pycom09g02710

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006751
Physical Location & Seq
Reverse (-)
2008081 .. 2010206
2126 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g02710.1

Sequence Viewer

Length: 1023 bp
ATGACAACATTGACAAGAACAACAGCTCTAGGTGGTTTGGGGGAGAAAATAAGTGCTTACCACAGTAGGTGCTTCTTCTGCCCGCATTTTCCATTTTTAAACGTACTCGAGAGTACAGAGGGAGCAAGTAAAGTATGGAGGCAAGGAGTCAATTCAGCTCCGGCATTCTGCTCCCGCAGTGCAATCCAAAGTCCTCGAGCTATTGCATCTGAAATCTCCACCACAGAACGAAAGGTTGCAACCACTCCCACTTATCAAGAAAAAATGCTCAAAAACTATGTGCCGATCTTCGTGATGCTCCCTTTGGGAGTTGTTACGGTTGACAATGTTTTGGAAGACGGGGACAAACTCGTTAAGGATCTGAAGGAGTTACGGGCAGCGGGTGTTGATGGGGTCATGATAGATGTGTGGTGGGGGATCATAGAATCAAAGGGGCCTAAGCAGTATGACTGGAGTGCTTATAGGAGCGTGTTTCAAGCAGTTCAAGATTGTGGGCTGAAATTACAAGCTATAATGTCATTCCACCAATGTGGTGGGAATGTAGGAGATGTTGTAACCATCCCCATTCCCAAATGGGTACTTGAGATCGGAGAATCAGACCCCGATATCTTTTATACCAATCTCAAAGGTAATAGGAACCCGGAATACCTCACTCTAGGTGTGGATAATCTGCATCTCTTTGACGGACGAACTGCTGTGCAGATATACAGTGATTACATGAAAAGTTTCAGAGAGAACATGTCGGATTTTCTACAAACTGGACTTATTATAGACATTGAAGTAGGGCTTGGACCGGCAGGAGAGCTCAGGTATCCCTCTTATCCGGAAAGTCAAGGATGGGCTTTTCCAGGGATGGGAGAATTTCAGTGCTATGACAAGTATCTCCAAGCAGAGTTCAAAGAGGCTGCAACAGCTGCAGGTCATCCTGAATGGGCTTTGCCAGATAATGCCGGAGAATATAATGACGCTCCTGAATCCTCAGAGTTCTTCAGATCGATCTTGAAATGGGACATATGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

341

Amino Acids

37.96

Weight (kDa)

4.91

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 97 - 336 1.1e-82 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 908
AccIII TCCGGA 1 cut(s) 823
AciI CCGC 3 cut(s) 83, 175, 380
AclWI GGATC 2 cut(s) 366, 425
AcsI RAATTY 1 cut(s) 860
AcuI CTGAAG 2 cut(s) 383, 973
AfaI GTAC 3 cut(s) 105, 115, 579
AfiI CCNNNNNNNGG 1 cut(s) 854
AflIII ACRYGT 1 cut(s) 738
AgsI TTSAA 5 cut(s) 476, 485, 779, 898, 1003
AjnI CCWGG 1 cut(s) 847
AjuI GAANNNNNNNTTGG 2 cut(s) 771, 803
AleI CACNNNNGTG 1 cut(s) 528
AluBI AGCT 6 cut(s) 26, 158, 200, 509, 805, 914
AluI AGCT 6 cut(s) 26, 158, 200, 509, 805, 914
Alw21I GWGCWC 1 cut(s) 807
AlwI GGATC 2 cut(s) 366, 425
Ama87I CYCGRG 2 cut(s) 107, 195
Aor13HI TCCGGA 1 cut(s) 823
AoxI GGCC 1 cut(s) 434
ApeKI GCWGC 3 cut(s) 377, 905, 914
ApoI RAATTY 1 cut(s) 860
Asp700I GAANNNNTTC 1 cut(s) 725
AspS9I GGNCC 2 cut(s) 434, 791
AsuC2I CCSGG 1 cut(s) 641
AvaI CYCGRG 2 cut(s) 107, 195
AvaII GGWCC 1 cut(s) 791
BanII GRGCYC 1 cut(s) 807
BbsI GAAGAC 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 807
BbvI GCAGC 3 cut(s) 389, 892, 901
BccI CCATC 4 cut(s) 383, 566, 831, 847
BciT130I CCWGG 1 cut(s) 849
BciVI GTATCC 1 cut(s) 822
BcnI CCSGG 1 cut(s) 641
BfaI CTAG 2 cut(s) 29, 656
BfmI CTRYAG 1 cut(s) 915
BfuAI ACCTGC 1 cut(s) 908
BfuI GTATCC 1 cut(s) 822
BisI GCNGC 3 cut(s) 378, 906, 915
BlsI GCNGC 3 cut(s) 379, 907, 916
Bme1390I CCNGG 2 cut(s) 641, 849
Bme18I GGWCC 1 cut(s) 791
BmeT110I CYCGRG 2 cut(s) 107, 195
BmgT120I GGNCC 2 cut(s) 434, 791
BmiI GGNNCC 2 cut(s) 435, 638
BmrFI CCNGG 2 cut(s) 641, 849
BmsI GCATC 3 cut(s) 215, 285, 682
BpiI GAAGAC 1 cut(s) 342
BpmI CTGGAG 1 cut(s) 472
Bpu10I CCTNAGC 2 cut(s) 438, 806
BpuEI CTTGAG 1 cut(s) 602
BpuMI CCSGG 1 cut(s) 641
Bsa29I ATCGAT 1 cut(s) 995
BsaJI CCNNGG 1 cut(s) 848
BsaWI WCCGGW 1 cut(s) 823
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 1 cut(s) 854
Bse118I RCCGGY 1 cut(s) 793
Bse1I ACTGG 2 cut(s) 455, 763
BseAI TCCGGA 1 cut(s) 823
BseBI CCWGG 1 cut(s) 849
BseCI ATCGAT 1 cut(s) 995
BseDI CCNNGG 1 cut(s) 848
BseGI GGATG 4 cut(s) 558, 842, 858, 922
BseLI CCNNNNNNNGG 1 cut(s) 854
BseMII CTCAG 2 cut(s) 820, 993
BseNI ACTGG 2 cut(s) 455, 763
BseXI GCAGC 3 cut(s) 389, 892, 901
BsgI GTGCAG 1 cut(s) 719
BshFI GGCC 1 cut(s) 436
BshVI ATCGAT 1 cut(s) 995
BsiHKAI GWGCWC 1 cut(s) 807
BsiHKCI CYCGRG 2 cut(s) 107, 195
BsiSI CCGG 5 cut(s) 161, 641, 794, 824, 951
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 854
BsmFI GGGAC 1 cut(s) 356
BsmI GAATGC 1 cut(s) 164
BsnI GGCC 1 cut(s) 436
BsoBI CYCGRG 2 cut(s) 107, 195
Bsp1286I GDGCHC 1 cut(s) 807
Bsp13I TCCGGA 1 cut(s) 823
Bsp143I GATC 6 cut(s) 285, 358, 417, 585, 992, 996
BspACI CCGC 3 cut(s) 83, 175, 380
BspANI GGCC 1 cut(s) 436
BspCNI CTCAG 2 cut(s) 819, 992
BspDI ATCGAT 1 cut(s) 995
BspEI TCCGGA 1 cut(s) 823
BspHI TCATGA 1 cut(s) 396
BspLI GGNNCC 2 cut(s) 435, 638
BspMAI CTGCAG 1 cut(s) 919
BspMI ACCTGC 1 cut(s) 908
BspPI GGATC 2 cut(s) 366, 425
BsrFI RCCGGY 1 cut(s) 793
BsrI ACTGG 2 cut(s) 455, 763
BssAI RCCGGY 1 cut(s) 793
BssECI CCNNGG 1 cut(s) 848
BssMI GATC 6 cut(s) 285, 358, 417, 585, 992, 996
Bst2UI CCWGG 1 cut(s) 849
Bst4CI ACNGT 3 cut(s) 65, 319, 710
BstAPI GCANNNNNTGC 1 cut(s) 914
BstC8I GCNNGC 1 cut(s) 83
BstDEI CTNAG 3 cut(s) 438, 806, 979
BstF5I GGATG 4 cut(s) 558, 842, 858, 922
BstKTI GATC 6 cut(s) 288, 361, 420, 588, 995, 999
BstMBI GATC 6 cut(s) 285, 358, 417, 585, 992, 996
BstMWI GCNNNNNNNGC 3 cut(s) 78, 911, 914
BstNI CCWGG 1 cut(s) 849
BstNSI RCATGY 1 cut(s) 742
BstSCI CCNGG 2 cut(s) 639, 847
BstSFI CTRYAG 1 cut(s) 915
BstV1I GCAGC 3 cut(s) 389, 892, 901
BstV2I GAAGAC 1 cut(s) 342
BstX2I RGATCY 1 cut(s) 358
BstXI CCANNNNNNTGG 2 cut(s) 530, 533
BstYI RGATCY 1 cut(s) 358
Bsu15I ATCGAT 1 cut(s) 995
BsuI GTATCC 1 cut(s) 822
BsuRI GGCC 1 cut(s) 436
BsuTUI ATCGAT 1 cut(s) 995
BtsCI GGATG 4 cut(s) 558, 842, 858, 922
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 3 cut(s) 184, 715, 872
BveI ACCTGC 1 cut(s) 908
Cac8I GCNNGC 1 cut(s) 83
CciI TCATGA 1 cut(s) 396
Cfr10I RCCGGY 1 cut(s) 793
Cfr13I GGNCC 2 cut(s) 434, 791
ClaI ATCGAT 1 cut(s) 995
CseI GACGC 1 cut(s) 974
Csp6I GTAC 3 cut(s) 104, 114, 578
CviAII CATG 3 cut(s) 397, 718, 739
CviQI GTAC 3 cut(s) 104, 114, 578
DdeI CTNAG 3 cut(s) 438, 806, 979
DpnI GATC 6 cut(s) 287, 360, 419, 587, 994, 998
DpnII GATC 6 cut(s) 285, 358, 417, 585, 992, 996
DraI TTTAAA 1 cut(s) 99
Ecl136II GAGCTC 1 cut(s) 805
Eco24I GRGCYC 1 cut(s) 807
Eco32I GATATC 1 cut(s) 607
Eco47I GGWCC 1 cut(s) 791
Eco53kI GAGCTC 1 cut(s) 805
Eco57I CTGAAG 2 cut(s) 383, 973
Eco88I CYCGRG 2 cut(s) 107, 195
EcoICRI GAGCTC 1 cut(s) 805
EcoO109I RGGNCCY 1 cut(s) 434
EcoRII CCWGG 1 cut(s) 847
EcoRV GATATC 1 cut(s) 607
EcoT38I GRGCYC 1 cut(s) 807
FaeI CATG 3 cut(s) 400, 721, 742
FalI AAGNNNNNCTT 2 cut(s) 771, 803
FaqI GGGAC 1 cut(s) 356
FatI CATG 3 cut(s) 396, 717, 738
FauI CCCGC 3 cut(s) 90, 182, 373
FauNDI CATATG 1 cut(s) 1013
Fnu4HI GCNGC 3 cut(s) 378, 906, 915
FokI GGATG 4 cut(s) 545, 849, 865, 909
FriOI GRGCYC 1 cut(s) 807
Fsp4HI GCNGC 3 cut(s) 378, 906, 915
FspBI CTAG 2 cut(s) 29, 656
GluI GCNGC 3 cut(s) 378, 906, 915
GsuI CTGGAG 1 cut(s) 472
HaeIII GGCC 1 cut(s) 436
HapII CCGG 5 cut(s) 161, 641, 794, 824, 951
HgaI GACGC 1 cut(s) 974
Hin1II CATG 3 cut(s) 400, 721, 742
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HinfI GANTC 4 cut(s) 147, 425, 593, 974
HpaII CCGG 5 cut(s) 161, 641, 794, 824, 951
Hpy166II GTNNAC 1 cut(s) 322
Hpy188I TCNGA 8 cut(s) 211, 363, 590, 598, 731, 745, 982, 992
Hpy188III TCNNGA 9 cut(s) 109, 257, 292, 397, 485, 824, 926, 971, 1000
Hpy8I GTNNAC 1 cut(s) 322
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 3 cut(s) 65, 319, 710
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 7 cut(s) 182, 206, 239, 673, 700, 908, 917
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 911, 914
HpyF3I CTNAG 3 cut(s) 438, 806, 979
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 3 cut(s) 400, 721, 742
Kpn2I TCCGGA 1 cut(s) 823
Kzo9I GATC 6 cut(s) 285, 358, 417, 585, 992, 996
LmnI GCTCC 6 cut(s) 122, 163, 176, 303, 465, 973
Lsp1109I GCAGC 3 cut(s) 389, 892, 901
LweI GCATC 3 cut(s) 215, 285, 682
MaeI CTAG 2 cut(s) 29, 656
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 4 cut(s) 313, 369, 553, 1016
MalI GATC 6 cut(s) 287, 360, 419, 587, 994, 998
MboI GATC 6 cut(s) 285, 358, 417, 585, 992, 996
MboII GAAGA 4 cut(s) 67, 280, 347, 979
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 807
MluCI AATT 3 cut(s) 151, 500, 860
MlyI GAGTC 1 cut(s) 156
MmeI TCCRAC 1 cut(s) 723
MnlI CCTC 7 cut(s) 112, 132, 204, 659, 826, 895, 988
MroI TCCGGA 1 cut(s) 823
MroXI GAANNNNTTC 1 cut(s) 725
MseI TTAA 2 cut(s) 98, 354
MslI CAYNNNNRTG 1 cut(s) 528
MspA1I CMGCKG 2 cut(s) 380, 914
MspI CCGG 5 cut(s) 161, 641, 794, 824, 951
MspR9I CCNGG 2 cut(s) 641, 849
Mva1269I GAATGC 1 cut(s) 164
MvaI CCWGG 1 cut(s) 849
MwoI GCNNNNNNNGC 3 cut(s) 78, 911, 914
NciI CCSGG 1 cut(s) 641
NdeI CATATG 1 cut(s) 1013
NdeII GATC 6 cut(s) 285, 358, 417, 585, 992, 996
NlaIII CATG 3 cut(s) 400, 721, 742
NlaIV GGNNCC 2 cut(s) 435, 638
NspI RCATGY 1 cut(s) 742
OliI CACNNNNGTG 1 cut(s) 528
PaeR7I CTCGAG 2 cut(s) 107, 195
PagI TCATGA 1 cut(s) 396
PciI ACATGT 1 cut(s) 738
PctI GAATGC 1 cut(s) 164
PdmI GAANNNNTTC 1 cut(s) 725
PfeI GAWTC 3 cut(s) 425, 593, 974
PkrI GCNGC 3 cut(s) 379, 907, 916
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PscI ACATGT 1 cut(s) 738
Psp124BI GAGCTC 1 cut(s) 807
Psp6I CCWGG 1 cut(s) 847
PspGI CCWGG 1 cut(s) 847
PspN4I GGNNCC 2 cut(s) 435, 638
PspPI GGNCC 2 cut(s) 434, 791
PspXI VCTCGAGB 1 cut(s) 195
PsrI GAACNNNNNNTAC 2 cut(s) 629, 661
PstI CTGCAG 1 cut(s) 919
PsuI RGATCY 1 cut(s) 358
PvuII CAGCTG 1 cut(s) 914
RsaI GTAC 3 cut(s) 105, 115, 579
RsaNI GTAC 3 cut(s) 104, 114, 578
RseI CAYNNNNRTG 1 cut(s) 528
SacI GAGCTC 1 cut(s) 807
SaqAI TTAA 2 cut(s) 98, 354
SatI GCNGC 3 cut(s) 378, 906, 915
Sau3AI GATC 6 cut(s) 285, 358, 417, 585, 992, 996
Sau96I GGNCC 2 cut(s) 434, 791
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 2 cut(s) 641, 849
SduI GDGCHC 1 cut(s) 807
SfaNI GCATC 3 cut(s) 215, 285, 682
SfcI CTRYAG 1 cut(s) 915
Sfr274I CTCGAG 2 cut(s) 107, 195
SinI GGWCC 1 cut(s) 791
SlaI CTCGAG 2 cut(s) 107, 195
SmiMI CAYNNNNRTG 1 cut(s) 528
SmlI CTYRAG 3 cut(s) 107, 195, 581
SmoI CTYRAG 3 cut(s) 107, 195, 581
Sse9I AATT 3 cut(s) 151, 500, 860
SsiI CCGC 3 cut(s) 83, 175, 380
SspMI CTAG 2 cut(s) 29, 656
SstI GAGCTC 1 cut(s) 807
StyD4I CCNGG 2 cut(s) 639, 847
TaaI ACNGT 3 cut(s) 65, 319, 710
TaiI ACGT 1 cut(s) 105
TaqI TCGA 3 cut(s) 108, 196, 995
TasI AATT 3 cut(s) 151, 500, 860
TatI WGTACW 1 cut(s) 113
TfiI GAWTC 3 cut(s) 425, 593, 974
Tru1I TTAA 2 cut(s) 98, 354
Tru9I TTAA 2 cut(s) 98, 354
TscAI CASTG 3 cut(s) 184, 715, 872
TseI GCWGC 3 cut(s) 377, 905, 914
TspDTI ATGAA 1 cut(s) 734
TspGWI ACGGA 1 cut(s) 699
TspRI CASTG 3 cut(s) 184, 715, 872
VpaK11BI GGWCC 1 cut(s) 791
XapI RAATTY 1 cut(s) 860
XceI RCATGY 1 cut(s) 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 530
XhoI CTCGAG 2 cut(s) 107, 195
XmnI GAANNNNTTC 1 cut(s) 725
XspI CTAG 2 cut(s) 29, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.