MD17G1280700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013474
Physical Location & Seq
Forward (+)
34060792 .. 34063901
3110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1280700.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGCGCTGCAAGTTTACTGGTACGACTTCGTCTGCTTCGCCATTGTTGGGGTTTCTTTTGTGGCCGCGTTGTGGGTGATTTGGCGGAAGGAAGGAGCTTCGAAGAGGTTCGAAGACAGCACCATGTACGACAGCCTTCTGGTGACCCGCCCCGAAAGCCATGTGTTTGTCAGCGCCCTGCCGAAGGGCCACGTCAGCACTTCCCAGCTCTGGACTAGCTGCTGGAAAGGGATCCACCCCGGCTGGCTCTTCGCGTCTCGGTTCGTCTCGTTTCTGGCTTTGGCTGGGTTCTTGGCTTGGGATCTTGTCAAATGGAGTCCTTCCATTTTCACTTACTACACCGAGTGGACATTTACACTGGTTATGATCTATTTTGCGCTGGGAACTCTAGTGTCTGGGTACGGTTGTTGGTTATCCTTAAACAAGCCCCCCACTGGAAATGGGACAAGGACGGAAGCTTTGAGAAGGGAAGTGGAAGAGAATAATGGAACTGCAAATACCTATAGGCAAAAGGGAAATAACGGTACCATCAAATTGCAAAGCCAGTATGCTCTGGAGGAAATTCAGAAACGAACAGGATTTTGGGGTTATCTTATGCAAATTGCGTTCCAGACTTGTGCAGGAGCTGTTGTCCTCACAGATATCGTCTTTTGGTGTGTCATCCTTCCATTTTTATCAAATGCTCACCTTGGCCTCAATACGTTGATGTGTTGCATGCATACGCTAAATGCAGCGTTCCTTCTTCTGGATACCATTCTTAACAGCCTTCCATTTCCATGGTTCCGCATTTCATATTTTGTCCTCTGGAGCTGCATCTACATTGCCTTCCAGTGGATCATTCATGCATTAGGTTTCCAATGGTGGCCGTATCCATTCCTTGAGCTCGATACGCCATGGGCTCCACTATGGTATTTTTGTGGGGCCGTGGTTCACATCCCTTGCTATGGGATTTTTGCACTGATTATAAAGGCAAAGTATTCACTTCTGCCCAAATGGTTTCCCCACGCTTTTGTGAGGCCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

39.31

Weight (kDa)

8.61

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47115
fragaria_vesca FvH4_1g26530 FvH4_1g26530
malus_domestica MD09G1288800.v1.1 MD17G1280700.v1.1
prunus_persica Prupe.3G004800_v2.0.a1
pyrus_communis pycom09g19580 pycom17g27850
rosa_chinensis RchiOBHm_Chr3g0486611
rosa_laevigata RLG00000023073
rosa_multiflora Rmu_sc0001508.1_g000003
rosa_roxburghii Rroxscaffold_6G00395770
rosa_rugosa Rorug03G0225600 Rorug03G0225700
rosa_samantha Rh3AG275500 Rh3CG309900 Rh3DG306600
rosa_wichuraiana Rw3G024550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 965
Acc65I GGTACC 1 cut(s) 524
AccB1I GGYRCC 1 cut(s) 524
AccII CGCG 2 cut(s) 68, 254
AciI CCGC 4 cut(s) 66, 85, 148, 784
AclWI GGATC 4 cut(s) 226, 239, 309, 842
AcoI YGGCCR 2 cut(s) 63, 863
AcsI RAATTY 1 cut(s) 561
AdeI CACNNNGTG 1 cut(s) 345
AfaI GTAC 4 cut(s) 23, 128, 401, 526
AfiI CCNNNNNNNGG 8 cut(s) 48, 72, 184, 210, 434, 745, 831, 944
AjiI CACGTC 1 cut(s) 193
AjuI GAANNNNNNNTTGG 2 cut(s) 565, 597
AluBI AGCT 7 cut(s) 98, 208, 219, 458, 626, 810, 883
AluI AGCT 7 cut(s) 98, 208, 219, 458, 626, 810, 883
Alw21I GWGCWC 1 cut(s) 885
Alw26I GTCTC 2 cut(s) 261, 271
AlwI GGATC 4 cut(s) 226, 239, 309, 842
AlwNI CAGNNNCTG 1 cut(s) 626
AoxI GGCC 6 cut(s) 63, 187, 691, 863, 921, 1016
ApeKI GCWGC 4 cut(s) 7, 219, 731, 810
ApoI RAATTY 1 cut(s) 561
ArsI GACNNNNNNTTYG 2 cut(s) 442, 474
Asp700I GAANNNNTTC 1 cut(s) 107
Asp718I GGTACC 1 cut(s) 524
AspLEI GCGC 3 cut(s) 7, 176, 379
AspS9I GGNCC 2 cut(s) 187, 921
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 3 cut(s) 88, 154, 677
AsuII TTCGAA 2 cut(s) 101, 111
BaeI ACNNNNGTAYC 2 cut(s) 391, 424
BamHI GGATCC 1 cut(s) 231
BanI GGYRCC 1 cut(s) 524
BanII GRGCYC 2 cut(s) 885, 901
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 885
BbvI GCAGC 3 cut(s) 206, 743, 797
BccI CCATC 1 cut(s) 536
BceAI ACGGC 2 cut(s) 850, 908
BciVI GTATCC 2 cut(s) 742, 879
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 2 cut(s) 261, 271
BfaI CTAG 3 cut(s) 216, 389, 1024
BfmI CTRYAG 1 cut(s) 502
BfoI RGCGCY 2 cut(s) 8, 177
BfuI GTATCC 2 cut(s) 742, 879
BisI GCNGC 5 cut(s) 8, 66, 220, 732, 811
BlsI GCNGC 5 cut(s) 9, 67, 221, 733, 812
Bme1390I CCNGG 1 cut(s) 240
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 2 cut(s) 187, 921
BmiI GGNNCC 5 cut(s) 233, 526, 782, 900, 922
BmrFI CCNGG 1 cut(s) 240
BmsI GCATC 1 cut(s) 822
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 2 cut(s) 575, 826
Bpu14I TTCGAA 2 cut(s) 101, 111
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 1 cut(s) 240
BsaJI CCNNGG 5 cut(s) 238, 688, 776, 893, 924
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bsc4I CCNNNNNNNGG 8 cut(s) 48, 72, 184, 210, 434, 745, 831, 944
Bse1I ACTGG 5 cut(s) 23, 363, 439, 544, 829
Bse3DI GCAATG 1 cut(s) 819
BseDI CCNNGG 5 cut(s) 238, 688, 776, 893, 924
BseGI GGATG 2 cut(s) 660, 933
BseLI CCNNNNNNNGG 8 cut(s) 48, 72, 184, 210, 434, 745, 831, 944
BseMI GCAATG 1 cut(s) 819
BseNI ACTGG 5 cut(s) 23, 363, 439, 544, 829
BseXI GCAGC 3 cut(s) 206, 743, 797
BseYI CCCAGC 3 cut(s) 204, 284, 379
BsgI GTGCAG 1 cut(s) 639
Bsh1236I CGCG 2 cut(s) 68, 254
BshFI GGCC 6 cut(s) 65, 189, 693, 865, 923, 1018
BshNI GGYRCC 1 cut(s) 524
BsiHKAI GWGCWC 1 cut(s) 885
BsiSI CCGG 1 cut(s) 240
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 8 cut(s) 48, 72, 184, 210, 434, 745, 831, 944
BsmAI GTCTC 2 cut(s) 261, 271
BsmBI CGTCTC 2 cut(s) 261, 271
BsmFI GGGAC 1 cut(s) 457
BsnI GGCC 6 cut(s) 65, 189, 693, 865, 923, 1018
Bsp119I TTCGAA 2 cut(s) 101, 111
Bsp1286I GDGCHC 2 cut(s) 885, 901
Bsp143I GATC 4 cut(s) 231, 301, 366, 834
Bsp19I CCATGG 2 cut(s) 776, 893
BspACI CCGC 4 cut(s) 66, 85, 148, 784
BspANI GGCC 6 cut(s) 65, 189, 693, 865, 923, 1018
BspFNI CGCG 2 cut(s) 68, 254
BspLI GGNNCC 5 cut(s) 233, 526, 782, 900, 922
BspPI GGATC 4 cut(s) 226, 239, 309, 842
BspQI GCTCTTC 1 cut(s) 254
BspT104I TTCGAA 2 cut(s) 101, 111
BspT107I GGYRCC 1 cut(s) 524
BsrDI GCAATG 1 cut(s) 819
BsrI ACTGG 5 cut(s) 23, 363, 439, 544, 829
BssECI CCNNGG 5 cut(s) 238, 688, 776, 893, 924
BssMI GATC 4 cut(s) 231, 301, 366, 834
BssT1I CCWWGG 3 cut(s) 688, 776, 893
Bst4CI ACNGT 2 cut(s) 404, 524
Bst6I CTCTTC 3 cut(s) 98, 254, 471
BstBI TTCGAA 2 cut(s) 101, 111
BstC8I GCNNGC 2 cut(s) 245, 716
BstDSI CCRYGG 3 cut(s) 776, 893, 924
BstEII GGTNACC 1 cut(s) 142
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 2 cut(s) 660, 933
BstFNI CGCG 2 cut(s) 68, 254
BstH2I RGCGCY 2 cut(s) 8, 177
BstHHI GCGC 3 cut(s) 7, 176, 379
BstKTI GATC 4 cut(s) 234, 304, 369, 837
BstMAI GTCTC 2 cut(s) 261, 271
BstMBI GATC 4 cut(s) 231, 301, 366, 834
BstMWI GCNNNNNNNGC 3 cut(s) 156, 195, 889
BstNSI RCATGY 1 cut(s) 718
BstPI GGTNACC 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 238
BstSFI CTRYAG 1 cut(s) 502
BstUI CGCG 2 cut(s) 68, 254
BstV1I GCAGC 3 cut(s) 206, 743, 797
BstV2I GAAGAC 1 cut(s) 120
BstX2I RGATCY 2 cut(s) 231, 301
BstXI CCANNNNNNTGG 1 cut(s) 777
BstYI RGATCY 2 cut(s) 231, 301
BsuI GTATCC 2 cut(s) 742, 879
BsuRI GGCC 6 cut(s) 65, 189, 693, 865, 923, 1018
BtgI CCRYGG 3 cut(s) 776, 893, 924
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 2 cut(s) 660, 933
BtsIMutI CAGTG 4 cut(s) 356, 432, 836, 956
Cac8I GCNNGC 2 cut(s) 245, 716
CaiI CAGNNNCTG 1 cut(s) 626
CfoI GCGC 3 cut(s) 7, 176, 379
Cfr13I GGNCC 2 cut(s) 187, 921
CseI GACGC 1 cut(s) 243
Csp6I GTAC 4 cut(s) 22, 127, 400, 525
CviAII CATG 6 cut(s) 124, 161, 715, 777, 842, 894
CviQI GTAC 4 cut(s) 22, 127, 400, 525
DpnI GATC 4 cut(s) 233, 303, 368, 836
DpnII GATC 4 cut(s) 231, 301, 366, 834
DraIII CACNNNGTG 1 cut(s) 345
EaeI YGGCCR 2 cut(s) 63, 863
Eam1104I CTCTTC 3 cut(s) 98, 254, 471
EarI CTCTTC 3 cut(s) 98, 254, 471
EciI GGCGGA 1 cut(s) 100
Ecl136II GAGCTC 1 cut(s) 883
Eco130I CCWWGG 3 cut(s) 688, 776, 893
Eco147I AGGCCT 1 cut(s) 1018
Eco24I GRGCYC 2 cut(s) 885, 901
Eco32I GATATC 1 cut(s) 643
Eco53kI GAGCTC 1 cut(s) 883
Eco91I GGTNACC 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 883
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 142
EcoRV GATATC 1 cut(s) 643
EcoT14I CCWWGG 3 cut(s) 688, 776, 893
EcoT22I ATGCAT 2 cut(s) 720, 847
EcoT38I GRGCYC 2 cut(s) 885, 901
ErhI CCWWGG 3 cut(s) 688, 776, 893
Esp3I CGTCTC 2 cut(s) 261, 271
FaeI CATG 6 cut(s) 127, 164, 718, 780, 845, 897
FaqI GGGAC 1 cut(s) 457
FatI CATG 6 cut(s) 123, 160, 714, 776, 841, 893
FauI CCCGC 1 cut(s) 155
Fnu4HI GCNGC 5 cut(s) 8, 66, 220, 732, 811
FokI GGATG 2 cut(s) 647, 920
FriOI GRGCYC 2 cut(s) 885, 901
Fsp4HI GCNGC 5 cut(s) 8, 66, 220, 732, 811
FspBI CTAG 3 cut(s) 216, 389, 1024
GlaI GCGC 3 cut(s) 6, 175, 378
GluI GCNGC 5 cut(s) 8, 66, 220, 732, 811
GsaI CCCAGC 3 cut(s) 208, 288, 383
GsuI CTGGAG 2 cut(s) 575, 826
HaeII RGCGCY 2 cut(s) 8, 177
HaeIII GGCC 6 cut(s) 65, 189, 693, 865, 923, 1018
HapII CCGG 1 cut(s) 240
HgaI GACGC 1 cut(s) 243
HhaI GCGC 3 cut(s) 7, 176, 379
Hin1II CATG 6 cut(s) 127, 164, 718, 780, 845, 897
Hin6I GCGC 3 cut(s) 5, 174, 377
HinP1I GCGC 3 cut(s) 5, 174, 377
HindIII AAGCTT 1 cut(s) 456
HinfI GANTC 1 cut(s) 316
HpaII CCGG 1 cut(s) 240
HphI GGTGA 3 cut(s) 88, 154, 677
Hpy166II GTNNAC 3 cut(s) 16, 348, 931
Hpy188I TCNGA 1 cut(s) 567
Hpy188III TCNNGA 5 cut(s) 211, 554, 610, 746, 805
Hpy8I GTNNAC 3 cut(s) 16, 348, 931
HpyCH4III ACNGT 2 cut(s) 404, 524
HpyCH4IV ACGT 2 cut(s) 192, 701
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 195, 889
HpySE526I ACGT 2 cut(s) 192, 701
Hsp92II CATG 6 cut(s) 127, 164, 718, 780, 845, 897
HspAI GCGC 3 cut(s) 5, 174, 377
KpnI GGTACC 1 cut(s) 528
Kzo9I GATC 4 cut(s) 231, 301, 366, 834
LguI GCTCTTC 1 cut(s) 254
LmnI GCTCC 4 cut(s) 95, 623, 807, 904
Lsp1109I GCAGC 3 cut(s) 206, 743, 797
LweI GCATC 1 cut(s) 822
MaeI CTAG 3 cut(s) 216, 389, 1024
MaeII ACGT 2 cut(s) 192, 701
MaeIII GTNAC 1 cut(s) 142
MalI GATC 4 cut(s) 233, 303, 368, 836
MboI GATC 4 cut(s) 231, 301, 366, 834
MboII GAAGA 5 cut(s) 115, 125, 241, 488, 734
MflI RGATCY 2 cut(s) 231, 301
MhlI GDGCHC 2 cut(s) 885, 901
MluCI AATT 3 cut(s) 533, 561, 600
MlyI GAGTC 1 cut(s) 325
MnlI CCTC 6 cut(s) 99, 550, 644, 704, 812, 1008
Mph1103I ATGCAT 2 cut(s) 720, 847
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 2 cut(s) 419, 759
MslI CAYNNNNRTG 1 cut(s) 775
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 1 cut(s) 240
MvnI CGCG 2 cut(s) 68, 254
MwoI GCNNNNNNNGC 3 cut(s) 156, 195, 889
NciI CCSGG 1 cut(s) 240
NcoI CCATGG 2 cut(s) 776, 893
NdeII GATC 4 cut(s) 231, 301, 366, 834
NlaIII CATG 6 cut(s) 127, 164, 718, 780, 845, 897
NlaIV GGNNCC 5 cut(s) 233, 526, 782, 900, 922
NmuCI GTSAC 1 cut(s) 142
NsiI ATGCAT 2 cut(s) 720, 847
NspI RCATGY 1 cut(s) 718
NspV TTCGAA 2 cut(s) 101, 111
PaeI GCATGC 1 cut(s) 718
PceI AGGCCT 1 cut(s) 1018
PciSI GCTCTTC 1 cut(s) 254
PdmI GAANNNNTTC 1 cut(s) 107
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 5 cut(s) 9, 67, 221, 733, 812
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
PsiI TTATAA 1 cut(s) 965
Psp124BI GAGCTC 1 cut(s) 885
PspEI GGTNACC 1 cut(s) 142
PspFI CCCAGC 3 cut(s) 204, 284, 379
PspN4I GGNNCC 5 cut(s) 233, 526, 782, 900, 922
PspPI GGNCC 2 cut(s) 187, 921
PsrI GAACNNNNNNTAC 2 cut(s) 481, 513
PstNI CAGNNNCTG 1 cut(s) 626
PsuI RGATCY 2 cut(s) 231, 301
PsyI GACNNNGTC 1 cut(s) 29
RsaI GTAC 4 cut(s) 23, 128, 401, 526
RsaNI GTAC 4 cut(s) 22, 127, 400, 525
RseI CAYNNNNRTG 1 cut(s) 775
SacI GAGCTC 1 cut(s) 885
SapI GCTCTTC 1 cut(s) 254
SaqAI TTAA 2 cut(s) 419, 759
SatI GCNGC 5 cut(s) 8, 66, 220, 732, 811
Sau3AI GATC 4 cut(s) 231, 301, 366, 834
Sau96I GGNCC 2 cut(s) 187, 921
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 1 cut(s) 240
SduI GDGCHC 2 cut(s) 885, 901
SfaNI GCATC 1 cut(s) 822
SfcI CTRYAG 1 cut(s) 502
SfuI TTCGAA 2 cut(s) 101, 111
SmiMI CAYNNNNRTG 1 cut(s) 775
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
SphI GCATGC 1 cut(s) 718
Sse9I AATT 3 cut(s) 533, 561, 600
SseBI AGGCCT 1 cut(s) 1018
SsiI CCGC 4 cut(s) 66, 85, 148, 784
SspMI CTAG 3 cut(s) 216, 389, 1024
SstI GAGCTC 1 cut(s) 885
StuI AGGCCT 1 cut(s) 1018
StyD4I CCNGG 1 cut(s) 238
StyI CCWWGG 3 cut(s) 688, 776, 893
TaaI ACNGT 2 cut(s) 404, 524
TaiI ACGT 2 cut(s) 195, 704
TaqI TCGA 3 cut(s) 101, 111, 885
TasI AATT 3 cut(s) 533, 561, 600
TauI GCSGC 1 cut(s) 68
Tru1I TTAA 2 cut(s) 419, 759
Tru9I TTAA 2 cut(s) 419, 759
TscAI CASTG 4 cut(s) 363, 439, 836, 963
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 4 cut(s) 7, 219, 731, 810
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 2 cut(s) 780, 830
TspGWI ACGGA 1 cut(s) 467
TspRI CASTG 4 cut(s) 363, 439, 836, 963
Tth111I GACNNNGTC 1 cut(s) 29
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 1 cut(s) 561
XceI RCATGY 1 cut(s) 718
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 3 cut(s) 216, 389, 1024
Zsp2I ATGCAT 2 cut(s) 720, 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.