MD09G1134400.v1.1

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006758
Physical Location & Seq
Forward (+)
10328377 .. 10336071
7695 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1134400.v1.1.491

Sequence Viewer

Length: 2721 bp
ATGGCGGGTCGAGGTGGAGGTAGGGACCGGTTTCCGGAGAGTCGCGGTGGGCACGGGCTGCGGCGGTTGGACTCCAAAGGTGGGAAAGCTCCGCCTTCCAGGAACCTATGGGTTGGTAACCTTTCTCATAGTACCGTGGAGGAAGATCTCGTTAATCCCTTTCTGCAGTTTGGAGAGCTAGAAAGCGTAGCCTTCCATCCCGGCCGGAGTTATGCCTTTCTTAAGTTCAAGAGGGAAGATGATGCAATCGCTGCCATGGATTCCCTCCAGGATCTTCCTGTTGCAGGCAACCCACTGAGGATCGAGTTTACCAAGGCGGATAAATCATCAGCACCATCGCGTGAAGAAGATTACTCCCAACGTCGAGATGAACAACGTACAGCTCCAAGAGGATCACCTTTTTATCAAATGGAGTTTAGAGCACGCCAGGATAGTCCTGAGCAATTTTATCAGGAAAAATCCAATATGAGTGATAAGAATGCAGAGCCTAGTGCTGTTTTATGGATAGGTTTTCCTGCTTTATTGAAGGTGGATGAAATGATATTAAGGAAAGCTTTCTCACCGTTTGGTGAGATTGAGAAGATCACTGCATTCCCCGGTCGTACTTATGCTTTTGTTCGATTTAGGAGTGAAGTGTCAGCGTGCAGAGCGAAAGAAGCTCTTCAGGGAAAATTATTTGGAAATCCCCGGGTGCATATTTGCTTTGCAAAGAGTGAAACAGGCTCAGCTAATAGTGGAAGGAATTCAATGAGTGTCCCTCCATCCCCTCATTTTCAGGTGAATGGTCGCTCAGGATCATCTGAGAATTTTCGAGAGGACACGAAATTTGGGAGCTTCACAGGTAATCCCAGCATCAGATCTCCTCATTATTTTCCAGATTTTGATGCTGTTGATTCTGATCCCTATGGTTTTAAGCGGAAGGGGAATTTATGGAGAGGTGAAAACAATATGTTTGAACAGAGGAGATACAAGGAAATGAGGTCTGAACTAGGACTATCAGAAGATATGTATGATCCTCGGGGTAGTCCTAAAAGAGAGAAATATTCTCATTTGAATGATTATTCACAGAGATTTCCTCAGACAAGTCAACCTTATGAAGAGCCATGGGACTTGCCAGAAGATATTCATTTATTTCATGGGGCCAAGAAATTGAAGACAGAGTCTTTTCTTTCGAACAAAGAGTTTCCAGAGTATCCCCAGTCTGACTACAACCCAGAAAGACATGGCTTTTCTAGGTCATACTCTGATTTTCCCCAAGCTGACAGTTCCAATAGAAACTTTGAGGGCGGGCCATTTGGTTACAACCAGATCCCTGATCGACCAATGAGTTTACCTCCAGCTCTAGAAGAAAGGGGTGACCGCTGGAAAGAATCTTATGATGATTTTCAGGTGAGCTCGGGTTCTCAGCTAGCAAATCCTGTTGACAGGAGAAGGTTCACTCCTGAACGCGATCGGCCTTCTTTCAATGTGTGGAAATGGGAAGGGACTATAGCAAAGGGAGGAACTCCTGTCTGTAATGCTCGTTGCTTTCCTGTGGGCAAACTCCTTGACTTCACATTGCCTGAATTTTTGGATTGTACTGCAAGGACTGGTCTAGACATGCTTTCAAAGCATTACTATCAAGCAGCAAGTGCTTGGGTTGTGTTCTTTGCCCCACAAAGTGATGCTGATATTGGATACTACAATGAATTTATGCATTATCTTGGGGAGAAGCAGCGAGCAGCCGTTGCTAAGTTAGATGACAAGAATACCTTGTTTCTTGTGCCCCCGTCAGACTTCTCAGAGAAAGTGCTGAAGGTACCAGGGAAACTCAGCATCTCCGGTGTTGTTTTGAGGTTAGAAAATCCTAGTTCCAATTTTGGATCGGTACACCAACAACCCGAAAGAAAAGACACGAGATTATTGTCATTGCCTGGGGATACATCATATACAAAGCCATCAACAGCTTCAGAATCTATTCATTCATTTACATCAGCTCCACCGGTGTCATTTTCAGGTTCAGCTTATGGTGTTGGAAATGGATCTGATTCTTACAACGAGAGCAGGCACGAGTATCCACATCATATGGAAAGCCCTGCATTGGGGCGAAACTGGTCTTCACACAAGCCGCAAAACTCAATTATGGATACTAGAAATAAATCAACCCAAATGCCCAATAGTTCCATTGATTCTATTTTTCAGGAGCATAGGATCATGCAAAGGGAAGCACAACAGTCGAGCATTCCAGGTGGAATTTCTCGTATTCGAAACAGCCATTCGTCACAGCAGGAAATCCAATCTCCTGGCTTTTTATCTGTGCCGTCTGAATCCCTGCAACCTGAGCAACTTGCACAATTGGCATCATCTCTTGGGCAGCAGCAGAGGCAGTCTGCGAGAACTCCAAATCCAGCTACCGGAGAAGATTTTAGACAAAGAAACACAATGAATGAGTCTGATAACATTCCAAGGACATCCCAGACATTTGGTATGCAGAATAATGAGGTGAATTCACAGCCATCAACATCTCAGTTTGGTCAAGTCCAACAGTTGCAGCAGCGGCAACAGCAGGTATCAACTGTGTCTGCAGCGCCTCACACTGTCCAAACAGCGGTTCAGGGAAACCAACAACTGCAAAGCACGAGTACAAATGAAGCAGTAGAAACAGATCCACAGAAACGTCTGCAAGCGACATTACAGTTGGCAGCAGCTCTTCTTCAGCAAATCCAAGGGAAAGGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0000785 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009553 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0019219 GO:0019222 GO:0022414 GO:0031047 GO:0031048 GO:0031123 GO:0031124 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0034641 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 SPOC

Protein Analysis

907

Amino Acids

101.86

Weight (kDa)

6.9

Isoelectric Point (pI)

65.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 36 - 99 2.3e-08 RNA recognition motif
RRM_1 PF00076 177 - 230 7.6e-09 RNA recognition motif
SPOC PF07744 491 - 605 2.2e-23 SPOC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2536
Acc65I GGTACC 1 cut(s) 1798
AccB1I GGYRCC 1 cut(s) 1798
AccII CGCG 3 cut(s) 45, 340, 1449
AccIII TCCGGA 1 cut(s) 34
AcoI YGGCCR 1 cut(s) 202
AcsI RAATTY 8 cut(s) 742, 805, 824, 925, 1565, 1688, 2232, 2485
AcuI CTGAAG 4 cut(s) 647, 1814, 1932, 2678
AdeI CACNNNGTG 1 cut(s) 1661
AfaI GTAC 7 cut(s) 133, 379, 604, 1579, 1800, 1869, 2623
AfiI CCNNNNNNNGG 7 cut(s) 34, 81, 284, 2080, 2081, 2393, 2587
AflII CTTAAG 1 cut(s) 221
AgeI ACCGGT 2 cut(s) 27, 1981
AgsI TTSAA 8 cut(s) 229, 526, 747, 956, 1054, 1153, 1465, 1608
AjnI CCWGG 7 cut(s) 98, 267, 426, 1801, 1912, 2224, 2281
AjuI GAANNNNNNNTTGG 2 cut(s) 2574, 2606
AloI GAACNNNNNNTCC 2 cut(s) 2368, 2400
Alw21I GWGCWC 2 cut(s) 424, 1397
Ama87I CYCGRG 3 cut(s) 687, 1017, 1396
Aor13HI TCCGGA 1 cut(s) 34
AoxI GGCC 4 cut(s) 202, 1140, 1289, 1454
ApoI RAATTY 8 cut(s) 742, 805, 824, 925, 1565, 1688, 2232, 2485
ArsI GACNNNNNNTTYG 2 cut(s) 809, 841
AsiGI ACCGGT 2 cut(s) 27, 1981
Asp700I GAANNNNTTC 5 cut(s) 554, 660, 742, 1122, 1956
Asp718I GGTACC 1 cut(s) 1798
AspLEI GCGC 1 cut(s) 2569
AspS9I GGNCC 3 cut(s) 25, 1140, 1289
AsuC2I CCSGG 4 cut(s) 201, 597, 688, 689
AsuHPI GGTGA 8 cut(s) 387, 552, 581, 790, 950, 1367, 1402, 2494
AsuII TTCGAA 2 cut(s) 1172, 2245
AsuNHI GCTAGC 1 cut(s) 1408
AvaI CYCGRG 3 cut(s) 687, 1017, 1396
AvaII GGWCC 1 cut(s) 25
BaeGI GKGCMC 2 cut(s) 54, 1767
BanI GGYRCC 1 cut(s) 1798
BanII GRGCYC 1 cut(s) 1397
BauI CACGAG 3 cut(s) 1894, 2048, 2617
BbsI GAAGAC 2 cut(s) 1160, 2088
Bbv12I GWGCWC 2 cut(s) 424, 1397
BccI CCATC 5 cut(s) 204, 343, 769, 1945, 2503
BceAI ACGGC 2 cut(s) 1709, 2284
BcgI CGANNNNNNTGC 2 cut(s) 2196, 2230
BciT130I CCWGG 7 cut(s) 100, 269, 428, 1803, 1914, 2226, 2283
BciVI GTATCC 5 cut(s) 1203, 1670, 1912, 2064, 2119
BcnI CCSGG 4 cut(s) 201, 597, 688, 689
BfaI CTAG 9 cut(s) 179, 489, 989, 1233, 1343, 1409, 1595, 1848, 2130
BfmI CTRYAG 3 cut(s) 164, 1488, 2562
BfoI RGCGCY 1 cut(s) 2570
BfrI CTTAAG 1 cut(s) 221
BfuAI ACCTGC 1 cut(s) 2536
BfuI GTATCC 5 cut(s) 1203, 1670, 1912, 2064, 2119
BglII AGATCT 2 cut(s) 145, 857
BlpI GCTNAGC 1 cut(s) 724
Bme18I GGWCC 1 cut(s) 25
BmeT110I CYCGRG 3 cut(s) 687, 1017, 1396
BmgT120I GGNCC 3 cut(s) 25, 1140, 1289
BmiI GGNNCC 4 cut(s) 26, 104, 1141, 1800
BmrI ACTGGG 1 cut(s) 1192
BmsI GCATC 6 cut(s) 232, 861, 874, 1654, 1824, 2348
BmtI GCTAGC 1 cut(s) 1412
BmuI ACTGGG 1 cut(s) 1192
BpiI GAAGAC 2 cut(s) 1160, 2088
BplI GAGNNNNNCTC 6 cut(s) 742, 774, 1060, 1092, 1318, 1350
BpmI CTGGAG 2 cut(s) 251, 1320
Bpu10I CCTNAGC 3 cut(s) 438, 790, 2319
Bpu1102I GCTNAGC 1 cut(s) 724
Bpu14I TTCGAA 2 cut(s) 1172, 2245
BpuMI CCSGG 4 cut(s) 201, 597, 688, 689
BsaBI GATNNNNATC 1 cut(s) 897
BsaWI WCCGGW 5 cut(s) 27, 34, 1820, 1981, 2393
BsaXI ACNNNNNCTCC 2 cut(s) 1960, 1990
Bsc4I CCNNNNNNNGG 7 cut(s) 34, 81, 284, 2080, 2081, 2393, 2587
Bse118I RCCGGY 2 cut(s) 27, 1981
Bse1I ACTGG 3 cut(s) 1198, 1594, 2096
Bse3DI GCAATG 2 cut(s) 1556, 1907
Bse8I GATNNNNATC 1 cut(s) 897
BseAI TCCGGA 1 cut(s) 34
BseBI CCWGG 7 cut(s) 100, 269, 428, 1803, 1914, 2226, 2283
BseGI GGATG 4 cut(s) 196, 538, 761, 2450
BseJI GATNNNNATC 1 cut(s) 897
BseLI CCNNNNNNNGG 7 cut(s) 34, 81, 284, 2080, 2081, 2393, 2587
BseMI GCAATG 2 cut(s) 1556, 1907
BseNI ACTGG 3 cut(s) 1198, 1594, 2096
BseRI GAGGAG 2 cut(s) 852, 976
BseSI GKGCMC 2 cut(s) 54, 1767
BseX3I CGGCCG 1 cut(s) 202
BseYI CCCAGC 1 cut(s) 848
BsgI GTGCAG 1 cut(s) 664
Bsh1236I CGCG 3 cut(s) 45, 340, 1449
Bsh1285I CGRYCG 3 cut(s) 205, 601, 1453
BshFI GGCC 4 cut(s) 204, 1142, 1291, 1456
BshNI GGYRCC 1 cut(s) 1798
BshTI ACCGGT 2 cut(s) 27, 1981
BsiEI CGRYCG 3 cut(s) 205, 601, 1453
BsiHKAI GWGCWC 2 cut(s) 424, 1397
BsiHKCI CYCGRG 3 cut(s) 687, 1017, 1396
BsiSI CCGG 9 cut(s) 28, 35, 201, 205, 597, 688, 1821, 1982, 2394
BslFI GGGAC 4 cut(s) 38, 740, 1121, 1498
BslI CCNNNNNNNGG 7 cut(s) 34, 81, 284, 2080, 2081, 2393, 2587
BsmFI GGGAC 4 cut(s) 38, 740, 1121, 1498
BsmI GAATGC 3 cut(s) 484, 590, 2220
BsnI GGCC 4 cut(s) 204, 1142, 1291, 1456
BsoBI CYCGRG 3 cut(s) 687, 1017, 1396
Bsp119I TTCGAA 2 cut(s) 1172, 2245
Bsp1286I GDGCHC 4 cut(s) 54, 424, 1397, 1767
Bsp13I TCCGGA 1 cut(s) 34
Bsp1720I GCTNAGC 1 cut(s) 724
Bsp19I CCATGG 2 cut(s) 255, 1103
BspANI GGCC 4 cut(s) 204, 1142, 1291, 1456
BspEI TCCGGA 1 cut(s) 34
BspFNI CGCG 3 cut(s) 45, 340, 1449
BspLI GGNNCC 4 cut(s) 26, 104, 1141, 1800
BspMAI CTGCAG 2 cut(s) 168, 2566
BspMI ACCTGC 1 cut(s) 2536
BspOI GCTAGC 1 cut(s) 1412
BspQI GCTCTTC 3 cut(s) 666, 1092, 2694
BspT104I TTCGAA 2 cut(s) 1172, 2245
BspT107I GGYRCC 1 cut(s) 1798
BspTI CTTAAG 1 cut(s) 221
BsrDI GCAATG 2 cut(s) 1556, 1907
BsrFI RCCGGY 2 cut(s) 27, 1981
BsrI ACTGG 3 cut(s) 1198, 1594, 2096
BssAI RCCGGY 2 cut(s) 27, 1981
BssSI CACGAG 3 cut(s) 1894, 2048, 2617
BssT1I CCWWGG 5 cut(s) 255, 312, 1103, 2444, 2704
Bst2BI CACGAG 3 cut(s) 1894, 2048, 2617
Bst2UI CCWGG 7 cut(s) 100, 269, 428, 1803, 1914, 2226, 2283
Bst4CI ACNGT 8 cut(s) 136, 564, 1265, 2214, 2526, 2557, 2578, 2676
Bst6I CTCTTC 3 cut(s) 666, 1092, 2694
BstAFI CTTAAG 1 cut(s) 221
BstAPI GCANNNNNTGC 4 cut(s) 58, 251, 1631, 1727
BstBI TTCGAA 2 cut(s) 1172, 2245
BstC8I GCNNGC 8 cut(s) 286, 424, 643, 1289, 1410, 1719, 2045, 2664
BstDSI CCRYGG 3 cut(s) 135, 255, 1103
BstEII GGTNACC 2 cut(s) 116, 1355
BstENI CCTNNNNNAGG 1 cut(s) 282
BstF5I GGATG 4 cut(s) 196, 538, 761, 2450
BstFNI CGCG 3 cut(s) 45, 340, 1449
BstH2I RGCGCY 1 cut(s) 2570
BstHHI GCGC 1 cut(s) 2569
BstMCI CGRYCG 3 cut(s) 205, 601, 1453
BstNI CCWGG 7 cut(s) 100, 269, 428, 1803, 1914, 2226, 2283
BstNSI RCATGY 1 cut(s) 1603
BstPI GGTNACC 2 cut(s) 116, 1355
BstSFI CTRYAG 3 cut(s) 164, 1488, 2562
BstSLI GKGCMC 2 cut(s) 54, 1767
BstUI CGCG 3 cut(s) 45, 340, 1449
BstV2I GAAGAC 2 cut(s) 1160, 2088
BstX2I RGATCY 6 cut(s) 145, 271, 857, 1308, 2021, 2644
BstXI CCANNNNNNTGG 2 cut(s) 2282, 2462
BstYI RGATCY 6 cut(s) 145, 271, 857, 1308, 2021, 2644
BstZI CGGCCG 1 cut(s) 202
BsuI GTATCC 5 cut(s) 1203, 1670, 1912, 2064, 2119
BsuRI GGCC 4 cut(s) 204, 1142, 1291, 1456
BtgI CCRYGG 3 cut(s) 135, 255, 1103
BtgZI GCGATG 1 cut(s) 321
BtsCI GGATG 4 cut(s) 196, 538, 761, 2450
BtsI GCAGTG 1 cut(s) 585
BtsIMutI CAGTG 3 cut(s) 293, 585, 2574
BveI ACCTGC 1 cut(s) 2536
Cac8I GCNNGC 8 cut(s) 286, 424, 643, 1289, 1410, 1719, 2045, 2664
CfoI GCGC 1 cut(s) 2569
Cfr10I RCCGGY 2 cut(s) 27, 1981
Cfr13I GGNCC 3 cut(s) 25, 1140, 1289
Cfr9I CCCGGG 1 cut(s) 687
Csp6I GTAC 7 cut(s) 132, 378, 603, 1578, 1799, 1868, 2622
CspAI ACCGGT 2 cut(s) 27, 1981
CviAII CATG 6 cut(s) 256, 1104, 1136, 1223, 1600, 2194
CviQI GTAC 7 cut(s) 132, 378, 603, 1578, 1799, 1868, 2622
DraIII CACNNNGTG 1 cut(s) 1661
EaeI YGGCCR 1 cut(s) 202
EagI CGGCCG 1 cut(s) 202
Eam1104I CTCTTC 3 cut(s) 666, 1092, 2694
EarI CTCTTC 3 cut(s) 666, 1092, 2694
EciI GGCGGA 2 cut(s) 81, 332
Ecl136II GAGCTC 1 cut(s) 1395
EclXI CGGCCG 1 cut(s) 202
Eco130I CCWWGG 5 cut(s) 255, 312, 1103, 2444, 2704
Eco24I GRGCYC 1 cut(s) 1397
Eco47I GGWCC 1 cut(s) 25
Eco52I CGGCCG 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 1395
Eco57I CTGAAG 4 cut(s) 647, 1814, 1932, 2678
Eco88I CYCGRG 3 cut(s) 687, 1017, 1396
Eco91I GGTNACC 2 cut(s) 116, 1355
EcoICRI GAGCTC 1 cut(s) 1395
EcoNI CCTNNNNNAGG 1 cut(s) 282
EcoO65I GGTNACC 2 cut(s) 116, 1355
EcoRI GAATTC 2 cut(s) 742, 2485
EcoRII CCWGG 7 cut(s) 98, 267, 426, 1801, 1912, 2224, 2281
EcoT14I CCWWGG 5 cut(s) 255, 312, 1103, 2444, 2704
EcoT22I ATGCAT 1 cut(s) 1698
EcoT38I GRGCYC 1 cut(s) 1397
ErhI CCWWGG 5 cut(s) 255, 312, 1103, 2444, 2704
FaeI CATG 6 cut(s) 259, 1107, 1139, 1226, 1603, 2197
FalI AAGNNNNNCTT 8 cut(s) 538, 570, 645, 677, 1075, 1107, 1736, 1768
FaqI GGGAC 4 cut(s) 38, 740, 1121, 1498
FatI CATG 6 cut(s) 255, 1103, 1135, 1222, 1599, 2193
FauI CCCGC 1 cut(s) 1280
FauNDI CATATG 1 cut(s) 2064
FokI GGATG 4 cut(s) 183, 545, 748, 2437
FriOI GRGCYC 1 cut(s) 1397
FspBI CTAG 9 cut(s) 179, 489, 989, 1233, 1343, 1409, 1595, 1848, 2130
GlaI GCGC 1 cut(s) 2568
GsaI CCCAGC 1 cut(s) 852
GsuI CTGGAG 2 cut(s) 251, 1320
HaeII RGCGCY 1 cut(s) 2570
HaeIII GGCC 4 cut(s) 204, 1142, 1291, 1456
HapII CCGG 9 cut(s) 28, 35, 201, 205, 597, 688, 1821, 1982, 2394
HhaI GCGC 1 cut(s) 2569
Hin1II CATG 6 cut(s) 259, 1107, 1139, 1226, 1603, 2197
Hin6I GCGC 1 cut(s) 2567
HinP1I GCGC 1 cut(s) 2567
HincII GTYRAC 2 cut(s) 1088, 1423
HindII GTYRAC 2 cut(s) 1088, 1423
HindIII AAGCTT 1 cut(s) 552
HpaII CCGG 9 cut(s) 28, 35, 201, 205, 597, 688, 1821, 1982, 2394
HphI GGTGA 8 cut(s) 387, 552, 581, 790, 950, 1367, 1402, 2494
Hpy166II GTNNAC 6 cut(s) 309, 1088, 1331, 1423, 1437, 1870
Hpy8I GTNNAC 6 cut(s) 309, 1088, 1331, 1423, 1437, 1870
Hpy99I CGWCG 1 cut(s) 366
HpyAV CCTTC 9 cut(s) 105, 202, 520, 732, 913, 1425, 1467, 1475, 1789
HpyCH4III ACNGT 8 cut(s) 136, 564, 1265, 2214, 2526, 2557, 2578, 2676
HpyCH4IV ACGT 3 cut(s) 361, 376, 2656
HpySE526I ACGT 3 cut(s) 361, 376, 2656
Hsp92II CATG 6 cut(s) 259, 1107, 1139, 1226, 1603, 2197
HspAI GCGC 1 cut(s) 2567
Kpn2I TCCGGA 1 cut(s) 34
KpnI GGTACC 1 cut(s) 1802
LguI GCTCTTC 3 cut(s) 666, 1092, 2694
LmnI GCTCC 5 cut(s) 94, 388, 831, 1981, 2182
LweI GCATC 6 cut(s) 232, 861, 874, 1654, 1824, 2348
MaeI CTAG 9 cut(s) 179, 489, 989, 1233, 1343, 1409, 1595, 1848, 2130
MaeII ACGT 3 cut(s) 361, 376, 2656
MaeIII GTNAC 4 cut(s) 116, 1298, 1355, 2259
MfeI CAATTG 1 cut(s) 2333
MflI RGATCY 6 cut(s) 145, 271, 857, 1308, 2021, 2644
MhlI GDGCHC 4 cut(s) 54, 424, 1397, 1767
MlyI GAGTC 4 cut(s) 49, 65, 1169, 2438
MmeI TCCRAC 3 cut(s) 48, 1993, 2545
Mph1103I ATGCAT 1 cut(s) 1698
MroI TCCGGA 1 cut(s) 34
MroXI GAANNNNTTC 5 cut(s) 554, 660, 742, 1122, 1956
MseI TTAA 4 cut(s) 153, 222, 545, 912
MslI CAYNNNNRTG 1 cut(s) 1053
MspA1I CMGCKG 3 cut(s) 1362, 2536, 2588
MspCI CTTAAG 1 cut(s) 221
MspI CCGG 9 cut(s) 28, 35, 201, 205, 597, 688, 1821, 1982, 2394
MunI CAATTG 1 cut(s) 2333
Mva1269I GAATGC 3 cut(s) 484, 590, 2220
MvaI CCWGG 7 cut(s) 100, 269, 428, 1803, 1914, 2226, 2283
MvnI CGCG 3 cut(s) 45, 340, 1449
NciI CCSGG 4 cut(s) 201, 597, 688, 689
NcoI CCATGG 2 cut(s) 255, 1103
NdeI CATATG 1 cut(s) 2064
NheI GCTAGC 1 cut(s) 1408
NlaIII CATG 6 cut(s) 259, 1107, 1139, 1226, 1603, 2197
NlaIV GGNNCC 4 cut(s) 26, 104, 1141, 1800
NmuCI GTSAC 2 cut(s) 1355, 2259
NsiI ATGCAT 1 cut(s) 1698
NspI RCATGY 1 cut(s) 1603
NspV TTCGAA 2 cut(s) 1172, 2245
PciSI GCTCTTC 3 cut(s) 666, 1092, 2694
PctI GAATGC 3 cut(s) 484, 590, 2220
PdmI GAANNNNTTC 5 cut(s) 554, 660, 742, 1122, 1956
PfeI GAWTC 7 cut(s) 260, 893, 1370, 1952, 2027, 2168, 2306
PflFI GACNNNGTC 1 cut(s) 1159
PfoI TCCNGGA 2 cut(s) 98, 267
PinAI ACCGGT 2 cut(s) 27, 1981
Ple19I CGATCG 1 cut(s) 1453
PleI GAGTC 4 cut(s) 48, 65, 1168, 2437
PpsI GAGTC 4 cut(s) 48, 65, 1168, 2437
Psp124BI GAGCTC 1 cut(s) 1397
Psp6I CCWGG 7 cut(s) 98, 267, 426, 1801, 1912, 2224, 2281
PspEI GGTNACC 2 cut(s) 116, 1355
PspFI CCCAGC 1 cut(s) 848
PspGI CCWGG 7 cut(s) 98, 267, 426, 1801, 1912, 2224, 2281
PspN4I GGNNCC 4 cut(s) 26, 104, 1141, 1800
PspPI GGNCC 3 cut(s) 25, 1140, 1289
PstI CTGCAG 2 cut(s) 168, 2566
PsuI RGATCY 6 cut(s) 145, 271, 857, 1308, 2021, 2644
PsyI GACNNNGTC 1 cut(s) 1159
PvuI CGATCG 1 cut(s) 1453
RsaI GTAC 7 cut(s) 133, 379, 604, 1579, 1800, 1869, 2623
RsaNI GTAC 7 cut(s) 132, 378, 603, 1578, 1799, 1868, 2622
RseI CAYNNNNRTG 1 cut(s) 1053
SacI GAGCTC 1 cut(s) 1397
SapI GCTCTTC 3 cut(s) 666, 1092, 2694
SaqAI TTAA 4 cut(s) 153, 222, 545, 912
Sau96I GGNCC 3 cut(s) 25, 1140, 1289
SchI GAGTC 4 cut(s) 49, 65, 1169, 2438
SduI GDGCHC 4 cut(s) 54, 424, 1397, 1767
SfaNI GCATC 6 cut(s) 232, 861, 874, 1654, 1824, 2348
SfcI CTRYAG 3 cut(s) 164, 1488, 2562
SfuI TTCGAA 2 cut(s) 1172, 2245
SgrAI CRCCGGYG 1 cut(s) 1981
SinI GGWCC 1 cut(s) 25
SmaI CCCGGG 1 cut(s) 689
SmiMI CAYNNNNRTG 1 cut(s) 1053
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
SspI AATATT 1 cut(s) 1043
SspMI CTAG 9 cut(s) 179, 489, 989, 1233, 1343, 1409, 1595, 1848, 2130
SstI GAGCTC 1 cut(s) 1397
StyI CCWWGG 5 cut(s) 255, 312, 1103, 2444, 2704
TaaI ACNGT 8 cut(s) 136, 564, 1265, 2214, 2526, 2557, 2578, 2676
TaiI ACGT 3 cut(s) 364, 379, 2659
TaqI TCGA 9 cut(s) 10, 303, 364, 619, 811, 1172, 1318, 2216, 2245
TatI WGTACW 2 cut(s) 1577, 2621
TauI GCSGC 3 cut(s) 64, 2110, 2539
TfiI GAWTC 7 cut(s) 260, 893, 1370, 1952, 2027, 2168, 2306
Tru1I TTAA 4 cut(s) 153, 222, 545, 912
Tru9I TTAA 4 cut(s) 153, 222, 545, 912
TscAI CASTG 3 cut(s) 300, 592, 2581
TseFI GTSAC 2 cut(s) 1355, 2259
Tsp45I GTSAC 2 cut(s) 1355, 2259
TspMI CCCGGG 1 cut(s) 687
TspRI CASTG 3 cut(s) 300, 592, 2581
Tth111I GACNNNGTC 1 cut(s) 1159
Vha464I CTTAAG 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 25
XagI CCTNNNNNAGG 1 cut(s) 282
XapI RAATTY 8 cut(s) 742, 805, 824, 925, 1565, 1688, 2232, 2485
XbaI TCTAGA 2 cut(s) 1342, 1594
XceI RCATGY 1 cut(s) 1603
XmaI CCCGGG 1 cut(s) 687
XmnI GAANNNNTTC 5 cut(s) 554, 660, 742, 1122, 1956
XspI CTAG 9 cut(s) 179, 489, 989, 1233, 1343, 1409, 1595, 1848, 2130
Zsp2I ATGCAT 1 cut(s) 1698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.