MD09G1228700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014097
Physical Location & Seq
Forward (+)
28253864 .. 28257402
3539 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1228700.v1.1.491

Sequence Viewer

Length: 927 bp
ATGGCCAGCAGCGCCGTCGCACTCTCTAGCTCCACTCTTTGCCACCTCACTAGAAAACCCACTTCACTGACTTCAACACTCAGCTTAAGAATCAGAGCCAAGGCCTCCACAGCCATGGCTGCGGCCTCCGCCGCCAAAACCAAGGTGGTACCTGCAGTGATAGTGGGCGGTGGAAGGGTCGGAAGGGCCTTGGAGGGGATGGGGAATGGCTGTGACTTGCTGGTGAAAAGAGGAGAGCCCGTGCCTCTTGATTTCCAAGGTCCAATTTTGGTTTGCACTAGGAATGATGATCTTGAAGCTGTTCTTGAAGCCACTCCAAGGCCTAGATGGAACGATTTGGTTTTTTTCCAGAATGGAATGCTGGAACCATGGTTTGAAAGTAAAGTTCTTGGTGATGCTGACCAAGTCCTAGCATATTTTGCTGTCTCCAAGCTTGGAGAACCTCCTGTTGATGGAAAGACTGATACCAATCCTGAAGGACTGACTGCGTCATATGGGAAGTGGGCATTGGCAGTAGCTGATAGATTACATGCTGGAGGCCTCACTTGCAAGGTTCTCAACAAGGAAGCATTTCAGAAGCAGATGTTAGAGAAGTTGATCTGGATTTCAGCATTCATGCTTGTCGGAGCTCGTCATCCAGGAACAACTGTAGGTGGTGTAGAGAAAGTATACCGCTCTGAGGTGTCTAGTCTCATTGCAGAGCTTGCCTCTGCAGCTGCTGCAGAGAAGGGGTTGGTATTTGAAGAAGCCATGGAGGACAGATTATGTGCATACTCACGCGCGGTTGCACACTTTCCTACTGCAGTTAAAGAGTTCAAATGGAGAAATGGTTGGTTTTATTCTCTAACCGAAAAGGCAATTGCCGAAGGAAGGCCGGATCCCTGTCCGCTCCATACGGCATGGCTCAAAGATTTGAAAGTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

309

Amino Acids

33.2

Weight (kDa)

8.46

Isoelectric Point (pI)

25.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16080
fragaria_vesca FvH4_6g28180
malus_domestica MD09G1228700.v1.1 MD17G1227100.v1.1
prunus_persica Prupe.3G104600_v2.0.a1
pyrus_communis pycom09g14760
rosa_chinensis RchiOBHm_Chr2g0132161
rosa_laevigata RLG00000019233 RLG00000019238
rosa_multiflora Rmu_sc0002005.1_g000013 Rmu_sc0009538.1_g000008
rosa_roxburghii Rroxscaffold_2G00112350
rosa_rugosa Rorug02G0302700
rosa_samantha Rh2AG355800 Rh2BG361800 Rh2DG378900
rosa_wichuraiana Rw2G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 160
Acc65I GGTACC 1 cut(s) 148
AccB1I GGYRCC 1 cut(s) 148
AccBSI CCGCTC 2 cut(s) 675, 889
AccI GTMKAC 1 cut(s) 669
AccII CGCG 2 cut(s) 780, 782
AciI CCGC 7 cut(s) 122, 129, 132, 168, 673, 782, 887
AclWI GGATC 2 cut(s) 872, 885
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 495
AfaI GTAC 1 cut(s) 150
AfiI CCNNNNNNNGG 5 cut(s) 195, 318, 452, 679, 870
AflII CTTAAG 1 cut(s) 85
AgsI TTSAA 7 cut(s) 75, 296, 308, 377, 743, 817, 916
AjnI CCWGG 1 cut(s) 637
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AluBI AGCT 8 cut(s) 30, 84, 299, 433, 518, 629, 703, 716
AluI AGCT 8 cut(s) 30, 84, 299, 433, 518, 629, 703, 716
Alw21I GWGCWC 1 cut(s) 631
Alw26I GTCTC 2 cut(s) 430, 695
AlwI GGATC 2 cut(s) 872, 885
AlwNI CAGNNNCTG 2 cut(s) 518, 719
AoxI GGCC 7 cut(s) 3, 102, 123, 186, 320, 538, 872
ApeKI GCWGC 5 cut(s) 9, 119, 713, 716, 719
Asp700I GAANNNNTTC 2 cut(s) 300, 570
Asp718I GGTACC 1 cut(s) 148
AspLEI GCGC 2 cut(s) 14, 782
AspS9I GGNCC 2 cut(s) 186, 260
AsuHPI GGTGA 2 cut(s) 235, 404
AvaII GGWCC 1 cut(s) 260
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 877
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 2 cut(s) 240, 631
Bbv12I GWGCWC 1 cut(s) 631
BbvI GCAGC 5 cut(s) 21, 106, 703, 706, 725
BccI CCATC 3 cut(s) 193, 321, 446
BceAI ACGGC 1 cut(s) 912
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 639
BcoDI GTCTC 2 cut(s) 430, 695
BfaI CTAG 7 cut(s) 27, 51, 279, 324, 410, 687, 925
BfmI CTRYAG 5 cut(s) 153, 648, 711, 720, 801
BfoI RGCGCY 1 cut(s) 15
BfrI CTTAAG 1 cut(s) 85
BfuAI ACCTGC 1 cut(s) 160
BisI GCNGC 7 cut(s) 10, 120, 123, 132, 714, 717, 720
BlsI GCNGC 7 cut(s) 11, 121, 124, 133, 715, 718, 721
Bme1390I CCNGG 1 cut(s) 639
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 186, 260
BmiI GGNNCC 3 cut(s) 150, 366, 879
BmrFI CCNGG 1 cut(s) 639
BmsI GCATC 1 cut(s) 385
BplI GAGNNNNNCTC 2 cut(s) 692, 724
BpmI CTGGAG 1 cut(s) 555
BsaBI GATNNNNATC 1 cut(s) 468
BsaJI CCNNGG 8 cut(s) 99, 114, 141, 189, 256, 317, 368, 750
BsaXI ACNNNNNCTCC 4 cut(s) 225, 255, 814, 844
Bsc4I CCNNNNNNNGG 5 cut(s) 195, 318, 452, 679, 870
Bse3DI GCAATG 1 cut(s) 693
Bse8I GATNNNNATC 1 cut(s) 468
BseBI CCWGG 1 cut(s) 639
BseDI CCNNGG 8 cut(s) 99, 114, 141, 189, 256, 317, 368, 750
BseGI GGATG 2 cut(s) 204, 634
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 5 cut(s) 195, 318, 452, 679, 870
BseMI GCAATG 1 cut(s) 693
BseMII CTCAG 2 cut(s) 94, 669
BseRI GAGGAG 1 cut(s) 246
BseXI GCAGC 5 cut(s) 21, 106, 703, 706, 725
Bsh1236I CGCG 2 cut(s) 780, 782
BshFI GGCC 7 cut(s) 5, 104, 125, 188, 322, 540, 874
BshNI GGYRCC 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 631
BsiSI CCGG 1 cut(s) 875
BslI CCNNNNNNNGG 5 cut(s) 195, 318, 452, 679, 870
BsmAI GTCTC 2 cut(s) 430, 695
BsmI GAATGC 2 cut(s) 363, 611
BsnI GGCC 7 cut(s) 5, 104, 125, 188, 322, 540, 874
Bsp1286I GDGCHC 2 cut(s) 240, 631
Bsp143I GATC 3 cut(s) 289, 597, 877
Bsp19I CCATGG 3 cut(s) 114, 368, 750
BspACI CCGC 7 cut(s) 122, 129, 132, 168, 673, 782, 887
BspANI GGCC 7 cut(s) 5, 104, 125, 188, 322, 540, 874
BspCNI CTCAG 2 cut(s) 93, 670
BspFNI CGCG 2 cut(s) 780, 782
BspLI GGNNCC 3 cut(s) 150, 366, 879
BspMAI CTGCAG 4 cut(s) 157, 715, 724, 805
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 2 cut(s) 872, 885
BspT107I GGYRCC 1 cut(s) 148
BspTI CTTAAG 1 cut(s) 85
BsrBI CCGCTC 2 cut(s) 675, 889
BsrDI GCAATG 1 cut(s) 693
BssECI CCNNGG 8 cut(s) 99, 114, 141, 189, 256, 317, 368, 750
BssMI GATC 3 cut(s) 289, 597, 877
BssNAI GTATAC 1 cut(s) 670
BssT1I CCWWGG 8 cut(s) 99, 114, 141, 189, 256, 317, 368, 750
Bst1107I GTATAC 1 cut(s) 670
Bst2UI CCWGG 1 cut(s) 639
Bst4CI ACNGT 1 cut(s) 649
BstAFI CTTAAG 1 cut(s) 85
BstAPI GCANNNNNTGC 3 cut(s) 419, 704, 719
BstC8I GCNNGC 2 cut(s) 7, 705
BstDEI CTNAG 2 cut(s) 80, 678
BstDSI CCRYGG 3 cut(s) 114, 368, 750
BstF5I GGATG 2 cut(s) 204, 634
BstFNI CGCG 2 cut(s) 780, 782
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 2 cut(s) 14, 782
BstKTI GATC 3 cut(s) 292, 600, 880
BstMAI GTCTC 2 cut(s) 430, 695
BstMBI GATC 3 cut(s) 289, 597, 877
BstNI CCWGG 1 cut(s) 639
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 1 cut(s) 637
BstSFI CTRYAG 5 cut(s) 153, 648, 711, 720, 801
BstUI CGCG 2 cut(s) 780, 782
BstV1I GCAGC 5 cut(s) 21, 106, 703, 706, 725
BstX2I RGATCY 1 cut(s) 877
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 1 cut(s) 877
BstZ17I GTATAC 1 cut(s) 670
BsuRI GGCC 7 cut(s) 5, 104, 125, 188, 322, 540, 874
BtgI CCRYGG 3 cut(s) 114, 368, 750
BtsCI GGATG 2 cut(s) 204, 634
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 2 cut(s) 65, 162
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 2 cut(s) 7, 705
CaiI CAGNNNCTG 2 cut(s) 518, 719
CfoI GCGC 2 cut(s) 14, 782
Cfr13I GGNCC 2 cut(s) 186, 260
CseI GACGC 1 cut(s) 477
Csp6I GTAC 1 cut(s) 149
CviAII CATG 6 cut(s) 115, 369, 530, 616, 751, 900
CviQI GTAC 1 cut(s) 149
DdeI CTNAG 2 cut(s) 80, 678
DpnI GATC 3 cut(s) 291, 599, 879
DpnII GATC 3 cut(s) 289, 597, 877
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 118
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 8 cut(s) 99, 114, 141, 189, 256, 317, 368, 750
Eco147I AGGCCT 3 cut(s) 104, 322, 540
Eco24I GRGCYC 2 cut(s) 240, 631
Eco47I GGWCC 1 cut(s) 260
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 1 cut(s) 495
EcoICRI GAGCTC 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 637
EcoT14I CCWWGG 8 cut(s) 99, 114, 141, 189, 256, 317, 368, 750
EcoT38I GRGCYC 2 cut(s) 240, 631
ErhI CCWWGG 8 cut(s) 99, 114, 141, 189, 256, 317, 368, 750
FaeI CATG 6 cut(s) 118, 372, 533, 619, 754, 903
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FatI CATG 6 cut(s) 114, 368, 529, 615, 750, 899
FauNDI CATATG 1 cut(s) 493
FblI GTMKAC 1 cut(s) 669
Fnu4HI GCNGC 7 cut(s) 10, 120, 123, 132, 714, 717, 720
FokI GGATG 2 cut(s) 211, 621
FriOI GRGCYC 2 cut(s) 240, 631
Fsp4HI GCNGC 7 cut(s) 10, 120, 123, 132, 714, 717, 720
FspBI CTAG 7 cut(s) 27, 51, 279, 324, 410, 687, 925
GlaI GCGC 2 cut(s) 13, 781
GluI GCNGC 7 cut(s) 10, 120, 123, 132, 714, 717, 720
GsuI CTGGAG 1 cut(s) 555
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 7 cut(s) 5, 104, 125, 188, 322, 540, 874
HapII CCGG 1 cut(s) 875
HgaI GACGC 1 cut(s) 477
HhaI GCGC 2 cut(s) 14, 782
Hin1II CATG 6 cut(s) 118, 372, 533, 619, 754, 903
Hin6I GCGC 2 cut(s) 12, 780
HinP1I GCGC 2 cut(s) 12, 780
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 1 cut(s) 90
HpaII CCGG 1 cut(s) 875
HphI GGTGA 2 cut(s) 235, 404
Hpy166II GTNNAC 1 cut(s) 670
Hpy188I TCNGA 5 cut(s) 95, 182, 576, 626, 679
Hpy188III TCNNGA 6 cut(s) 248, 293, 305, 349, 473, 601
Hpy8I GTNNAC 1 cut(s) 670
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 6 cut(s) 168, 177, 470, 721, 860, 864
HpyCH4III ACNGT 1 cut(s) 649
HpyCH4V TGCA 9 cut(s) 155, 276, 549, 698, 713, 722, 770, 788, 803
HpyF3I CTNAG 2 cut(s) 80, 678
Hsp92II CATG 6 cut(s) 118, 372, 533, 619, 754, 903
HspAI GCGC 2 cut(s) 12, 780
KpnI GGTACC 1 cut(s) 152
Kzo9I GATC 3 cut(s) 289, 597, 877
LmnI GCTCC 3 cut(s) 35, 626, 894
Lsp1109I GCAGC 5 cut(s) 21, 106, 703, 706, 725
LweI GCATC 1 cut(s) 385
MaeI CTAG 7 cut(s) 27, 51, 279, 324, 410, 687, 925
MaeIII GTNAC 1 cut(s) 212
MalI GATC 3 cut(s) 291, 599, 879
MbiI CCGCTC 2 cut(s) 675, 889
MboI GATC 3 cut(s) 289, 597, 877
MboII GAAGA 1 cut(s) 755
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 1 cut(s) 877
MhlI GDGCHC 2 cut(s) 240, 631
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 264, 858
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 160, 604
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 300, 570
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 86, 807
MslI CAYNNNNRTG 1 cut(s) 113
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 716
MspCI CTTAAG 1 cut(s) 85
MspI CCGG 1 cut(s) 875
MspR9I CCNGG 1 cut(s) 639
MunI CAATTG 1 cut(s) 858
Mva1269I GAATGC 2 cut(s) 363, 611
MvaI CCWGG 1 cut(s) 639
MvnI CGCG 2 cut(s) 780, 782
NcoI CCATGG 3 cut(s) 114, 368, 750
NdeI CATATG 1 cut(s) 493
NdeII GATC 3 cut(s) 289, 597, 877
NlaIII CATG 6 cut(s) 118, 372, 533, 619, 754, 903
NlaIV GGNNCC 3 cut(s) 150, 366, 879
NmuCI GTSAC 1 cut(s) 212
NspI RCATGY 1 cut(s) 533
PceI AGGCCT 3 cut(s) 104, 322, 540
PctI GAATGC 2 cut(s) 363, 611
PdmI GAANNNNTTC 2 cut(s) 300, 570
PfeI GAWTC 1 cut(s) 90
PflFI GACNNNGTC 2 cut(s) 404, 487
PfoI TCCNGGA 1 cut(s) 637
PkrI GCNGC 7 cut(s) 11, 121, 124, 133, 715, 718, 721
Psp124BI GAGCTC 1 cut(s) 631
Psp6I CCWGG 1 cut(s) 637
PspGI CCWGG 1 cut(s) 637
PspN4I GGNNCC 3 cut(s) 150, 366, 879
PspPI GGNCC 2 cut(s) 186, 260
PstI CTGCAG 4 cut(s) 157, 715, 724, 805
PstNI CAGNNNCTG 2 cut(s) 518, 719
PsuI RGATCY 1 cut(s) 877
PsyI GACNNNGTC 2 cut(s) 404, 487
PvuII CAGCTG 1 cut(s) 716
RsaI GTAC 1 cut(s) 150
RsaNI GTAC 1 cut(s) 149
RseI CAYNNNNRTG 1 cut(s) 113
SacI GAGCTC 1 cut(s) 631
SaqAI TTAA 2 cut(s) 86, 807
SatI GCNGC 7 cut(s) 10, 120, 123, 132, 714, 717, 720
Sau3AI GATC 3 cut(s) 289, 597, 877
Sau96I GGNCC 2 cut(s) 186, 260
ScrFI CCNGG 1 cut(s) 639
SduI GDGCHC 2 cut(s) 240, 631
SfaNI GCATC 1 cut(s) 385
SfcI CTRYAG 5 cut(s) 153, 648, 711, 720, 801
SinI GGWCC 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 85
SmoI CTYRAG 1 cut(s) 85
Sse9I AATT 2 cut(s) 264, 858
SseBI AGGCCT 3 cut(s) 104, 322, 540
SsiI CCGC 7 cut(s) 122, 129, 132, 168, 673, 782, 887
SspMI CTAG 7 cut(s) 27, 51, 279, 324, 410, 687, 925
SstI GAGCTC 1 cut(s) 631
StuI AGGCCT 3 cut(s) 104, 322, 540
StyD4I CCNGG 1 cut(s) 637
StyI CCWWGG 8 cut(s) 99, 114, 141, 189, 256, 317, 368, 750
TaaI ACNGT 1 cut(s) 649
TasI AATT 2 cut(s) 264, 858
TauI GCSGC 2 cut(s) 125, 134
TfiI GAWTC 1 cut(s) 90
Tru1I TTAA 2 cut(s) 86, 807
Tru9I TTAA 2 cut(s) 86, 807
TscAI CASTG 2 cut(s) 72, 162
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 5 cut(s) 9, 119, 713, 716, 719
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 604
TspRI CASTG 2 cut(s) 72, 162
Tth111I GACNNNGTC 2 cut(s) 404, 487
Vha464I CTTAAG 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 260
XceI RCATGY 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 2 cut(s) 142, 324
XmiI GTMKAC 1 cut(s) 669
XmnI GAANNNNTTC 2 cut(s) 300, 570
XspI CTAG 7 cut(s) 27, 51, 279, 324, 410, 687, 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.