MD09G1150800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011396
Physical Location & Seq
Forward (+)
11915316 .. 11918547
3232 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1150800.v1.1.491

Sequence Viewer

Length: 576 bp
ATGGGCCTCATCGATGGGGGGAACCCCCATTCATCCGGAAAGAAGAGAGAGAGAGAGATGATACAGAGGAGTGGTGGTGGAACAGCCATGGTTGTTCGTCGGAGAGCTTCATGGAGGACTCCCATGTGCTTGGCTTCCAACGTCAACACAGAGGAGCTACGTGCCCATCTCGGCCAGCTCCACTCTGAGGCTGAGACCGCCAGAGCCAAAGCAAACAATGCAAGATTGAGGCTTTTGCGTCTGTCAGAGGCAGCCGAGAAGCTGAAACGGCAAGCAGCAATTAATGTCCCAACCGGGAAGGAAGACGATGCAAGGGAGCAGCTCTTGCAGAAGAAAAAGGTCATGGAAGAAGTCAAAGCACCGCATAGAGCGATTTCTCTGAAAGAAAGGCAGCTAATCGGGAATGTTTCTTTGGATCTTGGACTTGTCAGAGAAGATGCTTTTAGTCCTGTTCGAATTGTATCACCAACAACGGAAGTTGCTGAAGATTTGAAGGAGAGCAAAGAATTTGTCCCAAATGATCTGAATAAAGAAACGCCGCTTCCTAAAGACAACCAAGAAAAGCTTACCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

21.29

Weight (kDa)

9.44

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011396)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06510
fragaria_vesca FvH4_6g38360 FvH4_6g38360
malus_domestica MD09G1150800.v1.1 MD17G1138200.v1.1
prunus_persica Prupe.3G174200_v2.0.a1
pyrus_communis pycom09g07060 pycom17g13140
rosa_chinensis RchiOBHm_Chr2g0151671
rosa_laevigata RLG00000020567
rosa_multiflora Rmu_sc0005294.1_g000050
rosa_roxburghii Rroxscaffold_2G00096270
rosa_rugosa Rorug02G0427700 Rorug02G0427800 Rorug02G0427900
rosa_samantha Rh2AG488600 Rh2BG501300 Rh2CG475100 Rh2DG512400
rosa_wichuraiana Rw2G040070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 35
AciI CCGC 3 cut(s) 198, 362, 539
AclWI GGATC 1 cut(s) 423
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 1 cut(s) 506
AcuI CTGAAG 1 cut(s) 504
AfiI CCNNNNNNNGG 1 cut(s) 187
AgsI TTSAA 1 cut(s) 493
AjuI GAANNNNNNNTTGG 2 cut(s) 395, 427
AluBI AGCT 7 cut(s) 107, 157, 178, 262, 322, 394, 565
AluI AGCT 7 cut(s) 107, 157, 178, 262, 322, 394, 565
Alw26I GTCTC 1 cut(s) 188
AlwI GGATC 1 cut(s) 423
Aor13HI TCCGGA 1 cut(s) 35
AoxI GGCC 2 cut(s) 4, 172
ApeKI GCWGC 4 cut(s) 251, 275, 319, 391
ApoI RAATTY 1 cut(s) 506
ArsI GACNNNNNNTTYG 2 cut(s) 495, 527
AseI ATTAAT 1 cut(s) 282
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 295
AsuHPI GGTGA 1 cut(s) 456
AsuII TTCGAA 1 cut(s) 454
BaeGI GKGCMC 1 cut(s) 166
BbsI GAAGAC 1 cut(s) 309
BbvI GCAGC 4 cut(s) 263, 287, 331, 403
BccI CCATC 2 cut(s) 8, 174
BceAI ACGGC 1 cut(s) 284
BcnI CCSGG 1 cut(s) 295
BcoDI GTCTC 1 cut(s) 188
BisI GCNGC 5 cut(s) 252, 276, 320, 392, 539
BlsI GCNGC 5 cut(s) 253, 277, 321, 393, 540
Bme1390I CCNGG 1 cut(s) 295
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 23
BmrFI CCNGG 1 cut(s) 295
BmsI GCATC 2 cut(s) 298, 427
BpiI GAAGAC 1 cut(s) 309
Bpu14I TTCGAA 1 cut(s) 454
BpuMI CCSGG 1 cut(s) 295
Bsa29I ATCGAT 1 cut(s) 12
BsaAI YACGTR 1 cut(s) 161
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 35
BsaXI ACNNNNNCTCC 2 cut(s) 61, 91
Bsc4I CCNNNNNNNGG 1 cut(s) 187
BseAI TCCGGA 1 cut(s) 35
BseCI ATCGAT 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 32
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMII CTCAG 2 cut(s) 177, 183
BseRI GAGGAG 2 cut(s) 82, 167
BseSI GKGCMC 1 cut(s) 166
BseXI GCAGC 4 cut(s) 263, 287, 331, 403
BshFI GGCC 2 cut(s) 6, 174
BshVI ATCGAT 1 cut(s) 12
BsiSI CCGG 2 cut(s) 36, 294
BslFI GGGAC 2 cut(s) 272, 497
BslI CCNNNNNNNGG 1 cut(s) 187
BsmAI GTCTC 1 cut(s) 188
BsmFI GGGAC 2 cut(s) 272, 497
BsnI GGCC 2 cut(s) 6, 174
Bso31I GGTCTC 1 cut(s) 188
Bsp119I TTCGAA 1 cut(s) 454
Bsp1286I GDGCHC 1 cut(s) 166
Bsp13I TCCGGA 1 cut(s) 35
Bsp143I GATC 2 cut(s) 415, 520
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 3 cut(s) 198, 362, 539
BspANI GGCC 2 cut(s) 6, 174
BspCNI CTCAG 2 cut(s) 178, 184
BspDI ATCGAT 1 cut(s) 12
BspEI TCCGGA 1 cut(s) 35
BspLI GGNNCC 1 cut(s) 23
BspPI GGATC 1 cut(s) 423
BspT104I TTCGAA 1 cut(s) 454
BspTNI GGTCTC 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 2 cut(s) 415, 520
BssT1I CCWWGG 1 cut(s) 87
Bst6I CTCTTC 1 cut(s) 38
BstAPI GCANNNNNTGC 2 cut(s) 218, 325
BstBAI YACGTR 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 454
BstC8I GCNNGC 2 cut(s) 176, 273
BstDEI CTNAG 2 cut(s) 186, 192
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 32
BstKTI GATC 2 cut(s) 418, 523
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 2 cut(s) 415, 520
BstMWI GCNNNNNNNGC 4 cut(s) 197, 218, 268, 325
BstSCI CCNGG 1 cut(s) 293
BstSLI GKGCMC 1 cut(s) 166
BstV1I GCAGC 4 cut(s) 263, 287, 331, 403
BstV2I GAAGAC 1 cut(s) 309
BstX2I RGATCY 1 cut(s) 415
BstXI CCANNNNNNTGG 1 cut(s) 130
BstYI RGATCY 1 cut(s) 415
Bsu15I ATCGAT 1 cut(s) 12
BsuRI GGCC 2 cut(s) 6, 174
BsuTUI ATCGAT 1 cut(s) 12
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 1 cut(s) 32
Cac8I GCNNGC 2 cut(s) 176, 273
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 12
CseI GACGC 1 cut(s) 227
CviAII CATG 4 cut(s) 88, 111, 124, 343
DdeI CTNAG 2 cut(s) 186, 192
DpnI GATC 2 cut(s) 417, 522
DpnII GATC 2 cut(s) 415, 520
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 87
Eco31I GGTCTC 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 504
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 4 cut(s) 91, 114, 127, 346
FaiI YATR 5 cut(s) 89, 112, 125, 344, 366
FalI AAGNNNNNCTT 1 cut(s) 549
FaqI GGGAC 2 cut(s) 272, 497
FatI CATG 4 cut(s) 87, 110, 123, 342
Fnu4HI GCNGC 5 cut(s) 252, 276, 320, 392, 539
FokI GGATG 1 cut(s) 19
Fsp4HI GCNGC 5 cut(s) 252, 276, 320, 392, 539
GluI GCNGC 5 cut(s) 252, 276, 320, 392, 539
HaeIII GGCC 2 cut(s) 6, 174
HapII CCGG 2 cut(s) 36, 294
HgaI GACGC 1 cut(s) 227
Hin1II CATG 4 cut(s) 91, 114, 127, 346
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 1 cut(s) 118
HpaII CCGG 2 cut(s) 36, 294
HphI GGTGA 1 cut(s) 456
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 6 cut(s) 102, 187, 247, 381, 431, 525
Hpy188III TCNNGA 2 cut(s) 36, 400
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 2 cut(s) 292, 487
HpyCH4IV ACGT 2 cut(s) 141, 160
HpyCH4V TGCA 3 cut(s) 221, 311, 328
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 218, 268, 325
HpyF3I CTNAG 2 cut(s) 186, 192
HpySE526I ACGT 2 cut(s) 141, 160
Hsp92II CATG 4 cut(s) 91, 114, 127, 346
Kpn2I TCCGGA 1 cut(s) 35
Kzo9I GATC 2 cut(s) 415, 520
LmnI GCTCC 3 cut(s) 154, 183, 316
LpnPI CCDG 5 cut(s) 49, 188, 214, 307, 462
Lsp1109I GCAGC 4 cut(s) 263, 287, 331, 403
LweI GCATC 2 cut(s) 298, 427
MaeII ACGT 2 cut(s) 141, 160
MalI GATC 2 cut(s) 417, 522
MboI GATC 2 cut(s) 415, 520
MboII GAAGA 6 cut(s) 55, 314, 343, 359, 446, 497
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 1 cut(s) 166
MluCI AATT 3 cut(s) 279, 456, 506
MlyI GAGTC 1 cut(s) 112
MmeI TCCRAC 2 cut(s) 80, 162
MnlI CCTC 7 cut(s) 17, 60, 108, 145, 181, 222, 241
MroI TCCGGA 1 cut(s) 35
MseI TTAA 1 cut(s) 282
MspI CCGG 2 cut(s) 36, 294
MspR9I CCNGG 1 cut(s) 295
MwoI GCNNNNNNNGC 4 cut(s) 197, 218, 268, 325
NciI CCSGG 1 cut(s) 295
NcoI CCATGG 1 cut(s) 87
NdeII GATC 2 cut(s) 415, 520
NlaIII CATG 4 cut(s) 91, 114, 127, 346
NlaIV GGNNCC 1 cut(s) 23
NmeAIII GCCGAG 2 cut(s) 150, 280
NspV TTCGAA 1 cut(s) 454
PkrI GCNGC 5 cut(s) 253, 277, 321, 393, 540
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
Ppu21I YACGTR 1 cut(s) 161
PshBI ATTAAT 1 cut(s) 282
PspN4I GGNNCC 1 cut(s) 23
PspPI GGNCC 1 cut(s) 4
PsuI RGATCY 1 cut(s) 415
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 5 cut(s) 252, 276, 320, 392, 539
Sau3AI GATC 2 cut(s) 415, 520
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 112
ScrFI CCNGG 1 cut(s) 295
SduI GDGCHC 1 cut(s) 166
SfaNI GCATC 2 cut(s) 298, 427
SfuI TTCGAA 1 cut(s) 454
Sse9I AATT 3 cut(s) 279, 456, 506
SsiI CCGC 3 cut(s) 198, 362, 539
StyD4I CCNGG 1 cut(s) 293
StyI CCWWGG 1 cut(s) 87
TaiI ACGT 2 cut(s) 144, 163
TaqI TCGA 2 cut(s) 12, 454
TasI AATT 3 cut(s) 279, 456, 506
TauI GCSGC 1 cut(s) 541
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TseI GCWGC 4 cut(s) 251, 275, 319, 391
TspDTI ATGAA 2 cut(s) 21, 99
TspGWI ACGGA 1 cut(s) 488
VspI ATTAAT 1 cut(s) 282
XapI RAATTY 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.