Rmu_sc0029272.1_g000002

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0029272.1
OG0006751
Physical Location & Seq
Forward (+)
4856 .. 5392
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0029272.1_g000002.1.cds

Sequence Viewer

Length: 456 bp
atgcatgtgcaggttttgagtggaggttggagagagaacatagaagttgcaggggaaaatgcacttgcaaggtatgatagtgcagcttatgaccaaatccttttaaatgctagaccaaatggcatcaacaaagatggccaaccaaaattaaggatgtatggggtgacttaccttcgtctggtggatgaactcctacaagaaacgaatttcaatatattcaagacatttgtaaagaagatgcatgctgatcaggattactgtcaagactcagagaagtacaaccaccgcctaggtcccttggaacgttcaaaaccaaagttctcaactgaagatcttctggatgcaacaaaaccagtggagccattcccgtgggataaagaaacagatatgagtgtcactggtggtggtggtctactttcttatttgataagcaaaatcttttctctatttaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

151

Amino Acids

17.24

Weight (kDa)

5.92

Isoelectric Point (pI)

54.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 412
AciI CCGC 1 cut(s) 286
AclI AACGTT 1 cut(s) 304
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 1 cut(s) 205
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 1 cut(s) 178
AgsI TTSAA 3 cut(s) 211, 220, 309
AluBI AGCT 1 cut(s) 86
AluI AGCT 1 cut(s) 86
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 1 cut(s) 83
ApoI RAATTY 1 cut(s) 205
Asp700I GAANNNNTTC 1 cut(s) 333
AspA2I CCTAGG 1 cut(s) 289
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 175
AvaII GGWCC 1 cut(s) 293
AvrII CCTAGG 1 cut(s) 289
BalI TGGCCA 1 cut(s) 138
BbvI GCAGC 1 cut(s) 95
BccI CCATC 1 cut(s) 128
BclI TGATCA 1 cut(s) 247
BfaI CTAG 2 cut(s) 111, 290
BglII AGATCT 1 cut(s) 331
BisI GCNGC 1 cut(s) 84
BlnI CCTAGG 1 cut(s) 289
BlsI GCNGC 1 cut(s) 85
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 295, 360
BmsI GCATC 3 cut(s) 132, 228, 331
BsaJI CCNNGG 3 cut(s) 289, 297, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse1I ACTGG 2 cut(s) 353, 403
BseDI CCNNGG 3 cut(s) 289, 297, 368
BseGI GGATG 3 cut(s) 159, 190, 346
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 2 cut(s) 353, 403
BseXI GCAGC 1 cut(s) 95
BsgI GTGCAG 2 cut(s) 29, 102
BshFI GGCC 1 cut(s) 138
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 1 cut(s) 178
BsmFI GGGAC 1 cut(s) 279
BsnI GGCC 1 cut(s) 138
Bsp143I GATC 2 cut(s) 247, 331
BspACI CCGC 1 cut(s) 286
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 1 cut(s) 281
BspLI GGNNCC 2 cut(s) 295, 360
BsrI ACTGG 2 cut(s) 353, 403
BssECI CCNNGG 3 cut(s) 289, 297, 368
BssMI GATC 2 cut(s) 247, 331
BssT1I CCWWGG 2 cut(s) 289, 297
Bst4CI ACNGT 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 1 cut(s) 268
BstDSI CCRYGG 1 cut(s) 368
BstF5I GGATG 3 cut(s) 159, 190, 346
BstKTI GATC 2 cut(s) 250, 334
BstMBI GATC 2 cut(s) 247, 331
BstNSI RCATGY 2 cut(s) 8, 245
BstV1I GCAGC 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 331
BstXI CCANNNNNNTGG 1 cut(s) 369
BstYI RGATCY 1 cut(s) 331
BsuRI GGCC 1 cut(s) 138
BtgI CCRYGG 1 cut(s) 368
BtsCI GGATG 3 cut(s) 159, 190, 346
BtsIMutI CAGTG 2 cut(s) 360, 396
Cac8I GCNNGC 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 293
Csp6I GTAC 1 cut(s) 277
CspCI CAANNNNNGTGG 2 cut(s) 336, 371
CviAII CATG 2 cut(s) 5, 242
CviJI RGCY 3 cut(s) 86, 138, 361
CviKI_1 RGCY 3 cut(s) 86, 138, 361
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 1 cut(s) 268
DpnI GATC 2 cut(s) 249, 333
DpnII GATC 2 cut(s) 247, 331
DraI TTTAAA 1 cut(s) 105
EaeI YGGCCR 1 cut(s) 136
Eco130I CCWWGG 2 cut(s) 289, 297
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 348
EcoO109I RGGNCCY 1 cut(s) 293
EcoT14I CCWWGG 2 cut(s) 289, 297
EcoT22I ATGCAT 2 cut(s) 6, 243
ErhI CCWWGG 2 cut(s) 289, 297
FaeI CATG 2 cut(s) 8, 245
FaiI YATR 8 cut(s) 6, 41, 75, 90, 159, 215, 243, 389
FaqI GGGAC 1 cut(s) 279
FatI CATG 2 cut(s) 4, 241
FbaI TGATCA 1 cut(s) 247
FblI GTMKAC 1 cut(s) 412
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 3 cut(s) 166, 197, 353
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 2 cut(s) 111, 290
GluI GCNGC 1 cut(s) 84
HaeIII GGCC 1 cut(s) 138
Hin1II CATG 2 cut(s) 8, 245
HinfI GANTC 1 cut(s) 266
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 413
Hpy188I TCNGA 1 cut(s) 271
Hpy188III TCNNGA 4 cut(s) 220, 251, 263, 338
Hpy8I GTNNAC 1 cut(s) 413
HpyAV CCTTC 1 cut(s) 182
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 8 cut(s) 4, 10, 50, 62, 68, 83, 241, 344
HpyF3I CTNAG 1 cut(s) 268
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 2 cut(s) 8, 245
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 2 cut(s) 247, 331
LmnI GCTCC 1 cut(s) 358
LpnPI CCDG 6 cut(s) 36, 164, 236, 323, 366, 384
Lsp1109I GCAGC 1 cut(s) 95
LweI GCATC 3 cut(s) 132, 228, 331
MaeI CTAG 2 cut(s) 111, 290
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 2 cut(s) 163, 394
MalI GATC 2 cut(s) 249, 333
MboI GATC 2 cut(s) 247, 331
MboII GAAGA 3 cut(s) 247, 326, 341
MflI RGATCY 1 cut(s) 331
MlsI TGGCCA 1 cut(s) 138
MluCI AATT 2 cut(s) 146, 205
MluNI TGGCCA 1 cut(s) 138
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 8
MnlI CCTC 1 cut(s) 17
Mox20I TGGCCA 1 cut(s) 138
Mph1103I ATGCAT 2 cut(s) 6, 243
MroXI GAANNNNTTC 1 cut(s) 333
MscI TGGCCA 1 cut(s) 138
MseI TTAA 3 cut(s) 104, 149, 450
MslI CAYNNNNRTG 1 cut(s) 367
Msp20I TGGCCA 1 cut(s) 138
NdeII GATC 2 cut(s) 247, 331
NlaIII CATG 2 cut(s) 8, 245
NlaIV GGNNCC 2 cut(s) 295, 360
NmuCI GTSAC 2 cut(s) 163, 394
NsiI ATGCAT 2 cut(s) 6, 243
NspI RCATGY 2 cut(s) 8, 245
PaeI GCATGC 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 333
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PpuMI RGGWCCY 1 cut(s) 293
Psp1406I AACGTT 1 cut(s) 304
Psp5II RGGWCCY 1 cut(s) 293
PspN4I GGNNCC 2 cut(s) 295, 360
PspPI GGNCC 1 cut(s) 293
PspPPI RGGWCCY 1 cut(s) 293
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
RseI CAYNNNNRTG 1 cut(s) 367
SaqAI TTAA 3 cut(s) 104, 149, 450
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 247, 331
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 1 cut(s) 260
SetI ASST 7 cut(s) 15, 28, 74, 88, 174, 295, 307
SfaNI GCATC 3 cut(s) 132, 228, 331
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 367
SphI GCATGC 1 cut(s) 245
Sse9I AATT 2 cut(s) 146, 205
SsiI CCGC 1 cut(s) 286
SspMI CTAG 2 cut(s) 111, 290
StyI CCWWGG 2 cut(s) 289, 297
TaaI ACNGT 1 cut(s) 260
TaiI ACGT 1 cut(s) 307
TasI AATT 2 cut(s) 146, 205
TatI WGTACW 1 cut(s) 276
Tru1I TTAA 3 cut(s) 104, 149, 450
Tru9I TTAA 3 cut(s) 104, 149, 450
TscAI CASTG 2 cut(s) 360, 403
TseFI GTSAC 2 cut(s) 163, 394
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 2 cut(s) 163, 394
TspDTI ATGAA 1 cut(s) 201
TspRI CASTG 2 cut(s) 360, 403
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 1 cut(s) 205
XceI RCATGY 2 cut(s) 8, 245
XmaJI CCTAGG 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 412
XmnI GAANNNNTTC 1 cut(s) 333
XspI CTAG 2 cut(s) 111, 290
Zsp2I ATGCAT 2 cut(s) 6, 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.