MD09G1233100.v1.1

Dihydroorotase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010110
Physical Location & Seq
Reverse (-)
29223287 .. 29235046
11760 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1233100.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGATAAAGAGTTGGGTTTTGCCTTGCAAGGCATTGAAGTTTCCCTCATCAAATTTCGAAGGATCCAACCAGTTTCGGTGCAAAGGAGCCAAGATGGAGCTCACTATAACTCGCCCTGATGATTGGCACCTTCACCTTCGTGACGGTGAACTTCTGCAGGCGGTAGTCCCGCACAGTGCAAGTCATTTTGGAAGAGCAATTGTGATGCCTAATCTGAAACCTCCCATCACCACCACAGCTGCAGCTGTGGCTTATCGAGAATCCATCTTGAAAGCAATGCCTGCCGGTAGTGACTTCACACCACTTATGACCCTTTATTTGACTGATACCACAGAACCTAACGAGATCAAGCTCGCAAGAAGAAGTGGGGTTGTTTATGCTGTGAAGTTGTACCCTGCTGGTGCCACAACAAACTCTCAAGATGGGGTTACAGATCTCTTTGGAAGATGCCTACCTGTCCTTGAGGAGATGGCTGAACTTAATATACCTCTGCTGGTTCATGGGGAGGTTACAGATCCTGATGTTGATGTATTTGATCGTGAAAGGGTCTTCATTGACACTGTTTTAAGACCATTAATCCAAAGGCTTCCAAAGCTAAAGGTCGTGATGGAGCATATTACTACCATGGATGCGGCTAAGTTTGTAGAGTCTTGTGAAGAAGGTTCTGTAGCAGCAACTGTTACTCCACAACATCTTCTTCTGAATAGAAACTCCCTTTTCCAAGGAGGATTGCAGCCTCATAATTACTGTTTACCTGTACTCAAAAGAGAAATTCACAGACAGGCTATTGTGTCTGCTGTTACTAGTGGAAGTAAAAGATTTTTCATTGGAACTGATAGCGCTCCTCATGAGAGACGTAGAAAAGAAAGTGCTTGTGGATGTGCTGGTATATACAGTGCTCCTGTTGCTTTATCATTATACGCCAAGGTCTTTGAAGAGGCAGGTGCTATTGACAAGCTAGAAGCCTTTGTAAGCTTTAATGGCCCAGACTTCTATGGGCTTCCTCGAAACACGTCGAAGATAAAGCTGAGCAAGAATTCATTTAAGGTACCTGATTGTTTCTCTTTTCCGTTTGGAGACATTGTTCCGATGTTTGCTGGAGAAACACTTGAATGGCAGTCATCCATCAACTGA

Protein Analysis

378

Amino Acids

41.67

Weight (kDa)

7.13

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 41 - 342 1.1e-25 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22930 AT4G22930
fragaria_vesca FvH4_6g26410 FvH4_6g26410 FvH4_6g26410 FvH4_6g26410
malus_domestica MD09G1233100.v1.1
prunus_persica Prupe.3G131200_v2.0.a1
pyrus_communis pycom09g16330
rosa_chinensis RchiOBHm_Chr2g0127911
rosa_laevigata RLG00000018930
rosa_multiflora Rmu_ssc0000093.1_g000008
rosa_roxburghii Rroxscaffold_2G00116850
rosa_rugosa Rorug02G0270300 Rorug02G0270300 Rorug02G0270400
rosa_samantha Rh2AG322800 Rh2BG331700 Rh2CG310800 Rh2DG350300
rosa_wichuraiana Rw2G026180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 932
Acc36I ACCTGC 1 cut(s) 932
Acc65I GGTACC 1 cut(s) 1048
AccB1I GGYRCC 3 cut(s) 126, 401, 1048
AciI CCGC 3 cut(s) 161, 170, 632
AclWI GGATC 3 cut(s) 57, 70, 509
AcsI RAATTY 3 cut(s) 52, 771, 1036
AfaI GTAC 3 cut(s) 392, 759, 1050
AfeI AGCGCT 1 cut(s) 841
AflIII ACRYGT 1 cut(s) 1011
AgsI TTSAA 4 cut(s) 37, 271, 935, 1112
AhlI ACTAGT 1 cut(s) 803
AjiI CACGTC 1 cut(s) 1014
AleI CACNNNNGTG 1 cut(s) 138
AloI GAACNNNNNNTCC 2 cut(s) 1068, 1100
AluBI AGCT 8 cut(s) 100, 239, 245, 352, 595, 958, 975, 1027
AluI AGCT 8 cut(s) 100, 239, 245, 352, 595, 958, 975, 1027
Alw21I GWGCWC 2 cut(s) 102, 901
Alw26I GTCTC 2 cut(s) 847, 1071
AlwI GGATC 3 cut(s) 57, 70, 509
AlwNI CAGNNNCTG 2 cut(s) 518, 677
Aor51HI AGCGCT 1 cut(s) 841
AoxI GGCC 1 cut(s) 982
ApeKI GCWGC 4 cut(s) 239, 242, 671, 733
ApoI RAATTY 3 cut(s) 52, 771, 1036
ArsI GACNNNNNNTTYG 2 cut(s) 301, 333
AseI ATTAAT 1 cut(s) 575
Asp718I GGTACC 1 cut(s) 1048
AspLEI GCGC 1 cut(s) 842
AspS9I GGNCC 1 cut(s) 983
AsuHPI GGTGA 3 cut(s) 125, 158, 220
AsuII TTCGAA 1 cut(s) 57
BamHI GGATCC 1 cut(s) 62
BanI GGYRCC 3 cut(s) 126, 401, 1048
BanII GRGCYC 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 541
Bbv12I GWGCWC 2 cut(s) 102, 901
BbvI GCAGC 4 cut(s) 226, 254, 683, 745
BccI CCATC 7 cut(s) 88, 233, 272, 416, 463, 601, 1133
BcoDI GTCTC 2 cut(s) 847, 1071
BcuI ACTAGT 1 cut(s) 803
BfaI CTAG 2 cut(s) 804, 959
BfmI CTRYAG 3 cut(s) 155, 240, 666
BfoI RGCGCY 1 cut(s) 843
BfuAI ACCTGC 1 cut(s) 932
BglII AGATCT 1 cut(s) 433
BisI GCNGC 5 cut(s) 240, 243, 633, 672, 734
BlpI GCTNAGC 1 cut(s) 1028
BlsI GCNGC 5 cut(s) 241, 244, 634, 673, 735
BmgBI CACGTC 1 cut(s) 1014
BmgT120I GGNCC 1 cut(s) 983
BmiI GGNNCC 5 cut(s) 64, 88, 128, 403, 1050
BmsI GCATC 3 cut(s) 195, 437, 619
BpiI GAAGAC 1 cut(s) 541
BpmI CTGGAG 1 cut(s) 1119
Bpu1102I GCTNAGC 1 cut(s) 1028
Bpu14I TTCGAA 1 cut(s) 57
BpuEI CTTGAG 2 cut(s) 402, 482
BsaJI CCNNGG 3 cut(s) 624, 721, 924
BsaXI ACNNNNNCTCC 4 cut(s) 667, 697, 1068, 1098
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 70
Bse3DI GCAATG 1 cut(s) 282
BseDI CCNNGG 3 cut(s) 624, 721, 924
BseGI GGATG 3 cut(s) 634, 884, 1121
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 1 cut(s) 1019
BseNI ACTGG 1 cut(s) 70
BseRI GAGGAG 2 cut(s) 479, 834
BseXI GCAGC 4 cut(s) 226, 254, 683, 745
BshFI GGCC 1 cut(s) 984
BshNI GGYRCC 3 cut(s) 126, 401, 1048
BsiHKAI GWGCWC 2 cut(s) 102, 901
BsiSI CCGG 1 cut(s) 285
BslFI GGGAC 1 cut(s) 152
BsmAI GTCTC 2 cut(s) 847, 1071
BsmBI CGTCTC 1 cut(s) 847
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 1 cut(s) 984
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 102, 901
Bsp143I GATC 5 cut(s) 62, 345, 433, 514, 535
Bsp1720I GCTNAGC 1 cut(s) 1028
Bsp19I CCATGG 1 cut(s) 624
BspACI CCGC 3 cut(s) 161, 170, 632
BspANI GGCC 1 cut(s) 984
BspCNI CTCAG 1 cut(s) 1020
BspHI TCATGA 1 cut(s) 847
BspLI GGNNCC 5 cut(s) 64, 88, 128, 403, 1050
BspMAI CTGCAG 2 cut(s) 159, 244
BspMI ACCTGC 1 cut(s) 932
BspPI GGATC 3 cut(s) 57, 70, 509
BspQI GCTCTTC 1 cut(s) 187
BspT104I TTCGAA 1 cut(s) 57
BspT107I GGYRCC 3 cut(s) 126, 401, 1048
BsrDI GCAATG 1 cut(s) 282
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 1 cut(s) 70
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 3 cut(s) 624, 721, 924
BssMI GATC 5 cut(s) 62, 345, 433, 514, 535
BssT1I CCWWGG 3 cut(s) 624, 721, 924
Bst4CI ACNGT 6 cut(s) 146, 176, 562, 679, 749, 896
Bst6I CTCTTC 2 cut(s) 187, 930
BstAPI GCANNNNNTGC 1 cut(s) 281
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 3 cut(s) 159, 282, 354
BstDEI CTNAG 2 cut(s) 636, 1028
BstDSI CCRYGG 1 cut(s) 624
BstF5I GGATG 3 cut(s) 634, 884, 1121
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 1 cut(s) 842
BstKTI GATC 5 cut(s) 65, 348, 436, 517, 538
BstMAI GTCTC 2 cut(s) 847, 1071
BstMBI GATC 5 cut(s) 62, 345, 433, 514, 535
BstMWI GCNNNNNNNGC 5 cut(s) 248, 281, 592, 905, 981
BstSFI CTRYAG 3 cut(s) 155, 240, 666
BstV1I GCAGC 4 cut(s) 226, 254, 683, 745
BstV2I GAAGAC 1 cut(s) 541
BstX2I RGATCY 3 cut(s) 62, 433, 514
BstYI RGATCY 3 cut(s) 62, 433, 514
BsuRI GGCC 1 cut(s) 984
BtgI CCRYGG 1 cut(s) 624
BtrI CACGTC 1 cut(s) 1014
BtsCI GGATG 3 cut(s) 634, 884, 1121
BtsIMutI CAGTG 3 cut(s) 181, 558, 901
BveI ACCTGC 1 cut(s) 932
Cac8I GCNNGC 3 cut(s) 159, 282, 354
CaiI CAGNNNCTG 2 cut(s) 518, 677
CciI TCATGA 1 cut(s) 847
CfoI GCGC 1 cut(s) 842
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 1 cut(s) 983
Csp6I GTAC 3 cut(s) 391, 758, 1049
CviAII CATG 3 cut(s) 500, 625, 848
CviQI GTAC 3 cut(s) 391, 758, 1049
DdeI CTNAG 2 cut(s) 636, 1028
DpnI GATC 5 cut(s) 64, 347, 435, 516, 537
DpnII GATC 5 cut(s) 62, 345, 433, 514, 535
Eam1104I CTCTTC 2 cut(s) 187, 930
EarI CTCTTC 2 cut(s) 187, 930
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 3 cut(s) 624, 721, 924
Eco24I GRGCYC 1 cut(s) 102
Eco47III AGCGCT 1 cut(s) 841
Eco53kI GAGCTC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 1036
EcoT14I CCWWGG 3 cut(s) 624, 721, 924
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 3 cut(s) 624, 721, 924
Esp3I CGTCTC 1 cut(s) 847
FaeI CATG 3 cut(s) 503, 628, 851
FaqI GGGAC 1 cut(s) 152
FatI CATG 3 cut(s) 499, 624, 847
FauI CCCGC 1 cut(s) 177
Fnu4HI GCNGC 5 cut(s) 240, 243, 633, 672, 734
FokI GGATG 3 cut(s) 641, 891, 1108
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 5 cut(s) 240, 243, 633, 672, 734
FspBI CTAG 2 cut(s) 804, 959
GlaI GCGC 1 cut(s) 841
GluI GCNGC 5 cut(s) 240, 243, 633, 672, 734
GsuI CTGGAG 1 cut(s) 1119
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 1 cut(s) 984
HapII CCGG 1 cut(s) 285
HhaI GCGC 1 cut(s) 842
Hin1II CATG 3 cut(s) 503, 628, 851
Hin6I GCGC 1 cut(s) 840
HinP1I GCGC 1 cut(s) 840
HindIII AAGCTT 1 cut(s) 973
HinfI GANTC 2 cut(s) 260, 647
HpaII CCGG 1 cut(s) 285
HphI GGTGA 3 cut(s) 125, 158, 220
Hpy166II GTNNAC 2 cut(s) 149, 752
Hpy188I TCNGA 3 cut(s) 216, 702, 1089
Hpy188III TCNNGA 8 cut(s) 140, 257, 268, 419, 518, 539, 604, 848
Hpy8I GTNNAC 2 cut(s) 149, 752
Hpy99I CGWCG 1 cut(s) 1018
HpyAV CCTTC 4 cut(s) 53, 140, 146, 653
HpyCH4III ACNGT 6 cut(s) 146, 176, 562, 679, 749, 896
HpyCH4IV ACGT 2 cut(s) 856, 1013
HpyCH4V TGCA 6 cut(s) 27, 81, 157, 179, 242, 733
HpyF10VI GCNNNNNNNGC 5 cut(s) 248, 281, 592, 905, 981
HpyF3I CTNAG 2 cut(s) 636, 1028
HpySE526I ACGT 2 cut(s) 856, 1013
Hsp92II CATG 3 cut(s) 503, 628, 851
HspAI GCGC 1 cut(s) 840
KpnI GGTACC 1 cut(s) 1052
Kzo9I GATC 5 cut(s) 62, 345, 433, 514, 535
LguI GCTCTTC 1 cut(s) 187
LmnI GCTCC 5 cut(s) 86, 97, 610, 847, 904
Lsp1109I GCAGC 4 cut(s) 226, 254, 683, 745
LweI GCATC 3 cut(s) 195, 437, 619
MaeI CTAG 2 cut(s) 804, 959
MaeII ACGT 2 cut(s) 856, 1013
MaeIII GTNAC 6 cut(s) 140, 290, 427, 508, 679, 799
MalI GATC 5 cut(s) 64, 347, 435, 516, 537
MboI GATC 5 cut(s) 62, 345, 433, 514, 535
MboII GAAGA 9 cut(s) 204, 372, 456, 541, 668, 686, 689, 947, 1030
MfeI CAATTG 1 cut(s) 198
MflI RGATCY 3 cut(s) 62, 433, 514
MhlI GDGCHC 2 cut(s) 102, 901
MluCI AATT 5 cut(s) 52, 198, 742, 771, 1036
MlyI GAGTC 1 cut(s) 656
MmeI TCCRAC 1 cut(s) 90
MseI TTAA 5 cut(s) 480, 566, 575, 978, 1044
MslI CAYNNNNRTG 2 cut(s) 138, 1111
MspA1I CMGCKG 2 cut(s) 239, 245
MspI CCGG 1 cut(s) 285
MunI CAATTG 1 cut(s) 198
MwoI GCNNNNNNNGC 5 cut(s) 248, 281, 592, 905, 981
NcoI CCATGG 1 cut(s) 624
NdeII GATC 5 cut(s) 62, 345, 433, 514, 535
NlaIII CATG 3 cut(s) 503, 628, 851
NlaIV GGNNCC 5 cut(s) 64, 88, 128, 403, 1050
NmuCI GTSAC 2 cut(s) 140, 290
NspV TTCGAA 1 cut(s) 57
OliI CACNNNNGTG 1 cut(s) 138
PagI TCATGA 1 cut(s) 847
PaqCI CACCTGC 1 cut(s) 932
PciSI GCTCTTC 1 cut(s) 187
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 5 cut(s) 241, 244, 634, 673, 735
PleI GAGTC 1 cut(s) 655
PpsI GAGTC 1 cut(s) 655
PshBI ATTAAT 1 cut(s) 575
Psp124BI GAGCTC 1 cut(s) 102
PspN4I GGNNCC 5 cut(s) 64, 88, 128, 403, 1050
PspPI GGNCC 1 cut(s) 983
PsrI GAACNNNNNNTAC 2 cut(s) 468, 500
PstI CTGCAG 2 cut(s) 159, 244
PstNI CAGNNNCTG 2 cut(s) 518, 677
PsuI RGATCY 3 cut(s) 62, 433, 514
PvuII CAGCTG 2 cut(s) 239, 245
RsaI GTAC 3 cut(s) 392, 759, 1050
RsaNI GTAC 3 cut(s) 391, 758, 1049
RseI CAYNNNNRTG 2 cut(s) 138, 1111
SacI GAGCTC 1 cut(s) 102
SapI GCTCTTC 1 cut(s) 187
SaqAI TTAA 5 cut(s) 480, 566, 575, 978, 1044
SatI GCNGC 5 cut(s) 240, 243, 633, 672, 734
Sau3AI GATC 5 cut(s) 62, 345, 433, 514, 535
Sau96I GGNCC 1 cut(s) 983
SchI GAGTC 1 cut(s) 656
SduI GDGCHC 2 cut(s) 102, 901
SfaNI GCATC 3 cut(s) 195, 437, 619
SfcI CTRYAG 3 cut(s) 155, 240, 666
SfuI TTCGAA 1 cut(s) 57
SmiMI CAYNNNNRTG 2 cut(s) 138, 1111
SmlI CTYRAG 2 cut(s) 417, 461
SmoI CTYRAG 2 cut(s) 417, 461
SpeI ACTAGT 1 cut(s) 803
Sse9I AATT 5 cut(s) 52, 198, 742, 771, 1036
SsiI CCGC 3 cut(s) 161, 170, 632
SspMI CTAG 2 cut(s) 804, 959
SstI GAGCTC 1 cut(s) 102
StyI CCWWGG 3 cut(s) 624, 721, 924
TaaI ACNGT 6 cut(s) 146, 176, 562, 679, 749, 896
TaiI ACGT 2 cut(s) 859, 1016
TaqI TCGA 4 cut(s) 57, 256, 1006, 1016
TasI AATT 5 cut(s) 52, 198, 742, 771, 1036
TatI WGTACW 1 cut(s) 757
TauI GCSGC 1 cut(s) 635
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 5 cut(s) 480, 566, 575, 978, 1044
Tru9I TTAA 5 cut(s) 480, 566, 575, 978, 1044
TscAI CASTG 3 cut(s) 181, 565, 901
TseFI GTSAC 2 cut(s) 140, 290
TseI GCWGC 4 cut(s) 239, 242, 671, 733
Tsp45I GTSAC 2 cut(s) 140, 290
TspDTI ATGAA 4 cut(s) 488, 541, 814, 1029
TspGWI ACGGA 1 cut(s) 1059
TspRI CASTG 3 cut(s) 181, 565, 901
VspI ATTAAT 1 cut(s) 575
XapI RAATTY 3 cut(s) 52, 771, 1036
XspI CTAG 2 cut(s) 804, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.