Rorug03G0241200

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0014601
Physical Location & Seq
Forward (+)
22520609 .. 22524204
3596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0241200.1

Sequence Viewer

Length: 1134 bp
ATGGCAGAATCCGAGGATATTCAGCCCCTTGTTTGTGACAATGGTACCGGAATGGTCAAGGCAGGATTTGCTGGAGATGATGCTCCAAGAGCTGTGTTTCCCAGCATTGTAGGGCGCCCTCGCCACACGGGTGTGATGGTTGGGATGGGACAGAAGGACGCCTATGTGGGGGATGAGGCTCAATCGAAGAGAGGTATTCTGACTTTGAAATACCCGATCGAGCATGGTATTGTTAGCAATTGGGATGATATGGAGAAGATCTGGCATCACACATTCTACAATGAGCTCCGTGTGGCTCCAGAAGAGCATCCAGTTCTCCTCACAGAAGCACCCCTTAACCCCAAGGCCAACCGTGAGAAAATGACTCAAATTATGTTTGAGACATTCAACACTCCTGCTATGTACGTTGCAATCCAGGCCGTCCTTTCCCTGTATGCTAGTGGACGTACAACTGGTATTGTTCTTGACTCTGGGGATGGTGTGAGTCACACAGTTCCGATATATGAAGGGTATGCCCTCCCACATGCCATCCTTCGTTTGGACCTGGCAGGTCGTGATCTTACAGATGCCCTCATGAAAATCTTGACTGAACGTGGTTACTCTTTCACCACTACTGCTGAGCGGGAAATTGTGAGAGACATGAAGGAGAAATTAGCCTATATTGCTCTAGACTATGAACAAGAACTAGAGACAGCAAAGACCAGCTCATCTGTTGAGAAGAGTTATGAATTACCTGATGGGCAGGTTATCACGATTGGTGCTGAAAGATTCCGATGCCCTGAAGTCCTCTTCCAGCCATCCATGATCGGGATGGAAGCTGCTGGTATCCATGAAACCACATACAACTCTATCATGAAGTGTGATGTTGATATCAGGAAGGATCTCTATGGCAACATTGTTCTTTCCGGAGGCTCCACTATGTTCCCTGGAATTGCTGATAGGATGAGCAAAGAGATTACAGCATTAGCTCCAAGTAGCATGAAAATCAAGGTCGTAGCACCGCCCGAGAGGAAATACAGTGTCTGGATAGGAGGCTCCATTTTGGCATCCCTCAGCACCTTCCAACAGATGTGGATTGCAAAGGCTGAGTATGATGAGTCTGGCCCCTCAATTGTTCACAGGAAATGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

377

Amino Acids

41.7

Weight (kDa)

5.31

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 5 - 377 2.9e-146 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014601)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03210
fragaria_vesca FvH4_1g27360
malus_domestica MD09G1279600.v1.1
prunus_persica Prupe.3G014700_v2.0.a1
pyrus_communis pycom09g18770
rosa_chinensis RchiOBHm_Chr3g0488741
rosa_laevigata RLG00000022925
rosa_multiflora Rmu_sc0000265.1_g000016 Rmu_sc0001208.1_g000071 Rmu_sc0001208.1_g000072
rosa_roxburghii Rroxscaffold_6G00393910
rosa_rugosa Rorug03G0241200
rosa_samantha Rh3AG289500 Rh3BG326500 Rh3CG324000 Rh3DG322400
rosa_wichuraiana Rw3G025630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 539, 733
Acc65I GGTACC 1 cut(s) 44
AccB1I GGYRCC 2 cut(s) 44, 114
AccBSI CCGCTC 1 cut(s) 622
AccIII TCCGGA 1 cut(s) 905
AciI CCGC 2 cut(s) 622, 1001
AclWI GGATC 1 cut(s) 888
AcuI CTGAAG 1 cut(s) 801
AcyI GRCGYC 2 cut(s) 115, 159
AfaI GTAC 3 cut(s) 46, 404, 448
AfiI CCNNNNNNNGG 3 cut(s) 168, 538, 807
AgsI TTSAA 2 cut(s) 208, 388
AjnI CCWGG 3 cut(s) 414, 543, 925
AleI CACNNNNGTG 2 cut(s) 129, 131
AluBI AGCT 5 cut(s) 92, 286, 705, 818, 968
AluI AGCT 5 cut(s) 92, 286, 705, 818, 968
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 3 cut(s) 374, 630, 683
AlwI GGATC 1 cut(s) 888
AlwNI CAGNNNCTG 1 cut(s) 1023
Ama87I CYCGRG 1 cut(s) 1004
Aor13HI TCCGGA 1 cut(s) 905
AoxI GGCC 3 cut(s) 345, 417, 1102
ApeKI GCWGC 1 cut(s) 818
Asp700I GAANNNNTTC 1 cut(s) 1127
Asp718I GGTACC 1 cut(s) 44
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 2 cut(s) 541, 1103
AsuHPI GGTGA 1 cut(s) 598
AvaI CYCGRG 1 cut(s) 1004
AvaII GGWCC 1 cut(s) 541
BaeI ACNNNNGTAYC 2 cut(s) 28, 61
BanI GGYRCC 2 cut(s) 44, 114
BanII GRGCYC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 288
BbvCI CCTCAGC 1 cut(s) 1052
BbvI GCAGC 1 cut(s) 805
BccI CCATC 7 cut(s) 130, 139, 470, 536, 731, 805, 805
BceAI ACGGC 1 cut(s) 404
BciT130I CCWGG 3 cut(s) 416, 545, 927
BciVI GTATCC 1 cut(s) 836
BcoDI GTCTC 3 cut(s) 374, 630, 683
BfaI CTAG 3 cut(s) 438, 668, 686
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 2 cut(s) 539, 733
BfuI GTATCC 1 cut(s) 836
BglII AGATCT 1 cut(s) 258
BisI GCNGC 1 cut(s) 819
BlpI GCTNAGC 1 cut(s) 618
BlsI GCNGC 1 cut(s) 820
Bme1390I CCNGG 3 cut(s) 416, 545, 927
Bme18I GGWCC 1 cut(s) 541
BmeT110I CYCGRG 1 cut(s) 1004
BmgT120I GGNCC 2 cut(s) 541, 1103
BmiI GGNNCC 6 cut(s) 46, 116, 297, 913, 1036, 1105
BmrFI CCNGG 3 cut(s) 416, 545, 927
BmsI GCATC 6 cut(s) 70, 274, 316, 556, 764, 1055
BpmI CTGGAG 2 cut(s) 93, 282
Bpu10I CCTNAGC 1 cut(s) 1052
Bpu1102I GCTNAGC 1 cut(s) 618
BsaHI GRCGYC 2 cut(s) 115, 159
BsaJI CCNNGG 3 cut(s) 12, 342, 925
BsaWI WCCGGW 2 cut(s) 47, 905
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 538, 807
Bse1I ACTGG 2 cut(s) 311, 457
BseAI TCCGGA 1 cut(s) 905
BseBI CCWGG 3 cut(s) 416, 545, 927
BseDI CCNNGG 3 cut(s) 12, 342, 925
BseLI CCNNNNNNNGG 3 cut(s) 168, 538, 807
BseMII CTCAG 3 cut(s) 609, 1066, 1077
BseNI ACTGG 2 cut(s) 311, 457
BseRI GAGGAG 1 cut(s) 308
BseXI GCAGC 1 cut(s) 805
BseYI CCCAGC 1 cut(s) 101
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 3 cut(s) 347, 419, 1104
BshNI GGYRCC 2 cut(s) 44, 114
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 288
BsiHKCI CYCGRG 1 cut(s) 1004
BsiSI CCGG 2 cut(s) 48, 906
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 3 cut(s) 168, 538, 807
BsmAI GTCTC 3 cut(s) 374, 630, 683
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 3 cut(s) 347, 419, 1104
BsoBI CYCGRG 1 cut(s) 1004
Bsp1286I GDGCHC 1 cut(s) 288
Bsp13I TCCGGA 1 cut(s) 905
Bsp143I GATC 5 cut(s) 216, 258, 556, 804, 880
Bsp1720I GCTNAGC 1 cut(s) 618
BspACI CCGC 2 cut(s) 622, 1001
BspANI GGCC 3 cut(s) 347, 419, 1104
BspCNI CTCAG 3 cut(s) 610, 1065, 1078
BspEI TCCGGA 1 cut(s) 905
BspHI TCATGA 2 cut(s) 573, 852
BspLI GGNNCC 6 cut(s) 46, 116, 297, 913, 1036, 1105
BspMI ACCTGC 2 cut(s) 539, 733
BspPI GGATC 1 cut(s) 888
BspQI GCTCTTC 1 cut(s) 297
BspT107I GGYRCC 2 cut(s) 44, 114
BsrBI CCGCTC 1 cut(s) 622
BsrI ACTGG 2 cut(s) 311, 457
BssECI CCNNGG 3 cut(s) 12, 342, 925
BssMI GATC 5 cut(s) 216, 258, 556, 804, 880
BssNI GRCGYC 2 cut(s) 115, 159
BssT1I CCWWGG 1 cut(s) 342
Bst2UI CCWGG 3 cut(s) 416, 545, 927
Bst4CI ACNGT 3 cut(s) 353, 493, 1019
Bst6I CTCTTC 4 cut(s) 182, 297, 713, 794
BstACI GRCGYC 2 cut(s) 115, 159
BstAPI GCANNNNNTGC 1 cut(s) 68
BstDEI CTNAG 3 cut(s) 618, 1052, 1086
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 5 cut(s) 219, 261, 559, 807, 883
BstMAI GTCTC 3 cut(s) 374, 630, 683
BstMBI GATC 5 cut(s) 216, 258, 556, 804, 880
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
BstNI CCWGG 3 cut(s) 416, 545, 927
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 3 cut(s) 414, 543, 925
BstV1I GCAGC 1 cut(s) 805
BstX2I RGATCY 2 cut(s) 258, 880
BstYI RGATCY 2 cut(s) 258, 880
BsuI GTATCC 1 cut(s) 836
BsuRI GGCC 3 cut(s) 347, 419, 1104
BtsIMutI CAGTG 1 cut(s) 1024
BveI ACCTGC 2 cut(s) 539, 733
CaiI CAGNNNCTG 1 cut(s) 1023
CciI TCATGA 2 cut(s) 573, 852
CfoI GCGC 1 cut(s) 117
Cfr13I GGNCC 2 cut(s) 541, 1103
CseI GACGC 1 cut(s) 167
Csp6I GTAC 3 cut(s) 45, 403, 447
CspCI CAANNNNNGTGG 2 cut(s) 1052, 1087
CviAII CATG 8 cut(s) 224, 524, 574, 640, 802, 830, 853, 979
CviQI GTAC 3 cut(s) 45, 403, 447
DdeI CTNAG 3 cut(s) 618, 1052, 1086
DinI GGCGCC 1 cut(s) 116
DpnI GATC 5 cut(s) 218, 260, 558, 806, 882
DpnII GATC 5 cut(s) 216, 258, 556, 804, 880
Eam1104I CTCTTC 4 cut(s) 182, 297, 713, 794
EarI CTCTTC 4 cut(s) 182, 297, 713, 794
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 342
Eco24I GRGCYC 1 cut(s) 288
Eco32I GATATC 1 cut(s) 871
Eco47I GGWCC 1 cut(s) 541
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 801
Eco88I CYCGRG 1 cut(s) 1004
EcoICRI GAGCTC 1 cut(s) 286
EcoRII CCWGG 3 cut(s) 414, 543, 925
EcoRV GATATC 1 cut(s) 871
EcoT14I CCWWGG 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 288
EgeI GGCGCC 1 cut(s) 116
EheI GGCGCC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 8 cut(s) 227, 527, 577, 643, 805, 833, 856, 982
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FaqI GGGAC 1 cut(s) 162
FatI CATG 8 cut(s) 223, 523, 573, 639, 801, 829, 852, 978
FauI CCCGC 1 cut(s) 615
Fnu4HI GCNGC 1 cut(s) 819
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 1 cut(s) 819
FspBI CTAG 3 cut(s) 438, 668, 686
GlaI GCGC 1 cut(s) 116
GluI GCNGC 1 cut(s) 819
GsaI CCCAGC 1 cut(s) 105
GsuI CTGGAG 2 cut(s) 93, 282
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 3 cut(s) 347, 419, 1104
HapII CCGG 2 cut(s) 48, 906
HgaI GACGC 1 cut(s) 167
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 2 cut(s) 115, 159
Hin1II CATG 8 cut(s) 227, 527, 577, 643, 805, 833, 856, 982
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 6 cut(s) 8, 364, 467, 484, 768, 1097
HpaII CCGG 2 cut(s) 48, 906
HphI GGTGA 1 cut(s) 598
Hpy166II GTNNAC 2 cut(s) 443, 1117
Hpy188I TCNGA 4 cut(s) 13, 201, 498, 773
Hpy8I GTNNAC 2 cut(s) 443, 1117
HpyAV CCTTC 6 cut(s) 148, 500, 542, 637, 871, 1069
HpyCH4III ACNGT 3 cut(s) 353, 493, 1019
HpyCH4IV ACGT 3 cut(s) 405, 445, 592
HpyCH4V TGCA 2 cut(s) 410, 1079
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
HpyF3I CTNAG 3 cut(s) 618, 1052, 1086
HpySE526I ACGT 3 cut(s) 405, 445, 592
Hsp92I GRCGYC 2 cut(s) 115, 159
Hsp92II CATG 8 cut(s) 227, 527, 577, 643, 805, 833, 856, 982
HspAI GCGC 1 cut(s) 115
KasI GGCGCC 1 cut(s) 114
Kpn2I TCCGGA 1 cut(s) 905
KpnI GGTACC 1 cut(s) 48
Kzo9I GATC 5 cut(s) 216, 258, 556, 804, 880
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 6 cut(s) 88, 291, 301, 917, 973, 1040
Lsp1109I GCAGC 1 cut(s) 805
LweI GCATC 6 cut(s) 70, 274, 316, 556, 764, 1055
MaeI CTAG 3 cut(s) 438, 668, 686
MaeII ACGT 3 cut(s) 405, 445, 592
MaeIII GTNAC 3 cut(s) 35, 485, 596
MalI GATC 5 cut(s) 218, 260, 558, 806, 882
MbiI CCGCTC 1 cut(s) 622
MboI GATC 5 cut(s) 216, 258, 556, 804, 880
MboII GAAGA 5 cut(s) 199, 268, 314, 730, 781
MfeI CAATTG 2 cut(s) 238, 1110
MflI RGATCY 2 cut(s) 258, 880
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 7 cut(s) 238, 369, 627, 650, 728, 930, 1110
Mly113I GGCGCC 1 cut(s) 115
MlyI GAGTC 4 cut(s) 358, 461, 493, 1106
MmeI TCCRAC 1 cut(s) 1087
MroI TCCGGA 1 cut(s) 905
MroXI GAANNNNTTC 1 cut(s) 1127
MseI TTAA 1 cut(s) 336
MslI CAYNNNNRTG 2 cut(s) 129, 131
MspI CCGG 2 cut(s) 48, 906
MspR9I CCNGG 3 cut(s) 416, 545, 927
MunI CAATTG 2 cut(s) 238, 1110
MvaI CCWGG 3 cut(s) 416, 545, 927
MwoI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
NarI GGCGCC 1 cut(s) 115
NdeII GATC 5 cut(s) 216, 258, 556, 804, 880
NlaIII CATG 8 cut(s) 227, 527, 577, 643, 805, 833, 856, 982
NlaIV GGNNCC 6 cut(s) 46, 116, 297, 913, 1036, 1105
NmuCI GTSAC 2 cut(s) 35, 485
NspI RCATGY 1 cut(s) 527
OliI CACNNNNGTG 2 cut(s) 129, 131
PagI TCATGA 2 cut(s) 573, 852
PciSI GCTCTTC 1 cut(s) 297
PdmI GAANNNNTTC 1 cut(s) 1127
PfeI GAWTC 2 cut(s) 8, 768
PkrI GCNGC 1 cut(s) 820
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 4 cut(s) 358, 461, 492, 1105
PluTI GGCGCC 1 cut(s) 118
PpsI GAGTC 4 cut(s) 358, 461, 492, 1105
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 3 cut(s) 414, 543, 925
PspFI CCCAGC 1 cut(s) 101
PspGI CCWGG 3 cut(s) 414, 543, 925
PspN4I GGNNCC 6 cut(s) 46, 116, 297, 913, 1036, 1105
PspPI GGNCC 2 cut(s) 541, 1103
PstNI CAGNNNCTG 1 cut(s) 1023
PsuI RGATCY 2 cut(s) 258, 880
PvuI CGATCG 1 cut(s) 219
RsaI GTAC 3 cut(s) 46, 404, 448
RsaNI GTAC 3 cut(s) 45, 403, 447
RseI CAYNNNNRTG 2 cut(s) 129, 131
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 1 cut(s) 819
Sau3AI GATC 5 cut(s) 216, 258, 556, 804, 880
Sau96I GGNCC 2 cut(s) 541, 1103
SchI GAGTC 4 cut(s) 358, 461, 493, 1106
ScrFI CCNGG 3 cut(s) 416, 545, 927
SduI GDGCHC 1 cut(s) 288
SfaNI GCATC 6 cut(s) 70, 274, 316, 556, 764, 1055
SfoI GGCGCC 1 cut(s) 116
SinI GGWCC 1 cut(s) 541
SmiMI CAYNNNNRTG 2 cut(s) 129, 131
Sse9I AATT 7 cut(s) 238, 369, 627, 650, 728, 930, 1110
SsiI CCGC 2 cut(s) 622, 1001
SspDI GGCGCC 1 cut(s) 114
SspMI CTAG 3 cut(s) 438, 668, 686
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 3 cut(s) 414, 543, 925
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 3 cut(s) 353, 493, 1019
TaiI ACGT 3 cut(s) 408, 448, 595
TaqI TCGA 2 cut(s) 185, 219
TasI AATT 7 cut(s) 238, 369, 627, 650, 728, 930, 1110
TfiI GAWTC 2 cut(s) 8, 768
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 1 cut(s) 1024
TseFI GTSAC 2 cut(s) 35, 485
TseI GCWGC 1 cut(s) 818
Tsp45I GTSAC 2 cut(s) 35, 485
TspDTI ATGAA 8 cut(s) 519, 590, 656, 690, 741, 846, 869, 995
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 1 cut(s) 1024
VpaK11BI GGWCC 1 cut(s) 541
XbaI TCTAGA 1 cut(s) 667
XceI RCATGY 1 cut(s) 527
XcmI CCANNNNNNNNNTGG 2 cut(s) 535, 808
XmnI GAANNNNTTC 1 cut(s) 1127
XspI CTAG 3 cut(s) 438, 668, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.