MD09G1076500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015676
Physical Location & Seq
Reverse (-)
5393475 .. 5395276
1802 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1076500.v1.1.491

Sequence Viewer

Length: 747 bp
ATGATGCTACAGCTTACAAGCTTGGTAGTAGGGCTTCTTTTTGTTATGCATGCCCTAGGCCAGTTTAGCACTGCACATGCTCATCAAGGTAAAGGAGATATTTCAGTGACTGGGAAACATGCAGTATCGCCTGATCAACAGGAACCAAATGTTGATGGAATTTCAATAGCTGGGTCAAGGATGATAGGGCTTGGAGGAAGGAAAATGGTGGCACACAAAGTGTTAAGGAAATTAGAGCCAGCAAGACCAGCTGGGCAGCTGAATGAAGAGGCTAGTACCACTTCCAAGAATTCAGGTGCAAACCGTAGTAGTTCTGATGGAAATTGTGCCTTTGAAGGAAAGGGGGATCTAAATGTAAAATGTAAACTTGGAGATCGTGATAAAAGTAATACCAGCAAGGATAATTCTAAAGCTTCAAGCAATTCCCATGGAAAACCTCAAAACAAATTAAACCAACAGGATGATATGAAGACGCTGGAGCTAACAACATTGAAAAGTACTTCTGCCGGTAGTTTGGAAATTCCCAGAGATCTTAAACCAAATCACTCCCAAGATTTCAAGACAAAGCAGCCAACAACCAAAGCTCCAAAGACTATAAGTTATGACGAAAAAGATGATCATAAGTTGACTGATGAAGCAAGAAGGCTTCTTAAGGCGACAAGAGAGATAGTAAACCTGATGCATAAAGATTATCAACGAATGGGAAGCCGCAAGCCGCCGATACACAATTTTGAGCCACGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

27.13

Weight (kDa)

9.63

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 709, 716
AclWI GGATC 1 cut(s) 354
AcsI RAATTY 3 cut(s) 159, 289, 519
AdeI CACNNNGTG 1 cut(s) 220
AfaI GTAC 2 cut(s) 277, 499
AflII CTTAAG 1 cut(s) 650
AgsI TTSAA 5 cut(s) 165, 335, 417, 493, 559
AluBI AGCT 8 cut(s) 13, 21, 170, 251, 259, 413, 481, 584
AluI AGCT 8 cut(s) 13, 21, 170, 251, 259, 413, 481, 584
AlwI GGATC 1 cut(s) 354
AlwNI CAGNNNCTG 1 cut(s) 110
AoxI GGCC 1 cut(s) 58
ApeKI GCWGC 2 cut(s) 256, 568
ApoI RAATTY 3 cut(s) 159, 289, 519
AspA2I CCTAGG 1 cut(s) 55
AvrII CCTAGG 1 cut(s) 55
BarI GAAGNNNNNNTAC 2 cut(s) 18, 50
BbsI GAAGAC 1 cut(s) 476
BbvI GCAGC 2 cut(s) 268, 580
BccI CCATC 2 cut(s) 149, 311
BclI TGATCA 2 cut(s) 133, 616
BfaI CTAG 2 cut(s) 56, 273
BfmI CTRYAG 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 650
BglII AGATCT 1 cut(s) 529
BisI GCNGC 4 cut(s) 257, 569, 709, 716
BlnI CCTAGG 1 cut(s) 55
BlsI GCNGC 4 cut(s) 258, 570, 710, 717
BmcAI AGTACT 1 cut(s) 499
BmiI GGNNCC 1 cut(s) 144
BmrI ACTGGG 1 cut(s) 120
BmsI GCATC 1 cut(s) 669
BmuI ACTGGG 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 476
BpmI CTGGAG 1 cut(s) 497
BsaJI CCNNGG 3 cut(s) 55, 427, 737
BsaXI ACNNNNNCTCC 2 cut(s) 568, 598
Bse118I RCCGGY 1 cut(s) 506
Bse1I ACTGG 2 cut(s) 61, 115
BseDI CCNNGG 3 cut(s) 55, 427, 737
BseGI GGATG 2 cut(s) 186, 466
BseNI ACTGG 2 cut(s) 61, 115
BseXI GCAGC 2 cut(s) 268, 580
BseYI CCCAGC 2 cut(s) 170, 251
BsgI GTGCAG 1 cut(s) 57
BshFI GGCC 1 cut(s) 60
BsiSI CCGG 1 cut(s) 507
BsnI GGCC 1 cut(s) 60
Bsp143I GATC 5 cut(s) 133, 346, 373, 529, 616
Bsp19I CCATGG 1 cut(s) 427
BspACI CCGC 2 cut(s) 709, 716
BspANI GGCC 1 cut(s) 60
BspLI GGNNCC 1 cut(s) 144
BspPI GGATC 1 cut(s) 354
BspTI CTTAAG 1 cut(s) 650
BsrFI RCCGGY 1 cut(s) 506
BsrI ACTGG 2 cut(s) 61, 115
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 3 cut(s) 55, 427, 737
BssMI GATC 5 cut(s) 133, 346, 373, 529, 616
BssT1I CCWWGG 2 cut(s) 55, 427
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 1 cut(s) 261
BstAFI CTTAAG 1 cut(s) 650
BstC8I GCNNGC 3 cut(s) 51, 240, 713
BstDSI CCRYGG 2 cut(s) 427, 737
BstF5I GGATG 2 cut(s) 186, 466
BstKTI GATC 5 cut(s) 136, 349, 376, 532, 619
BstMBI GATC 5 cut(s) 133, 346, 373, 529, 616
BstMWI GCNNNNNNNGC 2 cut(s) 66, 248
BstNSI RCATGY 3 cut(s) 53, 80, 122
BstSFI CTRYAG 1 cut(s) 8
BstV1I GCAGC 2 cut(s) 268, 580
BstV2I GAAGAC 1 cut(s) 476
BstX2I RGATCY 2 cut(s) 346, 529
BstYI RGATCY 2 cut(s) 346, 529
BsuRI GGCC 1 cut(s) 60
BtgI CCRYGG 2 cut(s) 427, 737
BtsCI GGATG 2 cut(s) 186, 466
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 2 cut(s) 69, 111
Cac8I GCNNGC 3 cut(s) 51, 240, 713
CaiI CAGNNNCTG 1 cut(s) 110
Cfr10I RCCGGY 1 cut(s) 506
CseI GACGC 1 cut(s) 481
Csp6I GTAC 2 cut(s) 276, 498
CviAII CATG 4 cut(s) 50, 77, 119, 428
CviQI GTAC 2 cut(s) 276, 498
DpnI GATC 5 cut(s) 135, 348, 375, 531, 618
DpnII GATC 5 cut(s) 133, 346, 373, 529, 616
DraIII CACNNNGTG 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco130I CCWWGG 2 cut(s) 55, 427
EcoRI GAATTC 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 55, 427
EcoT22I ATGCAT 2 cut(s) 51, 684
ErhI CCWWGG 2 cut(s) 55, 427
FaeI CATG 4 cut(s) 53, 80, 122, 431
FatI CATG 4 cut(s) 49, 76, 118, 427
FbaI TGATCA 2 cut(s) 133, 616
Fnu4HI GCNGC 4 cut(s) 257, 569, 709, 716
FokI GGATG 2 cut(s) 193, 473
Fsp4HI GCNGC 4 cut(s) 257, 569, 709, 716
FspBI CTAG 2 cut(s) 56, 273
GluI GCNGC 4 cut(s) 257, 569, 709, 716
GsaI CCCAGC 2 cut(s) 174, 255
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 1 cut(s) 60
HapII CCGG 1 cut(s) 507
HgaI GACGC 1 cut(s) 481
Hin1II CATG 4 cut(s) 53, 80, 122, 431
HincII GTYRAC 1 cut(s) 627
HindII GTYRAC 1 cut(s) 627
HindIII AAGCTT 2 cut(s) 19, 411
HpaII CCGG 1 cut(s) 507
Hpy166II GTNNAC 3 cut(s) 365, 627, 673
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 2 cut(s) 377, 559
Hpy8I GTNNAC 3 cut(s) 365, 627, 673
HpyAV CCTTC 3 cut(s) 192, 329, 636
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4V TGCA 5 cut(s) 49, 74, 122, 299, 682
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 248
Hsp92II CATG 4 cut(s) 53, 80, 122, 431
Ksp22I TGATCA 2 cut(s) 133, 616
Kzo9I GATC 5 cut(s) 133, 346, 373, 529, 616
LmnI GCTCC 2 cut(s) 478, 589
Lsp1109I GCAGC 2 cut(s) 268, 580
LweI GCATC 1 cut(s) 669
MaeI CTAG 2 cut(s) 56, 273
MaeIII GTNAC 1 cut(s) 106
MalI GATC 5 cut(s) 135, 348, 375, 531, 618
MboI GATC 5 cut(s) 133, 346, 373, 529, 616
MboII GAAGA 2 cut(s) 278, 481
MflI RGATCY 2 cut(s) 346, 529
MluCI AATT 9 cut(s) 159, 230, 289, 322, 403, 421, 446, 519, 727
MnlI CCTC 3 cut(s) 188, 262, 447
Mph1103I ATGCAT 2 cut(s) 51, 684
MseI TTAA 4 cut(s) 224, 449, 534, 651
MspA1I CMGCKG 2 cut(s) 251, 259
MspCI CTTAAG 1 cut(s) 650
MspI CCGG 1 cut(s) 507
MwoI GCNNNNNNNGC 2 cut(s) 66, 248
NcoI CCATGG 1 cut(s) 427
NdeII GATC 5 cut(s) 133, 346, 373, 529, 616
NlaIII CATG 4 cut(s) 53, 80, 122, 431
NlaIV GGNNCC 1 cut(s) 144
NmuCI GTSAC 1 cut(s) 106
NsiI ATGCAT 2 cut(s) 51, 684
NspI RCATGY 3 cut(s) 53, 80, 122
PaeI GCATGC 1 cut(s) 53
PkrI GCNGC 4 cut(s) 258, 570, 710, 717
PspFI CCCAGC 2 cut(s) 170, 251
PspN4I GGNNCC 1 cut(s) 144
PstNI CAGNNNCTG 1 cut(s) 110
PsuI RGATCY 2 cut(s) 346, 529
PvuII CAGCTG 2 cut(s) 251, 259
RsaI GTAC 2 cut(s) 277, 499
RsaNI GTAC 2 cut(s) 276, 498
SaqAI TTAA 4 cut(s) 224, 449, 534, 651
SatI GCNGC 4 cut(s) 257, 569, 709, 716
Sau3AI GATC 5 cut(s) 133, 346, 373, 529, 616
ScaI AGTACT 1 cut(s) 499
SfaNI GCATC 1 cut(s) 669
SfcI CTRYAG 1 cut(s) 8
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
SphI GCATGC 1 cut(s) 53
Sse9I AATT 9 cut(s) 159, 230, 289, 322, 403, 421, 446, 519, 727
SsiI CCGC 2 cut(s) 709, 716
SspMI CTAG 2 cut(s) 56, 273
StyI CCWWGG 2 cut(s) 55, 427
TaaI ACNGT 1 cut(s) 305
TasI AATT 9 cut(s) 159, 230, 289, 322, 403, 421, 446, 519, 727
TatI WGTACW 1 cut(s) 497
TauI GCSGC 2 cut(s) 711, 718
Tru1I TTAA 4 cut(s) 224, 449, 534, 651
Tru9I TTAA 4 cut(s) 224, 449, 534, 651
TscAI CASTG 2 cut(s) 76, 111
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 2 cut(s) 256, 568
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 3 cut(s) 279, 482, 648
TspRI CASTG 2 cut(s) 76, 111
Vha464I CTTAAG 1 cut(s) 650
XapI RAATTY 3 cut(s) 159, 289, 519
XceI RCATGY 3 cut(s) 53, 80, 122
XmaJI CCTAGG 1 cut(s) 55
XspI CTAG 2 cut(s) 56, 273
ZrmI AGTACT 1 cut(s) 499
Zsp2I ATGCAT 2 cut(s) 51, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.