Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0015669

Physical Location & Seq

Genomic Coordinates Reverse (-)

9772473 .. 9775463

Gene Structure 2991 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1126700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 792 bp

ATGGTAATGGGGAATGCCAGTGGTAGCAGAGATGGTGGAGAAGGCCCTTCTCGAGTTAACAACTTTGAAGAGTACAGTGATGGATATGTAGAAGAATCCATGGCTCAGACTCCATCGCTTTTCTTCACTTCCCAATATCCTGGGGAACCTTTACCAAGACCTGCCGAGGCATTACCGCAACATACAGTATTTAATCGTAATGTACACGATGAGAAATTGATGCGGGCGAAGATCTCATGGAATCACGGTGGCAGGCAAGTTGCTATCACAGGATCATGGGACAATTGGGAGACCAGGGAGCTCTTGCAGAGTATAGGCAATGATTTCTTTATCGTAAAGTTGCTTCCATCAGGTCTCTACCAATACCGATTCATTGTTGACGGGTGTTTGATGTGTGCTCCAGACTTGCCGTGGGTTTATGATAATTCTGGGAGTGCGTATAATATCTTGGACTTGCAGGAATGTATTTCAGAATTGCCTCAGCATCTGGAAAAGTTCGAACCTCCTCCATCTCCACCTTCAAGCTACGACAACAGACTCTTAAGCGAAGATGATTTCAGTAAACCTCCGCTGGAAGTACCCCCACAACTACAAGAATCATTTCTAAACAAGCCATCATCCTCCAACAACGGTGACCAGTCATCACCAATGCCTCGTCCTTCACAATTGAACCATTTGCACATACAGAACCAAATTGGCGGCGAGTTTTTTGCACTCAGCGCTACACGTAGGTTTCATACAAAATTTGTCACAACGGTGTTATACAAGCCATTGAGAAGAACAAATCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04068 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 map04068 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410

Pfam Domains

Protein Families

AMPK1_CBM AMPKBI

Protein Analysis

264

Amino Acids

29.75

Weight (kDa)

5.27

Isoelectric Point (pI)

60.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMPK1_CBM	PF16561	76 - 154	2.2e-24	Glycogen recognition site of AMP-activated protein kinase
AMPKBI	PF04739	187 - 257	6.5e-19	5'-AMP-activated protein kinase beta subunit, interaction domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015669)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40620
malus_domestica	MD09G1126700.v1.1 MD17G1117000.v1.1
prunus_persica	Prupe.3G197500_v2.0.a1 Prupe.3G197500_v2.0.a1
pyrus_communis	pycom09g05090
rosa_chinensis	RchiOBHm_Chr2g0155991
rosa_laevigata	RLG00000020879
rosa_multiflora	Rmu_sc0000260.1_g000025
rosa_roxburghii	Rroxscaffold_2G00093310
rosa_rugosa	Rorug02G0451000
rosa_samantha	Rh2AG517800 Rh2BG529000 Rh2CG502500 Rh2DG538100
rosa_wichuraiana	Rw2G042630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	169
AciI	CCGC	4 cut(s)	176, 223, 569, 699
AclWI	GGATC	1 cut(s)	280
AcsI	RAATTY	1 cut(s)	743
AfaI	GTAC	3 cut(s)	74, 204, 579
AfeI	AGCGCT	1 cut(s)	721
AflII	CTTAAG	1 cut(s)	541
AflIII	ACRYGT	1 cut(s)	725
AgsI	TTSAA	3 cut(s)	68, 522, 670
AjnI	CCWGG	2 cut(s)	139, 293
AleI	CACNNNNGTG	1 cut(s)	755
AluBI	AGCT	2 cut(s)	301, 525
AluI	AGCT	2 cut(s)	301, 525
Alw21I	GWGCWC	2 cut(s)	303, 400
Alw26I	GTCTC	2 cut(s)	284, 359
AlwI	GGATC	1 cut(s)	280
AlwNI	CAGNNNCTG	1 cut(s)	487
Ama87I	CYCGRG	1 cut(s)	51
Aor51HI	AGCGCT	1 cut(s)	721
AoxI	GGCC	1 cut(s)	43
ApoI	RAATTY	1 cut(s)	743
Asp700I	GAANNNNTTC	1 cut(s)	600
AspLEI	GCGC	1 cut(s)	722
AspS9I	GGNCC	1 cut(s)	44
AsuHPI	GGTGA	2 cut(s)	636, 644
AsuII	TTCGAA	1 cut(s)	498
AvaI	CYCGRG	1 cut(s)	51
BanII	GRGCYC	1 cut(s)	303
BarI	GAAGNNNNNNTAC	2 cut(s)	327, 359
Bbv12I	GWGCWC	2 cut(s)	303, 400
BbvCI	CCTCAGC	1 cut(s)	480
BccI	CCATC	6 cut(s)	26, 74, 121, 355, 517, 622
BceAI	ACGGC	1 cut(s)	394
BcgI	CGANNNNNNTGC	2 cut(s)	692, 726
BciT130I	CCWGG	2 cut(s)	141, 295
BcoDI	GTCTC	2 cut(s)	284, 359
BfoI	RGCGCY	1 cut(s)	723
BfrI	CTTAAG	1 cut(s)	541
BfuAI	ACCTGC	1 cut(s)	169
BglII	AGATCT	1 cut(s)	231
BisI	GCNGC	1 cut(s)	700
BlsI	GCNGC	1 cut(s)	701
Bme1390I	CCNGG	2 cut(s)	141, 295
BmeT110I	CYCGRG	1 cut(s)	51
BmgT120I	GGNCC	1 cut(s)	44
BmiI	GGNNCC	1 cut(s)	147
BmrFI	CCNGG	2 cut(s)	141, 295
BmsI	GCATC	2 cut(s)	210, 493
BpmI	CTGGAG	1 cut(s)	384
Bpu10I	CCTNAGC	1 cut(s)	480
Bpu14I	TTCGAA	1 cut(s)	498
BsaAI	YACGTR	1 cut(s)	728
BsaI	GGTCTC	2 cut(s)	284, 359
BsaJI	CCNNGG	5 cut(s)	99, 140, 165, 294, 410
Bse1I	ACTGG	2 cut(s)	18, 637
Bse3DI	GCAATG	1 cut(s)	325
BseBI	CCWGG	2 cut(s)	141, 295
BseDI	CCNNGG	5 cut(s)	99, 140, 165, 294, 410
BseGI	GGATG	1 cut(s)	617
BseMI	GCAATG	1 cut(s)	325
BseMII	CTCAG	3 cut(s)	119, 494, 730
BseNI	ACTGG	2 cut(s)	18, 637
BseRI	GAGGAG	1 cut(s)	495
BshFI	GGCC	1 cut(s)	45
BsiHKAI	GWGCWC	2 cut(s)	303, 400
BsiHKCI	CYCGRG	1 cut(s)	51
BslFI	GGGAC	1 cut(s)	293
BsmAI	GTCTC	2 cut(s)	284, 359
BsmFI	GGGAC	1 cut(s)	293
BsmI	GAATGC	1 cut(s)	19
BsnI	GGCC	1 cut(s)	45
Bso31I	GGTCTC	2 cut(s)	284, 359
BsoBI	CYCGRG	1 cut(s)	51
Bsp119I	TTCGAA	1 cut(s)	498
Bsp1286I	GDGCHC	2 cut(s)	303, 400
Bsp1407I	TGTACA	1 cut(s)	202
Bsp143I	GATC	2 cut(s)	231, 272
Bsp19I	CCATGG	1 cut(s)	99
BspACI	CCGC	4 cut(s)	176, 223, 569, 699
BspANI	GGCC	1 cut(s)	45
BspCNI	CTCAG	3 cut(s)	118, 493, 729
BspLI	GGNNCC	1 cut(s)	147
BspMI	ACCTGC	1 cut(s)	169
BspPI	GGATC	1 cut(s)	280
BspT104I	TTCGAA	1 cut(s)	498
BspTI	CTTAAG	1 cut(s)	541
BspTNI	GGTCTC	2 cut(s)	284, 359
BsrDI	GCAATG	1 cut(s)	325
BsrGI	TGTACA	1 cut(s)	202
BsrI	ACTGG	2 cut(s)	18, 637
BssECI	CCNNGG	5 cut(s)	99, 140, 165, 294, 410
BssMI	GATC	2 cut(s)	231, 272
BssT1I	CCWWGG	1 cut(s)	99
Bst2UI	CCWGG	2 cut(s)	141, 295
Bst4CI	ACNGT	5 cut(s)	77, 187, 248, 632, 757
Bst6I	CTCTTC	1 cut(s)	63
BstAFI	CTTAAG	1 cut(s)	541
BstAUI	TGTACA	1 cut(s)	202
BstBAI	YACGTR	1 cut(s)	728
BstBI	TTCGAA	1 cut(s)	498
BstC8I	GCNNGC	2 cut(s)	225, 254
BstDEI	CTNAG	3 cut(s)	105, 480, 716
BstDSI	CCRYGG	2 cut(s)	99, 410
BstEII	GGTNACC	1 cut(s)	632
BstF5I	GGATG	1 cut(s)	617
BstH2I	RGCGCY	1 cut(s)	723
BstHHI	GCGC	1 cut(s)	722
BstKTI	GATC	2 cut(s)	234, 275
BstMAI	GTCTC	2 cut(s)	284, 359
BstMBI	GATC	2 cut(s)	231, 272
BstMWI	GCNNNNNNNGC	1 cut(s)	719
BstNI	CCWGG	2 cut(s)	141, 295
BstPI	GGTNACC	1 cut(s)	632
BstSCI	CCNGG	2 cut(s)	139, 293
BstX2I	RGATCY	1 cut(s)	231
BstXI	CCANNNNNNTGG	1 cut(s)	140
BstYI	RGATCY	1 cut(s)	231
BsuRI	GGCC	1 cut(s)	45
BtgI	CCRYGG	2 cut(s)	99, 410
BtgZI	GCGATG	1 cut(s)	99
BtsCI	GGATG	1 cut(s)	617
BtsIMutI	CAGTG	2 cut(s)	25, 82
BveI	ACCTGC	1 cut(s)	169
Cac8I	GCNNGC	2 cut(s)	225, 254
CaiI	CAGNNNCTG	1 cut(s)	487
CfoI	GCGC	1 cut(s)	722
Cfr13I	GGNCC	1 cut(s)	44
Csp6I	GTAC	3 cut(s)	73, 203, 578
CviAII	CATG	3 cut(s)	100, 237, 276
CviJI	RGCY	6 cut(s)	45, 104, 301, 525, 613, 769
CviKI_1	RGCY	6 cut(s)	45, 104, 301, 525, 613, 769
CviQI	GTAC	3 cut(s)	73, 203, 578
DdeI	CTNAG	3 cut(s)	105, 480, 716
DpnI	GATC	2 cut(s)	233, 274
DpnII	GATC	2 cut(s)	231, 272
Eam1104I	CTCTTC	1 cut(s)	63
EarI	CTCTTC	1 cut(s)	63
Ecl136II	GAGCTC	1 cut(s)	301
Eco130I	CCWWGG	1 cut(s)	99
Eco24I	GRGCYC	1 cut(s)	303
Eco31I	GGTCTC	2 cut(s)	284, 359
Eco47III	AGCGCT	1 cut(s)	721
Eco53kI	GAGCTC	1 cut(s)	301
Eco88I	CYCGRG	1 cut(s)	51
Eco91I	GGTNACC	1 cut(s)	632
EcoICRI	GAGCTC	1 cut(s)	301
EcoO109I	RGGNCCY	1 cut(s)	44
EcoO65I	GGTNACC	1 cut(s)	632
EcoRII	CCWGG	2 cut(s)	139, 293
EcoT14I	CCWWGG	1 cut(s)	99
EcoT38I	GRGCYC	1 cut(s)	303
ErhI	CCWWGG	1 cut(s)	99
FaeI	CATG	3 cut(s)	103, 240, 279
FaqI	GGGAC	1 cut(s)	293
FatI	CATG	3 cut(s)	99, 236, 275
FauI	CCCGC	1 cut(s)	216
Fnu4HI	GCNGC	1 cut(s)	700
FokI	GGATG	1 cut(s)	604
FriOI	GRGCYC	1 cut(s)	303
Fsp4HI	GCNGC	1 cut(s)	700
GlaI	GCGC	1 cut(s)	721
GluI	GCNGC	1 cut(s)	700
GsuI	CTGGAG	1 cut(s)	384
HaeII	RGCGCY	1 cut(s)	723
HaeIII	GGCC	1 cut(s)	45
HhaI	GCGC	1 cut(s)	722
Hin1II	CATG	3 cut(s)	103, 240, 279
Hin6I	GCGC	1 cut(s)	720
HinP1I	GCGC	1 cut(s)	720
HincII	GTYRAC	2 cut(s)	58, 379
HindII	GTYRAC	2 cut(s)	58, 379
HinfI	GANTC	6 cut(s)	95, 109, 241, 369, 537, 596
HpaI	GTTAAC	1 cut(s)	58
HphI	GGTGA	2 cut(s)	636, 644
Hpy166II	GTNNAC	4 cut(s)	58, 205, 379, 563
Hpy188I	TCNGA	2 cut(s)	108, 472
Hpy188III	TCNNGA	3 cut(s)	51, 401, 488
Hpy8I	GTNNAC	4 cut(s)	58, 205, 379, 563
HpyAV	CCTTC	4 cut(s)	35, 57, 528, 669
HpyCH4III	ACNGT	5 cut(s)	77, 187, 248, 632, 757
HpyCH4IV	ACGT	1 cut(s)	727
HpyCH4V	TGCA	4 cut(s)	307, 457, 679, 713
HpyF10VI	GCNNNNNNNGC	1 cut(s)	719
HpyF3I	CTNAG	3 cut(s)	105, 480, 716
HpySE526I	ACGT	1 cut(s)	727
Hsp92II	CATG	3 cut(s)	103, 240, 279
HspAI	GCGC	1 cut(s)	720
KspAI	GTTAAC	1 cut(s)	58
Kzo9I	GATC	2 cut(s)	231, 272
LmnI	GCTCC	2 cut(s)	298, 403
LweI	GCATC	2 cut(s)	210, 493
MaeII	ACGT	1 cut(s)	727
MaeIII	GTNAC	2 cut(s)	632, 748
MalI	GATC	2 cut(s)	233, 274
MboI	GATC	2 cut(s)	231, 272
MboII	GAAGA	6 cut(s)	80, 104, 115, 241, 560, 789
MfeI	CAATTG	2 cut(s)	283, 665
MflI	RGATCY	1 cut(s)	231
MhlI	GDGCHC	2 cut(s)	303, 400
MluCI	AATT	7 cut(s)	215, 283, 424, 473, 665, 693, 743
MlyI	GAGTC	2 cut(s)	103, 531
MmeI	TCCRAC	1 cut(s)	648
MnlI	CCTC	7 cut(s)	160, 489, 513, 516, 576, 631, 663
MroXI	GAANNNNTTC	1 cut(s)	600
MseI	TTAA	3 cut(s)	57, 192, 542
MslI	CAYNNNNRTG	1 cut(s)	755
MspA1I	CMGCKG	1 cut(s)	571
MspCI	CTTAAG	1 cut(s)	541
MspR9I	CCNGG	2 cut(s)	141, 295
MunI	CAATTG	2 cut(s)	283, 665
Mva1269I	GAATGC	1 cut(s)	19
MvaI	CCWGG	2 cut(s)	141, 295
MwoI	GCNNNNNNNGC	1 cut(s)	719
NcoI	CCATGG	1 cut(s)	99
NdeII	GATC	2 cut(s)	231, 272
NlaIII	CATG	3 cut(s)	103, 240, 279
NlaIV	GGNNCC	1 cut(s)	147
NmeAIII	GCCGAG	1 cut(s)	190
NmuCI	GTSAC	2 cut(s)	632, 748
NspV	TTCGAA	1 cut(s)	498
OliI	CACNNNNGTG	1 cut(s)	755
PaeR7I	CTCGAG	1 cut(s)	51
PctI	GAATGC	1 cut(s)	19
PdmI	GAANNNNTTC	1 cut(s)	600
PfeI	GAWTC	4 cut(s)	95, 241, 369, 596
PkrI	GCNGC	1 cut(s)	701
PleI	GAGTC	2 cut(s)	103, 531
PpsI	GAGTC	2 cut(s)	103, 531
Ppu21I	YACGTR	1 cut(s)	728
Psp124BI	GAGCTC	1 cut(s)	303
Psp6I	CCWGG	2 cut(s)	139, 293
PspEI	GGTNACC	1 cut(s)	632
PspGI	CCWGG	2 cut(s)	139, 293
PspN4I	GGNNCC	1 cut(s)	147
PspPI	GGNCC	1 cut(s)	44
PstNI	CAGNNNCTG	1 cut(s)	487
PsuI	RGATCY	1 cut(s)	231
RsaI	GTAC	3 cut(s)	74, 204, 579
RsaNI	GTAC	3 cut(s)	73, 203, 578
RseI	CAYNNNNRTG	1 cut(s)	755
SacI	GAGCTC	1 cut(s)	303
SaqAI	TTAA	3 cut(s)	57, 192, 542
SatI	GCNGC	1 cut(s)	700
Sau3AI	GATC	2 cut(s)	231, 272
Sau96I	GGNCC	1 cut(s)	44
SchI	GAGTC	2 cut(s)	103, 531
ScrFI	CCNGG	2 cut(s)	141, 295
SduI	GDGCHC	2 cut(s)	303, 400
SfaNI	GCATC	2 cut(s)	210, 493
Sfr274I	CTCGAG	1 cut(s)	51
SfuI	TTCGAA	1 cut(s)	498
SlaI	CTCGAG	1 cut(s)	51
SmiMI	CAYNNNNRTG	1 cut(s)	755
SmlI	CTYRAG	2 cut(s)	51, 541
SmoI	CTYRAG	2 cut(s)	51, 541
Sse9I	AATT	7 cut(s)	215, 283, 424, 473, 665, 693, 743
SsiI	CCGC	4 cut(s)	176, 223, 569, 699
SstI	GAGCTC	1 cut(s)	303
StyD4I	CCNGG	2 cut(s)	139, 293
StyI	CCWWGG	1 cut(s)	99
TaaI	ACNGT	5 cut(s)	77, 187, 248, 632, 757
TaiI	ACGT	1 cut(s)	730
TaqI	TCGA	2 cut(s)	52, 498
TasI	AATT	7 cut(s)	215, 283, 424, 473, 665, 693, 743
TatI	WGTACW	2 cut(s)	72, 202
TauI	GCSGC	1 cut(s)	702
TfiI	GAWTC	4 cut(s)	95, 241, 369, 596
Tru1I	TTAA	3 cut(s)	57, 192, 542
Tru9I	TTAA	3 cut(s)	57, 192, 542
TscAI	CASTG	2 cut(s)	25, 82
TseFI	GTSAC	2 cut(s)	632, 748
Tsp45I	GTSAC	2 cut(s)	632, 748
TspDTI	ATGAA	2 cut(s)	361, 725
TspRI	CASTG	2 cut(s)	25, 82
Vha464I	CTTAAG	1 cut(s)	541
XapI	RAATTY	1 cut(s)	743
XcmI	CCANNNNNNNNNTGG	1 cut(s)	408
XhoI	CTCGAG	1 cut(s)	51
XmnI	GAANNNNTTC	1 cut(s)	600

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.