Rroxscaffold_2G00093310

SNF1-related protein kinase regulatory subunit beta-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015669
Physical Location & Seq
Reverse (-)
14700609 .. 14703884
3276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00093310.1

Sequence Viewer

Length: 810 bp
ATGGTAATGGGTAATGCCAGTGGTAGAGAGGAAGGAGAAGGCTCTTCTTCTGGAGTTAATAACTTTCAAGGGAATGATGAATCCATGGCTCCCTCACCTCCCCATAACCCCTTCTTCACTTCACAAAATCCTGGGGACACGTTAACAAGTCCGGGTGGGGCAATACAGACTCAAACTCATGCGTTATCGCAAAATATAGAATATAATGATAATGTACAAAATCAGAACTTGAGACGCATTAAGATCTCATGGACTCATGGTGGCAGGCAAGTTGCTATCACAGGATCATGGGACAATTGGCAGACCAGGGAGCCGCTGCAGAATTTAGGTAACGAATTCGTTGTTGTCATAAAGTTGCTTCCATCAGGTTTCTATCAGTACCGCTTCATTGTTGATGGGTGTTGGAGGTGTGCTCCAGACTTGCCATGGATCTATGATGATTCGGGGACTGCCTATAATGTTTTGGATTTGCAGGAATATGGTTTGGAATTGCCTGAACGCCTGTCAAAGTTCGAATCTCCTCCATCCCCACCTTCAAGCTATGATAACAGATGCTTAAGTGAAAATGATTTCAGTAAACCTCCGCCAGAAGTACCACCACAACTACAAGTCCCATTTTTAAACAGGCCACCCTCCTCTTCAAATGATGGTGACCGGCCATTTTCAATGCCTCATTATTCACAATTGAACCATTTGTACATCCAGAACCAAATTGGTGGCGAGTATTTCGCACTCAGTTCTACACATAGGTTTCATAAAAAATTTGTTACAATGGTGTTATACAAGCCATTGAACAGAGAAAATCCATAA

Protein Analysis

269

Amino Acids

30.34

Weight (kDa)

5.34

Isoelectric Point (pI)

57.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPK1_CBM PF16561 80 - 159 9.5e-27 Glycogen recognition site of AMP-activated protein kinase
AMPKBI PF04739 192 - 263 3.5e-19 5'-AMP-activated protein kinase beta subunit, interaction domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015669)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g40620
malus_domestica MD09G1126700.v1.1 MD17G1117000.v1.1
prunus_persica Prupe.3G197500_v2.0.a1 Prupe.3G197500_v2.0.a1
pyrus_communis pycom09g05090
rosa_chinensis RchiOBHm_Chr2g0155991
rosa_laevigata RLG00000020879
rosa_multiflora Rmu_sc0000260.1_g000025
rosa_roxburghii Rroxscaffold_2G00093310
rosa_rugosa Rorug02G0451000
rosa_samantha Rh2AG517800 Rh2BG529000 Rh2CG502500 Rh2DG538100
rosa_wichuraiana Rw2G042630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 314, 382, 584
AclWI GGATC 2 cut(s) 292, 437
AcoI YGGCCR 1 cut(s) 656
AcsI RAATTY 3 cut(s) 322, 335, 761
AfaI GTAC 4 cut(s) 216, 380, 594, 698
AflII CTTAAG 1 cut(s) 556
AflIII ACRYGT 1 cut(s) 138
AgsI TTSAA 6 cut(s) 68, 537, 642, 666, 688, 793
AjnI CCWGG 2 cut(s) 130, 305
AluBI AGCT 1 cut(s) 540
AluI AGCT 1 cut(s) 540
Alw21I GWGCWC 1 cut(s) 415
Alw26I GTCTC 1 cut(s) 226
AlwI GGATC 2 cut(s) 292, 437
AoxI GGCC 2 cut(s) 626, 656
ApeKI GCWGC 1 cut(s) 316
ApoI RAATTY 3 cut(s) 322, 335, 761
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 2 cut(s) 87, 662
AsuII TTCGAA 1 cut(s) 513
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 1 cut(s) 303
BccI CCATC 4 cut(s) 370, 389, 532, 641
BciT130I CCWGG 2 cut(s) 132, 307
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 1 cut(s) 226
BfmI CTRYAG 1 cut(s) 317
BfrI CTTAAG 1 cut(s) 556
BglII AGATCT 1 cut(s) 243
BisI GCNGC 2 cut(s) 314, 317
BlsI GCNGC 2 cut(s) 315, 318
Bme1390I CCNGG 3 cut(s) 132, 153, 307
BmiI GGNNCC 2 cut(s) 90, 312
BmrFI CCNGG 3 cut(s) 132, 153, 307
BmsI GCATC 1 cut(s) 542
BplI GAGNNNNNCTC 2 cut(s) 397, 429
BpmI CTGGAG 2 cut(s) 72, 399
Bpu14I TTCGAA 1 cut(s) 513
BpuEI CTTGAG 1 cut(s) 250
BpuMI CCSGG 1 cut(s) 153
BsaJI CCNNGG 4 cut(s) 84, 131, 306, 425
Bse118I RCCGGY 1 cut(s) 654
Bse1I ACTGG 1 cut(s) 18
BseBI CCWGG 2 cut(s) 132, 307
BseDI CCNNGG 4 cut(s) 84, 131, 306, 425
BseGI GGATG 2 cut(s) 524, 699
BseMII CTCAG 1 cut(s) 748
BseNI ACTGG 1 cut(s) 18
BseRI GAGGAG 2 cut(s) 510, 625
BseXI GCAGC 1 cut(s) 303
BshFI GGCC 2 cut(s) 628, 658
BsiHKAI GWGCWC 1 cut(s) 415
BsiSI CCGG 2 cut(s) 152, 655
BslFI GGGAC 4 cut(s) 149, 305, 460, 596
BsmAI GTCTC 1 cut(s) 226
BsmBI CGTCTC 1 cut(s) 226
BsmFI GGGAC 4 cut(s) 149, 305, 460, 596
BsnI GGCC 2 cut(s) 628, 658
Bsp119I TTCGAA 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 415
Bsp1407I TGTACA 2 cut(s) 214, 696
Bsp143I GATC 3 cut(s) 243, 284, 429
Bsp19I CCATGG 2 cut(s) 84, 425
BspACI CCGC 3 cut(s) 314, 382, 584
BspANI GGCC 2 cut(s) 628, 658
BspCNI CTCAG 1 cut(s) 747
BspLI GGNNCC 2 cut(s) 90, 312
BspMAI CTGCAG 1 cut(s) 321
BspPI GGATC 2 cut(s) 292, 437
BspQI GCTCTTC 1 cut(s) 49
BspT104I TTCGAA 1 cut(s) 513
BspTI CTTAAG 1 cut(s) 556
BsrFI RCCGGY 1 cut(s) 654
BsrGI TGTACA 2 cut(s) 214, 696
BsrI ACTGG 1 cut(s) 18
BssAI RCCGGY 1 cut(s) 654
BssECI CCNNGG 4 cut(s) 84, 131, 306, 425
BssMI GATC 3 cut(s) 243, 284, 429
BssT1I CCWWGG 2 cut(s) 84, 425
Bst2UI CCWGG 2 cut(s) 132, 307
Bst6I CTCTTC 2 cut(s) 49, 643
BstAFI CTTAAG 1 cut(s) 556
BstAUI TGTACA 2 cut(s) 214, 696
BstBI TTCGAA 1 cut(s) 513
BstC8I GCNNGC 1 cut(s) 266
BstDEI CTNAG 1 cut(s) 734
BstDSI CCRYGG 2 cut(s) 84, 425
BstEII GGTNACC 1 cut(s) 650
BstF5I GGATG 2 cut(s) 524, 699
BstKTI GATC 3 cut(s) 246, 287, 432
BstMAI GTCTC 1 cut(s) 226
BstMBI GATC 3 cut(s) 243, 284, 429
BstNI CCWGG 2 cut(s) 132, 307
BstPI GGTNACC 1 cut(s) 650
BstSCI CCNGG 3 cut(s) 130, 151, 305
BstSFI CTRYAG 1 cut(s) 317
BstV1I GCAGC 1 cut(s) 303
BstX2I RGATCY 2 cut(s) 243, 429
BstXI CCANNNNNNTGG 1 cut(s) 716
BstYI RGATCY 2 cut(s) 243, 429
BsuRI GGCC 2 cut(s) 628, 658
BtgI CCRYGG 2 cut(s) 84, 425
BtsCI GGATG 2 cut(s) 524, 699
BtsIMutI CAGTG 1 cut(s) 25
Cac8I GCNNGC 1 cut(s) 266
Cfr10I RCCGGY 1 cut(s) 654
CseI GACGC 1 cut(s) 243
Csp6I GTAC 4 cut(s) 215, 379, 593, 697
CviAII CATG 6 cut(s) 85, 179, 249, 257, 288, 426
CviJI RGCY 7 cut(s) 42, 89, 313, 540, 628, 658, 787
CviKI_1 RGCY 7 cut(s) 42, 89, 313, 540, 628, 658, 787
CviQI GTAC 4 cut(s) 215, 379, 593, 697
DdeI CTNAG 1 cut(s) 734
DpnI GATC 3 cut(s) 245, 286, 431
DpnII GATC 3 cut(s) 243, 284, 429
DraI TTTAAA 1 cut(s) 621
EaeI YGGCCR 1 cut(s) 656
Eam1104I CTCTTC 2 cut(s) 49, 643
EarI CTCTTC 2 cut(s) 49, 643
EciI GGCGGA 1 cut(s) 573
Eco130I CCWWGG 2 cut(s) 84, 425
Eco91I GGTNACC 1 cut(s) 650
EcoO65I GGTNACC 1 cut(s) 650
EcoRI GAATTC 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 130, 305
EcoT14I CCWWGG 2 cut(s) 84, 425
ErhI CCWWGG 2 cut(s) 84, 425
Esp3I CGTCTC 1 cut(s) 226
FaeI CATG 6 cut(s) 88, 182, 252, 260, 291, 429
FaqI GGGAC 4 cut(s) 149, 305, 460, 596
FatI CATG 6 cut(s) 84, 178, 248, 256, 287, 425
Fnu4HI GCNGC 2 cut(s) 314, 317
FokI GGATG 2 cut(s) 511, 686
Fsp4HI GCNGC 2 cut(s) 314, 317
GluI GCNGC 2 cut(s) 314, 317
GsuI CTGGAG 2 cut(s) 72, 399
HaeIII GGCC 2 cut(s) 628, 658
HapII CCGG 2 cut(s) 152, 655
HgaI GACGC 1 cut(s) 243
Hin1II CATG 6 cut(s) 88, 182, 252, 260, 291, 429
HincII GTYRAC 1 cut(s) 144
HindII GTYRAC 1 cut(s) 144
HinfI GANTC 5 cut(s) 80, 169, 253, 440, 515
HpaI GTTAAC 1 cut(s) 144
HpaII CCGG 2 cut(s) 152, 655
HphI GGTGA 2 cut(s) 87, 662
Hpy166II GTNNAC 2 cut(s) 144, 578
Hpy188I TCNGA 1 cut(s) 225
Hpy188III TCNNGA 3 cut(s) 51, 416, 703
Hpy8I GTNNAC 2 cut(s) 144, 578
HpyAV CCTTC 4 cut(s) 26, 32, 121, 543
HpyCH4IV ACGT 1 cut(s) 140
HpyCH4V TGCA 2 cut(s) 319, 472
HpyF3I CTNAG 1 cut(s) 734
HpySE526I ACGT 1 cut(s) 140
Hsp92II CATG 6 cut(s) 88, 182, 252, 260, 291, 429
KspAI GTTAAC 1 cut(s) 144
Kzo9I GATC 3 cut(s) 243, 284, 429
LguI GCTCTTC 1 cut(s) 49
LmnI GCTCC 3 cut(s) 94, 310, 418
Lsp1109I GCAGC 1 cut(s) 303
LweI GCATC 1 cut(s) 542
MaeII ACGT 1 cut(s) 140
MaeIII GTNAC 3 cut(s) 329, 650, 766
MalI GATC 3 cut(s) 245, 286, 431
MboI GATC 3 cut(s) 243, 284, 429
MboII GAAGA 4 cut(s) 36, 39, 106, 630
MfeI CAATTG 2 cut(s) 295, 683
MflI RGATCY 2 cut(s) 243, 429
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 7 cut(s) 295, 322, 335, 488, 683, 711, 761
MlyI GAGTC 2 cut(s) 163, 247
MmeI TCCRAC 1 cut(s) 383
MnlI CCTC 9 cut(s) 22, 103, 108, 399, 531, 591, 643, 646, 681
MseI TTAA 5 cut(s) 57, 143, 240, 557, 620
MspA1I CMGCKG 1 cut(s) 316
MspCI CTTAAG 1 cut(s) 556
MspI CCGG 2 cut(s) 152, 655
MspR9I CCNGG 3 cut(s) 132, 153, 307
MunI CAATTG 2 cut(s) 295, 683
MvaI CCWGG 2 cut(s) 132, 307
NciI CCSGG 1 cut(s) 153
NcoI CCATGG 2 cut(s) 84, 425
NdeII GATC 3 cut(s) 243, 284, 429
NlaIII CATG 6 cut(s) 88, 182, 252, 260, 291, 429
NlaIV GGNNCC 2 cut(s) 90, 312
NmuCI GTSAC 1 cut(s) 650
NspV TTCGAA 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 49
PfeI GAWTC 3 cut(s) 80, 440, 515
PkrI GCNGC 2 cut(s) 315, 318
PleI GAGTC 2 cut(s) 163, 247
PpsI GAGTC 2 cut(s) 163, 247
Psp6I CCWGG 2 cut(s) 130, 305
PspEI GGTNACC 1 cut(s) 650
PspGI CCWGG 2 cut(s) 130, 305
PspN4I GGNNCC 2 cut(s) 90, 312
PsrI GAACNNNNNNTAC 2 cut(s) 680, 712
PstI CTGCAG 1 cut(s) 321
PsuI RGATCY 2 cut(s) 243, 429
RsaI GTAC 4 cut(s) 216, 380, 594, 698
RsaNI GTAC 4 cut(s) 215, 379, 593, 697
SapI GCTCTTC 1 cut(s) 49
SaqAI TTAA 5 cut(s) 57, 143, 240, 557, 620
SatI GCNGC 2 cut(s) 314, 317
Sau3AI GATC 3 cut(s) 243, 284, 429
SchI GAGTC 2 cut(s) 163, 247
ScrFI CCNGG 3 cut(s) 132, 153, 307
SduI GDGCHC 1 cut(s) 415
SetI ASST 9 cut(s) 100, 143, 331, 370, 410, 535, 542, 583, 752
SfaNI GCATC 1 cut(s) 542
SfcI CTRYAG 1 cut(s) 317
SfuI TTCGAA 1 cut(s) 513
SmlI CTYRAG 2 cut(s) 229, 556
SmoI CTYRAG 2 cut(s) 229, 556
Sse9I AATT 7 cut(s) 295, 322, 335, 488, 683, 711, 761
SsiI CCGC 3 cut(s) 314, 382, 584
StyD4I CCNGG 3 cut(s) 130, 151, 305
StyI CCWWGG 2 cut(s) 84, 425
TaiI ACGT 1 cut(s) 143
TaqI TCGA 1 cut(s) 513
TasI AATT 7 cut(s) 295, 322, 335, 488, 683, 711, 761
TatI WGTACW 2 cut(s) 214, 696
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 3 cut(s) 80, 440, 515
Tru1I TTAA 5 cut(s) 57, 143, 240, 557, 620
Tru9I TTAA 5 cut(s) 57, 143, 240, 557, 620
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 1 cut(s) 316
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 3 cut(s) 93, 376, 743
TspRI CASTG 1 cut(s) 25
Vha464I CTTAAG 1 cut(s) 556
XapI RAATTY 3 cut(s) 322, 335, 761
XcmI CCANNNNNNNNNTGG 2 cut(s) 423, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.