MD09G1279600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014601
Physical Location & Seq
Reverse (-)
35649637 .. 35650746
1110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1279600.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGATAGGAGCTGTGCCATTCGGGGTGCTGGCTGCGTGCGTCGTCGTTTTGGTCCCAATGGCCATGGCCGGATACCATCTCAGCCGCAAAAAGATGCTTTTCTTCAGCGGCACTCTCTTCATCGCTCTCGCCATCGGCGTCCACCTCAGCCCTTACTTCCCCTCCGTCAACGACTTCGTCACCACGGTCTCCTCCGTCGTCGTCTTCGAGAACCGCCGCGACTCCTGCCTCTCCCACCTCCACGACGTTGTTTGGGAGGTTACTCCTTCCCCCAATTTCAACGCCCTCGACAGCAACACCGTCAATTACGACAAGTCCTGGGCTTGGCCTTCTTCCTTCGCCGTTTCTGCATGTGGATTTCAGAAGCTGGGTCGTTTCGACGCCTCCGATCTGCTCAACGGCTCGTGGGTCGTGGTGGCCGGAGACTCTCAGGCGAGGCTGGTGGCGCTTTCGCTGCTCAGCCTGGTTCTGGATTCTGGGCCGCTGGAGAAGGTTAACAAGGATCTATTCAAGAGGCACAGTGATTATCAGATTGTGGTGGGCGAGATTGGGATGCGATTGGATTTCATCTGGGCTCCTTATACTGTGAATTTGACCAATCTGGTGTCGAAGTTCAAGCGAAATCGGAACTTCCCGGATGTGTTGGTGATGGGTTCTGGGCTATGGCACATGCTGCACTTCACCAACGCTTCAGATTTCCGTGTTCAACTGCAATCACTCAGGAGATCTGCGGCGTCCTTGTTGCCAGTTTCGCCGGCGATTGGCTCTGAAAAGCCGGAAACCAGCTCGGCTTCAGGCTCTGGTTCGGCCCGGACGCCACACTTGTTCTGGGTTGGCATGCCCACGTTGATAAACTCGATGTTGAATACGGAGGAGAAGAGGGAGAAGATGACTGATGCAATGCGGGGTGAGTATGATAAGGAGCTTCAAAAGAGTAAGCTTTTGCGGAAGTCCGGCGGTCCGCTTTGGTTACTGGACATTGAGTCCCTGAGTTGGAATTGCGGGGTTCGATGTACGGTTGATGGAATGCATTATGATATGCGTGTTTACGAAGCAGTGCTGCACATCATGCTAAATGCATTGCTCATCGAGTCTCACCAGAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

370

Amino Acids

40.99

Weight (kDa)

8.22

Isoelectric Point (pI)

49.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014601)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03210
fragaria_vesca FvH4_1g27360
malus_domestica MD09G1279600.v1.1
prunus_persica Prupe.3G014700_v2.0.a1
pyrus_communis pycom09g18770
rosa_chinensis RchiOBHm_Chr3g0488741
rosa_laevigata RLG00000022925
rosa_multiflora Rmu_sc0000265.1_g000016 Rmu_sc0001208.1_g000071 Rmu_sc0001208.1_g000072
rosa_roxburghii Rroxscaffold_6G00393910
rosa_rugosa Rorug03G0241200
rosa_samantha Rh3AG289500 Rh3BG326500 Rh3CG324000 Rh3DG322400
rosa_wichuraiana Rw3G025630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 219
AclWI GGATC 1 cut(s) 510
AcoI YGGCCR 3 cut(s) 60, 66, 417
AcsI RAATTY 1 cut(s) 589
AcuI CTGAAG 3 cut(s) 88, 675, 777
AcyI GRCGYC 4 cut(s) 138, 381, 734, 815
AfaI GTAC 1 cut(s) 1015
AfiI CCNNNNNNNGG 4 cut(s) 324, 469, 761, 993
AgsI TTSAA 6 cut(s) 280, 511, 616, 707, 865, 929
AhdI GACNNNNNGTC 1 cut(s) 982
AjnI CCWGG 2 cut(s) 317, 462
AluBI AGCT 6 cut(s) 11, 367, 786, 925, 940, 1105
AluI AGCT 6 cut(s) 11, 367, 786, 925, 940, 1105
Alw26I GTCTC 3 cut(s) 193, 417, 1098
AlwI GGATC 1 cut(s) 510
AlwNI CAGNNNCTG 2 cut(s) 367, 800
AoxI GGCC 6 cut(s) 60, 66, 326, 417, 479, 807
ApeKI GCWGC 4 cut(s) 32, 454, 673, 1060
ApoI RAATTY 1 cut(s) 589
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 4 cut(s) 52, 479, 808, 959
AsuC2I CCSGG 2 cut(s) 635, 811
AsuHPI GGTGA 5 cut(s) 172, 658, 673, 920, 1088
AvaII GGWCC 2 cut(s) 52, 959
BalI TGGCCA 1 cut(s) 62
BanII GRGCYC 1 cut(s) 577
BauI CACGAG 1 cut(s) 403
BbsI GAAGAC 1 cut(s) 196
BbvCI CCTCAGC 1 cut(s) 146
BbvI GCAGC 4 cut(s) 19, 441, 660, 1047
BccI CCATC 4 cut(s) 84, 140, 643, 1016
BceAI ACGGC 2 cut(s) 326, 415
BciT130I CCWGG 2 cut(s) 319, 464
BciVI GTATCC 1 cut(s) 65
BcnI CCSGG 2 cut(s) 635, 811
BcoDI GTCTC 3 cut(s) 193, 417, 1098
BfoI RGCGCY 1 cut(s) 449
BfuI GTATCC 1 cut(s) 65
BglII AGATCT 1 cut(s) 725
BisI GCNGC 9 cut(s) 33, 85, 109, 217, 455, 482, 674, 732, 1061
BlpI GCTNAGC 1 cut(s) 458
BlsI GCNGC 9 cut(s) 34, 86, 110, 218, 456, 483, 675, 733, 1062
Bme1390I CCNGG 4 cut(s) 319, 464, 635, 811
Bme18I GGWCC 2 cut(s) 52, 959
BmeRI GACNNNNNGTC 1 cut(s) 982
BmgT120I GGNCC 4 cut(s) 52, 479, 808, 959
BmiI GGNNCC 2 cut(s) 54, 576
BmrFI CCNGG 4 cut(s) 319, 464, 635, 811
BmsI GCATC 3 cut(s) 84, 543, 886
BpiI GAAGAC 1 cut(s) 196
BpmI CTGGAG 1 cut(s) 506
Bpu10I CCTNAGC 1 cut(s) 146
Bpu1102I GCTNAGC 1 cut(s) 458
BpuMI CCSGG 2 cut(s) 635, 811
BsaHI GRCGYC 4 cut(s) 138, 381, 734, 815
BsaI GGTCTC 1 cut(s) 193
BsaJI CCNNGG 3 cut(s) 63, 183, 318
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 4 cut(s) 324, 469, 761, 993
Bse118I RCCGGY 1 cut(s) 754
Bse1I ACTGG 2 cut(s) 746, 978
Bse3DI GCAATG 2 cut(s) 906, 1079
BseBI CCWGG 2 cut(s) 319, 464
BseDI CCNNGG 3 cut(s) 63, 183, 318
BseGI GGATG 2 cut(s) 558, 643
BseLI CCNNNNNNNGG 4 cut(s) 324, 469, 761, 993
BseMI GCAATG 2 cut(s) 906, 1079
BseMII CTCAG 6 cut(s) 94, 160, 443, 472, 733, 980
BseNI ACTGG 2 cut(s) 746, 978
BseRI GAGGAG 2 cut(s) 181, 887
BseXI GCAGC 4 cut(s) 19, 441, 660, 1047
BseYI CCCAGC 1 cut(s) 367
BsgI GTGCAG 2 cut(s) 659, 1046
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 6 cut(s) 62, 68, 328, 419, 481, 809
BsiSI CCGG 7 cut(s) 69, 420, 635, 755, 776, 811, 954
BslFI GGGAC 2 cut(s) 38, 970
BslI CCNNNNNNNGG 4 cut(s) 324, 469, 761, 993
BsmAI GTCTC 3 cut(s) 193, 417, 1098
BsmFI GGGAC 2 cut(s) 38, 970
BsmI GAATGC 1 cut(s) 1032
BsnI GGCC 6 cut(s) 62, 68, 328, 419, 481, 809
Bso31I GGTCTC 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 577
Bsp143I GATC 3 cut(s) 388, 502, 725
Bsp1720I GCTNAGC 1 cut(s) 458
Bsp19I CCATGG 1 cut(s) 63
BspANI GGCC 6 cut(s) 62, 68, 328, 419, 481, 809
BspCNI CTCAG 6 cut(s) 93, 159, 442, 471, 732, 981
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 2 cut(s) 54, 576
BspPI GGATC 1 cut(s) 510
BspTNI GGTCTC 1 cut(s) 193
BsrDI GCAATG 2 cut(s) 906, 1079
BsrFI RCCGGY 1 cut(s) 754
BsrI ACTGG 2 cut(s) 746, 978
BssAI RCCGGY 1 cut(s) 754
BssECI CCNNGG 3 cut(s) 63, 183, 318
BssMI GATC 3 cut(s) 388, 502, 725
BssNI GRCGYC 4 cut(s) 138, 381, 734, 815
BssSI CACGAG 1 cut(s) 403
BssT1I CCWWGG 1 cut(s) 63
Bst2BI CACGAG 1 cut(s) 403
Bst2UI CCWGG 2 cut(s) 319, 464
Bst4CI ACNGT 5 cut(s) 187, 301, 521, 586, 1018
Bst6I CTCTTC 2 cut(s) 122, 872
BstACI GRCGYC 4 cut(s) 138, 381, 734, 815
BstAPI GCANNNNNTGC 2 cut(s) 673, 1069
BstC8I GCNNGC 4 cut(s) 30, 37, 756, 839
BstDEI CTNAG 6 cut(s) 80, 146, 429, 458, 719, 989
BstDSI CCRYGG 2 cut(s) 63, 183
BstF5I GGATG 2 cut(s) 558, 643
BstFNI CGCG 1 cut(s) 219
BstH2I RGCGCY 1 cut(s) 449
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 3 cut(s) 391, 505, 728
BstMAI GTCTC 3 cut(s) 193, 417, 1098
BstMBI GATC 3 cut(s) 388, 502, 725
BstMWI GCNNNNNNNGC 7 cut(s) 225, 347, 445, 454, 673, 751, 1069
BstNI CCWGG 2 cut(s) 319, 464
BstNSI RCATGY 3 cut(s) 354, 673, 841
BstSCI CCNGG 4 cut(s) 317, 462, 633, 809
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 4 cut(s) 19, 441, 660, 1047
BstV2I GAAGAC 1 cut(s) 196
BstX2I RGATCY 2 cut(s) 502, 725
BstYI RGATCY 2 cut(s) 502, 725
BsuI GTATCC 1 cut(s) 65
BsuRI GGCC 6 cut(s) 62, 68, 328, 419, 481, 809
BtgI CCRYGG 2 cut(s) 63, 183
BtgZI GCGATG 1 cut(s) 106
BtsCI GGATG 2 cut(s) 558, 643
BtsI GCAGTG 1 cut(s) 1062
BtsIMutI CAGTG 2 cut(s) 526, 1062
Cac8I GCNNGC 4 cut(s) 30, 37, 756, 839
CaiI CAGNNNCTG 2 cut(s) 367, 800
CfoI GCGC 1 cut(s) 448
Cfr10I RCCGGY 1 cut(s) 754
Cfr13I GGNCC 4 cut(s) 52, 479, 808, 959
CpoI CGGWCCG 1 cut(s) 959
CseI GACGC 5 cut(s) 28, 127, 389, 723, 823
Csp6I GTAC 1 cut(s) 1014
CspI CGGWCCG 1 cut(s) 959
CviAII CATG 5 cut(s) 64, 351, 670, 838, 1069
CviQI GTAC 1 cut(s) 1014
DdeI CTNAG 6 cut(s) 80, 146, 429, 458, 719, 989
DpnI GATC 3 cut(s) 390, 504, 727
DpnII GATC 3 cut(s) 388, 502, 725
DriI GACNNNNNGTC 1 cut(s) 982
EaeI YGGCCR 3 cut(s) 60, 66, 417
Eam1104I CTCTTC 2 cut(s) 122, 872
Eam1105I GACNNNNNGTC 1 cut(s) 982
EarI CTCTTC 2 cut(s) 122, 872
Eco130I CCWWGG 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 577
Eco31I GGTCTC 1 cut(s) 193
Eco47I GGWCC 2 cut(s) 52, 959
Eco57I CTGAAG 3 cut(s) 88, 675, 777
EcoRII CCWGG 2 cut(s) 317, 462
EcoT14I CCWWGG 1 cut(s) 63
EcoT22I ATGCAT 2 cut(s) 1032, 1081
EcoT38I GRGCYC 1 cut(s) 577
ErhI CCWWGG 1 cut(s) 63
FaeI CATG 5 cut(s) 67, 354, 673, 841, 1072
FaqI GGGAC 2 cut(s) 38, 970
FatI CATG 5 cut(s) 63, 350, 669, 837, 1068
FauI CCCGC 2 cut(s) 897, 995
Fnu4HI GCNGC 9 cut(s) 33, 85, 109, 217, 455, 482, 674, 732, 1061
FokI GGATG 2 cut(s) 565, 650
FriOI GRGCYC 1 cut(s) 577
Fsp4HI GCNGC 9 cut(s) 33, 85, 109, 217, 455, 482, 674, 732, 1061
GlaI GCGC 1 cut(s) 447
GluI GCNGC 9 cut(s) 33, 85, 109, 217, 455, 482, 674, 732, 1061
GsaI CCCAGC 1 cut(s) 371
GsuI CTGGAG 1 cut(s) 506
HaeII RGCGCY 1 cut(s) 449
HaeIII GGCC 6 cut(s) 62, 68, 328, 419, 481, 809
HapII CCGG 7 cut(s) 69, 420, 635, 755, 776, 811, 954
HgaI GACGC 5 cut(s) 28, 127, 389, 723, 823
HhaI GCGC 1 cut(s) 448
Hin1I GRCGYC 4 cut(s) 138, 381, 734, 815
Hin1II CATG 5 cut(s) 67, 354, 673, 841, 1072
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HincII GTYRAC 2 cut(s) 169, 496
HindII GTYRAC 2 cut(s) 169, 496
HindIII AAGCTT 2 cut(s) 938, 1103
HinfI GANTC 5 cut(s) 221, 425, 473, 983, 1091
HpaI GTTAAC 1 cut(s) 496
HpaII CCGG 7 cut(s) 69, 420, 635, 755, 776, 811, 954
HphI GGTGA 5 cut(s) 172, 658, 673, 920, 1088
Hpy166II GTNNAC 4 cut(s) 142, 169, 496, 1048
Hpy188I TCNGA 6 cut(s) 363, 388, 531, 627, 694, 769
Hpy188III TCNNGA 4 cut(s) 208, 470, 511, 721
Hpy8I GTNNAC 4 cut(s) 142, 169, 496, 1048
Hpy99I CGWCG 6 cut(s) 44, 47, 200, 203, 248, 383
HpyAV CCTTC 4 cut(s) 276, 339, 346, 484
HpyCH4III ACNGT 5 cut(s) 187, 301, 521, 586, 1018
HpyCH4IV ACGT 2 cut(s) 246, 845
HpyCH4V TGCA 7 cut(s) 350, 676, 712, 899, 1030, 1063, 1079
HpyF10VI GCNNNNNNNGC 7 cut(s) 225, 347, 445, 454, 673, 751, 1069
HpyF3I CTNAG 6 cut(s) 80, 146, 429, 458, 719, 989
HpySE526I ACGT 2 cut(s) 246, 845
Hsp92I GRCGYC 4 cut(s) 138, 381, 734, 815
Hsp92II CATG 5 cut(s) 67, 354, 673, 841, 1072
HspAI GCGC 1 cut(s) 446
KroI GCCGGC 1 cut(s) 754
KroNI GCCGGC 1 cut(s) 756
KspAI GTTAAC 1 cut(s) 496
Kzo9I GATC 3 cut(s) 388, 502, 725
LmnI GCTCC 3 cut(s) 8, 580, 922
Lsp1109I GCAGC 4 cut(s) 19, 441, 660, 1047
LweI GCATC 3 cut(s) 84, 543, 886
MaeII ACGT 2 cut(s) 246, 845
MaeIII GTNAC 3 cut(s) 178, 259, 969
MalI GATC 3 cut(s) 390, 504, 727
MboI GATC 3 cut(s) 388, 502, 725
MboII GAAGA 6 cut(s) 94, 109, 196, 324, 889, 898
MflI RGATCY 2 cut(s) 502, 725
MhlI GDGCHC 1 cut(s) 577
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 4 cut(s) 274, 304, 589, 997
MluNI TGGCCA 1 cut(s) 62
MlyI GAGTC 4 cut(s) 215, 419, 992, 1100
MmeI TCCRAC 1 cut(s) 974
Mox20I TGGCCA 1 cut(s) 62
Mph1103I ATGCAT 2 cut(s) 1032, 1081
MreI CGCCGGCG 1 cut(s) 754
MroNI GCCGGC 1 cut(s) 754
MscI TGGCCA 1 cut(s) 62
MseI TTAA 1 cut(s) 495
Msp20I TGGCCA 1 cut(s) 62
MspA1I CMGCKG 2 cut(s) 108, 484
MspI CCGG 7 cut(s) 69, 420, 635, 755, 776, 811, 954
MspR9I CCNGG 4 cut(s) 319, 464, 635, 811
Mva1269I GAATGC 1 cut(s) 1032
MvaI CCWGG 2 cut(s) 319, 464
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 7 cut(s) 225, 347, 445, 454, 673, 751, 1069
NaeI GCCGGC 1 cut(s) 756
NciI CCSGG 2 cut(s) 635, 811
NcoI CCATGG 1 cut(s) 63
NdeII GATC 3 cut(s) 388, 502, 725
NgoMIV GCCGGC 1 cut(s) 754
NlaIII CATG 5 cut(s) 67, 354, 673, 841, 1072
NlaIV GGNNCC 2 cut(s) 54, 576
NmeAIII GCCGAG 1 cut(s) 767
NmuCI GTSAC 1 cut(s) 178
NsiI ATGCAT 2 cut(s) 1032, 1081
NspI RCATGY 3 cut(s) 354, 673, 841
PaeI GCATGC 1 cut(s) 841
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 204, 384
PctI GAATGC 1 cut(s) 1032
PdiI GCCGGC 1 cut(s) 756
PfeI GAWTC 1 cut(s) 473
PflFI GACNNNGTC 1 cut(s) 176
PfoI TCCNGGA 1 cut(s) 633
PkrI GCNGC 9 cut(s) 34, 86, 110, 218, 456, 483, 675, 733, 1062
PleI GAGTC 4 cut(s) 215, 419, 991, 1099
PpsI GAGTC 4 cut(s) 215, 419, 991, 1099
Psp6I CCWGG 2 cut(s) 317, 462
PspFI CCCAGC 1 cut(s) 367
PspGI CCWGG 2 cut(s) 317, 462
PspN4I GGNNCC 2 cut(s) 54, 576
PspPI GGNCC 4 cut(s) 52, 479, 808, 959
PstNI CAGNNNCTG 2 cut(s) 367, 800
PsuI RGATCY 2 cut(s) 502, 725
PsyI GACNNNGTC 1 cut(s) 176
RsaI GTAC 1 cut(s) 1015
RsaNI GTAC 1 cut(s) 1014
Rsr2I CGGWCCG 1 cut(s) 959
RsrII CGGWCCG 1 cut(s) 959
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 9 cut(s) 33, 85, 109, 217, 455, 482, 674, 732, 1061
Sau3AI GATC 3 cut(s) 388, 502, 725
Sau96I GGNCC 4 cut(s) 52, 479, 808, 959
SchI GAGTC 4 cut(s) 215, 419, 992, 1100
ScrFI CCNGG 4 cut(s) 319, 464, 635, 811
SduI GDGCHC 1 cut(s) 577
SfaNI GCATC 3 cut(s) 84, 543, 886
SgrAI CRCCGGYG 1 cut(s) 754
SinI GGWCC 2 cut(s) 52, 959
SphI GCATGC 1 cut(s) 841
Sse9I AATT 4 cut(s) 274, 304, 589, 997
StyD4I CCNGG 4 cut(s) 317, 462, 633, 809
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 5 cut(s) 187, 301, 521, 586, 1018
TaiI ACGT 2 cut(s) 249, 848
TaqI TCGA 7 cut(s) 207, 288, 378, 608, 857, 1009, 1089
TasI AATT 4 cut(s) 274, 304, 589, 997
TauI GCSGC 5 cut(s) 87, 111, 219, 484, 734
TfiI GAWTC 1 cut(s) 473
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TscAI CASTG 2 cut(s) 526, 1062
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 4 cut(s) 32, 454, 673, 1060
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 2 cut(s) 109, 556
TspGWI ACGGA 4 cut(s) 154, 184, 689, 884
TspRI CASTG 2 cut(s) 526, 1062
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 2 cut(s) 52, 959
XapI RAATTY 1 cut(s) 589
XceI RCATGY 3 cut(s) 354, 673, 841
XcmI CCANNNNNNNNNTGG 1 cut(s) 825
Zsp2I ATGCAT 2 cut(s) 1032, 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.