MD09G1162800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0018091
Physical Location & Seq
Forward (+)
13223982 .. 13226358
2377 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1162800.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGGCTCAGGCGGAATTTAATCACTTTACCTATCTCAGTCCTGAGCAGCTGCGCAAGCTTGCTCGTATACTTTACTACCAAGAAGCTGAGGCCATTAAGAATATCCAGTTCGAATACGAAGCCAAACTCGCGAAATACCTCCAGGATTCCAAAGCTTGTTATGAATCGGCGATAAGGCTTTTGGATAGTGCTACTACGTTGGAGCCTTGGTTTGATCACGGCGACAAGGTTCGGGCTTCTATTGCTCACGACATCTACGGTTATATTGAAAACGCTGCCAACCATGCAATCCAGTTCGTGAAAAACTTTTATGTGCGAATGGATTACGTAAACAAGATTAAAAAGCATGTCAATGAACTCAGTGGTAAACTGGATGATCAAGTGACCAATATGGAAGAGCTGGCAAAAGAAGCTCACAAATTCAGGAACGACTCGTTGGAGATCGCCAGGGGTAGTCAAAGTCCCGCATCACGCCAATACTCCAGATGGATCAAGGAAACCGGCAAGACGTTTGAAGAACTAGTGCAGAAGTATCAGACAGAGCTTGGATACGGAAGACGTCGTTTTGCAGACCTTGAAGATTTTGAAAAGATAAAGGTGTACGGCACAATCATATCTAAATCCGGACGGGCAAATATTGATGTAACTGCGTATTCAAAGATTTTTGGGGCTGCTGGCATAGCTGTTCTTATTCTCGCTGCAGGAATTTTAGTATGGGACATATATTCAGCAGACAATCCACTTCAAACCGCGGCGCGTGACGCTGTAACGACTGCAGCAGCAATTGGTGGTGCGATGATAGGAGAAGTTGTTGGAGTTGCCCTCGCATCCATGGTCACTGGCAATGCTTTGTTAGTACTCATGGCGGGAATAACAGCCGGCATCGCCGGCGCTTTTCTTATCGGAGAATTCGCCATCTGGTTGATTAATGTGATTTTTGGGTCTGGAGGCAGTGCCCCATTGAGCACGATGGGGCATCGATGTTACGTCACACAGTTGCCCGAGGGCTCAGTTCTGGCACGCCAAATTGCACATCAGCAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

349

Amino Acids

38.44

Weight (kDa)

6.47

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018091)

Species Orthologous Gene IDs
malus_domestica MD09G1162800.v1.1 MD10G1107800.v1.1 MD17G1147800.v1.1
pyrus_communis pycom09g08030
rosa_laevigata RLG00000019198
rosa_multiflora Rmu_sc0001516.1_g000062 Rmu_sc0001516.1_g000068
rosa_roxburghii Rroxscaffold_1G00006390
rosa_samantha Rh2AG462000 Rh2AG462300 Rh2CG449000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 562
Acc16I TGCGCA 1 cut(s) 53
AccI GTMKAC 1 cut(s) 67
AccII CGCG 3 cut(s) 131, 752, 757
AccIII TCCGGA 1 cut(s) 623
AciI CCGC 5 cut(s) 11, 465, 750, 752, 866
AclWI GGATC 1 cut(s) 497
AcsI RAATTY 4 cut(s) 14, 419, 705, 908
AcyI GRCGYC 1 cut(s) 559
AfaI GTAC 2 cut(s) 602, 858
AgsI TTSAA 6 cut(s) 269, 515, 578, 587, 657, 746
AhlI ACTAGT 1 cut(s) 520
AjnI CCWGG 2 cut(s) 141, 446
AjuI GAANNNNNNNTTGG 2 cut(s) 419, 451
AluBI AGCT 8 cut(s) 49, 58, 86, 155, 400, 413, 544, 683
AluI AGCT 8 cut(s) 49, 58, 86, 155, 400, 413, 544, 683
Alw21I GWGCWC 1 cut(s) 968
AlwI GGATC 1 cut(s) 497
Ama87I CYCGRG 1 cut(s) 1001
Aor13HI TCCGGA 1 cut(s) 623
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 7 cut(s) 46, 49, 275, 671, 698, 776, 779
ApoI RAATTY 4 cut(s) 14, 419, 705, 908
AseI ATTAAT 1 cut(s) 927
AspLEI GCGC 3 cut(s) 54, 757, 893
AsuII TTCGAA 1 cut(s) 111
AvaI CYCGRG 1 cut(s) 1001
BaeGI GKGCMC 1 cut(s) 958
BanII GRGCYC 1 cut(s) 1010
BbsI GAAGAC 1 cut(s) 562
Bbv12I GWGCWC 1 cut(s) 968
BbvCI CCTCAGC 1 cut(s) 87
BbvI GCAGC 7 cut(s) 36, 58, 262, 658, 685, 788, 791
BccI CCATC 3 cut(s) 480, 923, 964
BceAI ACGGC 2 cut(s) 235, 619
BciT130I CCWGG 2 cut(s) 143, 448
BciVI GTATCC 1 cut(s) 542
BclI TGATCA 2 cut(s) 214, 376
BcuI ACTAGT 1 cut(s) 520
BfaI CTAG 1 cut(s) 521
BfmI CTRYAG 2 cut(s) 699, 774
BfoI RGCGCY 1 cut(s) 894
BfuI GTATCC 1 cut(s) 542
BisI GCNGC 8 cut(s) 47, 50, 276, 672, 699, 753, 777, 780
BlsI GCNGC 8 cut(s) 48, 51, 277, 673, 700, 754, 778, 781
BmcAI AGTACT 1 cut(s) 858
Bme1390I CCNGG 2 cut(s) 143, 448
BmeT110I CYCGRG 1 cut(s) 1001
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 2 cut(s) 143, 448
BmsI GCATC 4 cut(s) 476, 836, 891, 985
BpiI GAAGAC 1 cut(s) 562
BplI GAGNNNNNCTC 2 cut(s) 807, 839
BpmI CTGGAG 3 cut(s) 125, 466, 966
Bpu10I CCTNAGC 3 cut(s) 6, 42, 87
Bpu14I TTCGAA 1 cut(s) 111
Bsa29I ATCGAT 1 cut(s) 979
BsaAI YACGTR 1 cut(s) 328
BsaHI GRCGYC 1 cut(s) 559
BsaJI CCNNGG 5 cut(s) 206, 447, 750, 831, 1002
BsaWI WCCGGW 1 cut(s) 623
BsaXI ACNNNNNCTCC 4 cut(s) 194, 224, 795, 825
Bse118I RCCGGY 3 cut(s) 500, 878, 887
Bse1I ACTGG 4 cut(s) 106, 292, 375, 844
Bse3DI GCAATG 1 cut(s) 850
BseAI TCCGGA 1 cut(s) 623
BseBI CCWGG 2 cut(s) 143, 448
BseCI ATCGAT 1 cut(s) 979
BseDI CCNNGG 5 cut(s) 206, 447, 750, 831, 1002
BseGI GGATG 2 cut(s) 379, 827
BseMI GCAATG 1 cut(s) 850
BseMII CTCAG 6 cut(s) 20, 33, 49, 78, 373, 1023
BseNI ACTGG 4 cut(s) 106, 292, 375, 844
BseSI GKGCMC 1 cut(s) 958
BseXI GCAGC 7 cut(s) 36, 58, 262, 658, 685, 788, 791
BsgI GTGCAG 1 cut(s) 545
Bsh1236I CGCG 3 cut(s) 131, 752, 757
BshFI GGCC 1 cut(s) 92
BshVI ATCGAT 1 cut(s) 979
BsiHKAI GWGCWC 1 cut(s) 968
BsiHKCI CYCGRG 1 cut(s) 1001
BsiSI CCGG 4 cut(s) 501, 624, 879, 888
BslFI GGGAC 2 cut(s) 447, 731
BsmFI GGGAC 2 cut(s) 447, 731
BsnI GGCC 1 cut(s) 92
BsoBI CYCGRG 1 cut(s) 1001
Bsp119I TTCGAA 1 cut(s) 111
Bsp1286I GDGCHC 3 cut(s) 958, 968, 1010
Bsp13I TCCGGA 1 cut(s) 623
Bsp143I GATC 4 cut(s) 214, 376, 441, 489
Bsp19I CCATGG 1 cut(s) 831
Bsp68I TCGCGA 1 cut(s) 131
BspACI CCGC 5 cut(s) 11, 465, 750, 752, 866
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 6 cut(s) 19, 34, 48, 79, 372, 1022
BspDI ATCGAT 1 cut(s) 979
BspEI TCCGGA 1 cut(s) 623
BspFNI CGCG 3 cut(s) 131, 752, 757
BspLI GGNNCC 1 cut(s) 204
BspMAI CTGCAG 2 cut(s) 703, 778
BspPI GGATC 1 cut(s) 497
BspQI GCTCTTC 1 cut(s) 390
BspT104I TTCGAA 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 850
BsrFI RCCGGY 3 cut(s) 500, 878, 887
BsrI ACTGG 4 cut(s) 106, 292, 375, 844
BssAI RCCGGY 3 cut(s) 500, 878, 887
BssECI CCNNGG 5 cut(s) 206, 447, 750, 831, 1002
BssMI GATC 4 cut(s) 214, 376, 441, 489
BssNAI GTATAC 1 cut(s) 68
BssNI GRCGYC 1 cut(s) 559
BssT1I CCWWGG 2 cut(s) 206, 831
Bst1107I GTATAC 1 cut(s) 68
Bst2UI CCWGG 2 cut(s) 143, 448
Bst4CI ACNGT 2 cut(s) 260, 996
Bst6I CTCTTC 1 cut(s) 390
BstACI GRCGYC 1 cut(s) 559
BstBAI YACGTR 1 cut(s) 328
BstBI TTCGAA 1 cut(s) 111
BstC8I GCNNGC 7 cut(s) 56, 60, 402, 676, 880, 889, 1021
BstDEI CTNAG 6 cut(s) 6, 35, 42, 87, 359, 1009
BstDSI CCRYGG 2 cut(s) 750, 831
BstF5I GGATG 2 cut(s) 379, 827
BstFNI CGCG 3 cut(s) 131, 752, 757
BstH2I RGCGCY 1 cut(s) 894
BstHHI GCGC 3 cut(s) 54, 757, 893
BstKTI GATC 4 cut(s) 217, 379, 444, 492
BstMBI GATC 4 cut(s) 214, 376, 441, 489
BstMWI GCNNNNNNNGC 9 cut(s) 55, 128, 242, 284, 410, 680, 761, 884, 888
BstNI CCWGG 2 cut(s) 143, 448
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 2 cut(s) 141, 446
BstSFI CTRYAG 2 cut(s) 699, 774
BstSLI GKGCMC 1 cut(s) 958
BstSNI TACGTA 1 cut(s) 328
BstUI CGCG 3 cut(s) 131, 752, 757
BstV1I GCAGC 7 cut(s) 36, 58, 262, 658, 685, 788, 791
BstV2I GAAGAC 1 cut(s) 562
BstZ17I GTATAC 1 cut(s) 68
Bsu15I ATCGAT 1 cut(s) 979
BsuI GTATCC 1 cut(s) 542
BsuRI GGCC 1 cut(s) 92
BsuTUI ATCGAT 1 cut(s) 979
BtgI CCRYGG 2 cut(s) 750, 831
BtgZI GCGATG 2 cut(s) 809, 868
BtsCI GGATG 2 cut(s) 379, 827
BtsI GCAGTG 1 cut(s) 958
BtsIMutI CAGTG 3 cut(s) 367, 837, 958
BtuMI TCGCGA 1 cut(s) 131
Cac8I GCNNGC 7 cut(s) 56, 60, 402, 676, 880, 889, 1021
CfoI GCGC 3 cut(s) 54, 757, 893
Cfr10I RCCGGY 3 cut(s) 500, 878, 887
Cfr42I CCGCGG 1 cut(s) 753
ClaI ATCGAT 1 cut(s) 979
CseI GACGC 1 cut(s) 770
Csp6I GTAC 2 cut(s) 601, 857
CviAII CATG 4 cut(s) 284, 347, 832, 862
CviQI GTAC 2 cut(s) 601, 857
DdeI CTNAG 6 cut(s) 6, 35, 42, 87, 359, 1009
DpnI GATC 4 cut(s) 216, 378, 443, 491
DpnII GATC 4 cut(s) 214, 376, 441, 489
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
EciI GGCGGA 1 cut(s) 26
Eco105I TACGTA 1 cut(s) 328
Eco130I CCWWGG 2 cut(s) 206, 831
Eco24I GRGCYC 1 cut(s) 1010
Eco88I CYCGRG 1 cut(s) 1001
EcoRI GAATTC 1 cut(s) 908
EcoRII CCWGG 2 cut(s) 141, 446
EcoT14I CCWWGG 2 cut(s) 206, 831
EcoT38I GRGCYC 1 cut(s) 1010
ErhI CCWWGG 2 cut(s) 206, 831
FaeI CATG 4 cut(s) 287, 350, 835, 865
FaqI GGGAC 2 cut(s) 447, 731
FatI CATG 4 cut(s) 283, 346, 831, 861
FauI CCCGC 2 cut(s) 472, 859
FbaI TGATCA 2 cut(s) 214, 376
FblI GTMKAC 1 cut(s) 67
Fnu4HI GCNGC 8 cut(s) 47, 50, 276, 672, 699, 753, 777, 780
FokI GGATG 2 cut(s) 386, 814
FriOI GRGCYC 1 cut(s) 1010
Fsp4HI GCNGC 8 cut(s) 47, 50, 276, 672, 699, 753, 777, 780
FspBI CTAG 1 cut(s) 521
FspI TGCGCA 1 cut(s) 53
GlaI GCGC 3 cut(s) 53, 756, 892
GluI GCNGC 8 cut(s) 47, 50, 276, 672, 699, 753, 777, 780
GsuI CTGGAG 3 cut(s) 125, 466, 966
HaeII RGCGCY 1 cut(s) 894
HaeIII GGCC 1 cut(s) 92
HapII CCGG 4 cut(s) 501, 624, 879, 888
HgaI GACGC 1 cut(s) 770
HhaI GCGC 3 cut(s) 54, 757, 893
Hin1I GRCGYC 1 cut(s) 559
Hin1II CATG 4 cut(s) 287, 350, 835, 865
Hin6I GCGC 3 cut(s) 52, 755, 891
HinP1I GCGC 3 cut(s) 52, 755, 891
HindIII AAGCTT 2 cut(s) 56, 153
HinfI GANTC 3 cut(s) 146, 164, 431
HpaII CCGG 4 cut(s) 501, 624, 879, 888
Hpy166II GTNNAC 4 cut(s) 68, 331, 368, 601
Hpy188I TCNGA 2 cut(s) 537, 905
Hpy188III TCNNGA 8 cut(s) 41, 130, 248, 298, 424, 483, 624, 945
Hpy8I GTNNAC 4 cut(s) 68, 331, 368, 601
Hpy99I CGWCG 1 cut(s) 564
HpyCH4III ACNGT 2 cut(s) 260, 996
HpyCH4IV ACGT 5 cut(s) 197, 327, 509, 559, 987
HpyCH4V TGCA 6 cut(s) 287, 526, 569, 701, 776, 1031
HpyF10VI GCNNNNNNNGC 9 cut(s) 55, 128, 242, 284, 410, 680, 761, 884, 888
HpyF3I CTNAG 6 cut(s) 6, 35, 42, 87, 359, 1009
HpySE526I ACGT 5 cut(s) 197, 327, 509, 559, 987
Hsp92I GRCGYC 1 cut(s) 559
Hsp92II CATG 4 cut(s) 287, 350, 835, 865
HspAI GCGC 3 cut(s) 52, 755, 891
Kpn2I TCCGGA 1 cut(s) 623
KroI GCCGGC 2 cut(s) 878, 887
KroNI GCCGGC 2 cut(s) 880, 889
Ksp22I TGATCA 2 cut(s) 214, 376
KspI CCGCGG 1 cut(s) 753
Kzo9I GATC 4 cut(s) 214, 376, 441, 489
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 7 cut(s) 36, 58, 262, 658, 685, 788, 791
LweI GCATC 4 cut(s) 476, 836, 891, 985
MaeI CTAG 1 cut(s) 521
MaeII ACGT 5 cut(s) 197, 327, 509, 559, 987
MaeIII GTNAC 7 cut(s) 382, 643, 758, 766, 835, 983, 988
MalI GATC 4 cut(s) 216, 378, 443, 491
MboI GATC 4 cut(s) 214, 376, 441, 489
MboII GAAGA 4 cut(s) 407, 527, 567, 590
MfeI CAATTG 1 cut(s) 783
MhlI GDGCHC 3 cut(s) 958, 968, 1010
MluCI AATT 6 cut(s) 14, 419, 705, 783, 908, 1026
MlyI GAGTC 1 cut(s) 425
MmeI TCCRAC 3 cut(s) 180, 417, 793
MnlI CCTC 5 cut(s) 82, 149, 833, 941, 997
MreI CGCCGGCG 1 cut(s) 887
MroI TCCGGA 1 cut(s) 623
MroNI GCCGGC 2 cut(s) 878, 887
MseI TTAA 5 cut(s) 18, 96, 339, 927, 1045
MslI CAYNNNNRTG 1 cut(s) 351
MspA1I CMGCKG 2 cut(s) 49, 752
MspI CCGG 4 cut(s) 501, 624, 879, 888
MspR9I CCNGG 2 cut(s) 143, 448
MunI CAATTG 1 cut(s) 783
MvaI CCWGG 2 cut(s) 143, 448
MvnI CGCG 3 cut(s) 131, 752, 757
MwoI GCNNNNNNNGC 9 cut(s) 55, 128, 242, 284, 410, 680, 761, 884, 888
NaeI GCCGGC 2 cut(s) 880, 889
NcoI CCATGG 1 cut(s) 831
NdeII GATC 4 cut(s) 214, 376, 441, 489
NgoMIV GCCGGC 2 cut(s) 878, 887
NlaIII CATG 4 cut(s) 287, 350, 835, 865
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 4 cut(s) 382, 758, 835, 988
NruI TCGCGA 1 cut(s) 131
NsbI TGCGCA 1 cut(s) 53
NspI RCATGY 1 cut(s) 350
NspV TTCGAA 1 cut(s) 111
PciSI GCTCTTC 1 cut(s) 390
PdiI GCCGGC 2 cut(s) 880, 889
PfeI GAWTC 2 cut(s) 146, 164
PfoI TCCNGGA 1 cut(s) 141
PkrI GCNGC 8 cut(s) 48, 51, 277, 673, 700, 754, 778, 781
PleI GAGTC 1 cut(s) 425
PpsI GAGTC 1 cut(s) 425
Ppu21I YACGTR 1 cut(s) 328
PshBI ATTAAT 1 cut(s) 927
Psp6I CCWGG 2 cut(s) 141, 446
PspGI CCWGG 2 cut(s) 141, 446
PspN4I GGNNCC 1 cut(s) 204
PstI CTGCAG 2 cut(s) 703, 778
PvuII CAGCTG 1 cut(s) 49
RruI TCGCGA 1 cut(s) 131
RsaI GTAC 2 cut(s) 602, 858
RsaNI GTAC 2 cut(s) 601, 857
RseI CAYNNNNRTG 1 cut(s) 351
SacII CCGCGG 1 cut(s) 753
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 5 cut(s) 18, 96, 339, 927, 1045
SatI GCNGC 8 cut(s) 47, 50, 276, 672, 699, 753, 777, 780
Sau3AI GATC 4 cut(s) 214, 376, 441, 489
ScaI AGTACT 1 cut(s) 858
SchI GAGTC 1 cut(s) 425
ScrFI CCNGG 2 cut(s) 143, 448
SduI GDGCHC 3 cut(s) 958, 968, 1010
SfaNI GCATC 4 cut(s) 476, 836, 891, 985
SfcI CTRYAG 2 cut(s) 699, 774
Sfr303I CCGCGG 1 cut(s) 753
SfuI TTCGAA 1 cut(s) 111
SgrAI CRCCGGYG 1 cut(s) 887
SgrBI CCGCGG 1 cut(s) 753
SmiMI CAYNNNNRTG 1 cut(s) 351
SnaBI TACGTA 1 cut(s) 328
SpeI ACTAGT 1 cut(s) 520
Sse9I AATT 6 cut(s) 14, 419, 705, 783, 908, 1026
SsiI CCGC 5 cut(s) 11, 465, 750, 752, 866
SspI AATATT 1 cut(s) 637
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 2 cut(s) 141, 446
StyI CCWWGG 2 cut(s) 206, 831
TaaI ACNGT 2 cut(s) 260, 996
TaiI ACGT 5 cut(s) 200, 330, 512, 562, 990
TaqI TCGA 2 cut(s) 111, 979
TasI AATT 6 cut(s) 14, 419, 705, 783, 908, 1026
TatI WGTACW 1 cut(s) 856
TauI GCSGC 1 cut(s) 755
TfiI GAWTC 2 cut(s) 146, 164
Tru1I TTAA 5 cut(s) 18, 96, 339, 927, 1045
Tru9I TTAA 5 cut(s) 18, 96, 339, 927, 1045
TscAI CASTG 3 cut(s) 367, 844, 958
TseFI GTSAC 4 cut(s) 382, 758, 835, 988
TseI GCWGC 7 cut(s) 46, 49, 275, 671, 698, 776, 779
Tsp45I GTSAC 4 cut(s) 382, 758, 835, 988
TspDTI ATGAA 2 cut(s) 177, 369
TspGWI ACGGA 1 cut(s) 567
TspRI CASTG 3 cut(s) 367, 844, 958
VspI ATTAAT 1 cut(s) 927
XapI RAATTY 4 cut(s) 14, 419, 705, 908
XceI RCATGY 1 cut(s) 350
XmiI GTMKAC 1 cut(s) 67
XspI CTAG 1 cut(s) 521
ZraI GACGTC 1 cut(s) 560
ZrmI AGTACT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.