Rorug02G0482800

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006751
Physical Location & Seq
Forward (+)
61277843 .. 61278607
765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0482800.1

Sequence Viewer

Length: 765 bp
ATGTCAGAGCTATCACGACTTTCAATGACAAACAATAACCGCAGAGGTCGTCGCAGTGAGGAGTATGAGCCTAGGTTCTCAAACCTTCCACCACTCCCACTACTGCGTGTCAATTACGGCCGGGGTCATCGCAGTGAGGAGTCGCCTAGGATTTCAGAGCTTCCACAATTGACACAAACAAACAATAACCGTGGTCGTCGTCACTGGCAGCAGCAGCCTGCTTGGATCTCTGACCTTCCAGGCTGCGACCGGTGTGTGACTATGAACCTGTCGTGCTCCGAGTGTTGGGAACGTGATCATTTGCGAACATTATTGGAGGACGAGGAGCTCTTTGATCGTGAAATGCAGGAAATGCGAGACTCTTACAGTCGAGAACAGGAAGAAGCTATTCGAAGAACAAGGTCGGCGTCAACAGAGACAAGAGAACAGGAGGAAGCTATGCAAAGAACAAGGTCGGCGTCAACAGCGACAAGACACAGTATGTTTGACAGATTGGTGGAACTAGAATTTCAGATGTTTAGGTCAAAAACTACAAGAATTGATGATGTGAAAGCCAAGGTCAGGGGCATGCCAACCATTATGGCAATTGATGAGGAAGCTTGTGCAGTTTGTCTCGAAGGCTTTCATTGTGTGTTTAAAGATGACGAGGGTAGTACTGGGAAACAAACACCCTGTGGCCATTTCTTCCACCAAACTTGCATAGCAAAATGGCTTTCAAACCCAAAGTACAGTTCTACCTGCCCTCTATGTCGCTACTCATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

254

Amino Acids

29.77

Weight (kDa)

6.67

Isoelectric Point (pI)

65.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 200 - 251 3.3e-09 Ring finger domain
zf-rbx1 PF12678 200 - 251 2.9e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 201 - 250 2.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 209 - 253 2e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 746
AciI CCGC 1 cut(s) 40
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 2 cut(s) 118, 676
AcsI RAATTY 1 cut(s) 506
AcyI GRCGYC 2 cut(s) 407, 458
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 2 cut(s) 655, 728
AfiI CCNNNNNNNGG 2 cut(s) 285, 561
AgeI ACCGGT 1 cut(s) 249
AgsI TTSAA 2 cut(s) 24, 717
AjnI CCWGG 1 cut(s) 238
AjuI GAANNNNNNNTTGG 2 cut(s) 715, 747
AluBI AGCT 6 cut(s) 10, 160, 328, 386, 437, 599
AluI AGCT 6 cut(s) 10, 160, 328, 386, 437, 599
Alw21I GWGCWC 2 cut(s) 278, 330
Alw26I GTCTC 3 cut(s) 351, 410, 617
AlwI GGATC 1 cut(s) 233
AoxI GGCC 2 cut(s) 118, 676
ApeKI GCWGC 4 cut(s) 208, 211, 214, 243
ApoI RAATTY 1 cut(s) 506
AsiGI ACCGGT 1 cut(s) 249
Asp700I GAANNNNTTC 2 cut(s) 387, 621
AspA2I CCTAGG 2 cut(s) 71, 146
AsuC2I CCSGG 1 cut(s) 122
AsuII TTCGAA 1 cut(s) 391
AvrII CCTAGG 2 cut(s) 71, 146
BalI TGGCCA 1 cut(s) 678
BanII GRGCYC 1 cut(s) 330
Bbv12I GWGCWC 2 cut(s) 278, 330
BbvI GCAGC 4 cut(s) 220, 223, 226, 230
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 1 cut(s) 240
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 3 cut(s) 351, 410, 617
BfaI CTAG 3 cut(s) 72, 147, 503
BfuAI ACCTGC 1 cut(s) 746
BisI GCNGC 4 cut(s) 209, 212, 215, 244
BlnI CCTAGG 2 cut(s) 71, 146
BlsI GCNGC 4 cut(s) 210, 213, 216, 245
BmcAI AGTACT 1 cut(s) 655
Bme1390I CCNGG 2 cut(s) 122, 240
BmrFI CCNGG 2 cut(s) 122, 240
BmrI ACTGGG 1 cut(s) 666
BmuI ACTGGG 1 cut(s) 666
Bpu14I TTCGAA 1 cut(s) 391
BpuMI CCSGG 1 cut(s) 122
BsaHI GRCGYC 2 cut(s) 407, 458
BsaJI CCNNGG 5 cut(s) 71, 121, 146, 190, 555
BsaWI WCCGGW 1 cut(s) 249
Bsc4I CCNNNNNNNGG 2 cut(s) 285, 561
Bse118I RCCGGY 1 cut(s) 249
Bse1I ACTGG 2 cut(s) 209, 661
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 5 cut(s) 71, 121, 146, 190, 555
BseLI CCNNNNNNNGG 2 cut(s) 285, 561
BseNI ACTGG 2 cut(s) 209, 661
BseRI GAGGAG 3 cut(s) 74, 152, 338
BseX3I CGGCCG 1 cut(s) 118
BseXI GCAGC 4 cut(s) 220, 223, 226, 230
BsgI GTGCAG 1 cut(s) 624
Bsh1285I CGRYCG 2 cut(s) 121, 250
BshFI GGCC 2 cut(s) 120, 678
BshTI ACCGGT 1 cut(s) 249
BsiEI CGRYCG 2 cut(s) 121, 250
BsiHKAI GWGCWC 2 cut(s) 278, 330
BsiSI CCGG 2 cut(s) 121, 250
BslI CCNNNNNNNGG 2 cut(s) 285, 561
BsmAI GTCTC 3 cut(s) 351, 410, 617
BsnI GGCC 2 cut(s) 120, 678
Bsp119I TTCGAA 1 cut(s) 391
Bsp1286I GDGCHC 2 cut(s) 278, 330
Bsp143I GATC 3 cut(s) 225, 295, 334
BspACI CCGC 1 cut(s) 40
BspANI GGCC 2 cut(s) 120, 678
BspMI ACCTGC 1 cut(s) 746
BspPI GGATC 1 cut(s) 233
BspT104I TTCGAA 1 cut(s) 391
BsrFI RCCGGY 1 cut(s) 249
BsrI ACTGG 2 cut(s) 209, 661
BssAI RCCGGY 1 cut(s) 249
BssECI CCNNGG 5 cut(s) 71, 121, 146, 190, 555
BssMI GATC 3 cut(s) 225, 295, 334
BssNI GRCGYC 2 cut(s) 407, 458
BssT1I CCWWGG 3 cut(s) 71, 146, 555
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 4 cut(s) 191, 368, 479, 731
BstACI GRCGYC 2 cut(s) 407, 458
BstAPI GCANNNNNTGC 1 cut(s) 352
BstBI TTCGAA 1 cut(s) 391
BstC8I GCNNGC 2 cut(s) 219, 569
BstDSI CCRYGG 1 cut(s) 190
BstKTI GATC 3 cut(s) 228, 298, 337
BstMAI GTCTC 3 cut(s) 351, 410, 617
BstMBI GATC 3 cut(s) 225, 295, 334
BstMCI CGRYCG 2 cut(s) 121, 250
BstMWI GCNNNNNNNGC 3 cut(s) 214, 352, 464
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 1 cut(s) 571
BstSCI CCNGG 2 cut(s) 120, 238
BstV1I GCAGC 4 cut(s) 220, 223, 226, 230
BstX2I RGATCY 1 cut(s) 225
BstYI RGATCY 1 cut(s) 225
BstZI CGGCCG 1 cut(s) 118
BsuRI GGCC 2 cut(s) 120, 678
BtgI CCRYGG 1 cut(s) 190
BtgZI GCGATG 1 cut(s) 113
BtsI GCAGTG 2 cut(s) 61, 139
BtsIMutI CAGTG 3 cut(s) 61, 139, 202
BveI ACCTGC 1 cut(s) 746
Cac8I GCNNGC 2 cut(s) 219, 569
Cfr10I RCCGGY 1 cut(s) 249
CseI GACGC 2 cut(s) 396, 447
Csp6I GTAC 2 cut(s) 654, 727
CspAI ACCGGT 1 cut(s) 249
CspCI CAANNNNNGTGG 4 cut(s) 172, 207, 677, 712
CviAII CATG 1 cut(s) 568
CviQI GTAC 2 cut(s) 654, 727
DpnI GATC 3 cut(s) 227, 297, 336
DpnII GATC 3 cut(s) 225, 295, 334
DraI TTTAAA 1 cut(s) 637
DraIII CACNNNGTG 1 cut(s) 674
EaeI YGGCCR 2 cut(s) 118, 676
EagI CGGCCG 1 cut(s) 118
Ecl136II GAGCTC 1 cut(s) 328
EclXI CGGCCG 1 cut(s) 118
Eco130I CCWWGG 3 cut(s) 71, 146, 555
Eco24I GRGCYC 1 cut(s) 330
Eco52I CGGCCG 1 cut(s) 118
Eco53kI GAGCTC 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 3 cut(s) 71, 146, 555
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 3 cut(s) 71, 146, 555
FaeI CATG 1 cut(s) 571
FaiI YATR 9 cut(s) 66, 263, 440, 482, 569, 581, 701, 748, 763
FatI CATG 1 cut(s) 567
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 4 cut(s) 209, 212, 215, 244
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 4 cut(s) 209, 212, 215, 244
FspBI CTAG 3 cut(s) 72, 147, 503
GluI GCNGC 4 cut(s) 209, 212, 215, 244
HaeIII GGCC 2 cut(s) 120, 678
HapII CCGG 2 cut(s) 121, 250
HgaI GACGC 2 cut(s) 396, 447
Hin1I GRCGYC 2 cut(s) 407, 458
Hin1II CATG 1 cut(s) 571
HincII GTYRAC 2 cut(s) 411, 462
HindII GTYRAC 2 cut(s) 411, 462
HindIII AAGCTT 1 cut(s) 597
HinfI GANTC 2 cut(s) 140, 359
HpaII CCGG 2 cut(s) 121, 250
Hpy166II GTNNAC 2 cut(s) 411, 462
Hpy188I TCNGA 5 cut(s) 7, 157, 232, 280, 513
Hpy188III TCNNGA 4 cut(s) 15, 338, 371, 614
Hpy8I GTNNAC 2 cut(s) 411, 462
Hpy99I CGWCG 2 cut(s) 54, 201
HpyAV CCTTC 3 cut(s) 95, 245, 611
HpyCH4III ACNGT 4 cut(s) 191, 368, 479, 731
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 4 cut(s) 346, 442, 605, 699
HpyF10VI GCNNNNNNNGC 3 cut(s) 214, 352, 464
HpySE526I ACGT 1 cut(s) 292
Hsp92I GRCGYC 2 cut(s) 407, 458
Hsp92II CATG 1 cut(s) 571
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 3 cut(s) 225, 295, 334
LmnI GCTCC 2 cut(s) 281, 325
Lsp1109I GCAGC 4 cut(s) 220, 223, 226, 230
MaeI CTAG 3 cut(s) 72, 147, 503
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 2 cut(s) 200, 256
MalI GATC 3 cut(s) 227, 297, 336
MboI GATC 3 cut(s) 225, 295, 334
MboII GAAGA 3 cut(s) 392, 405, 676
MfeI CAATTG 2 cut(s) 167, 585
MflI RGATCY 1 cut(s) 225
MhlI GDGCHC 2 cut(s) 278, 330
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 5 cut(s) 112, 167, 506, 537, 585
MluNI TGGCCA 1 cut(s) 678
MlyI GAGTC 2 cut(s) 149, 353
MnlI CCTC 9 cut(s) 38, 52, 130, 310, 316, 424, 586, 640, 753
Mox20I TGGCCA 1 cut(s) 678
MroXI GAANNNNTTC 2 cut(s) 387, 621
MscI TGGCCA 1 cut(s) 678
MseI TTAA 1 cut(s) 636
MslI CAYNNNNRTG 1 cut(s) 132
Msp20I TGGCCA 1 cut(s) 678
MspI CCGG 2 cut(s) 121, 250
MspR9I CCNGG 2 cut(s) 122, 240
MunI CAATTG 2 cut(s) 167, 585
MvaI CCWGG 1 cut(s) 240
MwoI GCNNNNNNNGC 3 cut(s) 214, 352, 464
NciI CCSGG 1 cut(s) 122
NdeII GATC 3 cut(s) 225, 295, 334
NlaIII CATG 1 cut(s) 571
NmuCI GTSAC 2 cut(s) 200, 256
NspI RCATGY 1 cut(s) 571
NspV TTCGAA 1 cut(s) 391
PaeI GCATGC 1 cut(s) 571
PdmI GAANNNNTTC 2 cut(s) 387, 621
PinAI ACCGGT 1 cut(s) 249
PkrI GCNGC 4 cut(s) 210, 213, 216, 245
PleI GAGTC 2 cut(s) 148, 353
PpsI GAGTC 2 cut(s) 148, 353
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 225
RsaI GTAC 2 cut(s) 655, 728
RsaNI GTAC 2 cut(s) 654, 727
RseI CAYNNNNRTG 1 cut(s) 132
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 4 cut(s) 209, 212, 215, 244
Sau3AI GATC 3 cut(s) 225, 295, 334
ScaI AGTACT 1 cut(s) 655
SchI GAGTC 2 cut(s) 149, 353
ScrFI CCNGG 2 cut(s) 122, 240
SduI GDGCHC 2 cut(s) 278, 330
SfuI TTCGAA 1 cut(s) 391
SmiMI CAYNNNNRTG 1 cut(s) 132
SphI GCATGC 1 cut(s) 571
Sse9I AATT 5 cut(s) 112, 167, 506, 537, 585
SsiI CCGC 1 cut(s) 40
SspMI CTAG 3 cut(s) 72, 147, 503
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 2 cut(s) 120, 238
StyI CCWWGG 3 cut(s) 71, 146, 555
TaaI ACNGT 4 cut(s) 191, 368, 479, 731
TaiI ACGT 1 cut(s) 295
TaqI TCGA 3 cut(s) 370, 391, 615
TasI AATT 5 cut(s) 112, 167, 506, 537, 585
TatI WGTACW 2 cut(s) 653, 726
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 3 cut(s) 61, 139, 209
TseFI GTSAC 2 cut(s) 200, 256
TseI GCWGC 4 cut(s) 208, 211, 214, 243
Tsp45I GTSAC 2 cut(s) 200, 256
TspDTI ATGAA 2 cut(s) 278, 614
TspRI CASTG 3 cut(s) 61, 139, 209
XapI RAATTY 1 cut(s) 506
XceI RCATGY 1 cut(s) 571
XmaJI CCTAGG 2 cut(s) 71, 146
XmnI GAANNNNTTC 2 cut(s) 387, 621
XspI CTAG 3 cut(s) 72, 147, 503
ZrmI AGTACT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.