MD09G1103800.v1.1

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006751
Physical Location & Seq
Reverse (-)
7585347 .. 7588218
2872 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1103800.v1.1.491

Sequence Viewer

Length: 1533 bp
ATGCTCAAAAACTATGTGCCGATCTTCGTGATGCTCCCTTTGGGAGTTGTTACGGTTGACAATGTTTTGGAAGACGGGGACAAACTCGTTAAGGACCTGAAGGAGTTACGGGCAGCGGGTGTTGATGGGGTGATGATAGATGTGTGGTGGGGGATCATAGAATCGAAAGGGCCTAAGCAGTATGACTGGAGTGCTTATAGGAGCGTGTTTCAAGCAGTTGAAGATTGTGGGCTGAAATTACAAGCTATAATGTCATTCCACCAATGTGGTGGGAATGTAGGAGATGTTGTAACCATCCCCATTCCCAAATGGGTACTTGAGATCGGAGAATCAGACCCCGATATATTTTATACCAATCTCAAAGGTAACAGGAACCCGGAATACCTCACTCTAGGTGTGGATAACCTGCATCTCTTTGACGGACGAACCGCTGTGCAGATATACGGTGATTACATGAAAAGTTTCAGAGAGAACATGTCGGATTTTCTAGAAACCGGACTTGTTATAGACATTGAAGTAGGGCTTGGACCTGCAGGAGAGCTCAGGTATCCCTCTTATCCGGAAAGTCAAGGATGGGCTTTTCCAGGGATCGGAGAGTTTCAGTGCTTTGACAAGTATCTCCAAGCAGAGTTCAAAGAGGCTGCAACAGCTGCAGGTCATCCTGAGTGGGCTTTGCCAGATAACGCCGGAGAATATAATGACACTCCTGAATCCTCGGAGTTCTTCAGATCAAATGGGACATATGTTACTGAGAAAGGGAAGTTCTTCTTGACTTGGTACTCTAACAAATTAATGATCCATGGTGATCAGATCCTGGATGAAGCCAACAAAGTTTTTGTGGGCTGTAAATTAAAGCTAGCAGCCAAAGTATCTGGAATCCACTGGTGGTATGAAGTTGATAATCACGCCGCGGAGCTTACCGCAGGATACTACAACTTGAAAGATAGAGATGGGTACCGACCTATAGCCAGGATGCTGTCAAGGCATCATGCCATTTTGAATTTCACATGTTTAGAGATGAGGAACTCTGAACAAAGTGCTGATGCCAAAAGTGCACCTGAGGAACTTGTTCAGCAGGTTTTGAGTGGAGGTTGGAGAGAAAACATAGAAGTTGCCGGAGAAAATGCACTTTCTAGATACGACAGTGTGGCTTATGACCAAATCCTTCTAAATGCTAGACCAAATGGCATCAATAAAGACGGCCAACCAAAATTAAGGATGTATGGGGTGACATACCTTCGTTTATCTGATGAACTACTGCAAAAGACGAATCTTGATATATTCAAGATATTTGTAAAGAAGATGCATGCTGATCAGGATTACTGTCCAGACCCAGAGAAGTACAATCACCACCTAGGTCCTTTGGAGAGGTCGAAACCAGAGATTTCGATTGAAGATCTTCTGGAAGCAACCAAAGCAGAGGAGCCATTCCCATGGGATAAAGAAACAGATATGAGTGTCAGTGGTGGAGCTGGTGGACTTGTTGGCCTACTCTCTAATTTGATAAGCAAAATCTTAGCTCTATTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

511

Amino Acids

57.37

Weight (kDa)

4.93

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 9 - 419 6.9e-134 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 414, 538, 644, 1066
Acc65I GGTACC 1 cut(s) 954
AccB1I GGYRCC 1 cut(s) 954
AccII CGCG 1 cut(s) 911
AccIII TCCGGA 1 cut(s) 559
AciI CCGC 5 cut(s) 116, 429, 909, 911, 921
AclWI GGATC 4 cut(s) 161, 596, 790, 805
AcoI YGGCCR 1 cut(s) 1201
AcsI RAATTY 1 cut(s) 1000
AcuI CTGAAG 2 cut(s) 119, 709
AfaI GTAC 4 cut(s) 315, 779, 956, 1343
AfiI CCNNNNNNNGG 1 cut(s) 590
AflIII ACRYGT 2 cut(s) 474, 1007
AgsI TTSAA 8 cut(s) 212, 221, 515, 634, 940, 1000, 1285, 1394
AjnI CCWGG 3 cut(s) 583, 813, 968
AjuI GAANNNNNNNTTGG 2 cut(s) 507, 539
AleI CACNNNNGTG 1 cut(s) 264
AluBI AGCT 7 cut(s) 245, 541, 650, 856, 916, 1472, 1520
AluI AGCT 7 cut(s) 245, 541, 650, 856, 916, 1472, 1520
Alw21I GWGCWC 2 cut(s) 543, 1057
Alw44I GTGCAC 1 cut(s) 1053
AlwI GGATC 4 cut(s) 161, 596, 790, 805
AlwNI CAGNNNCTG 1 cut(s) 814
Aor13HI TCCGGA 1 cut(s) 559
AoxI GGCC 3 cut(s) 170, 1201, 1486
ApaLI GTGCAC 1 cut(s) 1053
ApeKI GCWGC 4 cut(s) 113, 641, 650, 860
ApoI RAATTY 1 cut(s) 1000
AseI ATTAAT 1 cut(s) 791
Asp700I GAANNNNTTC 4 cut(s) 461, 764, 1068, 1398
Asp718I GGTACC 1 cut(s) 954
AspA2I CCTAGG 1 cut(s) 1354
AspS9I GGNCC 4 cut(s) 94, 170, 527, 1358
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 5 cut(s) 142, 458, 815, 1240, 1340
AsuNHI GCTAGC 1 cut(s) 856
AvaII GGWCC 3 cut(s) 94, 527, 1358
AvrII CCTAGG 1 cut(s) 1354
AxyI CCTNAGG 1 cut(s) 1059
BaeGI GKGCMC 1 cut(s) 1057
BaeI ACNNNNGTAYC 2 cut(s) 1129, 1162
BanI GGYRCC 1 cut(s) 954
BanII GRGCYC 1 cut(s) 543
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 2 cut(s) 543, 1057
BbvI GCAGC 4 cut(s) 125, 628, 637, 872
BccI CCATC 4 cut(s) 119, 302, 567, 944
BceAI ACGGC 1 cut(s) 1216
BciT130I CCWGG 3 cut(s) 585, 815, 970
BciVI GTATCC 2 cut(s) 558, 920
BclI TGATCA 2 cut(s) 805, 1312
BcnI CCSGG 1 cut(s) 377
BfaI CTAG 6 cut(s) 392, 488, 857, 1134, 1176, 1355
BfmI CTRYAG 3 cut(s) 531, 651, 963
BfuAI ACCTGC 4 cut(s) 414, 538, 644, 1066
BfuI GTATCC 2 cut(s) 558, 920
BglII AGATCT 1 cut(s) 1396
BisI GCNGC 5 cut(s) 114, 642, 651, 861, 909
BlnI CCTAGG 1 cut(s) 1354
BlsI GCNGC 5 cut(s) 115, 643, 652, 862, 910
Bme1390I CCNGG 4 cut(s) 377, 585, 815, 970
Bme18I GGWCC 3 cut(s) 94, 527, 1358
BmgT120I GGNCC 4 cut(s) 94, 170, 527, 1358
BmiI GGNNCC 3 cut(s) 374, 956, 1425
BmrFI CCNGG 4 cut(s) 377, 585, 815, 970
BmsI GCATC 7 cut(s) 21, 418, 963, 994, 1033, 1197, 1293
BmtI GCTAGC 1 cut(s) 860
BpiI GAAGAC 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 208
Bpu10I CCTNAGC 2 cut(s) 174, 542
BpuEI CTTGAG 1 cut(s) 338
BpuMI CCSGG 1 cut(s) 377
BsaJI CCNNGG 6 cut(s) 584, 714, 799, 909, 1354, 1433
BsaWI WCCGGW 2 cut(s) 494, 559
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 1 cut(s) 590
Bse1I ACTGG 2 cut(s) 191, 887
Bse21I CCTNAGG 1 cut(s) 1059
BseAI TCCGGA 1 cut(s) 559
BseBI CCWGG 3 cut(s) 585, 815, 970
BseDI CCNNGG 6 cut(s) 584, 714, 799, 909, 1354, 1433
BseGI GGATG 6 cut(s) 294, 578, 658, 823, 978, 1224
BseLI CCNNNNNNNGG 1 cut(s) 590
BseMII CTCAG 4 cut(s) 556, 654, 741, 1050
BseNI ACTGG 2 cut(s) 191, 887
BseRI GAGGAG 1 cut(s) 1436
BseSI GKGCMC 1 cut(s) 1057
BseXI GCAGC 4 cut(s) 125, 628, 637, 872
BsgI GTGCAG 1 cut(s) 455
Bsh1236I CGCG 1 cut(s) 911
BshFI GGCC 3 cut(s) 172, 1203, 1488
BshNI GGYRCC 1 cut(s) 954
BsiHKAI GWGCWC 2 cut(s) 543, 1057
BsiSI CCGG 5 cut(s) 377, 495, 560, 687, 1116
BslFI GGGAC 2 cut(s) 92, 751
BslI CCNNNNNNNGG 1 cut(s) 590
BsmFI GGGAC 2 cut(s) 92, 751
BsnI GGCC 3 cut(s) 172, 1203, 1488
Bsp1286I GDGCHC 2 cut(s) 543, 1057
Bsp13I TCCGGA 1 cut(s) 559
Bsp19I CCATGG 2 cut(s) 799, 1433
BspACI CCGC 5 cut(s) 116, 429, 909, 911, 921
BspANI GGCC 3 cut(s) 172, 1203, 1488
BspCNI CTCAG 4 cut(s) 555, 655, 742, 1051
BspEI TCCGGA 1 cut(s) 559
BspFNI CGCG 1 cut(s) 911
BspLI GGNNCC 3 cut(s) 374, 956, 1425
BspMAI CTGCAG 2 cut(s) 535, 655
BspMI ACCTGC 4 cut(s) 414, 538, 644, 1066
BspOI GCTAGC 1 cut(s) 860
BspPI GGATC 4 cut(s) 161, 596, 790, 805
BspT107I GGYRCC 1 cut(s) 954
BsrI ACTGG 2 cut(s) 191, 887
BssECI CCNNGG 6 cut(s) 584, 714, 799, 909, 1354, 1433
BssT1I CCWWGG 3 cut(s) 799, 1354, 1433
Bst2UI CCWGG 3 cut(s) 585, 815, 970
Bst4CI ACNGT 4 cut(s) 55, 446, 1145, 1325
BstAPI GCANNNNNTGC 1 cut(s) 650
BstC8I GCNNGC 2 cut(s) 858, 1308
BstDEI CTNAG 6 cut(s) 174, 542, 663, 750, 1059, 1516
BstDSI CCRYGG 3 cut(s) 799, 909, 1433
BstF5I GGATG 6 cut(s) 294, 578, 658, 823, 978, 1224
BstFNI CGCG 1 cut(s) 911
BstMWI GCNNNNNNNGC 5 cut(s) 647, 650, 982, 1052, 1415
BstNI CCWGG 3 cut(s) 585, 815, 970
BstNSI RCATGY 3 cut(s) 478, 1011, 1310
BstSCI CCNGG 4 cut(s) 375, 583, 813, 968
BstSFI CTRYAG 3 cut(s) 531, 651, 963
BstSLI GKGCMC 1 cut(s) 1057
BstUI CGCG 1 cut(s) 911
BstV1I GCAGC 4 cut(s) 125, 628, 637, 872
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 2 cut(s) 810, 1396
BstXI CCANNNNNNTGG 3 cut(s) 266, 269, 1434
BstYI RGATCY 2 cut(s) 810, 1396
Bsu36I CCTNAGG 1 cut(s) 1059
BsuI GTATCC 2 cut(s) 558, 920
BsuRI GGCC 3 cut(s) 172, 1203, 1488
BtgI CCRYGG 3 cut(s) 799, 909, 1433
BtsCI GGATG 6 cut(s) 294, 578, 658, 823, 978, 1224
BtsIMutI CAGTG 4 cut(s) 608, 880, 1150, 1468
BveI ACCTGC 4 cut(s) 414, 538, 644, 1066
Cac8I GCNNGC 2 cut(s) 858, 1308
CaiI CAGNNNCTG 1 cut(s) 814
Cfr13I GGNCC 4 cut(s) 94, 170, 527, 1358
Cfr42I CCGCGG 1 cut(s) 912
Csp6I GTAC 4 cut(s) 314, 778, 955, 1342
CviAII CATG 7 cut(s) 454, 475, 800, 989, 1008, 1307, 1434
CviQI GTAC 4 cut(s) 314, 778, 955, 1342
DdeI CTNAG 6 cut(s) 174, 542, 663, 750, 1059, 1516
EaeI YGGCCR 1 cut(s) 1201
Ecl136II GAGCTC 1 cut(s) 541
Eco130I CCWWGG 3 cut(s) 799, 1354, 1433
Eco24I GRGCYC 1 cut(s) 543
Eco47I GGWCC 3 cut(s) 94, 527, 1358
Eco53kI GAGCTC 1 cut(s) 541
Eco57I CTGAAG 2 cut(s) 119, 709
Eco81I CCTNAGG 1 cut(s) 1059
EcoICRI GAGCTC 1 cut(s) 541
EcoO109I RGGNCCY 3 cut(s) 94, 170, 1358
EcoRII CCWGG 3 cut(s) 583, 813, 968
EcoT14I CCWWGG 3 cut(s) 799, 1354, 1433
EcoT22I ATGCAT 1 cut(s) 1308
EcoT38I GRGCYC 1 cut(s) 543
ErhI CCWWGG 3 cut(s) 799, 1354, 1433
FaeI CATG 7 cut(s) 457, 478, 803, 992, 1011, 1310, 1437
FalI AAGNNNNNCTT 4 cut(s) 507, 539, 752, 784
FaqI GGGAC 2 cut(s) 92, 751
FatI CATG 7 cut(s) 453, 474, 799, 988, 1007, 1306, 1433
FauI CCCGC 1 cut(s) 109
FauNDI CATATG 1 cut(s) 742
FbaI TGATCA 2 cut(s) 805, 1312
Fnu4HI GCNGC 5 cut(s) 114, 642, 651, 861, 909
FokI GGATG 6 cut(s) 281, 585, 645, 830, 985, 1231
FriOI GRGCYC 1 cut(s) 543
Fsp4HI GCNGC 5 cut(s) 114, 642, 651, 861, 909
FspBI CTAG 6 cut(s) 392, 488, 857, 1134, 1176, 1355
GluI GCNGC 5 cut(s) 114, 642, 651, 861, 909
GsuI CTGGAG 1 cut(s) 208
HaeIII GGCC 3 cut(s) 172, 1203, 1488
HapII CCGG 5 cut(s) 377, 495, 560, 687, 1116
Hin1II CATG 7 cut(s) 457, 478, 803, 992, 1011, 1310, 1437
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 5 cut(s) 161, 329, 710, 876, 1270
HpaII CCGG 5 cut(s) 377, 495, 560, 687, 1116
HphI GGTGA 5 cut(s) 142, 458, 815, 1240, 1340
Hpy166II GTNNAC 3 cut(s) 58, 1055, 1478
Hpy8I GTNNAC 3 cut(s) 58, 1055, 1478
HpyAV CCTTC 3 cut(s) 94, 1175, 1247
HpyCH4III ACNGT 4 cut(s) 55, 446, 1145, 1325
HpyCH4V TGCA 9 cut(s) 409, 436, 533, 644, 653, 1055, 1127, 1261, 1306
HpyF10VI GCNNNNNNNGC 5 cut(s) 647, 650, 982, 1052, 1415
HpyF3I CTNAG 6 cut(s) 174, 542, 663, 750, 1059, 1516
Hsp92II CATG 7 cut(s) 457, 478, 803, 992, 1011, 1310, 1437
Kpn2I TCCGGA 1 cut(s) 559
KpnI GGTACC 1 cut(s) 958
Ksp22I TGATCA 2 cut(s) 805, 1312
KspI CCGCGG 1 cut(s) 912
LmnI GCTCC 5 cut(s) 39, 201, 913, 1423, 1469
Lsp1109I GCAGC 4 cut(s) 125, 628, 637, 872
LweI GCATC 7 cut(s) 21, 418, 963, 994, 1033, 1197, 1293
MaeI CTAG 6 cut(s) 392, 488, 857, 1134, 1176, 1355
MaeIII GTNAC 6 cut(s) 49, 105, 289, 365, 745, 1228
MboII GAAGA 8 cut(s) 16, 83, 233, 715, 757, 1312, 1391, 1406
MflI RGATCY 2 cut(s) 810, 1396
MhlI GDGCHC 2 cut(s) 543, 1057
MluCI AATT 6 cut(s) 236, 788, 848, 1000, 1211, 1498
MmeI TCCRAC 2 cut(s) 459, 1073
MnlI CCTC 9 cut(s) 395, 562, 631, 724, 1014, 1054, 1082, 1362, 1414
Mph1103I ATGCAT 1 cut(s) 1308
MroI TCCGGA 1 cut(s) 559
MroXI GAANNNNTTC 4 cut(s) 461, 764, 1068, 1398
MseI TTAA 5 cut(s) 90, 791, 851, 1214, 1527
MslI CAYNNNNRTG 2 cut(s) 264, 1432
MspA1I CMGCKG 4 cut(s) 116, 431, 650, 911
MspI CCGG 5 cut(s) 377, 495, 560, 687, 1116
MspR9I CCNGG 4 cut(s) 377, 585, 815, 970
MvaI CCWGG 3 cut(s) 585, 815, 970
MvnI CGCG 1 cut(s) 911
MwoI GCNNNNNNNGC 5 cut(s) 647, 650, 982, 1052, 1415
NciI CCSGG 1 cut(s) 377
NcoI CCATGG 2 cut(s) 799, 1433
NdeI CATATG 1 cut(s) 742
NheI GCTAGC 1 cut(s) 856
NlaIII CATG 7 cut(s) 457, 478, 803, 992, 1011, 1310, 1437
NlaIV GGNNCC 3 cut(s) 374, 956, 1425
NmuCI GTSAC 1 cut(s) 1228
NsiI ATGCAT 1 cut(s) 1308
NspI RCATGY 3 cut(s) 478, 1011, 1310
OliI CACNNNNGTG 1 cut(s) 264
PaeI GCATGC 1 cut(s) 1310
PciI ACATGT 2 cut(s) 474, 1007
PdmI GAANNNNTTC 4 cut(s) 461, 764, 1068, 1398
PfeI GAWTC 5 cut(s) 161, 329, 710, 876, 1270
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 5 cut(s) 115, 643, 652, 862, 910
PpuMI RGGWCCY 2 cut(s) 94, 1358
PscI ACATGT 2 cut(s) 474, 1007
PshBI ATTAAT 1 cut(s) 791
Psp124BI GAGCTC 1 cut(s) 543
Psp5II RGGWCCY 2 cut(s) 94, 1358
Psp6I CCWGG 3 cut(s) 583, 813, 968
PspGI CCWGG 3 cut(s) 583, 813, 968
PspN4I GGNNCC 3 cut(s) 374, 956, 1425
PspPI GGNCC 4 cut(s) 94, 170, 527, 1358
PspPPI RGGWCCY 2 cut(s) 94, 1358
PsrI GAACNNNNNNTAC 2 cut(s) 365, 397
PstI CTGCAG 2 cut(s) 535, 655
PstNI CAGNNNCTG 1 cut(s) 814
PsuI RGATCY 2 cut(s) 810, 1396
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 4 cut(s) 315, 779, 956, 1343
RsaNI GTAC 4 cut(s) 314, 778, 955, 1342
RseI CAYNNNNRTG 2 cut(s) 264, 1432
SacI GAGCTC 1 cut(s) 543
SacII CCGCGG 1 cut(s) 912
SaqAI TTAA 5 cut(s) 90, 791, 851, 1214, 1527
SatI GCNGC 5 cut(s) 114, 642, 651, 861, 909
Sau96I GGNCC 4 cut(s) 94, 170, 527, 1358
SbfI CCTGCAGG 1 cut(s) 535
ScrFI CCNGG 4 cut(s) 377, 585, 815, 970
SdaI CCTGCAGG 1 cut(s) 535
SduI GDGCHC 2 cut(s) 543, 1057
SfaNI GCATC 7 cut(s) 21, 418, 963, 994, 1033, 1197, 1293
SfcI CTRYAG 3 cut(s) 531, 651, 963
Sfr303I CCGCGG 1 cut(s) 912
SgrBI CCGCGG 1 cut(s) 912
SinI GGWCC 3 cut(s) 94, 527, 1358
SmiMI CAYNNNNRTG 2 cut(s) 264, 1432
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SphI GCATGC 1 cut(s) 1310
Sse8387I CCTGCAGG 1 cut(s) 535
Sse9I AATT 6 cut(s) 236, 788, 848, 1000, 1211, 1498
SsiI CCGC 5 cut(s) 116, 429, 909, 911, 921
SspMI CTAG 6 cut(s) 392, 488, 857, 1134, 1176, 1355
SstI GAGCTC 1 cut(s) 543
StyD4I CCNGG 4 cut(s) 375, 583, 813, 968
StyI CCWWGG 3 cut(s) 799, 1354, 1433
TaaI ACNGT 4 cut(s) 55, 446, 1145, 1325
TaqI TCGA 3 cut(s) 164, 1373, 1388
TasI AATT 6 cut(s) 236, 788, 848, 1000, 1211, 1498
TatI WGTACW 1 cut(s) 1341
TauI GCSGC 1 cut(s) 911
TfiI GAWTC 5 cut(s) 161, 329, 710, 876, 1270
Tru1I TTAA 5 cut(s) 90, 791, 851, 1214, 1527
Tru9I TTAA 5 cut(s) 90, 791, 851, 1214, 1527
TscAI CASTG 4 cut(s) 608, 887, 1150, 1468
TseFI GTSAC 1 cut(s) 1228
TseI GCWGC 4 cut(s) 113, 641, 650, 860
Tsp45I GTSAC 1 cut(s) 1228
TspDTI ATGAA 4 cut(s) 470, 834, 906, 1266
TspGWI ACGGA 1 cut(s) 435
TspRI CASTG 4 cut(s) 608, 887, 1150, 1468
VneI GTGCAC 1 cut(s) 1053
VpaK11BI GGWCC 3 cut(s) 94, 527, 1358
VspI ATTAAT 1 cut(s) 791
XapI RAATTY 1 cut(s) 1000
XbaI TCTAGA 2 cut(s) 487, 1133
XceI RCATGY 3 cut(s) 478, 1011, 1310
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XmaJI CCTAGG 1 cut(s) 1354
XmnI GAANNNNTTC 4 cut(s) 461, 764, 1068, 1398
XspI CTAG 6 cut(s) 392, 488, 857, 1134, 1176, 1355
Zsp2I ATGCAT 1 cut(s) 1308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.