MD17G1091600.v1.1

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006751
Physical Location & Seq
Reverse (-)
7675399 .. 7677740
2342 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1091600.v1.1.491

Sequence Viewer

Length: 1347 bp
ATGATTGGAGTGCTTATAGGAGCGTGTTTCAATCAGTTCAAGAATGTAGGAGATGTTGTAACAATCCCCATTTCCAAATGGGTACTTGAGATCGGAGAATCAGACCCCGATATCTTTTATACCAATCTCAAAGGTAACAGGAACCCGGAATACCTCACTCTAGGTGTGGATAACCTGCGTCTCTTTGACGGACGAACTGCTGTGGAGATATACAGCGATTACATGAAAAGCTTCAGAGAGAACATGTCGGATTTTCTAGAAACCGGACTTATTATAGACATTGAAGTAGGGCTTGGCCCTGCAGGAGAGCTCAGGTACCCCTCTTATCCGGAAAGTCAAGGATGGGCTTTTCCAGGGATTGGAGAATTTCAGTGCTATGACAAGTATCTCCAAGCAGAGTTTAAGGAGGCTGCAACGGTTGCAGGTCATCCTGAATGGGCATTGCCAGTTAACGCCGGAGAATACAATGATACTCCTGAATCCTCAGGGTTCTTCAGATCAAACGGGACGTACGTTACTGAGAAAGGGAAGTTCTTCTTGACTTGGTACTCTAATAAATTACTGATCCATGGTGATCAGATCCTGGATGAAGCCAACAAAGTTTTTGAGGGCTGTAAATTAAAGCTAGCTGCCAAAGTATCTGGAATTCACTGGTGGTATGAAGTTGATAATCATGCCGCGGAGCTTACTGCAGGATACTACAACGTGAAAGATAGAGATGGGTACCGACCTATAGCAAGGATGCTGTCAAGGCATCATGCAATTTTGAATTTCACATGTTTAGAGATGAGGAACTCTGAACAAAGTGCTGACGCCAAAAGTGCGCCTCAGGAACTTGTTCAGCAGGTTTTAAGTGGAGGTTGGAGAGAAAACATAGAAGTTGCCGGAGAAAATGCACTTTCTAGATACGACAGTGTGGCTTATGACCAAATCCTTCTAAATGCTAGACCAAATGGCATCAATAAAGACGGCCAACCAAAATTAAGGATGTACGGGGTGACATTCCTTCGTTTGTCTGATGAACTACTACAAAAGACGAATCTTGATACATTCAAGATATTTGTAAAAAAGATGCATGCTGATCAGGATTACTGTTCAGACCCAGAGAAGTACAATCACCACCTAGGTCCTCTGAAACAGTCGAAACCAGAGATTTCGATTGAAGATCTTCTGGAAGCAACCAAAGCATTGGAGCCATTCCCATGGGATAAAGAAACAGATATGAGTGTCAGTGGTGGAGCTGGTGGACTTGGTGGGCTACTCTCTAATTTGATAAGAAAAATCTTCTCTCTAGTTAAGCGATACAACTGCCAAGCTGGGAGTGAAGCAACCAATAAATTGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

449

Amino Acids

50.33

Weight (kDa)

5.24

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 14 - 340 2.2e-97 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 183, 413, 835
Acc65I GGTACC 2 cut(s) 315, 723
AccB1I GGYRCC 2 cut(s) 315, 723
AccB7I CCANNNNNTGG 1 cut(s) 359
AccII CGCG 1 cut(s) 680
AccIII TCCGGA 1 cut(s) 328
AciI CCGC 2 cut(s) 678, 680
AclWI GGATC 2 cut(s) 559, 574
AcoI YGGCCR 1 cut(s) 970
AcsI RAATTY 3 cut(s) 365, 645, 769
AcuI CTGAAG 2 cut(s) 217, 478
AcyI GRCGYC 1 cut(s) 813
AfaI GTAC 7 cut(s) 84, 317, 512, 548, 725, 992, 1112
AfiI CCNNNNNNNGG 1 cut(s) 359
AflIII ACRYGT 2 cut(s) 243, 776
AgsI TTSAA 6 cut(s) 31, 40, 284, 769, 1054, 1163
AjnI CCWGG 2 cut(s) 352, 582
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AluBI AGCT 7 cut(s) 231, 310, 625, 629, 685, 1241, 1316
AluI AGCT 7 cut(s) 231, 310, 625, 629, 685, 1241, 1316
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 1 cut(s) 185
AlwI GGATC 2 cut(s) 559, 574
AlwNI CAGNNNCTG 1 cut(s) 583
Aor13HI TCCGGA 1 cut(s) 328
AoxI GGCC 2 cut(s) 295, 970
ApeKI GCWGC 2 cut(s) 410, 629
ApoI RAATTY 3 cut(s) 365, 645, 769
Asp700I GAANNNNTTC 4 cut(s) 230, 533, 837, 1167
Asp718I GGTACC 2 cut(s) 315, 723
AspA2I CCTAGG 1 cut(s) 1123
AspLEI GCGC 1 cut(s) 826
AspS9I GGNCC 2 cut(s) 296, 1127
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 3 cut(s) 584, 1009, 1109
AsuNHI GCTAGC 1 cut(s) 625
AvaII GGWCC 1 cut(s) 1127
AvrII CCTAGG 1 cut(s) 1123
AxyI CCTNAGG 2 cut(s) 484, 828
BaeI ACNNNNGTAYC 2 cut(s) 898, 931
BanI GGYRCC 2 cut(s) 315, 723
BanII GRGCYC 1 cut(s) 312
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 2 cut(s) 397, 616
BccI CCATC 2 cut(s) 336, 713
BceAI ACGGC 1 cut(s) 985
BcgI CGANNNNNNTGC 2 cut(s) 1290, 1324
BciT130I CCWGG 2 cut(s) 354, 584
BciVI GTATCC 1 cut(s) 689
BclI TGATCA 2 cut(s) 574, 1081
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 185
BfaI CTAG 7 cut(s) 161, 257, 626, 903, 945, 1124, 1292
BfmI CTRYAG 3 cut(s) 300, 690, 732
BfuAI ACCTGC 3 cut(s) 183, 413, 835
BfuI GTATCC 1 cut(s) 689
BglII AGATCT 1 cut(s) 1165
BisI GCNGC 3 cut(s) 411, 630, 678
BlnI CCTAGG 1 cut(s) 1123
BlsI GCNGC 3 cut(s) 412, 631, 679
Bme1390I CCNGG 3 cut(s) 146, 354, 584
Bme18I GGWCC 1 cut(s) 1127
BmgT120I GGNCC 2 cut(s) 296, 1127
BmiI GGNNCC 4 cut(s) 143, 317, 725, 1194
BmrFI CCNGG 3 cut(s) 146, 354, 584
BmsI GCATC 4 cut(s) 732, 763, 966, 1062
BmtI GCTAGC 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 146
BsaHI GRCGYC 1 cut(s) 813
BsaJI CCNNGG 5 cut(s) 353, 568, 678, 1123, 1202
BsaWI WCCGGW 2 cut(s) 263, 328
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse1I ACTGG 2 cut(s) 446, 656
Bse21I CCTNAGG 2 cut(s) 484, 828
Bse3DI GCAATG 1 cut(s) 440
BseAI TCCGGA 1 cut(s) 328
BseBI CCWGG 2 cut(s) 354, 584
BseDI CCNNGG 5 cut(s) 353, 568, 678, 1123, 1202
BseGI GGATG 5 cut(s) 347, 427, 592, 747, 993
BseLI CCNNNNNNNGG 1 cut(s) 359
BseMI GCAATG 1 cut(s) 440
BseMII CTCAG 4 cut(s) 325, 498, 510, 842
BseNI ACTGG 2 cut(s) 446, 656
BseXI GCAGC 2 cut(s) 397, 616
BseYI CCCAGC 1 cut(s) 1316
Bsh1236I CGCG 1 cut(s) 680
BshFI GGCC 2 cut(s) 297, 972
BshNI GGYRCC 2 cut(s) 315, 723
BsiHKAI GWGCWC 1 cut(s) 312
BsiSI CCGG 5 cut(s) 146, 264, 329, 456, 885
BsiWI CGTACG 1 cut(s) 510
BslFI GGGAC 1 cut(s) 520
BslI CCNNNNNNNGG 1 cut(s) 359
BsmAI GTCTC 1 cut(s) 185
BsmBI CGTCTC 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 520
BsnI GGCC 2 cut(s) 297, 972
Bsp1286I GDGCHC 1 cut(s) 312
Bsp13I TCCGGA 1 cut(s) 328
Bsp143I GATC 7 cut(s) 90, 497, 564, 574, 579, 1081, 1165
Bsp19I CCATGG 2 cut(s) 568, 1202
BspACI CCGC 2 cut(s) 678, 680
BspANI GGCC 2 cut(s) 297, 972
BspCNI CTCAG 4 cut(s) 324, 497, 511, 841
BspEI TCCGGA 1 cut(s) 328
BspFNI CGCG 1 cut(s) 680
BspLI GGNNCC 4 cut(s) 143, 317, 725, 1194
BspMAI CTGCAG 2 cut(s) 304, 694
BspMI ACCTGC 3 cut(s) 183, 413, 835
BspOI GCTAGC 1 cut(s) 629
BspPI GGATC 2 cut(s) 559, 574
BspT107I GGYRCC 2 cut(s) 315, 723
BsrDI GCAATG 1 cut(s) 440
BsrI ACTGG 2 cut(s) 446, 656
BssECI CCNNGG 5 cut(s) 353, 568, 678, 1123, 1202
BssMI GATC 7 cut(s) 90, 497, 564, 574, 579, 1081, 1165
BssNI GRCGYC 1 cut(s) 813
BssT1I CCWWGG 3 cut(s) 568, 1123, 1202
Bst2UI CCWGG 2 cut(s) 354, 584
Bst4CI ACNGT 4 cut(s) 418, 914, 1094, 1140
BstACI GRCGYC 1 cut(s) 813
BstAPI GCANNNNNTGC 1 cut(s) 419
BstC8I GCNNGC 2 cut(s) 627, 1077
BstDEI CTNAG 4 cut(s) 311, 484, 519, 828
BstDSI CCRYGG 3 cut(s) 568, 678, 1202
BstF5I GGATG 5 cut(s) 347, 427, 592, 747, 993
BstFNI CGCG 1 cut(s) 680
BstHHI GCGC 1 cut(s) 826
BstKTI GATC 7 cut(s) 93, 500, 567, 577, 582, 1084, 1168
BstMAI GTCTC 1 cut(s) 185
BstMBI GATC 7 cut(s) 90, 497, 564, 574, 579, 1081, 1165
BstMWI GCNNNNNNNGC 4 cut(s) 419, 751, 821, 1184
BstNI CCWGG 2 cut(s) 354, 584
BstNSI RCATGY 3 cut(s) 247, 780, 1079
BstSCI CCNGG 3 cut(s) 144, 352, 582
BstSFI CTRYAG 3 cut(s) 300, 690, 732
BstUI CGCG 1 cut(s) 680
BstV1I GCAGC 2 cut(s) 397, 616
BstX2I RGATCY 2 cut(s) 579, 1165
BstXI CCANNNNNNTGG 3 cut(s) 1189, 1203, 1339
BstYI RGATCY 2 cut(s) 579, 1165
Bsu36I CCTNAGG 2 cut(s) 484, 828
BsuI GTATCC 1 cut(s) 689
BsuRI GGCC 2 cut(s) 297, 972
BtgI CCRYGG 3 cut(s) 568, 678, 1202
BtsCI GGATG 5 cut(s) 347, 427, 592, 747, 993
BtsIMutI CAGTG 4 cut(s) 377, 649, 919, 1237
BveI ACCTGC 3 cut(s) 183, 413, 835
Cac8I GCNNGC 2 cut(s) 627, 1077
CaiI CAGNNNCTG 1 cut(s) 583
CfoI GCGC 1 cut(s) 826
Cfr13I GGNCC 2 cut(s) 296, 1127
Cfr42I CCGCGG 1 cut(s) 681
CseI GACGC 2 cut(s) 167, 821
Csp6I GTAC 7 cut(s) 83, 316, 511, 547, 724, 991, 1111
CviAII CATG 8 cut(s) 223, 244, 569, 674, 758, 777, 1076, 1203
CviQI GTAC 7 cut(s) 83, 316, 511, 547, 724, 991, 1111
DdeI CTNAG 4 cut(s) 311, 484, 519, 828
DpnI GATC 7 cut(s) 92, 499, 566, 576, 581, 1083, 1167
DpnII GATC 7 cut(s) 90, 497, 564, 574, 579, 1081, 1165
EaeI YGGCCR 1 cut(s) 970
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 3 cut(s) 568, 1123, 1202
Eco24I GRGCYC 1 cut(s) 312
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 1127
Eco53kI GAGCTC 1 cut(s) 310
Eco57I CTGAAG 2 cut(s) 217, 478
Eco81I CCTNAGG 2 cut(s) 484, 828
EcoICRI GAGCTC 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 1127
EcoRI GAATTC 1 cut(s) 645
EcoRII CCWGG 2 cut(s) 352, 582
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 3 cut(s) 568, 1123, 1202
EcoT22I ATGCAT 1 cut(s) 1077
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 3 cut(s) 568, 1123, 1202
Esp3I CGTCTC 1 cut(s) 185
FaeI CATG 8 cut(s) 226, 247, 572, 677, 761, 780, 1079, 1206
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 521, 553
FaqI GGGAC 1 cut(s) 520
FatI CATG 8 cut(s) 222, 243, 568, 673, 757, 776, 1075, 1202
FbaI TGATCA 2 cut(s) 574, 1081
Fnu4HI GCNGC 3 cut(s) 411, 630, 678
FokI GGATG 5 cut(s) 354, 414, 599, 754, 1000
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 3 cut(s) 411, 630, 678
FspBI CTAG 7 cut(s) 161, 257, 626, 903, 945, 1124, 1292
GlaI GCGC 1 cut(s) 825
GluI GCNGC 3 cut(s) 411, 630, 678
GsaI CCCAGC 1 cut(s) 1320
HaeIII GGCC 2 cut(s) 297, 972
HapII CCGG 5 cut(s) 146, 264, 329, 456, 885
HgaI GACGC 2 cut(s) 167, 821
HhaI GCGC 1 cut(s) 826
Hin1I GRCGYC 1 cut(s) 813
Hin1II CATG 8 cut(s) 226, 247, 572, 677, 761, 780, 1079, 1206
Hin6I GCGC 1 cut(s) 824
HinP1I GCGC 1 cut(s) 824
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HindIII AAGCTT 1 cut(s) 229
HinfI GANTC 3 cut(s) 98, 479, 1039
HpaI GTTAAC 1 cut(s) 451
HpaII CCGG 5 cut(s) 146, 264, 329, 456, 885
HphI GGTGA 3 cut(s) 584, 1009, 1109
Hpy166II GTNNAC 2 cut(s) 451, 1247
Hpy8I GTNNAC 2 cut(s) 451, 1247
HpyAV CCTTC 2 cut(s) 944, 1016
HpyCH4III ACNGT 4 cut(s) 418, 914, 1094, 1140
HpyCH4IV ACGT 3 cut(s) 509, 513, 705
HpyCH4V TGCA 7 cut(s) 302, 413, 422, 692, 761, 896, 1075
HpyF10VI GCNNNNNNNGC 4 cut(s) 419, 751, 821, 1184
HpyF3I CTNAG 4 cut(s) 311, 484, 519, 828
HpySE526I ACGT 3 cut(s) 509, 513, 705
Hsp92I GRCGYC 1 cut(s) 813
Hsp92II CATG 8 cut(s) 226, 247, 572, 677, 761, 780, 1079, 1206
HspAI GCGC 1 cut(s) 824
Kpn2I TCCGGA 1 cut(s) 328
KpnI GGTACC 2 cut(s) 319, 727
Ksp22I TGATCA 2 cut(s) 574, 1081
KspAI GTTAAC 1 cut(s) 451
KspI CCGCGG 1 cut(s) 681
Kzo9I GATC 7 cut(s) 90, 497, 564, 574, 579, 1081, 1165
LmnI GCTCC 4 cut(s) 20, 682, 1192, 1238
Lsp1109I GCAGC 2 cut(s) 397, 616
LweI GCATC 4 cut(s) 732, 763, 966, 1062
MaeI CTAG 7 cut(s) 161, 257, 626, 903, 945, 1124, 1292
MaeII ACGT 3 cut(s) 509, 513, 705
MaeIII GTNAC 4 cut(s) 58, 134, 514, 997
MalI GATC 7 cut(s) 92, 499, 566, 576, 581, 1083, 1167
MboI GATC 7 cut(s) 90, 497, 564, 574, 579, 1081, 1165
MboII GAAGA 5 cut(s) 484, 526, 1160, 1175, 1276
MflI RGATCY 2 cut(s) 579, 1165
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 9 cut(s) 365, 557, 617, 645, 762, 769, 980, 1267, 1337
MmeI TCCRAC 2 cut(s) 228, 842
MnlI CCTC 9 cut(s) 164, 331, 400, 493, 601, 783, 837, 851, 1140
Mph1103I ATGCAT 1 cut(s) 1077
MroI TCCGGA 1 cut(s) 328
MroXI GAANNNNTTC 4 cut(s) 230, 533, 837, 1167
MseI TTAA 6 cut(s) 402, 450, 620, 851, 983, 1296
MslI CAYNNNNRTG 1 cut(s) 1201
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 5 cut(s) 146, 264, 329, 456, 885
MspR9I CCNGG 3 cut(s) 146, 354, 584
MvaI CCWGG 2 cut(s) 354, 584
MvnI CGCG 1 cut(s) 680
MwoI GCNNNNNNNGC 4 cut(s) 419, 751, 821, 1184
NciI CCSGG 1 cut(s) 146
NcoI CCATGG 2 cut(s) 568, 1202
NdeII GATC 7 cut(s) 90, 497, 564, 574, 579, 1081, 1165
NheI GCTAGC 1 cut(s) 625
NlaIII CATG 8 cut(s) 226, 247, 572, 677, 761, 780, 1079, 1206
NlaIV GGNNCC 4 cut(s) 143, 317, 725, 1194
NmuCI GTSAC 1 cut(s) 997
NsiI ATGCAT 1 cut(s) 1077
NspI RCATGY 3 cut(s) 247, 780, 1079
PaeI GCATGC 1 cut(s) 1079
PciI ACATGT 2 cut(s) 243, 776
PcsI WCGNNNNNNNCGW 1 cut(s) 510
PdmI GAANNNNTTC 4 cut(s) 230, 533, 837, 1167
PfeI GAWTC 3 cut(s) 98, 479, 1039
Pfl23II CGTACG 1 cut(s) 510
PflMI CCANNNNNTGG 1 cut(s) 359
PfoI TCCNGGA 1 cut(s) 582
PkrI GCNGC 3 cut(s) 412, 631, 679
PpuMI RGGWCCY 1 cut(s) 1127
PscI ACATGT 2 cut(s) 243, 776
Psp124BI GAGCTC 1 cut(s) 312
Psp5II RGGWCCY 1 cut(s) 1127
Psp6I CCWGG 2 cut(s) 352, 582
PspFI CCCAGC 1 cut(s) 1316
PspGI CCWGG 2 cut(s) 352, 582
PspLI CGTACG 1 cut(s) 510
PspN4I GGNNCC 4 cut(s) 143, 317, 725, 1194
PspPI GGNCC 2 cut(s) 296, 1127
PspPPI RGGWCCY 1 cut(s) 1127
PsrI GAACNNNNNNTAC 2 cut(s) 134, 166
PstI CTGCAG 2 cut(s) 304, 694
PstNI CAGNNNCTG 1 cut(s) 583
PsuI RGATCY 2 cut(s) 579, 1165
RsaI GTAC 7 cut(s) 84, 317, 512, 548, 725, 992, 1112
RsaNI GTAC 7 cut(s) 83, 316, 511, 547, 724, 991, 1111
RseI CAYNNNNRTG 1 cut(s) 1201
SacI GAGCTC 1 cut(s) 312
SacII CCGCGG 1 cut(s) 681
SaqAI TTAA 6 cut(s) 402, 450, 620, 851, 983, 1296
SatI GCNGC 3 cut(s) 411, 630, 678
Sau3AI GATC 7 cut(s) 90, 497, 564, 574, 579, 1081, 1165
Sau96I GGNCC 2 cut(s) 296, 1127
SbfI CCTGCAGG 1 cut(s) 304
ScrFI CCNGG 3 cut(s) 146, 354, 584
SdaI CCTGCAGG 1 cut(s) 304
SduI GDGCHC 1 cut(s) 312
SfaNI GCATC 4 cut(s) 732, 763, 966, 1062
SfcI CTRYAG 3 cut(s) 300, 690, 732
Sfr303I CCGCGG 1 cut(s) 681
SgrBI CCGCGG 1 cut(s) 681
SinI GGWCC 1 cut(s) 1127
SmiMI CAYNNNNRTG 1 cut(s) 1201
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SphI GCATGC 1 cut(s) 1079
Sse8387I CCTGCAGG 1 cut(s) 304
Sse9I AATT 9 cut(s) 365, 557, 617, 645, 762, 769, 980, 1267, 1337
SsiI CCGC 2 cut(s) 678, 680
SspMI CTAG 7 cut(s) 161, 257, 626, 903, 945, 1124, 1292
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 3 cut(s) 144, 352, 582
StyI CCWWGG 3 cut(s) 568, 1123, 1202
TaaI ACNGT 4 cut(s) 418, 914, 1094, 1140
TaiI ACGT 3 cut(s) 512, 516, 708
TaqI TCGA 2 cut(s) 1142, 1157
TasI AATT 9 cut(s) 365, 557, 617, 645, 762, 769, 980, 1267, 1337
TatI WGTACW 1 cut(s) 1110
TauI GCSGC 1 cut(s) 680
TfiI GAWTC 3 cut(s) 98, 479, 1039
Tru1I TTAA 6 cut(s) 402, 450, 620, 851, 983, 1296
Tru9I TTAA 6 cut(s) 402, 450, 620, 851, 983, 1296
TscAI CASTG 4 cut(s) 377, 656, 919, 1237
TseFI GTSAC 1 cut(s) 997
TseI GCWGC 2 cut(s) 410, 629
Tsp45I GTSAC 1 cut(s) 997
TspDTI ATGAA 4 cut(s) 239, 603, 675, 1035
TspGWI ACGGA 1 cut(s) 204
TspRI CASTG 4 cut(s) 377, 656, 919, 1237
Van91I CCANNNNNTGG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 1127
XapI RAATTY 3 cut(s) 365, 645, 769
XbaI TCTAGA 2 cut(s) 256, 902
XceI RCATGY 3 cut(s) 247, 780, 1079
XmaJI CCTAGG 1 cut(s) 1123
XmnI GAANNNNTTC 4 cut(s) 230, 533, 837, 1167
XspI CTAG 7 cut(s) 161, 257, 626, 903, 945, 1124, 1292
Zsp2I ATGCAT 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.