MD09G1288800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013474
Physical Location & Seq
Forward (+)
36841154 .. 36844515
3362 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1288800.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGTCTTCGACTCCGGCAATGGCGCTGCATGTTTCCTGGTACGACTTCGTCTGCGTCGCCATTGTTGGGGTTTCTTTTCTGGCGGCGTCGTGGGTGATCTGGAGGAAGGAAGGAGCTTCGGAGAGATTCGAAGACAGCACAATGTACGAGAGCCTTCTGGTGAGCCGCCCTGACAGCCACGTGTTTGTCAGCGCCCTTCCGAGGGGCCACGTCAGCACCTCCCAGCTCTGGACTAGCTGCTGGAAAGGGCTCCACCCCGGCTGGCTCTTCGCCTTTCGATTCGTCTCGTTTCTGGCTTTGGCTGGGTTCTTGGCTTGGGATCTTGTCGAATGGGATGCTTCCATTTTCATTTACTACACCGAGTGGACATTTACACTGGTTATGATCTATTTTGCGCTGGGAACTCTAGTGTCTGGGTATGGTTGTTGGTTGTCCTTAAACAAGCCCCCCACTGAAAATGGGACAAGGTCAGAAGCATTGAGAAGGGATGTGGAAGAGAATAATGGAACTGTGAATACCCATAGGCGAAACGGAATTAAGAGTACCATCAAATTGCAAAGCCAGTATGCTCAGGAGGAGATTCAGAAACGAACAGGATATTGGGGATATCTTATGCAAATTGCATTCCAGACTTGTGCAGGAGCTGTTGTCCTCACAGATGTCGTCTTTTGGTGTATCATCCTCCCATTTTTATCAAATGCTCACCTTGGCCTCAATACGTTGATGTGTTGCATGCATATGCTGAATGCAGCGTTCCTTCTTCTGGATACCATTCTCAACAGCCTTCCATTTCCATGGTTCCGGCTTTCATATTTTGTCCTCTGGAGCTGCATCTACATTGCCTTCCAGTGGGTCATTCATGCATTAGGTTTCCCATGGTGGCCGTATCCATTTCTTGAGCTCAATACGCCATGGGCTCCATTATGGTATTTTTGCGGGGCTGTGGCTCACATCCCTTGCTATGGGATTTTTGCAGTGATTATAAAAGCAAAGTATTCACTTCTGCCCAAATTGTTTCCCCACTCTTTTGTGAGGCCATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

348

Amino Acids

39.87

Weight (kDa)

7.58

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47115
fragaria_vesca FvH4_1g26530 FvH4_1g26530
malus_domestica MD09G1288800.v1.1 MD17G1280700.v1.1
prunus_persica Prupe.3G004800_v2.0.a1
pyrus_communis pycom09g19580 pycom17g27850
rosa_chinensis RchiOBHm_Chr3g0486611
rosa_laevigata RLG00000023073
rosa_multiflora Rmu_sc0001508.1_g000003
rosa_roxburghii Rroxscaffold_6G00395770
rosa_rugosa Rorug03G0225600 Rorug03G0225700
rosa_samantha Rh3AG275500 Rh3CG309900 Rh3DG306600
rosa_wichuraiana Rw3G024550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 983
AciI CCGC 3 cut(s) 83, 166, 936
AclWI GGATC 1 cut(s) 327
AcoI YGGCCR 1 cut(s) 881
AcvI CACGTG 1 cut(s) 181
AcyI GRCGYC 1 cut(s) 86
AdeI CACNNNGTG 1 cut(s) 363
AfaI GTAC 3 cut(s) 41, 146, 544
AfiI CCNNNNNNNGG 7 cut(s) 66, 201, 202, 228, 763, 849, 962
AflIII ACRYGT 1 cut(s) 180
AjiI CACGTC 1 cut(s) 211
AjnI CCWGG 1 cut(s) 35
AjuI GAANNNNNNNTTGG 2 cut(s) 583, 615
AluBI AGCT 6 cut(s) 116, 226, 237, 644, 828, 901
AluI AGCT 6 cut(s) 116, 226, 237, 644, 828, 901
Alw21I GWGCWC 1 cut(s) 903
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 1 cut(s) 327
AlwNI CAGNNNCTG 1 cut(s) 644
AoxI GGCC 4 cut(s) 205, 709, 881, 1034
ApeKI GCWGC 4 cut(s) 25, 237, 749, 828
AspLEI GCGC 3 cut(s) 25, 194, 397
AspS9I GGNCC 1 cut(s) 205
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 3 cut(s) 106, 172, 695
AsuII TTCGAA 1 cut(s) 129
BanII GRGCYC 3 cut(s) 252, 903, 919
BbrPI CACGTG 1 cut(s) 181
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 903
BbvI GCAGC 4 cut(s) 12, 224, 761, 815
BccI CCATC 1 cut(s) 554
BceAI ACGGC 1 cut(s) 868
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 1 cut(s) 37
BciVI GTATCC 2 cut(s) 760, 897
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 3 cut(s) 234, 407, 1042
BfoI RGCGCY 2 cut(s) 26, 195
BfuI GTATCC 2 cut(s) 760, 897
BisI GCNGC 6 cut(s) 26, 84, 166, 238, 750, 829
BlsI GCNGC 6 cut(s) 27, 85, 167, 239, 751, 830
Bme1390I CCNGG 2 cut(s) 37, 258
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 4 cut(s) 206, 251, 800, 918
BmrFI CCNGG 2 cut(s) 37, 258
BmsI GCATC 2 cut(s) 325, 840
BpiI GAAGAC 1 cut(s) 138
BpmI CTGGAG 2 cut(s) 121, 844
Bpu10I CCTNAGC 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 917
BpuMI CCSGG 1 cut(s) 258
BsaAI YACGTR 1 cut(s) 181
BsaHI GRCGYC 1 cut(s) 86
BsaJI CCNNGG 6 cut(s) 200, 256, 706, 794, 875, 911
BsaXI ACNNNNNCTCC 2 cut(s) 633, 663
Bsc4I CCNNNNNNNGG 7 cut(s) 66, 201, 202, 228, 763, 849, 962
Bse1I ACTGG 3 cut(s) 381, 562, 847
Bse3DI GCAATG 2 cut(s) 24, 837
BseBI CCWGG 1 cut(s) 37
BseDI CCNNGG 6 cut(s) 200, 256, 706, 794, 875, 911
BseGI GGATG 4 cut(s) 340, 493, 678, 951
BseLI CCNNNNNNNGG 7 cut(s) 66, 201, 202, 228, 763, 849, 962
BseMI GCAATG 2 cut(s) 24, 837
BseMII CTCAG 1 cut(s) 584
BseNI ACTGG 3 cut(s) 381, 562, 847
BseRI GAGGAG 1 cut(s) 590
BseXI GCAGC 4 cut(s) 12, 224, 761, 815
BseYI CCCAGC 3 cut(s) 222, 302, 397
BsgI GTGCAG 1 cut(s) 657
BshFI GGCC 4 cut(s) 207, 711, 883, 1036
BsiHKAI GWGCWC 1 cut(s) 903
BsiSI CCGG 3 cut(s) 14, 258, 802
BslFI GGGAC 1 cut(s) 475
BslI CCNNNNNNNGG 7 cut(s) 66, 201, 202, 228, 763, 849, 962
BsmAI GTCTC 1 cut(s) 289
BsmBI CGTCTC 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 475
BsmI GAATGC 2 cut(s) 623, 751
BsnI GGCC 4 cut(s) 207, 711, 883, 1036
Bsp119I TTCGAA 1 cut(s) 129
Bsp1286I GDGCHC 3 cut(s) 252, 903, 919
Bsp143I GATC 3 cut(s) 96, 319, 384
Bsp19I CCATGG 3 cut(s) 794, 875, 911
BspACI CCGC 3 cut(s) 83, 166, 936
BspANI GGCC 4 cut(s) 207, 711, 883, 1036
BspCNI CTCAG 1 cut(s) 583
BspLI GGNNCC 4 cut(s) 206, 251, 800, 918
BspPI GGATC 1 cut(s) 327
BspQI GCTCTTC 1 cut(s) 272
BspT104I TTCGAA 1 cut(s) 129
BsrDI GCAATG 2 cut(s) 24, 837
BsrI ACTGG 3 cut(s) 381, 562, 847
BssECI CCNNGG 6 cut(s) 200, 256, 706, 794, 875, 911
BssMI GATC 3 cut(s) 96, 319, 384
BssNI GRCGYC 1 cut(s) 86
BssT1I CCWWGG 4 cut(s) 706, 794, 875, 911
Bst2UI CCWGG 1 cut(s) 37
Bst4CI ACNGT 1 cut(s) 511
Bst6I CTCTTC 2 cut(s) 272, 489
BstACI GRCGYC 1 cut(s) 86
BstBAI YACGTR 1 cut(s) 181
BstBI TTCGAA 1 cut(s) 129
BstC8I GCNNGC 2 cut(s) 263, 734
BstDEI CTNAG 1 cut(s) 570
BstDSI CCRYGG 3 cut(s) 794, 875, 911
BstF5I GGATG 4 cut(s) 340, 493, 678, 951
BstH2I RGCGCY 2 cut(s) 26, 195
BstHHI GCGC 3 cut(s) 25, 194, 397
BstKTI GATC 3 cut(s) 99, 322, 387
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 3 cut(s) 96, 319, 384
BstMWI GCNNNNNNNGC 3 cut(s) 174, 213, 907
BstNI CCWGG 1 cut(s) 37
BstNSI RCATGY 2 cut(s) 32, 736
BstSCI CCNGG 2 cut(s) 35, 256
BstV1I GCAGC 4 cut(s) 12, 224, 761, 815
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 319
BstXI CCANNNNNNTGG 1 cut(s) 795
BstYI RGATCY 1 cut(s) 319
BsuI GTATCC 2 cut(s) 760, 897
BsuRI GGCC 4 cut(s) 207, 711, 883, 1036
BtgI CCRYGG 3 cut(s) 794, 875, 911
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 4 cut(s) 340, 493, 678, 951
BtsI GCAGTG 1 cut(s) 981
BtsIMutI CAGTG 4 cut(s) 374, 450, 854, 981
Cac8I GCNNGC 2 cut(s) 263, 734
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 3 cut(s) 25, 194, 397
Cfr13I GGNCC 1 cut(s) 205
CseI GACGC 2 cut(s) 43, 75
Csp6I GTAC 3 cut(s) 40, 145, 543
CviAII CATG 6 cut(s) 29, 733, 795, 860, 876, 912
CviQI GTAC 3 cut(s) 40, 145, 543
DdeI CTNAG 1 cut(s) 570
DpnI GATC 3 cut(s) 98, 321, 386
DpnII GATC 3 cut(s) 96, 319, 384
DraIII CACNNNGTG 1 cut(s) 363
EaeI YGGCCR 1 cut(s) 881
Eam1104I CTCTTC 2 cut(s) 272, 489
EarI CTCTTC 2 cut(s) 272, 489
Ecl136II GAGCTC 1 cut(s) 901
Eco130I CCWWGG 4 cut(s) 706, 794, 875, 911
Eco24I GRGCYC 3 cut(s) 252, 903, 919
Eco32I GATATC 1 cut(s) 608
Eco53kI GAGCTC 1 cut(s) 901
Eco72I CACGTG 1 cut(s) 181
EcoICRI GAGCTC 1 cut(s) 901
EcoRII CCWGG 1 cut(s) 35
EcoRV GATATC 1 cut(s) 608
EcoT14I CCWWGG 4 cut(s) 706, 794, 875, 911
EcoT22I ATGCAT 2 cut(s) 738, 865
EcoT38I GRGCYC 3 cut(s) 252, 903, 919
ErhI CCWWGG 4 cut(s) 706, 794, 875, 911
Esp3I CGTCTC 1 cut(s) 289
FaeI CATG 6 cut(s) 32, 736, 798, 863, 879, 915
FaqI GGGAC 1 cut(s) 475
FatI CATG 6 cut(s) 28, 732, 794, 859, 875, 911
FauI CCCGC 1 cut(s) 929
FauNDI CATATG 1 cut(s) 738
Fnu4HI GCNGC 6 cut(s) 26, 84, 166, 238, 750, 829
FokI GGATG 4 cut(s) 347, 500, 665, 938
FriOI GRGCYC 3 cut(s) 252, 903, 919
Fsp4HI GCNGC 6 cut(s) 26, 84, 166, 238, 750, 829
FspBI CTAG 3 cut(s) 234, 407, 1042
GlaI GCGC 3 cut(s) 24, 193, 396
GluI GCNGC 6 cut(s) 26, 84, 166, 238, 750, 829
GsaI CCCAGC 3 cut(s) 226, 306, 401
GsuI CTGGAG 2 cut(s) 121, 844
HaeII RGCGCY 2 cut(s) 26, 195
HaeIII GGCC 4 cut(s) 207, 711, 883, 1036
HapII CCGG 3 cut(s) 14, 258, 802
HgaI GACGC 2 cut(s) 43, 75
HhaI GCGC 3 cut(s) 25, 194, 397
Hin1I GRCGYC 1 cut(s) 86
Hin1II CATG 6 cut(s) 32, 736, 798, 863, 879, 915
Hin6I GCGC 3 cut(s) 23, 192, 395
HinP1I GCGC 3 cut(s) 23, 192, 395
HinfI GANTC 4 cut(s) 10, 126, 279, 580
HpaII CCGG 3 cut(s) 14, 258, 802
HphI GGTGA 3 cut(s) 106, 172, 695
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 4 cut(s) 121, 201, 472, 585
Hpy188III TCNNGA 7 cut(s) 100, 229, 572, 628, 764, 823, 896
Hpy8I GTNNAC 1 cut(s) 366
Hpy99I CGWCG 2 cut(s) 59, 91
HpyAV CCTTC 8 cut(s) 100, 104, 164, 206, 477, 767, 794, 853
HpyCH4III ACNGT 1 cut(s) 511
HpyCH4IV ACGT 3 cut(s) 180, 210, 719
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 213, 907
HpyF3I CTNAG 1 cut(s) 570
HpySE526I ACGT 3 cut(s) 180, 210, 719
Hsp92I GRCGYC 1 cut(s) 86
Hsp92II CATG 6 cut(s) 32, 736, 798, 863, 879, 915
HspAI GCGC 3 cut(s) 23, 192, 395
Kzo9I GATC 3 cut(s) 96, 319, 384
LguI GCTCTTC 1 cut(s) 272
LmnI GCTCC 5 cut(s) 113, 255, 641, 825, 922
Lsp1109I GCAGC 4 cut(s) 12, 224, 761, 815
LweI GCATC 2 cut(s) 325, 840
MaeI CTAG 3 cut(s) 234, 407, 1042
MaeII ACGT 3 cut(s) 180, 210, 719
MalI GATC 3 cut(s) 98, 321, 386
MboI GATC 3 cut(s) 96, 319, 384
MboII GAAGA 4 cut(s) 143, 259, 506, 752
MflI RGATCY 1 cut(s) 319
MhlI GDGCHC 3 cut(s) 252, 903, 919
MluCI AATT 4 cut(s) 534, 551, 618, 1010
MlyI GAGTC 1 cut(s) 4
MnlI CCTC 9 cut(s) 96, 195, 229, 568, 662, 692, 722, 830, 1026
Mph1103I ATGCAT 2 cut(s) 738, 865
MseI TTAA 2 cut(s) 437, 537
MslI CAYNNNNRTG 2 cut(s) 737, 793
MspI CCGG 3 cut(s) 14, 258, 802
MspR9I CCNGG 2 cut(s) 37, 258
Mva1269I GAATGC 2 cut(s) 623, 751
MvaI CCWGG 1 cut(s) 37
MwoI GCNNNNNNNGC 3 cut(s) 174, 213, 907
NciI CCSGG 1 cut(s) 258
NcoI CCATGG 3 cut(s) 794, 875, 911
NdeI CATATG 1 cut(s) 738
NdeII GATC 3 cut(s) 96, 319, 384
NlaIII CATG 6 cut(s) 32, 736, 798, 863, 879, 915
NlaIV GGNNCC 4 cut(s) 206, 251, 800, 918
NsiI ATGCAT 2 cut(s) 738, 865
NspI RCATGY 2 cut(s) 32, 736
NspV TTCGAA 1 cut(s) 129
PaeI GCATGC 1 cut(s) 736
PciSI GCTCTTC 1 cut(s) 272
PctI GAATGC 2 cut(s) 623, 751
PfeI GAWTC 3 cut(s) 126, 279, 580
PflFI GACNNNGTC 2 cut(s) 47, 466
PkrI GCNGC 6 cut(s) 27, 85, 167, 239, 751, 830
PleI GAGTC 1 cut(s) 4
PmaCI CACGTG 1 cut(s) 181
PmlI CACGTG 1 cut(s) 181
PpsI GAGTC 1 cut(s) 4
Ppu21I YACGTR 1 cut(s) 181
PsiI TTATAA 1 cut(s) 983
Psp124BI GAGCTC 1 cut(s) 903
Psp6I CCWGG 1 cut(s) 35
PspCI CACGTG 1 cut(s) 181
PspFI CCCAGC 3 cut(s) 222, 302, 397
PspGI CCWGG 1 cut(s) 35
PspN4I GGNNCC 4 cut(s) 206, 251, 800, 918
PspPI GGNCC 1 cut(s) 205
PsrI GAACNNNNNNTAC 2 cut(s) 499, 531
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 1 cut(s) 319
PsyI GACNNNGTC 2 cut(s) 47, 466
RsaI GTAC 3 cut(s) 41, 146, 544
RsaNI GTAC 3 cut(s) 40, 145, 543
RseI CAYNNNNRTG 2 cut(s) 737, 793
SacI GAGCTC 1 cut(s) 903
SapI GCTCTTC 1 cut(s) 272
SaqAI TTAA 2 cut(s) 437, 537
SatI GCNGC 6 cut(s) 26, 84, 166, 238, 750, 829
Sau3AI GATC 3 cut(s) 96, 319, 384
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 1 cut(s) 4
ScrFI CCNGG 2 cut(s) 37, 258
SduI GDGCHC 3 cut(s) 252, 903, 919
SfaNI GCATC 2 cut(s) 325, 840
SfuI TTCGAA 1 cut(s) 129
SmiMI CAYNNNNRTG 2 cut(s) 737, 793
SmlI CTYRAG 1 cut(s) 896
SmoI CTYRAG 1 cut(s) 896
SphI GCATGC 1 cut(s) 736
Sse9I AATT 4 cut(s) 534, 551, 618, 1010
SsiI CCGC 3 cut(s) 83, 166, 936
SspMI CTAG 3 cut(s) 234, 407, 1042
SstI GAGCTC 1 cut(s) 903
StyD4I CCNGG 2 cut(s) 35, 256
StyI CCWWGG 4 cut(s) 706, 794, 875, 911
TaaI ACNGT 1 cut(s) 511
TaiI ACGT 3 cut(s) 183, 213, 722
TaqI TCGA 4 cut(s) 8, 129, 277, 327
TasI AATT 4 cut(s) 534, 551, 618, 1010
TauI GCSGC 2 cut(s) 86, 168
TfiI GAWTC 3 cut(s) 126, 279, 580
Tru1I TTAA 2 cut(s) 437, 537
Tru9I TTAA 2 cut(s) 437, 537
TscAI CASTG 4 cut(s) 381, 457, 854, 981
TseI GCWGC 4 cut(s) 25, 237, 749, 828
TspDTI ATGAA 3 cut(s) 337, 798, 848
TspGWI ACGGA 1 cut(s) 546
TspRI CASTG 4 cut(s) 381, 457, 854, 981
Tth111I GACNNNGTC 2 cut(s) 47, 466
XceI RCATGY 2 cut(s) 32, 736
XspI CTAG 3 cut(s) 234, 407, 1042
Zsp2I ATGCAT 2 cut(s) 738, 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.