RchiOBHm_Chr3g0486521

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0017608
Physical Location & Seq
Reverse (-)
33406203 .. 33406963
761 bp
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UTR
Exon/CDS
Intron
PRQ45102

Sequence Viewer

Length: 345 bp
ATGTTTGACAACCAAGTAGAACACCGCATTGATCAAAGCAATTCACTCTCAAATGGTGTTCAGCTTCATAATTGCCAGTATGAAGCTATTCATATTAGCAAACTTGCTGGACATGAAGGTTCAATTTTTCGCATATCGTGGTCCTCTAATGGATCAAAACTGGTTTCCATATCTGACGATCGTAGTGCTCGTGTCTGGGCAGTTTTTACTGAAACAATGCATTCTAAGAAGCCAGCGGACTCCTTTGGGCTTATGTTATTTGGTCACAGTGCCCGAGTTTGGGACTGCTGCATTTTTGGATCAGTGAGCTTTACAAAGTGTTTGAACATTGCCTTTTTGTACTAA

Protein Analysis

114

Amino Acids

12.82

Weight (kDa)

7.0

Isoelectric Point (pI)

31.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 13 - 74 1.8e-08 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 20 - 97 1.9e-06 WDR3 first beta-propeller domain
WD40 PF00400 32 - 66 3.1e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 38 - 94 1.5e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 25, 236
AclWI GGATC 2 cut(s) 160, 307
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 2 cut(s) 279, 280
AgsI TTSAA 2 cut(s) 123, 325
AluBI AGCT 3 cut(s) 64, 86, 309
AluI AGCT 3 cut(s) 64, 86, 309
Alw21I GWGCWC 1 cut(s) 190
AlwI GGATC 2 cut(s) 160, 307
Ama87I CYCGRG 1 cut(s) 273
ApeKI GCWGC 1 cut(s) 288
Asp700I GAANNNNTTC 1 cut(s) 87
AspS9I GGNCC 1 cut(s) 141
AvaI CYCGRG 1 cut(s) 273
AvaII GGWCC 1 cut(s) 141
BaeGI GKGCMC 1 cut(s) 274
BauI CACGAG 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 190
BbvI GCAGC 1 cut(s) 275
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BclI TGATCA 1 cut(s) 31
BisI GCNGC 1 cut(s) 289
BlsI GCNGC 1 cut(s) 290
Bme18I GGWCC 1 cut(s) 141
BmeT110I CYCGRG 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 141
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 280
Bse1I ACTGG 2 cut(s) 76, 165
Bse3DI GCAATG 1 cut(s) 327
BseLI CCNNNNNNNGG 2 cut(s) 279, 280
BseMI GCAATG 1 cut(s) 327
BseNI ACTGG 2 cut(s) 76, 165
BseSI GKGCMC 1 cut(s) 274
BseXI GCAGC 1 cut(s) 275
Bsh1285I CGRYCG 1 cut(s) 181
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 190
BsiHKCI CYCGRG 1 cut(s) 273
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 279, 280
BsmFI GGGAC 1 cut(s) 296
BsmI GAATGC 1 cut(s) 220
BsoBI CYCGRG 1 cut(s) 273
Bsp1286I GDGCHC 2 cut(s) 190, 274
Bsp143I GATC 4 cut(s) 31, 152, 178, 299
BspACI CCGC 2 cut(s) 25, 236
BspPI GGATC 2 cut(s) 160, 307
BsrDI GCAATG 1 cut(s) 327
BsrI ACTGG 2 cut(s) 76, 165
BssMI GATC 4 cut(s) 31, 152, 178, 299
BssSI CACGAG 1 cut(s) 189
Bst2BI CACGAG 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 269
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 1 cut(s) 225
BstKTI GATC 4 cut(s) 34, 155, 181, 302
BstMBI GATC 4 cut(s) 31, 152, 178, 299
BstMCI CGRYCG 1 cut(s) 181
BstSLI GKGCMC 1 cut(s) 274
BstV1I GCAGC 1 cut(s) 275
BtsIMutI CAGTG 2 cut(s) 274, 309
Cac8I GCNNGC 1 cut(s) 234
Cfr13I GGNCC 1 cut(s) 141
Csp6I GTAC 1 cut(s) 340
CviAII CATG 1 cut(s) 113
CviJI RGCY 5 cut(s) 64, 86, 232, 250, 309
CviKI_1 RGCY 5 cut(s) 64, 86, 232, 250, 309
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 1 cut(s) 225
DpnI GATC 4 cut(s) 33, 154, 180, 301
DpnII GATC 4 cut(s) 31, 152, 178, 299
Eco47I GGWCC 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 273
EcoT22I ATGCAT 1 cut(s) 222
FaeI CATG 1 cut(s) 116
FaiI YATR 7 cut(s) 69, 81, 93, 114, 134, 170, 254
FaqI GGGAC 1 cut(s) 296
FatI CATG 1 cut(s) 112
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 1 cut(s) 289
Fsp4HI GCNGC 1 cut(s) 289
GluI GCNGC 1 cut(s) 289
Hin1II CATG 1 cut(s) 116
HinfI GANTC 1 cut(s) 239
Hpy188I TCNGA 1 cut(s) 175
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4V TGCA 2 cut(s) 220, 291
HpyF3I CTNAG 1 cut(s) 225
Hsp92II CATG 1 cut(s) 116
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 4 cut(s) 31, 152, 178, 299
LpnPI CCDG 5 cut(s) 89, 93, 146, 181, 246
Lsp1109I GCAGC 1 cut(s) 275
MaeIII GTNAC 1 cut(s) 263
MalI GATC 4 cut(s) 33, 154, 180, 301
MboI GATC 4 cut(s) 31, 152, 178, 299
MhlI GDGCHC 2 cut(s) 190, 274
MluCI AATT 3 cut(s) 40, 70, 123
MlyI GAGTC 1 cut(s) 233
MnlI CCTC 1 cut(s) 154
Mph1103I ATGCAT 1 cut(s) 222
MroXI GAANNNNTTC 1 cut(s) 87
MspA1I CMGCKG 1 cut(s) 236
Mva1269I GAATGC 1 cut(s) 220
NdeII GATC 4 cut(s) 31, 152, 178, 299
NlaIII CATG 1 cut(s) 116
NmuCI GTSAC 1 cut(s) 263
NsiI ATGCAT 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 187
PctI GAATGC 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 87
PkrI GCNGC 1 cut(s) 290
Ple19I CGATCG 1 cut(s) 181
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
PspPI GGNCC 1 cut(s) 141
PvuI CGATCG 1 cut(s) 181
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
SatI GCNGC 1 cut(s) 289
Sau3AI GATC 4 cut(s) 31, 152, 178, 299
Sau96I GGNCC 1 cut(s) 141
SchI GAGTC 1 cut(s) 233
SduI GDGCHC 2 cut(s) 190, 274
SetI ASST 4 cut(s) 66, 88, 121, 311
SinI GGWCC 1 cut(s) 141
Sse9I AATT 3 cut(s) 40, 70, 123
SsiI CCGC 2 cut(s) 25, 236
TaaI ACNGT 1 cut(s) 269
TasI AATT 3 cut(s) 40, 70, 123
TatI WGTACW 1 cut(s) 339
TscAI CASTG 2 cut(s) 274, 309
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 1 cut(s) 288
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 4 cut(s) 56, 80, 96, 129
TspRI CASTG 2 cut(s) 274, 309
VpaK11BI GGWCC 1 cut(s) 141
XmnI GAANNNNTTC 1 cut(s) 87
Zsp2I ATGCAT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.