MD09G1171300.v1.1

ATPase 10, plasma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014890
Physical Location & Seq
Forward (+)
14479172 .. 14485598
6427 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1171300.v1.1.491

Sequence Viewer

Length: 2847 bp
ATGGATCTGGATAAACCATTGTTGGATCCTGAAAATTTCAACCGGGAGGGTATCGATTTGGAACGCTTACCATTGGGAGAAGTTTTCGAACAACTGAGGACATCACCACAAGGTCTTTCAACAGAAGATGCCGAAGTCAGATTGCAGATTTTTGGATTTAACAAGCTTGAAGAGAAAACGGAGAACAAATTTTTGAAGTTCCTTAGTTTTATGTGGAACCCCTTGTCATGGGTTATGGAAGCTGCAGCTGTAATGGCGCTTGTCCTCGCTAATGGTGGAGGTGAGGGTCCTGATTGGCAGGACTTTGTAGGGATTGTTATCCTGCTAATCATCAACTCAACGATTAGTTTCATAGAGGAGAATAATGCAGGGAATGCTGCATCAGCTCTAATGGATCGCTTAGCTCCAAAGACAAGGGTTCTTAGAGATGGGCGCTGGACAGAGCAAGATGCATCTATTTTAGTACCAGGTGACATAATTAGCATTAAGCTCGGGGACATAATTCCGGCTGATGCTCGTCTGCTTGAAGGAGATCCCCTGAAAGTTGACCAGTCAGCTCTTACAGGAGAGTCTCTAGCTGTCACCAAGAAGACAGGTGATGAAGTATTTTCTGGTTCAACGTGTAAGCATGGAGAGATTGAAGCTGTTGTGATCGCAACCGGAGTTAACTCCTTTTTCGGAAGGGCAGCACATTTGGTGGACTCCACTGAAGTTGTTGGACATTTCCAGCAGGTACTTACCGCCATTGGGAATTTCTGCATTTGCTCTATTGCTGTGGGAATGATTCTTGAAATCATTGTCATGTTCCCAGTACAGCACCGCTCTTACAGGGATGGAATCAACAACCTCCTTGTTCTCTTAATTGGAGGAATTCCGATCGCTATGCCAACGGTGTTATCTGTCACACTTGCCATTGGTTCTCATCGACTTTCTCAACAGGGTGCCATTACGAAAAGGATGACAGCAATTGAAGAGATGGCAGGAATGGATGTCCTCTGCAGTGACAAAACCGGAACTCTCACTCTGAATCGCCTCACTGTTGATAGAAACCTAGTCGAGGTTTTCAACAAAGATCTAGATAAAGACGCGGTTATCATGTTTGCAGCCAGAGCAGCTAGACTGGAGAACCAGGACGCTATTGATGCAGCCATCGTCAATATGCTTGCTGATCCGAAGGAGGCACGGGCGGGTATTACAGAAGTGCATTTTCTGCCCTTCAATCCGGTGGACAAGCGTACTGCTATTACATACATTGATATGGAAGGTAAATGGTATCGGGCTAGCAAAGGAGCTCCGGAACAGATTCTAGATCTATGCCCGGACAAAGACAAGATTGCTGGAAGGGTGCATTCCATTATTGACAAATTCGCCGAAAGGGGATTGCGGTCTCTTGGAGTTGCTTATCAGGAAGTTCCCGAAAAAACTAAGGATAGCCCTGGAGGTCCATGGATATTTGCTGGGTTGTTGCCCTTGTTTGATCCTCCAAGGCATGACAGTGCTGAGACCATCCGTAGAGCGCTTAACCTCGGAGTCTGTGTGAAGATGATTACAGGTGATCAGTTGGCAATTGCAAAGGAAACAGGGCGGCGACTTGGTATGGGAACAAACATGTATCCCTCCTCTTCGTTATTAGGCCGCCATGTAGAAGAACATGAAGCTCTTCCGGTGGAAGACTTGATTGAGAAGGCTGATGGCTTTGCTGGTGTCTTCCCTGAACACAAGTATGAAATTGTAAAGATTTTACAAGAAAAGAAGCACGTGGTTGGGATGACTGGAGACGGAGTTAACGATGCACCTGCTTTAAAGAAAGCAGACATTGGTATAGCAGTGGCAGATGCCACAGATGCTGCAAGAAGTGCTTCTGATATAGTCTTAACTGAGCCTGGATTGAGTGTGATAGTCAGTGCTGTCCTCACCAGTCGCGCTATATTCCAAAGAATGAAGAATTACACGATATATGCTGTCTCCATAACCATTAGGATTGTGCTTGGTTTCGTGCTTCTTGCATTGATCTGGGAATATGACTTCCCACCTTTCATGGTTCTGATAATAGCCATACTGAATGACGGAACCATCATGACAATTTCGCAGGATCGCGTAAAGCCCTCCCCAAAGCCTGATAGTTGGAAGCTGAAAGAGATATTTGCTACTGGCATTGTCATTGGCACATACCTTGCTTTAGTTACGGTATTATTCTACTGGGTTGTTGTTGAAACTGACTTCTTTGAGAGCACCTTCAATGTAAGAGATTTATCGAGCAGTAGTGAGGAAGTTTCATCTGCTGTATATCTGCAAGTCAGTATCATCAGCCAGGCTCTCATATTTGTGACAAGAAGCCAGGGGTGGTCATTTCTTGAGAGGCCCGGAACAATGTTGATGTGTGCCTTTGTGTTGGCTCAACTGGTGGCAACTCTGATTGCTGTATATGCAAGAATCAGCTTTGCATATATCAGTGGCATTGGATGGGGATGGGCCGGTATCATCTGGTTGTACAGTGTGATTTTCTACATTCCTTTGGACATCATTAAATTCATAATTCGCTATGCATTGACCGGAGATGCCTGGAATCTGTTATTCGAAAGAAAGACAGCTTTTACTAGTAAGAAAGACTATGGGAAGGAAGATCGTGCGGCTAAATGGATACTTTCTCAGAAAAGCTTACAAGGGTTGCAAGACTTTGAGATCAACAGGAGCGGCAAGCGTTCTTCTTTGATTGCAGAACAGGCCAGGCGGCGCGCTGAAATTGCCAGGCTCGGGGAGATACACACTCTGAAAGGACATGTAGAATCTGTACTGAGGCTCAAGAATTTGGACCTGAGTGTCATGCAATCAGCCCATACAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000325 GO:0003006 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005911 GO:0006725 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0006996 GO:0007033 GO:0007035 GO:0007275 GO:0008150 GO:0008152 GO:0008324 GO:0008553 GO:0009058 GO:0009506 GO:0009507 GO:0009536 GO:0009698 GO:0009699 GO:0009705 GO:0009791 GO:0009987 GO:0010023 GO:0010154 GO:0010214 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0015672 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0018958 GO:0019438 GO:0019725 GO:0019748 GO:0019829 GO:0022414 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030054 GO:0030641 GO:0031090 GO:0032501 GO:0032502 GO:0034220 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044237 GO:0044249 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045851 GO:0046189 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055044 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098805 GO:0099131 GO:0099132 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

949

Amino Acids

104.45

Weight (kDa)

5.5

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 22 - 85 1.9e-13 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 134 - 233 2.9e-30 P-type ATPase actuator domain
Hydrolase PF00702 329 - 605 1.4e-12 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 377 - 497 2.3e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17260
fragaria_vesca FvH4_6g36241
malus_domestica MD09G1171300.v1.1 MD17G1155800.v1.1
prunus_persica Prupe.3G029800_v2.0.a1 Prupe.3G029800_v2.0.a1
pyrus_communis pycom09g08870 pycom17g14850
rosa_chinensis RchiOBHm_Chr2g0148211
rosa_laevigata RLG00000020327
rosa_multiflora Rmu_sc0002583.1_g000001
rosa_roxburghii Rroxscaffold_2G00098990
rosa_rugosa Rorug02G0404500
rosa_samantha Rh2BG476300 Rh2CG449900
rosa_wichuraiana Rw2G037760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1804
AasI GACNNNNNNGTC 1 cut(s) 2819
Acc36I ACCTGC 2 cut(s) 721, 1804
AccB1I GGYRCC 1 cut(s) 941
AccBSI CCGCTC 2 cut(s) 822, 2694
AccII CGCG 4 cut(s) 1088, 1923, 2097, 2736
AccIII TCCGGA 1 cut(s) 1294
AclWI GGATC 8 cut(s) 12, 20, 33, 402, 527, 1163, 1472, 2100
AcsI RAATTY 7 cut(s) 34, 188, 751, 870, 1364, 2528, 2806
AcuI CTGAAG 1 cut(s) 729
AcvI CACGTG 1 cut(s) 1759
AfaI GTAC 6 cut(s) 465, 735, 813, 1237, 2492, 2793
AfeI AGCGCT 1 cut(s) 1518
AfiI CCNNNNNNNGG 4 cut(s) 228, 747, 1057, 2754
AflIII ACRYGT 3 cut(s) 620, 1608, 2779
AhlI ACTAGT 1 cut(s) 2597
AjnI CCWGG 9 cut(s) 466, 1128, 1435, 1882, 2310, 2337, 2561, 2726, 2747
Alw21I GWGCWC 2 cut(s) 1294, 2234
Alw26I GTCTC 5 cut(s) 576, 1392, 1496, 1770, 1969
AlwI GGATC 8 cut(s) 12, 20, 33, 402, 527, 1163, 1472, 2100
AlwNI CAGNNNCTG 1 cut(s) 1847
Ama87I CYCGRG 2 cut(s) 491, 2753
Aor13HI TCCGGA 1 cut(s) 1294
Aor51HI AGCGCT 1 cut(s) 1518
AoxI GGCC 4 cut(s) 1633, 2360, 2472, 2724
ApeKI GCWGC 8 cut(s) 242, 245, 377, 686, 1103, 1112, 1145, 1847
ApoI RAATTY 7 cut(s) 34, 188, 751, 870, 1364, 2528, 2806
Asp700I GAANNNNTTC 3 cut(s) 1302, 1659, 1858
AspLEI GCGC 6 cut(s) 259, 435, 1519, 1925, 2736, 2738
AspS9I GGNCC 5 cut(s) 287, 1442, 2361, 2472, 2812
AsuC2I CCSGG 3 cut(s) 44, 1319, 2364
AsuHPI GGTGA 7 cut(s) 96, 293, 482, 574, 608, 1565, 1906
AsuII TTCGAA 2 cut(s) 87, 2577
AsuNHI GCTAGC 1 cut(s) 1280
AvaI CYCGRG 2 cut(s) 491, 2753
AvaII GGWCC 3 cut(s) 287, 1442, 2812
BamHI GGATCC 1 cut(s) 25
BanI GGYRCC 1 cut(s) 941
BanII GRGCYC 1 cut(s) 1294
BbrPI CACGTG 1 cut(s) 1759
BbsI GAAGAC 3 cut(s) 596, 1677, 1699
Bbv12I GWGCWC 2 cut(s) 1294, 2234
BbvI GCAGC 8 cut(s) 229, 257, 364, 698, 1115, 1124, 1157, 1834
BccI CCATC 9 cut(s) 422, 827, 970, 1157, 1514, 1685, 2081, 2457, 2463
BcgI CGANNNNNNTGC 6 cut(s) 472, 506, 865, 899, 1778, 1812
BciT130I CCWGG 9 cut(s) 468, 1130, 1437, 1884, 2312, 2339, 2563, 2728, 2749
BciVI GTATCC 2 cut(s) 1623, 2634
BclI TGATCA 1 cut(s) 1555
BcnI CCSGG 3 cut(s) 44, 1319, 2364
BcoDI GTCTC 5 cut(s) 576, 1392, 1496, 1770, 1969
BcuI ACTAGT 1 cut(s) 2597
BfaI CTAG 7 cut(s) 575, 1052, 1076, 1116, 1281, 1307, 2598
BfmI CTRYAG 2 cut(s) 243, 997
BfoI RGCGCY 3 cut(s) 260, 436, 1520
BfuAI ACCTGC 2 cut(s) 721, 1804
BfuI GTATCC 2 cut(s) 1623, 2634
BglII AGATCT 2 cut(s) 1072, 1309
BlpI GCTNAGC 1 cut(s) 400
Bme18I GGWCC 3 cut(s) 287, 1442, 2812
BmeT110I CYCGRG 2 cut(s) 491, 2753
BmgT120I GGNCC 5 cut(s) 287, 1442, 2361, 2472, 2812
BmiI GGNNCC 5 cut(s) 27, 218, 288, 943, 2071
BmrI ACTGGG 2 cut(s) 803, 2209
BmtI GCTAGC 1 cut(s) 1284
BmuI ACTGGG 2 cut(s) 803, 2209
BpiI GAAGAC 3 cut(s) 596, 1677, 1699
BpmI CTGGAG 3 cut(s) 1142, 1458, 1794
Bpu1102I GCTNAGC 1 cut(s) 400
Bpu14I TTCGAA 2 cut(s) 87, 2577
BpuEI CTTGAG 2 cut(s) 2375, 2786
BpuMI CCSGG 3 cut(s) 44, 1319, 2364
Bsa29I ATCGAT 1 cut(s) 54
BsaAI YACGTR 1 cut(s) 1759
BsaBI GATNNNNATC 1 cut(s) 317
BsaI GGTCTC 2 cut(s) 1392, 1496
BsaJI CCNNGG 5 cut(s) 1435, 1445, 1484, 1525, 2338
BsaWI WCCGGW 6 cut(s) 659, 1010, 1222, 1294, 1663, 2552
BsaXI ACNNNNNCTCC 4 cut(s) 1521, 1551, 1767, 1797
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 747, 1057, 2754
Bse118I RCCGGY 1 cut(s) 2474
Bse1I ACTGG 8 cut(s) 550, 809, 1125, 1777, 1917, 2155, 2204, 2406
Bse8I GATNNNNATC 1 cut(s) 317
BseAI TCCGGA 1 cut(s) 1294
BseBI CCWGG 9 cut(s) 468, 1130, 1437, 1884, 2312, 2339, 2563, 2728, 2749
BseCI ATCGAT 1 cut(s) 54
BseDI CCNNGG 5 cut(s) 1435, 1445, 1484, 1525, 2338
BseGI GGATG 7 cut(s) 838, 963, 994, 1506, 1773, 2468, 2474
BseJI GATNNNNATC 1 cut(s) 317
BseLI CCNNNNNNNGG 4 cut(s) 228, 747, 1057, 2754
BseMII CTCAG 6 cut(s) 86, 1491, 1869, 2663, 2786, 2807
BseNI ACTGG 8 cut(s) 550, 809, 1125, 1777, 1917, 2155, 2204, 2406
BsePI GCGCGC 1 cut(s) 2734
BseRI GAGGAG 2 cut(s) 371, 1609
BseXI GCAGC 8 cut(s) 229, 257, 364, 698, 1115, 1124, 1157, 1834
BseYI CCCAGC 1 cut(s) 1457
Bsh1236I CGCG 4 cut(s) 1088, 1923, 2097, 2736
Bsh1285I CGRYCG 1 cut(s) 879
BshFI GGCC 4 cut(s) 1635, 2362, 2474, 2726
BshNI GGYRCC 1 cut(s) 941
BshVI ATCGAT 1 cut(s) 54
BsiEI CGRYCG 1 cut(s) 879
BsiHKAI GWGCWC 2 cut(s) 1294, 2234
BsiHKCI CYCGRG 2 cut(s) 491, 2753
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 4 cut(s) 228, 747, 1057, 2754
BsmAI GTCTC 5 cut(s) 576, 1392, 1496, 1770, 1969
BsmBI CGTCTC 1 cut(s) 1770
BsmFI GGGAC 1 cut(s) 509
BsmI GAATGC 2 cut(s) 379, 1348
BsnI GGCC 4 cut(s) 1635, 2362, 2474, 2726
Bso31I GGTCTC 2 cut(s) 1392, 1496
BsoBI CYCGRG 2 cut(s) 491, 2753
Bsp119I TTCGAA 2 cut(s) 87, 2577
Bsp1286I GDGCHC 2 cut(s) 1294, 2234
Bsp13I TCCGGA 1 cut(s) 1294
Bsp1407I TGTACA 1 cut(s) 2490
Bsp1720I GCTNAGC 1 cut(s) 400
Bsp19I CCATGG 1 cut(s) 1445
BspANI GGCC 4 cut(s) 1635, 2362, 2474, 2726
BspCNI CTCAG 6 cut(s) 87, 1492, 1870, 2662, 2787, 2808
BspDI ATCGAT 1 cut(s) 54
BspEI TCCGGA 1 cut(s) 1294
BspFNI CGCG 4 cut(s) 1088, 1923, 2097, 2736
BspHI TCATGA 1 cut(s) 2076
BspLI GGNNCC 5 cut(s) 27, 218, 288, 943, 2071
BspMAI CTGCAG 2 cut(s) 247, 1001
BspMI ACCTGC 2 cut(s) 721, 1804
BspOI GCTAGC 1 cut(s) 1284
BspPI GGATC 8 cut(s) 12, 20, 33, 402, 527, 1163, 1472, 2100
BspQI GCTCTTC 1 cut(s) 1665
BspT104I TTCGAA 2 cut(s) 87, 2577
BspT107I GGYRCC 1 cut(s) 941
BspTNI GGTCTC 2 cut(s) 1392, 1496
BsrBI CCGCTC 2 cut(s) 822, 2694
BsrFI RCCGGY 1 cut(s) 2474
BsrGI TGTACA 1 cut(s) 2490
BsrI ACTGG 8 cut(s) 550, 809, 1125, 1777, 1917, 2155, 2204, 2406
BssAI RCCGGY 1 cut(s) 2474
BssECI CCNNGG 5 cut(s) 1435, 1445, 1484, 1525, 2338
BssHII GCGCGC 1 cut(s) 2734
BssT1I CCWWGG 2 cut(s) 1445, 1484
Bst2UI CCWGG 9 cut(s) 468, 1130, 1437, 1884, 2312, 2339, 2563, 2728, 2749
Bst4CI ACNGT 6 cut(s) 892, 1039, 1496, 2188, 2495, 2842
Bst6I CTCTTC 4 cut(s) 165, 966, 1627, 1665
BstAPI GCANNNNNTGC 3 cut(s) 374, 1210, 1856
BstAUI TGTACA 1 cut(s) 2490
BstBAI YACGTR 1 cut(s) 1759
BstBI TTCGAA 2 cut(s) 87, 2577
BstC8I GCNNGC 4 cut(s) 1164, 1282, 2699, 2736
BstDSI CCRYGG 1 cut(s) 1445
BstENI CCTNNNNNAGG 1 cut(s) 1055
BstF5I GGATG 7 cut(s) 838, 963, 994, 1506, 1773, 2468, 2474
BstFNI CGCG 4 cut(s) 1088, 1923, 2097, 2736
BstH2I RGCGCY 3 cut(s) 260, 436, 1520
BstHHI GCGC 6 cut(s) 259, 435, 1519, 1925, 2736, 2738
BstMAI GTCTC 5 cut(s) 576, 1392, 1496, 1770, 1969
BstMCI CGRYCG 1 cut(s) 879
BstNI CCWGG 9 cut(s) 468, 1130, 1437, 1884, 2312, 2339, 2563, 2728, 2749
BstNSI RCATGY 2 cut(s) 1612, 2783
BstSFI CTRYAG 2 cut(s) 243, 997
BstUI CGCG 4 cut(s) 1088, 1923, 2097, 2736
BstV1I GCAGC 8 cut(s) 229, 257, 364, 698, 1115, 1124, 1157, 1834
BstV2I GAAGAC 3 cut(s) 596, 1677, 1699
BstX2I RGATCY 5 cut(s) 4, 25, 532, 1072, 1309
BstYI RGATCY 5 cut(s) 4, 25, 532, 1072, 1309
Bsu15I ATCGAT 1 cut(s) 54
BsuI GTATCC 2 cut(s) 1623, 2634
BsuRI GGCC 4 cut(s) 1635, 2362, 2474, 2726
BsuTUI ATCGAT 1 cut(s) 54
BtgI CCRYGG 1 cut(s) 1445
BtsCI GGATG 7 cut(s) 838, 963, 994, 1506, 1773, 2468, 2474
BtsI GCAGTG 2 cut(s) 1006, 1833
BtsIMutI CAGTG 8 cut(s) 705, 1006, 1035, 1501, 1833, 1909, 2458, 2500
BveI ACCTGC 2 cut(s) 721, 1804
Cac8I GCNNGC 4 cut(s) 1164, 1282, 2699, 2736
CaiI CAGNNNCTG 1 cut(s) 1847
CciI TCATGA 1 cut(s) 2076
CfoI GCGC 6 cut(s) 259, 435, 1519, 1925, 2736, 2738
Cfr10I RCCGGY 1 cut(s) 2474
Cfr13I GGNCC 5 cut(s) 287, 1442, 2361, 2472, 2812
ClaI ATCGAT 1 cut(s) 54
CseI GACGC 2 cut(s) 1094, 1142
CsiI ACCWGGT 1 cut(s) 466
Csp6I GTAC 6 cut(s) 464, 734, 812, 1236, 2491, 2792
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviQI GTAC 6 cut(s) 464, 734, 812, 1236, 2491, 2792
DraI TTTAAA 1 cut(s) 1803
DrdI GACNNNNNNGTC 1 cut(s) 2819
DseDI GACNNNNNNGTC 1 cut(s) 2819
Eam1104I CTCTTC 4 cut(s) 165, 966, 1627, 1665
EarI CTCTTC 4 cut(s) 165, 966, 1627, 1665
Ecl136II GAGCTC 1 cut(s) 1292
Eco130I CCWWGG 2 cut(s) 1445, 1484
Eco24I GRGCYC 1 cut(s) 1294
Eco31I GGTCTC 2 cut(s) 1392, 1496
Eco47I GGWCC 3 cut(s) 287, 1442, 2812
Eco47III AGCGCT 1 cut(s) 1518
Eco53kI GAGCTC 1 cut(s) 1292
Eco57I CTGAAG 1 cut(s) 729
Eco72I CACGTG 1 cut(s) 1759
Eco88I CYCGRG 2 cut(s) 491, 2753
EcoICRI GAGCTC 1 cut(s) 1292
EcoNI CCTNNNNNAGG 1 cut(s) 1055
EcoO109I RGGNCCY 1 cut(s) 287
EcoRI GAATTC 1 cut(s) 870
EcoRII CCWGG 9 cut(s) 466, 1128, 1435, 1882, 2310, 2337, 2561, 2726, 2747
EcoT14I CCWWGG 2 cut(s) 1445, 1484
EcoT22I ATGCAT 2 cut(s) 454, 2548
EcoT38I GRGCYC 1 cut(s) 1294
ErhI CCWWGG 2 cut(s) 1445, 1484
Esp3I CGTCTC 1 cut(s) 1770
FalI AAGNNNNNCTT 2 cut(s) 1843, 1875
FaqI GGGAC 1 cut(s) 509
FauI CCCGC 1 cut(s) 1180
FbaI TGATCA 1 cut(s) 1555
FokI GGATG 7 cut(s) 845, 970, 1001, 1493, 1780, 2475, 2481
FriOI GRGCYC 1 cut(s) 1294
FspBI CTAG 7 cut(s) 575, 1052, 1076, 1116, 1281, 1307, 2598
GlaI GCGC 6 cut(s) 258, 434, 1518, 1924, 2735, 2737
GsaI CCCAGC 1 cut(s) 1461
GsuI CTGGAG 3 cut(s) 1142, 1458, 1794
HaeII RGCGCY 3 cut(s) 260, 436, 1520
HaeIII GGCC 4 cut(s) 1635, 2362, 2474, 2726
HgaI GACGC 2 cut(s) 1094, 1142
HhaI GCGC 6 cut(s) 259, 435, 1519, 1925, 2736, 2738
Hin6I GCGC 6 cut(s) 257, 433, 1517, 1923, 2734, 2736
HinP1I GCGC 6 cut(s) 257, 433, 1517, 1923, 2734, 2736
HincII GTYRAC 3 cut(s) 547, 667, 1786
HindII GTYRAC 3 cut(s) 547, 667, 1786
HindIII AAGCTT 2 cut(s) 164, 2656
HpaI GTTAAC 2 cut(s) 667, 1786
HphI GGTGA 7 cut(s) 96, 293, 482, 574, 608, 1565, 1906
Hpy166II GTNNAC 5 cut(s) 547, 667, 700, 1228, 1786
Hpy8I GTNNAC 5 cut(s) 547, 667, 700, 1228, 1786
HpyAV CCTTC 9 cut(s) 521, 675, 1168, 1225, 1256, 1335, 1678, 2245, 2611
HpyCH4III ACNGT 6 cut(s) 892, 1039, 1496, 2188, 2495, 2842
HpyCH4IV ACGT 2 cut(s) 620, 1758
HpySE526I ACGT 2 cut(s) 620, 1758
HspAI GCGC 6 cut(s) 257, 433, 1517, 1923, 2734, 2736
Kpn2I TCCGGA 1 cut(s) 1294
Ksp22I TGATCA 1 cut(s) 1555
KspAI GTTAAC 2 cut(s) 667, 1786
LguI GCTCTTC 1 cut(s) 1665
LmnI GCTCC 4 cut(s) 409, 1289, 1297, 2691
Lsp1109I GCAGC 8 cut(s) 229, 257, 364, 698, 1115, 1124, 1157, 1834
MabI ACCWGGT 1 cut(s) 466
MaeI CTAG 7 cut(s) 575, 1052, 1076, 1116, 1281, 1307, 2598
MaeII ACGT 2 cut(s) 620, 1758
MaeIII GTNAC 6 cut(s) 470, 580, 901, 1001, 2182, 2326
MbiI CCGCTC 2 cut(s) 822, 2694
MfeI CAATTG 2 cut(s) 966, 1566
MflI RGATCY 5 cut(s) 4, 25, 532, 1072, 1309
MhlI GDGCHC 2 cut(s) 1294, 2234
MlyI GAGTC 3 cut(s) 578, 695, 1539
MmeI TCCRAC 2 cut(s) 697, 2105
Mph1103I ATGCAT 2 cut(s) 454, 2548
MroI TCCGGA 1 cut(s) 1294
MroXI GAANNNNTTC 3 cut(s) 1302, 1659, 1858
MseI TTAA 9 cut(s) 159, 486, 666, 860, 1521, 1785, 1802, 1874, 2526
MslI CAYNNNNRTG 5 cut(s) 800, 1256, 1494, 1722, 2324
MspA1I CMGCKG 1 cut(s) 248
MunI CAATTG 2 cut(s) 966, 1566
Mva1269I GAATGC 2 cut(s) 379, 1348
MvaI CCWGG 9 cut(s) 468, 1130, 1437, 1884, 2312, 2339, 2563, 2728, 2749
MvnI CGCG 4 cut(s) 1088, 1923, 2097, 2736
NciI CCSGG 3 cut(s) 44, 1319, 2364
NcoI CCATGG 1 cut(s) 1445
NheI GCTAGC 1 cut(s) 1280
NlaIV GGNNCC 5 cut(s) 27, 218, 288, 943, 2071
NmuCI GTSAC 5 cut(s) 470, 580, 901, 1001, 2326
NsiI ATGCAT 2 cut(s) 454, 2548
NspI RCATGY 2 cut(s) 1612, 2783
NspV TTCGAA 2 cut(s) 87, 2577
PagI TCATGA 1 cut(s) 2076
PaqCI CACCTGC 1 cut(s) 1804
PauI GCGCGC 1 cut(s) 2734
PciI ACATGT 2 cut(s) 1608, 2779
PciSI GCTCTTC 1 cut(s) 1665
PcsI WCGNNNNNNNCGW 1 cut(s) 1785
PctI GAATGC 2 cut(s) 379, 1348
PdmI GAANNNNTTC 3 cut(s) 1302, 1659, 1858
PfeI GAWTC 7 cut(s) 784, 837, 1027, 1303, 2433, 2566, 2786
Ple19I CGATCG 1 cut(s) 879
PleI GAGTC 3 cut(s) 577, 695, 1538
PmaCI CACGTG 1 cut(s) 1759
PmlI CACGTG 1 cut(s) 1759
PpsI GAGTC 3 cut(s) 577, 695, 1538
Ppu21I YACGTR 1 cut(s) 1759
PpuMI RGGWCCY 1 cut(s) 287
PscI ACATGT 2 cut(s) 1608, 2779
Psp124BI GAGCTC 1 cut(s) 1294
Psp5II RGGWCCY 1 cut(s) 287
Psp6I CCWGG 9 cut(s) 466, 1128, 1435, 1882, 2310, 2337, 2561, 2726, 2747
PspCI CACGTG 1 cut(s) 1759
PspFI CCCAGC 1 cut(s) 1457
PspGI CCWGG 9 cut(s) 466, 1128, 1435, 1882, 2310, 2337, 2561, 2726, 2747
PspN4I GGNNCC 5 cut(s) 27, 218, 288, 943, 2071
PspPI GGNCC 5 cut(s) 287, 1442, 2361, 2472, 2812
PspPPI RGGWCCY 1 cut(s) 287
PstI CTGCAG 2 cut(s) 247, 1001
PstNI CAGNNNCTG 1 cut(s) 1847
PsuI RGATCY 5 cut(s) 4, 25, 532, 1072, 1309
PteI GCGCGC 1 cut(s) 2734
PvuI CGATCG 1 cut(s) 879
PvuII CAGCTG 1 cut(s) 248
RsaI GTAC 6 cut(s) 465, 735, 813, 1237, 2492, 2793
RsaNI GTAC 6 cut(s) 464, 734, 812, 1236, 2491, 2792
RseI CAYNNNNRTG 5 cut(s) 800, 1256, 1494, 1722, 2324
SacI GAGCTC 1 cut(s) 1294
SapI GCTCTTC 1 cut(s) 1665
SaqAI TTAA 9 cut(s) 159, 486, 666, 860, 1521, 1785, 1802, 1874, 2526
Sau96I GGNCC 5 cut(s) 287, 1442, 2361, 2472, 2812
SchI GAGTC 3 cut(s) 578, 695, 1539
SduI GDGCHC 2 cut(s) 1294, 2234
SexAI ACCWGGT 1 cut(s) 466
SfcI CTRYAG 2 cut(s) 243, 997
SfuI TTCGAA 2 cut(s) 87, 2577
SinI GGWCC 3 cut(s) 287, 1442, 2812
SmiMI CAYNNNNRTG 5 cut(s) 800, 1256, 1494, 1722, 2324
SmlI CTYRAG 2 cut(s) 2354, 2801
SmoI CTYRAG 2 cut(s) 2354, 2801
SpeI ACTAGT 1 cut(s) 2597
SspMI CTAG 7 cut(s) 575, 1052, 1076, 1116, 1281, 1307, 2598
SstI GAGCTC 1 cut(s) 1294
StyI CCWWGG 2 cut(s) 1445, 1484
TaaI ACNGT 6 cut(s) 892, 1039, 1496, 2188, 2495, 2842
TaiI ACGT 2 cut(s) 623, 1761
TaqI TCGA 6 cut(s) 54, 87, 925, 1056, 2255, 2577
TatI WGTACW 3 cut(s) 811, 2490, 2791
TauI GCSGC 5 cut(s) 1588, 1638, 2633, 2697, 2734
TfiI GAWTC 7 cut(s) 784, 837, 1027, 1303, 2433, 2566, 2786
Tru1I TTAA 9 cut(s) 159, 486, 666, 860, 1521, 1785, 1802, 1874, 2526
Tru9I TTAA 9 cut(s) 159, 486, 666, 860, 1521, 1785, 1802, 1874, 2526
TscAI CASTG 8 cut(s) 712, 1006, 1042, 1501, 1833, 1909, 2458, 2500
TseFI GTSAC 5 cut(s) 470, 580, 901, 1001, 2326
TseI GCWGC 8 cut(s) 242, 245, 377, 686, 1103, 1112, 1145, 1847
Tsp45I GTSAC 5 cut(s) 470, 580, 901, 1001, 2326
TspDTI ATGAA 8 cut(s) 340, 615, 1668, 1740, 1955, 2026, 2265, 2521
TspGWI ACGGA 4 cut(s) 194, 1499, 1794, 2082
TspRI CASTG 8 cut(s) 712, 1006, 1042, 1501, 1833, 1909, 2458, 2500
VpaK11BI GGWCC 3 cut(s) 287, 1442, 2812
XagI CCTNNNNNAGG 1 cut(s) 1055
XapI RAATTY 7 cut(s) 34, 188, 751, 870, 1364, 2528, 2806
XbaI TCTAGA 2 cut(s) 1075, 1306
XceI RCATGY 2 cut(s) 1612, 2783
XmnI GAANNNNTTC 3 cut(s) 1302, 1659, 1858
XspI CTAG 7 cut(s) 575, 1052, 1076, 1116, 1281, 1307, 2598
Zsp2I ATGCAT 2 cut(s) 454, 2548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.