MD17G1227100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014097
Physical Location & Seq
Forward (+)
27507046 .. 27508757
1712 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1227100.v1.1.491

Sequence Viewer

Length: 570 bp
ATGCTGGAACCATGGTTTGGAAGTAAAGGTCTTGGTGATGCTGACCAAGTCCTAGCATATTTTGCTGTCTCCAAGCTTGGAGAACCTCCTGTTGATGGAAAGACTGATACCAATCCTGAAGGACTGACTGCATCATATGGGAAGTGGGCATCGACGGTAGCTGATAGATTACATGCTGGAGGCCTCACTTGCAAGGTTCTCAACAAGGAAAATTTTCAGAAGCAGATGTTAGAGAAGTTGATCTGGATTTCAGCATTCATGCTTGTTGGAGCTCGTCATCCAGGAACAACTGTAGGTGGTGTAGAGAAAGAATACCGCTCTGAGGTGTCTAGTCTCATTGCAGAGCTTGCCTCTGCAGCAGCTGCAGAGAAGGGGTTGGTATTTGAAGAAGCCATGGAGCACAGATTATGTGCATACTCACGCACGGTTGCACACTTTCCTACCGCAGTTAAAGAGTTTAAATGGAGAAACGGTTGGTTTTATTCTCTATCTGAAAAGGCAATTGCCGAAGGAAAGCCTGATCCCTGTCCGCTACATACGACATGGCTCAAAGATTTGAAAGTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

190

Amino Acids

20.78

Weight (kDa)

6.52

Isoelectric Point (pI)

13.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16080
fragaria_vesca FvH4_6g28180
malus_domestica MD09G1228700.v1.1 MD17G1227100.v1.1
prunus_persica Prupe.3G104600_v2.0.a1
pyrus_communis pycom09g14760
rosa_chinensis RchiOBHm_Chr2g0132161
rosa_laevigata RLG00000019233 RLG00000019238
rosa_multiflora Rmu_sc0002005.1_g000013 Rmu_sc0009538.1_g000008
rosa_roxburghii Rroxscaffold_2G00112350
rosa_rugosa Rorug02G0302700
rosa_samantha Rh2AG355800 Rh2BG361800 Rh2DG378900
rosa_wichuraiana Rw2G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 17
AccBSI CCGCTC 1 cut(s) 318
AciI CCGC 3 cut(s) 316, 444, 530
AclWI GGATC 1 cut(s) 515
AcsI RAATTY 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 138
AfiI CCNNNNNNNGG 3 cut(s) 17, 95, 322
AgsI TTSAA 2 cut(s) 386, 559
AjnI CCWGG 1 cut(s) 280
AjuI GAANNNNNNNTTGG 1 cut(s) 32
AluBI AGCT 5 cut(s) 76, 161, 272, 346, 362
AluI AGCT 5 cut(s) 76, 161, 272, 346, 362
Alw21I GWGCWC 2 cut(s) 274, 402
Alw26I GTCTC 2 cut(s) 73, 338
AlwI GGATC 1 cut(s) 515
AlwNI CAGNNNCTG 1 cut(s) 362
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 3 cut(s) 356, 359, 362
ApoI RAATTY 1 cut(s) 211
Asp700I GAANNNNTTC 1 cut(s) 213
AsuHPI GGTGA 1 cut(s) 47
BanII GRGCYC 1 cut(s) 274
Bbv12I GWGCWC 2 cut(s) 274, 402
BbvI GCAGC 3 cut(s) 349, 368, 371
BccI CCATC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 282
BcoDI GTCTC 2 cut(s) 73, 338
BfaI CTAG 3 cut(s) 53, 330, 568
BfmI CTRYAG 3 cut(s) 291, 354, 363
BisI GCNGC 3 cut(s) 357, 360, 363
BlsI GCNGC 3 cut(s) 358, 361, 364
Bme1390I CCNGG 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 3 cut(s) 28, 140, 158
BplI GAGNNNNNCTC 2 cut(s) 335, 367
BpmI CTGGAG 1 cut(s) 198
BsaBI GATNNNNATC 1 cut(s) 111
BsaJI CCNNGG 2 cut(s) 11, 393
BsaXI ACNNNNNCTCC 2 cut(s) 457, 487
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 95, 322
Bse3DI GCAATG 1 cut(s) 336
Bse8I GATNNNNATC 1 cut(s) 111
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 2 cut(s) 11, 393
BseGI GGATG 1 cut(s) 277
BseJI GATNNNNATC 1 cut(s) 111
BseLI CCNNNNNNNGG 3 cut(s) 17, 95, 322
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 1 cut(s) 312
BseXI GCAGC 3 cut(s) 349, 368, 371
BshFI GGCC 1 cut(s) 183
BsiHKAI GWGCWC 2 cut(s) 274, 402
BslI CCNNNNNNNGG 3 cut(s) 17, 95, 322
BsmAI GTCTC 2 cut(s) 73, 338
BsmI GAATGC 1 cut(s) 254
BsnI GGCC 1 cut(s) 183
Bsp1286I GDGCHC 2 cut(s) 274, 402
Bsp143I GATC 2 cut(s) 240, 520
Bsp19I CCATGG 2 cut(s) 11, 393
BspACI CCGC 3 cut(s) 316, 444, 530
BspANI GGCC 1 cut(s) 183
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 1 cut(s) 9
BspMAI CTGCAG 2 cut(s) 358, 367
BspPI GGATC 1 cut(s) 515
BsrBI CCGCTC 1 cut(s) 318
BsrDI GCAATG 1 cut(s) 336
BssECI CCNNGG 2 cut(s) 11, 393
BssMI GATC 2 cut(s) 240, 520
BssT1I CCWWGG 2 cut(s) 11, 393
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 4 cut(s) 157, 292, 427, 473
BstAPI GCANNNNNTGC 3 cut(s) 62, 347, 362
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 1 cut(s) 321
BstDSI CCRYGG 2 cut(s) 11, 393
BstF5I GGATG 1 cut(s) 277
BstKTI GATC 2 cut(s) 243, 523
BstMAI GTCTC 2 cut(s) 73, 338
BstMBI GATC 2 cut(s) 240, 520
BstMWI GCNNNNNNNGC 5 cut(s) 62, 189, 347, 356, 362
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 1 cut(s) 176
BstSCI CCNGG 1 cut(s) 280
BstSFI CTRYAG 3 cut(s) 291, 354, 363
BstV1I GCAGC 3 cut(s) 349, 368, 371
BsuRI GGCC 1 cut(s) 183
BtgI CCRYGG 2 cut(s) 11, 393
BtsCI GGATG 1 cut(s) 277
Cac8I GCNNGC 1 cut(s) 348
CaiI CAGNNNCTG 1 cut(s) 362
CviAII CATG 5 cut(s) 12, 173, 259, 394, 543
CviJI RGCY 9 cut(s) 76, 161, 183, 272, 346, 362, 392, 517, 547
CviKI_1 RGCY 9 cut(s) 76, 161, 183, 272, 346, 362, 392, 517, 547
DdeI CTNAG 1 cut(s) 321
DpnI GATC 2 cut(s) 242, 522
DpnII GATC 2 cut(s) 240, 520
DraI TTTAAA 1 cut(s) 460
Ecl136II GAGCTC 1 cut(s) 272
Eco130I CCWWGG 2 cut(s) 11, 393
Eco147I AGGCCT 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 274
Eco53kI GAGCTC 1 cut(s) 272
Eco57I CTGAAG 1 cut(s) 138
EcoICRI GAGCTC 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 280
EcoT14I CCWWGG 2 cut(s) 11, 393
EcoT38I GRGCYC 1 cut(s) 274
ErhI CCWWGG 2 cut(s) 11, 393
FaeI CATG 5 cut(s) 15, 176, 262, 397, 546
FatI CATG 5 cut(s) 11, 172, 258, 393, 542
FauNDI CATATG 1 cut(s) 136
Fnu4HI GCNGC 3 cut(s) 357, 360, 363
FokI GGATG 1 cut(s) 264
FriOI GRGCYC 1 cut(s) 274
Fsp4HI GCNGC 3 cut(s) 357, 360, 363
FspBI CTAG 3 cut(s) 53, 330, 568
GluI GCNGC 3 cut(s) 357, 360, 363
GsuI CTGGAG 1 cut(s) 198
HaeIII GGCC 1 cut(s) 183
Hin1II CATG 5 cut(s) 15, 176, 262, 397, 546
HindIII AAGCTT 1 cut(s) 74
HphI GGTGA 1 cut(s) 47
Hpy188I TCNGA 3 cut(s) 219, 322, 493
Hpy188III TCNNGA 2 cut(s) 116, 244
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 3 cut(s) 113, 364, 503
HpyCH4III ACNGT 4 cut(s) 157, 292, 427, 473
HpyCH4V TGCA 7 cut(s) 131, 192, 341, 356, 365, 413, 431
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 189, 347, 356, 362
HpyF3I CTNAG 1 cut(s) 321
Hsp92II CATG 5 cut(s) 15, 176, 262, 397, 546
Kzo9I GATC 2 cut(s) 240, 520
LmnI GCTCC 2 cut(s) 269, 397
LpnPI CCDG 8 cut(s) 102, 129, 162, 229, 267, 294, 531, 538
Lsp1109I GCAGC 3 cut(s) 349, 368, 371
LweI GCATC 3 cut(s) 28, 140, 158
MaeI CTAG 3 cut(s) 53, 330, 568
MalI GATC 2 cut(s) 242, 522
MbiI CCGCTC 1 cut(s) 318
MboI GATC 2 cut(s) 240, 520
MboII GAAGA 1 cut(s) 398
MfeI CAATTG 1 cut(s) 501
MhlI GDGCHC 2 cut(s) 274, 402
MluCI AATT 2 cut(s) 211, 501
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 5 cut(s) 96, 173, 194, 316, 361
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 2 cut(s) 450, 459
MspA1I CMGCKG 1 cut(s) 362
MspR9I CCNGG 1 cut(s) 282
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 1 cut(s) 254
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 5 cut(s) 62, 189, 347, 356, 362
NcoI CCATGG 2 cut(s) 11, 393
NdeI CATATG 1 cut(s) 136
NdeII GATC 2 cut(s) 240, 520
NlaIII CATG 5 cut(s) 15, 176, 262, 397, 546
NlaIV GGNNCC 1 cut(s) 9
NspI RCATGY 1 cut(s) 176
PceI AGGCCT 1 cut(s) 183
PctI GAATGC 1 cut(s) 254
PdmI GAANNNNTTC 1 cut(s) 213
PflFI GACNNNGTC 1 cut(s) 47
PflMI CCANNNNNTGG 1 cut(s) 17
PfoI TCCNGGA 1 cut(s) 280
PkrI GCNGC 3 cut(s) 358, 361, 364
Psp124BI GAGCTC 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 1 cut(s) 9
PstI CTGCAG 2 cut(s) 358, 367
PstNI CAGNNNCTG 1 cut(s) 362
PsyI GACNNNGTC 1 cut(s) 47
PvuII CAGCTG 1 cut(s) 362
SacI GAGCTC 1 cut(s) 274
SaqAI TTAA 2 cut(s) 450, 459
SatI GCNGC 3 cut(s) 357, 360, 363
Sau3AI GATC 2 cut(s) 240, 520
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 2 cut(s) 274, 402
SfaNI GCATC 3 cut(s) 28, 140, 158
SfcI CTRYAG 3 cut(s) 291, 354, 363
Sse9I AATT 2 cut(s) 211, 501
SseBI AGGCCT 1 cut(s) 183
SsiI CCGC 3 cut(s) 316, 444, 530
SspMI CTAG 3 cut(s) 53, 330, 568
SstI GAGCTC 1 cut(s) 274
StuI AGGCCT 1 cut(s) 183
StyD4I CCNGG 1 cut(s) 280
StyI CCWWGG 2 cut(s) 11, 393
TaaI ACNGT 4 cut(s) 157, 292, 427, 473
TaqI TCGA 1 cut(s) 152
TasI AATT 2 cut(s) 211, 501
Tru1I TTAA 2 cut(s) 450, 459
Tru9I TTAA 2 cut(s) 450, 459
TseI GCWGC 3 cut(s) 356, 359, 362
TspDTI ATGAA 1 cut(s) 247
Tth111I GACNNNGTC 1 cut(s) 47
Van91I CCANNNNNTGG 1 cut(s) 17
XapI RAATTY 1 cut(s) 211
XceI RCATGY 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 213
XspI CTAG 3 cut(s) 53, 330, 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.