MD17G1123300.v1.1

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006758
Physical Location & Seq
Forward (+)
10709480 .. 10711478
1999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1123300.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGAATCTACTTCTAGCTCTAGGAGAAACTCCTGTCAGTCATGCTCGTTGCTTTCCTGTGGGCAAACTCCTTGACATCACTTTGCCTGAATTTTTGGATTGTACTGCAAGGACTGGTCTAGACATGCTTTCAAAGCATTACCATCAAGCAGCAAGTGCTTGGGTTGTGTTCTTTGCCCCTCAAAGTGATGCTGATTTTGGATGCTACAATGAATTTATGCATTACCTTGGGGAAAAGCAGCGAGCAGCCGTTGCGAAGTTAGATGACAAGAATACCTTGTTTCTTGTGCCCCCATCGGACTTCTCGGAGAAAGTGCTGAAGGTACCAGGGAAACTCAGCATCTCCGGTGTTGTTTTGAGGTTAGAGAATCCTAGTTCAAATTTTCGATCCCCTCATCAAGAACCTGAAAGAAAAGATATGAGATTATTGTCATTGCCATGGGATCATATACAAAGCCATCAACACCTTCAGGATCTATTCATTCATATACATCAGCTCCACCGGTGTCATTTTCAGGTTCAGCTTATGGTGTTGGAAATGGATCCGATTCTTACAATGAGAACAGGCATGAGTATCCGCATCATATGGAAAGCCCTGCATCGGGGCCAAACTGGTCTTCACTCCACACAATACTCAATTACAGATACTAGAAATAGACCAACCCAATTGCCCAATGGTGTCGCTGATTCTAATTTTCAGGAGCATAGGGTCATGCAAAGGGAAGCACAACAGTTGAGCATTTCAGGTGGAATTTCTCATATTCGAAACCACAATTCGTCGCAGCAGGAAATCCAATCCTCAGGTTCTTTATCTATGCTATCTGCTGCCCCGCAACCAGAGCAACTTGAACAATTGCCATCATCTCTTATTGCACAGCAGCGGAGGCAGCCTAGGAGAACTCCAAATCCATCTACTGGAGAAGATTTAAGACGGAGAAACACAATGAATGAGTCTGATAACATTCCAAGGACATCCCATACATTTGGTATGCTGAATAATCAGGTGACGTTGCAGCTGTCAACGTCTCAGTTTGGTCAAGTTCAACAGTTGCAGCAGCGGCAACAGCATGTATCAACTGTGTCTGCAACGCCTCACACAGTCCAAACAGCACTTCAGGGAAACCAACAGCTACAAAGTACTAGTACAAATAAAGCAATAGAAACAGATCCACAGAAACGCCTGCAAGCAACATTACAGTTGGCTGCAGCTCTTCAACAAAGCCAAGGGAAATTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0000785 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009553 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0019219 GO:0019222 GO:0022414 GO:0031047 GO:0031048 GO:0031123 GO:0031124 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0034641 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 SPOC

Protein Analysis

413

Amino Acids

46.45

Weight (kDa)

9.08

Isoelectric Point (pI)

60.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOC PF07744 5 - 114 2.6e-19 SPOC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 322
AccB1I GGYRCC 1 cut(s) 322
AccB7I CCANNNNNTGG 1 cut(s) 914
AciI CCGC 4 cut(s) 577, 830, 880, 1057
AclWI GGATC 6 cut(s) 381, 450, 480, 536, 549, 1160
AcsI RAATTY 4 cut(s) 89, 212, 379, 750
AcuI CTGAAG 3 cut(s) 338, 452, 1097
AfaI GTAC 4 cut(s) 103, 324, 1138, 1144
AfiI CCNNNNNNNGG 3 cut(s) 600, 601, 914
AgeI ACCGGT 1 cut(s) 501
AgsI TTSAA 5 cut(s) 132, 378, 848, 1043, 1214
AhlI ACTAGT 1 cut(s) 1139
AjnI CCWGG 1 cut(s) 325
AjuI GAANNNNNNNTTGG 4 cut(s) 600, 632, 1095, 1127
AloI GAACNNNNNNTCC 2 cut(s) 889, 921
AluBI AGCT 6 cut(s) 17, 496, 523, 1015, 1129, 1208
AluI AGCT 6 cut(s) 17, 496, 523, 1015, 1129, 1208
Alw26I GTCTC 1 cut(s) 1029
AlwI GGATC 6 cut(s) 381, 450, 480, 536, 549, 1160
AoxI GGCC 1 cut(s) 604
ApoI RAATTY 4 cut(s) 89, 212, 379, 750
AsiGI ACCGGT 1 cut(s) 501
Asp718I GGTACC 1 cut(s) 322
AspA2I CCTAGG 1 cut(s) 890
AspS9I GGNCC 1 cut(s) 604
AsuHPI GGTGA 1 cut(s) 1015
AsuII TTCGAA 1 cut(s) 763
AvrII CCTAGG 1 cut(s) 890
AxyI CCTNAGG 1 cut(s) 799
BaeGI GKGCMC 1 cut(s) 291
BamHI GGATCC 1 cut(s) 541
BanI GGYRCC 1 cut(s) 322
BbsI GAAGAC 1 cut(s) 608
BccI CCATC 5 cut(s) 150, 301, 465, 865, 916
BceAI ACGGC 1 cut(s) 233
BciT130I CCWGG 1 cut(s) 327
BciVI GTATCC 1 cut(s) 584
BcoDI GTCTC 1 cut(s) 1029
BcuI ACTAGT 1 cut(s) 1139
BfaI CTAG 7 cut(s) 14, 20, 119, 372, 648, 891, 1140
BfmI CTRYAG 1 cut(s) 1203
BfuI GTATCC 1 cut(s) 584
BlnI CCTAGG 1 cut(s) 890
BmcAI AGTACT 1 cut(s) 1138
Bme1390I CCNGG 1 cut(s) 327
BmgT120I GGNCC 1 cut(s) 604
BmiI GGNNCC 3 cut(s) 324, 543, 605
BmrFI CCNGG 1 cut(s) 327
BmsI GCATC 5 cut(s) 178, 191, 348, 588, 607
BpiI GAAGAC 1 cut(s) 608
BpmI CTGGAG 1 cut(s) 936
Bpu14I TTCGAA 1 cut(s) 763
BsaJI CCNNGG 6 cut(s) 226, 326, 437, 890, 965, 1222
BsaWI WCCGGW 2 cut(s) 344, 501
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bsc4I CCNNNNNNNGG 3 cut(s) 600, 601, 914
Bse118I RCCGGY 1 cut(s) 501
Bse1I ACTGG 3 cut(s) 118, 616, 919
Bse21I CCTNAGG 1 cut(s) 799
Bse3DI GCAATG 1 cut(s) 431
BseBI CCWGG 1 cut(s) 327
BseDI CCNNGG 6 cut(s) 226, 326, 437, 890, 965, 1222
BseGI GGATG 2 cut(s) 206, 971
BseLI CCNNNNNNNGG 3 cut(s) 600, 601, 914
BseMI GCAATG 1 cut(s) 431
BseMII CTCAG 3 cut(s) 349, 813, 1040
BseNI ACTGG 3 cut(s) 118, 616, 919
BseSI GKGCMC 1 cut(s) 291
BshFI GGCC 1 cut(s) 606
BshNI GGYRCC 1 cut(s) 322
BshTI ACCGGT 1 cut(s) 501
BsiSI CCGG 2 cut(s) 345, 502
BslI CCNNNNNNNGG 3 cut(s) 600, 601, 914
BsmAI GTCTC 1 cut(s) 1029
BsmBI CGTCTC 1 cut(s) 1029
BsnI GGCC 1 cut(s) 606
Bsp119I TTCGAA 1 cut(s) 763
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 5 cut(s) 386, 442, 472, 541, 1165
Bsp19I CCATGG 1 cut(s) 437
BspACI CCGC 4 cut(s) 577, 830, 880, 1057
BspANI GGCC 1 cut(s) 606
BspCNI CTCAG 3 cut(s) 348, 812, 1039
BspLI GGNNCC 3 cut(s) 324, 543, 605
BspMAI CTGCAG 1 cut(s) 1207
BspPI GGATC 6 cut(s) 381, 450, 480, 536, 549, 1160
BspQI GCTCTTC 1 cut(s) 1215
BspT104I TTCGAA 1 cut(s) 763
BspT107I GGYRCC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 431
BsrFI RCCGGY 1 cut(s) 501
BsrI ACTGG 3 cut(s) 118, 616, 919
BssAI RCCGGY 1 cut(s) 501
BssECI CCNNGG 6 cut(s) 226, 326, 437, 890, 965, 1222
BssMI GATC 5 cut(s) 386, 442, 472, 541, 1165
BssT1I CCWWGG 5 cut(s) 226, 437, 890, 965, 1222
Bst2UI CCWGG 1 cut(s) 327
Bst4CI ACNGT 5 cut(s) 732, 1047, 1078, 1099, 1197
Bst6I CTCTTC 1 cut(s) 1215
BstAPI GCANNNNNTGC 2 cut(s) 155, 251
BstBI TTCGAA 1 cut(s) 763
BstC8I GCNNGC 3 cut(s) 243, 1181, 1185
BstDEI CTNAG 3 cut(s) 335, 799, 1026
BstDSI CCRYGG 1 cut(s) 437
BstF5I GGATG 2 cut(s) 206, 971
BstKTI GATC 5 cut(s) 389, 445, 475, 544, 1168
BstMAI GTCTC 1 cut(s) 1029
BstMBI GATC 5 cut(s) 386, 442, 472, 541, 1165
BstMWI GCNNNNNNNGC 8 cut(s) 133, 155, 251, 838, 883, 886, 1057, 1063
BstNI CCWGG 1 cut(s) 327
BstNSI RCATGY 2 cut(s) 127, 1070
BstSCI CCNGG 1 cut(s) 325
BstSFI CTRYAG 1 cut(s) 1203
BstSLI GKGCMC 1 cut(s) 291
BstV2I GAAGAC 1 cut(s) 608
BstX2I RGATCY 3 cut(s) 472, 541, 1165
BstXI CCANNNNNNTGG 1 cut(s) 983
BstYI RGATCY 3 cut(s) 472, 541, 1165
Bsu36I CCTNAGG 1 cut(s) 799
BsuI GTATCC 1 cut(s) 584
BsuRI GGCC 1 cut(s) 606
BtgI CCRYGG 1 cut(s) 437
BtsCI GGATG 2 cut(s) 206, 971
Cac8I GCNNGC 3 cut(s) 243, 1181, 1185
Cfr10I RCCGGY 1 cut(s) 501
Cfr13I GGNCC 1 cut(s) 604
Csp6I GTAC 4 cut(s) 102, 323, 1137, 1143
CspAI ACCGGT 1 cut(s) 501
CviAII CATG 6 cut(s) 41, 124, 438, 568, 712, 1067
CviQI GTAC 4 cut(s) 102, 323, 1137, 1143
DdeI CTNAG 3 cut(s) 335, 799, 1026
DpnI GATC 5 cut(s) 388, 444, 474, 543, 1167
DpnII GATC 5 cut(s) 386, 442, 472, 541, 1165
Eam1104I CTCTTC 1 cut(s) 1215
EarI CTCTTC 1 cut(s) 1215
Eco130I CCWWGG 5 cut(s) 226, 437, 890, 965, 1222
Eco57I CTGAAG 3 cut(s) 338, 452, 1097
Eco81I CCTNAGG 1 cut(s) 799
EcoRII CCWGG 1 cut(s) 325
EcoT14I CCWWGG 5 cut(s) 226, 437, 890, 965, 1222
EcoT22I ATGCAT 1 cut(s) 222
ErhI CCWWGG 5 cut(s) 226, 437, 890, 965, 1222
Esp3I CGTCTC 1 cut(s) 1029
FaeI CATG 6 cut(s) 44, 127, 441, 571, 715, 1070
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FatI CATG 6 cut(s) 40, 123, 437, 567, 711, 1066
FauI CCCGC 1 cut(s) 837
FauNDI CATATG 1 cut(s) 584
FokI GGATG 2 cut(s) 213, 958
FspBI CTAG 7 cut(s) 14, 20, 119, 372, 648, 891, 1140
GsuI CTGGAG 1 cut(s) 936
HaeIII GGCC 1 cut(s) 606
HapII CCGG 2 cut(s) 345, 502
Hin1II CATG 6 cut(s) 44, 127, 441, 571, 715, 1070
HincII GTYRAC 1 cut(s) 1020
HindII GTYRAC 1 cut(s) 1020
HinfI GANTC 5 cut(s) 4, 367, 547, 686, 950
HpaII CCGG 2 cut(s) 345, 502
HphI GGTGA 1 cut(s) 1015
Hpy166II GTNNAC 1 cut(s) 1020
Hpy188I TCNGA 4 cut(s) 298, 307, 546, 955
Hpy188III TCNNGA 4 cut(s) 119, 398, 470, 698
Hpy8I GTNNAC 1 cut(s) 1020
Hpy99I CGWCG 1 cut(s) 781
HpyAV CCTTC 2 cut(s) 313, 476
HpyCH4III ACNGT 5 cut(s) 732, 1047, 1078, 1099, 1197
HpyCH4IV ACGT 2 cut(s) 1007, 1022
HpyF10VI GCNNNNNNNGC 8 cut(s) 133, 155, 251, 838, 883, 886, 1057, 1063
HpyF3I CTNAG 3 cut(s) 335, 799, 1026
HpySE526I ACGT 2 cut(s) 1007, 1022
Hsp92II CATG 6 cut(s) 44, 127, 441, 571, 715, 1070
KpnI GGTACC 1 cut(s) 326
Kzo9I GATC 5 cut(s) 386, 442, 472, 541, 1165
LguI GCTCTTC 1 cut(s) 1215
LmnI GCTCC 2 cut(s) 501, 700
LweI GCATC 5 cut(s) 178, 191, 348, 588, 607
MaeI CTAG 7 cut(s) 14, 20, 119, 372, 648, 891, 1140
MaeII ACGT 2 cut(s) 1007, 1022
MaeIII GTNAC 1 cut(s) 1003
MalI GATC 5 cut(s) 388, 444, 474, 543, 1167
MboI GATC 5 cut(s) 386, 442, 472, 541, 1165
MboII GAAGA 3 cut(s) 608, 932, 1202
MfeI CAATTG 2 cut(s) 665, 851
MflI RGATCY 3 cut(s) 472, 541, 1165
MhlI GDGCHC 1 cut(s) 291
MlyI GAGTC 1 cut(s) 959
MmeI TCCRAC 1 cut(s) 513
MnlI CCTC 6 cut(s) 189, 351, 402, 808, 876, 1101
Mph1103I ATGCAT 1 cut(s) 222
MseI TTAA 2 cut(s) 926, 1232
MslI CAYNNNNRTG 1 cut(s) 436
MspA1I CMGCKG 3 cut(s) 880, 1015, 1057
MspI CCGG 2 cut(s) 345, 502
MspR9I CCNGG 1 cut(s) 327
MunI CAATTG 2 cut(s) 665, 851
MvaI CCWGG 1 cut(s) 327
MwoI GCNNNNNNNGC 8 cut(s) 133, 155, 251, 838, 883, 886, 1057, 1063
NcoI CCATGG 1 cut(s) 437
NdeI CATATG 1 cut(s) 584
NdeII GATC 5 cut(s) 386, 442, 472, 541, 1165
NlaIII CATG 6 cut(s) 44, 127, 441, 571, 715, 1070
NlaIV GGNNCC 3 cut(s) 324, 543, 605
NmuCI GTSAC 1 cut(s) 1003
NsiI ATGCAT 1 cut(s) 222
NspI RCATGY 2 cut(s) 127, 1070
NspV TTCGAA 1 cut(s) 763
PciSI GCTCTTC 1 cut(s) 1215
PfeI GAWTC 4 cut(s) 4, 367, 547, 686
PflMI CCANNNNNTGG 1 cut(s) 914
PinAI ACCGGT 1 cut(s) 501
PleI GAGTC 1 cut(s) 958
PpsI GAGTC 1 cut(s) 958
Psp6I CCWGG 1 cut(s) 325
PspGI CCWGG 1 cut(s) 325
PspN4I GGNNCC 3 cut(s) 324, 543, 605
PspPI GGNCC 1 cut(s) 604
PstI CTGCAG 1 cut(s) 1207
PsuI RGATCY 3 cut(s) 472, 541, 1165
PvuII CAGCTG 1 cut(s) 1015
RsaI GTAC 4 cut(s) 103, 324, 1138, 1144
RsaNI GTAC 4 cut(s) 102, 323, 1137, 1143
RseI CAYNNNNRTG 1 cut(s) 436
SapI GCTCTTC 1 cut(s) 1215
SaqAI TTAA 2 cut(s) 926, 1232
Sau3AI GATC 5 cut(s) 386, 442, 472, 541, 1165
Sau96I GGNCC 1 cut(s) 604
ScaI AGTACT 1 cut(s) 1138
SchI GAGTC 1 cut(s) 959
ScrFI CCNGG 1 cut(s) 327
SduI GDGCHC 1 cut(s) 291
SfaNI GCATC 5 cut(s) 178, 191, 348, 588, 607
SfcI CTRYAG 1 cut(s) 1203
SfuI TTCGAA 1 cut(s) 763
SgrAI CRCCGGYG 1 cut(s) 501
SmiMI CAYNNNNRTG 1 cut(s) 436
SpeI ACTAGT 1 cut(s) 1139
SsiI CCGC 4 cut(s) 577, 830, 880, 1057
SspMI CTAG 7 cut(s) 14, 20, 119, 372, 648, 891, 1140
StyD4I CCNGG 1 cut(s) 325
StyI CCWWGG 5 cut(s) 226, 437, 890, 965, 1222
TaaI ACNGT 5 cut(s) 732, 1047, 1078, 1099, 1197
TaiI ACGT 2 cut(s) 1010, 1025
TaqI TCGA 2 cut(s) 385, 763
TatI WGTACW 3 cut(s) 101, 1136, 1142
TauI GCSGC 1 cut(s) 1060
TfiI GAWTC 4 cut(s) 4, 367, 547, 686
Tru1I TTAA 2 cut(s) 926, 1232
Tru9I TTAA 2 cut(s) 926, 1232
TseFI GTSAC 1 cut(s) 1003
Tsp45I GTSAC 1 cut(s) 1003
TspDTI ATGAA 5 cut(s) 17, 225, 469, 473, 959
TspGWI ACGGA 1 cut(s) 946
Van91I CCANNNNNTGG 1 cut(s) 914
XapI RAATTY 4 cut(s) 89, 212, 379, 750
XbaI TCTAGA 1 cut(s) 118
XceI RCATGY 2 cut(s) 127, 1070
XcmI CCANNNNNNNNNTGG 1 cut(s) 671
XmaJI CCTAGG 1 cut(s) 890
XspI CTAG 7 cut(s) 14, 20, 119, 372, 648, 891, 1140
ZrmI AGTACT 1 cut(s) 1138
Zsp2I ATGCAT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.