 3 cut(s) 21, 398, 587
HpaII CCGG 1 cut(s) 531
HphI GGTGA 3 cut(s) 111, 415, 424
Hpy166II GTNNAC 5 cut(s) 98, 331, 484, 576, 631
Hpy188I TCNGA 3 cut(s) 151, 174, 349
Hpy188III TCNNGA 1 cut(s) 18
Hpy8I GTNNAC 5 cut(s) 98, 331, 484, 576, 631
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 1 cut(s) 388
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 2 cut(s) 142, 438
HpyF3I CTNAG 5 cut(s) 34, 150, 173, 179, 262
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 1 cut(s) 574
Kzo9I GATC 1 cut(s) 558
LpnPI CCDG 8 cut(s) 205, 263, 290, 325, 340, 472, 544, 597
Lsp1109I GCAGC 3 cut(s) 184, 539, 610
LweI GCATC 1 cut(s) 151
MaeI CTAG 3 cut(s) 123, 134, 700
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 3 cut(s) 5, 47, 412
MalI GATC 1 cut(s) 560
MboI GATC 1 cut(s) 558
MboII GAAGA 5 cut(s) 49, 119, 122, 431, 451
MluCI AATT 2 cut(s) 109, 439
MlyI GAGTC 3 cut(s) 30, 392, 596
MmeI TCCRAC 2 cut(s) 335, 489
MseI TTAA 4 cut(s) 87, 204, 384, 564
MspI CCGG 1 cut(s) 531
MspR9I CCNGG 1 cut(s) 278
MvaI CCWGG 1 cut(s) 278
NdeII GATC 1 cut(s) 558
NlaIII CATG 1 cut(s) 574
NlaIV GGNNCC 1 cut(s) 359
NmuCI GTSAC 2 cut(s) 5, 412
PkrI GCNGC 3 cut(s) 199, 529, 625
PleI GAGTC 3 cut(s) 29, 392, 595
PpsI GAGTC 3 cut(s) 29, 392, 595
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 359
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
SalI GTCGAC 1 cut(s) 574
SaqAI TTAA 4 cut(s) 87, 204, 384, 564
SatI GCNGC 3 cut(s) 198, 528, 624
Sau3AI GATC 1 cut(s) 558
SchI GAGTC 3 cut(s) 30, 392, 596
ScrFI CCNGG 1 cut(s) 278
SfaNI GCATC 1 cut(s) 151
SfcI CTRYAG 1 cut(s) 484
SmlI CTYRAG 1 cut(s) 291
SmoI CTYRAG 1 cut(s) 291
Sse9I AATT 2 cut(s) 109, 439
SsiI CCGC 1 cut(s) 626
SspMI CTAG 3 cut(s) 123, 134, 700
StyD4I CCNGG 1 cut(s) 276
TaaI ACNGT 1 cut(s) 481
TaiI ACGT 1 cut(s) 12
TaqI TCGA 1 cut(s) 575
TaqII GACCGA 1 cut(s) 593
TasI AATT 2 cut(s) 109, 439
TatI WGTACW 1 cut(s) 387
Tru1I TTAA 4 cut(s) 87, 204, 384, 564
Tru9I TTAA 4 cut(s) 87, 204, 384, 564
TscAI CASTG 3 cut(s) 484, 526, 542
TseFI GTSAC 2 cut(s) 5, 412
TseI GCWGC 3 cut(s) 197, 527, 623
Tsp45I GTSAC 2 cut(s) 5, 412
TspDTI ATGAA 2 cut(s) 223, 505
TspGWI ACGGA 3 cut(s) 368, 431, 582
TspRI CASTG 3 cut(s) 484, 526, 542
XmiI GTMKAC 3 cut(s) 330, 483, 575
XspI CTAG 3 cut(s) 123, 134, 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.