Rorug02G0482700

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006751
Physical Location & Seq
Reverse (-)
61269268 .. 61270614
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0482700.1

Sequence Viewer

Length: 642 bp
ATGTCATTGGTACTTGGTGCAAAGCGCTACCTGGAATGGAGGCATGTGAAATACATCATGAGCAGAATCTCAGAGGATCCTGTACTGGCTGCCCTGGGAGGGGAGGTTGTAAACTTGAAACAAATCCGCGCATTCCTCAGGGTGCATTTAAAAGATTATGGAGTTCTAGATTTTGATGCAGGTGATGCTTGTAGTCCGCCTCCTGTCGATACCACTTGGCAGCAGATTTACTTTTGCATGAGAGCTGGGTATTATGATGAAGCAAGAACCGTTGCTTCATCATCAAATGCTTCACAGGAGTTTGCGCCTCTGCTCAATGAGTGGATTACATCTGGAGGGATGGTGTCTGTGGAGATTGCAGCCACAGCTTCTGATGAATTGGATATGATGTTAAGAACAGTTAATGTTGAGGGTGGAGCTGGATATGAGAAGGAAAAGTTGTTACTATATTCTCTCATATCTGGTTCAAGAAGCCTTTTTGACAGCCTGCTAGAAGATCAGCCAACTCTATTCGACACTGAAGAGGATTTCTACTGGTTTAGATTATCATCTATGAGAGAATGCCCAGTTGGAGCTGCATCCACTGTTATGAATGCTGGCTCGGAACCATACACATTGAAGGATTTGCAAGTCTGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

213

Amino Acids

23.77

Weight (kDa)

4.58

Isoelectric Point (pI)

48.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nic96 PF04097 2 - 210 4.6e-29 Nup93/Nic96
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 170
AccII CGCG 1 cut(s) 129
AciI CCGC 2 cut(s) 127, 197
AclWI GGATC 2 cut(s) 71, 84
AcuI CTGAAG 1 cut(s) 540
AfaI GTAC 2 cut(s) 12, 84
AfeI AGCGCT 1 cut(s) 26
AfiI CCNNNNNNNGG 2 cut(s) 99, 100
AgsI TTSAA 3 cut(s) 118, 468, 619
AjnI CCWGG 2 cut(s) 30, 93
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AluBI AGCT 4 cut(s) 245, 368, 419, 575
AluI AGCT 4 cut(s) 245, 368, 419, 575
AlwI GGATC 2 cut(s) 71, 84
AlwNI CAGNNNCTG 1 cut(s) 371
Aor51HI AGCGCT 1 cut(s) 26
ApeKI GCWGC 4 cut(s) 89, 220, 359, 575
AspLEI GCGC 3 cut(s) 27, 131, 307
AsuHPI GGTGA 1 cut(s) 194
AxyI CCTNAGG 1 cut(s) 137
BamHI GGATCC 1 cut(s) 76
BbvI GCAGC 4 cut(s) 76, 232, 371, 562
BccI CCATC 1 cut(s) 334
BciT130I CCWGG 2 cut(s) 32, 95
BfaI CTAG 2 cut(s) 167, 491
BfoI RGCGCY 1 cut(s) 28
BfuAI ACCTGC 1 cut(s) 170
BisI GCNGC 4 cut(s) 90, 221, 360, 576
BlsI GCNGC 4 cut(s) 91, 222, 361, 577
Bme1390I CCNGG 2 cut(s) 32, 95
BmiI GGNNCC 2 cut(s) 78, 606
BmrFI CCNGG 2 cut(s) 32, 95
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 3 cut(s) 166, 175, 587
BmuI ACTGGG 1 cut(s) 560
BpmI CTGGAG 1 cut(s) 354
BsaBI GATNNNNATC 1 cut(s) 547
BsaJI CCNNGG 2 cut(s) 93, 94
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 327, 357
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 100
Bse1I ACTGG 3 cut(s) 90, 539, 566
Bse21I CCTNAGG 1 cut(s) 137
Bse8I GATNNNNATC 1 cut(s) 547
BseBI CCWGG 2 cut(s) 32, 95
BseDI CCNNGG 2 cut(s) 93, 94
BseGI GGATG 2 cut(s) 345, 578
BseJI GATNNNNATC 1 cut(s) 547
BseLI CCNNNNNNNGG 2 cut(s) 99, 100
BseMII CTCAG 2 cut(s) 84, 151
BseNI ACTGG 3 cut(s) 90, 539, 566
BseXI GCAGC 4 cut(s) 76, 232, 371, 562
BseYI CCCAGC 1 cut(s) 245
Bsh1236I CGCG 1 cut(s) 129
BslI CCNNNNNNNGG 2 cut(s) 99, 100
BsmI GAATGC 3 cut(s) 131, 566, 598
Bsp143I GATC 2 cut(s) 76, 496
BspACI CCGC 2 cut(s) 127, 197
BspCNI CTCAG 2 cut(s) 83, 150
BspFNI CGCG 1 cut(s) 129
BspHI TCATGA 1 cut(s) 57
BspLI GGNNCC 2 cut(s) 78, 606
BspMI ACCTGC 1 cut(s) 170
BspPI GGATC 2 cut(s) 71, 84
BsrI ACTGG 3 cut(s) 90, 539, 566
BssECI CCNNGG 2 cut(s) 93, 94
BssMI GATC 2 cut(s) 76, 496
Bst2UI CCWGG 2 cut(s) 32, 95
Bst4CI ACNGT 3 cut(s) 271, 400, 586
Bst6I CTCTTC 1 cut(s) 516
BstAPI GCANNNNNTGC 1 cut(s) 185
BstC8I GCNNGC 2 cut(s) 488, 598
BstDEI CTNAG 2 cut(s) 70, 137
BstF5I GGATG 2 cut(s) 345, 578
BstFNI CGCG 1 cut(s) 129
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 3 cut(s) 27, 131, 307
BstKTI GATC 2 cut(s) 79, 499
BstMBI GATC 2 cut(s) 76, 496
BstMWI GCNNNNNNNGC 2 cut(s) 185, 365
BstNI CCWGG 2 cut(s) 32, 95
BstNSI RCATGY 1 cut(s) 47
BstSCI CCNGG 2 cut(s) 30, 93
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 4 cut(s) 76, 232, 371, 562
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
Bsu36I CCTNAGG 1 cut(s) 137
BtsCI GGATG 2 cut(s) 345, 578
BtsIMutI CAGTG 2 cut(s) 516, 582
BveI ACCTGC 1 cut(s) 170
Cac8I GCNNGC 2 cut(s) 488, 598
CaiI CAGNNNCTG 1 cut(s) 371
CciI TCATGA 1 cut(s) 57
CfoI GCGC 3 cut(s) 27, 131, 307
Csp6I GTAC 2 cut(s) 11, 83
CviAII CATG 3 cut(s) 44, 58, 238
CviQI GTAC 2 cut(s) 11, 83
DdeI CTNAG 2 cut(s) 70, 137
DpnI GATC 2 cut(s) 78, 498
DpnII GATC 2 cut(s) 76, 496
DraI TTTAAA 1 cut(s) 150
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
EciI GGCGGA 1 cut(s) 186
Eco47III AGCGCT 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 540
Eco81I CCTNAGG 1 cut(s) 137
EcoRII CCWGG 2 cut(s) 30, 93
FaeI CATG 3 cut(s) 47, 61, 241
FatI CATG 3 cut(s) 43, 57, 237
Fnu4HI GCNGC 4 cut(s) 90, 221, 360, 576
FokI GGATG 2 cut(s) 352, 565
Fsp4HI GCNGC 4 cut(s) 90, 221, 360, 576
FspBI CTAG 2 cut(s) 167, 491
GlaI GCGC 3 cut(s) 26, 130, 306
GluI GCNGC 4 cut(s) 90, 221, 360, 576
GsaI CCCAGC 1 cut(s) 249
GsuI CTGGAG 1 cut(s) 354
HaeII RGCGCY 1 cut(s) 28
HhaI GCGC 3 cut(s) 27, 131, 307
Hin1II CATG 3 cut(s) 47, 61, 241
Hin6I GCGC 3 cut(s) 25, 129, 305
HinP1I GCGC 3 cut(s) 25, 129, 305
HinfI GANTC 1 cut(s) 66
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 3 cut(s) 73, 373, 604
Hpy188III TCNNGA 4 cut(s) 58, 167, 333, 468
Hpy8I GTNNAC 1 cut(s) 112
HpyAV CCTTC 2 cut(s) 424, 613
HpyCH4III ACNGT 3 cut(s) 271, 400, 586
HpyCH4V TGCA 7 cut(s) 20, 145, 179, 237, 359, 578, 628
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 365
HpyF3I CTNAG 2 cut(s) 70, 137
Hsp92II CATG 3 cut(s) 47, 61, 241
HspAI GCGC 3 cut(s) 25, 129, 305
Kzo9I GATC 2 cut(s) 76, 496
LmnI GCTCC 2 cut(s) 416, 572
Lsp1109I GCAGC 4 cut(s) 76, 232, 371, 562
LweI GCATC 3 cut(s) 166, 175, 587
MaeI CTAG 2 cut(s) 167, 491
MaeIII GTNAC 1 cut(s) 441
MalI GATC 2 cut(s) 78, 498
MboI GATC 2 cut(s) 76, 496
MboII GAAGA 2 cut(s) 506, 533
MflI RGATCY 1 cut(s) 76
MluCI AATT 1 cut(s) 377
MmeI TCCRAC 1 cut(s) 550
MseI TTAA 3 cut(s) 149, 392, 402
MslI CAYNNNNRTG 1 cut(s) 587
MspR9I CCNGG 2 cut(s) 32, 95
Mva1269I GAATGC 3 cut(s) 131, 566, 598
MvaI CCWGG 2 cut(s) 32, 95
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 185, 365
NdeII GATC 2 cut(s) 76, 496
NlaIII CATG 3 cut(s) 47, 61, 241
NlaIV GGNNCC 2 cut(s) 78, 606
NspI RCATGY 1 cut(s) 47
PagI TCATGA 1 cut(s) 57
PaqCI CACCTGC 1 cut(s) 170
PasI CCCWGGG 1 cut(s) 94
PctI GAATGC 3 cut(s) 131, 566, 598
PfeI GAWTC 1 cut(s) 66
PkrI GCNGC 4 cut(s) 91, 222, 361, 577
Psp6I CCWGG 2 cut(s) 30, 93
PspFI CCCAGC 1 cut(s) 245
PspGI CCWGG 2 cut(s) 30, 93
PspN4I GGNNCC 2 cut(s) 78, 606
PstNI CAGNNNCTG 1 cut(s) 371
PsuI RGATCY 1 cut(s) 76
RsaI GTAC 2 cut(s) 12, 84
RsaNI GTAC 2 cut(s) 11, 83
RseI CAYNNNNRTG 1 cut(s) 587
SaqAI TTAA 3 cut(s) 149, 392, 402
SatI GCNGC 4 cut(s) 90, 221, 360, 576
Sau3AI GATC 2 cut(s) 76, 496
ScrFI CCNGG 2 cut(s) 32, 95
SetI ASST 7 cut(s) 33, 108, 184, 247, 370, 421, 577
SfaNI GCATC 3 cut(s) 166, 175, 587
SmiMI CAYNNNNRTG 1 cut(s) 587
Sse9I AATT 1 cut(s) 377
SsiI CCGC 2 cut(s) 127, 197
SspMI CTAG 2 cut(s) 167, 491
StyD4I CCNGG 2 cut(s) 30, 93
TaaI ACNGT 3 cut(s) 271, 400, 586
TaqI TCGA 2 cut(s) 207, 513
TasI AATT 1 cut(s) 377
TatI WGTACW 1 cut(s) 82
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 3 cut(s) 149, 392, 402
Tru9I TTAA 3 cut(s) 149, 392, 402
TscAI CASTG 2 cut(s) 523, 589
TseI GCWGC 4 cut(s) 89, 220, 359, 575
TspDTI ATGAA 4 cut(s) 267, 273, 390, 605
TspRI CASTG 2 cut(s) 523, 589
XbaI TCTAGA 1 cut(s) 166
XceI RCATGY 1 cut(s) 47
XspI CTAG 2 cut(s) 167, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.