Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0017608

Physical Location & Seq

Genomic Coordinates Forward (+)

1471819 .. 1477239

Gene Structure 5421 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG018200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1338 bp

ATGCCCCAACTCGCCGAGGCCTACGCCTGCGTGCCGTCGACGGAGCGCGGCCGGGGGATCCTGATCTCCGGTGACCCCAAATCCAACCGGCTCCTCTACACCAATGGCAGATCCGTGATCATCATGAACCTCAACAACCCTCTCGACGTCGCCGTCTACGCCGAGCACGCCTACCCGGCCACCGTCGCCAGATTCTCCCCCAACGGCGAGTGGATCGCGTCCTCCGACGTCTCCGGCACCGTCAGGATCTGGGGGACCCACAACGATTTCGTCCTCAAGAACGAGTTCAAGGTCCTCTCGGGTCGGATCGACGACCTCCAGTGGTCCGCCGACGGAATGAGGATCGTCGCCTGCGGCGAAGGCAAAGGGAAGTCGCTTGTCCGCGCGTTCATGTGGGATTCTGGGTCTACTGTGGGGGATTTCGATGGGCATTCGAAACGGGTTTTGAGCTGTTGTTTCAAGCCAACCAGGCCCTTTCGCATTGTGACATGCGGGGAGGATTTCCTGGTCAATTTCTATGAAGGGCCTCCTTTCAAATTCAAGCAGTCTCACAGGGATCACTCGAATTTTGTGAACTGTGTAAGGTACTCTCCAGATGGGAGTAAGTTTATCACTGTAAGCTCAGATAAGAAGGGTCTTATTTTTGATGGGAAGACTGCGGAGAAGATCGGAGAGCTCTCATCGGAAGATGGCCACAAAGGCAGCATTTATGCTCTTAGCTGGAGTCCTGATGGCAAACAGATTCTGACTGTGTCCGCTGACAAGTCTGCAAAAGTATGGGATATATGTGAGGATGGTAATGGGAAGGTGAATAAAACATTGACATGTCCTGGATCAGGTGGAGTGGATGACATGCTTGTTGGCGGCCTATGGCAGAATGATCATATGGTTACTGTTTCACTTGGAGGCATGATTAGTATATTCTCAGCAAGTGACCTTGATAAAGCCCCAATATCGCTATTTGGACACATGAAGAATATTACTTCATTGGCTGTTCTAAAGAATGACCCGAAACTTATTTTGTCTACCAGCTATGATGGTCTAATTGTCAAGTGGGTTCAAGGCGTTGGATATAGTGGAAAATTACAAAGGAAGGAGACTTCTCAAATAAAATGTTTTGCAGCTGTTGAAGAAGAAATTGTTTCATCTGGATTTGACAATACGGTAACTAGAATTCTTTGTTCTCTTTGTCTCATGCAATTTGCTGATATCTATCTTTACATTTCGATTTCTCTTGAAGAAGAAATTGTTTCATCTGGATTTGACAATATGGTAACTAGACTTCTTTGTTTTTTTTTTGTCTCATGCAATTTGATGGTATCTACCTTTACATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005884 GO:0005938 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0010638 GO:0015629 GO:0016043 GO:0022411 GO:0030029 GO:0030036 GO:0030042 GO:0030832 GO:0030834 GO:0030836 GO:0030863 GO:0030864 GO:0031461 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0032991 GO:0033043 GO:0042641 GO:0042643 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043243 GO:0043244 GO:0043624 GO:0043933 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044877 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051015 GO:0051128 GO:0051130 GO:0051261 GO:0051493 GO:0051495 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080008 GO:0090066 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0110053 GO:1901879 GO:1901881 GO:1902494 GO:1902903 GO:1902905 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

445

Amino Acids

48.85

Weight (kDa)

6.1

Isoelectric Point (pI)

30.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	29 - 203	9.3e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	37 - 210	6.6e-13	WDR3 first beta-propeller domain
Beta-prop_EML	PF23409	53 - 261	8.4e-15	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5	PF25175	55 - 273	1.2e-29	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	70 - 353	2.1e-11	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	102 - 265	5.3e-10	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	102 - 174	4.1e-06	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	104 - 262	1.6e-09	WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	106 - 352	5.8e-09	MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	114 - 263	2.5e-07	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	129 - 264	6.6e-15	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	148 - 262	3.9e-08	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	148 - 392	1.3e-13	WDR55
Beta-prop_WDR3_1st	PF25173	184 - 263	9.5e-10	WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	185 - 264	3.9e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5	PF25175	185 - 361	7.4e-16	WDR5 beta-propeller domain
EIF3I	PF24805	187 - 264	1.3e-08	EIF3I
Beta-prop_THOC3	PF25174	187 - 264	8.3e-13	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	219 - 378	7.2e-09	TEP-1 second beta-propeller
WD40	PF00400	230 - 261	1.1e-08	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017608)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g30420 FvH4_5g16561
malus_domestica	MD09G1280700.v1.1
rosa_chinensis	RchiOBHm_Chr3g0486521 RchiOBHm_Chr5g0062751 RchiOBHm_Chr6g0266701 RchiOBHm_Chr6g0295791
rosa_multiflora	Rmu_sc0014500.1_g000006 Rmu_ssc0000472.1_g000012
rosa_samantha	Rh5AG412300 Rh7DG018200
rosa_wichuraiana	Rw5G043730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	152
AatII	GACGTC	2 cut(s)	150, 231
AccB1I	GGYRCC	1 cut(s)	236
AccI	GTMKAC	4 cut(s)	38, 156, 407, 1025
AccII	CGCG	4 cut(s)	48, 218, 384, 386
AciI	CCGC	8 cut(s)	48, 327, 354, 382, 492, 659, 756, 864
AclWI	GGATC	9 cut(s)	52, 65, 105, 221, 254, 314, 350, 564, 841
AcoI	YGGCCR	3 cut(s)	49, 177, 691
AcsI	RAATTY	3 cut(s)	536, 565, 1173
AcyI	GRCGYC	2 cut(s)	147, 228
AfaI	GTAC	1 cut(s)	587
AfiI	CCNNNNNNNGG	1 cut(s)	836
AflIII	ACRYGT	1 cut(s)	824
AgsI	TTSAA	7 cut(s)	289, 460, 535, 541, 1061, 1130, 1238
AjnI	CCWGG	3 cut(s)	467, 504, 829
AluBI	AGCT	6 cut(s)	450, 621, 676, 720, 1032, 1124
AluI	AGCT	6 cut(s)	450, 621, 676, 720, 1032, 1124
Alw21I	GWGCWC	2 cut(s)	168, 678
Alw26I	GTCTC	5 cut(s)	235, 552, 1091, 1196, 1306
AlwI	GGATC	9 cut(s)	52, 65, 105, 221, 254, 314, 350, 564, 841
AlwNI	CAGNNNCTG	2 cut(s)	249, 745
Ama87I	CYCGRG	1 cut(s)	298
AoxI	GGCC	7 cut(s)	18, 49, 177, 470, 524, 691, 865
ApeKI	GCWGC	2 cut(s)	702, 1121
ApoI	RAATTY	3 cut(s)	536, 565, 1173
Asp700I	GAANNNNTTC	1 cut(s)	284
AspLEI	GCGC	2 cut(s)	48, 386
AspS9I	GGNCC	5 cut(s)	255, 292, 324, 471, 524
AsuC2I	CCSGG	2 cut(s)	53, 176
AsuHPI	GGTGA	2 cut(s)	83, 820
AsuII	TTCGAA	1 cut(s)	434
AvaI	CYCGRG	1 cut(s)	298
AvaII	GGWCC	3 cut(s)	255, 292, 324
BalI	TGGCCA	1 cut(s)	693
BamHI	GGATCC	1 cut(s)	57
BanI	GGYRCC	1 cut(s)	236
BanII	GRGCYC	1 cut(s)	678
BarI	GAAGNNNNNNTAC	2 cut(s)	1266, 1298
BbsI	GAAGAC	1 cut(s)	659
Bbv12I	GWGCWC	2 cut(s)	168, 678
BbvI	GCAGC	2 cut(s)	714, 1133
BccI	CCATC	8 cut(s)	419, 590, 641, 683, 725, 788, 1031, 1309
BceAI	ACGGC	3 cut(s)	19, 137, 220
BciT130I	CCWGG	3 cut(s)	469, 506, 831
BclI	TGATCA	2 cut(s)	117, 880
BcnI	CCSGG	2 cut(s)	53, 176
BcoDI	GTCTC	5 cut(s)	235, 552, 1091, 1196, 1306
BfaI	CTAG	2 cut(s)	1170, 1278
BglI	GCCNNNNNGGC	3 cut(s)	176, 469, 699
BisI	GCNGC	5 cut(s)	49, 355, 703, 865, 1122
BlsI	GCNGC	5 cut(s)	50, 356, 704, 866, 1123
Bme1390I	CCNGG	5 cut(s)	53, 176, 469, 506, 831
Bme18I	GGWCC	3 cut(s)	255, 292, 324
BmeT110I	CYCGRG	1 cut(s)	298
BmgT120I	GGNCC	5 cut(s)	255, 292, 324, 471, 524
BmiI	GGNNCC	5 cut(s)	59, 92, 238, 256, 257
BmrFI	CCNGG	5 cut(s)	53, 176, 469, 506, 831
BpiI	GAAGAC	1 cut(s)	659
BpmI	CTGGAG	3 cut(s)	302, 576, 742
Bpu14I	TTCGAA	1 cut(s)	434
BpuEI	CTTGAG	1 cut(s)	260
BpuMI	CCSGG	2 cut(s)	53, 176
BsaBI	GATNNNNATC	2 cut(s)	62, 1212
BsaHI	GRCGYC	2 cut(s)	147, 228
BsaJI	CCNNGG	2 cut(s)	15, 52
BsaWI	WCCGGW	1 cut(s)	68
Bsc4I	CCNNNNNNNGG	1 cut(s)	836
Bse118I	RCCGGY	1 cut(s)	87
Bse1I	ACTGG	1 cut(s)	319
Bse8I	GATNNNNATC	2 cut(s)	62, 1212
BseBI	CCWGG	3 cut(s)	469, 506, 831
BseDI	CCNNGG	2 cut(s)	15, 52
BseGI	GGATG	2 cut(s)	799, 853
BseJI	GATNNNNATC	2 cut(s)	62, 1212
BseLI	CCNNNNNNNGG	1 cut(s)	836
BseMII	CTCAG	2 cut(s)	636, 939
BseNI	ACTGG	1 cut(s)	319
BseRI	GAGGAG	1 cut(s)	83
BseX3I	CGGCCG	1 cut(s)	49
BseXI	GCAGC	2 cut(s)	714, 1133
Bsh1236I	CGCG	4 cut(s)	48, 218, 384, 386
Bsh1285I	CGRYCG	1 cut(s)	52
BshFI	GGCC	7 cut(s)	20, 51, 179, 472, 526, 693, 867
BshNI	GGYRCC	1 cut(s)	236
BsiEI	CGRYCG	1 cut(s)	52
BsiHKAI	GWGCWC	2 cut(s)	168, 678
BsiHKCI	CYCGRG	1 cut(s)	298
BsiSI	CCGG	5 cut(s)	52, 69, 88, 176, 234
BslFI	GGGAC	1 cut(s)	268
BslI	CCNNNNNNNGG	1 cut(s)	836
BsmAI	GTCTC	5 cut(s)	235, 552, 1091, 1196, 1306
BsmBI	CGTCTC	1 cut(s)	235
BsmFI	GGGAC	1 cut(s)	268
BsmI	GAATGC	1 cut(s)	430
BsnI	GGCC	7 cut(s)	20, 51, 179, 472, 526, 693, 867
BsoBI	CYCGRG	1 cut(s)	298
Bsp119I	TTCGAA	1 cut(s)	434
Bsp1286I	GDGCHC	2 cut(s)	168, 678
BspACI	CCGC	8 cut(s)	48, 327, 354, 382, 492, 659, 756, 864
BspANI	GGCC	7 cut(s)	20, 51, 179, 472, 526, 693, 867
BspCNI	CTCAG	2 cut(s)	635, 938
BspFNI	CGCG	4 cut(s)	48, 218, 384, 386
BspHI	TCATGA	1 cut(s)	123
BspLI	GGNNCC	5 cut(s)	59, 92, 238, 256, 257
BspPI	GGATC	9 cut(s)	52, 65, 105, 221, 254, 314, 350, 564, 841
BspT104I	TTCGAA	1 cut(s)	434
BspT107I	GGYRCC	1 cut(s)	236
BsrFI	RCCGGY	1 cut(s)	87
BsrI	ACTGG	1 cut(s)	319
BssAI	RCCGGY	1 cut(s)	87
BssECI	CCNNGG	2 cut(s)	15, 52
BssNI	GRCGYC	2 cut(s)	147, 228
Bst2UI	CCWGG	3 cut(s)	469, 506, 831
Bst4CI	ACNGT	8 cut(s)	184, 241, 412, 578, 616, 751, 895, 1165
BstACI	GRCGYC	2 cut(s)	147, 228
BstBI	TTCGAA	1 cut(s)	434
BstC8I	GCNNGC	4 cut(s)	28, 32, 168, 352
BstDEI	CTNAG	3 cut(s)	622, 716, 925
BstEII	GGTNACC	1 cut(s)	71
BstENI	CCTNNNNNAGG	1 cut(s)	834
BstF5I	GGATG	2 cut(s)	799, 853
BstFNI	CGCG	4 cut(s)	48, 218, 384, 386
BstHHI	GCGC	2 cut(s)	48, 386
BstMAI	GTCTC	5 cut(s)	235, 552, 1091, 1196, 1306
BstMCI	CGRYCG	1 cut(s)	52
BstMWI	GCNNNNNNNGC	7 cut(s)	158, 167, 176, 185, 360, 469, 699
BstNI	CCWGG	3 cut(s)	469, 506, 831
BstNSI	RCATGY	3 cut(s)	492, 828, 856
BstPI	GGTNACC	1 cut(s)	71
BstSCI	CCNGG	5 cut(s)	51, 174, 467, 504, 829
BstUI	CGCG	4 cut(s)	48, 218, 384, 386
BstV1I	GCAGC	2 cut(s)	714, 1133
BstV2I	GAAGAC	1 cut(s)	659
BstX2I	RGATCY	3 cut(s)	57, 110, 246
BstYI	RGATCY	3 cut(s)	57, 110, 246
BstZI	CGGCCG	1 cut(s)	49
BsuRI	GGCC	7 cut(s)	20, 51, 179, 472, 526, 693, 867
BtsCI	GGATG	2 cut(s)	799, 853
BtsIMutI	CAGTG	2 cut(s)	326, 612
Cac8I	GCNNGC	4 cut(s)	28, 32, 168, 352
CaiI	CAGNNNCTG	2 cut(s)	249, 745
CciI	TCATGA	1 cut(s)	123
CfoI	GCGC	2 cut(s)	48, 386
Cfr10I	RCCGGY	1 cut(s)	87
Cfr13I	GGNCC	5 cut(s)	255, 292, 324, 471, 524
CseI	GACGC	1 cut(s)	207
Csp6I	GTAC	1 cut(s)	586
CviAII	CATG	9 cut(s)	124, 391, 489, 825, 853, 910, 970, 1195, 1305
CviQI	GTAC	1 cut(s)	586
DdeI	CTNAG	3 cut(s)	622, 716, 925
DrdI	GACNNNNNNGTC	1 cut(s)	152
DseDI	GACNNNNNNGTC	1 cut(s)	152
EaeI	YGGCCR	3 cut(s)	49, 177, 691
EagI	CGGCCG	1 cut(s)	49
EciI	GGCGGA	1 cut(s)	316
Ecl136II	GAGCTC	1 cut(s)	676
EclXI	CGGCCG	1 cut(s)	49
Eco147I	AGGCCT	1 cut(s)	20
Eco24I	GRGCYC	1 cut(s)	678
Eco32I	GATATC	1 cut(s)	1210
Eco47I	GGWCC	3 cut(s)	255, 292, 324
Eco52I	CGGCCG	1 cut(s)	49
Eco53kI	GAGCTC	1 cut(s)	676
Eco88I	CYCGRG	1 cut(s)	298
Eco91I	GGTNACC	1 cut(s)	71
EcoICRI	GAGCTC	1 cut(s)	676
EcoNI	CCTNNNNNAGG	1 cut(s)	834
EcoO109I	RGGNCCY	4 cut(s)	255, 292, 471, 524
EcoO65I	GGTNACC	1 cut(s)	71
EcoRI	GAATTC	1 cut(s)	1173
EcoRII	CCWGG	3 cut(s)	467, 504, 829
EcoRV	GATATC	1 cut(s)	1210
EcoT38I	GRGCYC	1 cut(s)	678
Esp3I	CGTCTC	1 cut(s)	235
FaeI	CATG	9 cut(s)	127, 394, 492, 828, 856, 913, 973, 1198, 1308
FaqI	GGGAC	1 cut(s)	268
FatI	CATG	9 cut(s)	123, 390, 488, 824, 852, 909, 969, 1194, 1304
FauI	CCCGC	1 cut(s)	485
FauNDI	CATATG	1 cut(s)	885
FbaI	TGATCA	2 cut(s)	117, 880
FblI	GTMKAC	4 cut(s)	38, 156, 407, 1025
Fnu4HI	GCNGC	5 cut(s)	49, 355, 703, 865, 1122
FokI	GGATG	2 cut(s)	806, 860
FriOI	GRGCYC	1 cut(s)	678
Fsp4HI	GCNGC	5 cut(s)	49, 355, 703, 865, 1122
FspBI	CTAG	2 cut(s)	1170, 1278
GlaI	GCGC	2 cut(s)	47, 385
GluI	GCNGC	5 cut(s)	49, 355, 703, 865, 1122
GsuI	CTGGAG	3 cut(s)	302, 576, 742
HaeIII	GGCC	7 cut(s)	20, 51, 179, 472, 526, 693, 867
HapII	CCGG	5 cut(s)	52, 69, 88, 176, 234
HgaI	GACGC	1 cut(s)	207
HhaI	GCGC	2 cut(s)	48, 386
Hin1I	GRCGYC	2 cut(s)	147, 228
Hin1II	CATG	9 cut(s)	127, 394, 492, 828, 856, 913, 973, 1198, 1308
Hin6I	GCGC	2 cut(s)	46, 384
HinP1I	GCGC	2 cut(s)	46, 384
HincII	GTYRAC	1 cut(s)	39
HindII	GTYRAC	1 cut(s)	39
HinfI	GANTC	4 cut(s)	192, 398, 724, 742
HpaII	CCGG	5 cut(s)	52, 69, 88, 176, 234
HphI	GGTGA	2 cut(s)	83, 820
Hpy166II	GTNNAC	5 cut(s)	39, 157, 408, 574, 1026
Hpy188I	TCNGA	6 cut(s)	226, 306, 625, 671, 685, 747
Hpy8I	GTNNAC	5 cut(s)	39, 157, 408, 574, 1026
Hpy99I	CGWCG	9 cut(s)	40, 43, 149, 152, 188, 230, 314, 335, 350
HpyAV	CCTTC	5 cut(s)	353, 515, 625, 799, 1087
HpyCH4III	ACNGT	8 cut(s)	184, 241, 412, 578, 616, 751, 895, 1165
HpyCH4IV	ACGT	2 cut(s)	147, 228
HpyCH4V	TGCA	4 cut(s)	770, 1121, 1198, 1308
HpyF10VI	GCNNNNNNNGC	7 cut(s)	158, 167, 176, 185, 360, 469, 699
HpyF3I	CTNAG	3 cut(s)	622, 716, 925
HpySE526I	ACGT	2 cut(s)	147, 228
Hsp92I	GRCGYC	2 cut(s)	147, 228
Hsp92II	CATG	9 cut(s)	127, 394, 492, 828, 856, 913, 973, 1198, 1308
HspAI	GCGC	2 cut(s)	46, 384
KflI	GGGWCCC	1 cut(s)	255
Ksp22I	TGATCA	2 cut(s)	117, 880
LmnI	GCTCC	2 cut(s)	43, 96
Lsp1109I	GCAGC	2 cut(s)	714, 1133
MaeI	CTAG	2 cut(s)	1170, 1278
MaeII	ACGT	2 cut(s)	147, 228
MaeIII	GTNAC	6 cut(s)	71, 484, 889, 932, 1165, 1273
MboII	GAAGA	8 cut(s)	664, 676, 698, 985, 1142, 1145, 1250, 1253
MflI	RGATCY	3 cut(s)	57, 110, 246
MhlI	GDGCHC	2 cut(s)	168, 678
MlsI	TGGCCA	1 cut(s)	693
MluNI	TGGCCA	1 cut(s)	693
MlyI	GAGTC	1 cut(s)	733
MmeI	TCCRAC	4 cut(s)	108, 249, 284, 1048
Mox20I	TGGCCA	1 cut(s)	693
MroXI	GAANNNNTTC	1 cut(s)	284
MscI	TGGCCA	1 cut(s)	693
MslI	CAYNNNNRTG	1 cut(s)	823
Msp20I	TGGCCA	1 cut(s)	693
MspA1I	CMGCKG	2 cut(s)	758, 1124
MspI	CCGG	5 cut(s)	52, 69, 88, 176, 234
MspR9I	CCNGG	5 cut(s)	53, 176, 469, 506, 831
Mva1269I	GAATGC	1 cut(s)	430
MvaI	CCWGG	3 cut(s)	469, 506, 831
MvnI	CGCG	4 cut(s)	48, 218, 384, 386
MwoI	GCNNNNNNNGC	7 cut(s)	158, 167, 176, 185, 360, 469, 699
NciI	CCSGG	2 cut(s)	53, 176
NdeI	CATATG	1 cut(s)	885
NlaIII	CATG	9 cut(s)	127, 394, 492, 828, 856, 913, 973, 1198, 1308
NlaIV	GGNNCC	5 cut(s)	59, 92, 238, 256, 257
NmeAIII	GCCGAG	2 cut(s)	40, 187
NmuCI	GTSAC	3 cut(s)	71, 484, 932
NspI	RCATGY	3 cut(s)	492, 828, 856
NspV	TTCGAA	1 cut(s)	434
PagI	TCATGA	1 cut(s)	123
PceI	AGGCCT	1 cut(s)	20
PciI	ACATGT	1 cut(s)	824
PcsI	WCGNNNNNNNCGW	3 cut(s)	150, 222, 354
PctI	GAATGC	1 cut(s)	430
PdmI	GAANNNNTTC	1 cut(s)	284
PfeI	GAWTC	3 cut(s)	192, 398, 742
PflFI	GACNNNGTC	1 cut(s)	751
PfoI	TCCNGGA	1 cut(s)	829
PkrI	GCNGC	5 cut(s)	50, 356, 704, 866, 1123
PleI	GAGTC	1 cut(s)	732
PpsI	GAGTC	1 cut(s)	732
PpuMI	RGGWCCY	2 cut(s)	255, 292
PscI	ACATGT	1 cut(s)	824
Psp124BI	GAGCTC	1 cut(s)	678
Psp5II	RGGWCCY	2 cut(s)	255, 292
Psp6I	CCWGG	3 cut(s)	467, 504, 829
PspEI	GGTNACC	1 cut(s)	71
PspGI	CCWGG	3 cut(s)	467, 504, 829
PspN4I	GGNNCC	5 cut(s)	59, 92, 238, 256, 257
PspPI	GGNCC	5 cut(s)	255, 292, 324, 471, 524
PspPPI	RGGWCCY	2 cut(s)	255, 292
PstNI	CAGNNNCTG	2 cut(s)	249, 745
PsuI	RGATCY	3 cut(s)	57, 110, 246
PsyI	GACNNNGTC	1 cut(s)	751
PvuII	CAGCTG	1 cut(s)	1124
RsaI	GTAC	1 cut(s)	587
RsaNI	GTAC	1 cut(s)	586
RseI	CAYNNNNRTG	1 cut(s)	823
SacI	GAGCTC	1 cut(s)	678
SalI	GTCGAC	1 cut(s)	37
SatI	GCNGC	5 cut(s)	49, 355, 703, 865, 1122
Sau96I	GGNCC	5 cut(s)	255, 292, 324, 471, 524
SchI	GAGTC	1 cut(s)	733
ScrFI	CCNGG	5 cut(s)	53, 176, 469, 506, 831
SduI	GDGCHC	2 cut(s)	168, 678
SfuI	TTCGAA	1 cut(s)	434
SgrDI	CGTCGACG	1 cut(s)	37
SinI	GGWCC	3 cut(s)	255, 292, 324
SmiMI	CAYNNNNRTG	1 cut(s)	823
SmlI	CTYRAG	1 cut(s)	275
SmoI	CTYRAG	1 cut(s)	275
SseBI	AGGCCT	1 cut(s)	20
SsiI	CCGC	8 cut(s)	48, 327, 354, 382, 492, 659, 756, 864
SspI	AATATT	1 cut(s)	979
SspMI	CTAG	2 cut(s)	1170, 1278
SstI	GAGCTC	1 cut(s)	678
StuI	AGGCCT	1 cut(s)	20
StyD4I	CCNGG	5 cut(s)	51, 174, 467, 504, 829
TaaI	ACNGT	8 cut(s)	184, 241, 412, 578, 616, 751, 895, 1165
TaiI	ACGT	2 cut(s)	150, 231
TaqI	TCGA	7 cut(s)	38, 144, 309, 423, 434, 563, 1226
TauI	GCSGC	3 cut(s)	51, 357, 867
TfiI	GAWTC	3 cut(s)	192, 398, 742
TscAI	CASTG	2 cut(s)	326, 619
TseFI	GTSAC	3 cut(s)	71, 484, 932
TseI	GCWGC	2 cut(s)	702, 1121
Tsp45I	GTSAC	3 cut(s)	71, 484, 932
TspDTI	ATGAA	7 cut(s)	140, 379, 534, 975, 986, 1134, 1242
TspGWI	ACGGA	3 cut(s)	56, 103, 348
TspRI	CASTG	2 cut(s)	326, 619
Tth111I	GACNNNGTC	1 cut(s)	751
VpaK11BI	GGWCC	3 cut(s)	255, 292, 324
XagI	CCTNNNNNAGG	1 cut(s)	834
XapI	RAATTY	3 cut(s)	536, 565, 1173
XceI	RCATGY	3 cut(s)	492, 828, 856
XmiI	GTMKAC	4 cut(s)	38, 156, 407, 1025
XmnI	GAANNNNTTC	1 cut(s)	284
XspI	CTAG	2 cut(s)	1170, 1278
ZraI	GACGTC	2 cut(s)	148, 229

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.