MD17G1067000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015676
Physical Location & Seq
Reverse (-)
5403233 .. 5405019
1787 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1067000.v1.1.491

Sequence Viewer

Length: 810 bp
ATGATGCTACAGCTTACAAGCTTGGTAGTAGGGCTTCTTTTTGGTATGCTTGCCCTAGGTCAGTTTAGCACTGCACATGCTCATCAAGGTAAAGAAGATATTTCAATGACTGGGAAACGTGCAGTATTGCCTGATCAAAAGGAACCAAATGTTGATGGAATTTCAATAGCTGGGTCAAGGATGATAGGGCTTGGAGGAAGGAAAATGGTGGCACACAGAGTGTTAAGGAAATCAGAGCCAGTAAGACCAGATGGGCAGCAAAATGAAGAGGCTAGTACCACTTCCAAGAATCCAGGTGCAAACCGTAGTACTTCTGATGGAAATTGTAACTTTGAAGGAAGGGAAGATCTAAATGTAAAATGTAAACTTGGAGATCGTGATAAAAGTAATACTAGTACTGCTAGTTCTAAGAAGTACTCCAAAGTCTCTCATCATGATTTTGTAGCTTTTGGTAAGGATAATCCTAAAGCTTCAAGCAATTTCAGTGGAAAACTTCAAAACCAAATGAACCAACAGGATAATGTGAAGATACTGGAGCTAACAACATTGAAAAGTACTTCTGCCGGTAGTTTGGAAATTCCCAGAGATCTTATACCAAATCACTCCGAAGATTTCAAGACAAAGCACCCAACAACCGAAGCTCAAATGACTAAAAGTTATGATGAAAAAGACGATCATAAGTTGACAGATAATGAAGCAAGAAGCCTTCTTAAGGCGACAAGAGAGATAGCAAACCTGATGCGTAAAGATTACCACCATCAAATGGGCCGCCGCAAGCCGCCAGTACACAATCTTCAGCCACGGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

29.83

Weight (kDa)

9.48

Isoelectric Point (pI)

30.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 763
AciI CCGC 3 cut(s) 769, 772, 779
AcsI RAATTY 2 cut(s) 159, 576
AcuI CTGAAG 1 cut(s) 779
AdeI CACNNNGTG 1 cut(s) 220
AfaI GTAC 6 cut(s) 277, 310, 397, 416, 556, 786
AfiI CCNNNNNNNGG 2 cut(s) 712, 763
AflII CTTAAG 1 cut(s) 710
AgsI TTSAA 7 cut(s) 105, 165, 335, 474, 497, 550, 616
AhlI ACTAGT 1 cut(s) 392
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 7 cut(s) 13, 21, 170, 446, 470, 538, 641
AluI AGCT 7 cut(s) 13, 21, 170, 446, 470, 538, 641
Alw26I GTCTC 1 cut(s) 430
AoxI GGCC 2 cut(s) 766, 803
ApeKI GCWGC 1 cut(s) 256
ApoI RAATTY 2 cut(s) 159, 576
AspA2I CCTAGG 1 cut(s) 55
AspS9I GGNCC 1 cut(s) 766
AvrII CCTAGG 1 cut(s) 55
BarI GAAGNNNNNNTAC 4 cut(s) 18, 50, 777, 809
BbvI GCAGC 1 cut(s) 268
BccI CCATC 4 cut(s) 149, 245, 311, 765
BciT130I CCWGG 1 cut(s) 294
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 430
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 4 cut(s) 56, 273, 393, 402
BfmI CTRYAG 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 710
BglII AGATCT 2 cut(s) 346, 586
BisI GCNGC 4 cut(s) 257, 769, 772, 779
BlnI CCTAGG 1 cut(s) 55
BlsI GCNGC 4 cut(s) 258, 770, 773, 780
BmcAI AGTACT 4 cut(s) 310, 397, 416, 556
Bme1390I CCNGG 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 766
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 1 cut(s) 294
BmrI ACTGGG 1 cut(s) 120
BmsI GCATC 1 cut(s) 729
BmuI ACTGGG 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 554
BsaJI CCNNGG 2 cut(s) 55, 800
Bsc4I CCNNNNNNNGG 2 cut(s) 712, 763
Bse118I RCCGGY 1 cut(s) 563
Bse1I ACTGG 4 cut(s) 115, 239, 537, 782
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 55, 800
BseGI GGATG 1 cut(s) 186
BseLI CCNNNNNNNGG 2 cut(s) 712, 763
BseNI ACTGG 4 cut(s) 115, 239, 537, 782
BseXI GCAGC 1 cut(s) 268
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 2 cut(s) 57, 141
BshFI GGCC 2 cut(s) 768, 805
BsiSI CCGG 1 cut(s) 564
BslI CCNNNNNNNGG 2 cut(s) 712, 763
BsmAI GTCTC 1 cut(s) 430
BsnI GGCC 2 cut(s) 768, 805
Bsp143I GATC 5 cut(s) 133, 346, 373, 586, 673
BspACI CCGC 3 cut(s) 769, 772, 779
BspANI GGCC 2 cut(s) 768, 805
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 1 cut(s) 144
BspTI CTTAAG 1 cut(s) 710
BsrFI RCCGGY 1 cut(s) 563
BsrI ACTGG 4 cut(s) 115, 239, 537, 782
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 2 cut(s) 55, 800
BssMI GATC 5 cut(s) 133, 346, 373, 586, 673
BssT1I CCWWGG 1 cut(s) 55
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 1 cut(s) 261
BstAFI CTTAAG 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 51, 776
BstDEI CTNAG 1 cut(s) 408
BstDSI CCRYGG 1 cut(s) 800
BstENI CCTNNNNNAGG 1 cut(s) 710
BstF5I GGATG 1 cut(s) 186
BstKTI GATC 5 cut(s) 136, 349, 376, 589, 676
BstMAI GTCTC 1 cut(s) 430
BstMBI GATC 5 cut(s) 133, 346, 373, 586, 673
BstNI CCWGG 1 cut(s) 294
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 1 cut(s) 292
BstSFI CTRYAG 1 cut(s) 8
BstV1I GCAGC 1 cut(s) 268
BstX2I RGATCY 2 cut(s) 346, 586
BstYI RGATCY 2 cut(s) 346, 586
BsuRI GGCC 2 cut(s) 768, 805
BtgI CCRYGG 1 cut(s) 800
BtsCI GGATG 1 cut(s) 186
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 2 cut(s) 69, 490
Cac8I GCNNGC 2 cut(s) 51, 776
CciI TCATGA 1 cut(s) 433
Cfr10I RCCGGY 1 cut(s) 563
Cfr13I GGNCC 1 cut(s) 766
Csp6I GTAC 6 cut(s) 276, 309, 396, 415, 555, 785
CspCI CAANNNNNGTGG 2 cut(s) 466, 501
CviAII CATG 2 cut(s) 77, 434
CviQI GTAC 6 cut(s) 276, 309, 396, 415, 555, 785
DdeI CTNAG 1 cut(s) 408
DpnI GATC 5 cut(s) 135, 348, 375, 588, 675
DpnII GATC 5 cut(s) 133, 346, 373, 586, 673
DraIII CACNNNGTG 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 779
EcoNI CCTNNNNNAGG 1 cut(s) 710
EcoRII CCWGG 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 55
FaeI CATG 2 cut(s) 80, 437
FaiI YATR 6 cut(s) 47, 78, 435, 593, 660, 678
FatI CATG 2 cut(s) 76, 433
FbaI TGATCA 1 cut(s) 133
Fnu4HI GCNGC 4 cut(s) 257, 769, 772, 779
FokI GGATG 1 cut(s) 193
Fsp4HI GCNGC 4 cut(s) 257, 769, 772, 779
FspBI CTAG 4 cut(s) 56, 273, 393, 402
GluI GCNGC 4 cut(s) 257, 769, 772, 779
GsaI CCCAGC 1 cut(s) 174
GsuI CTGGAG 1 cut(s) 554
HaeIII GGCC 2 cut(s) 768, 805
HapII CCGG 1 cut(s) 564
Hin1II CATG 2 cut(s) 80, 437
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HindIII AAGCTT 2 cut(s) 19, 468
HinfI GANTC 1 cut(s) 289
HpaII CCGG 1 cut(s) 564
Hpy166II GTNNAC 3 cut(s) 365, 684, 787
Hpy188I TCNGA 3 cut(s) 235, 316, 607
Hpy188III TCNNGA 3 cut(s) 377, 434, 616
Hpy8I GTNNAC 3 cut(s) 365, 684, 787
HpyAV CCTTC 4 cut(s) 192, 329, 333, 716
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 3 cut(s) 74, 122, 299
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 2 cut(s) 80, 437
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 5 cut(s) 133, 346, 373, 586, 673
LmnI GCTCC 1 cut(s) 535
Lsp1109I GCAGC 1 cut(s) 268
LweI GCATC 1 cut(s) 729
MaeI CTAG 4 cut(s) 56, 273, 393, 402
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 1 cut(s) 326
MalI GATC 5 cut(s) 135, 348, 375, 588, 675
MboI GATC 5 cut(s) 133, 346, 373, 586, 673
MboII GAAGA 6 cut(s) 107, 278, 356, 538, 620, 785
MflI RGATCY 2 cut(s) 346, 586
MluCI AATT 4 cut(s) 159, 322, 478, 576
MnlI CCTC 2 cut(s) 188, 262
MseI TTAA 2 cut(s) 224, 711
MspCI CTTAAG 1 cut(s) 710
MspI CCGG 1 cut(s) 564
MspR9I CCNGG 1 cut(s) 294
MvaI CCWGG 1 cut(s) 294
NdeII GATC 5 cut(s) 133, 346, 373, 586, 673
NlaIII CATG 2 cut(s) 80, 437
NlaIV GGNNCC 1 cut(s) 144
NspI RCATGY 1 cut(s) 80
PagI TCATGA 1 cut(s) 433
PfeI GAWTC 1 cut(s) 289
PflMI CCANNNNNTGG 1 cut(s) 763
PkrI GCNGC 4 cut(s) 258, 770, 773, 780
Psp6I CCWGG 1 cut(s) 292
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 1 cut(s) 766
PsrI GAACNNNNNNTAC 2 cut(s) 388, 420
PsuI RGATCY 2 cut(s) 346, 586
RsaI GTAC 6 cut(s) 277, 310, 397, 416, 556, 786
RsaNI GTAC 6 cut(s) 276, 309, 396, 415, 555, 785
SaqAI TTAA 2 cut(s) 224, 711
SatI GCNGC 4 cut(s) 257, 769, 772, 779
Sau3AI GATC 5 cut(s) 133, 346, 373, 586, 673
Sau96I GGNCC 1 cut(s) 766
ScaI AGTACT 4 cut(s) 310, 397, 416, 556
ScrFI CCNGG 1 cut(s) 294
SfaNI GCATC 1 cut(s) 729
SfcI CTRYAG 1 cut(s) 8
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
SpeI ACTAGT 1 cut(s) 392
Sse9I AATT 4 cut(s) 159, 322, 478, 576
SsiI CCGC 3 cut(s) 769, 772, 779
SspMI CTAG 4 cut(s) 56, 273, 393, 402
StyD4I CCNGG 1 cut(s) 292
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 1 cut(s) 121
TasI AATT 4 cut(s) 159, 322, 478, 576
TatI WGTACW 5 cut(s) 308, 395, 414, 554, 784
TauI GCSGC 3 cut(s) 771, 774, 781
TfiI GAWTC 1 cut(s) 289
Tru1I TTAA 2 cut(s) 224, 711
Tru9I TTAA 2 cut(s) 224, 711
TscAI CASTG 2 cut(s) 76, 490
TseI GCWGC 1 cut(s) 256
TspDTI ATGAA 4 cut(s) 279, 521, 678, 708
TspRI CASTG 2 cut(s) 76, 490
Van91I CCANNNNNTGG 1 cut(s) 763
Vha464I CTTAAG 1 cut(s) 710
XagI CCTNNNNNAGG 1 cut(s) 710
XapI RAATTY 2 cut(s) 159, 576
XceI RCATGY 1 cut(s) 80
XmaJI CCTAGG 1 cut(s) 55
XspI CTAG 4 cut(s) 56, 273, 393, 402
ZrmI AGTACT 4 cut(s) 310, 397, 416, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.