Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0015861

Physical Location & Seq

Genomic Coordinates Forward (+)

5107084 .. 5108132

Gene Structure 1049 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1073600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 807 bp

ATGGTAGCTATGATGGGTTGGGGTGGTGTTGCAGAACAAGGATGGAGGAAAGGTTCTTGGAGTCGTGAGGAGGATAAGCTGCTCAGTGAATATGTCAGCTTACATGGAGAGGGAAGATGGGGTTCTGTGGCTAGGTCTACAGGTTTGAAAAGAAGTGGGAAAAGTTGCAGACTCCGATGGGTAAATTATCTGAGGCCAGGACTAAAGAGAGGGCATATAACACCTCAGGAGGAAGACATCATCGTTGAATTGCATGCTTTATGGGGTAACAAATGGTCTACCATTGCAAGATACATGCCAGGGAGAACAGACAATGAGGTAAAGAACTTCTGGAGAACCCTTTTCAAAAGGAAAGAGAAATGCACTCAGAAGCAAGAGAAAAGAAAAGCTCAGATTCTTGAAGCAAAACAACAACAATTAGAGGAAGATCAAAACATGAAAACAAGAGCCACACCGCCACCACATCAAGATTTGGAAGCCTGCAGCCGAGGAATGCTAAACAATATGAATAATACTGATGTCCATGAAACACAAGCAAAAAGGCAAGAAAACATGGAGGAAGACAAATATTGCATGCCTAAATTGATGCACCAAGCAAGTGTTGAATTTTGGTCAGATTTATTAGGAGAAGGGCTTTATATGGGCAAGCTCTGGAACTTGGACGGCGTAAATGGTCGAATGGATCCGCTGCAACACACAAGCTTAGACCAGTGCAACAAAGTAGCCATGCAGATCAAATATCAAGGGATTACTGCAGCAACTTTATCTACTGGAGGGGAATCAAGCAATATATCAATGCACAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009719 GO:0009725 GO:0009751 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0014070 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0046677 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

269

Amino Acids

30.83

Weight (kDa)

9.01

Isoelectric Point (pI)

62.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	17 - 64	3.9e-16	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	20 - 78	1.8e-10	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	70 - 114	3.2e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	74 - 127	3.5e-08	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015861)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G30210
fragaria_vesca	FvH4_6g46440
malus_domestica	MD09G1073600.v1.1 MD17G1065100.v1.1
prunus_persica	Prupe.3G249600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0164981
rosa_laevigata	RLG00000021503
rosa_roxburghii	Rroxscaffold_2G00086140
rosa_rugosa	Rorug02G0511900 Rorug02G0512000
rosa_samantha	Rh2AG580100 Rh2BG591200 Rh2CG561700 Rh2DG601500
rosa_wichuraiana	Rw2G048150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	137, 278
AciI	CCGC	2 cut(s)	455, 686
AclWI	GGATC	2 cut(s)	677, 690
AcsI	RAATTY	1 cut(s)	605
AgsI	TTSAA	5 cut(s)	148, 248, 346, 401, 605
AjnI	CCWGG	2 cut(s)	196, 298
AloI	GAACNNNNNNTCC	2 cut(s)	37, 69
AluBI	AGCT	6 cut(s)	8, 79, 99, 389, 649, 702
AluI	AGCT	6 cut(s)	8, 79, 99, 389, 649, 702
AlwI	GGATC	2 cut(s)	677, 690
AoxI	GGCC	1 cut(s)	194
ApeKI	GCWGC	4 cut(s)	79, 483, 688, 755
ApoI	RAATTY	1 cut(s)	605
AxyI	CCTNAGG	1 cut(s)	225
BamHI	GGATCC	1 cut(s)	682
BbsI	GAAGAC	2 cut(s)	240, 567
BbvI	GCAGC	4 cut(s)	66, 495, 675, 767
BccI	CCATC	4 cut(s)	7, 36, 111, 171
BceAI	ACGGC	1 cut(s)	679
BciT130I	CCWGG	2 cut(s)	198, 300
BfaI	CTAG	1 cut(s)	132
BfmI	CTRYAG	3 cut(s)	138, 481, 753
BisI	GCNGC	4 cut(s)	80, 484, 689, 756
BlsI	GCNGC	4 cut(s)	81, 485, 690, 757
Bme1390I	CCNGG	2 cut(s)	198, 300
BmiI	GGNNCC	1 cut(s)	684
BmrFI	CCNGG	2 cut(s)	198, 300
BmsI	GCATC	1 cut(s)	576
BpiI	GAAGAC	2 cut(s)	240, 567
BpmI	CTGGAG	2 cut(s)	352, 792
BsaJI	CCNNGG	2 cut(s)	299, 487
BsaXI	ACNNNNNCTCC	2 cut(s)	37, 67
Bse1I	ACTGG	2 cut(s)	709, 775
Bse21I	CCTNAGG	1 cut(s)	225
Bse3DI	GCAATG	1 cut(s)	282
BseBI	CCWGG	2 cut(s)	198, 300
BseDI	CCNNGG	2 cut(s)	299, 487
BseGI	GGATG	1 cut(s)	47
BseMI	GCAATG	1 cut(s)	282
BseMII	CTCAG	5 cut(s)	97, 182, 239, 380, 404
BseNI	ACTGG	2 cut(s)	709, 775
BseRI	GAGGAG	1 cut(s)	83
BseXI	GCAGC	4 cut(s)	66, 495, 675, 767
BshFI	GGCC	1 cut(s)	196
BsmI	GAATGC	1 cut(s)	498
BsnI	GGCC	1 cut(s)	196
Bsp143I	GATC	3 cut(s)	427, 682, 732
BspACI	CCGC	2 cut(s)	455, 686
BspANI	GGCC	1 cut(s)	196
BspCNI	CTCAG	5 cut(s)	96, 183, 238, 379, 403
BspLI	GGNNCC	1 cut(s)	684
BspMAI	CTGCAG	2 cut(s)	485, 757
BspPI	GGATC	2 cut(s)	677, 690
BsrDI	GCAATG	1 cut(s)	282
BsrI	ACTGG	2 cut(s)	709, 775
BssECI	CCNNGG	2 cut(s)	299, 487
BssMI	GATC	3 cut(s)	427, 682, 732
Bst2UI	CCWGG	2 cut(s)	198, 300
BstC8I	GCNNGC	4 cut(s)	255, 481, 575, 647
BstDEI	CTNAG	6 cut(s)	83, 191, 225, 366, 390, 703
BstF5I	GGATG	1 cut(s)	47
BstKTI	GATC	3 cut(s)	430, 685, 735
BstMBI	GATC	3 cut(s)	427, 682, 732
BstNI	CCWGG	2 cut(s)	198, 300
BstNSI	RCATGY	3 cut(s)	257, 298, 577
BstSCI	CCNGG	2 cut(s)	196, 298
BstSFI	CTRYAG	3 cut(s)	138, 481, 753
BstV1I	GCAGC	4 cut(s)	66, 495, 675, 767
BstV2I	GAAGAC	2 cut(s)	240, 567
BstX2I	RGATCY	1 cut(s)	682
BstYI	RGATCY	1 cut(s)	682
Bsu36I	CCTNAGG	1 cut(s)	225
BsuRI	GGCC	1 cut(s)	196
BtsCI	GGATG	1 cut(s)	47
BtsIMutI	CAGTG	2 cut(s)	91, 716
Cac8I	GCNNGC	4 cut(s)	255, 481, 575, 647
CviAII	CATG	8 cut(s)	104, 254, 295, 436, 524, 553, 574, 727
DdeI	CTNAG	6 cut(s)	83, 191, 225, 366, 390, 703
DpnI	GATC	3 cut(s)	429, 684, 734
DpnII	GATC	3 cut(s)	427, 682, 732
Eco81I	CCTNAGG	1 cut(s)	225
EcoRII	CCWGG	2 cut(s)	196, 298
FaeI	CATG	8 cut(s)	107, 257, 298, 439, 527, 556, 577, 730
FatI	CATG	8 cut(s)	103, 253, 294, 435, 523, 552, 573, 726
FblI	GTMKAC	2 cut(s)	137, 278
Fnu4HI	GCNGC	4 cut(s)	80, 484, 689, 756
FokI	GGATG	1 cut(s)	54
Fsp4HI	GCNGC	4 cut(s)	80, 484, 689, 756
FspBI	CTAG	1 cut(s)	132
GluI	GCNGC	4 cut(s)	80, 484, 689, 756
GsuI	CTGGAG	2 cut(s)	352, 792
HaeIII	GGCC	1 cut(s)	196
Hin1II	CATG	8 cut(s)	107, 257, 298, 439, 527, 556, 577, 730
HindIII	AAGCTT	1 cut(s)	700
HinfI	GANTC	4 cut(s)	61, 171, 394, 779
Hpy166II	GTNNAC	2 cut(s)	138, 279
Hpy188I	TCNGA	5 cut(s)	176, 192, 369, 393, 616
Hpy188III	TCNNGA	6 cut(s)	65, 227, 331, 398, 467, 652
Hpy8I	GTNNAC	2 cut(s)	138, 279
HpyAV	CCTTC	1 cut(s)	623
HpyF3I	CTNAG	6 cut(s)	83, 191, 225, 366, 390, 703
Hsp92II	CATG	8 cut(s)	107, 257, 298, 439, 527, 556, 577, 730
Kzo9I	GATC	3 cut(s)	427, 682, 732
Lsp1109I	GCAGC	4 cut(s)	66, 495, 675, 767
LweI	GCATC	1 cut(s)	576
MaeI	CTAG	1 cut(s)	132
MaeIII	GTNAC	1 cut(s)	266
MalI	GATC	3 cut(s)	429, 684, 734
MboI	GATC	3 cut(s)	427, 682, 732
MboII	GAAGA	4 cut(s)	126, 245, 437, 572
MfeI	CAATTG	1 cut(s)	802
MflI	RGATCY	1 cut(s)	682
MluCI	AATT	6 cut(s)	184, 248, 416, 581, 605, 802
MlyI	GAGTC	2 cut(s)	70, 165
MspA1I	CMGCKG	1 cut(s)	688
MspR9I	CCNGG	2 cut(s)	198, 300
MunI	CAATTG	1 cut(s)	802
Mva1269I	GAATGC	1 cut(s)	498
MvaI	CCWGG	2 cut(s)	198, 300
NdeII	GATC	3 cut(s)	427, 682, 732
NlaIII	CATG	8 cut(s)	107, 257, 298, 439, 527, 556, 577, 730
NlaIV	GGNNCC	1 cut(s)	684
NmeAIII	GCCGAG	1 cut(s)	512
NspI	RCATGY	3 cut(s)	257, 298, 577
PaeI	GCATGC	2 cut(s)	257, 577
PctI	GAATGC	1 cut(s)	498
PfeI	GAWTC	2 cut(s)	394, 779
PkrI	GCNGC	4 cut(s)	81, 485, 690, 757
PleI	GAGTC	2 cut(s)	69, 165
PpsI	GAGTC	2 cut(s)	69, 165
Psp6I	CCWGG	2 cut(s)	196, 298
PspGI	CCWGG	2 cut(s)	196, 298
PspN4I	GGNNCC	1 cut(s)	684
PstI	CTGCAG	2 cut(s)	485, 757
PsuI	RGATCY	1 cut(s)	682
SatI	GCNGC	4 cut(s)	80, 484, 689, 756
Sau3AI	GATC	3 cut(s)	427, 682, 732
SchI	GAGTC	2 cut(s)	70, 165
ScrFI	CCNGG	2 cut(s)	198, 300
SfaNI	GCATC	1 cut(s)	576
SfcI	CTRYAG	3 cut(s)	138, 481, 753
SphI	GCATGC	2 cut(s)	257, 577
Sse9I	AATT	6 cut(s)	184, 248, 416, 581, 605, 802
SsiI	CCGC	2 cut(s)	455, 686
SspI	AATATT	1 cut(s)	569
SspMI	CTAG	1 cut(s)	132
StyD4I	CCNGG	2 cut(s)	196, 298
TaqI	TCGA	1 cut(s)	676
TasI	AATT	6 cut(s)	184, 248, 416, 581, 605, 802
TfiI	GAWTC	2 cut(s)	394, 779
TscAI	CASTG	2 cut(s)	91, 716
TseI	GCWGC	4 cut(s)	79, 483, 688, 755
TspDTI	ATGAA	3 cut(s)	452, 521, 540
TspRI	CASTG	2 cut(s)	91, 716
XapI	RAATTY	1 cut(s)	605
XceI	RCATGY	3 cut(s)	257, 298, 577
XmiI	GTMKAC	2 cut(s)	137, 278
XspI	CTAG	1 cut(s)	132

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.