RLG00000021210

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006751
Physical Location & Seq
Forward (+)
72950467 .. 72953451
2985 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021210

Sequence Viewer

Length: 1749 bp
ATGGCAAGCACAACTCTAGGTACTCTGGGAGCAAAAACTAAGCTTCATCATGGGAGGTACTTGTGCAGAGATGTTCCATTTCAAAATCTACTTGGAAGTGCAGAAGCAACAAACAAAGCATGGAGGCAAAGATCAAATAAGAGTGCTTCCACGTTCATTATAAGTCCTCGAGCTGTGGCATCTGAAATTTCCACCACACAAAGAGAGGATGCACCTACACTCACATTCAACGAGAAGATGCTTGCAAATTATGTGCCAATCTTTGTGATGCTCCCTCTGGGAGTTGTTACGATTGATAATGTTTTAGAAGACAAAGACACACTCGTGAAGCAGCTGAAGGAACTGAAGAAAGCAGGTGTTGATGGAGTAATGGTAGATGTGTGGTGGGGGATCATAGAATCAAAAGGGCCAAAACAATATGACTGGAGTGCTTATAGGAGCATGTTTCAAGCAGTTCAAGAATGTGGACTGAAATTACAAGCTATAATGTCATTTCACCAATGTGGTGGGAATATAGGAGATATTGTAACCATTCCCATCCCCCAATGGGTACTTGATATCGGAGAATCGAACCCTGACATCTTTTATACCAATCTCAAAGGTAACAGGAACCGGGAGTACCTCACTCTAGGTGTGGATAACTTGCCTCTCTTTAATGGAAGAACTGCTGTTGAGATATATAGTGACTACTTGAAGAGCTACAGAGAGAACATGTCCGATTTTCTAGAAAGTGGACTAATTATAGACATTGAAGTGGGACTTGGCCCTGCAGGAGAGCTCAGGTACCCCTCTTATCCGGAAACTCAAGGATGGGCTTTTCCAGGAATTGGAGAATTTCAGTATCTCAAAGCAGAGTTCAAAGAGGCTGCAGCAATTGCTGGCCATCCTGAATGGGATTTGCCAGATAATGCAGGAGAATACAATGATACTCCTGAATCTACAGAGTTCTTTAAATCAAATGGGACATACCTTACTGAGAAAGGGAAGTTCTTTTTGACTTGGTACTCTAACAAATTACTGAGCCATGGTGATCAGATCCTGGATGAAGCCAACAAAGTTTTCCTGGGCTGTAAATTAAAGCTAGCAGCAAAAGTCTCTGGAATCCACTGGTGGTATGAAACTGATAATCATGCTGCGGAGCTTACCGCAGGATACTATAACCTGAAGGATAGAGATGGGTACCGACCTATAGCAAGGATGCTGTCAAGGCACCATGCCATTTTGAATTTCACATGTTTAGAGATGAAGAACTCTGAACAAAGTGCCGATGCCAAAAGTGCGCCTCAAGAACTCGTTCAGCAGGTTTTGAGTGGAGGTTGGAGAGAGAACATAGAAGTTGCAGGGGAAAATGCACTTGCAAGGTATGATAGTGCAGCTTATGACCAAATCCTTTTAAATGCTAGACCAAATGGCATCAACAAAGATGGCCCACCAAAATTAAGGATGTATGGGGTAACTTACCTTCGTCTGGTGGATGAACTCCTACAAGAAACGAATTTCATTATATTCAAGACATTTGTAAAGAAGATGCATGCTGATCAGGATTACTGTCAAGACTCAGAGAAGTACAACCACCGCCTAGGTCCCTTGGAACGTTCGAAACCAAAGTTCTCAACTGAAGATCTTCTGGATGCAACAAAACCAGTGGAGCCATTCCCGTGGGATAAAGAAACAGATATGAGTGTCACTGGTGGTGGTGGTGGTGTACTTTCTTATTTGATAAGCAAAATCTTTTCTCTATTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

583

Amino Acids

65.51

Weight (kDa)

5.55

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 88 - 494 1.4e-131 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 161
AarI CACCTGC 1 cut(s) 344
Acc36I ACCTGC 2 cut(s) 344, 1291
Acc65I GGTACC 2 cut(s) 783, 1179
AccB1I GGYRCC 3 cut(s) 783, 1179, 1209
AccB7I CCANNNNNTGG 1 cut(s) 827
AccIII TCCGGA 1 cut(s) 796
AciI CCGC 3 cut(s) 1136, 1146, 1576
AclI AACGTT 1 cut(s) 1594
AclWI GGATC 2 cut(s) 398, 1030
AcoI YGGCCR 1 cut(s) 880
AcsI RAATTY 4 cut(s) 186, 833, 1225, 1495
AcuI CTGAAG 4 cut(s) 356, 365, 1184, 1638
AfaI GTAC 9 cut(s) 22, 59, 552, 620, 785, 1004, 1181, 1568, 1707
AfiI CCNNNNNNNGG 3 cut(s) 547, 827, 1468
AflIII ACRYGT 2 cut(s) 711, 1232
AgsI TTSAA 9 cut(s) 83, 229, 449, 458, 694, 752, 859, 1225, 1510
AjnI CCWGG 3 cut(s) 820, 1038, 1062
AjuI GAANNNNNNNTTGG 4 cut(s) 744, 776, 1043, 1075
AleI CACNNNNGTG 2 cut(s) 323, 501
AluBI AGCT 9 cut(s) 43, 173, 334, 482, 699, 778, 1081, 1141, 1376
AluI AGCT 9 cut(s) 43, 173, 334, 482, 699, 778, 1081, 1141, 1376
Alw21I GWGCWC 1 cut(s) 780
Alw26I GTCTC 1 cut(s) 1099
AlwI GGATC 2 cut(s) 398, 1030
AlwNI CAGNNNCTG 1 cut(s) 1039
Ama87I CYCGRG 1 cut(s) 168
Aor13HI TCCGGA 1 cut(s) 796
AoxI GGCC 4 cut(s) 407, 763, 880, 1426
ApeKI GCWGC 6 cut(s) 331, 866, 869, 1085, 1133, 1373
ApoI RAATTY 4 cut(s) 186, 833, 1225, 1495
Asp700I GAANNNNTTC 2 cut(s) 1293, 1623
Asp718I GGTACC 2 cut(s) 783, 1179
AspA2I CCTAGG 1 cut(s) 1579
AspLEI GCGC 1 cut(s) 1282
AspS9I GGNCC 4 cut(s) 407, 764, 1427, 1583
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 2 cut(s) 488, 1040
AsuII TTCGAA 1 cut(s) 1598
AsuNHI GCTAGC 1 cut(s) 1081
AvaI CYCGRG 1 cut(s) 168
AvaII GGWCC 1 cut(s) 1583
AvrII CCTAGG 1 cut(s) 1579
BalI TGGCCA 1 cut(s) 882
BanI GGYRCC 3 cut(s) 783, 1179, 1209
BanII GRGCYC 1 cut(s) 780
BarI GAAGNNNNNNTAC 2 cut(s) 1446, 1478
BauI CACGAG 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 780
BbvI GCAGC 6 cut(s) 343, 853, 881, 1097, 1120, 1385
BccI CCATC 6 cut(s) 356, 545, 804, 891, 1169, 1418
BciT130I CCWGG 3 cut(s) 822, 1040, 1064
BciVI GTATCC 1 cut(s) 1145
BclI TGATCA 2 cut(s) 1030, 1537
BcnI CCSGG 1 cut(s) 614
BcoDI GTCTC 1 cut(s) 1099
BfaI CTAG 6 cut(s) 17, 629, 725, 1082, 1401, 1580
BfmI CTRYAG 5 cut(s) 700, 768, 867, 939, 1188
BfuAI ACCTGC 2 cut(s) 344, 1291
BfuI GTATCC 1 cut(s) 1145
BglII AGATCT 1 cut(s) 1621
BisI GCNGC 6 cut(s) 332, 867, 870, 1086, 1134, 1374
BlnI CCTAGG 1 cut(s) 1579
BlsI GCNGC 6 cut(s) 333, 868, 871, 1087, 1135, 1375
Bme1390I CCNGG 4 cut(s) 614, 822, 1040, 1064
Bme18I GGWCC 1 cut(s) 1583
BmeT110I CYCGRG 1 cut(s) 168
BmgT120I GGNCC 4 cut(s) 407, 764, 1427, 1583
BmiI GGNNCC 6 cut(s) 611, 785, 1181, 1211, 1585, 1650
BmrFI CCNGG 4 cut(s) 614, 822, 1040, 1064
BmsI GCATC 9 cut(s) 188, 199, 228, 258, 1188, 1258, 1422, 1518, 1621
BmtI GCTAGC 1 cut(s) 1085
BpiI GAAGAC 1 cut(s) 315
BpmI CTGGAG 1 cut(s) 445
Bpu10I CCTNAGC 1 cut(s) 779
Bpu14I TTCGAA 1 cut(s) 1598
BpuEI CTTGAG 2 cut(s) 789, 1269
BpuMI CCSGG 1 cut(s) 614
BsaJI CCNNGG 5 cut(s) 1024, 1063, 1579, 1587, 1658
BsaWI WCCGGW 1 cut(s) 796
BsaXI ACNNNNNCTCC 4 cut(s) 357, 387, 510, 540
Bsc4I CCNNNNNNNGG 3 cut(s) 547, 827, 1468
Bse1I ACTGG 4 cut(s) 428, 1112, 1643, 1693
BseAI TCCGGA 1 cut(s) 796
BseBI CCWGG 3 cut(s) 822, 1040, 1064
BseDI CCNNGG 5 cut(s) 1024, 1063, 1579, 1587, 1658
BseGI GGATG 9 cut(s) 214, 537, 815, 883, 1048, 1203, 1449, 1480, 1636
BseLI CCNNNNNNNGG 3 cut(s) 547, 827, 1468
BseMII CTCAG 4 cut(s) 793, 966, 1010, 1572
BseNI ACTGG 4 cut(s) 428, 1112, 1643, 1693
BseXI GCAGC 6 cut(s) 343, 853, 881, 1097, 1120, 1385
BsgI GTGCAG 3 cut(s) 85, 120, 1392
BshFI GGCC 4 cut(s) 409, 765, 882, 1428
BshNI GGYRCC 3 cut(s) 783, 1179, 1209
BsiHKAI GWGCWC 1 cut(s) 780
BsiHKCI CYCGRG 1 cut(s) 168
BsiSI CCGG 2 cut(s) 613, 797
BslFI GGGAC 3 cut(s) 771, 976, 1569
BslI CCNNNNNNNGG 3 cut(s) 547, 827, 1468
BsmAI GTCTC 1 cut(s) 1099
BsmFI GGGAC 3 cut(s) 771, 976, 1569
BsnI GGCC 4 cut(s) 409, 765, 882, 1428
BsoBI CYCGRG 1 cut(s) 168
Bsp119I TTCGAA 1 cut(s) 1598
Bsp1286I GDGCHC 1 cut(s) 780
Bsp13I TCCGGA 1 cut(s) 796
Bsp143I GATC 6 cut(s) 131, 390, 1030, 1035, 1537, 1621
Bsp19I CCATGG 1 cut(s) 1024
BspACI CCGC 3 cut(s) 1136, 1146, 1576
BspANI GGCC 4 cut(s) 409, 765, 882, 1428
BspCNI CTCAG 4 cut(s) 792, 967, 1011, 1571
BspEI TCCGGA 1 cut(s) 796
BspLI GGNNCC 6 cut(s) 611, 785, 1181, 1211, 1585, 1650
BspMAI CTGCAG 2 cut(s) 772, 871
BspMI ACCTGC 2 cut(s) 344, 1291
BspOI GCTAGC 1 cut(s) 1085
BspPI GGATC 2 cut(s) 398, 1030
BspQI GCTCTTC 1 cut(s) 689
BspT104I TTCGAA 1 cut(s) 1598
BspT107I GGYRCC 3 cut(s) 783, 1179, 1209
BsrI ACTGG 4 cut(s) 428, 1112, 1643, 1693
BssECI CCNNGG 5 cut(s) 1024, 1063, 1579, 1587, 1658
BssMI GATC 6 cut(s) 131, 390, 1030, 1035, 1537, 1621
BssSI CACGAG 1 cut(s) 323
BssT1I CCWWGG 3 cut(s) 1024, 1579, 1587
Bst2BI CACGAG 1 cut(s) 323
Bst2UI CCWGG 3 cut(s) 822, 1040, 1064
Bst4CI ACNGT 1 cut(s) 1550
Bst6I CTCTTC 1 cut(s) 689
BstAPI GCANNNNNTGC 1 cut(s) 875
BstBI TTCGAA 1 cut(s) 1598
BstC8I GCNNGC 5 cut(s) 7, 243, 880, 1083, 1533
BstDEI CTNAG 5 cut(s) 39, 779, 975, 1019, 1558
BstDSI CCRYGG 2 cut(s) 1024, 1658
BstF5I GGATG 9 cut(s) 214, 537, 815, 883, 1048, 1203, 1449, 1480, 1636
BstHHI GCGC 1 cut(s) 1282
BstKTI GATC 6 cut(s) 134, 393, 1033, 1038, 1540, 1624
BstMAI GTCTC 1 cut(s) 1099
BstMBI GATC 6 cut(s) 131, 390, 1030, 1035, 1537, 1621
BstMWI GCNNNNNNNGC 3 cut(s) 875, 1207, 1277
BstNI CCWGG 3 cut(s) 822, 1040, 1064
BstNSI RCATGY 4 cut(s) 445, 715, 1236, 1535
BstSCI CCNGG 4 cut(s) 612, 820, 1038, 1062
BstSFI CTRYAG 5 cut(s) 700, 768, 867, 939, 1188
BstV1I GCAGC 6 cut(s) 343, 853, 881, 1097, 1120, 1385
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 2 cut(s) 1035, 1621
BstXI CCANNNNNNTGG 2 cut(s) 506, 1659
BstYI RGATCY 2 cut(s) 1035, 1621
BsuI GTATCC 1 cut(s) 1145
BsuRI GGCC 4 cut(s) 409, 765, 882, 1428
BtgI CCRYGG 2 cut(s) 1024, 1658
BtsCI GGATG 9 cut(s) 214, 537, 815, 883, 1048, 1203, 1449, 1480, 1636
BtsIMutI CAGTG 3 cut(s) 1105, 1650, 1686
BveI ACCTGC 2 cut(s) 344, 1291
Cac8I GCNNGC 5 cut(s) 7, 243, 880, 1083, 1533
CaiI CAGNNNCTG 1 cut(s) 1039
CfoI GCGC 1 cut(s) 1282
Cfr13I GGNCC 4 cut(s) 407, 764, 1427, 1583
Csp6I GTAC 9 cut(s) 21, 58, 551, 619, 784, 1003, 1180, 1567, 1706
CspCI CAANNNNNGTGG 2 cut(s) 1626, 1661
CviAII CATG 9 cut(s) 50, 120, 442, 712, 1025, 1130, 1214, 1233, 1532
CviQI GTAC 9 cut(s) 21, 58, 551, 619, 784, 1003, 1180, 1567, 1706
DdeI CTNAG 5 cut(s) 39, 779, 975, 1019, 1558
DpnI GATC 6 cut(s) 133, 392, 1032, 1037, 1539, 1623
DpnII GATC 6 cut(s) 131, 390, 1030, 1035, 1537, 1621
DraI TTTAAA 2 cut(s) 952, 1395
EaeI YGGCCR 1 cut(s) 880
Eam1104I CTCTTC 1 cut(s) 689
EarI CTCTTC 1 cut(s) 689
Ecl136II GAGCTC 1 cut(s) 778
Eco130I CCWWGG 3 cut(s) 1024, 1579, 1587
Eco24I GRGCYC 1 cut(s) 780
Eco32I GATATC 1 cut(s) 559
Eco47I GGWCC 1 cut(s) 1583
Eco53kI GAGCTC 1 cut(s) 778
Eco57I CTGAAG 4 cut(s) 356, 365, 1184, 1638
Eco88I CYCGRG 1 cut(s) 168
EcoICRI GAGCTC 1 cut(s) 778
EcoO109I RGGNCCY 1 cut(s) 1583
EcoRII CCWGG 3 cut(s) 820, 1038, 1062
EcoRV GATATC 1 cut(s) 559
EcoT14I CCWWGG 3 cut(s) 1024, 1579, 1587
EcoT22I ATGCAT 1 cut(s) 1533
EcoT38I GRGCYC 1 cut(s) 780
ErhI CCWWGG 3 cut(s) 1024, 1579, 1587
FaeI CATG 9 cut(s) 53, 123, 445, 715, 1028, 1133, 1217, 1236, 1535
FalI AAGNNNNNCTT 2 cut(s) 744, 776
FaqI GGGAC 3 cut(s) 771, 976, 1569
FatI CATG 9 cut(s) 49, 119, 441, 711, 1024, 1129, 1213, 1232, 1531
FbaI TGATCA 2 cut(s) 1030, 1537
Fnu4HI GCNGC 6 cut(s) 332, 867, 870, 1086, 1134, 1374
FokI GGATG 9 cut(s) 221, 524, 822, 870, 1055, 1210, 1456, 1487, 1643
FriOI GRGCYC 1 cut(s) 780
Fsp4HI GCNGC 6 cut(s) 332, 867, 870, 1086, 1134, 1374
FspBI CTAG 6 cut(s) 17, 629, 725, 1082, 1401, 1580
GlaI GCGC 1 cut(s) 1281
GluI GCNGC 6 cut(s) 332, 867, 870, 1086, 1134, 1374
GsuI CTGGAG 1 cut(s) 445
HaeIII GGCC 4 cut(s) 409, 765, 882, 1428
HapII CCGG 2 cut(s) 613, 797
HhaI GCGC 1 cut(s) 1282
Hin1II CATG 9 cut(s) 53, 123, 445, 715, 1028, 1133, 1217, 1236, 1535
Hin6I GCGC 1 cut(s) 1280
HinP1I GCGC 1 cut(s) 1280
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 5 cut(s) 398, 566, 935, 1101, 1556
HpaII CCGG 2 cut(s) 613, 797
HphI GGTGA 2 cut(s) 488, 1040
Hpy166II GTNNAC 3 cut(s) 467, 734, 1706
Hpy188I TCNGA 6 cut(s) 184, 563, 718, 1035, 1255, 1561
Hpy8I GTNNAC 3 cut(s) 467, 734, 1706
HpyAV CCTTC 3 cut(s) 331, 1159, 1472
HpyCH4III ACNGT 1 cut(s) 1550
HpyCH4IV ACGT 2 cut(s) 152, 1594
HpyF10VI GCNNNNNNNGC 3 cut(s) 875, 1207, 1277
HpyF3I CTNAG 5 cut(s) 39, 779, 975, 1019, 1558
HpySE526I ACGT 2 cut(s) 152, 1594
Hsp92II CATG 9 cut(s) 53, 123, 445, 715, 1028, 1133, 1217, 1236, 1535
HspAI GCGC 1 cut(s) 1280
Kpn2I TCCGGA 1 cut(s) 796
KpnI GGTACC 2 cut(s) 787, 1183
Ksp22I TGATCA 2 cut(s) 1030, 1537
Kzo9I GATC 6 cut(s) 131, 390, 1030, 1035, 1537, 1621
LguI GCTCTTC 1 cut(s) 689
LmnI GCTCC 5 cut(s) 29, 276, 438, 1138, 1648
Lsp1109I GCAGC 6 cut(s) 343, 853, 881, 1097, 1120, 1385
LweI GCATC 9 cut(s) 188, 199, 228, 258, 1188, 1258, 1422, 1518, 1621
MaeI CTAG 6 cut(s) 17, 629, 725, 1082, 1401, 1580
MaeII ACGT 2 cut(s) 152, 1594
MaeIII GTNAC 6 cut(s) 286, 526, 602, 683, 1453, 1684
MalI GATC 6 cut(s) 133, 392, 1032, 1037, 1539, 1623
MboI GATC 6 cut(s) 131, 390, 1030, 1035, 1537, 1621
MboII GAAGA 9 cut(s) 247, 320, 358, 672, 706, 1258, 1537, 1616, 1631
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 2 cut(s) 1035, 1621
MhlI GDGCHC 1 cut(s) 780
MlsI TGGCCA 1 cut(s) 882
MluNI TGGCCA 1 cut(s) 882
MlyI GAGTC 1 cut(s) 1550
MmeI TCCRAC 1 cut(s) 1298
Mox20I TGGCCA 1 cut(s) 882
Mph1103I ATGCAT 1 cut(s) 1533
MroI TCCGGA 1 cut(s) 796
MroXI GAANNNNTTC 2 cut(s) 1293, 1623
MscI TGGCCA 1 cut(s) 882
MseI TTAA 6 cut(s) 654, 951, 1076, 1394, 1439, 1743
MslI CAYNNNNRTG 4 cut(s) 323, 501, 752, 1657
Msp20I TGGCCA 1 cut(s) 882
MspA1I CMGCKG 1 cut(s) 334
MspI CCGG 2 cut(s) 613, 797
MspR9I CCNGG 4 cut(s) 614, 822, 1040, 1064
MunI CAATTG 1 cut(s) 873
MvaI CCWGG 3 cut(s) 822, 1040, 1064
MwoI GCNNNNNNNGC 3 cut(s) 875, 1207, 1277
NciI CCSGG 1 cut(s) 614
NcoI CCATGG 1 cut(s) 1024
NdeII GATC 6 cut(s) 131, 390, 1030, 1035, 1537, 1621
NheI GCTAGC 1 cut(s) 1081
NlaIII CATG 9 cut(s) 53, 123, 445, 715, 1028, 1133, 1217, 1236, 1535
NlaIV GGNNCC 6 cut(s) 611, 785, 1181, 1211, 1585, 1650
NmuCI GTSAC 2 cut(s) 683, 1684
NsiI ATGCAT 1 cut(s) 1533
NspI RCATGY 4 cut(s) 445, 715, 1236, 1535
NspV TTCGAA 1 cut(s) 1598
OliI CACNNNNGTG 2 cut(s) 323, 501
PaeI GCATGC 1 cut(s) 1535
PaeR7I CTCGAG 1 cut(s) 168
PaqCI CACCTGC 1 cut(s) 344
PciI ACATGT 2 cut(s) 711, 1232
PciSI GCTCTTC 1 cut(s) 689
PdmI GAANNNNTTC 2 cut(s) 1293, 1623
PfeI GAWTC 4 cut(s) 398, 566, 935, 1101
PflMI CCANNNNNTGG 1 cut(s) 827
PfoI TCCNGGA 2 cut(s) 820, 1038
PkrI GCNGC 6 cut(s) 333, 868, 871, 1087, 1135, 1375
PleI GAGTC 1 cut(s) 1550
PpsI GAGTC 1 cut(s) 1550
PpuMI RGGWCCY 1 cut(s) 1583
PscI ACATGT 2 cut(s) 711, 1232
PsiI TTATAA 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 780
Psp1406I AACGTT 1 cut(s) 1594
Psp5II RGGWCCY 1 cut(s) 1583
Psp6I CCWGG 3 cut(s) 820, 1038, 1062
PspGI CCWGG 3 cut(s) 820, 1038, 1062
PspN4I GGNNCC 6 cut(s) 611, 785, 1181, 1211, 1585, 1650
PspPI GGNCC 4 cut(s) 407, 764, 1427, 1583
PspPPI RGGWCCY 1 cut(s) 1583
PspXI VCTCGAGB 1 cut(s) 168
PsrI GAACNNNNNNTAC 2 cut(s) 602, 634
PstI CTGCAG 2 cut(s) 772, 871
PstNI CAGNNNCTG 1 cut(s) 1039
PsuI RGATCY 2 cut(s) 1035, 1621
PvuII CAGCTG 1 cut(s) 334
RsaI GTAC 9 cut(s) 22, 59, 552, 620, 785, 1004, 1181, 1568, 1707
RsaNI GTAC 9 cut(s) 21, 58, 551, 619, 784, 1003, 1180, 1567, 1706
RseI CAYNNNNRTG 4 cut(s) 323, 501, 752, 1657
SacI GAGCTC 1 cut(s) 780
SapI GCTCTTC 1 cut(s) 689
SaqAI TTAA 6 cut(s) 654, 951, 1076, 1394, 1439, 1743
SatI GCNGC 6 cut(s) 332, 867, 870, 1086, 1134, 1374
Sau3AI GATC 6 cut(s) 131, 390, 1030, 1035, 1537, 1621
Sau96I GGNCC 4 cut(s) 407, 764, 1427, 1583
SbfI CCTGCAGG 1 cut(s) 772
SchI GAGTC 1 cut(s) 1550
ScrFI CCNGG 4 cut(s) 614, 822, 1040, 1064
SdaI CCTGCAGG 1 cut(s) 772
SduI GDGCHC 1 cut(s) 780
SfaNI GCATC 9 cut(s) 188, 199, 228, 258, 1188, 1258, 1422, 1518, 1621
SfcI CTRYAG 5 cut(s) 700, 768, 867, 939, 1188
Sfr274I CTCGAG 1 cut(s) 168
SfuI TTCGAA 1 cut(s) 1598
SinI GGWCC 1 cut(s) 1583
SlaI CTCGAG 1 cut(s) 168
SmiMI CAYNNNNRTG 4 cut(s) 323, 501, 752, 1657
SmlI CTYRAG 3 cut(s) 168, 804, 1284
SmoI CTYRAG 3 cut(s) 168, 804, 1284
SphI GCATGC 1 cut(s) 1535
Sse8387I CCTGCAGG 1 cut(s) 772
SsiI CCGC 3 cut(s) 1136, 1146, 1576
SspMI CTAG 6 cut(s) 17, 629, 725, 1082, 1401, 1580
SstI GAGCTC 1 cut(s) 780
StyD4I CCNGG 4 cut(s) 612, 820, 1038, 1062
StyI CCWWGG 3 cut(s) 1024, 1579, 1587
TaaI ACNGT 1 cut(s) 1550
TaiI ACGT 2 cut(s) 155, 1597
TaqI TCGA 3 cut(s) 169, 569, 1598
TatI WGTACW 2 cut(s) 1566, 1705
TfiI GAWTC 4 cut(s) 398, 566, 935, 1101
Tru1I TTAA 6 cut(s) 654, 951, 1076, 1394, 1439, 1743
Tru9I TTAA 6 cut(s) 654, 951, 1076, 1394, 1439, 1743
TscAI CASTG 3 cut(s) 1112, 1650, 1693
TseFI GTSAC 2 cut(s) 683, 1684
TseI GCWGC 6 cut(s) 331, 866, 869, 1085, 1133, 1373
Tsp45I GTSAC 2 cut(s) 683, 1684
TspDTI ATGAA 7 cut(s) 35, 145, 1059, 1131, 1259, 1489, 1491
TspRI CASTG 3 cut(s) 1112, 1650, 1693
Van91I CCANNNNNTGG 1 cut(s) 827
VpaK11BI GGWCC 1 cut(s) 1583
XapI RAATTY 4 cut(s) 186, 833, 1225, 1495
XbaI TCTAGA 1 cut(s) 724
XceI RCATGY 4 cut(s) 445, 715, 1236, 1535
XhoI CTCGAG 1 cut(s) 168
XmaJI CCTAGG 1 cut(s) 1579
XmnI GAANNNNTTC 2 cut(s) 1293, 1623
XspI CTAG 6 cut(s) 17, 629, 725, 1082, 1401, 1580
Zsp2I ATGCAT 1 cut(s) 1533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.