Rh2CG502500

SNF1-related protein kinase regulatory subunit beta-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0015669
Physical Location & Seq
Forward (+)
67422220 .. 67426408
4189 bp
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UTR
Exon/CDS
Intron
Rh2CG502500.1

Sequence Viewer

Length: 597 bp
ATGATTGCCTCTGTTTTTTTTCTTTTGGATTTTTCTGGTAACTGGGTTCCTTTTCAGTTTTTGTGGTTTTATTGTAAGATGAATCGGAAGATAAATAATGGGTTTCCTTTGTTTTCAGTAATGGGTAATGCCAGTGGTAGAGAGGAAGGAGAAGGCTCTTCTTCTGGAGTTAATAACTTTCAAGAGAATGATGAATCCATGGCTCCCTCACCTCCCCATAACCCCTTCTTCACTTCGGAAAATCCTGGGGACACGTTAACAAGACCGGGTGGGGCAATACAGACTCAAACTCATGCGTTATCGCAAAATATAGAATATAATGATAATGTACAAAATCAGAACTTGAGACGCATGAAGATCTCATGGACTCATGGTGGCAGGCAAGTTGCTATCACAGGATCATGGGACAATTGGCAGACCAGGGAGCCGCTGCAGAATTTAGGTAACGAATTCGTTGTCATAAAGTTGCTTCCATCAGGTTTCTATCAGTACCGCTTCATTGTTGATGGGTGTTGGAGGTGTGCTCCAGACTTGCCATGGATCTATGATGATTCGGGGACTGCCTATAATGTTTTGGATTTGCAGTTGACCAAGTAA

Protein Analysis

198

Amino Acids

22.53

Weight (kDa)

5.12

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPK1_CBM PF16561 118 - 195 8e-28 Glycogen recognition site of AMP-activated protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015669)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g40620
malus_domestica MD09G1126700.v1.1 MD17G1117000.v1.1
prunus_persica Prupe.3G197500_v2.0.a1 Prupe.3G197500_v2.0.a1
pyrus_communis pycom09g05090
rosa_chinensis RchiOBHm_Chr2g0155991
rosa_laevigata RLG00000020879
rosa_multiflora Rmu_sc0000260.1_g000025
rosa_roxburghii Rroxscaffold_2G00093310
rosa_rugosa Rorug02G0451000
rosa_samantha Rh2AG517800 Rh2BG529000 Rh2CG502500 Rh2DG538100
rosa_wichuraiana Rw2G042630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 428, 493
AclWI GGATC 2 cut(s) 406, 548
AcsI RAATTY 2 cut(s) 436, 449
AfaI GTAC 2 cut(s) 330, 491
AflIII ACRYGT 1 cut(s) 252
AgsI TTSAA 1 cut(s) 182
AjnI CCWGG 2 cut(s) 244, 419
Alw21I GWGCWC 1 cut(s) 526
Alw26I GTCTC 1 cut(s) 340
AlwI GGATC 2 cut(s) 406, 548
ApeKI GCWGC 1 cut(s) 430
ApoI RAATTY 2 cut(s) 436, 449
ArsI GACNNNNNNTTYG 2 cut(s) 441, 473
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 526
BbvI GCAGC 1 cut(s) 417
BccI CCATC 2 cut(s) 481, 500
BciT130I CCWGG 2 cut(s) 246, 421
BcnI CCSGG 1 cut(s) 267
BcoDI GTCTC 1 cut(s) 340
BfmI CTRYAG 1 cut(s) 431
BglII AGATCT 1 cut(s) 357
BisI GCNGC 2 cut(s) 428, 431
BlsI GCNGC 2 cut(s) 429, 432
Bme1390I CCNGG 3 cut(s) 246, 267, 421
BmiI GGNNCC 3 cut(s) 48, 204, 426
BmrFI CCNGG 3 cut(s) 246, 267, 421
BmrI ACTGGG 1 cut(s) 52
BmuI ACTGGG 1 cut(s) 52
BplI GAGNNNNNCTC 2 cut(s) 508, 540
BpmI CTGGAG 2 cut(s) 186, 510
BpuEI CTTGAG 1 cut(s) 364
BpuMI CCSGG 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 198, 245, 420, 536
Bse1I ACTGG 2 cut(s) 47, 132
BseBI CCWGG 2 cut(s) 246, 421
BseDI CCNNGG 4 cut(s) 198, 245, 420, 536
BseNI ACTGG 2 cut(s) 47, 132
BseXI GCAGC 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 526
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 3 cut(s) 263, 419, 571
BsmAI GTCTC 1 cut(s) 340
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 3 cut(s) 263, 419, 571
Bsp1286I GDGCHC 1 cut(s) 526
Bsp1407I TGTACA 1 cut(s) 328
Bsp143I GATC 3 cut(s) 357, 398, 540
Bsp19I CCATGG 2 cut(s) 198, 536
BspACI CCGC 2 cut(s) 428, 493
BspLI GGNNCC 3 cut(s) 48, 204, 426
BspMAI CTGCAG 1 cut(s) 435
BspPI GGATC 2 cut(s) 406, 548
BspQI GCTCTTC 1 cut(s) 163
BsrGI TGTACA 1 cut(s) 328
BsrI ACTGG 2 cut(s) 47, 132
BssECI CCNNGG 4 cut(s) 198, 245, 420, 536
BssMI GATC 3 cut(s) 357, 398, 540
BssT1I CCWWGG 2 cut(s) 198, 536
Bst2UI CCWGG 2 cut(s) 246, 421
Bst6I CTCTTC 1 cut(s) 163
BstAUI TGTACA 1 cut(s) 328
BstC8I GCNNGC 1 cut(s) 380
BstDSI CCRYGG 2 cut(s) 198, 536
BstKTI GATC 3 cut(s) 360, 401, 543
BstMAI GTCTC 1 cut(s) 340
BstMBI GATC 3 cut(s) 357, 398, 540
BstNI CCWGG 2 cut(s) 246, 421
BstSCI CCNGG 3 cut(s) 244, 265, 419
BstSFI CTRYAG 1 cut(s) 431
BstV1I GCAGC 1 cut(s) 417
BstX2I RGATCY 2 cut(s) 357, 540
BstYI RGATCY 2 cut(s) 357, 540
BtgI CCRYGG 2 cut(s) 198, 536
BtsIMutI CAGTG 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 380
CseI GACGC 1 cut(s) 357
Csp6I GTAC 2 cut(s) 329, 490
CviAII CATG 7 cut(s) 199, 293, 352, 363, 371, 402, 537
CviJI RGCY 3 cut(s) 156, 203, 427
CviKI_1 RGCY 3 cut(s) 156, 203, 427
CviQI GTAC 2 cut(s) 329, 490
DpnI GATC 3 cut(s) 359, 400, 542
DpnII GATC 3 cut(s) 357, 398, 540
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Eco130I CCWWGG 2 cut(s) 198, 536
EcoRI GAATTC 1 cut(s) 449
EcoRII CCWGG 2 cut(s) 244, 419
EcoT14I CCWWGG 2 cut(s) 198, 536
ErhI CCWWGG 2 cut(s) 198, 536
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 7 cut(s) 202, 296, 355, 366, 374, 405, 540
FaqI GGGAC 3 cut(s) 263, 419, 571
FatI CATG 7 cut(s) 198, 292, 351, 362, 370, 401, 536
Fnu4HI GCNGC 2 cut(s) 428, 431
Fsp4HI GCNGC 2 cut(s) 428, 431
GluI GCNGC 2 cut(s) 428, 431
GsuI CTGGAG 2 cut(s) 186, 510
HapII CCGG 1 cut(s) 266
HgaI GACGC 1 cut(s) 357
Hin1II CATG 7 cut(s) 202, 296, 355, 366, 374, 405, 540
HincII GTYRAC 2 cut(s) 258, 588
HindII GTYRAC 2 cut(s) 258, 588
HinfI GANTC 5 cut(s) 82, 194, 283, 367, 551
HpaI GTTAAC 1 cut(s) 258
HpaII CCGG 1 cut(s) 266
HphI GGTGA 1 cut(s) 201
Hpy166II GTNNAC 2 cut(s) 258, 588
Hpy188I TCNGA 3 cut(s) 87, 238, 339
Hpy188III TCNNGA 3 cut(s) 165, 182, 527
Hpy8I GTNNAC 2 cut(s) 258, 588
HpyAV CCTTC 3 cut(s) 140, 146, 235
HpyCH4IV ACGT 1 cut(s) 254
HpyCH4V TGCA 2 cut(s) 433, 583
HpySE526I ACGT 1 cut(s) 254
Hsp92II CATG 7 cut(s) 202, 296, 355, 366, 374, 405, 540
KspAI GTTAAC 1 cut(s) 258
Kzo9I GATC 3 cut(s) 357, 398, 540
LguI GCTCTTC 1 cut(s) 163
LmnI GCTCC 3 cut(s) 208, 424, 529
Lsp1109I GCAGC 1 cut(s) 417
MaeII ACGT 1 cut(s) 254
MaeIII GTNAC 2 cut(s) 38, 443
MalI GATC 3 cut(s) 359, 400, 542
MboI GATC 3 cut(s) 357, 398, 540
MboII GAAGA 5 cut(s) 100, 150, 153, 220, 367
MfeI CAATTG 1 cut(s) 409
MflI RGATCY 2 cut(s) 357, 540
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 3 cut(s) 409, 436, 449
MlyI GAGTC 2 cut(s) 277, 361
MmeI TCCRAC 1 cut(s) 494
MnlI CCTC 5 cut(s) 19, 136, 217, 222, 510
MseI TTAA 2 cut(s) 171, 257
MspA1I CMGCKG 1 cut(s) 430
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 3 cut(s) 246, 267, 421
MunI CAATTG 1 cut(s) 409
MvaI CCWGG 2 cut(s) 246, 421
NciI CCSGG 1 cut(s) 267
NcoI CCATGG 2 cut(s) 198, 536
NdeII GATC 3 cut(s) 357, 398, 540
NlaIII CATG 7 cut(s) 202, 296, 355, 366, 374, 405, 540
NlaIV GGNNCC 3 cut(s) 48, 204, 426
PciSI GCTCTTC 1 cut(s) 163
PfeI GAWTC 3 cut(s) 82, 194, 551
PkrI GCNGC 2 cut(s) 429, 432
PleI GAGTC 2 cut(s) 277, 361
PpsI GAGTC 2 cut(s) 277, 361
Psp6I CCWGG 2 cut(s) 244, 419
PspGI CCWGG 2 cut(s) 244, 419
PspN4I GGNNCC 3 cut(s) 48, 204, 426
PstI CTGCAG 1 cut(s) 435
PsuI RGATCY 2 cut(s) 357, 540
RsaI GTAC 2 cut(s) 330, 491
RsaNI GTAC 2 cut(s) 329, 490
SapI GCTCTTC 1 cut(s) 163
SaqAI TTAA 2 cut(s) 171, 257
SatI GCNGC 2 cut(s) 428, 431
Sau3AI GATC 3 cut(s) 357, 398, 540
SchI GAGTC 2 cut(s) 277, 361
ScrFI CCNGG 3 cut(s) 246, 267, 421
SduI GDGCHC 1 cut(s) 526
SetI ASST 5 cut(s) 214, 257, 445, 481, 521
SfcI CTRYAG 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 343
SmoI CTYRAG 1 cut(s) 343
Sse9I AATT 3 cut(s) 409, 436, 449
SsiI CCGC 2 cut(s) 428, 493
StyD4I CCNGG 3 cut(s) 244, 265, 419
StyI CCWWGG 2 cut(s) 198, 536
TaiI ACGT 1 cut(s) 257
TasI AATT 3 cut(s) 409, 436, 449
TatI WGTACW 1 cut(s) 328
TauI GCSGC 1 cut(s) 430
TfiI GAWTC 3 cut(s) 82, 194, 551
Tru1I TTAA 2 cut(s) 171, 257
Tru9I TTAA 2 cut(s) 171, 257
TscAI CASTG 1 cut(s) 139
TseI GCWGC 1 cut(s) 430
TspDTI ATGAA 4 cut(s) 95, 207, 368, 487
TspRI CASTG 1 cut(s) 139
XapI RAATTY 2 cut(s) 436, 449
XcmI CCANNNNNNNNNTGG 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.