Rorug02G0270300

Dihydroorotase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010110
Physical Location & Seq
Reverse (-)
28655777 .. 28658442
2666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0270300.1

Sequence Viewer

Length: 798 bp
ATGGCCACTATGCTTGTCCAACCACCAATCCCGGCTTTCCATGCTAACATTCCTCCAATGCTCAGAACCACAAACATGGTGATAACTCCCACTTCACCCTCAACCACTCATCAAAAAACTCAAAGCTTAAGATTTGGTGAGTGTCCACCTGGGTTTAGGCGCATTTACAGAGTCAAAGTTGCAGAACAAACCTCTGGCCTAGTTGGGGATGAGACACCCACCCAGATTAAAACTGAGCTTCACCAGGCACTCCAAGGTATTAATAGAGGGATATTTGGAGTCCCATCTGCAAAGAAATCTGAGATTGAAGGTCTGGTTAAGCAGCTGGAGTCTCAGAATCCAACTCCAGATCCTACTGTGAATTTAGAAAAGGTGGGTGGCTGCTGGAAACTTGTGTATAGCACAATTACAATTTTAGGCTCAAGAAGAACAAAGCTGGGATTGAGGGACTTCATCTCATTGGGAGATTTCTACCAGAATATTGATATTGCTAAGGGCAATGCAGTCAATGTGATCAAGTTCGATGTGAGAGGATTGAATCTCTTTAATGGACGGCTAACAATTGTGGCCTCCTTCAAGAAAGCATCCAAATCAAGAGTTGACATCAGCTATGACAACTCTACAATCACTCCGGTTCAGTTGATGAATGTGTTTCGGAAAAATTATGATATTCTGCTTGGAATCTTCAATCCAGACGGTTGGCTCGAGATCACATATGTTGATGATACCATGAGGATAGGAAGGGATGACAAAGGCAATATCTTCATATTAGAGAGATCAGAAGAAAATTTGATTTAA

Protein Analysis

265

Amino Acids

29.61

Weight (kDa)

9.3

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 75 - 257 1.9e-33 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22930 AT4G22930
fragaria_vesca FvH4_6g26410 FvH4_6g26410 FvH4_6g26410 FvH4_6g26410
malus_domestica MD09G1233100.v1.1
prunus_persica Prupe.3G131200_v2.0.a1
pyrus_communis pycom09g16330
rosa_chinensis RchiOBHm_Chr2g0127911
rosa_laevigata RLG00000018930
rosa_multiflora Rmu_ssc0000093.1_g000008
rosa_roxburghii Rroxscaffold_2G00116850
rosa_rugosa Rorug02G0270300 Rorug02G0270300 Rorug02G0270400
rosa_samantha Rh2AG322800 Rh2BG331700 Rh2CG310800 Rh2DG350300
rosa_wichuraiana Rw2G026180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 344
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 361, 787
AfiI CCNNNNNNNGG 1 cut(s) 205
AflII CTTAAG 1 cut(s) 127
AgsI TTSAA 4 cut(s) 308, 538, 577, 688
AjnI CCWGG 2 cut(s) 148, 243
AluBI AGCT 5 cut(s) 126, 238, 325, 436, 609
AluI AGCT 5 cut(s) 126, 238, 325, 436, 609
Alw26I GTCTC 2 cut(s) 206, 336
AlwI GGATC 1 cut(s) 344
Ama87I CYCGRG 1 cut(s) 704
AoxI GGCC 3 cut(s) 3, 196, 567
ApeKI GCWGC 2 cut(s) 322, 381
ApoI RAATTY 2 cut(s) 361, 787
AseI ATTAAT 1 cut(s) 261
AspLEI GCGC 1 cut(s) 162
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 4 cut(s) 87, 91, 149, 233
AvaI CYCGRG 1 cut(s) 704
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 2 cut(s) 334, 368
BccI CCATC 1 cut(s) 292
BceAI ACGGC 1 cut(s) 569
BciT130I CCWGG 2 cut(s) 150, 245
BclI TGATCA 1 cut(s) 513
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 206, 336
BfaI CTAG 1 cut(s) 200
BfrI CTTAAG 1 cut(s) 127
BisI GCNGC 2 cut(s) 323, 382
BlsI GCNGC 2 cut(s) 324, 383
Bme1390I CCNGG 3 cut(s) 32, 150, 245
BmeT110I CYCGRG 1 cut(s) 704
BmrFI CCNGG 3 cut(s) 32, 150, 245
BmsI GCATC 1 cut(s) 593
BpmI CTGGAG 2 cut(s) 330, 347
Bpu10I CCTNAGC 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 149, 253
BsaWI WCCGGW 1 cut(s) 631
BsaXI ACNNNNNCTCC 2 cut(s) 613, 643
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse3DI GCAATG 1 cut(s) 505
BseBI CCWGG 2 cut(s) 150, 245
BseDI CCNNGG 2 cut(s) 149, 253
BseGI GGATG 3 cut(s) 214, 584, 751
BseLI CCNNNNNNNGG 1 cut(s) 205
BseMI GCAATG 1 cut(s) 505
BseMII CTCAG 4 cut(s) 76, 225, 291, 347
BseXI GCAGC 2 cut(s) 334, 368
BseYI CCCAGC 1 cut(s) 436
BshFI GGCC 3 cut(s) 5, 198, 569
BsiHKCI CYCGRG 1 cut(s) 704
BsiSI CCGG 2 cut(s) 32, 632
BslFI GGGAC 2 cut(s) 266, 461
BslI CCNNNNNNNGG 1 cut(s) 205
BsmAI GTCTC 2 cut(s) 206, 336
BsmFI GGGAC 2 cut(s) 266, 461
BsnI GGCC 3 cut(s) 5, 198, 569
BsoBI CYCGRG 1 cut(s) 704
Bsp143I GATC 4 cut(s) 349, 513, 708, 776
BspANI GGCC 3 cut(s) 5, 198, 569
BspCNI CTCAG 4 cut(s) 75, 226, 292, 346
BspPI GGATC 1 cut(s) 344
BspTI CTTAAG 1 cut(s) 127
BsrDI GCAATG 1 cut(s) 505
BssECI CCNNGG 2 cut(s) 149, 253
BssMI GATC 4 cut(s) 349, 513, 708, 776
BssT1I CCWWGG 1 cut(s) 253
Bst2UI CCWGG 2 cut(s) 150, 245
Bst4CI ACNGT 2 cut(s) 358, 698
BstAFI CTTAAG 1 cut(s) 127
BstDEI CTNAG 5 cut(s) 62, 234, 300, 333, 492
BstF5I GGATG 3 cut(s) 214, 584, 751
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 4 cut(s) 352, 516, 711, 779
BstMAI GTCTC 2 cut(s) 206, 336
BstMBI GATC 4 cut(s) 349, 513, 708, 776
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstNI CCWGG 2 cut(s) 150, 245
BstSCI CCNGG 3 cut(s) 30, 148, 243
BstV1I GCAGC 2 cut(s) 334, 368
BstX2I RGATCY 1 cut(s) 349
BstXI CCANNNNNNTGG 2 cut(s) 76, 699
BstYI RGATCY 1 cut(s) 349
BsuRI GGCC 3 cut(s) 5, 198, 569
BtsCI GGATG 3 cut(s) 214, 584, 751
CfoI GCGC 1 cut(s) 162
CspCI CAANNNNNGTGG 1 cut(s) 30
CviAII CATG 3 cut(s) 41, 76, 730
DdeI CTNAG 5 cut(s) 62, 234, 300, 333, 492
DpnI GATC 4 cut(s) 351, 515, 710, 778
DpnII GATC 4 cut(s) 349, 513, 708, 776
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 253
Eco88I CYCGRG 1 cut(s) 704
EcoRII CCWGG 2 cut(s) 148, 243
EcoT14I CCWWGG 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 253
FaeI CATG 3 cut(s) 44, 79, 733
FaqI GGGAC 2 cut(s) 266, 461
FatI CATG 3 cut(s) 40, 75, 729
FauNDI CATATG 1 cut(s) 715
FbaI TGATCA 1 cut(s) 513
Fnu4HI GCNGC 2 cut(s) 323, 382
FokI GGATG 3 cut(s) 221, 571, 758
Fsp4HI GCNGC 2 cut(s) 323, 382
FspBI CTAG 1 cut(s) 200
GlaI GCGC 1 cut(s) 161
GluI GCNGC 2 cut(s) 323, 382
GsaI CCCAGC 1 cut(s) 440
GsuI CTGGAG 2 cut(s) 330, 347
HaeIII GGCC 3 cut(s) 5, 198, 569
HapII CCGG 2 cut(s) 32, 632
HhaI GCGC 1 cut(s) 162
Hin1II CATG 3 cut(s) 44, 79, 733
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 124
HinfI GANTC 6 cut(s) 171, 279, 329, 337, 538, 681
HpaII CCGG 2 cut(s) 32, 632
HphI GGTGA 4 cut(s) 87, 91, 149, 233
Hpy166II GTNNAC 2 cut(s) 146, 601
Hpy188I TCNGA 5 cut(s) 65, 301, 336, 657, 781
Hpy188III TCNNGA 6 cut(s) 347, 423, 577, 594, 692, 706
Hpy8I GTNNAC 2 cut(s) 146, 601
HpyAV CCTTC 3 cut(s) 302, 583, 735
HpyCH4III ACNGT 2 cut(s) 358, 698
HpyCH4V TGCA 3 cut(s) 182, 290, 503
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 5 cut(s) 62, 234, 300, 333, 492
Hsp92II CATG 3 cut(s) 44, 79, 733
HspAI GCGC 1 cut(s) 160
Ksp22I TGATCA 1 cut(s) 513
Kzo9I GATC 4 cut(s) 349, 513, 708, 776
Lsp1109I GCAGC 2 cut(s) 334, 368
LweI GCATC 1 cut(s) 593
MaeI CTAG 1 cut(s) 200
MalI GATC 4 cut(s) 351, 515, 710, 778
MboI GATC 4 cut(s) 349, 513, 708, 776
MboII GAAGA 4 cut(s) 438, 676, 754, 794
MfeI CAATTG 1 cut(s) 561
MflI RGATCY 1 cut(s) 349
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 361, 405, 411, 561, 661, 787
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 180, 288, 338
MmeI TCCRAC 2 cut(s) 43, 365
MnlI CCTC 8 cut(s) 63, 109, 202, 260, 438, 524, 580, 726
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 128, 228, 261, 318, 546, 796
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 325
MspCI CTTAAG 1 cut(s) 127
MspI CCGG 2 cut(s) 32, 632
MspR9I CCNGG 3 cut(s) 32, 150, 245
MunI CAATTG 1 cut(s) 561
MvaI CCWGG 2 cut(s) 150, 245
MwoI GCNNNNNNNGC 1 cut(s) 41
NciI CCSGG 1 cut(s) 32
NdeI CATATG 1 cut(s) 715
NdeII GATC 4 cut(s) 349, 513, 708, 776
NlaIII CATG 3 cut(s) 44, 79, 733
PaeR7I CTCGAG 1 cut(s) 704
PcsI WCGNNNNNNNCGW 1 cut(s) 702
PfeI GAWTC 3 cut(s) 337, 538, 681
PkrI GCNGC 2 cut(s) 324, 383
PleI GAGTC 3 cut(s) 179, 287, 337
PpsI GAGTC 3 cut(s) 179, 287, 337
PshBI ATTAAT 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 148, 243
PspFI CCCAGC 1 cut(s) 436
PspGI CCWGG 2 cut(s) 148, 243
PsuI RGATCY 1 cut(s) 349
PvuII CAGCTG 1 cut(s) 325
RseI CAYNNNNRTG 1 cut(s) 74
SaqAI TTAA 6 cut(s) 128, 228, 261, 318, 546, 796
SatI GCNGC 2 cut(s) 323, 382
Sau3AI GATC 4 cut(s) 349, 513, 708, 776
SchI GAGTC 3 cut(s) 180, 288, 338
ScrFI CCNGG 3 cut(s) 32, 150, 245
SfaNI GCATC 1 cut(s) 593
Sfr274I CTCGAG 1 cut(s) 704
SlaI CTCGAG 1 cut(s) 704
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 3 cut(s) 127, 421, 704
SmoI CTYRAG 3 cut(s) 127, 421, 704
Sse9I AATT 6 cut(s) 361, 405, 411, 561, 661, 787
SspI AATATT 1 cut(s) 481
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 3 cut(s) 30, 148, 243
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 2 cut(s) 358, 698
TaqI TCGA 2 cut(s) 522, 705
TasI AATT 6 cut(s) 361, 405, 411, 561, 661, 787
TfiI GAWTC 3 cut(s) 337, 538, 681
Tru1I TTAA 6 cut(s) 128, 228, 261, 318, 546, 796
Tru9I TTAA 6 cut(s) 128, 228, 261, 318, 546, 796
TseI GCWGC 2 cut(s) 322, 381
TspDTI ATGAA 3 cut(s) 442, 659, 754
Vha464I CTTAAG 1 cut(s) 127
VspI ATTAAT 1 cut(s) 261
XapI RAATTY 2 cut(s) 361, 787
XhoI CTCGAG 1 cut(s) 704
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.