Rorug03G0225700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0013474
Physical Location & Seq
Forward (+)
20717216 .. 20720705
3490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0225700.1

Sequence Viewer

Length: 1230 bp
ATGGGGGCCGGAAAACAAAGCGCTGCGGGGGTTCCTCTGCTCGAAAAGGCTGTGGGGGCTTCCAAGGCTCAGACCCTCGGAAACATAATCGTCTCGGTTGTTGGGACCGGCGTCTTGGGACTTCCGTATGCTTTGCGAATCGCTGGTTGGCTTGCTGGCTCGCTCGGCGTTATTGTCATCGGTCTTACAACATATTACTGCATGCTCCTCCTTGTGCAATGTAGGGAGAAATTTGCATCGGAAGAAGATTCAATGGTTAAAAAGTCGTATGGTGATTTGGGGTATGAATGCATGGGAAGAAAAGGTCGTTATCTGACTGAATCCTTGATTTTGATTGCTCAATGCGGGGGCTCTGTGGCGTATCTTGTGTTTGTCGGACAAAATATTTCATCTATGTTCAATGGCCATGGACTCTCGGTTAATTCCTGTATATTTTTGCTAGTGCCAGTGGAAATTGGGTTATCTTGGATAGGTAGTCTATCTGCTTTAGCACCCTTCAGTATCTTTGCTGCCATCTGCAATTTGTTGGCTATGGCAATTGTGGTGAAGCAAGACATACAGCAAGCAGTAGAGGGTGAGTTTTCTTTTAGGGATAGGACGGCGATCACATCAAATATAGGAGGGTTGCCATTTGCAGGAGGCGTGGCAGTGTTTTGTTTTGAGGGGTTCGGGATGACTTTGGCTTTGGAAGCCTCTATGAAAGATAAAACCCAGTTCCCAAGGTTGCTTGCTCAGACTTTCACAGGGATAAGCATTGTGTACGCTTTATTTGGATTCTTTGGATACATGGCTTACGGTGATCAAACGAGAGACATTATCACTCTCAATCTCCCCCGAAATTGGTCATCCATAGCCGTTCAGATTGGCTTGTGCTTGGGTTTAATATTTACATTCCCGATCATGCTCCACCCAATAAACGAGATCATAGAGGGAAACTTAACAAAAGATTCATCTACAAGAATGGGAAAATTGGGAGTATACATGAGTCGAGCAATGGTGGTGATGGTGTTGGCTGTCTTGGCCTCATGTGTGCCAGGATTTGCAGTGTTTGCCTCACTTGTGGGAAGTACAGTATGCGCACTGATATCATTTGTTTTGCCAGCCACATTTCATTTAACGTTATTCGGTTCTTCCCTACAGTTCTGGCAGAAAACCTTGGATTTCTTCATTTTACTGTGTGGATTACTTTTTGCGGTATATGGTACTTTCAATGCCGTAGACGGTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

409

Amino Acids

43.67

Weight (kDa)

7.45

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 21 - 404 2e-93 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47115
fragaria_vesca FvH4_1g26530 FvH4_1g26530
malus_domestica MD09G1288800.v1.1 MD17G1280700.v1.1
prunus_persica Prupe.3G004800_v2.0.a1
pyrus_communis pycom09g19580 pycom17g27850
rosa_chinensis RchiOBHm_Chr3g0486611
rosa_laevigata RLG00000023073
rosa_multiflora Rmu_sc0001508.1_g000003
rosa_roxburghii Rroxscaffold_6G00395770
rosa_rugosa Rorug03G0225600 Rorug03G0225700
rosa_samantha Rh3AG275500 Rh3CG309900 Rh3DG306600
rosa_wichuraiana Rw3G024550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1078
AccI GTMKAC 2 cut(s) 978, 1218
AciI CCGC 3 cut(s) 26, 345, 1193
AclI AACGTT 1 cut(s) 1118
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 1 cut(s) 230
AcuI CTGAAG 1 cut(s) 481
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 3 cut(s) 761, 1069, 1204
AfeI AGCGCT 1 cut(s) 22
AfiI CCNNNNNNNGG 2 cut(s) 635, 840
AgsI TTSAA 3 cut(s) 252, 400, 1210
AjnI CCWGG 1 cut(s) 1033
AjuI GAANNNNNNNTTGG 2 cut(s) 130, 162
Alw26I GTCTC 2 cut(s) 97, 804
Aor51HI AGCGCT 1 cut(s) 22
AoxI GGCC 3 cut(s) 6, 403, 1020
ApeKI GCWGC 2 cut(s) 23, 509
ApoI RAATTY 1 cut(s) 230
ArsI GACNNNNNNTTYG 2 cut(s) 667, 699
AspLEI GCGC 2 cut(s) 23, 1079
AspS9I GGNCC 2 cut(s) 6, 105
AsuHPI GGTGA 5 cut(s) 284, 556, 587, 809, 1012
AvaII GGWCC 1 cut(s) 105
BalI TGGCCA 1 cut(s) 405
BanII GRGCYC 1 cut(s) 353
BbvI GCAGC 2 cut(s) 10, 496
BccI CCATC 2 cut(s) 521, 997
BceAI ACGGC 3 cut(s) 615, 839, 1199
BciT130I CCWGG 1 cut(s) 1035
BciVI GTATCC 1 cut(s) 776
BclI TGATCA 1 cut(s) 799
BcoDI GTCTC 2 cut(s) 97, 804
BfaI CTAG 1 cut(s) 440
BfmI CTRYAG 1 cut(s) 1136
BfoI RGCGCY 1 cut(s) 24
BfuI GTATCC 1 cut(s) 776
BisI GCNGC 2 cut(s) 24, 510
BlsI GCNGC 2 cut(s) 25, 511
Bme1390I CCNGG 1 cut(s) 1035
Bme18I GGWCC 1 cut(s) 105
BmgT120I GGNCC 2 cut(s) 6, 105
BmiI GGNNCC 3 cut(s) 7, 33, 106
BmrFI CCNGG 1 cut(s) 1035
BmrI ACTGGG 1 cut(s) 706
BmsI GCATC 1 cut(s) 245
BmuI ACTGGG 1 cut(s) 706
BoxI GACNNNNGTC 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 5 cut(s) 63, 76, 406, 719, 1155
Bsc4I CCNNNNNNNGG 2 cut(s) 635, 840
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 2 cut(s) 446, 712
Bse3DI GCAATG 2 cut(s) 224, 999
BseBI CCWGG 1 cut(s) 1035
BseDI CCNNGG 5 cut(s) 63, 76, 406, 719, 1155
BseGI GGATG 2 cut(s) 678, 845
BseLI CCNNNNNNNGG 2 cut(s) 635, 840
BseMI GCAATG 2 cut(s) 224, 999
BseMII CTCAG 2 cut(s) 83, 746
BseNI ACTGG 2 cut(s) 446, 712
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 2 cut(s) 10, 496
BshFI GGCC 3 cut(s) 8, 405, 1022
BsiSI CCGG 2 cut(s) 9, 108
BslFI GGGAC 2 cut(s) 118, 132
BslI CCNNNNNNNGG 2 cut(s) 635, 840
BsmAI GTCTC 2 cut(s) 97, 804
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 2 cut(s) 118, 132
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 3 cut(s) 8, 405, 1022
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 4 cut(s) 603, 799, 897, 921
Bsp19I CCATGG 1 cut(s) 406
BspACI CCGC 3 cut(s) 26, 345, 1193
BspANI GGCC 3 cut(s) 8, 405, 1022
BspCNI CTCAG 2 cut(s) 82, 745
BspLI GGNNCC 3 cut(s) 7, 33, 106
BsrDI GCAATG 2 cut(s) 224, 999
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 2 cut(s) 446, 712
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 5 cut(s) 63, 76, 406, 719, 1155
BssMI GATC 4 cut(s) 603, 799, 897, 921
BssNAI GTATAC 1 cut(s) 979
BssNI GRCGYC 1 cut(s) 111
BssT1I CCWWGG 4 cut(s) 63, 406, 719, 1155
Bst1107I GTATAC 1 cut(s) 979
Bst2UI CCWGG 1 cut(s) 1035
Bst4CI ACNGT 5 cut(s) 797, 1072, 1140, 1176, 1223
BstACI GRCGYC 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 1049
BstC8I GCNNGC 7 cut(s) 153, 157, 161, 203, 564, 729, 1101
BstDEI CTNAG 2 cut(s) 69, 732
BstDSI CCRYGG 1 cut(s) 406
BstF5I GGATG 2 cut(s) 678, 845
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 2 cut(s) 23, 1079
BstKTI GATC 4 cut(s) 606, 802, 900, 924
BstMAI GTCTC 2 cut(s) 97, 804
BstMBI GATC 4 cut(s) 603, 799, 897, 921
BstMWI GCNNNNNNNGC 6 cut(s) 56, 65, 165, 689, 1019, 1049
BstNI CCWGG 1 cut(s) 1035
BstNSI RCATGY 1 cut(s) 205
BstPAI GACNNNNGTC 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 1033
BstSFI CTRYAG 1 cut(s) 1136
BstV1I GCAGC 2 cut(s) 10, 496
BstZ17I GTATAC 1 cut(s) 979
BsuI GTATCC 1 cut(s) 776
BsuRI GGCC 3 cut(s) 8, 405, 1022
BtgI CCRYGG 1 cut(s) 406
BtsCI GGATG 2 cut(s) 678, 845
BtsI GCAGTG 2 cut(s) 654, 1050
BtsIMutI CAGTG 4 cut(s) 453, 654, 1050, 1079
Cac8I GCNNGC 7 cut(s) 153, 157, 161, 203, 564, 729, 1101
CfoI GCGC 2 cut(s) 23, 1079
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 2 cut(s) 6, 105
CseI GACGC 1 cut(s) 100
Csp6I GTAC 3 cut(s) 760, 1068, 1203
CviAII CATG 7 cut(s) 202, 292, 407, 787, 901, 982, 1026
CviQI GTAC 3 cut(s) 760, 1068, 1203
DdeI CTNAG 2 cut(s) 69, 732
DpnI GATC 4 cut(s) 605, 801, 899, 923
DpnII GATC 4 cut(s) 603, 799, 897, 921
EaeI YGGCCR 1 cut(s) 403
Eco130I CCWWGG 4 cut(s) 63, 406, 719, 1155
Eco24I GRGCYC 1 cut(s) 353
Eco32I GATATC 1 cut(s) 1086
Eco47I GGWCC 1 cut(s) 105
Eco47III AGCGCT 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 481
EcoRII CCWGG 1 cut(s) 1033
EcoRV GATATC 1 cut(s) 1086
EcoT14I CCWWGG 4 cut(s) 63, 406, 719, 1155
EcoT22I ATGCAT 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 4 cut(s) 63, 406, 719, 1155
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 7 cut(s) 205, 295, 410, 790, 904, 985, 1029
FaqI GGGAC 2 cut(s) 118, 132
FatI CATG 7 cut(s) 201, 291, 406, 786, 900, 981, 1025
FauI CCCGC 2 cut(s) 19, 338
FbaI TGATCA 1 cut(s) 799
FblI GTMKAC 2 cut(s) 978, 1218
Fnu4HI GCNGC 2 cut(s) 24, 510
FokI GGATG 2 cut(s) 685, 832
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 2 cut(s) 24, 510
FspAI RTGCGCAY 1 cut(s) 1078
FspBI CTAG 1 cut(s) 440
FspI TGCGCA 1 cut(s) 1078
GlaI GCGC 2 cut(s) 22, 1078
GluI GCNGC 2 cut(s) 24, 510
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 3 cut(s) 8, 405, 1022
HapII CCGG 2 cut(s) 9, 108
HgaI GACGC 1 cut(s) 100
HhaI GCGC 2 cut(s) 23, 1079
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 7 cut(s) 205, 295, 410, 790, 904, 985, 1029
Hin6I GCGC 2 cut(s) 21, 1077
HinP1I GCGC 2 cut(s) 21, 1077
HinfI GANTC 7 cut(s) 138, 248, 320, 411, 774, 947, 985
HpaII CCGG 2 cut(s) 9, 108
HphI GGTGA 5 cut(s) 284, 556, 587, 809, 1012
Hpy166II GTNNAC 3 cut(s) 760, 979, 1219
Hpy188I TCNGA 7 cut(s) 72, 80, 241, 315, 377, 735, 861
Hpy188III TCNNGA 2 cut(s) 670, 895
Hpy8I GTNNAC 3 cut(s) 760, 979, 1219
HpyAV CCTTC 1 cut(s) 505
HpyCH4III ACNGT 5 cut(s) 797, 1072, 1140, 1176, 1223
HpyCH4IV ACGT 1 cut(s) 1118
HpyCH4V TGCA 7 cut(s) 201, 217, 236, 291, 519, 635, 1043
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 65, 165, 689, 1019, 1049
HpyF3I CTNAG 2 cut(s) 69, 732
HpySE526I ACGT 1 cut(s) 1118
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 7 cut(s) 205, 295, 410, 790, 904, 985, 1029
HspAI GCGC 2 cut(s) 21, 1077
Ksp22I TGATCA 1 cut(s) 799
Kzo9I GATC 4 cut(s) 603, 799, 897, 921
LmnI GCTCC 2 cut(s) 210, 909
Lsp1109I GCAGC 2 cut(s) 10, 496
LweI GCATC 1 cut(s) 245
MaeI CTAG 1 cut(s) 440
MaeII ACGT 1 cut(s) 1118
MalI GATC 4 cut(s) 605, 801, 899, 923
MboI GATC 4 cut(s) 603, 799, 897, 921
MboII GAAGA 5 cut(s) 254, 257, 309, 1122, 1156
MfeI CAATTG 1 cut(s) 537
MhlI GDGCHC 1 cut(s) 353
MlsI TGGCCA 1 cut(s) 405
MluCI AATT 7 cut(s) 230, 421, 453, 520, 537, 838, 968
MluNI TGGCCA 1 cut(s) 405
MlyI GAGTC 2 cut(s) 405, 994
MmeI TCCRAC 1 cut(s) 355
Mox20I TGGCCA 1 cut(s) 405
Mph1103I ATGCAT 1 cut(s) 293
MscI TGGCCA 1 cut(s) 405
MseI TTAA 5 cut(s) 258, 420, 881, 938, 1115
Msp20I TGGCCA 1 cut(s) 405
MspI CCGG 2 cut(s) 9, 108
MspR9I CCNGG 1 cut(s) 1035
MunI CAATTG 1 cut(s) 537
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 1 cut(s) 1035
MwoI GCNNNNNNNGC 6 cut(s) 56, 65, 165, 689, 1019, 1049
NcoI CCATGG 1 cut(s) 406
NdeII GATC 4 cut(s) 603, 799, 897, 921
NlaIII CATG 7 cut(s) 205, 295, 410, 790, 904, 985, 1029
NlaIV GGNNCC 3 cut(s) 7, 33, 106
NmeAIII GCCGAG 1 cut(s) 144
NsbI TGCGCA 1 cut(s) 1078
NsiI ATGCAT 1 cut(s) 293
NspI RCATGY 1 cut(s) 205
PaeI GCATGC 1 cut(s) 205
PctI GAATGC 1 cut(s) 293
PfeI GAWTC 5 cut(s) 138, 248, 320, 774, 947
PkrI GCNGC 2 cut(s) 25, 511
PleI GAGTC 2 cut(s) 405, 993
PpsI GAGTC 2 cut(s) 405, 993
PshAI GACNNNNGTC 1 cut(s) 110
Psp1406I AACGTT 1 cut(s) 1118
Psp6I CCWGG 1 cut(s) 1033
PspGI CCWGG 1 cut(s) 1033
PspN4I GGNNCC 3 cut(s) 7, 33, 106
PspPI GGNCC 2 cut(s) 6, 105
RsaI GTAC 3 cut(s) 761, 1069, 1204
RsaNI GTAC 3 cut(s) 760, 1068, 1203
SaqAI TTAA 5 cut(s) 258, 420, 881, 938, 1115
SatI GCNGC 2 cut(s) 24, 510
Sau3AI GATC 4 cut(s) 603, 799, 897, 921
Sau96I GGNCC 2 cut(s) 6, 105
SchI GAGTC 2 cut(s) 405, 994
ScrFI CCNGG 1 cut(s) 1035
SduI GDGCHC 1 cut(s) 353
SetI ASST 5 cut(s) 307, 475, 725, 1121, 1157
SfaNI GCATC 1 cut(s) 245
SfcI CTRYAG 1 cut(s) 1136
SinI GGWCC 1 cut(s) 105
SphI GCATGC 1 cut(s) 205
Sse9I AATT 7 cut(s) 230, 421, 453, 520, 537, 838, 968
SsiI CCGC 3 cut(s) 26, 345, 1193
SspI AATATT 2 cut(s) 385, 885
SspMI CTAG 1 cut(s) 440
StyD4I CCNGG 1 cut(s) 1033
StyI CCWWGG 4 cut(s) 63, 406, 719, 1155
TaaI ACNGT 5 cut(s) 797, 1072, 1140, 1176, 1223
TaiI ACGT 1 cut(s) 1121
TaqI TCGA 2 cut(s) 42, 988
TaqII GACCGA 1 cut(s) 170
TasI AATT 7 cut(s) 230, 421, 453, 520, 537, 838, 968
TatI WGTACW 1 cut(s) 1067
TfiI GAWTC 5 cut(s) 138, 248, 320, 774, 947
Tru1I TTAA 5 cut(s) 258, 420, 881, 938, 1115
Tru9I TTAA 5 cut(s) 258, 420, 881, 938, 1115
TscAI CASTG 4 cut(s) 453, 654, 1050, 1086
TseI GCWGC 2 cut(s) 23, 509
TspDTI ATGAA 6 cut(s) 300, 378, 713, 939, 1100, 1156
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 4 cut(s) 453, 654, 1050, 1086
VpaK11BI GGWCC 1 cut(s) 105
XapI RAATTY 1 cut(s) 230
XceI RCATGY 1 cut(s) 205
XmiI GTMKAC 2 cut(s) 978, 1218
XspI CTAG 1 cut(s) 440
Zsp2I ATGCAT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.