MD09G1280700.v1.1

Notchless protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0017608
Physical Location & Seq
Forward (+)
35789337 .. 35792264
2928 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1280700.v1.1.491

Sequence Viewer

Length: 354 bp
ATGAAAGGCAATGCCCCTGAAAGATTGGTTTCTGGATCTGATGATTTTACTATGTTTCTATGGGAACCTTCTCTTGTAAACCACATCTGCTTTTCACCTGATGGGCAATGGATAGCAAGTGCCTCATTTGATAGGTCTGTCAAGTTATGGAACGGTACTACAGGAAAATTTGTTGCAGCATTCCGAGGCCATGTTGGCCCTGTCTACCAGATCAGCTGGTCGGCAGACAGTAGGCTACTTTTAAGTGGCAGCAAAGATTCAACTCTCAAGGTTTGGGATATCAGGGCACGGGCGCTAAAACAAGACCTTCCAGGCCATGCAGATGAGCATGCTTGGAGGAGGATGGAAACATAG

Protein Analysis

118

Amino Acids

13.15

Weight (kDa)

7.95

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_Aladin PF25460 25 - 95 1.4e-07 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 25 - 108 2.3e-18 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 25 - 112 1.7e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 26 - 107 3.8e-22 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 26 - 106 1.6e-18 THOC3 beta-propeller domain
WD40_Prp19 PF24814 26 - 105 2e-14 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 26 - 62 4e-06 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 26 - 101 1.4e-13 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 26 - 107 3.2e-08 TEP-1 second beta-propeller
WD40 PF00400 26 - 51 4e-09 WD domain, G-beta repeat
WDR55 PF24796 26 - 102 1.4e-10 WDR55
EIF3I PF24805 27 - 95 7.9e-11 EIF3I
WD40_MABP1-WDR62_2nd PF24782 28 - 93 1.1e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd PF25172 28 - 108 7.3e-11 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 33 - 108 1.1e-11 G protein beta WD-40 repeat protein
Beta-prop_TEP1_C PF25048 36 - 73 5.3e-06 TEP-1 C-terminal beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 54 - 97 1.9e-07 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 55 - 93 7.2e-11 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 57 - 106 6.8e-07 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 59 - 97 2.5e-08 CAF1B/HIR1 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 204
AclWI GGATC 1 cut(s) 43
AcsI RAATTY 1 cut(s) 167
AfaI GTAC 1 cut(s) 157
AgsI TTSAA 1 cut(s) 261
AjnI CCWGG 1 cut(s) 310
AluBI AGCT 1 cut(s) 216
AluI AGCT 1 cut(s) 216
AlwI GGATC 1 cut(s) 43
AoxI GGCC 3 cut(s) 187, 196, 313
ApeKI GCWGC 2 cut(s) 176, 249
ApoI RAATTY 1 cut(s) 167
AspLEI GCGC 1 cut(s) 295
AspS9I GGNCC 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 87
BaeGI GKGCMC 1 cut(s) 289
BbvI GCAGC 2 cut(s) 188, 261
BccI CCATC 2 cut(s) 95, 337
BciT130I CCWGG 1 cut(s) 312
BfmI CTRYAG 1 cut(s) 159
BfoI RGCGCY 1 cut(s) 296
BglI GCCNNNNNGGC 1 cut(s) 195
BisI GCNGC 2 cut(s) 177, 250
BlsI GCNGC 2 cut(s) 178, 251
Bme1390I CCNGG 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 1 cut(s) 66
BmrFI CCNGG 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 184
Bse3DI GCAATG 2 cut(s) 16, 113
BseBI CCWGG 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 1 cut(s) 348
BseMI GCAATG 2 cut(s) 16, 113
BseRI GAGGAG 1 cut(s) 352
BseSI GKGCMC 1 cut(s) 289
BseXI GCAGC 2 cut(s) 188, 261
BshFI GGCC 3 cut(s) 189, 198, 315
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 3 cut(s) 189, 198, 315
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 2 cut(s) 35, 210
BspANI GGCC 3 cut(s) 189, 198, 315
BspLI GGNNCC 1 cut(s) 66
BspPI GGATC 1 cut(s) 43
BsrDI GCAATG 2 cut(s) 16, 113
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 2 cut(s) 35, 210
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 2 cut(s) 155, 230
BstC8I GCNNGC 1 cut(s) 330
BstF5I GGATG 1 cut(s) 348
BstH2I RGCGCY 1 cut(s) 296
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 2 cut(s) 38, 213
BstMBI GATC 2 cut(s) 35, 210
BstMWI GCNNNNNNNGC 1 cut(s) 195
BstNI CCWGG 1 cut(s) 312
BstNSI RCATGY 1 cut(s) 332
BstSCI CCNGG 1 cut(s) 310
BstSFI CTRYAG 1 cut(s) 159
BstSLI GKGCMC 1 cut(s) 289
BstV1I GCAGC 2 cut(s) 188, 261
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BsuRI GGCC 3 cut(s) 189, 198, 315
BtsCI GGATG 1 cut(s) 348
Cac8I GCNNGC 1 cut(s) 330
CfoI GCGC 1 cut(s) 295
Cfr13I GGNCC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 156
CviAII CATG 3 cut(s) 191, 317, 329
CviJI RGCY 5 cut(s) 189, 198, 216, 235, 315
CviKI_1 RGCY 5 cut(s) 189, 198, 216, 235, 315
CviQI GTAC 1 cut(s) 156
DpnI GATC 2 cut(s) 37, 212
DpnII GATC 2 cut(s) 35, 210
Eco32I GATATC 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 310
EcoRV GATATC 1 cut(s) 280
FaeI CATG 3 cut(s) 194, 320, 332
FaiI YATR 7 cut(s) 53, 61, 148, 192, 318, 330, 352
FatI CATG 3 cut(s) 190, 316, 328
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 177, 250
Fsp4HI GCNGC 2 cut(s) 177, 250
GlaI GCGC 1 cut(s) 294
GluI GCNGC 2 cut(s) 177, 250
HaeII RGCGCY 1 cut(s) 296
HaeIII GGCC 3 cut(s) 189, 198, 315
HhaI GCGC 1 cut(s) 295
Hin1II CATG 3 cut(s) 194, 320, 332
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HinfI GANTC 1 cut(s) 257
HphI GGTGA 1 cut(s) 87
Hpy166II GTNNAC 2 cut(s) 79, 205
Hpy188I TCNGA 2 cut(s) 40, 185
Hpy188III TCNNGA 1 cut(s) 33
Hpy8I GTNNAC 2 cut(s) 79, 205
HpyAV CCTTC 2 cut(s) 78, 317
HpyCH4III ACNGT 2 cut(s) 155, 230
HpyCH4V TGCA 2 cut(s) 176, 320
HpyF10VI GCNNNNNNNGC 1 cut(s) 195
Hsp92II CATG 3 cut(s) 194, 320, 332
HspAI GCGC 1 cut(s) 293
Kzo9I GATC 2 cut(s) 35, 210
Lsp1109I GCAGC 2 cut(s) 188, 261
MalI GATC 2 cut(s) 37, 212
MboI GATC 2 cut(s) 35, 210
MflI RGATCY 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 1 cut(s) 167
MnlI CCTC 4 cut(s) 133, 179, 330, 333
MseI TTAA 1 cut(s) 242
MslI CAYNNNNRTG 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 216
MspR9I CCNGG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 1 cut(s) 312
MwoI GCNNNNNNNGC 1 cut(s) 195
NdeII GATC 2 cut(s) 35, 210
NlaIII CATG 3 cut(s) 194, 320, 332
NlaIV GGNNCC 1 cut(s) 66
NspI RCATGY 1 cut(s) 332
PaeI GCATGC 1 cut(s) 332
PctI GAATGC 1 cut(s) 179
PfeI GAWTC 1 cut(s) 257
PkrI GCNGC 2 cut(s) 178, 251
Psp6I CCWGG 1 cut(s) 310
PspGI CCWGG 1 cut(s) 310
PspN4I GGNNCC 1 cut(s) 66
PspPI GGNCC 1 cut(s) 197
PsuI RGATCY 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 216
RsaI GTAC 1 cut(s) 157
RsaNI GTAC 1 cut(s) 156
RseI CAYNNNNRTG 1 cut(s) 321
SaqAI TTAA 1 cut(s) 242
SatI GCNGC 2 cut(s) 177, 250
Sau3AI GATC 2 cut(s) 35, 210
Sau96I GGNCC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 1 cut(s) 289
SetI ASST 6 cut(s) 70, 100, 137, 218, 273, 309
SfcI CTRYAG 1 cut(s) 159
SfiI GGCCNNNNNGGCC 1 cut(s) 195
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
SphI GCATGC 1 cut(s) 332
Sse9I AATT 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 310
TaaI ACNGT 2 cut(s) 155, 230
TasI AATT 1 cut(s) 167
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 1 cut(s) 242
Tru9I TTAA 1 cut(s) 242
TseI GCWGC 2 cut(s) 176, 249
TspDTI ATGAA 1 cut(s) 17
XapI RAATTY 1 cut(s) 167
XceI RCATGY 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.